MD13G1281800.v1.1

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014259
Physical Location & Seq
Forward (+)
40977484 .. 40978760
1277 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1281800.v1.1.491

Sequence Viewer

Length: 462 bp
ATGTCCAAACCCAGAAGCAGAAGAGCCAATCCCACTCTAATTCTCTACCTATCCCTCCTTTTAATCCACATCCACTTCTCAATCTGCAATGGGGTTTCAGTTCTTGACCTGAATCATCCACTCAAAGGAAGCGAAATCGAGGCTGCCATGGGGGGAAGAAGCGTCTGCAGCAAGAAGATTGGGGAGTGCTTGACAGAGCCAGAGATGGAGTCAGAGAGCAGCAGGAGAATTCTGGCAATGCAGAAGAAGTACATAAGCTATGGGACTCTCAACAGGGACTTGGTTCCCTGTGGAAAACCAGGGGCTTCCTACTACAATTGTCACGCTGCGGCGGCGAATCCTTACAACAGAGGGTGTGAGGTCATCACAAGATGTGCAAGAGAAGGATGGCATCAATGGACAAAAGGGAAGGGAAGATCAAATTCTTGTACCATTAGATGGATTTTGTCCACATTGTTGTAG

Protein Analysis

154

Amino Acids

17.05

Weight (kDa)

9.43

Isoelectric Point (pI)

60.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 66 - 127 3.1e-28 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23805 AT4G13950
fragaria_vesca FvH4_4g13250
malus_domestica MD04G1017300.v1.1 MD13G1281800.v1.1
prunus_persica Prupe.1G165400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0413501
rosa_laevigata RLG00000008215
rosa_multiflora Rmu_sc0000883.1_g000006
rosa_roxburghii Rroxscaffold_5G00357400
rosa_rugosa Rorug04G0120700
rosa_samantha Rh4AG180400 Rh4BG179700 Rh4CG191500 Rh4DG175700
rosa_wichuraiana Rw4G015030 Rw4G015080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 438
AciI CCGC 2 cut(s) 329, 332
AcsI RAATTY 2 cut(s) 228, 421
AfaI GTAC 2 cut(s) 251, 430
AfiI CCNNNNNNNGG 2 cut(s) 125, 438
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 1 cut(s) 258
AluI AGCT 1 cut(s) 258
ApeKI GCWGC 4 cut(s) 143, 168, 219, 326
ApoI RAATTY 2 cut(s) 228, 421
BbvI GCAGC 4 cut(s) 130, 180, 231, 313
BccI CCATC 3 cut(s) 199, 381, 432
BciT130I CCWGG 1 cut(s) 300
BfmI CTRYAG 1 cut(s) 166
BisI GCNGC 6 cut(s) 144, 169, 220, 327, 330, 333
BlsI GCNGC 6 cut(s) 145, 170, 221, 328, 331, 334
Bme1390I CCNGG 1 cut(s) 300
BmiI GGNNCC 1 cut(s) 285
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 1 cut(s) 400
BsaJI CCNNGG 2 cut(s) 147, 299
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 438
Bse3DI GCAATG 2 cut(s) 94, 243
BseBI CCWGG 1 cut(s) 300
BseDI CCNNGG 2 cut(s) 147, 299
BseGI GGATG 3 cut(s) 69, 115, 392
BseLI CCNNNNNNNGG 2 cut(s) 125, 438
BseMI GCAATG 2 cut(s) 94, 243
BseXI GCAGC 4 cut(s) 130, 180, 231, 313
BslFI GGGAC 2 cut(s) 277, 290
BslI CCNNNNNNNGG 2 cut(s) 125, 438
BsmFI GGGAC 2 cut(s) 277, 290
Bsp143I GATC 1 cut(s) 416
Bsp19I CCATGG 1 cut(s) 147
BspACI CCGC 2 cut(s) 329, 332
BspLI GGNNCC 1 cut(s) 285
BspMAI CTGCAG 1 cut(s) 170
BspQI GCTCTTC 1 cut(s) 16
BsrDI GCAATG 2 cut(s) 94, 243
BssECI CCNNGG 2 cut(s) 147, 299
BssMI GATC 1 cut(s) 416
BssT1I CCWWGG 1 cut(s) 147
Bst2UI CCWGG 1 cut(s) 300
Bst6I CTCTTC 1 cut(s) 16
BstDSI CCRYGG 1 cut(s) 147
BstF5I GGATG 3 cut(s) 69, 115, 392
BstKTI GATC 1 cut(s) 419
BstMBI GATC 1 cut(s) 416
BstMWI GCNNNNNNNGC 2 cut(s) 168, 332
BstNI CCWGG 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 298
BstSFI CTRYAG 1 cut(s) 166
BstV1I GCAGC 4 cut(s) 130, 180, 231, 313
BtgI CCRYGG 1 cut(s) 147
BtsCI GGATG 3 cut(s) 69, 115, 392
CseI GACGC 1 cut(s) 151
Csp6I GTAC 2 cut(s) 250, 429
CviAII CATG 1 cut(s) 148
CviJI RGCY 5 cut(s) 26, 143, 199, 258, 305
CviKI_1 RGCY 5 cut(s) 26, 143, 199, 258, 305
CviQI GTAC 2 cut(s) 250, 429
DpnI GATC 1 cut(s) 418
DpnII GATC 1 cut(s) 416
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco130I CCWWGG 1 cut(s) 147
EcoRI GAATTC 1 cut(s) 228
EcoRII CCWGG 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 147
FaeI CATG 1 cut(s) 151
FaiI YATR 3 cut(s) 149, 254, 261
FaqI GGGAC 2 cut(s) 277, 290
FatI CATG 1 cut(s) 147
Fnu4HI GCNGC 6 cut(s) 144, 169, 220, 327, 330, 333
FokI GGATG 3 cut(s) 56, 102, 399
Fsp4HI GCNGC 6 cut(s) 144, 169, 220, 327, 330, 333
GluI GCNGC 6 cut(s) 144, 169, 220, 327, 330, 333
HgaI GACGC 1 cut(s) 151
Hin1II CATG 1 cut(s) 151
HinfI GANTC 4 cut(s) 112, 209, 265, 337
Hpy166II GTNNAC 1 cut(s) 450
Hpy188I TCNGA 1 cut(s) 214
Hpy188III TCNNGA 1 cut(s) 104
Hpy8I GTNNAC 1 cut(s) 450
HpyAV CCTTC 2 cut(s) 377, 403
HpyCH4V TGCA 4 cut(s) 87, 168, 241, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 168, 332
Hsp92II CATG 1 cut(s) 151
Kzo9I GATC 1 cut(s) 416
LguI GCTCTTC 1 cut(s) 16
LpnPI CCDG 9 cut(s) 25, 122, 208, 213, 218, 259, 285, 301, 312
Lsp1109I GCAGC 4 cut(s) 130, 180, 231, 313
LweI GCATC 1 cut(s) 400
MaeIII GTNAC 1 cut(s) 320
MalI GATC 1 cut(s) 418
MboI GATC 1 cut(s) 416
MboII GAAGA 5 cut(s) 33, 168, 187, 256, 426
MfeI CAATTG 1 cut(s) 316
MluCI AATT 4 cut(s) 39, 228, 316, 421
MlyI GAGTC 2 cut(s) 218, 259
MnlI CCTC 4 cut(s) 65, 133, 344, 352
MseI TTAA 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 300
MunI CAATTG 1 cut(s) 316
MvaI CCWGG 1 cut(s) 300
MwoI GCNNNNNNNGC 2 cut(s) 168, 332
NcoI CCATGG 1 cut(s) 147
NdeII GATC 1 cut(s) 416
NlaIII CATG 1 cut(s) 151
NlaIV GGNNCC 1 cut(s) 285
NmuCI GTSAC 1 cut(s) 320
PciSI GCTCTTC 1 cut(s) 16
PfeI GAWTC 2 cut(s) 112, 337
PflMI CCANNNNNTGG 1 cut(s) 438
PkrI GCNGC 6 cut(s) 145, 170, 221, 328, 331, 334
PleI GAGTC 2 cut(s) 217, 259
PpsI GAGTC 2 cut(s) 217, 259
Psp6I CCWGG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 1 cut(s) 285
PstI CTGCAG 1 cut(s) 170
RsaI GTAC 2 cut(s) 251, 430
RsaNI GTAC 2 cut(s) 250, 429
SapI GCTCTTC 1 cut(s) 16
SaqAI TTAA 1 cut(s) 62
SatI GCNGC 6 cut(s) 144, 169, 220, 327, 330, 333
Sau3AI GATC 1 cut(s) 416
SchI GAGTC 2 cut(s) 218, 259
ScrFI CCNGG 1 cut(s) 300
SetI ASST 4 cut(s) 51, 111, 260, 363
SfaNI GCATC 1 cut(s) 400
SfcI CTRYAG 1 cut(s) 166
Sse9I AATT 4 cut(s) 39, 228, 316, 421
SsiI CCGC 2 cut(s) 329, 332
StyD4I CCNGG 1 cut(s) 298
StyI CCWWGG 1 cut(s) 147
TaqI TCGA 1 cut(s) 138
TasI AATT 4 cut(s) 39, 228, 316, 421
TatI WGTACW 1 cut(s) 249
TauI GCSGC 2 cut(s) 332, 335
TfiI GAWTC 2 cut(s) 112, 337
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TseFI GTSAC 1 cut(s) 320
TseI GCWGC 4 cut(s) 143, 168, 219, 326
Tsp45I GTSAC 1 cut(s) 320
Van91I CCANNNNNTGG 1 cut(s) 438
XapI RAATTY 2 cut(s) 228, 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.