RLG00000008215

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014259
Physical Location & Seq
Reverse (-)
27245398 .. 27246256
859 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008215

Sequence Viewer

Length: 420 bp
ATGTCCAAACTCAGAGACAATCCCATCTTAATCTTGTATCTTGTCCTCCTCTTAATCCTTACCCTTTTCTCAATCTGCAATGGGGCTTCAGTTCTTGACCTCAGTGCACTAAAAGGAAGTGAAATTGATGGCAAGGTTGGGGACTGCATAACAGAGCCAGAGATGGACTCAGAGATAAGTAGAAGAGTGCTAGCAATGCAGAAGAAGTACATAAGCTATGAGACACTGAGGAGGGACTTGGTTCCTTGTGCAAGGCCAGGGGCTTCATACTACAATTGCCATGCTGTGGCGGCCAACCCTTATAATAGAGGCTGTGAGGTCATCACAGGATGTAGAGATGGGGTTGGATATTCTCTTATGAGGAAGACAGGTAATGTGGGTGGAGGACAGACGGATTGCAGCAATGGACCTAAAAAGTGA

Protein Analysis

140

Amino Acids

15.11

Weight (kDa)

8.47

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 52 - 112 1.2e-27 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23805 AT4G13950
fragaria_vesca FvH4_4g13250
malus_domestica MD04G1017300.v1.1 MD13G1281800.v1.1
prunus_persica Prupe.1G165400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0413501
rosa_laevigata RLG00000008215
rosa_multiflora Rmu_sc0000883.1_g000006
rosa_roxburghii Rroxscaffold_5G00357400
rosa_rugosa Rorug04G0120700
rosa_samantha Rh4AG180400 Rh4BG179700 Rh4CG191500 Rh4DG175700
rosa_wichuraiana Rw4G015030 Rw4G015080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AccB7I CCANNNNNTGG 1 cut(s) 286
AciI CCGC 1 cut(s) 290
AcoI YGGCCR 1 cut(s) 291
AcuI CTGAAG 1 cut(s) 72
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 1 cut(s) 286
AjnI CCWGG 1 cut(s) 256
AluBI AGCT 1 cut(s) 216
AluI AGCT 1 cut(s) 216
Alw21I GWGCWC 1 cut(s) 109
Alw26I GTCTC 2 cut(s) 9, 215
Alw44I GTGCAC 1 cut(s) 105
AoxI GGCC 2 cut(s) 254, 291
ApaLI GTGCAC 1 cut(s) 105
ApeKI GCWGC 1 cut(s) 399
AspS9I GGNCC 1 cut(s) 407
AsuNHI GCTAGC 1 cut(s) 190
AvaII GGWCC 1 cut(s) 407
BaeGI GKGCMC 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 371
Bbv12I GWGCWC 1 cut(s) 109
BbvI GCAGC 1 cut(s) 411
BccI CCATC 4 cut(s) 32, 122, 157, 332
BciT130I CCWGG 1 cut(s) 258
BcoDI GTCTC 2 cut(s) 9, 215
BfaI CTAG 1 cut(s) 191
BisI GCNGC 2 cut(s) 291, 400
BlsI GCNGC 2 cut(s) 292, 401
Bme1390I CCNGG 1 cut(s) 258
Bme18I GGWCC 1 cut(s) 407
BmgT120I GGNCC 1 cut(s) 407
BmiI GGNNCC 1 cut(s) 243
BmrFI CCNGG 1 cut(s) 258
BmtI GCTAGC 1 cut(s) 194
BpiI GAAGAC 1 cut(s) 371
BplI GAGNNNNNCTC 2 cut(s) 152, 184
BsaJI CCNNGG 1 cut(s) 257
Bsc4I CCNNNNNNNGG 1 cut(s) 286
Bse3DI GCAATG 3 cut(s) 85, 201, 409
BseBI CCWGG 1 cut(s) 258
BseDI CCNNGG 1 cut(s) 257
BseGI GGATG 1 cut(s) 335
BseLI CCNNNNNNNGG 1 cut(s) 286
BseMI GCAATG 3 cut(s) 85, 201, 409
BseMII CTCAG 4 cut(s) 25, 115, 183, 218
BseRI GAGGAG 2 cut(s) 38, 244
BseSI GKGCMC 1 cut(s) 109
BseXI GCAGC 1 cut(s) 411
BshFI GGCC 2 cut(s) 256, 293
BsiHKAI GWGCWC 1 cut(s) 109
BslFI GGGAC 2 cut(s) 155, 248
BslI CCNNNNNNNGG 1 cut(s) 286
BsmAI GTCTC 2 cut(s) 9, 215
BsmFI GGGAC 2 cut(s) 155, 248
BsnI GGCC 2 cut(s) 256, 293
Bsp1286I GDGCHC 1 cut(s) 109
BspACI CCGC 1 cut(s) 290
BspANI GGCC 2 cut(s) 256, 293
BspCNI CTCAG 4 cut(s) 24, 114, 182, 219
BspLI GGNNCC 1 cut(s) 243
BspOI GCTAGC 1 cut(s) 194
BsrDI GCAATG 3 cut(s) 85, 201, 409
BssECI CCNNGG 1 cut(s) 257
Bst2UI CCWGG 1 cut(s) 258
Bst6I CTCTTC 1 cut(s) 178
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 4 cut(s) 11, 101, 169, 227
BstF5I GGATG 1 cut(s) 335
BstMAI GTCTC 2 cut(s) 9, 215
BstMWI GCNNNNNNNGC 2 cut(s) 196, 290
BstNI CCWGG 1 cut(s) 258
BstSCI CCNGG 1 cut(s) 256
BstSLI GKGCMC 1 cut(s) 109
BstV1I GCAGC 1 cut(s) 411
BstV2I GAAGAC 1 cut(s) 371
BsuRI GGCC 2 cut(s) 256, 293
BtsCI GGATG 1 cut(s) 335
BtsIMutI CAGTG 2 cut(s) 109, 224
Cac8I GCNNGC 1 cut(s) 192
Cfr13I GGNCC 1 cut(s) 407
Csp6I GTAC 1 cut(s) 208
CviAII CATG 1 cut(s) 281
CviJI RGCY 7 cut(s) 86, 157, 216, 256, 263, 293, 312
CviKI_1 RGCY 7 cut(s) 86, 157, 216, 256, 263, 293, 312
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 4 cut(s) 11, 101, 169, 227
EaeI YGGCCR 1 cut(s) 291
Eam1104I CTCTTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 178
Eco47I GGWCC 1 cut(s) 407
Eco57I CTGAAG 1 cut(s) 72
EcoRII CCWGG 1 cut(s) 256
FaeI CATG 1 cut(s) 284
FaiI YATR 7 cut(s) 149, 212, 219, 268, 282, 303, 359
FaqI GGGAC 2 cut(s) 155, 248
FatI CATG 1 cut(s) 280
Fnu4HI GCNGC 2 cut(s) 291, 400
FokI GGATG 1 cut(s) 342
Fsp4HI GCNGC 2 cut(s) 291, 400
FspBI CTAG 1 cut(s) 191
GluI GCNGC 2 cut(s) 291, 400
HaeIII GGCC 2 cut(s) 256, 293
Hin1II CATG 1 cut(s) 284
HinfI GANTC 1 cut(s) 167
Hpy166II GTNNAC 1 cut(s) 107
Hpy188I TCNGA 2 cut(s) 14, 172
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 1 cut(s) 107
HpyCH4V TGCA 6 cut(s) 78, 107, 147, 199, 251, 399
HpyF10VI GCNNNNNNNGC 2 cut(s) 196, 290
HpyF3I CTNAG 4 cut(s) 11, 101, 169, 227
Hsp92II CATG 1 cut(s) 284
LpnPI CCDG 5 cut(s) 171, 243, 270, 312, 354
Lsp1109I GCAGC 1 cut(s) 411
MaeI CTAG 1 cut(s) 191
MboII GAAGA 3 cut(s) 195, 214, 376
MfeI CAATTG 1 cut(s) 274
MhlI GDGCHC 1 cut(s) 109
MluCI AATT 2 cut(s) 123, 274
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 325
MnlI CCTC 9 cut(s) 56, 59, 110, 222, 225, 302, 310, 354, 377
MseI TTAA 2 cut(s) 29, 53
MspR9I CCNGG 1 cut(s) 258
MunI CAATTG 1 cut(s) 274
MvaI CCWGG 1 cut(s) 258
MwoI GCNNNNNNNGC 2 cut(s) 196, 290
NheI GCTAGC 1 cut(s) 190
NlaIII CATG 1 cut(s) 284
NlaIV GGNNCC 1 cut(s) 243
PflMI CCANNNNNTGG 1 cut(s) 286
PkrI GCNGC 2 cut(s) 292, 401
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
PsiI TTATAA 1 cut(s) 303
Psp6I CCWGG 1 cut(s) 256
PspGI CCWGG 1 cut(s) 256
PspN4I GGNNCC 1 cut(s) 243
PspPI GGNCC 1 cut(s) 407
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
SaqAI TTAA 2 cut(s) 29, 53
SatI GCNGC 2 cut(s) 291, 400
Sau96I GGNCC 1 cut(s) 407
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 1 cut(s) 258
SduI GDGCHC 1 cut(s) 109
SetI ASST 6 cut(s) 102, 138, 218, 321, 373, 412
SinI GGWCC 1 cut(s) 407
Sse9I AATT 2 cut(s) 123, 274
SsiI CCGC 1 cut(s) 290
SspMI CTAG 1 cut(s) 191
StyD4I CCNGG 1 cut(s) 256
TasI AATT 2 cut(s) 123, 274
TatI WGTACW 1 cut(s) 207
TauI GCSGC 1 cut(s) 293
Tru1I TTAA 2 cut(s) 29, 53
Tru9I TTAA 2 cut(s) 29, 53
TscAI CASTG 2 cut(s) 109, 231
TseI GCWGC 1 cut(s) 399
TspDTI ATGAA 1 cut(s) 255
TspGWI ACGGA 1 cut(s) 407
TspRI CASTG 2 cut(s) 109, 231
Van91I CCANNNNNTGG 1 cut(s) 286
VneI GTGCAC 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 407
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.