AT4G16250

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009857
Physical Location & Seq
Reverse (-)
9195247 .. 9199840
4594 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G16250.1

Sequence Viewer

Length: 3495 bp
ATGGTCTCCGGAGGTGGTAGCAAAACCAGCGGTGGAGAGGCAGCTTCCTCAGGCCATCGCCGAAGTCGTCACACCAGCGCTGCAGAACAAGCTCAGTCGTCAGCAAACAAAGCCCTAAGGTCACAGAATCAGCAGCCACAAAACCACGGTGGCGGAACAGAGTCCACAAACAAAGCTATTCAACAGTACACTGTCGACGCGAGACTCCACGCCGTCTTCGAACAATCCGGAGAGTCAGGTAAGTCGTTTGATTACTCACAGTCTCTTAAAACGGCGCCGTACGATTCCTCCGTACCAGAGCAGCAGATCACAGCTTATCTCTCCCGGATCCAACGCGGTGGCTATACCCAGCCTTTTGGCTGCTTGATCGCCGTCGAAGAATCCACTTTCACAATCATCGGTTACAGTGAAAATGCGCGGGAAATGCTAGGGCTCATGTCTCAATCTGTACCAAGCATCGAGGACAAATCAGAGGTTTTAACGATTGGTACGGATTTGCGATCTCTCTTCAAGTCATCGAGCTACCTTCTCCTCGAGCGCGCGTTCGTGGCTCGAGAGATCACGCTTCTGAATCCTATTTGGATTCACTCTAACAACACTGGTAAACCTTTCTACGCGATTCTCCACAGGGTTGATGTTGGAATTTTGATCGATTTAGAGCCGGCTCGAACCGAAGATCCGGCACTTTCAATCGCCGGAGCAGTCCAATCGCAGAAACTTGCGGTACGTGCGATTTCTCATTTACAATCGTTGCCTAGCGGCGACATTAAGCTTCTATGTGACACTGTTGTGGAAAGCGTTAGAGATCTTACTGGCTACGACCGCGTTATGGTGTACAAGTTTCATGAAGATGAACATGGTGAAGTCGTAGCCGAGAGTAAACGGAACGATTTAGAGCCTTACATTGGTCTGCATTATCCCGCTACTGATATTCCTCAGGCATCTCGGTTCTTGTTCAAGCAAAACCGTGTTAGGATGATAGTAGATTGCTATGCGTCACCGGTTCGTGTGGTTCAAGACGATAGGCTCACGCAGTTTATATGCTTGGTGGGTTCGACTTTGCGAGCTCCTCATGGCTGTCATGCTCAATACATGACTAACATGGGCTCTATTGCGTCGTTAGCTATGGCAGTTATAATAAATGGAAACGAAGAAGATGGTAATGGGGTTAATACTGGAGGAAGAAACTCGATGAGGCTTTGGGGTTTAGTTGTTTGCCATCACACATCAGCTCGTTGCATACCTTTTCCTTTGAGGTACGCTTGTGAGTTTCTTATGCAGGCCTTTGGCTTACAGCTAAACATGGAGTTGCAGTTAGCCTTGCAGGTGTCTGAAAAACGCGTTCTGAGAATGCAGACACTATTATGTGATATGCTTCTACGTGACTCACCAGCGGGGATTGTCACGCAGAGGCCTAGTATCATGGATTTAGTAAAATGTAATGGTGCGGCATTTCTTTACCAAGGGAAGTATTATCCGTTGGGTGTGACTCCAACTGATTCTCAGATTAATGACATTGTGGAGTGGTTGGTTGCTAACCATTCTGATTCTACCGGGTTAAGCACAGATAGTTTAGGCGATGCGGGTTATCCTCGGGCAGCTGCTTTGGGAGATGCTGTGTGCGGTATGGCAGTCGCGTGTATCACAAAAAGGGACTTCCTTTTCTGGTTTCGGTCTCATACTGAGAAAGAAATCAAATGGGGAGGGGCTAAGCACCATCCTGAGGACAAAGATGATGGTCAGCGGATGAATCCGCGTTCTTCGTTCCAGACTTTTCTCGAAGTTGTTAAGAGCCGATGTCAGCCATGGGAAACTGCTGAAATGGACGCCATTCACTCGCTCCAGCTTATTCTAAGAGACTCTTTCAAAGAGTCTGAAGCGATGGACTCTAAAGCTGCTGCAGCTGGGGCGGTTCAGCCACATGGAGATGATATGGTACAGCAAGGGATGCAGGAGATAGGTGCAGTTGCAAGAGAGATGGTTAGGCTCATTGAGACTGCGACGGTTCCTATATTTGCTGTGGACATAGACGGTTGCATCAATGGGTGGAACGCCAAGATCGCAGAGCTGACCGGTCTTTCTGTTGAAGACGCTATGGGAAAGTCGCTGGTTCGCGAATTGATATACAAAGAGTACAAAGAAACAGTTGATAGGCTTCTTTCTTGTGCTCTCAAAGGGGATGAAGGCAAGAATGTGGAGGTCAAGCTGAAAACTTTTGGTTCCGAGCTACAAGGAAAAGCAATGTTTGTGGTTGTCAACGCATGTTCAAGCAAGGACTACTTAAACAACATCGTTGGAGTCTGCTTTGTTGGACAAGATGTAACTGGTCATAAAATTGTTATGGACAAGTTCATCAACATACAAGGTGATTACAAGGCCATCATCCATAGCCCGAACCCTCTGATCCCTCCAATCTTTGCAGCGGATGAGAATACGTGCTGCCTTGAGTGGAACACTGCAATGGAAAAGCTCACAGGCTGGCCTCGCAGCGAAGTGATTGGAAAATTACTTGTTAGGGAAGTATTTGGGAGCTATTGCAGACTAAAGGGTCCTGATGCGTTAACTAAGTTCATGATCGTCTTGCATAACGCGATCGGTGGCCAAGATACTGATAAATTCCCATTCCCGTTCTTTGATCGCAAAGGGGAATTCATTCAGGCTCTCCTGACTTTGAACAAACGGGTCAGCATCGATGGCAAAATCATTGGGGCTTTCTGTTTTTTGCAGATACCGAGTCCCGAGCTGCAGCAAGCTCTAGAAGTTCAGAGGAGGCAGGAGAGTGAATATTTCTCAAGGAGGAAAGAGTTGGCTTACATTTTCCAAGTTATAAAGAATCCATTGAGTGGATTGCGTTTCACAAATTCATTGCTGGAAGACATGGATTTAAACGAGGATCAGAAGCAGCTTCTTGAAACGAGTGTTTCATGTGAGAAGCAGATCTCAAAGATTGTAGGAGACATGGACGTCAAAAGCATAGATGACGGTTCATTTCTGCTAGAGAGAACAGAGTTCTTCATTGGCAATGTCACAAATGCAGTGGTAAGCCAAGTCATGTTGGTGGTGAGAGAGAGAAATCTCCAGCTGATCCGTAACATTCCCACGGAGGTCAAATCCATGGCTGTCTACGGTGACCAGATAAGGCTCCAACAGGTTCTCGCAGAATTTCTGCTAAGTATTGTCCGTTATGCACCCATGGAAGGCTCGGTAGAGCTCCATCTATGCCCGACTCTGAACCAAATGGCTGACGGATTCTCCGCTGTACGTTTGGAGTTCAGAATGGCGTGTGCAGGGGAAGGTGTGCCGCCAGAGAAAGTGCAAGACATGTTCCATAGTAGCCGATGGACAAGTCCAGAAGGATTAGGACTAAGCGTTTGCAGAAAGATTTTGAAGCTGATGAACGGAGGGGTTCAGTACATAAGAGAATTCGAACGCTCTTATTTCCTAATCGTTATCGAACTCCCGGTTCCTCTAATGATGATGATGCCCTCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006066 GO:0006082 GO:0006325 GO:0006355 GO:0006464 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006807 GO:0006810 GO:0006833 GO:0006873 GO:0006874 GO:0006875 GO:0006950 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0007602 GO:0007623 GO:0008150 GO:0008152 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009581 GO:0009582 GO:0009583 GO:0009585 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009637 GO:0009638 GO:0009639 GO:0009640 GO:0009642 GO:0009645 GO:0009649 GO:0009687 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009791 GO:0009867 GO:0009881 GO:0009883 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010017 GO:0010018 GO:0010029 GO:0010033 GO:0010114 GO:0010148 GO:0010161 GO:0010202 GO:0010218 GO:0010244 GO:0010374 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010617 GO:0010629 GO:0015979 GO:0016043 GO:0016604 GO:0017006 GO:0017007 GO:0017012 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0019752 GO:0023052 GO:0030003 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031516 GO:0031517 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032787 GO:0032870 GO:0036211 GO:0038023 GO:0042044 GO:0042221 GO:0042592 GO:0042752 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043288 GO:0043412 GO:0043436 GO:0043565 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048511 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048856 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0050896 GO:0051171 GO:0051172 GO:0051179 GO:0051234 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0051480 GO:0051606 GO:0051716 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0060089 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070887 GO:0071214 GO:0071229 GO:0071310 GO:0071395 GO:0071478 GO:0071482 GO:0071489 GO:0071490 GO:0071491 GO:0071495 GO:0071704 GO:0071840 GO:0072503 GO:0072507 GO:0080090 GO:0080134 GO:0090558 GO:0097159 GO:0098771 GO:0104004 GO:1900140 GO:1901363 GO:1901564 GO:1901615 GO:1901700 GO:1901701 GO:1902644 GO:1902679 GO:1903506 GO:1903507 GO:1990841 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
GAF HATPase_c HisKA PAS PAS_2 PHY

Protein Analysis

1164

Amino Acids

129.27

Weight (kDa)

5.81

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 105 - 222 2.8e-36 PAS fold
GAF PF01590 256 - 437 1e-16 GAF domain
PHY PF00360 450 - 625 7.2e-53 Phytochrome region
PAS PF00989 659 - 774 4.7e-24 PAS fold
PAS PF00989 790 - 909 4e-22 PAS fold
HisKA PF00512 932 - 995 3.4e-10 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 1043 - 1154 5e-12 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1136, 2828
AarI CACCTGC 1 cut(s) 1315
AasI GACNNNNNNGTC 2 cut(s) 2547, 2963
AatII GACGTC 1 cut(s) 2967
Acc36I ACCTGC 1 cut(s) 1315
AccB1I GGYRCC 1 cut(s) 274
AccB7I CCANNNNNTGG 1 cut(s) 2843
AccI GTMKAC 2 cut(s) 195, 3123
AccIII TCCGGA 2 cut(s) 8, 227
AclWI GGATC 6 cut(s) 322, 335, 671, 2398, 2901, 3079
AcoI YGGCCR 1 cut(s) 2599
AcsI RAATTY 6 cut(s) 642, 2615, 2648, 2860, 3161, 3422
AcuI CTGAAG 1 cut(s) 1896
AcyI GRCGYC 3 cut(s) 275, 1827, 2964
AfeI AGCGCT 1 cut(s) 79
AfiI CCNNNNNNNGG 5 cut(s) 829, 905, 1723, 2843, 3197
AflIII ACRYGT 2 cut(s) 1339, 3321
AgeI ACCGGT 2 cut(s) 1000, 2072
AleI CACNNNNGTG 1 cut(s) 788
AloI GAACNNNNNNTCC 2 cut(s) 661, 693
Alw21I GWGCWC 3 cut(s) 1069, 2170, 3213
Alw26I GTCTC 8 cut(s) 10, 196, 267, 444, 1680, 1851, 1988, 2949
AlwI GGATC 6 cut(s) 322, 335, 671, 2398, 2901, 3079
Ama87I CYCGRG 4 cut(s) 533, 552, 1593, 2738
Aor13HI TCCGGA 2 cut(s) 8, 227
Aor51HI AGCGCT 1 cut(s) 79
AoxI GGCC 6 cut(s) 52, 1281, 1412, 2376, 2480, 2599
ApoI RAATTY 6 cut(s) 642, 2615, 2648, 2860, 3161, 3422
ArsI GACNNNNNNTTYG 2 cut(s) 3354, 3386
AseI ATTAAT 1 cut(s) 1509
AsiGI ACCGGT 2 cut(s) 1000, 2072
Asp700I GAANNNNTTC 1 cut(s) 2652
AspLEI GCGC 5 cut(s) 80, 277, 418, 540, 542
AspS9I GGNCC 1 cut(s) 2549
AsuC2I CCSGG 3 cut(s) 325, 1555, 3461
AsuHPI GGTGA 6 cut(s) 872, 990, 1380, 2378, 3073, 3140
AsuII TTCGAA 2 cut(s) 219, 3426
AvaI CYCGRG 4 cut(s) 533, 552, 1593, 2738
AvaII GGWCC 1 cut(s) 2549
AxyI CCTNAGG 4 cut(s) 49, 116, 936, 1722
BaeI ACNNNNGTAYC 2 cut(s) 1249, 1282
BalI TGGCCA 1 cut(s) 2601
BamHI GGATCC 1 cut(s) 327
BanI GGYRCC 1 cut(s) 274
BanII GRGCYC 4 cut(s) 435, 1069, 1109, 3213
BbsI GAAGAC 3 cut(s) 208, 2094, 2880
Bbv12I GWGCWC 3 cut(s) 1069, 2170, 3213
BceAI ACGGC 4 cut(s) 197, 262, 288, 356
BcgI CGANNNNNNTGC 2 cut(s) 690, 724
BcnI CCSGG 3 cut(s) 325, 1555, 3461
BcoDI GTCTC 8 cut(s) 10, 196, 267, 444, 1680, 1851, 1988, 2949
BfaI CTAG 5 cut(s) 428, 756, 1416, 2756, 2996
BfmI CTRYAG 3 cut(s) 81, 1899, 2744
BfoI RGCGCY 2 cut(s) 81, 278
BfuAI ACCTGC 1 cut(s) 1315
BglII AGATCT 2 cut(s) 805, 2937
BlpI GCTNAGC 1 cut(s) 1710
Bme1390I CCNGG 3 cut(s) 325, 1555, 3461
Bme18I GGWCC 1 cut(s) 2549
BmeT110I CYCGRG 4 cut(s) 533, 552, 1593, 2738
BmgT120I GGNCC 1 cut(s) 2549
BmiI GGNNCC 7 cut(s) 276, 329, 2007, 2221, 2550, 3143, 3465
BmrFI CCNGG 3 cut(s) 325, 1555, 3461
BmsI GCATC 9 cut(s) 465, 950, 1570, 1603, 1938, 2046, 2545, 2697, 3471
BpiI GAAGAC 3 cut(s) 208, 2094, 2880
BpmI CTGGAG 3 cut(s) 1197, 1826, 3062
Bpu1102I GCTNAGC 1 cut(s) 1710
Bpu14I TTCGAA 2 cut(s) 219, 3426
BpuEI CTTGAG 2 cut(s) 2465, 2776
BpuMI CCSGG 3 cut(s) 325, 1555, 3461
Bsa29I ATCGAT 2 cut(s) 651, 2691
BsaAI YACGTR 3 cut(s) 728, 1382, 2436
BsaHI GRCGYC 3 cut(s) 275, 1827, 2964
BsaI GGTCTC 2 cut(s) 10, 1680
BsaJI CCNNGG 7 cut(s) 145, 1462, 1592, 1805, 3099, 3114, 3192
BsaWI WCCGGW 4 cut(s) 8, 227, 1000, 2072
BsaXI ACNNNNNCTCC 8 cut(s) 272, 302, 1298, 1328, 1602, 1632, 3236, 3266
Bsc4I CCNNNNNNNGG 5 cut(s) 829, 905, 1723, 2843, 3197
Bse118I RCCGGY 3 cut(s) 661, 1000, 2072
Bse1I ACTGG 4 cut(s) 604, 817, 1180, 2329
Bse21I CCTNAGG 4 cut(s) 49, 116, 936, 1722
Bse3DI GCAATG 4 cut(s) 2247, 2466, 2864, 3028
BseAI TCCGGA 2 cut(s) 8, 227
BseCI ATCGAT 2 cut(s) 651, 2691
BseDI CCNNGG 7 cut(s) 145, 1462, 1592, 1805, 3099, 3114, 3192
BseGI GGATG 7 cut(s) 981, 1717, 1752, 1953, 2185, 2382, 2431
BseLI CCNNNNNNNGG 5 cut(s) 829, 905, 1723, 2843, 3197
BseMI GCAATG 4 cut(s) 2247, 2466, 2864, 3028
BseMII CTCAG 7 cut(s) 63, 107, 950, 1337, 1517, 1674, 1713
BseNI ACTGG 4 cut(s) 604, 817, 1180, 2329
BsePI GCGCGC 1 cut(s) 538
BseRI GAGGAG 3 cut(s) 521, 1059, 2782
BseYI CCCAGC 2 cut(s) 348, 1904
BsgI GTGCAG 2 cut(s) 1983, 3306
Bsh1285I CGRYCG 2 cut(s) 823, 2595
BshFI GGCC 6 cut(s) 54, 1283, 1414, 2378, 2482, 2601
BshNI GGYRCC 1 cut(s) 274
BshTI ACCGGT 2 cut(s) 1000, 2072
BshVI ATCGAT 2 cut(s) 651, 2691
BsiEI CGRYCG 2 cut(s) 823, 2595
BsiHKAI GWGCWC 3 cut(s) 1069, 2170, 3213
BsiHKCI CYCGRG 4 cut(s) 533, 552, 1593, 2738
BsiWI CGTACG 1 cut(s) 279
BslFI GGGAC 2 cut(s) 1667, 2721
BslI CCNNNNNNNGG 5 cut(s) 829, 905, 1723, 2843, 3197
BsmAI GTCTC 8 cut(s) 10, 196, 267, 444, 1680, 1851, 1988, 2949
BsmFI GGGAC 2 cut(s) 1667, 2721
BsmI GAATGC 1 cut(s) 1356
BsnI GGCC 6 cut(s) 54, 1283, 1414, 2378, 2482, 2601
Bso31I GGTCTC 2 cut(s) 10, 1680
BsoBI CYCGRG 4 cut(s) 533, 552, 1593, 2738
Bsp119I TTCGAA 2 cut(s) 219, 3426
Bsp1286I GDGCHC 5 cut(s) 435, 1069, 1109, 2170, 3213
Bsp13I TCCGGA 2 cut(s) 8, 227
Bsp1407I TGTACA 1 cut(s) 834
Bsp1720I GCTNAGC 1 cut(s) 1710
Bsp19I CCATGG 3 cut(s) 1805, 3114, 3192
Bsp68I TCGCGA 1 cut(s) 2115
BspANI GGCC 6 cut(s) 54, 1283, 1414, 2378, 2482, 2601
BspCNI CTCAG 7 cut(s) 62, 106, 949, 1338, 1516, 1675, 1714
BspDI ATCGAT 2 cut(s) 651, 2691
BspEI TCCGGA 2 cut(s) 8, 227
BspHI TCATGA 3 cut(s) 844, 2571, 3491
BspLI GGNNCC 7 cut(s) 276, 329, 2007, 2221, 2550, 3143, 3465
BspMAI CTGCAG 3 cut(s) 85, 1903, 2748
BspMI ACCTGC 1 cut(s) 1315
BspPI GGATC 6 cut(s) 322, 335, 671, 2398, 2901, 3079
BspT104I TTCGAA 2 cut(s) 219, 3426
BspT107I GGYRCC 1 cut(s) 274
BspTNI GGTCTC 2 cut(s) 10, 1680
BsrDI GCAATG 4 cut(s) 2247, 2466, 2864, 3028
BsrFI RCCGGY 3 cut(s) 661, 1000, 2072
BsrGI TGTACA 1 cut(s) 834
BsrI ACTGG 4 cut(s) 604, 817, 1180, 2329
BssAI RCCGGY 3 cut(s) 661, 1000, 2072
BssECI CCNNGG 7 cut(s) 145, 1462, 1592, 1805, 3099, 3114, 3192
BssHII GCGCGC 1 cut(s) 538
BssNI GRCGYC 3 cut(s) 275, 1827, 2964
BssT1I CCWWGG 4 cut(s) 1462, 1805, 3114, 3192
Bst6I CTCTTC 1 cut(s) 512
BstACI GRCGYC 3 cut(s) 275, 1827, 2964
BstAPI GCANNNNNTGC 1 cut(s) 1948
BstAUI TGTACA 1 cut(s) 834
BstBAI YACGTR 3 cut(s) 728, 1382, 2436
BstBI TTCGAA 2 cut(s) 219, 3426
BstC8I GCNNGC 6 cut(s) 540, 663, 1065, 1281, 2480, 2751
BstDSI CCRYGG 5 cut(s) 145, 1805, 3099, 3114, 3192
BstEII GGTNACC 1 cut(s) 3128
BstF5I GGATG 7 cut(s) 981, 1717, 1752, 1953, 2185, 2382, 2431
BstH2I RGCGCY 2 cut(s) 81, 278
BstHHI GCGC 5 cut(s) 80, 277, 418, 540, 542
BstMAI GTCTC 8 cut(s) 10, 196, 267, 444, 1680, 1851, 1988, 2949
BstMCI CGRYCG 2 cut(s) 823, 2595
BstNSI RCATGY 2 cut(s) 2265, 3325
BstPI GGTNACC 1 cut(s) 3128
BstSCI CCNGG 3 cut(s) 323, 1553, 3459
BstSFI CTRYAG 3 cut(s) 81, 1899, 2744
BstV2I GAAGAC 3 cut(s) 208, 2094, 2880
BstX2I RGATCY 4 cut(s) 327, 676, 805, 2937
BstXI CCANNNNNNTGG 2 cut(s) 338, 356
BstYI RGATCY 4 cut(s) 327, 676, 805, 2937
Bsu15I ATCGAT 2 cut(s) 651, 2691
Bsu36I CCTNAGG 4 cut(s) 49, 116, 936, 1722
BsuRI GGCC 6 cut(s) 54, 1283, 1414, 2378, 2482, 2601
BsuTUI ATCGAT 2 cut(s) 651, 2691
BtgI CCRYGG 5 cut(s) 145, 1805, 3099, 3114, 3192
BtgZI GCGATG 3 cut(s) 41, 1593, 1895
BtsCI GGATG 7 cut(s) 981, 1717, 1752, 1953, 2185, 2382, 2431
BtsI GCAGTG 2 cut(s) 2454, 3042
BtsIMutI CAGTG 6 cut(s) 189, 412, 597, 783, 2454, 3042
BtuMI TCGCGA 1 cut(s) 2115
BveI ACCTGC 1 cut(s) 1315
Cac8I GCNNGC 6 cut(s) 540, 663, 1065, 1281, 2480, 2751
CciI TCATGA 3 cut(s) 844, 2571, 3491
CfoI GCGC 5 cut(s) 80, 277, 418, 540, 542
Cfr10I RCCGGY 3 cut(s) 661, 1000, 2072
Cfr13I GGNCC 1 cut(s) 2549
ClaI ATCGAT 2 cut(s) 651, 2691
CseI GACGC 5 cut(s) 206, 984, 1104, 1835, 2099
CspAI ACCGGT 2 cut(s) 1000, 2072
CspCI CAANNNNNGTGG 4 cut(s) 2229, 2264, 3018, 3053
DinI GGCGCC 1 cut(s) 276
DraI TTTAAA 1 cut(s) 2886
DrdI GACNNNNNNGTC 2 cut(s) 2547, 2963
DseDI GACNNNNNNGTC 2 cut(s) 2547, 2963
EaeI YGGCCR 1 cut(s) 2599
Eam1104I CTCTTC 1 cut(s) 512
EarI CTCTTC 1 cut(s) 512
EciI GGCGGA 1 cut(s) 168
Ecl136II GAGCTC 2 cut(s) 1067, 3211
Eco130I CCWWGG 4 cut(s) 1462, 1805, 3114, 3192
Eco147I AGGCCT 2 cut(s) 1283, 1414
Eco24I GRGCYC 4 cut(s) 435, 1069, 1109, 3213
Eco31I GGTCTC 2 cut(s) 10, 1680
Eco47I GGWCC 1 cut(s) 2549
Eco47III AGCGCT 1 cut(s) 79
Eco53kI GAGCTC 2 cut(s) 1067, 3211
Eco57I CTGAAG 1 cut(s) 1896
Eco81I CCTNAGG 4 cut(s) 49, 116, 936, 1722
Eco88I CYCGRG 4 cut(s) 533, 552, 1593, 2738
Eco91I GGTNACC 1 cut(s) 3128
EcoICRI GAGCTC 2 cut(s) 1067, 3211
EcoO109I RGGNCCY 1 cut(s) 2549
EcoO65I GGTNACC 1 cut(s) 3128
EcoRI GAATTC 2 cut(s) 2648, 3422
EcoT14I CCWWGG 4 cut(s) 1462, 1805, 3114, 3192
EcoT38I GRGCYC 4 cut(s) 435, 1069, 1109, 3213
EgeI GGCGCC 1 cut(s) 276
EheI GGCGCC 1 cut(s) 276
ErhI CCWWGG 4 cut(s) 1462, 1805, 3114, 3192
FalI AAGNNNNNCTT 4 cut(s) 1846, 1878, 2264, 2296
FaqI GGGAC 2 cut(s) 1667, 2721
FauI CCCGC 4 cut(s) 411, 928, 1387, 1576
FblI GTMKAC 2 cut(s) 195, 3123
FokI GGATG 7 cut(s) 988, 1704, 1759, 1960, 2192, 2369, 2438
FriOI GRGCYC 4 cut(s) 435, 1069, 1109, 3213
FspBI CTAG 5 cut(s) 428, 756, 1416, 2756, 2996
GlaI GCGC 5 cut(s) 79, 276, 417, 539, 541
GsaI CCCAGC 2 cut(s) 352, 1908
GsuI CTGGAG 3 cut(s) 1197, 1826, 3062
HaeII RGCGCY 2 cut(s) 81, 278
HaeIII GGCC 6 cut(s) 54, 1283, 1414, 2378, 2482, 2601
HgaI GACGC 5 cut(s) 206, 984, 1104, 1835, 2099
HhaI GCGC 5 cut(s) 80, 277, 418, 540, 542
Hin1I GRCGYC 3 cut(s) 275, 1827, 2964
Hin6I GCGC 5 cut(s) 78, 275, 416, 538, 540
HinP1I GCGC 5 cut(s) 78, 275, 416, 538, 540
HincII GTYRAC 3 cut(s) 196, 2257, 2562
HindII GTYRAC 3 cut(s) 196, 2257, 2562
HindIII AAGCTT 1 cut(s) 770
HpaI GTTAAC 1 cut(s) 2562
HphI GGTGA 6 cut(s) 872, 990, 1380, 2378, 3073, 3140
Hpy99I CGWCG 4 cut(s) 200, 377, 1120, 2005
HpyAV CCTTC 5 cut(s) 536, 2177, 3191, 3287, 3347
HpyCH4IV ACGT 5 cut(s) 727, 1381, 2435, 2964, 3262
HpySE526I ACGT 5 cut(s) 727, 1381, 2435, 2964, 3262
Hsp92I GRCGYC 3 cut(s) 275, 1827, 2964
HspAI GCGC 5 cut(s) 78, 275, 416, 538, 540
KasI GGCGCC 1 cut(s) 274
Kpn2I TCCGGA 2 cut(s) 8, 227
KroI GCCGGC 1 cut(s) 661
KroNI GCCGGC 1 cut(s) 663
KspAI GTTAAC 1 cut(s) 2562
LmnI GCTCC 6 cut(s) 698, 1072, 1845, 2529, 3147, 3216
LweI GCATC 9 cut(s) 465, 950, 1570, 1603, 1938, 2046, 2545, 2697, 3471
MaeI CTAG 5 cut(s) 428, 756, 1416, 2756, 2996
MaeII ACGT 5 cut(s) 727, 1381, 2435, 2964, 3262
MflI RGATCY 4 cut(s) 327, 676, 805, 2937
MhlI GDGCHC 5 cut(s) 435, 1069, 1109, 2170, 3213
MlsI TGGCCA 1 cut(s) 2601
MluCI AATT 9 cut(s) 642, 2117, 2334, 2504, 2615, 2648, 2860, 3161, 3422
MluI ACGCGT 1 cut(s) 1339
MluNI TGGCCA 1 cut(s) 2601
Mly113I GGCGCC 1 cut(s) 275
MmeI TCCRAC 6 cut(s) 355, 619, 1517, 2275, 2290, 3169
Mox20I TGGCCA 1 cut(s) 2601
MroI TCCGGA 2 cut(s) 8, 227
MroNI GCCGGC 1 cut(s) 661
MroXI GAANNNNTTC 1 cut(s) 2652
MscI TGGCCA 1 cut(s) 2601
MslI CAYNNNNRTG 6 cut(s) 788, 849, 1363, 1926, 2459, 3056
Msp20I TGGCCA 1 cut(s) 2601
MspA1I CMGCKG 8 cut(s) 30, 1394, 1601, 1744, 1904, 2423, 3082, 3257
MspR9I CCNGG 3 cut(s) 325, 1555, 3461
Mva1269I GAATGC 1 cut(s) 1356
NaeI GCCGGC 1 cut(s) 663
NarI GGCGCC 1 cut(s) 275
NciI CCSGG 3 cut(s) 325, 1555, 3461
NcoI CCATGG 3 cut(s) 1805, 3114, 3192
NgoMIV GCCGGC 1 cut(s) 661
NlaIV GGNNCC 7 cut(s) 276, 329, 2007, 2221, 2550, 3143, 3465
NmeAIII GCCGAG 1 cut(s) 898
NmuCI GTSAC 9 cut(s) 68, 120, 779, 996, 1382, 1402, 1486, 3025, 3128
NruI TCGCGA 1 cut(s) 2115
NspI RCATGY 2 cut(s) 2265, 3325
NspV TTCGAA 2 cut(s) 219, 3426
OliI CACNNNNGTG 1 cut(s) 788
PaeR7I CTCGAG 2 cut(s) 533, 552
PagI TCATGA 3 cut(s) 844, 2571, 3491
PaqCI CACCTGC 1 cut(s) 1315
PauI GCGCGC 1 cut(s) 538
PceI AGGCCT 2 cut(s) 1283, 1414
PciI ACATGT 1 cut(s) 3321
PcsI WCGNNNNNNNCGW 3 cut(s) 64, 216, 288
PctI GAATGC 1 cut(s) 1356
PdiI GCCGGC 1 cut(s) 663
PdmI GAANNNNTTC 1 cut(s) 2652
Pfl23II CGTACG 1 cut(s) 279
PflMI CCANNNNNTGG 1 cut(s) 2843
PfoI TCCNGGA 1 cut(s) 323
PinAI ACCGGT 2 cut(s) 1000, 2072
Ple19I CGATCG 1 cut(s) 2595
PluTI GGCGCC 1 cut(s) 278
Ppu21I YACGTR 3 cut(s) 728, 1382, 2436
PpuMI RGGWCCY 1 cut(s) 2549
PscI ACATGT 1 cut(s) 3321
PshBI ATTAAT 1 cut(s) 1509
PsiI TTATAA 2 cut(s) 1136, 2828
Psp124BI GAGCTC 2 cut(s) 1069, 3213
Psp5II RGGWCCY 1 cut(s) 2549
PspEI GGTNACC 1 cut(s) 3128
PspFI CCCAGC 2 cut(s) 348, 1904
PspLI CGTACG 1 cut(s) 279
PspN4I GGNNCC 7 cut(s) 276, 329, 2007, 2221, 2550, 3143, 3465
PspPI GGNCC 1 cut(s) 2549
PspPPI RGGWCCY 1 cut(s) 2549
PspXI VCTCGAGB 1 cut(s) 533
PstI CTGCAG 3 cut(s) 85, 1903, 2748
PsuI RGATCY 4 cut(s) 327, 676, 805, 2937
PteI GCGCGC 1 cut(s) 538
PvuI CGATCG 1 cut(s) 2595
PvuII CAGCTG 3 cut(s) 1601, 1904, 3082
RruI TCGCGA 1 cut(s) 2115
RseI CAYNNNNRTG 6 cut(s) 788, 849, 1363, 1926, 2459, 3056
SacI GAGCTC 2 cut(s) 1069, 3213
SalI GTCGAC 1 cut(s) 194
Sau96I GGNCC 1 cut(s) 2549
ScrFI CCNGG 3 cut(s) 325, 1555, 3461
SduI GDGCHC 5 cut(s) 435, 1069, 1109, 2170, 3213
SfaNI GCATC 9 cut(s) 465, 950, 1570, 1603, 1938, 2046, 2545, 2697, 3471
SfcI CTRYAG 3 cut(s) 81, 1899, 2744
SfoI GGCGCC 1 cut(s) 276
Sfr274I CTCGAG 2 cut(s) 533, 552
SfuI TTCGAA 2 cut(s) 219, 3426
SinI GGWCC 1 cut(s) 2549
SlaI CTCGAG 2 cut(s) 533, 552
SmiMI CAYNNNNRTG 6 cut(s) 788, 849, 1363, 1926, 2459, 3056
SmlI CTYRAG 4 cut(s) 533, 552, 2444, 2791
SmoI CTYRAG 4 cut(s) 533, 552, 2444, 2791
Sse9I AATT 9 cut(s) 642, 2117, 2334, 2504, 2615, 2648, 2860, 3161, 3422
SseBI AGGCCT 2 cut(s) 1283, 1414
SspDI GGCGCC 1 cut(s) 274
SspI AATATT 1 cut(s) 2786
SspMI CTAG 5 cut(s) 428, 756, 1416, 2756, 2996
SstI GAGCTC 2 cut(s) 1069, 3213
StuI AGGCCT 2 cut(s) 1283, 1414
StyD4I CCNGG 3 cut(s) 323, 1553, 3459
StyI CCWWGG 4 cut(s) 1462, 1805, 3114, 3192
TaiI ACGT 5 cut(s) 730, 1384, 2438, 2967, 3265
TaqII GACCGA 1 cut(s) 1662
TasI AATT 9 cut(s) 642, 2117, 2334, 2504, 2615, 2648, 2860, 3161, 3422
TatI WGTACW 4 cut(s) 186, 834, 2133, 3411
TauI GCSGC 3 cut(s) 762, 1451, 3304
TscAI CASTG 6 cut(s) 196, 412, 604, 790, 2461, 3042
TseFI GTSAC 9 cut(s) 68, 120, 779, 996, 1382, 1402, 1486, 3025, 3128
Tsp45I GTSAC 9 cut(s) 68, 120, 779, 996, 1382, 1402, 1486, 3025, 3128
TspGWI ACGGA 9 cut(s) 280, 506, 898, 1467, 3077, 3116, 3170, 3261, 3414
TspRI CASTG 6 cut(s) 196, 412, 604, 790, 2461, 3042
Van91I CCANNNNNTGG 1 cut(s) 2843
VpaK11BI GGWCC 1 cut(s) 2549
VspI ATTAAT 1 cut(s) 1509
XapI RAATTY 6 cut(s) 642, 2615, 2648, 2860, 3161, 3422
XbaI TCTAGA 1 cut(s) 2755
XceI RCATGY 2 cut(s) 2265, 3325
XhoI CTCGAG 2 cut(s) 533, 552
XmiI GTMKAC 2 cut(s) 195, 3123
XmnI GAANNNNTTC 1 cut(s) 2652
XspI CTAG 5 cut(s) 428, 756, 1416, 2756, 2996
ZraI GACGTC 1 cut(s) 2965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.