MD13G1153400.v1.1

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009857
Physical Location & Seq
Reverse (-)
12020456 .. 12026201
5746 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1153400.v1.1.491

Sequence Viewer

Length: 3363 bp
ATGGCGTCCGGCGGTCAGTCGTCGGGCACCAGCAATATCAAAGCTCACCACAACACGGAGTCTATGAGCAAAGCCATTGCTCAGTACACCGTCGATGCTCGGCTGCACACGGTGTTCGAGCAGTCCGGCGAGTCCGGCAAGTCGTTCGACTACTCGCAGAGCATGAAAACCACCAAAGATTCGGTTCCGGAGCAGCAGATTACGGCGTACCTGTCCAGGATTCAGAGGGGCGGCCATGTCCAACCGTTCGGGTGCATGATGGCTGTGGACGAAGCCACGTTTGGAGTCATTGCGTACAGCGAGAACGGCCGTGACATGCTCGACCTAACGCCGCAATCAGTTCCTATCCTTGAGAAGCCGGAGATTCTCACAATTGGGACCGACATCCGTACGCTGTTCACGCCGTCGAGCGCGGTTTTGCTGGAGAAGGCATTTGGCGCTCGGGAGATTACGCTTTTGAACCCGATTTGGATCCACTCCAAGATTTCTGGAAAGCCCTTCTATGCAATTTTGCATAGGATTGATGTTGGGGTCGTGATTGATTTGGAGCCTGCAAGAACAGAGGACCCTGCGCTGTCGATTGCCGGCGCGGTGCAGTCGCAGAAGCTGGCGGTGAGGGCGATCTCGCAGCTGCAGTCACTGCCAGGCGGTGACATTAAGCTTTTGTGTGATACTGTGGTGGAGAGTGTGAGGGAGCTTACTGATTATGATAGAGTTATGGTTTATAAGTTTCATGAGGATGAGCATGGTGAGGTTGTGGCTGAGAGCAAAAGGCCTGACTTGGAGCCGTACATTGGGCTGCACTACCCGGCCACGGATATTCCACAGGCGTCAAGGTTCTTGTTCAGGCAGAACCGGGTTCGAATGATAGTAGATTGTCACGCCAATCCGGTTAATGTGATTCAGGATGAAGGGTTGATGCAGCCTTTGTGCTTGGTCGGATCCACACTAAGAGCCCCTCATGGTTGCCATTCCCAGTACATGGCTAATATGGGGTCCATTGCGTCATTGGCGTTGGCCGTAATCATCAATGGAACTGATGAGGAAGCTCTTGGAGGGAGGAATTCAATGAGATTATGGGGCCTGGTTGTTTGCCATCACACCTCCGCTCGGTATATTCCATTTCCGCTTCGGTATGCTTGTGAGTTTTTAATGCAGGCCTTTGGACTTCAGTTAAATATGGAATTGCAATTGGCTTCACAAATGTCTGAGAAACATGTTTTAAGGACGCAGACTCTGTTGTGTGACATGCTTCTGCGTGACTCCCCGACTGGCATTGTCACTCAAAGTCCTAGTATCATGGACCTTGTTAAGTGTGATGGTGCTGCACTCTACTACCAAGGGAGGTACTACCCGCTTGGTGTGACGCCTACCGAAGCCCAGATAAAGGACATTGTGGAATGGTTGTTGGCTTTCCATGGAAGTTCAACTGGTTTGAGCACAGATAGTTTGGGTGATGCCGGGTACCCTGGAGCTGCCTCTCTTGGTGATGCAGTTTGTGGGATGGCGGCTGCTTATAGTACTAAAAGGGATTTTCTGTTCTGGTTTCGATCCCACACTGGGAAAGAGATCAAATGGGGTGGAGCAAAGCATCATCCAGAGGACAAGGATGATGGGCAGAGGATGCATCCACGCTCTTCATTCAAGGCGTTTCTGGAAGTAGTTAAAAGCCGGAGTTTGCCATGGGAGAATGCAGAAATGGATGCAATACACTCTTTGCAGATTATTTTGCGTGACTCGTTTAAGGACACGGAGACAAACCATTCAAAAGCTGTTATGCAGACCCAGCTTGGTGATCTGGAGTTCCAAGGGATCAATGAGCTCAGCTCTGTAGCAAGAGAAATGGTTAGGTTGATAGAGACTGCAACTGCTCCCATATTTGCTGTCGATATTAATGGCTGTATAAATGGGTGGAATGCAAAGGTTGCAGAGTTGACCAGACTCTCAGTTGAGGAAGCTACCGGGAAGTCTTTGGTTCATGATCTCGTTTACAAAGAATCTGAAGAAATTGTTGACAAACTTCTGTCCCGCGCGTTAAAAGGTGAAGAAGATAAGAATGTGGAAATCAAAATGAGGACATTTGGCCCAGAGCATGATAACAAGCCTGTTTTTGTTGTGGTTAATGCTTGCACTAGCAAGGATTACGCTAATAACATAGTTGGAGTTTGCTTTGTTGGTCAGGATGTTACTGGTCAAAAAGTAGTAATGGACAAATTCATAAAAATACAAGGTGATTACAAGGCCATTGTTCATAGCCCCAATCCTTTGATCCCACCCATATTCGCTTCAGATGATAACACCTGTTGCTCGGAATGGAACACTGCCATGGCAAAGCTCACTGGGTGGAGCCACGGAGAAATCCTTGGAAAAATGTTGGTTGGAGAGGTCTTCGGCAGTTGCTGTCGTATCAAGGGTCCAGATGCTATGACAAAATTCATGATTGTCTTGCACAATGCCATCGAAGGGCTAGACACAGATAAATTCCCCTTTTCATTCTTTGACCGGAATGGGAAATATGTACAAGCTCTCTTGACAGCAAATAAGAGGGTGAATACAGAAGGTCAGGTTATTGGAGCTTTCTGCTTTTTGCAGATTGCTAGTCCAGAACTGCATCAGGCTCTTAATGTTCAGAGGCAACAGGAAAAGGAATGTTTATCTAGGATGAAAGAATTGGCTTACATTTGCCAGGAAGTAAAAAATCCTTTAAGCGGTATACGTTTTACTAACTCACTTTTGGAGGCTACAGACTTAACTGAAGATCAAAGGCAGTTTCTGGAGACTAGTGCTGCTTGTGAGAAGCAAATTTTGAAGATTATAAAAGATGTAGATCTGGATAGTATTGACAATGGATCACTGCAGCTTGAGAAGGCAGAATTCTTACTTGGGGGTGTCATAAATGCTGTTGTTAGCCAAGTAATGTTATTGATGAGAGAAAGAGATCTACAGTTGATTCGGGATATTCCTGAAGAAATCAAAACATTGGCTGTTTATGGTGATCAAGTGAGAATTCAGCAGGTCTTGGCTGATTTCTTATTGAATATGGTACGTTATGCACCCTCTCCTGAAGGCTGGGTGGAGATTCATGTTCTTCCAATCTTGAAGAAAGTACCTGATGGAATCACTCTGGTTCGTACTGAATTCAGGTTGGTATGCCCTGGTGAAGGTCTTCCTCCTCAATTAGTTCAAGACATGTTCCATAGCAGTCAATGGATGACTCAGGAGGGGCTAGGACTGAGCATGTGCAGAAAGATTTTAAAGCTCATGAATGGTGATGTCCAATATATCAGAGAGTCCGAAAGATGTTATTTCTTAATTACTCTCGAGCTTCCTATGCCTCGGAGACCTACAAATAGTAGTGACTAG

Protein Analysis

1121

Amino Acids

124.56

Weight (kDa)

5.6

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 69 - 185 1.4e-36 PAS fold
GAF PF01590 219 - 396 1.4e-15 GAF domain
PHY PF00360 409 - 583 9.4e-55 Phytochrome region
PAS PF00989 614 - 729 1.8e-23 PAS fold
PAS_4 PF08448 622 - 732 7.3e-07 PAS fold
PAS PF00989 745 - 864 2.6e-18 PAS fold
HisKA PF00512 888 - 950 4.4e-13 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 998 - 1109 2e-13 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 726, 2816
Acc36I ACCTGC 1 cut(s) 3004
Acc65I GGTACC 1 cut(s) 1464
AccB1I GGYRCC 2 cut(s) 26, 1464
AccB7I CCANNNNNTGG 1 cut(s) 982
AccBSI CCGCTC 1 cut(s) 1109
AccI GTMKAC 1 cut(s) 2713
AccII CGCG 4 cut(s) 413, 590, 2031, 2033
AccIII TCCGGA 1 cut(s) 187
AclWI GGATC 8 cut(s) 466, 479, 936, 949, 1545, 1820, 2263, 2857
AcoI YGGCCR 4 cut(s) 232, 307, 810, 1017
AcsI RAATTY 8 cut(s) 1063, 2213, 2432, 2480, 2802, 2873, 3006, 3137
AcuI CTGAAG 6 cut(s) 1154, 2022, 2271, 2775, 2985, 3084
AcyI GRCGYC 3 cut(s) 5, 830, 1367
AdeI CACNNNGTG 2 cut(s) 112, 2343
AflIII ACRYGT 2 cut(s) 1216, 3189
AgsI TTSAA 9 cut(s) 460, 1068, 1428, 1645, 1767, 2809, 3037, 3100, 3185
AhlI ACTAGT 1 cut(s) 2780
AjnI CCWGG 6 cut(s) 215, 643, 1083, 1468, 2685, 3154
AjuI GAANNNNNNNTTGG 4 cut(s) 264, 296, 2865, 2897
AloI GAACNNNNNNTCC 2 cut(s) 1523, 1555
Alw21I GWGCWC 2 cut(s) 1442, 1824
Alw26I GTCTC 4 cut(s) 1748, 1853, 2771, 3334
AlwI GGATC 8 cut(s) 466, 479, 936, 949, 1545, 1820, 2263, 2857
AlwNI CAGNNNCTG 5 cut(s) 607, 640, 1237, 2400, 2773
Ama87I CYCGRG 2 cut(s) 441, 3320
Aor13HI TCCGGA 1 cut(s) 187
AoxI GGCC 9 cut(s) 232, 307, 773, 810, 1017, 1081, 1158, 2083, 2241
ApoI RAATTY 8 cut(s) 1063, 2213, 2432, 2480, 2802, 2873, 3006, 3137
AseI ATTAAT 1 cut(s) 1893
Asp700I GAANNNNTTC 1 cut(s) 3165
Asp718I GGTACC 1 cut(s) 1464
AspLEI GCGC 5 cut(s) 413, 440, 574, 590, 2033
AspS9I GGNCC 7 cut(s) 378, 565, 996, 1081, 1303, 2084, 2414
AsuC2I CCSGG 4 cut(s) 809, 857, 1462, 1963
AsuII TTCGAA 1 cut(s) 862
AvaI CYCGRG 2 cut(s) 441, 3320
AvaII GGWCC 5 cut(s) 378, 565, 996, 1303, 2414
BaeGI GKGCMC 1 cut(s) 29
BaeI ACNNNNGTAYC 2 cut(s) 663, 696
BamHI GGATCC 2 cut(s) 471, 941
BanI GGYRCC 2 cut(s) 26, 1464
BanII GRGCYC 2 cut(s) 958, 1824
BbsI GAAGAC 2 cut(s) 2380, 3158
Bbv12I GWGCWC 2 cut(s) 1442, 1824
BccI CCATC 7 cut(s) 253, 1104, 1313, 1498, 1607, 2465, 3107
BceAI ACGGC 6 cut(s) 219, 294, 322, 388, 772, 1004
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 6 cut(s) 217, 645, 1085, 1470, 2687, 3156
BclI TGATCA 1 cut(s) 2995
BcnI CCSGG 4 cut(s) 809, 857, 1462, 1963
BcoDI GTCTC 4 cut(s) 1748, 1853, 2771, 3334
BcuI ACTAGT 1 cut(s) 2780
BfaI CTAG 8 cut(s) 1293, 2133, 2468, 2598, 2658, 2781, 3227, 3361
BfmI CTRYAG 5 cut(s) 632, 1830, 2742, 2855, 2942
BfoI RGCGCY 1 cut(s) 441
BfuAI ACCTGC 1 cut(s) 3004
BglII AGATCT 2 cut(s) 2827, 2938
BlpI GCTNAGC 1 cut(s) 1823
BmcAI AGTACT 1 cut(s) 1522
Bme18I GGWCC 5 cut(s) 378, 565, 996, 1303, 2414
BmeT110I CYCGRG 2 cut(s) 441, 3320
BmgT120I GGNCC 7 cut(s) 378, 565, 996, 1081, 1303, 2084, 2414
BmrI ACTGGG 3 cut(s) 970, 1569, 2349
BmuI ACTGGG 3 cut(s) 970, 1569, 2349
BpiI GAAGAC 2 cut(s) 2380, 3158
BplI GAGNNNNNCTC 2 cut(s) 1811, 1843
BpmI CTGGAG 4 cut(s) 443, 1491, 1820, 2795
Bpu1102I GCTNAGC 1 cut(s) 1823
Bpu14I TTCGAA 1 cut(s) 862
BpuEI CTTGAG 2 cut(s) 371, 2882
BpuMI CCSGG 4 cut(s) 809, 857, 1462, 1963
BsaBI GATNNNNATC 2 cut(s) 470, 2826
BsaHI GRCGYC 3 cut(s) 5, 830, 1367
BsaI GGTCTC 1 cut(s) 3334
BsaWI WCCGGW 3 cut(s) 187, 889, 2502
Bse118I RCCGGY 1 cut(s) 584
Bse1I ACTGG 6 cut(s) 976, 1276, 1435, 1564, 2194, 2344
Bse3DI GCAATG 3 cut(s) 75, 288, 999
Bse8I GATNNNNATC 2 cut(s) 470, 2826
BseAI TCCGGA 1 cut(s) 187
BseBI CCWGG 6 cut(s) 217, 645, 1085, 1470, 2687, 3156
BseJI GATNNNNATC 2 cut(s) 470, 2826
BseMI GCAATG 3 cut(s) 75, 288, 999
BseMII CTCAG 7 cut(s) 95, 753, 1200, 1837, 1959, 3224, 3230
BseNI ACTGG 6 cut(s) 976, 1276, 1435, 1564, 2194, 2344
BseRI GAGGAG 1 cut(s) 3162
BseSI GKGCMC 1 cut(s) 29
BseX3I CGGCCG 1 cut(s) 307
BseYI CCCAGC 2 cut(s) 1785, 3069
BsgI GTGCAG 5 cut(s) 89, 614, 785, 1311, 3262
Bsh1236I CGCG 4 cut(s) 413, 590, 2031, 2033
Bsh1285I CGRYCG 1 cut(s) 310
BshFI GGCC 9 cut(s) 234, 309, 775, 812, 1019, 1083, 1160, 2085, 2243
BshNI GGYRCC 2 cut(s) 26, 1464
BsiEI CGRYCG 1 cut(s) 310
BsiHKAI GWGCWC 2 cut(s) 1442, 1824
BsiHKCI CYCGRG 2 cut(s) 441, 3320
BsiWI CGTACG 1 cut(s) 389
BslFI GGGAC 2 cut(s) 391, 2011
BsmAI GTCTC 4 cut(s) 1748, 1853, 2771, 3334
BsmFI GGGAC 2 cut(s) 391, 2011
BsmI GAATGC 2 cut(s) 1696, 1921
BsnI GGCC 9 cut(s) 234, 309, 775, 812, 1019, 1083, 1160, 2085, 2243
Bso31I GGTCTC 1 cut(s) 3334
BsoBI CYCGRG 2 cut(s) 441, 3320
Bsp119I TTCGAA 1 cut(s) 862
Bsp1286I GDGCHC 4 cut(s) 29, 958, 1442, 1824
Bsp13I TCCGGA 1 cut(s) 187
Bsp1407I TGTACA 1 cut(s) 2518
Bsp1720I GCTNAGC 1 cut(s) 1823
Bsp19I CCATGG 3 cut(s) 1417, 1682, 2325
BspANI GGCC 9 cut(s) 234, 309, 775, 812, 1019, 1083, 1160, 2085, 2243
BspCNI CTCAG 7 cut(s) 94, 754, 1201, 1836, 1958, 3225, 3229
BspEI TCCGGA 1 cut(s) 187
BspFNI CGCG 4 cut(s) 413, 590, 2031, 2033
BspHI TCATGA 4 cut(s) 733, 1978, 2436, 3261
BspMAI CTGCAG 2 cut(s) 636, 2859
BspMI ACCTGC 1 cut(s) 3004
BspPI GGATC 8 cut(s) 466, 479, 936, 949, 1545, 1820, 2263, 2857
BspQI GCTCTTC 1 cut(s) 1642
BspT104I TTCGAA 1 cut(s) 862
BspT107I GGYRCC 2 cut(s) 26, 1464
BspTNI GGTCTC 1 cut(s) 3334
BsrBI CCGCTC 1 cut(s) 1109
BsrDI GCAATG 3 cut(s) 75, 288, 999
BsrFI RCCGGY 1 cut(s) 584
BsrGI TGTACA 1 cut(s) 2518
BsrI ACTGG 6 cut(s) 976, 1276, 1435, 1564, 2194, 2344
BssAI RCCGGY 1 cut(s) 584
BssNAI GTATAC 1 cut(s) 2714
BssNI GRCGYC 3 cut(s) 5, 830, 1367
BssT1I CCWWGG 6 cut(s) 1339, 1417, 1682, 1807, 2325, 2362
Bst1107I GTATAC 1 cut(s) 2714
Bst2UI CCWGG 6 cut(s) 217, 645, 1085, 1470, 2687, 3156
Bst4CI ACNGT 5 cut(s) 91, 112, 246, 676, 2946
Bst6I CTCTTC 1 cut(s) 1642
BstACI GRCGYC 3 cut(s) 5, 830, 1367
BstAPI GCANNNNNTGC 3 cut(s) 640, 1624, 1925
BstAUI TGTACA 1 cut(s) 2518
BstBI TTCGAA 1 cut(s) 862
BstC8I GCNNGC 5 cut(s) 552, 586, 609, 1158, 2128
BstDEI CTNAG 8 cut(s) 81, 762, 950, 1209, 1823, 1945, 3216, 3233
BstDSI CCRYGG 5 cut(s) 813, 1417, 1682, 2325, 2350
BstENI CCTNNNNNAGG 1 cut(s) 3159
BstFNI CGCG 4 cut(s) 413, 590, 2031, 2033
BstH2I RGCGCY 1 cut(s) 441
BstHHI GCGC 5 cut(s) 413, 440, 574, 590, 2033
BstMAI GTCTC 4 cut(s) 1748, 1853, 2771, 3334
BstMCI CGRYCG 1 cut(s) 310
BstNI CCWGG 6 cut(s) 217, 645, 1085, 1470, 2687, 3156
BstNSI RCATGY 5 cut(s) 319, 1220, 1252, 3193, 3241
BstSFI CTRYAG 5 cut(s) 632, 1830, 2742, 2855, 2942
BstSLI GKGCMC 1 cut(s) 29
BstUI CGCG 4 cut(s) 413, 590, 2031, 2033
BstV2I GAAGAC 2 cut(s) 2380, 3158
BstX2I RGATCY 4 cut(s) 471, 941, 2827, 2938
BstYI RGATCY 4 cut(s) 471, 941, 2827, 2938
BstZ17I GTATAC 1 cut(s) 2714
BstZI CGGCCG 1 cut(s) 307
BsuRI GGCC 9 cut(s) 234, 309, 775, 812, 1019, 1083, 1160, 2085, 2243
BtgI CCRYGG 5 cut(s) 813, 1417, 1682, 2325, 2350
BtsI GCAGTG 3 cut(s) 638, 2319, 2852
BtsIMutI CAGTG 5 cut(s) 638, 1557, 2319, 2337, 2852
BveI ACCTGC 1 cut(s) 3004
Cac8I GCNNGC 5 cut(s) 552, 586, 609, 1158, 2128
CaiI CAGNNNCTG 5 cut(s) 607, 640, 1237, 2400, 2773
CciI TCATGA 4 cut(s) 733, 1978, 2436, 3261
CfoI GCGC 5 cut(s) 413, 440, 574, 590, 2033
Cfr10I RCCGGY 1 cut(s) 584
Cfr13I GGNCC 7 cut(s) 378, 565, 996, 1081, 1303, 2084, 2414
CseI GACGC 4 cut(s) 819, 993, 1237, 1375
CspCI CAANNNNNGTGG 2 cut(s) 1561, 1596
DdeI CTNAG 8 cut(s) 81, 762, 950, 1209, 1823, 1945, 3216, 3233
DraI TTTAAA 1 cut(s) 3255
DraIII CACNNNGTG 2 cut(s) 112, 2343
EaeI YGGCCR 4 cut(s) 232, 307, 810, 1017
EagI CGGCCG 1 cut(s) 307
Eam1104I CTCTTC 1 cut(s) 1642
EarI CTCTTC 1 cut(s) 1642
Ecl136II GAGCTC 1 cut(s) 1822
EclXI CGGCCG 1 cut(s) 307
Eco130I CCWWGG 6 cut(s) 1339, 1417, 1682, 1807, 2325, 2362
Eco147I AGGCCT 2 cut(s) 775, 1160
Eco24I GRGCYC 2 cut(s) 958, 1824
Eco31I GGTCTC 1 cut(s) 3334
Eco47I GGWCC 5 cut(s) 378, 565, 996, 1303, 2414
Eco52I CGGCCG 1 cut(s) 307
Eco53kI GAGCTC 1 cut(s) 1822
Eco57I CTGAAG 6 cut(s) 1154, 2022, 2271, 2775, 2985, 3084
Eco88I CYCGRG 2 cut(s) 441, 3320
EcoICRI GAGCTC 1 cut(s) 1822
EcoNI CCTNNNNNAGG 1 cut(s) 3159
EcoO109I RGGNCCY 2 cut(s) 565, 1081
EcoRI GAATTC 4 cut(s) 1063, 2873, 3006, 3137
EcoRII CCWGG 6 cut(s) 215, 643, 1083, 1468, 2685, 3154
EcoT14I CCWWGG 6 cut(s) 1339, 1417, 1682, 1807, 2325, 2362
EcoT22I ATGCAT 1 cut(s) 1629
EcoT38I GRGCYC 2 cut(s) 958, 1824
ErhI CCWWGG 6 cut(s) 1339, 1417, 1682, 1807, 2325, 2362
FaqI GGGAC 2 cut(s) 391, 2011
FauI CCCGC 2 cut(s) 1362, 2036
FbaI TGATCA 1 cut(s) 2995
FblI GTMKAC 1 cut(s) 2713
FriOI GRGCYC 2 cut(s) 958, 1824
FspBI CTAG 8 cut(s) 1293, 2133, 2468, 2598, 2658, 2781, 3227, 3361
GlaI GCGC 5 cut(s) 412, 439, 573, 589, 2032
GsaI CCCAGC 2 cut(s) 1789, 3073
GsuI CTGGAG 4 cut(s) 443, 1491, 1820, 2795
HaeII RGCGCY 1 cut(s) 441
HaeIII GGCC 9 cut(s) 234, 309, 775, 812, 1019, 1083, 1160, 2085, 2243
HgaI GACGC 4 cut(s) 819, 993, 1237, 1375
HhaI GCGC 5 cut(s) 413, 440, 574, 590, 2033
Hin1I GRCGYC 3 cut(s) 5, 830, 1367
Hin6I GCGC 5 cut(s) 411, 438, 572, 588, 2031
HinP1I GCGC 5 cut(s) 411, 438, 572, 588, 2031
HincII GTYRAC 2 cut(s) 1935, 2014
HindII GTYRAC 2 cut(s) 1935, 2014
HindIII AAGCTT 1 cut(s) 659
Hpy166II GTNNAC 7 cut(s) 87, 268, 399, 1935, 1990, 2014, 2714
Hpy8I GTNNAC 7 cut(s) 87, 268, 399, 1935, 1990, 2014, 2714
Hpy99I CGWCG 3 cut(s) 25, 95, 409
HpyAV CCTTC 8 cut(s) 421, 508, 905, 2456, 2552, 2860, 3059, 3155
HpyCH4III ACNGT 5 cut(s) 91, 112, 246, 676, 2946
HpyCH4IV ACGT 3 cut(s) 278, 2716, 3046
HpyF3I CTNAG 8 cut(s) 81, 762, 950, 1209, 1823, 1945, 3216, 3233
HpySE526I ACGT 3 cut(s) 278, 2716, 3046
Hsp92I GRCGYC 3 cut(s) 5, 830, 1367
HspAI GCGC 5 cut(s) 411, 438, 572, 588, 2031
Kpn2I TCCGGA 1 cut(s) 187
KpnI GGTACC 1 cut(s) 1468
KroI GCCGGC 1 cut(s) 584
KroNI GCCGGC 1 cut(s) 586
Ksp22I TGATCA 1 cut(s) 2995
LguI GCTCTTC 1 cut(s) 1642
LmnI GCTCC 9 cut(s) 190, 547, 694, 784, 1472, 1583, 1876, 2346, 2573
MaeI CTAG 8 cut(s) 1293, 2133, 2468, 2598, 2658, 2781, 3227, 3361
MaeII ACGT 3 cut(s) 278, 2716, 3046
MbiI CCGCTC 1 cut(s) 1109
MfeI CAATTG 2 cut(s) 372, 1190
MflI RGATCY 4 cut(s) 471, 941, 2827, 2938
MhlI GDGCHC 4 cut(s) 29, 958, 1442, 1824
MlyI GAGTC 9 cut(s) 68, 140, 294, 1228, 1256, 1730, 1935, 3208, 3299
MmeI TCCRAC 4 cut(s) 265, 919, 2140, 2359
Mph1103I ATGCAT 1 cut(s) 1629
MroI TCCGGA 1 cut(s) 187
MroNI GCCGGC 1 cut(s) 584
MroXI GAANNNNTTC 1 cut(s) 3165
MslI CAYNNNNRTG 2 cut(s) 738, 2324
MspA1I CMGCKG 1 cut(s) 631
MunI CAATTG 2 cut(s) 372, 1190
Mva1269I GAATGC 2 cut(s) 1696, 1921
MvaI CCWGG 6 cut(s) 217, 645, 1085, 1470, 2687, 3156
MvnI CGCG 4 cut(s) 413, 590, 2031, 2033
NaeI GCCGGC 1 cut(s) 586
NciI CCSGG 4 cut(s) 809, 857, 1462, 1963
NcoI CCATGG 3 cut(s) 1417, 1682, 2325
NgoMIV GCCGGC 1 cut(s) 584
NmeAIII GCCGAG 1 cut(s) 79
NsiI ATGCAT 1 cut(s) 1629
NspI RCATGY 5 cut(s) 319, 1220, 1252, 3193, 3241
NspV TTCGAA 1 cut(s) 862
PaeR7I CTCGAG 1 cut(s) 3320
PagI TCATGA 4 cut(s) 733, 1978, 2436, 3261
PceI AGGCCT 2 cut(s) 775, 1160
PciI ACATGT 2 cut(s) 1216, 3189
PciSI GCTCTTC 1 cut(s) 1642
PctI GAATGC 2 cut(s) 1696, 1921
PdiI GCCGGC 1 cut(s) 586
PdmI GAANNNNTTC 1 cut(s) 3165
PfeI GAWTC 8 cut(s) 179, 220, 364, 901, 1997, 2950, 3079, 3117
Pfl23II CGTACG 1 cut(s) 389
PflMI CCANNNNNTGG 1 cut(s) 982
PfoI TCCNGGA 1 cut(s) 215
PleI GAGTC 9 cut(s) 67, 139, 293, 1228, 1256, 1730, 1935, 3208, 3298
PpsI GAGTC 9 cut(s) 67, 139, 293, 1228, 1256, 1730, 1935, 3208, 3298
PpuMI RGGWCCY 1 cut(s) 565
PscI ACATGT 2 cut(s) 1216, 3189
PshBI ATTAAT 1 cut(s) 1893
PsiI TTATAA 2 cut(s) 726, 2816
Psp124BI GAGCTC 1 cut(s) 1824
Psp5II RGGWCCY 1 cut(s) 565
Psp6I CCWGG 6 cut(s) 215, 643, 1083, 1468, 2685, 3154
PspFI CCCAGC 2 cut(s) 1785, 3069
PspGI CCWGG 6 cut(s) 215, 643, 1083, 1468, 2685, 3154
PspLI CGTACG 1 cut(s) 389
PspPI GGNCC 7 cut(s) 378, 565, 996, 1081, 1303, 2084, 2414
PspPPI RGGWCCY 1 cut(s) 565
PstI CTGCAG 2 cut(s) 636, 2859
PstNI CAGNNNCTG 5 cut(s) 607, 640, 1237, 2400, 2773
PsuI RGATCY 4 cut(s) 471, 941, 2827, 2938
PvuII CAGCTG 1 cut(s) 631
RseI CAYNNNNRTG 2 cut(s) 738, 2324
SacI GAGCTC 1 cut(s) 1824
SapI GCTCTTC 1 cut(s) 1642
Sau96I GGNCC 7 cut(s) 378, 565, 996, 1081, 1303, 2084, 2414
ScaI AGTACT 1 cut(s) 1522
SchI GAGTC 9 cut(s) 68, 140, 294, 1228, 1256, 1730, 1935, 3208, 3299
SduI GDGCHC 4 cut(s) 29, 958, 1442, 1824
SfcI CTRYAG 5 cut(s) 632, 1830, 2742, 2855, 2942
Sfr274I CTCGAG 1 cut(s) 3320
SfuI TTCGAA 1 cut(s) 862
SinI GGWCC 5 cut(s) 378, 565, 996, 1303, 2414
SlaI CTCGAG 1 cut(s) 3320
SmiMI CAYNNNNRTG 2 cut(s) 738, 2324
SmlI CTYRAG 3 cut(s) 350, 2861, 3320
SmoI CTYRAG 3 cut(s) 350, 2861, 3320
SpeI ACTAGT 1 cut(s) 2780
SseBI AGGCCT 2 cut(s) 775, 1160
SspMI CTAG 8 cut(s) 1293, 2133, 2468, 2598, 2658, 2781, 3227, 3361
SstI GAGCTC 1 cut(s) 1824
StuI AGGCCT 2 cut(s) 775, 1160
StyI CCWWGG 6 cut(s) 1339, 1417, 1682, 1807, 2325, 2362
TaaI ACNGT 5 cut(s) 91, 112, 246, 676, 2946
TaiI ACGT 3 cut(s) 281, 2719, 3049
TaqII GACCGA 1 cut(s) 395
TatI WGTACW 4 cut(s) 84, 978, 1520, 2518
TauI GCSGC 3 cut(s) 234, 334, 1511
TfiI GAWTC 8 cut(s) 179, 220, 364, 901, 1997, 2950, 3079, 3117
TscAI CASTG 5 cut(s) 645, 1564, 2326, 2344, 2859
TspGWI ACGGA 5 cut(s) 71, 377, 830, 1766, 2367
TspRI CASTG 5 cut(s) 645, 1564, 2326, 2344, 2859
Van91I CCANNNNNTGG 1 cut(s) 982
VpaK11BI GGWCC 5 cut(s) 378, 565, 996, 1303, 2414
VspI ATTAAT 1 cut(s) 1893
XagI CCTNNNNNAGG 1 cut(s) 3159
XapI RAATTY 8 cut(s) 1063, 2213, 2432, 2480, 2802, 2873, 3006, 3137
XceI RCATGY 5 cut(s) 319, 1220, 1252, 3193, 3241
XcmI CCANNNNNNNNNTGG 1 cut(s) 1006
XhoI CTCGAG 1 cut(s) 3320
XmiI GTMKAC 1 cut(s) 2713
XmnI GAANNNNTTC 1 cut(s) 3165
XspI CTAG 8 cut(s) 1293, 2133, 2468, 2598, 2658, 2781, 3227, 3361
ZrmI AGTACT 1 cut(s) 1522
Zsp2I ATGCAT 1 cut(s) 1629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.