Rh4AG259100

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0009857
Physical Location & Seq
Forward (+)
57501404 .. 57507216
5813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG259100.1

Sequence Viewer

Length: 2898 bp
ATGGCTTCAGCAGGAGGAGGAGGCGCCGGCGCCGGCGGCTGGGGGAAGGCTCAGTCGTCTTCGGGCACCAGCAATTTCAAGCAGCACAACAGCAGCACTCCGGCGGAGTCGGTGAGCAAAGCGGTGGCGCAGTACACGGTCGACGCTCGGCTGCACGCCGTGTTCGAGCAGTCCGAGTCCGGTAAGTCTTTCGACTACTCGCACAGCATGAGAAGCACCAAAGAATCGGTGCCGGAGCAGCAAATCACGGCGTACCTGTCCAAAATCCAGAGGGGCGGCCATATCCAGCCGTTCGGGTGCACGATTGCCGTGGACGAGTCCACCTTCGGGGTCATTGCCTACAGCGAGAACGCCCGCGACCTGCTCGACCTAATGCCCCAGTCGGTTCCGGTGCTCGAGAGGCGGGAGATCCTCACAATCGGGACCGACGTCAGGACGCTCTTCACGCCTTCCAGCTCCACTTTGCTGGAGAAGGCGTTTGGCGCTCGGGAGATTACTCTGCTCAACCCAATTTGGATACATTCGAGGGTTTCCGGCAAGCCGTTTTACGCGATTTTGCATAGGATTGATGTAGGGGTTGTCATTGATTTGGAGCCTGCTAGAACAGAGGACCCGGCTCTGTCTATAGCCGGGGCCGTGCAGTCTCAGAAGCTGGCCGTGAGGGCGATTTCGCAGCTGCAGTCACTGCCCGGTGGGGATATTAAGCTTTTGTGCGACACGGTGGTGGAGAGTGTGAGGGAGCTTACTGGGTATGATAGAGTTATGGTGTACAAGTTTCATGAGGATGAGCACGGGGAGGTTTTGGCGGAGAGTAAGAGGCCGGATTTGGAGCCTTATCTTGGGCTGCATTATCCGGCCACGGACATACCTCAGGCGTCGCGGTTTTTGTTCAAGCAAAATAGGGTGAGAATGATTGTGGATTGTCATGCCACGCCGGTTCATGTGATCCAGGATGAAGGGCTTATGCAGCCTCTGTGCTTGGTTGGATCAACGCTGCGCGCGCCGCATGGTTGTCATTCCCAGTATATGGCTAATATGGGCTCCATTGCGTCCTTGGCTTTGGCGGTTATCATTAATGGAAATGATGATGAGGCTATTGGTGGACGGAGCTCGATGAGGCTGTGGGGCTTGGTTGTTTGCCATCACACTTCTGCGAGGTGCATCCCGTTTCCTCTTCGGTATGCTTGTGAGTTTCTAATGCAGGCCTTTGGACTACAGTTGAATATGGAATTGCAGTTGGCTTCACAGATGTCGGAGAAACATGTTTTGAGGACTCAGACTCTGTTGTGTGATATGCTTCTTCGTGACACCCCGACTGGGATTATTACTCAAAGTCCTAGTATCATGAATCTTGTCAAGTGTGATGGGGCAGCGCTTTACTACCAAGACAAGTACTACCCAATTGGTGTCACCCCCACTGAAGCTCAGATCAGGGACATTGTTGAGTGGTTGTTGGCTTCACATGGAACTTCGACAGGTTTAAGCACTGACAGTTTGGCTGATGCTGGGTACCCTGGAGCTGCCTCACTTGGTGATGCTGTCTGTGGGATGGCTGCTGCTTATATTACTAAAAGGGACTTTCTCTTCTGGTTCCGATCCCACACTGCAAAAGAGGTGAAATGGGGTGGAGCAAAGCATCATCCTGAGGACAAGGATGATGGGCAGAGGATGCATCCACGTTCTTCCTTTAAGGCGTTTTTGGAAGTGGTTAAAAGCCGTAGTTTGCCATGGGACAATGCAGAAATGGATGCCATACATTCCTTGCAAATTATTTTGCGAGACTCATTTAAGGATGCTGAGACAAACAACTTAAAGGCTGTTACACAGGCCCAGCGTGGGGATCTGGAATATCAAGGGATTGACGAGCTCAGCTCAGTGGCAAGAGAAATGGTTAGATTGATAGAGACTGCAACTGCTCCCATATTTGCTGTAGATGTTGATGGGTGTATAAATGGGTGGAATGCAAAGGTTGCAGAGCTGACAGGGCTCTCAGTTGAGGAGGCTACAGGGAAATCCTTGGTACATGATCTGGTTTACAAAGAATCTGAAGAAACTGTTGAGAAACTTCTCCTCCACGCTCTGAGAGGAGAAGAAGATAAAAATGTAGAGATAAAACTGAGGACATTTGACCCAGAGCATGAAAACAAGCCTGTTTTTGTGGTGGTTAATGCTTGCTCTAGCAAGGATTACACTAATAATATCGTGGGAGTTTGCTTTGTTGGTCAGGATGTTACTGGTCAAAAAGTGGTAATGGACAAATTCATAAACATACAAGGTGATTACAAGGCCATTGTTCATAGTCCCAATGCTTTGATCCCGCCCATATTTGCGTCAGATGATAACACATGTTGCTCAGAGTGGAACACTGCCATGGAAAAACTTACTGGGTGGAGCCGTGGAGATATCATTGGAAAAATGTTGGTTGGGGAGGTCTTTGGCAGTTGTTGTCGACTCAAGGGTCCAGATGCTATAACAAAATTCATGATTGTCTTGCACAACGCCATTGGAGGCTTAGACACGGACAAGTTTCCTTTTTCATTCTTTGACCAGAATGGGAAATATGTACAAGCTCTCTTGACAGCAAATAAGAGGGTGAATATAGACGGCCAGGTTATTGGAGCTTTCTGCTTTCTGCAGATTCCTAGTCCAGAACTGCAGCAAGCTCTTAGAGTTCAGAGACAACAGGAGAAGCAATGTTTCACTAGGATGAAAGAATTCGCTTACATATGTCAGGAAATAAAAAATCCTTTAAGTGGTATACGCTTTACTAACTCACTTTTGGAGGCTACAGAGTTGACTGAAGATCAAAAGCAGTTCTTGGAGACTAGTGGTGCTTGTGAGAAGCAAATTTTGAAGATTATAAAGGACGTTGATCTGGCTAGCATTGAAGATGGGTAA

Protein Analysis

965

Amino Acids

106.29

Weight (kDa)

5.59

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 84 - 200 2.2e-37 PAS fold
GAF PF01590 234 - 411 3.2e-16 GAF domain
PHY PF00360 424 - 598 9e-58 Phytochrome region
PAS PF00989 629 - 744 4e-24 PAS fold
PAS_4 PF08448 637 - 747 1.3e-06 PAS fold
PAS PF00989 760 - 879 3.7e-20 PAS fold
HisKA PF00512 904 - 965 1.6e-13 His Kinase A (phospho-acceptor) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2861
AasI GACNNNNNNGTC 1 cut(s) 2457
AatII GACGTC 1 cut(s) 432
Acc36I ACCTGC 1 cut(s) 369
Acc65I GGTACC 1 cut(s) 1509
AccB1I GGYRCC 5 cut(s) 23, 29, 65, 229, 1509
AccB7I CCANNNNNTGG 1 cut(s) 1027
AccI GTMKAC 3 cut(s) 141, 2449, 2758
AccII CGCG 5 cut(s) 357, 551, 880, 999, 1001
AclWI GGATC 6 cut(s) 403, 940, 994, 1590, 1848, 2308
AcoI YGGCCR 4 cut(s) 277, 654, 855, 2605
AcsI RAATTY 4 cut(s) 2258, 2477, 2714, 2847
AcuI CTGAAG 3 cut(s) 1440, 2067, 2820
AcyI GRCGYC 4 cut(s) 24, 30, 429, 875
AdeI CACNNNGTG 2 cut(s) 160, 1532
AfaI GTAC 7 cut(s) 134, 254, 770, 1394, 1511, 2022, 2565
AfeI AGCGCT 1 cut(s) 1374
AfiI CCNNNNNNNGG 9 cut(s) 39, 327, 432, 839, 1027, 1317, 1836, 1837, 2753
AflIII ACRYGT 2 cut(s) 1261, 2345
AgsI TTSAA 5 cut(s) 79, 892, 1222, 2854, 2888
AhlI ACTAGT 1 cut(s) 2825
AjnI CCWGG 3 cut(s) 948, 1513, 2607
AleI CACNNNNGTG 1 cut(s) 722
Alw21I GWGCWC 5 cut(s) 302, 396, 792, 1112, 1869
Alw26I GTCTC 6 cut(s) 648, 1773, 1793, 1898, 2671, 2816
Alw44I GTGCAC 1 cut(s) 298
AlwI GGATC 6 cut(s) 403, 940, 994, 1590, 1848, 2308
AlwNI CAGNNNCTG 4 cut(s) 652, 685, 973, 1282
Ama87I CYCGRG 2 cut(s) 395, 486
Aor51HI AGCGCT 1 cut(s) 1374
AoxI GGCC 9 cut(s) 277, 633, 654, 818, 855, 1203, 1827, 2286, 2605
ApaLI GTGCAC 1 cut(s) 298
ApoI RAATTY 4 cut(s) 2258, 2477, 2714, 2847
AseI ATTAAT 1 cut(s) 1074
Asp700I GAANNNNTTC 1 cut(s) 2714
Asp718I GGTACC 1 cut(s) 1509
AspLEI GCGC 8 cut(s) 26, 32, 130, 485, 999, 1001, 1003, 1375
AspS9I GGNCC 5 cut(s) 423, 610, 633, 1828, 2459
AsuC2I CCSGG 3 cut(s) 614, 631, 690
AsuHPI GGTGA 7 cut(s) 124, 916, 1402, 1544, 1627, 2288, 2605
AsuNHI GCTAGC 1 cut(s) 2879
AvaI CYCGRG 2 cut(s) 395, 486
AvaII GGWCC 3 cut(s) 423, 610, 2459
AxyI CCTNAGG 2 cut(s) 870, 1644
BaeGI GKGCMC 2 cut(s) 68, 302
BanI GGYRCC 5 cut(s) 23, 29, 65, 229, 1509
BanII GRGCYC 4 cut(s) 1043, 1112, 1869, 1989
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 5 cut(s) 302, 396, 792, 1112, 1869
BccI CCATC 6 cut(s) 1149, 1358, 1543, 1652, 1934, 2885
BciT130I CCWGG 3 cut(s) 950, 1515, 2609
BciVI GTATCC 1 cut(s) 510
BcnI CCSGG 3 cut(s) 614, 631, 690
BcoDI GTCTC 6 cut(s) 648, 1773, 1793, 1898, 2671, 2816
BcuI ACTAGT 1 cut(s) 2825
BfaI CTAG 7 cut(s) 600, 1338, 2178, 2643, 2703, 2826, 2880
BfmI CTRYAG 9 cut(s) 340, 624, 677, 1214, 1929, 2004, 2633, 2654, 2787
BfoI RGCGCY 4 cut(s) 27, 33, 486, 1376
BfuAI ACCTGC 1 cut(s) 369
BfuI GTATCC 1 cut(s) 510
BglI GCCNNNNNGGC 1 cut(s) 662
BlpI GCTNAGC 1 cut(s) 1868
BmcAI AGTACT 1 cut(s) 1394
Bme1390I CCNGG 6 cut(s) 614, 631, 690, 950, 1515, 2609
Bme18I GGWCC 3 cut(s) 423, 610, 2459
BmeT110I CYCGRG 2 cut(s) 395, 486
BmgT120I GGNCC 5 cut(s) 423, 610, 633, 1828, 2459
BmrFI CCNGG 6 cut(s) 614, 631, 690, 950, 1515, 2609
BmrI ACTGGG 5 cut(s) 373, 756, 1015, 1326, 2394
BmsI GCATC 9 cut(s) 1170, 1492, 1525, 1645, 1659, 1681, 1738, 1783, 2455
BmtI GCTAGC 1 cut(s) 2883
BmuI ACTGGG 5 cut(s) 373, 756, 1015, 1326, 2394
BoxI GACNNNNGTC 1 cut(s) 428
BpiI GAAGAC 1 cut(s) 51
BplI GAGNNNNNCTC 2 cut(s) 1856, 1888
BpmI CTGGAG 2 cut(s) 488, 1536
Bpu1102I GCTNAGC 1 cut(s) 1868
BpuEI CTTGAG 1 cut(s) 2438
BpuMI CCSGG 3 cut(s) 614, 631, 690
BsaHI GRCGYC 4 cut(s) 24, 30, 429, 875
BsaJI CCNNGG 9 cut(s) 309, 630, 858, 1053, 1513, 1727, 2016, 2370, 2395
BsaWI WCCGGW 2 cut(s) 179, 388
BsaXI ACNNNNNCTCC 4 cut(s) 461, 491, 2055, 2085
Bsc4I CCNNNNNNNGG 9 cut(s) 39, 327, 432, 839, 1027, 1317, 1836, 1837, 2753
Bse118I RCCGGY 3 cut(s) 26, 32, 934
Bse1I ACTGG 6 cut(s) 379, 751, 1021, 1321, 2239, 2389
Bse21I CCTNAGG 2 cut(s) 870, 1644
Bse3DI GCAATG 3 cut(s) 333, 1044, 2699
BseBI CCWGG 3 cut(s) 950, 1515, 2609
BseDI CCNNGG 9 cut(s) 309, 630, 858, 1053, 1513, 1727, 2016, 2370, 2395
BseLI CCNNNNNNNGG 9 cut(s) 39, 327, 432, 839, 1027, 1317, 1836, 1837, 2753
BseMI GCAATG 3 cut(s) 333, 1044, 2699
BseNI ACTGG 6 cut(s) 379, 751, 1021, 1321, 2239, 2389
BsePI GCGCGC 2 cut(s) 997, 999
BseRI GAGGAG 5 cut(s) 30, 33, 2012, 2060, 2100
BseSI GKGCMC 2 cut(s) 68, 302
BseYI CCCAGC 3 cut(s) 39, 1505, 1830
BsgI GTGCAG 2 cut(s) 137, 659
Bsh1236I CGCG 5 cut(s) 357, 551, 880, 999, 1001
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 9 cut(s) 279, 635, 656, 820, 857, 1205, 1829, 2288, 2607
BshNI GGYRCC 5 cut(s) 23, 29, 65, 229, 1509
BsiEI CGRYCG 1 cut(s) 141
BsiHKAI GWGCWC 5 cut(s) 302, 396, 792, 1112, 1869
BsiHKCI CYCGRG 2 cut(s) 395, 486
BslFI GGGAC 5 cut(s) 436, 1448, 1589, 1745, 2286
BslI CCNNNNNNNGG 9 cut(s) 39, 327, 432, 839, 1027, 1317, 1836, 1837, 2753
BsmAI GTCTC 6 cut(s) 648, 1773, 1793, 1898, 2671, 2816
BsmFI GGGAC 5 cut(s) 436, 1448, 1589, 1745, 2286
BsmI GAATGC 1 cut(s) 1966
BsnI GGCC 9 cut(s) 279, 635, 656, 820, 857, 1205, 1829, 2288, 2607
BsoBI CYCGRG 2 cut(s) 395, 486
Bsp1286I GDGCHC 8 cut(s) 68, 302, 396, 792, 1043, 1112, 1869, 1989
Bsp1407I TGTACA 2 cut(s) 768, 2563
Bsp1720I GCTNAGC 1 cut(s) 1868
Bsp19I CCATGG 2 cut(s) 1727, 2370
BspANI GGCC 9 cut(s) 279, 635, 656, 820, 857, 1205, 1829, 2288, 2607
BspFNI CGCG 5 cut(s) 357, 551, 880, 999, 1001
BspHI TCATGA 3 cut(s) 778, 1344, 2481
BspMAI CTGCAG 3 cut(s) 681, 2637, 2658
BspMI ACCTGC 1 cut(s) 369
BspOI GCTAGC 1 cut(s) 2883
BspPI GGATC 6 cut(s) 403, 940, 994, 1590, 1848, 2308
BspQI GCTCTTC 1 cut(s) 446
BspT107I GGYRCC 5 cut(s) 23, 29, 65, 229, 1509
BsrDI GCAATG 3 cut(s) 333, 1044, 2699
BsrFI RCCGGY 3 cut(s) 26, 32, 934
BsrGI TGTACA 2 cut(s) 768, 2563
BsrI ACTGG 6 cut(s) 379, 751, 1021, 1321, 2239, 2389
BssAI RCCGGY 3 cut(s) 26, 32, 934
BssECI CCNNGG 9 cut(s) 309, 630, 858, 1053, 1513, 1727, 2016, 2370, 2395
BssHII GCGCGC 2 cut(s) 997, 999
BssNAI GTATAC 1 cut(s) 2759
BssNI GRCGYC 4 cut(s) 24, 30, 429, 875
BssT1I CCWWGG 4 cut(s) 1053, 1727, 2016, 2370
Bst1107I GTATAC 1 cut(s) 2759
Bst2UI CCWGG 3 cut(s) 950, 1515, 2609
Bst4CI ACNGT 5 cut(s) 139, 721, 1218, 1493, 2056
Bst6I CTCTTC 3 cut(s) 446, 1179, 1589
BstACI GRCGYC 4 cut(s) 24, 30, 429, 875
BstAPI GCANNNNNTGC 3 cut(s) 685, 1669, 1970
BstAUI TGTACA 2 cut(s) 768, 2563
BstDSI CCRYGG 5 cut(s) 309, 858, 1727, 2370, 2395
BstFNI CGCG 5 cut(s) 357, 551, 880, 999, 1001
BstH2I RGCGCY 4 cut(s) 27, 33, 486, 1376
BstHHI GCGC 8 cut(s) 26, 32, 130, 485, 999, 1001, 1003, 1375
BstMAI GTCTC 6 cut(s) 648, 1773, 1793, 1898, 2671, 2816
BstMCI CGRYCG 1 cut(s) 141
BstNI CCWGG 3 cut(s) 950, 1515, 2609
BstNSI RCATGY 2 cut(s) 1265, 2349
BstPAI GACNNNNGTC 1 cut(s) 428
BstSCI CCNGG 6 cut(s) 612, 629, 688, 948, 1513, 2607
BstSFI CTRYAG 9 cut(s) 340, 624, 677, 1214, 1929, 2004, 2633, 2654, 2787
BstSLI GKGCMC 2 cut(s) 68, 302
BstUI CGCG 5 cut(s) 357, 551, 880, 999, 1001
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 2 cut(s) 408, 1840
BstXI CCANNNNNNTGG 2 cut(s) 466, 2615
BstYI RGATCY 2 cut(s) 408, 1840
BstZ17I GTATAC 1 cut(s) 2759
Bsu36I CCTNAGG 2 cut(s) 870, 1644
BsuI GTATCC 1 cut(s) 510
BsuRI GGCC 9 cut(s) 279, 635, 656, 820, 857, 1205, 1829, 2288, 2607
BtgI CCRYGG 5 cut(s) 309, 858, 1727, 2370, 2395
BtsI GCAGTG 3 cut(s) 683, 1602, 2364
BtsIMutI CAGTG 6 cut(s) 683, 1416, 1485, 1602, 1881, 2364
BveI ACCTGC 1 cut(s) 369
CaiI CAGNNNCTG 4 cut(s) 652, 685, 973, 1282
CciI TCATGA 3 cut(s) 778, 1344, 2481
CfoI GCGC 8 cut(s) 26, 32, 130, 485, 999, 1001, 1003, 1375
Cfr10I RCCGGY 3 cut(s) 26, 32, 934
Cfr13I GGNCC 5 cut(s) 423, 610, 633, 1828, 2459
CseI GACGC 5 cut(s) 152, 445, 864, 1038, 2319
Csp6I GTAC 7 cut(s) 133, 253, 769, 1393, 1510, 2021, 2564
CviQI GTAC 7 cut(s) 133, 253, 769, 1393, 1510, 2021, 2564
DinI GGCGCC 2 cut(s) 25, 31
DraIII CACNNNGTG 2 cut(s) 160, 1532
DrdI GACNNNNNNGTC 1 cut(s) 2457
DseDI GACNNNNNNGTC 1 cut(s) 2457
EaeI YGGCCR 4 cut(s) 277, 654, 855, 2605
Eam1104I CTCTTC 3 cut(s) 446, 1179, 1589
EarI CTCTTC 3 cut(s) 446, 1179, 1589
EciI GGCGGA 2 cut(s) 119, 821
Ecl136II GAGCTC 2 cut(s) 1110, 1867
Eco130I CCWWGG 4 cut(s) 1053, 1727, 2016, 2370
Eco147I AGGCCT 1 cut(s) 1205
Eco24I GRGCYC 4 cut(s) 1043, 1112, 1869, 1989
Eco32I GATATC 1 cut(s) 2404
Eco47I GGWCC 3 cut(s) 423, 610, 2459
Eco47III AGCGCT 1 cut(s) 1374
Eco53kI GAGCTC 2 cut(s) 1110, 1867
Eco57I CTGAAG 3 cut(s) 1440, 2067, 2820
Eco81I CCTNAGG 2 cut(s) 870, 1644
Eco88I CYCGRG 2 cut(s) 395, 486
EcoICRI GAGCTC 2 cut(s) 1110, 1867
EcoO109I RGGNCCY 1 cut(s) 610
EcoRI GAATTC 1 cut(s) 2714
EcoRII CCWGG 3 cut(s) 948, 1513, 2607
EcoRV GATATC 1 cut(s) 2404
EcoT14I CCWWGG 4 cut(s) 1053, 1727, 2016, 2370
EcoT22I ATGCAT 1 cut(s) 1674
EcoT38I GRGCYC 4 cut(s) 1043, 1112, 1869, 1989
EgeI GGCGCC 2 cut(s) 25, 31
EheI GGCGCC 2 cut(s) 25, 31
ErhI CCWWGG 4 cut(s) 1053, 1727, 2016, 2370
FalI AAGNNNNNCTT 2 cut(s) 2801, 2833
FaqI GGGAC 5 cut(s) 436, 1448, 1589, 1745, 2286
FauI CCCGC 3 cut(s) 362, 396, 2325
FauNDI CATATG 1 cut(s) 2726
FblI GTMKAC 3 cut(s) 141, 2449, 2758
FriOI GRGCYC 4 cut(s) 1043, 1112, 1869, 1989
FspBI CTAG 7 cut(s) 600, 1338, 2178, 2643, 2703, 2826, 2880
GlaI GCGC 8 cut(s) 25, 31, 129, 484, 998, 1000, 1002, 1374
GsaI CCCAGC 3 cut(s) 43, 1509, 1834
GsuI CTGGAG 2 cut(s) 488, 1536
HaeII RGCGCY 4 cut(s) 27, 33, 486, 1376
HaeIII GGCC 9 cut(s) 279, 635, 656, 820, 857, 1205, 1829, 2288, 2607
HgaI GACGC 5 cut(s) 152, 445, 864, 1038, 2319
HhaI GCGC 8 cut(s) 26, 32, 130, 485, 999, 1001, 1003, 1375
Hin1I GRCGYC 4 cut(s) 24, 30, 429, 875
Hin6I GCGC 8 cut(s) 24, 30, 128, 483, 997, 999, 1001, 1373
HinP1I GCGC 8 cut(s) 24, 30, 128, 483, 997, 999, 1001, 1373
HincII GTYRAC 3 cut(s) 142, 2450, 2796
HindII GTYRAC 3 cut(s) 142, 2450, 2796
HindIII AAGCTT 1 cut(s) 704
HphI GGTGA 7 cut(s) 124, 916, 1402, 1544, 1627, 2288, 2605
Hpy99I CGWCG 3 cut(s) 146, 431, 880
HpyAV CCTTC 5 cut(s) 40, 334, 459, 466, 950
HpyCH4III ACNGT 5 cut(s) 139, 721, 1218, 1493, 2056
HpyCH4IV ACGT 3 cut(s) 429, 1678, 2868
HpySE526I ACGT 3 cut(s) 429, 1678, 2868
Hsp92I GRCGYC 4 cut(s) 24, 30, 429, 875
HspAI GCGC 8 cut(s) 24, 30, 128, 483, 997, 999, 1001, 1373
KasI GGCGCC 2 cut(s) 23, 29
KpnI GGTACC 1 cut(s) 1513
KroI GCCGGC 2 cut(s) 26, 32
KroNI GCCGGC 2 cut(s) 28, 34
LguI GCTCTTC 1 cut(s) 446
LweI GCATC 9 cut(s) 1170, 1492, 1525, 1645, 1659, 1681, 1738, 1783, 2455
MaeI CTAG 7 cut(s) 600, 1338, 2178, 2643, 2703, 2826, 2880
MaeII ACGT 3 cut(s) 429, 1678, 2868
MaeIII GTNAC 5 cut(s) 681, 1304, 1408, 1819, 2230
MfeI CAATTG 1 cut(s) 1401
MflI RGATCY 2 cut(s) 408, 1840
MhlI GDGCHC 8 cut(s) 68, 302, 396, 792, 1043, 1112, 1869, 1989
MluCI AATT 9 cut(s) 73, 510, 1229, 1401, 1767, 2258, 2477, 2714, 2847
Mly113I GGCGCC 2 cut(s) 24, 30
MlyI GAGTC 7 cut(s) 116, 185, 326, 1267, 1273, 1775, 2445
MmeI TCCRAC 2 cut(s) 964, 1233
Mph1103I ATGCAT 1 cut(s) 1674
MreI CGCCGGCG 2 cut(s) 26, 32
MroNI GCCGGC 2 cut(s) 26, 32
MroXI GAANNNNTTC 1 cut(s) 2714
MseI TTAA 9 cut(s) 702, 1074, 1481, 1689, 1710, 1788, 1811, 2166, 2750
MslI CAYNNNNRTG 4 cut(s) 722, 783, 2369, 2705
MspA1I CMGCKG 1 cut(s) 676
MspR9I CCNGG 6 cut(s) 614, 631, 690, 950, 1515, 2609
MunI CAATTG 1 cut(s) 1401
Mva1269I GAATGC 1 cut(s) 1966
MvaI CCWGG 3 cut(s) 950, 1515, 2609
MvnI CGCG 5 cut(s) 357, 551, 880, 999, 1001
NaeI GCCGGC 2 cut(s) 28, 34
NarI GGCGCC 2 cut(s) 24, 30
NciI CCSGG 3 cut(s) 614, 631, 690
NcoI CCATGG 2 cut(s) 1727, 2370
NdeI CATATG 1 cut(s) 2726
NgoMIV GCCGGC 2 cut(s) 26, 32
NheI GCTAGC 1 cut(s) 2879
NmeAIII GCCGAG 1 cut(s) 127
NmuCI GTSAC 3 cut(s) 681, 1304, 1408
NsiI ATGCAT 1 cut(s) 1674
NspI RCATGY 2 cut(s) 1265, 2349
OliI CACNNNNGTG 1 cut(s) 722
PaeR7I CTCGAG 1 cut(s) 395
PagI TCATGA 3 cut(s) 778, 1344, 2481
PauI GCGCGC 2 cut(s) 997, 999
PceI AGGCCT 1 cut(s) 1205
PciI ACATGT 2 cut(s) 1261, 2345
PciSI GCTCTTC 1 cut(s) 446
PcsI WCGNNNNNNNCGW 3 cut(s) 162, 171, 426
PctI GAATGC 1 cut(s) 1966
PdiI GCCGGC 2 cut(s) 28, 34
PdmI GAANNNNTTC 1 cut(s) 2714
PfeI GAWTC 4 cut(s) 224, 1348, 2042, 2638
PflMI CCANNNNNTGG 1 cut(s) 1027
PfoI TCCNGGA 1 cut(s) 948
PleI GAGTC 7 cut(s) 115, 184, 325, 1267, 1273, 1775, 2445
PluTI GGCGCC 2 cut(s) 27, 33
PpsI GAGTC 7 cut(s) 115, 184, 325, 1267, 1273, 1775, 2445
PpuMI RGGWCCY 1 cut(s) 610
PscI ACATGT 2 cut(s) 1261, 2345
PshAI GACNNNNGTC 1 cut(s) 428
PshBI ATTAAT 1 cut(s) 1074
PsiI TTATAA 1 cut(s) 2861
Psp124BI GAGCTC 2 cut(s) 1112, 1869
Psp5II RGGWCCY 1 cut(s) 610
Psp6I CCWGG 3 cut(s) 948, 1513, 2607
PspFI CCCAGC 3 cut(s) 39, 1505, 1830
PspGI CCWGG 3 cut(s) 948, 1513, 2607
PspPI GGNCC 5 cut(s) 423, 610, 633, 1828, 2459
PspPPI RGGWCCY 1 cut(s) 610
PstI CTGCAG 3 cut(s) 681, 2637, 2658
PstNI CAGNNNCTG 4 cut(s) 652, 685, 973, 1282
PsuI RGATCY 2 cut(s) 408, 1840
PteI GCGCGC 2 cut(s) 997, 999
PvuII CAGCTG 1 cut(s) 676
RsaI GTAC 7 cut(s) 134, 254, 770, 1394, 1511, 2022, 2565
RsaNI GTAC 7 cut(s) 133, 253, 769, 1393, 1510, 2021, 2564
RseI CAYNNNNRTG 4 cut(s) 722, 783, 2369, 2705
SacI GAGCTC 2 cut(s) 1112, 1869
SalI GTCGAC 2 cut(s) 140, 2448
SapI GCTCTTC 1 cut(s) 446
SaqAI TTAA 9 cut(s) 702, 1074, 1481, 1689, 1710, 1788, 1811, 2166, 2750
Sau96I GGNCC 5 cut(s) 423, 610, 633, 1828, 2459
ScaI AGTACT 1 cut(s) 1394
SchI GAGTC 7 cut(s) 116, 185, 326, 1267, 1273, 1775, 2445
ScrFI CCNGG 6 cut(s) 614, 631, 690, 950, 1515, 2609
SduI GDGCHC 8 cut(s) 68, 302, 396, 792, 1043, 1112, 1869, 1989
SfaNI GCATC 9 cut(s) 1170, 1492, 1525, 1645, 1659, 1681, 1738, 1783, 2455
SfcI CTRYAG 9 cut(s) 340, 624, 677, 1214, 1929, 2004, 2633, 2654, 2787
SfoI GGCGCC 2 cut(s) 25, 31
Sfr274I CTCGAG 1 cut(s) 395
SgrAI CRCCGGYG 2 cut(s) 26, 32
SinI GGWCC 3 cut(s) 423, 610, 2459
SlaI CTCGAG 1 cut(s) 395
SmiMI CAYNNNNRTG 4 cut(s) 722, 783, 2369, 2705
SmlI CTYRAG 2 cut(s) 395, 2453
SmoI CTYRAG 2 cut(s) 395, 2453
SpeI ACTAGT 1 cut(s) 2825
Sse9I AATT 9 cut(s) 73, 510, 1229, 1401, 1767, 2258, 2477, 2714, 2847
SseBI AGGCCT 1 cut(s) 1205
SspDI GGCGCC 2 cut(s) 23, 29
SspMI CTAG 7 cut(s) 600, 1338, 2178, 2643, 2703, 2826, 2880
SstI GAGCTC 2 cut(s) 1112, 1869
StuI AGGCCT 1 cut(s) 1205
StyD4I CCNGG 6 cut(s) 612, 629, 688, 948, 1513, 2607
StyI CCWWGG 4 cut(s) 1053, 1727, 2016, 2370
TaaI ACNGT 5 cut(s) 139, 721, 1218, 1493, 2056
TaiI ACGT 3 cut(s) 432, 1681, 2871
TaqI TCGA 9 cut(s) 141, 165, 192, 366, 396, 524, 1112, 1472, 2449
TaqII GACCGA 1 cut(s) 440
TasI AATT 9 cut(s) 73, 510, 1229, 1401, 1767, 2258, 2477, 2714, 2847
TatI WGTACW 4 cut(s) 132, 768, 1392, 2563
TauI GCSGC 3 cut(s) 39, 279, 1006
TfiI GAWTC 4 cut(s) 224, 1348, 2042, 2638
Tru1I TTAA 9 cut(s) 702, 1074, 1481, 1689, 1710, 1788, 1811, 2166, 2750
Tru9I TTAA 9 cut(s) 702, 1074, 1481, 1689, 1710, 1788, 1811, 2166, 2750
TscAI CASTG 6 cut(s) 690, 1423, 1492, 1609, 1881, 2371
TseFI GTSAC 3 cut(s) 681, 1304, 1408
Tsp45I GTSAC 3 cut(s) 681, 1304, 1408
TspGWI ACGGA 3 cut(s) 875, 1120, 2534
TspRI CASTG 6 cut(s) 690, 1423, 1492, 1609, 1881, 2371
Van91I CCANNNNNTGG 1 cut(s) 1027
VneI GTGCAC 1 cut(s) 298
VpaK11BI GGWCC 3 cut(s) 423, 610, 2459
VspI ATTAAT 1 cut(s) 1074
XapI RAATTY 4 cut(s) 2258, 2477, 2714, 2847
XceI RCATGY 2 cut(s) 1265, 2349
XcmI CCANNNNNNNNNTGG 1 cut(s) 1051
XhoI CTCGAG 1 cut(s) 395
XmiI GTMKAC 3 cut(s) 141, 2449, 2758
XmnI GAANNNNTTC 1 cut(s) 2714
XspI CTAG 7 cut(s) 600, 1338, 2178, 2643, 2703, 2826, 2880
ZraI GACGTC 1 cut(s) 430
ZrmI AGTACT 1 cut(s) 1394
Zsp2I ATGCAT 1 cut(s) 1674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.