MD16G1153800.v1.1

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009857
Physical Location & Seq
Reverse (-)
12204544 .. 12211349
6806 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1153800.v1.1.491

Sequence Viewer

Length: 3363 bp
ATGGCGTCAGGAGCTCAGTCGTCGGGCACGAGCAATATCAAGGCTCACCACAACACGGAGTCTGTGAGCAAAGCCATTGCTCAGTACACTGTAGATGCTCGGCTGCACGCCGTGTTCGAGCAGTCCGGGGAGTCCGGCAAGTCGTTCGACTACTCGCAGAGCATGAAAACCACCAAAGATTCCGTCCCGGAGCAGCAGATTACGGCGTACCTGTCGAAGATTCAGAGGGGCGGCCATGTCCAACCTTTCGGGTGCATGATGGCCGTGGACGAAGCCACGTTCGGAGTCATTGCGTATAGCGAGAACGCACGCGACATGCTCGACCTAACGCCGCAGTCAGTGCCGATCCTTGAAAAGCCGGAGATTCTCACAATTGGGACCGACGTCCGTACGCTATTCACACCGTCGAGCGCGGTGTTGCTGGAGAAGGCATTTGGGGCTCGGGAGATAACCCTTTTGAACCCGATTTGGATCCACTCTAAGATTTCTGGAAAGCCCTTTTACGCAATTTTGCATAGGATTGATGTTGGGGTCGTGATTGATTTGGAGCCTGCGAGAACAGAAGACCCTGCGCTGTCGATTGCCGGCGCGGTGCAGTCGCAGAAGCTGGCGGTGAGGGCGATTTCGCAGCTGCAGTCGCTGCCGGGCGGCGACATTAAGCTTTTGTGTGACACTGTGGTAGAGAGTGTGAGGGAGCTTACTGGCTATGATAGAGTTATGGTTTATAAGTTTCACGAGGATGAGCATGGTGAGGTTGTGGCTGAGAGTAAAAGGCCTGACTTGGACCCATACCTTGGGCTGCACTACCCGGCCACGGATATACCACAGGCGTCAAGGTTCTTGTTCAAGCAGAACCGGGCTCGAATGATAGTAGATTGTCACGCCAAGCCGGTTCATGTGATTCAGGATGAAGGGCTGATGCAGCCTTTGTGCTTGGTTGGATCCACACTAAGAGCCCCACATGGTTGCCATTCCCAGTACATGGCTAATATGGGATCCATTGCGTCATTGGCGTTGGCGGTAATCATCAATGGAAACGACGAGGAAGCTCTTGGTGGGAGAAATTCAATGAGATTATGGGGCCTGGTTGTTTGCCATCACACCTCTGCTCGGTGCATTCCATTTCCGCTTCGGTATGCTTGTGAGTTTTTAATGCAGGCCTTTGGACTTCAATTGAATATGGAATTACAATTGGCTTCACAAATGTCTGAGAAACATGTTTTAAGGACACAGACTCTGTTGTGTGATATGCTTCTGCGTGATACCCCAACTGGCATTGTTACTCAAAGTCCTAGTATAATGAACCTTGTGAAATGTGATGGGGCTGCACTCTACTACCAAGGGAACTACTACCCTCTTGGTGTGACGCCCACCGAAGCCCAGATAAAGGACATTGTGGAGTGGTTGTTGGCTTCCCATGGAAGTTCAACTGGTTTGAGTACAGATAGTTTGGCTGATGCCGGGTACCCTGGAGCTGCCTCTCTTGGTGATGCAGTTTGTGGAATGGCGGCTGCTTATATTACTAAAAGGGATTTTCTGTTCTGGTTCCGATCCCACACTGGGAAAGAGATCAAATGGGGTGGAGCAAAGCATCATCCAGAGGACAAGGATGATGGGCAGAGGATGCATCCACGCTCTTCATTCAAAGCGTTTTTGGAAGTGGTTAAAAGCCGGAGCTTGCCATGGGAGAATGCAGAAATGGATGCAATACACTCTTTGCAGATTATTTTGCGTGACTCATTTAAGAACACAGAGACAAACAATACAAATGCTGTTATGCGGGCCCAGCTTGGCGATCTGGAGTTTCAAGGGATCAATGAGCTCAGCTCCGTAGCAAGAGAAATGGTTAGGTTGATAGAGACTGCAACTGCTCCCATACTTGCTGTCGATGTTAATGGCTGTATAAATGGGTGGAATGCAAAGGTTGCAGAGTTGACCGGTCTCTCAGTTGAGGAAGCTACCGGGAAGTCCTTGGTTCACGATCTCATTTACAAAGAATCTGAAGAAATTGTTGAAAAACTTCTAACCCGCGCTTTAAAAGGTGAAGAAGATAAGAATGTCGAAATCAAAATGAGGACATTTGGCCCAGAGCATGATAACAAGCCTGTCTTCATAGTGGTTAATGCTTGCTCTAGCAAGGATTACGCTAATAACATGGTTGGAGTTTGCTTTGTTGGTCAGGACGTTACTGGTCAAAAAGTAATAATGGACAAATTCATAAAAATACAAGGTGATTACAAAGCCATTGTTCATAGCCCCAATCCTTTGATCCCTCCCATATTTGCTTCAGATGATAACACATGTTGCTCGGAATGGAACACTGCCATGGAAAAGCTCACTGGGTGGAACCAGGGAGAAATCCTTGGAAAAATGTTGGTTGGAGAGGTCTTCGGCAGTTGCTGTCGAATCAAGGGTCCAGATGCTATGACAAAATTCATGATTGTCTTGCACAATGCCATTGGAGGGCTAGACACAGACAAATTCCCCTTTTCGTTCTTTGACCGGAATGGGAAATATGTACAAGCTCTCTTGACAGCAAATAAGAGGGTGGATACAGAAGGTCAGGTTATTGGAGCTTTCTGCTTTTTGCAGATTGCTAGTCCGGAACTGCAGCAAACTCTTAAAGTACAGAAGCAACAAGAAAATGAATGTTTATCTAGGATGAAAGAATTGGCTTACATTTGCCAGGAAGTAAAAAATCCTTTAAGTGGTATACGCTTTACTAACTCACTTTTGGAGGCTACGGACTTAACTGAAGACCAAAAGCAGTTTCTGGAGACTAGTGCTGCTTGTGAGAAGCAAATTTTGAAGATTATAAAAGATGTTGATCTGGATAGCATTGACGATGGATCACTGGAGCTTGAGAAGACAGGATTCTTACTTGGGAGCGTTATAAACGCTGTTGTTAGCCAAGTAATGTTATTGCTCAGAGAAAGAGATCTACAATTGATTCGAGATATTCCTGAAGAAATCAAAACATTGGCCGTCTATGGTGATCAAGTGAGAATTCAACAGGTCTTGGCTGATTTCTTATTGAATATGGTACGTTATGCACCCTCTCCTGAAGGCTGGGTGGAGATTCATGTTCTTCCAAGCTTGAAGAAAGTACCGGATGGAATCACTCTGGTTCATACTGAATTCAGGTTGGCATGTCCTGGCGAAGGTCTCCCTCCTCAATTAGTTCAAGACATGTTCCATAGCAGTCAATGGATGACTCAGGAAGGTCTTGGACTGAGCATGTGCACGAAGATTTTAAAGCTCATGAACGGTGAAGTCCAATATGTCAGAGAGTCAGAAAGATGTTATTTCTTAATTACTCTTGAGCTTCCTATGCCTCGGAGACCTACAAATAGTATTGACTAG

Protein Analysis

1121

Amino Acids

124.03

Weight (kDa)

5.5

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 69 - 185 1.2e-37 PAS fold
GAF PF01590 219 - 396 3.7e-16 GAF domain
PHY PF00360 409 - 584 5.7e-57 Phytochrome region
PAS PF00989 614 - 729 5.7e-24 PAS fold
PAS_4 PF08448 622 - 732 1.5e-06 PAS fold
PAS PF00989 745 - 864 1.9e-19 PAS fold
HisKA PF00512 888 - 950 9.6e-13 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 998 - 1109 6.1e-13 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 726, 2816, 2894
AatII GACGTC 1 cut(s) 387
Acc65I GGTACC 1 cut(s) 1464
AccB1I GGYRCC 1 cut(s) 1464
AccB7I CCANNNNNTGG 2 cut(s) 794, 982
AccI GTMKAC 1 cut(s) 2713
AccII CGCG 4 cut(s) 312, 413, 590, 2031
AccIII TCCGGA 1 cut(s) 2602
AcoI YGGCCR 4 cut(s) 232, 261, 810, 2982
AcsI RAATTY 7 cut(s) 1063, 2213, 2432, 2480, 2802, 3006, 3137
AcuI CTGAAG 5 cut(s) 2022, 2271, 2775, 2985, 3084
AcyI GRCGYC 4 cut(s) 5, 384, 830, 1367
AdeI CACNNNGTG 2 cut(s) 112, 2343
AflIII ACRYGT 3 cut(s) 1216, 2300, 3189
AgeI ACCGGT 1 cut(s) 1937
AhlI ACTAGT 1 cut(s) 2780
AjnI CCWGG 5 cut(s) 1083, 1468, 2349, 2685, 3154
AjuI GAANNNNNNNTTGG 2 cut(s) 2754, 2786
AloI GAACNNNNNNTCC 2 cut(s) 1523, 1555
Alw21I GWGCWC 3 cut(s) 16, 1824, 3245
Alw26I GTCTC 6 cut(s) 1748, 1853, 1946, 2771, 3170, 3334
Alw44I GTGCAC 1 cut(s) 3241
AlwNI CAGNNNCTG 5 cut(s) 607, 640, 1237, 2400, 2773
Ama87I CYCGRG 1 cut(s) 441
Aor13HI TCCGGA 1 cut(s) 2602
AoxI GGCC 9 cut(s) 232, 261, 773, 810, 1081, 1158, 1782, 2083, 2982
ApaI GGGCCC 1 cut(s) 1786
ApaLI GTGCAC 1 cut(s) 3241
ApoI RAATTY 7 cut(s) 1063, 2213, 2432, 2480, 2802, 3006, 3137
ArsI GACNNNNNNTTYG 4 cut(s) 168, 200, 2398, 2430
AsiGI ACCGGT 1 cut(s) 1937
Asp700I GAANNNNTTC 1 cut(s) 2019
Asp718I GGTACC 1 cut(s) 1464
AspLEI GCGC 4 cut(s) 413, 574, 590, 2033
AspS9I GGNCC 7 cut(s) 378, 784, 1081, 1782, 1783, 2084, 2414
AsuC2I CCSGG 7 cut(s) 127, 188, 645, 809, 857, 1462, 1963
AsuHPI GGTGA 8 cut(s) 38, 625, 761, 1499, 2054, 2243, 3005, 3281
AvaI CYCGRG 1 cut(s) 441
AvaII GGWCC 3 cut(s) 378, 784, 2414
BaeGI GKGCMC 3 cut(s) 29, 1786, 3245
BamHI GGATCC 3 cut(s) 471, 941, 995
BanI GGYRCC 1 cut(s) 1464
BanII GRGCYC 6 cut(s) 16, 442, 862, 958, 1786, 1824
BauI CACGAG 2 cut(s) 28, 734
BbsI GAAGAC 5 cut(s) 570, 2101, 2380, 2763, 2873
Bbv12I GWGCWC 3 cut(s) 16, 1824, 3245
BccI CCATC 6 cut(s) 253, 1104, 1313, 1607, 2840, 3107
BceAI ACGGC 4 cut(s) 95, 219, 248, 2969
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 5 cut(s) 1085, 1470, 2351, 2687, 3156
BciVI GTATCC 1 cut(s) 2545
BclI TGATCA 1 cut(s) 2995
BcnI CCSGG 7 cut(s) 127, 188, 645, 809, 857, 1462, 1963
BcoDI GTCTC 6 cut(s) 1748, 1853, 1946, 2771, 3170, 3334
BcuI ACTAGT 1 cut(s) 2780
BfaI CTAG 7 cut(s) 1293, 2133, 2468, 2598, 2658, 2781, 3361
BfmI CTRYAG 3 cut(s) 90, 632, 2609
BfuI GTATCC 1 cut(s) 2545
BglII AGATCT 1 cut(s) 2938
BlpI GCTNAGC 1 cut(s) 1823
Bme18I GGWCC 3 cut(s) 378, 784, 2414
BmeT110I CYCGRG 1 cut(s) 441
BmgT120I GGNCC 7 cut(s) 378, 784, 1081, 1782, 1783, 2084, 2414
BmrI ACTGGG 3 cut(s) 970, 1569, 2349
BmsI GCATC 9 cut(s) 85, 909, 1447, 1480, 1600, 1614, 1636, 1693, 2410
BmuI ACTGGG 3 cut(s) 970, 1569, 2349
BoxI GACNNNNGTC 1 cut(s) 383
BpiI GAAGAC 5 cut(s) 570, 2101, 2380, 2763, 2873
BplI GAGNNNNNCTC 2 cut(s) 1811, 1843
BpmI CTGGAG 5 cut(s) 443, 1491, 1820, 2795, 2876
Bpu1102I GCTNAGC 1 cut(s) 1823
BpuEI CTTGAG 2 cut(s) 2882, 3341
BpuMI CCSGG 7 cut(s) 127, 188, 645, 809, 857, 1462, 1963
BsaBI GATNNNNATC 2 cut(s) 470, 2826
BsaHI GRCGYC 4 cut(s) 5, 384, 830, 1367
BsaI GGTCTC 3 cut(s) 1946, 3170, 3334
BsaWI WCCGGW 4 cut(s) 1937, 2502, 2602, 3109
BsaXI ACNNNNNCTCC 2 cut(s) 1391, 1421
Bse118I RCCGGY 3 cut(s) 584, 889, 1937
Bse1I ACTGG 8 cut(s) 706, 976, 1276, 1435, 1564, 2194, 2344, 2859
Bse3DI GCAATG 3 cut(s) 75, 288, 999
Bse8I GATNNNNATC 2 cut(s) 470, 2826
BseAI TCCGGA 1 cut(s) 2602
BseBI CCWGG 5 cut(s) 1085, 1470, 2351, 2687, 3156
BseJI GATNNNNATC 2 cut(s) 470, 2826
BseMI GCAATG 3 cut(s) 75, 288, 999
BseMII CTCAG 9 cut(s) 29, 95, 753, 1200, 1837, 1959, 2941, 3224, 3230
BseNI ACTGG 8 cut(s) 706, 976, 1276, 1435, 1564, 2194, 2344, 2859
BseRI GAGGAG 1 cut(s) 3162
BseSI GKGCMC 3 cut(s) 29, 1786, 3245
BseYI CCCAGC 2 cut(s) 1785, 3069
BsgI GTGCAG 4 cut(s) 89, 614, 785, 1311
Bsh1236I CGCG 4 cut(s) 312, 413, 590, 2031
BshFI GGCC 9 cut(s) 234, 263, 775, 812, 1083, 1160, 1784, 2085, 2984
BshNI GGYRCC 1 cut(s) 1464
BshTI ACCGGT 1 cut(s) 1937
BsiHKAI GWGCWC 3 cut(s) 16, 1824, 3245
BsiHKCI CYCGRG 1 cut(s) 441
BsiWI CGTACG 1 cut(s) 389
BslFI GGGAC 2 cut(s) 170, 391
BsmAI GTCTC 6 cut(s) 1748, 1853, 1946, 2771, 3170, 3334
BsmFI GGGAC 2 cut(s) 170, 391
BsmI GAATGC 3 cut(s) 1116, 1696, 1921
BsnI GGCC 9 cut(s) 234, 263, 775, 812, 1083, 1160, 1784, 2085, 2984
Bso31I GGTCTC 3 cut(s) 1946, 3170, 3334
BsoBI CYCGRG 1 cut(s) 441
Bsp120I GGGCCC 1 cut(s) 1782
Bsp1286I GDGCHC 8 cut(s) 16, 29, 442, 862, 958, 1786, 1824, 3245
Bsp13I TCCGGA 1 cut(s) 2602
Bsp1407I TGTACA 1 cut(s) 2518
Bsp1720I GCTNAGC 1 cut(s) 1823
Bsp19I CCATGG 3 cut(s) 1417, 1682, 2325
BspANI GGCC 9 cut(s) 234, 263, 775, 812, 1083, 1160, 1784, 2085, 2984
BspCNI CTCAG 9 cut(s) 28, 94, 754, 1201, 1836, 1958, 2940, 3225, 3229
BspEI TCCGGA 1 cut(s) 2602
BspFNI CGCG 4 cut(s) 312, 413, 590, 2031
BspHI TCATGA 2 cut(s) 2436, 3261
BspMAI CTGCAG 2 cut(s) 636, 2613
BspQI GCTCTTC 1 cut(s) 1642
BspT107I GGYRCC 1 cut(s) 1464
BspTNI GGTCTC 3 cut(s) 1946, 3170, 3334
BsrDI GCAATG 3 cut(s) 75, 288, 999
BsrFI RCCGGY 3 cut(s) 584, 889, 1937
BsrGI TGTACA 1 cut(s) 2518
BsrI ACTGG 8 cut(s) 706, 976, 1276, 1435, 1564, 2194, 2344, 2859
BssAI RCCGGY 3 cut(s) 584, 889, 1937
BssNAI GTATAC 1 cut(s) 2714
BssNI GRCGYC 4 cut(s) 5, 384, 830, 1367
BssSI CACGAG 2 cut(s) 28, 734
BssT1I CCWWGG 7 cut(s) 793, 1339, 1417, 1682, 1971, 2325, 2362
Bst1107I GTATAC 1 cut(s) 2714
Bst2BI CACGAG 2 cut(s) 28, 734
Bst2UI CCWGG 5 cut(s) 1085, 1470, 2351, 2687, 3156
Bst4CI ACNGT 4 cut(s) 91, 405, 676, 3269
Bst6I CTCTTC 1 cut(s) 1642
BstACI GRCGYC 4 cut(s) 5, 384, 830, 1367
BstAPI GCANNNNNTGC 4 cut(s) 340, 640, 1624, 1925
BstAUI TGTACA 1 cut(s) 2518
BstC8I GCNNGC 9 cut(s) 108, 310, 552, 586, 609, 1158, 1679, 1782, 2128
BstDSI CCRYGG 5 cut(s) 264, 813, 1417, 1682, 2325
BstENI CCTNNNNNAGG 1 cut(s) 3159
BstFNI CGCG 4 cut(s) 312, 413, 590, 2031
BstHHI GCGC 4 cut(s) 413, 574, 590, 2033
BstMAI GTCTC 6 cut(s) 1748, 1853, 1946, 2771, 3170, 3334
BstNI CCWGG 5 cut(s) 1085, 1470, 2351, 2687, 3156
BstNSI RCATGY 6 cut(s) 319, 1220, 2304, 3153, 3193, 3241
BstPAI GACNNNNGTC 1 cut(s) 383
BstSFI CTRYAG 3 cut(s) 90, 632, 2609
BstSLI GKGCMC 3 cut(s) 29, 1786, 3245
BstUI CGCG 4 cut(s) 312, 413, 590, 2031
BstV2I GAAGAC 5 cut(s) 570, 2101, 2380, 2763, 2873
BstX2I RGATCY 4 cut(s) 471, 941, 995, 2938
BstYI RGATCY 4 cut(s) 471, 941, 995, 2938
BstZ17I GTATAC 1 cut(s) 2714
BsuI GTATCC 1 cut(s) 2545
BsuRI GGCC 9 cut(s) 234, 263, 775, 812, 1083, 1160, 1784, 2085, 2984
BtgI CCRYGG 5 cut(s) 264, 813, 1417, 1682, 2325
BtsI GCAGTG 1 cut(s) 2319
BtsIMutI CAGTG 7 cut(s) 87, 345, 672, 1557, 2319, 2337, 2852
Cac8I GCNNGC 9 cut(s) 108, 310, 552, 586, 609, 1158, 1679, 1782, 2128
CaiI CAGNNNCTG 5 cut(s) 607, 640, 1237, 2400, 2773
CciI TCATGA 2 cut(s) 2436, 3261
CfoI GCGC 4 cut(s) 413, 574, 590, 2033
Cfr10I RCCGGY 3 cut(s) 584, 889, 1937
Cfr13I GGNCC 7 cut(s) 378, 784, 1081, 1782, 1783, 2084, 2414
CseI GACGC 3 cut(s) 819, 993, 1375
CspAI ACCGGT 1 cut(s) 1937
CspCI CAANNNNNGTGG 2 cut(s) 1561, 1596
DraI TTTAAA 2 cut(s) 2037, 3255
DraIII CACNNNGTG 2 cut(s) 112, 2343
EaeI YGGCCR 4 cut(s) 232, 261, 810, 2982
Eam1104I CTCTTC 1 cut(s) 1642
EarI CTCTTC 1 cut(s) 1642
Ecl136II GAGCTC 2 cut(s) 14, 1822
Eco130I CCWWGG 7 cut(s) 793, 1339, 1417, 1682, 1971, 2325, 2362
Eco147I AGGCCT 2 cut(s) 775, 1160
Eco24I GRGCYC 6 cut(s) 16, 442, 862, 958, 1786, 1824
Eco31I GGTCTC 3 cut(s) 1946, 3170, 3334
Eco47I GGWCC 3 cut(s) 378, 784, 2414
Eco53kI GAGCTC 2 cut(s) 14, 1822
Eco57I CTGAAG 5 cut(s) 2022, 2271, 2775, 2985, 3084
Eco88I CYCGRG 1 cut(s) 441
EcoICRI GAGCTC 2 cut(s) 14, 1822
EcoNI CCTNNNNNAGG 1 cut(s) 3159
EcoO109I RGGNCCY 1 cut(s) 1081
EcoRI GAATTC 2 cut(s) 3006, 3137
EcoRII CCWGG 5 cut(s) 1083, 1468, 2349, 2685, 3154
EcoT14I CCWWGG 7 cut(s) 793, 1339, 1417, 1682, 1971, 2325, 2362
EcoT22I ATGCAT 1 cut(s) 1629
EcoT38I GRGCYC 6 cut(s) 16, 442, 862, 958, 1786, 1824
ErhI CCWWGG 7 cut(s) 793, 1339, 1417, 1682, 1971, 2325, 2362
FalI AAGNNNNNCTT 2 cut(s) 2093, 2125
FaqI GGGAC 2 cut(s) 170, 391
FauI CCCGC 2 cut(s) 1773, 2036
FbaI TGATCA 1 cut(s) 2995
FblI GTMKAC 1 cut(s) 2713
FriOI GRGCYC 6 cut(s) 16, 442, 862, 958, 1786, 1824
FspBI CTAG 7 cut(s) 1293, 2133, 2468, 2598, 2658, 2781, 3361
GlaI GCGC 4 cut(s) 412, 573, 589, 2032
GsaI CCCAGC 2 cut(s) 1789, 3073
GsuI CTGGAG 5 cut(s) 443, 1491, 1820, 2795, 2876
HaeIII GGCC 9 cut(s) 234, 263, 775, 812, 1083, 1160, 1784, 2085, 2984
HgaI GACGC 3 cut(s) 819, 993, 1375
HhaI GCGC 4 cut(s) 413, 574, 590, 2033
Hin1I GRCGYC 4 cut(s) 5, 384, 830, 1367
Hin6I GCGC 4 cut(s) 411, 572, 588, 2031
HinP1I GCGC 4 cut(s) 411, 572, 588, 2031
HincII GTYRAC 1 cut(s) 1935
HindII GTYRAC 1 cut(s) 1935
HindIII AAGCTT 2 cut(s) 659, 3094
HphI GGTGA 8 cut(s) 38, 625, 761, 1499, 2054, 2243, 3005, 3281
Hpy166II GTNNAC 6 cut(s) 87, 268, 1935, 1978, 2714, 3243
Hpy8I GTNNAC 6 cut(s) 87, 268, 1935, 1978, 2714, 3243
Hpy99I CGWCG 4 cut(s) 25, 386, 409, 1043
HpyAV CCTTC 6 cut(s) 421, 905, 2552, 3059, 3155, 3215
HpyCH4III ACNGT 4 cut(s) 91, 405, 676, 3269
HpyCH4IV ACGT 4 cut(s) 278, 384, 2184, 3046
HpySE526I ACGT 4 cut(s) 278, 384, 2184, 3046
Hsp92I GRCGYC 4 cut(s) 5, 384, 830, 1367
HspAI GCGC 4 cut(s) 411, 572, 588, 2031
Kpn2I TCCGGA 1 cut(s) 2602
KpnI GGTACC 1 cut(s) 1468
KroI GCCGGC 1 cut(s) 584
KroNI GCCGGC 1 cut(s) 586
Ksp22I TGATCA 1 cut(s) 2995
LguI GCTCTTC 1 cut(s) 1642
LweI GCATC 9 cut(s) 85, 909, 1447, 1480, 1600, 1614, 1636, 1693, 2410
MaeI CTAG 7 cut(s) 1293, 2133, 2468, 2598, 2658, 2781, 3361
MaeII ACGT 4 cut(s) 278, 384, 2184, 3046
MaeIII GTNAC 6 cut(s) 668, 878, 1279, 1363, 1733, 2185
MfeI CAATTG 4 cut(s) 372, 1172, 1190, 2945
MflI RGATCY 4 cut(s) 471, 941, 995, 2938
MhlI GDGCHC 8 cut(s) 16, 29, 442, 862, 958, 1786, 1824, 3245
MlyI GAGTC 7 cut(s) 68, 140, 294, 1228, 1730, 3208, 3299
MmeI TCCRAC 4 cut(s) 265, 919, 2140, 2359
Mph1103I ATGCAT 1 cut(s) 1629
MroI TCCGGA 1 cut(s) 2602
MroNI GCCGGC 1 cut(s) 584
MroXI GAANNNNTTC 1 cut(s) 2019
MslI CAYNNNNRTG 2 cut(s) 738, 2324
MspA1I CMGCKG 1 cut(s) 631
MunI CAATTG 4 cut(s) 372, 1172, 1190, 2945
Mva1269I GAATGC 3 cut(s) 1116, 1696, 1921
MvaI CCWGG 5 cut(s) 1085, 1470, 2351, 2687, 3156
MvnI CGCG 4 cut(s) 312, 413, 590, 2031
NaeI GCCGGC 1 cut(s) 586
NciI CCSGG 7 cut(s) 127, 188, 645, 809, 857, 1462, 1963
NcoI CCATGG 3 cut(s) 1417, 1682, 2325
NgoMIV GCCGGC 1 cut(s) 584
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 4 cut(s) 668, 878, 1363, 1733
NsiI ATGCAT 1 cut(s) 1629
NspI RCATGY 6 cut(s) 319, 1220, 2304, 3153, 3193, 3241
PagI TCATGA 2 cut(s) 2436, 3261
PceI AGGCCT 2 cut(s) 775, 1160
PciI ACATGT 3 cut(s) 1216, 2300, 3189
PciSI GCTCTTC 1 cut(s) 1642
PcsI WCGNNNNNNNCGW 2 cut(s) 26, 114
PctI GAATGC 3 cut(s) 1116, 1696, 1921
PdiI GCCGGC 1 cut(s) 586
PdmI GAANNNNTTC 1 cut(s) 2019
Pfl23II CGTACG 1 cut(s) 389
PflMI CCANNNNNTGG 2 cut(s) 794, 982
PfoI TCCNGGA 1 cut(s) 186
PinAI ACCGGT 1 cut(s) 1937
PleI GAGTC 7 cut(s) 67, 139, 293, 1228, 1730, 3208, 3298
PpsI GAGTC 7 cut(s) 67, 139, 293, 1228, 1730, 3208, 3298
PscI ACATGT 3 cut(s) 1216, 2300, 3189
PshAI GACNNNNGTC 1 cut(s) 383
PsiI TTATAA 3 cut(s) 726, 2816, 2894
Psp124BI GAGCTC 2 cut(s) 16, 1824
Psp6I CCWGG 5 cut(s) 1083, 1468, 2349, 2685, 3154
PspFI CCCAGC 2 cut(s) 1785, 3069
PspGI CCWGG 5 cut(s) 1083, 1468, 2349, 2685, 3154
PspLI CGTACG 1 cut(s) 389
PspOMI GGGCCC 1 cut(s) 1782
PspPI GGNCC 7 cut(s) 378, 784, 1081, 1782, 1783, 2084, 2414
PstI CTGCAG 2 cut(s) 636, 2613
PstNI CAGNNNCTG 5 cut(s) 607, 640, 1237, 2400, 2773
PsuI RGATCY 4 cut(s) 471, 941, 995, 2938
PvuII CAGCTG 1 cut(s) 631
RseI CAYNNNNRTG 2 cut(s) 738, 2324
SacI GAGCTC 2 cut(s) 16, 1824
SapI GCTCTTC 1 cut(s) 1642
Sau96I GGNCC 7 cut(s) 378, 784, 1081, 1782, 1783, 2084, 2414
SchI GAGTC 7 cut(s) 68, 140, 294, 1228, 1730, 3208, 3299
SduI GDGCHC 8 cut(s) 16, 29, 442, 862, 958, 1786, 1824, 3245
SfaNI GCATC 9 cut(s) 85, 909, 1447, 1480, 1600, 1614, 1636, 1693, 2410
SfcI CTRYAG 3 cut(s) 90, 632, 2609
SinI GGWCC 3 cut(s) 378, 784, 2414
SmiMI CAYNNNNRTG 2 cut(s) 738, 2324
SmlI CTYRAG 2 cut(s) 2861, 3320
SmoI CTYRAG 2 cut(s) 2861, 3320
SpeI ACTAGT 1 cut(s) 2780
SseBI AGGCCT 2 cut(s) 775, 1160
SspMI CTAG 7 cut(s) 1293, 2133, 2468, 2598, 2658, 2781, 3361
SstI GAGCTC 2 cut(s) 16, 1824
StuI AGGCCT 2 cut(s) 775, 1160
StyI CCWWGG 7 cut(s) 793, 1339, 1417, 1682, 1971, 2325, 2362
TaaI ACNGT 4 cut(s) 91, 405, 676, 3269
TaiI ACGT 4 cut(s) 281, 387, 2187, 3049
TaqII GACCGA 1 cut(s) 395
TatI WGTACW 5 cut(s) 84, 978, 1439, 2518, 2626
TauI GCSGC 4 cut(s) 234, 334, 651, 1511
TscAI CASTG 7 cut(s) 94, 345, 679, 1564, 2326, 2344, 2859
TseFI GTSAC 4 cut(s) 668, 878, 1363, 1733
Tsp45I GTSAC 4 cut(s) 668, 878, 1363, 1733
TspGWI ACGGA 6 cut(s) 71, 172, 377, 830, 1819, 2759
TspRI CASTG 7 cut(s) 94, 345, 679, 1564, 2326, 2344, 2859
Van91I CCANNNNNTGG 2 cut(s) 794, 982
VneI GTGCAC 1 cut(s) 3241
VpaK11BI GGWCC 3 cut(s) 378, 784, 2414
XagI CCTNNNNNAGG 1 cut(s) 3159
XapI RAATTY 7 cut(s) 1063, 2213, 2432, 2480, 2802, 3006, 3137
XceI RCATGY 6 cut(s) 319, 1220, 2304, 3153, 3193, 3241
XcmI CCANNNNNNNNNTGG 1 cut(s) 1006
XmiI GTMKAC 1 cut(s) 2713
XmnI GAANNNNTTC 1 cut(s) 2019
XspI CTAG 7 cut(s) 1293, 2133, 2468, 2598, 2658, 2781, 3361
ZraI GACGTC 1 cut(s) 385
Zsp2I ATGCAT 1 cut(s) 1629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.