RchiOBHm_Chr4g0425301

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009857
Physical Location & Seq
Forward (+)
50908988 .. 50914514
5527 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39446

Sequence Viewer

Length: 3414 bp
ATGGCTTCAGCAGGCGGAGGCTCCGGCGGCGGCGGCGGCGGCGGCTGGGGGAAGGCTGAGTCGTCGTCGGGCACCAGCAGTTTCAAGCAGCACAACAGCAGCACTCGGGCGGAGTCGGTGAGCAAAGCGGTGGCGCAGTACACCGTCGACGCTCGGCTGCACGCCGTGTTCGAGCAGTCCGAGTCCGGTAAGTCTTTCGACTACTCGCAGAGCGTGAGAAGCACGAAAGAATCGGTGCCGGAGCAGCAAATCACGGCGTACCTGTCCAAAATCCAGAGGGGCGGCCACATCCAGCCGTTCGGGTGCACGATTGCCGTGGAGGAGTCCACCTTCGGGGTCATTGCCTACAGCGAGAACGCGCGCGACCTGCTCGACCTAATGCCCCAGTCGGTTCCGGTGCTCGAGAGGCGGGAGATCCTCACAATCGGGACCGACCTCAGGACGCTCTTCACGCCTTCCAGCTCGACTTTGCTGGAGAAGGCGTTCGGCGCTCGGGAGATTACTCTTCTCAACCCAGTTTGGATACATTCGAGGGTTTCCGGCAAGCCGTTTTACGCGATTTTGCATAGGATTGATGTTGGGGTTGTCATTGATTTGGAGCCTGCTAGAACAGAGGACCCGGCTCTGTCTATAGCCGGCGCCGTGCAGTCTCAGAAGCTGGCCGTGAGGGCGATTTCGCAGCTGCAGTCACTGCCCGGTGGGGATATTAAGCTTTTGTGTGACACCGTGGTGGAGAGTGTGAGGGAGCTGACTGGGTATGACAGAGTTATGGTGTACAAGTTTCATGAGGATGAGCACGGGGAGGTTTTGGCGGAGAGTAAGAGGCCGGATTTGGAGCCTTATCTTGGCCTGCATTATCCGGCCACGGATATACCTCAGGCGTCGAGGTTTTTGTTCAAGCAGAATAGGGTGAGAATGATTGTGGATTGTCATGCCACGCCGGTTCATGTGATCCAGGATGAAGGGCTTATGCAGCCTTTGTGCTTGGTTGGATCAACGCTGCGTGCGCCCCATGGTTGTCATTCGCAGTATATGGCTAATATGGGCTCCATTGCGTCGTTGGCTTTGGCGGTTATCATTAATGGAAATGATGATGAGGCTATTGGTGGACGGAGCTTGATGAGGCTGTGGGGCTTGGTTGTTTGCCATCACACTTCTGCGAGGTGCATTCCGTTTCCTCTTAGGTATGCTTGTGAGTTTCTAATGCAGGCCTTTGGACTACAGTTGAATATGGAATTGCAGTTGGCTTTACAGATGTCCGAGAAACATGTTTTGAGGACTCAGACTCTGTTGTGTGATATGCTTCTTCGTGACACCCCGACTGGGATTGTTACTCAAAGTCCTAGTATCATGAATCTTGTTAAGTGTGATGGGGCAGCGCTTTACTACCAAGACAAGTACTACCCAACTGGTGTCACCCCCACTGAAGCTCAGATCAGGGACATTGTTGAGTGGTTGTTGGCTTCGCATGGAACTTCGACAGGTTTAAGCACTGACAGTTTGGCTGATGCTGGGTACCCTGGAGCTGCCTCACTTGGTGATGCTGTCTGTGGGATGGCTGCTGCTTATATTACTAAAAGGGACTTTCTGTTCTGGTTCCGATCCCACACCGCAAAAGAGGTGAAATGGGGTGGAGCAAAGCATCATCCTGAGGACAAGGATGATGGGCAGAGGATGCATCCACGTTCTTCCTTTAAGGCGTTTTTGGAAGTGGTTAAAAGCCGTAGTTTGCCATGGGACAATGCAGAAATGGATGCCATACATTCCTTGCAAATTATTTTGCGAGACTCATTTAAGGATGCTGAGACAAACAACTTAAAGGCTGTTACACAGGCCCAGCATCGGGATCTGGAATATCAAGGGATTGACGAGCTCAGCTCAGTGGCAAGAGAAATGGTTAGATTGATAGAGACTGCAACTGCTCCCATATTTGCTGTAGATGTTAATGGGTGTATAAATGGGTGGAATGCAAAGGCTGCAGAGCTGACAGGGCTCTCAGTTGAGGAGGCTACAGGGAAATCCTTGGTACATGATCTGGTTTACAAAGAATCTGAAGAAACTGTTGACAAACTTCTCCTCCACGCTCTGAGAGGAGAAGAAGATAAAAATGTAGAGATAAAACTGAGGACATTTGGCCCAGAGCATGAAAACAAGCCTGTTTTTGTGGTGGTTAATGCTTGCTCTAGCAAGGATTACACTAATAATATCGTGGGAGTTTGCTTTGTTGGTCAGGATGTTACTGGTCAAAAAGTGGTAATGGACAAATTCATAAACATACAAGGTGATTACAAGGCCATTGTTCATAGTCCCAATCCTTTGATCCCGCCCATATTTGCGTCGGATGATAACACATGTTGCTCAGAGTGGAACACTGCCATGGAAAAACTCACTGGGTGGAGCCGTGGAGATATCATTGGAAAAGTGTTGGTTGGGGAGGTCTTTGGCAGTTGTTGTCGACTCAAGGGTCCAGATGCTATAACAAAATTCATGATTGTCTTGCACAACGCCATTGGAGGCTTAGACACAGACAAGTTTCCTTTTTCATTCTTTGACCGGAATGGGAAATATGTACAAGCTCTCTTGACAGCAAATAAGAGGGTGAATATAGACGGCCAGGTTATTGGAGCTTTCTGCTTTCTGCAGATTCCTAGTTCAGAACTGCAGCAAGCTCTTAGAGTTCAGAGACAACAGGAGAAGCAATGTTTCGCTAGGATGAAAGAATTCGCTTACATATGTCAGGAAATAAAAAATCCTTTAAGTGGTATACGCTTTACTAACTCACTTTTGGAGGCTACAGAGTTAACTGAAGATCAAAAGCAGTTCTTGGAGACTAGTGGTGCTTGTGAGAAGCAAATTTTGAAGATTATAAAGGACGTTGATCTGGCTAGCATTGAAGATGGTTCTTTGGAGCTTGAGAAGGAAGAATTCTTACTTGGAAGTGTCATAAATGCTGTTGTTAGCCAAGTAATGTTATTGCTGAGGGAAAGAAATTTGCAATTGATTCGGGATATTCCTGAAGAAGTCAAAACACTAGCTGTTTATGGTGATCAAGTGAGAATTCAGCAGGTCTTAGCTGATTTCTTATTGAATATGGTACGTTATGCACCATCTGAGGAAGGCTGGGTGGAGATTCATGTTCGTCCAATCTTAACTACAATTCCTGATGGACACTCTATGATTCATACAGAATTCAGGTTGGTATGCCCTGGTGAAGGTCTTCCCCCTCAATTAGTTCAAGACATGTTCCATAGTTGTCGATGGATGACTCAGGAGGGCCTAGGACTGAGCATGTGCAGGAAGATTCTAAAGCTCATGAAGGGAGAAGTCCAATATATCAGAGAATCAGAGAGATGTTATTTCTTAGTTATTCTCGAGCTTCCTATGCCTCAGATACATGCAAAGAGTGTTGACTAG

Protein Analysis

1137

Amino Acids

126.0

Weight (kDa)

5.65

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 86 - 202 8.5e-37 PAS fold
GAF PF01590 236 - 413 4.2e-16 GAF domain
PHY PF00360 426 - 600 2.4e-57 Phytochrome region
PAS PF00989 631 - 746 1.1e-24 PAS fold
PAS_4 PF08448 639 - 749 7.6e-06 PAS fold
PAS PF00989 762 - 881 1.1e-18 PAS fold
HisKA PF00512 905 - 967 1.6e-13 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 1015 - 1127 3.4e-13 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2867
AasI GACNNNNNNGTC 1 cut(s) 2463
Acc36I ACCTGC 2 cut(s) 375, 3055
Acc65I GGTACC 1 cut(s) 1515
AccB1I GGYRCC 4 cut(s) 71, 235, 638, 1515
AccI GTMKAC 3 cut(s) 147, 2455, 2764
AccII CGCG 4 cut(s) 359, 361, 363, 557
AclWI GGATC 6 cut(s) 409, 946, 1000, 1596, 1854, 2314
AcoI YGGCCR 4 cut(s) 283, 660, 861, 2611
AcsI RAATTY 8 cut(s) 2264, 2483, 2720, 2853, 2924, 2989, 3057, 3188
AcuI CTGAAG 4 cut(s) 1446, 2073, 2826, 3036
AcyI GRCGYC 2 cut(s) 639, 881
AdeI CACNNNGTG 3 cut(s) 166, 1538, 2394
AfaI GTAC 8 cut(s) 140, 260, 776, 1400, 1517, 2028, 2571, 3096
AfeI AGCGCT 1 cut(s) 1380
AfiI CCNNNNNNNGG 8 cut(s) 333, 438, 845, 1323, 1842, 1843, 2759, 3212
AflIII ACRYGT 3 cut(s) 1267, 2351, 3240
AgsI TTSAA 7 cut(s) 85, 898, 1228, 2860, 2894, 3088, 3236
AhlI ACTAGT 1 cut(s) 2831
AjnI CCWGG 4 cut(s) 954, 1519, 2613, 3205
AjuI GAANNNNNNNTTGG 2 cut(s) 2916, 2948
AleI CACNNNNGTG 1 cut(s) 728
AloI GAACNNNNNNTCC 4 cut(s) 467, 499, 1574, 1606
Alw21I GWGCWC 4 cut(s) 308, 402, 798, 1875
Alw26I GTCTC 6 cut(s) 654, 1779, 1799, 1904, 2677, 2822
Alw44I GTGCAC 1 cut(s) 304
AlwI GGATC 6 cut(s) 409, 946, 1000, 1596, 1854, 2314
AlwNI CAGNNNCTG 3 cut(s) 658, 691, 1288
Ama87I CYCGRG 4 cut(s) 105, 401, 492, 3371
Aor51HI AGCGCT 1 cut(s) 1380
ApaLI GTGCAC 1 cut(s) 304
ApoI RAATTY 8 cut(s) 2264, 2483, 2720, 2853, 2924, 2989, 3057, 3188
AseI ATTAAT 1 cut(s) 1080
Asp700I GAANNNNTTC 3 cut(s) 482, 2720, 3216
Asp718I GGTACC 1 cut(s) 1515
AspA2I CCTAGG 1 cut(s) 3277
AspLEI GCGC 7 cut(s) 136, 361, 363, 491, 641, 1009, 1381
AspS9I GGNCC 6 cut(s) 429, 616, 1834, 2135, 2465, 3274
AsuC2I CCSGG 2 cut(s) 620, 696
AsuHPI GGTGA 9 cut(s) 130, 922, 1408, 1550, 1633, 2294, 2611, 3056, 3221
AsuNHI GCTAGC 1 cut(s) 2885
AvaI CYCGRG 4 cut(s) 105, 401, 492, 3371
AvaII GGWCC 3 cut(s) 429, 616, 2465
AvrII CCTAGG 1 cut(s) 3277
AxyI CCTNAGG 3 cut(s) 437, 876, 1650
BaeGI GKGCMC 2 cut(s) 74, 308
BanI GGYRCC 4 cut(s) 71, 235, 638, 1515
BanII GRGCYC 3 cut(s) 1049, 1875, 1995
BarI GAAGNNNNNNTAC 2 cut(s) 2913, 2945
BbsI GAAGAC 1 cut(s) 3209
Bbv12I GWGCWC 4 cut(s) 308, 402, 798, 1875
BbvCI CCTCAGC 1 cut(s) 2978
BccI CCATC 8 cut(s) 1155, 1364, 1549, 1658, 2891, 3115, 3158, 3252
BcgI CGANNNNNNTGC 2 cut(s) 2984, 3018
BciT130I CCWGG 4 cut(s) 956, 1521, 2615, 3207
BciVI GTATCC 1 cut(s) 516
BclI TGATCA 1 cut(s) 3046
BcnI CCSGG 2 cut(s) 620, 696
BcoDI GTCTC 6 cut(s) 654, 1779, 1799, 1904, 2677, 2822
BcuI ACTAGT 1 cut(s) 2831
BfoI RGCGCY 3 cut(s) 492, 642, 1382
BfuAI ACCTGC 2 cut(s) 375, 3055
BfuI GTATCC 1 cut(s) 516
BglI GCCNNNNNGGC 1 cut(s) 668
BlnI CCTAGG 1 cut(s) 3277
BlpI GCTNAGC 1 cut(s) 1874
BmcAI AGTACT 1 cut(s) 1400
Bme1390I CCNGG 6 cut(s) 620, 696, 956, 1521, 2615, 3207
Bme18I GGWCC 3 cut(s) 429, 616, 2465
BmeT110I CYCGRG 4 cut(s) 105, 401, 492, 3371
BmgT120I GGNCC 6 cut(s) 429, 616, 1834, 2135, 2465, 3274
BmrFI CCNGG 6 cut(s) 620, 696, 956, 1521, 2615, 3207
BmrI ACTGGG 5 cut(s) 379, 509, 762, 1332, 2400
BmsI GCATC 9 cut(s) 1498, 1531, 1651, 1665, 1687, 1744, 1789, 1849, 2461
BmtI GCTAGC 1 cut(s) 2889
BmuI ACTGGG 5 cut(s) 379, 509, 762, 1332, 2400
BpiI GAAGAC 1 cut(s) 3209
BplI GAGNNNNNCTC 2 cut(s) 1862, 1894
BpmI CTGGAG 2 cut(s) 494, 1542
Bpu10I CCTNAGC 1 cut(s) 2978
Bpu1102I GCTNAGC 1 cut(s) 1874
BpuEI CTTGAG 2 cut(s) 2444, 2933
BpuMI CCSGG 2 cut(s) 620, 696
BsaHI GRCGYC 2 cut(s) 639, 881
BsaWI WCCGGW 3 cut(s) 185, 394, 2553
BsaXI ACNNNNNCTCC 4 cut(s) 467, 497, 2061, 2091
Bsc4I CCNNNNNNNGG 8 cut(s) 333, 438, 845, 1323, 1842, 1843, 2759, 3212
Bse118I RCCGGY 2 cut(s) 635, 940
Bse1I ACTGG 7 cut(s) 385, 515, 757, 1327, 1414, 2245, 2395
Bse21I CCTNAGG 3 cut(s) 437, 876, 1650
Bse3DI GCAATG 3 cut(s) 339, 1050, 2705
BseBI CCWGG 4 cut(s) 956, 1521, 2615, 3207
BseLI CCNNNNNNNGG 8 cut(s) 333, 438, 845, 1323, 1842, 1843, 2759, 3212
BseMI GCAATG 3 cut(s) 339, 1050, 2705
BseNI ACTGG 7 cut(s) 385, 515, 757, 1327, 1414, 2245, 2395
BsePI GCGCGC 1 cut(s) 359
BseRI GAGGAG 4 cut(s) 335, 2018, 2066, 2106
BseSI GKGCMC 2 cut(s) 74, 308
BseYI CCCAGC 4 cut(s) 45, 1511, 1836, 3120
BsgI GTGCAG 3 cut(s) 143, 665, 3313
Bsh1236I CGCG 4 cut(s) 359, 361, 363, 557
BshNI GGYRCC 4 cut(s) 71, 235, 638, 1515
BsiHKAI GWGCWC 4 cut(s) 308, 402, 798, 1875
BsiHKCI CYCGRG 4 cut(s) 105, 401, 492, 3371
BslFI GGGAC 5 cut(s) 442, 1454, 1595, 1751, 2292
BslI CCNNNNNNNGG 8 cut(s) 333, 438, 845, 1323, 1842, 1843, 2759, 3212
BsmAI GTCTC 6 cut(s) 654, 1779, 1799, 1904, 2677, 2822
BsmFI GGGAC 5 cut(s) 442, 1454, 1595, 1751, 2292
BsmI GAATGC 2 cut(s) 1167, 1972
BsoBI CYCGRG 4 cut(s) 105, 401, 492, 3371
Bsp1286I GDGCHC 7 cut(s) 74, 308, 402, 798, 1049, 1875, 1995
Bsp1407I TGTACA 2 cut(s) 774, 2569
Bsp1720I GCTNAGC 1 cut(s) 1874
Bsp19I CCATGG 3 cut(s) 1012, 1733, 2376
BspFNI CGCG 4 cut(s) 359, 361, 363, 557
BspHI TCATGA 4 cut(s) 784, 1350, 2487, 3312
BspMAI CTGCAG 4 cut(s) 687, 1981, 2643, 2664
BspMI ACCTGC 2 cut(s) 375, 3055
BspOI GCTAGC 1 cut(s) 2889
BspPI GGATC 6 cut(s) 409, 946, 1000, 1596, 1854, 2314
BspQI GCTCTTC 1 cut(s) 452
BspT107I GGYRCC 4 cut(s) 71, 235, 638, 1515
BsrDI GCAATG 3 cut(s) 339, 1050, 2705
BsrFI RCCGGY 2 cut(s) 635, 940
BsrGI TGTACA 2 cut(s) 774, 2569
BsrI ACTGG 7 cut(s) 385, 515, 757, 1327, 1414, 2245, 2395
BssAI RCCGGY 2 cut(s) 635, 940
BssHII GCGCGC 1 cut(s) 359
BssNAI GTATAC 1 cut(s) 2765
BssNI GRCGYC 2 cut(s) 639, 881
BssT1I CCWWGG 5 cut(s) 1012, 1733, 2022, 2376, 3277
Bst1107I GTATAC 1 cut(s) 2765
Bst2UI CCWGG 4 cut(s) 956, 1521, 2615, 3207
Bst4CI ACNGT 5 cut(s) 145, 727, 1224, 1499, 2062
Bst6I CTCTTC 2 cut(s) 452, 510
BstACI GRCGYC 2 cut(s) 639, 881
BstAPI GCANNNNNTGC 3 cut(s) 691, 1675, 1976
BstAUI TGTACA 2 cut(s) 774, 2569
BstDSI CCRYGG 7 cut(s) 315, 726, 864, 1012, 1733, 2376, 2401
BstENI CCTNNNNNAGG 1 cut(s) 3210
BstFNI CGCG 4 cut(s) 359, 361, 363, 557
BstH2I RGCGCY 3 cut(s) 492, 642, 1382
BstHHI GCGC 7 cut(s) 136, 361, 363, 491, 641, 1009, 1381
BstMAI GTCTC 6 cut(s) 654, 1779, 1799, 1904, 2677, 2822
BstNI CCWGG 4 cut(s) 956, 1521, 2615, 3207
BstNSI RCATGY 5 cut(s) 1271, 2355, 3244, 3292, 3398
BstSCI CCNGG 6 cut(s) 618, 694, 954, 1519, 2613, 3205
BstSLI GKGCMC 2 cut(s) 74, 308
BstUI CGCG 4 cut(s) 359, 361, 363, 557
BstV2I GAAGAC 1 cut(s) 3209
BstX2I RGATCY 2 cut(s) 414, 1846
BstXI CCANNNNNNTGG 1 cut(s) 2621
BstYI RGATCY 2 cut(s) 414, 1846
BstZ17I GTATAC 1 cut(s) 2765
Bsu36I CCTNAGG 3 cut(s) 437, 876, 1650
BsuI GTATCC 1 cut(s) 516
BtgI CCRYGG 7 cut(s) 315, 726, 864, 1012, 1733, 2376, 2401
BtsI GCAGTG 2 cut(s) 689, 2370
BtsIMutI CAGTG 6 cut(s) 689, 1422, 1491, 1887, 2370, 2388
BveI ACCTGC 2 cut(s) 375, 3055
CaiI CAGNNNCTG 3 cut(s) 658, 691, 1288
CciI TCATGA 4 cut(s) 784, 1350, 2487, 3312
CfoI GCGC 7 cut(s) 136, 361, 363, 491, 641, 1009, 1381
Cfr10I RCCGGY 2 cut(s) 635, 940
Cfr13I GGNCC 6 cut(s) 429, 616, 1834, 2135, 2465, 3274
CseI GACGC 5 cut(s) 158, 451, 870, 1044, 2325
Csp6I GTAC 8 cut(s) 139, 259, 775, 1399, 1516, 2027, 2570, 3095
CviQI GTAC 8 cut(s) 139, 259, 775, 1399, 1516, 2027, 2570, 3095
DinI GGCGCC 1 cut(s) 640
DraIII CACNNNGTG 3 cut(s) 166, 1538, 2394
DrdI GACNNNNNNGTC 1 cut(s) 2463
DseDI GACNNNNNNGTC 1 cut(s) 2463
EaeI YGGCCR 4 cut(s) 283, 660, 861, 2611
Eam1104I CTCTTC 2 cut(s) 452, 510
EarI CTCTTC 2 cut(s) 452, 510
EciI GGCGGA 3 cut(s) 30, 125, 827
Ecl136II GAGCTC 1 cut(s) 1873
Eco130I CCWWGG 5 cut(s) 1012, 1733, 2022, 2376, 3277
Eco147I AGGCCT 1 cut(s) 1211
Eco24I GRGCYC 3 cut(s) 1049, 1875, 1995
Eco32I GATATC 1 cut(s) 2410
Eco47I GGWCC 3 cut(s) 429, 616, 2465
Eco47III AGCGCT 1 cut(s) 1380
Eco53kI GAGCTC 1 cut(s) 1873
Eco57I CTGAAG 4 cut(s) 1446, 2073, 2826, 3036
Eco81I CCTNAGG 3 cut(s) 437, 876, 1650
Eco88I CYCGRG 4 cut(s) 105, 401, 492, 3371
EcoICRI GAGCTC 1 cut(s) 1873
EcoNI CCTNNNNNAGG 1 cut(s) 3210
EcoO109I RGGNCCY 2 cut(s) 616, 3274
EcoRI GAATTC 4 cut(s) 2720, 2924, 3057, 3188
EcoRII CCWGG 4 cut(s) 954, 1519, 2613, 3205
EcoRV GATATC 1 cut(s) 2410
EcoT14I CCWWGG 5 cut(s) 1012, 1733, 2022, 2376, 3277
EcoT22I ATGCAT 1 cut(s) 1680
EcoT38I GRGCYC 3 cut(s) 1049, 1875, 1995
EgeI GGCGCC 1 cut(s) 640
EheI GGCGCC 1 cut(s) 640
ErhI CCWWGG 5 cut(s) 1012, 1733, 2022, 2376, 3277
FalI AAGNNNNNCTT 2 cut(s) 2807, 2839
FaqI GGGAC 5 cut(s) 442, 1454, 1595, 1751, 2292
FauI CCCGC 2 cut(s) 402, 2331
FauNDI CATATG 1 cut(s) 2732
FbaI TGATCA 1 cut(s) 3046
FblI GTMKAC 3 cut(s) 147, 2455, 2764
FriOI GRGCYC 3 cut(s) 1049, 1875, 1995
GlaI GCGC 7 cut(s) 135, 360, 362, 490, 640, 1008, 1380
GsaI CCCAGC 4 cut(s) 49, 1515, 1840, 3124
GsuI CTGGAG 2 cut(s) 494, 1542
HaeII RGCGCY 3 cut(s) 492, 642, 1382
HgaI GACGC 5 cut(s) 158, 451, 870, 1044, 2325
HhaI GCGC 7 cut(s) 136, 361, 363, 491, 641, 1009, 1381
Hin1I GRCGYC 2 cut(s) 639, 881
Hin6I GCGC 7 cut(s) 134, 359, 361, 489, 639, 1007, 1379
HinP1I GCGC 7 cut(s) 134, 359, 361, 489, 639, 1007, 1379
HincII GTYRAC 5 cut(s) 148, 2065, 2456, 2802, 3409
HindII GTYRAC 5 cut(s) 148, 2065, 2456, 2802, 3409
HindIII AAGCTT 1 cut(s) 710
HpaI GTTAAC 1 cut(s) 2802
HphI GGTGA 9 cut(s) 130, 922, 1408, 1550, 1633, 2294, 2611, 3056, 3221
Hpy99I CGWCG 7 cut(s) 67, 70, 149, 152, 886, 1060, 2341
HpyAV CCTTC 9 cut(s) 46, 340, 465, 472, 956, 2911, 3110, 3206, 3310
HpyCH4III ACNGT 5 cut(s) 145, 727, 1224, 1499, 2062
HpyCH4IV ACGT 3 cut(s) 1684, 2874, 3097
HpySE526I ACGT 3 cut(s) 1684, 2874, 3097
Hsp92I GRCGYC 2 cut(s) 639, 881
HspAI GCGC 7 cut(s) 134, 359, 361, 489, 639, 1007, 1379
KasI GGCGCC 1 cut(s) 638
KpnI GGTACC 1 cut(s) 1519
KroI GCCGGC 1 cut(s) 635
KroNI GCCGGC 1 cut(s) 637
Ksp22I TGATCA 1 cut(s) 3046
KspAI GTTAAC 1 cut(s) 2802
LguI GCTCTTC 1 cut(s) 452
LweI GCATC 9 cut(s) 1498, 1531, 1651, 1665, 1687, 1744, 1789, 1849, 2461
MaeII ACGT 3 cut(s) 1684, 2874, 3097
MaeIII GTNAC 7 cut(s) 687, 719, 1310, 1330, 1414, 1825, 2236
MauBI CGCGCGCG 1 cut(s) 359
MfeI CAATTG 1 cut(s) 2996
MflI RGATCY 2 cut(s) 414, 1846
MhlI GDGCHC 7 cut(s) 74, 308, 402, 798, 1049, 1875, 1995
Mly113I GGCGCC 1 cut(s) 639
MlyI GAGTC 9 cut(s) 68, 122, 191, 332, 1273, 1279, 1781, 2451, 3259
MmeI TCCRAC 2 cut(s) 970, 2319
Mph1103I ATGCAT 1 cut(s) 1680
MroNI GCCGGC 1 cut(s) 635
MroXI GAANNNNTTC 3 cut(s) 482, 2720, 3216
MslI CAYNNNNRTG 3 cut(s) 728, 789, 2375
MspA1I CMGCKG 1 cut(s) 682
MspR9I CCNGG 6 cut(s) 620, 696, 956, 1521, 2615, 3207
MunI CAATTG 1 cut(s) 2996
Mva1269I GAATGC 2 cut(s) 1167, 1972
MvaI CCWGG 4 cut(s) 956, 1521, 2615, 3207
MvnI CGCG 4 cut(s) 359, 361, 363, 557
NaeI GCCGGC 1 cut(s) 637
NarI GGCGCC 1 cut(s) 639
NciI CCSGG 2 cut(s) 620, 696
NcoI CCATGG 3 cut(s) 1012, 1733, 2376
NdeI CATATG 1 cut(s) 2732
NgoMIV GCCGGC 1 cut(s) 635
NheI GCTAGC 1 cut(s) 2885
NmeAIII GCCGAG 1 cut(s) 133
NmuCI GTSAC 4 cut(s) 687, 719, 1310, 1414
NsiI ATGCAT 1 cut(s) 1680
NspI RCATGY 5 cut(s) 1271, 2355, 3244, 3292, 3398
OliI CACNNNNGTG 1 cut(s) 728
PaeR7I CTCGAG 2 cut(s) 401, 3371
PagI TCATGA 4 cut(s) 784, 1350, 2487, 3312
PauI GCGCGC 1 cut(s) 359
PceI AGGCCT 1 cut(s) 1211
PciI ACATGT 3 cut(s) 1267, 2351, 3240
PciSI GCTCTTC 1 cut(s) 452
PcsI WCGNNNNNNNCGW 2 cut(s) 168, 177
PctI GAATGC 2 cut(s) 1167, 1972
PdiI GCCGGC 1 cut(s) 637
PdmI GAANNNNTTC 3 cut(s) 482, 2720, 3216
PfeI GAWTC 9 cut(s) 230, 1354, 2048, 2644, 3001, 3130, 3178, 3301, 3341
PfoI TCCNGGA 1 cut(s) 954
PleI GAGTC 9 cut(s) 67, 121, 190, 331, 1273, 1279, 1781, 2451, 3259
PluTI GGCGCC 1 cut(s) 642
PpsI GAGTC 9 cut(s) 67, 121, 190, 331, 1273, 1279, 1781, 2451, 3259
PpuMI RGGWCCY 1 cut(s) 616
PscI ACATGT 3 cut(s) 1267, 2351, 3240
PshBI ATTAAT 1 cut(s) 1080
PsiI TTATAA 1 cut(s) 2867
Psp124BI GAGCTC 1 cut(s) 1875
Psp5II RGGWCCY 1 cut(s) 616
Psp6I CCWGG 4 cut(s) 954, 1519, 2613, 3205
PspFI CCCAGC 4 cut(s) 45, 1511, 1836, 3120
PspGI CCWGG 4 cut(s) 954, 1519, 2613, 3205
PspPI GGNCC 6 cut(s) 429, 616, 1834, 2135, 2465, 3274
PspPPI RGGWCCY 1 cut(s) 616
PstI CTGCAG 4 cut(s) 687, 1981, 2643, 2664
PstNI CAGNNNCTG 3 cut(s) 658, 691, 1288
PsuI RGATCY 2 cut(s) 414, 1846
PteI GCGCGC 1 cut(s) 359
PvuII CAGCTG 1 cut(s) 682
RsaI GTAC 8 cut(s) 140, 260, 776, 1400, 1517, 2028, 2571, 3096
RsaNI GTAC 8 cut(s) 139, 259, 775, 1399, 1516, 2027, 2570, 3095
RseI CAYNNNNRTG 3 cut(s) 728, 789, 2375
SacI GAGCTC 1 cut(s) 1875
SalI GTCGAC 2 cut(s) 146, 2454
SapI GCTCTTC 1 cut(s) 452
Sau96I GGNCC 6 cut(s) 429, 616, 1834, 2135, 2465, 3274
ScaI AGTACT 1 cut(s) 1400
SchI GAGTC 9 cut(s) 68, 122, 191, 332, 1273, 1279, 1781, 2451, 3259
ScrFI CCNGG 6 cut(s) 620, 696, 956, 1521, 2615, 3207
SduI GDGCHC 7 cut(s) 74, 308, 402, 798, 1049, 1875, 1995
SfaNI GCATC 9 cut(s) 1498, 1531, 1651, 1665, 1687, 1744, 1789, 1849, 2461
SfoI GGCGCC 1 cut(s) 640
Sfr274I CTCGAG 2 cut(s) 401, 3371
SgrDI CGTCGACG 1 cut(s) 146
SinI GGWCC 3 cut(s) 429, 616, 2465
SlaI CTCGAG 2 cut(s) 401, 3371
SmiMI CAYNNNNRTG 3 cut(s) 728, 789, 2375
SmlI CTYRAG 4 cut(s) 401, 2459, 2912, 3371
SmoI CTYRAG 4 cut(s) 401, 2459, 2912, 3371
SpeI ACTAGT 1 cut(s) 2831
SseBI AGGCCT 1 cut(s) 1211
SspDI GGCGCC 1 cut(s) 638
SstI GAGCTC 1 cut(s) 1875
StuI AGGCCT 1 cut(s) 1211
StyD4I CCNGG 6 cut(s) 618, 694, 954, 1519, 2613, 3205
StyI CCWWGG 5 cut(s) 1012, 1733, 2022, 2376, 3277
TaaI ACNGT 5 cut(s) 145, 727, 1224, 1499, 2062
TaiI ACGT 3 cut(s) 1687, 2877, 3100
TaqII GACCGA 1 cut(s) 446
TatI WGTACW 4 cut(s) 138, 774, 1398, 2569
TauI GCSGC 7 cut(s) 30, 33, 36, 39, 42, 45, 285
TfiI GAWTC 9 cut(s) 230, 1354, 2048, 2644, 3001, 3130, 3178, 3301, 3341
TscAI CASTG 6 cut(s) 696, 1429, 1498, 1887, 2377, 2395
TseFI GTSAC 4 cut(s) 687, 719, 1310, 1414
Tsp45I GTSAC 4 cut(s) 687, 719, 1310, 1414
TspGWI ACGGA 3 cut(s) 881, 1126, 1161
TspRI CASTG 6 cut(s) 696, 1429, 1498, 1887, 2377, 2395
VneI GTGCAC 1 cut(s) 304
VpaK11BI GGWCC 3 cut(s) 429, 616, 2465
VspI ATTAAT 1 cut(s) 1080
XagI CCTNNNNNAGG 1 cut(s) 3210
XapI RAATTY 8 cut(s) 2264, 2483, 2720, 2853, 2924, 2989, 3057, 3188
XceI RCATGY 5 cut(s) 1271, 2355, 3244, 3292, 3398
XcmI CCANNNNNNNNNTGG 1 cut(s) 1057
XhoI CTCGAG 2 cut(s) 401, 3371
XmaJI CCTAGG 1 cut(s) 3277
XmiI GTMKAC 3 cut(s) 147, 2455, 2764
XmnI GAANNNNTTC 3 cut(s) 482, 2720, 3216
ZrmI AGTACT 1 cut(s) 1400
Zsp2I ATGCAT 1 cut(s) 1680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.