Rroxscaffold_5G00367300

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009857
Physical Location & Seq
Forward (+)
49144275 .. 49149962
5688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00367300.1

Sequence Viewer

Length: 3399 bp
ATGGCATCAGCAGGCGGAGGCGGCGGCGGCTGGGGGAAGGCTCAGTCGTCTTCGGGCACCAGCAATTTCAAGCAGCACAGCAGCAGCACTCCGGCGGAGTCGGTGAGCAAAGCGGTGGCGCAGTACACGGTCGACGCTCGGCTGCACGCCGTGTTCGAGCAGGCGGAGTCGGGTAAGTCTTTCGACTACTCGCAGAGCATGAGAAGCACCAAAGAATCGGTGCCGGAGCAGCAAATCACGGCGTACCTGTCCAGAATCCAGAGGGGCGGCCATATCCAGCCCTTCGGGTGCACGATTGCGGTGGAAGAGTCGACCTTCGGGGTCATTGCCTACAGCGAGAACGCCCGCGACCTGCTCGACCTGATGCCCCAGTCGGTTCCGGTGCTCGAGAGGCGGGAGATCCTCACAATCGGGACCGACGTCAGGACGCTCTTCATGCCTTCCAGCTCAACTTTGCTGGAGAGGGCGTTTGGCGCTCGGGAGATTACTCTTCTCAACCCAATTTGGATACATTCGAGGGTTTCCGGCAAGCCGTTTTACGCGATTTTGCATAGGATTGATGTTGGGGTTGTGATTGATTTGGAGCCTGCTAGAACAGAGGACCCGGCTCTGTCTATAGCCGGGGCCGTGCAGTCTCAGAAGCTGGCCGTGAGGGCGATTTCGCAGCTGCAGTCACTACCCGGTGGGGATGTTAAGCTTTTGTGTGACACGGTGGTGGAGAGTGTGAGGGAGCTGACTGGGTATGATAGAGTTATGGTGTACAAGTTTCATGAGGATGAGCACGGGGAGGTTTTGGCGGAGAGTAAGAGGCCGGATTTGGAGCCTTATCTTGGGCTGCATTATCCGGCCACGGATATACCTCAGGCGTCGAGGTTTTTGTTCAAGCAAAATAGGGTGAGAATGATTGTGGATTGTCATGCCACGCCGGTTCAAGTGATCCAGGATGAAGGCCTTATGCAGCCTTTGTGCTTGGTTGGATCAACGCTGCGTGCGCCGCATGGTTGTCATTCGCAGTATATGGCTAATATGGGCTCCATTGCCTCCTTGGCTTTGGCGGTTATCATTAATGGAAATGATGATGAGGCTATTGGTGGACGGAGCTCGATGAGGCTGTGGGGCTTGGTTGTTTGCCATCACACTTCTGCCAGGTGCATTCCGTTCCCTCTTCGGTATGCTTGTGAGTTTCTAATGCAGGCCTTTGGACTACAGTTGAATATGGAATTGCAGTTGGCTTCACAGATGTCGGAGAAACATGTTTTGAGGACTCAGACTCTGTTGTGTGATATGCTTCTTCGTGACACCCCGACTGGGATTATTACTCAGAGTCCTAGTATCATGAATCTTGTCAAGTGTGATGGGGCAGCGCTTTACTACCAAGACAAGTACTACCCAATTGGTGTCAGCCCCACTGAAGCTCAGATCAGGGACATTGTTGAGTGGTTGTTGGCTTCACATGGAACTTCGACAGGTTTAAGCACTGACAGTTTGGCTGATGCTGGCTACCCTGGAGCTGCCTCACTTGGTGATGCTGTCTGTGGGATGGCTGCTGCTTATATTACTAAAAGGGACTTTCTGTTCTGGTTCCGATCCCACACCGCAAAAGAGGTGAAATGGGGTGGAGCAAAGCATCATCCTGAGGACAAGGATGATGGGCAGAGGATGCATCCACGTTCTTCCTTTAAGGCGTTTTTGGAAGTGGTTAAAAGCCGTAGTTTGCCATGGGACAATGCAGAAATGGATGCCATACATTCCTTGCAAATTATTTTGCGAGACTCATTTAAGGATGCTGAGACAAACAACTTAAAGGCTGTTACACAGGCCCAGCATGGGGATCTGGAATGTCAAGGGATTGACGAGCTCAGCTCAGTGGCAAGAGAAATGGTTAGATTGATAGAGACTGCAACTGCTCCCATTTTTGCTGTAGATGTTAATGGGTGTATAAATGGGTGGAATGCAAAGGTTGCAGAGCTGACAGGGCTCTCAGTTGAGGAGGCTACAGGGAAATCCTTGGTACATGATCTGGTTTACAAAGAATCTGAAGAAACTGTTGACAAACTTCTCCTCCACGCTCTGAGAGGAGAAGAAGATAAAAATGTAGAGATAAAACTGAGGACATTTGGCCCAGAGCATGAAAACAAGCCTGTTTTTGTGGTGGTTAATGCTTGCTCTAGCAAGGATTACATTAATAATATCGTGGGAGTTTGCTTTGTTGGTCAGGATGTTACTGGTCAAAAAGTGGTAATGGACAAATTCATAAACATACAAGGTGATTACAAGGCCATTGTTCATAGTCCAAATCCTTTGATCCCGCCCATATTTGCGTCAGATGATAACACATGTTGCTCAGAGTGGAACACTGCCATGGAAAAACTCACTGGGTGGAGCCGTGCAGATATCATTGGAAAAGTGTTGGTTGGGGAGGTCTTTGGCAGTTGTTGTCGACTCAAGGGTCCAGATGCTATAACAAAATTCATGATTGTCTTGCACAACGCCATTGGAGGCTTAGACACGGACAAGTTTCCTTTTTCGTTCTTTGACCAGAATGGGAAATTTGTACAAGCTCTCTTGACAGCAAATAAGAGGGTGAATATAGACGGCCAGGTTATTGGAGCTTTCTGCTTTCTGCAGATTCCTAGTCCAGAACTGCAGCAAGCTCTTAGAGTTCAGAGACAACAGGAGAAGCAATGTTTCACTAGGATGAAAGAATTCGCTTACATATGTCAGGAAATAAAAAATCCTCTAAGTGGTATACGCTTTACTAACTCACTTTTGGAGGCTACAGAGTTGACTGAAGATCAAAAGCAGTTCTTGGAGACTAGTGGTGCTTGTGAGAAGCAAATTTTGAAGATTATAAAGGACGTTGATCTGGCTAGCATTGAAGATGGTTCTTTGGAGCTTGAGAAGGAAGAATTCTTACTTGGAAGTGTCATAAATGCTGTTGTTAGTCAAGTAATGTTATTGCTGAGGGAAAGAAATTTGCAATTGATTCGGGATATTCCTGAAGAAGTCAAAACACTAGCTGTTTATGGTGATCAAGTGAGAATTCAGCAGGTCTTAGCTGATTTCTTATTGAATATGGTACGTTATGCACCATCTGAGGAAGGCTGGGTGGAGATTCATGTTCGTCCAATCTTAACTACAATTCCTGATGGACACTCTATGGTTCATACAGAATTCAGGTTGGTATGCCCTGGTGAAGGTCTTCCCCCTCAATTAGTTCAAGACATGTTCCATAGTAGTCGATGGCTGACTCAGGAGGGCCTAGGACTGAGCATGTGCAGGAAGATTCTAAAGCTTATGAAGGGAGAAGTCCAATATATCAGAGAATCAGAGAGATGTTATTTCTTAGTTATTCTTGAGCTTCCTATGCCTCGGATACATGCAAAGAGCGTTGACTAG

Protein Analysis

1132

Amino Acids

125.53

Weight (kDa)

5.59

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 81 - 197 3.6e-36 PAS fold
GAF PF01590 233 - 408 4.7e-16 GAF domain
PHY PF00360 421 - 595 7.9e-57 Phytochrome region
PAS PF00989 626 - 741 3.5e-23 PAS fold
PAS PF00989 757 - 876 1.1e-18 PAS fold
HisKA PF00512 901 - 962 1.9e-13 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 1010 - 1122 5.1e-13 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2852
AasI GACNNNNNNGTC 1 cut(s) 2448
AatII GACGTC 1 cut(s) 423
Acc36I ACCTGC 2 cut(s) 360, 3040
AccB1I GGYRCC 2 cut(s) 56, 220
AccI GTMKAC 4 cut(s) 132, 311, 2440, 2749
AccII CGCG 2 cut(s) 348, 542
AclWI GGATC 6 cut(s) 394, 931, 985, 1581, 1839, 2299
AcoI YGGCCR 4 cut(s) 268, 645, 846, 2596
AcsI RAATTY 9 cut(s) 2249, 2468, 2549, 2705, 2838, 2909, 2974, 3042, 3173
AcuI CTGAAG 4 cut(s) 1431, 2058, 2811, 3021
AcyI GRCGYC 2 cut(s) 420, 866
AdeI CACNNNGTG 3 cut(s) 151, 1523, 2379
AfaI GTAC 7 cut(s) 125, 245, 761, 1385, 2013, 2556, 3081
AfeI AGCGCT 1 cut(s) 1365
AfiI CCNNNNNNNGG 7 cut(s) 423, 830, 1308, 1827, 1828, 2744, 3197
AflIII ACRYGT 3 cut(s) 1252, 2336, 3225
AgsI TTSAA 8 cut(s) 70, 883, 932, 1213, 2845, 2879, 3073, 3221
AhlI ACTAGT 1 cut(s) 2816
AjnI CCWGG 5 cut(s) 939, 1145, 1504, 2598, 3190
AjuI GAANNNNNNNTTGG 2 cut(s) 2901, 2933
AleI CACNNNNGTG 1 cut(s) 713
AloI GAACNNNNNNTCC 2 cut(s) 1559, 1591
Alw21I GWGCWC 5 cut(s) 293, 387, 783, 1103, 1860
Alw26I GTCTC 6 cut(s) 639, 1764, 1784, 1889, 2662, 2807
Alw44I GTGCAC 1 cut(s) 289
AlwI GGATC 6 cut(s) 394, 931, 985, 1581, 1839, 2299
AlwNI CAGNNNCTG 2 cut(s) 643, 1273
Ama87I CYCGRG 2 cut(s) 386, 477
Aor51HI AGCGCT 1 cut(s) 1365
ApaLI GTGCAC 1 cut(s) 289
ApoI RAATTY 9 cut(s) 2249, 2468, 2549, 2705, 2838, 2909, 2974, 3042, 3173
AseI ATTAAT 2 cut(s) 1065, 2184
Asp700I GAANNNNTTC 2 cut(s) 2705, 3201
AspA2I CCTAGG 1 cut(s) 3262
AspLEI GCGC 4 cut(s) 121, 476, 994, 1366
AspS9I GGNCC 7 cut(s) 414, 601, 624, 1819, 2120, 2450, 3259
AsuC2I CCSGG 3 cut(s) 605, 622, 681
AsuHPI GGTGA 8 cut(s) 115, 907, 1535, 1618, 2279, 2596, 3041, 3206
AsuNHI GCTAGC 1 cut(s) 2870
AvaI CYCGRG 2 cut(s) 386, 477
AvaII GGWCC 3 cut(s) 414, 601, 2450
AvrII CCTAGG 1 cut(s) 3262
AxyI CCTNAGG 2 cut(s) 861, 1635
BaeGI GKGCMC 2 cut(s) 59, 293
BanI GGYRCC 2 cut(s) 56, 220
BanII GRGCYC 4 cut(s) 1034, 1103, 1860, 1980
BarI GAAGNNNNNNTAC 2 cut(s) 2898, 2930
BbsI GAAGAC 2 cut(s) 42, 3194
Bbv12I GWGCWC 5 cut(s) 293, 387, 783, 1103, 1860
BbvCI CCTCAGC 1 cut(s) 2963
BccI CCATC 8 cut(s) 1140, 1349, 1534, 1643, 2876, 3100, 3143, 3237
BceAI ACGGC 8 cut(s) 134, 255, 517, 611, 632, 1692, 2370, 2611
BcgI CGANNNNNNTGC 2 cut(s) 2969, 3003
BciT130I CCWGG 5 cut(s) 941, 1147, 1506, 2600, 3192
BciVI GTATCC 2 cut(s) 501, 3369
BclI TGATCA 1 cut(s) 3031
BcnI CCSGG 3 cut(s) 605, 622, 681
BcoDI GTCTC 6 cut(s) 639, 1764, 1784, 1889, 2662, 2807
BcuI ACTAGT 1 cut(s) 2816
BfmI CTRYAG 9 cut(s) 331, 615, 668, 1205, 1920, 1995, 2624, 2645, 2778
BfoI RGCGCY 2 cut(s) 477, 1367
BfuAI ACCTGC 2 cut(s) 360, 3040
BfuI GTATCC 2 cut(s) 501, 3369
BglI GCCNNNNNGGC 2 cut(s) 653, 1046
BlnI CCTAGG 1 cut(s) 3262
BlpI GCTNAGC 1 cut(s) 1859
BmcAI AGTACT 1 cut(s) 1385
Bme1390I CCNGG 8 cut(s) 605, 622, 681, 941, 1147, 1506, 2600, 3192
Bme18I GGWCC 3 cut(s) 414, 601, 2450
BmeT110I CYCGRG 2 cut(s) 386, 477
BmgT120I GGNCC 7 cut(s) 414, 601, 624, 1819, 2120, 2450, 3259
BmrFI CCNGG 8 cut(s) 605, 622, 681, 941, 1147, 1506, 2600, 3192
BmrI ACTGGG 4 cut(s) 364, 747, 1317, 2385
BmtI GCTAGC 1 cut(s) 2874
BmuI ACTGGG 4 cut(s) 364, 747, 1317, 2385
BoxI GACNNNNGTC 1 cut(s) 419
BpiI GAAGAC 2 cut(s) 42, 3194
BplI GAGNNNNNCTC 2 cut(s) 1847, 1879
BpmI CTGGAG 2 cut(s) 479, 1527
Bpu10I CCTNAGC 1 cut(s) 2963
Bpu1102I GCTNAGC 1 cut(s) 1859
BpuEI CTTGAG 3 cut(s) 2429, 2918, 3377
BpuMI CCSGG 3 cut(s) 605, 622, 681
BsaHI GRCGYC 2 cut(s) 420, 866
BsaWI WCCGGW 1 cut(s) 379
BsaXI ACNNNNNCTCC 4 cut(s) 452, 482, 2046, 2076
Bsc4I CCNNNNNNNGG 7 cut(s) 423, 830, 1308, 1827, 1828, 2744, 3197
Bse118I RCCGGY 1 cut(s) 925
Bse1I ACTGG 5 cut(s) 370, 742, 1312, 2230, 2380
Bse21I CCTNAGG 2 cut(s) 861, 1635
Bse3DI GCAATG 3 cut(s) 324, 1035, 2690
BseBI CCWGG 5 cut(s) 941, 1147, 1506, 2600, 3192
BseLI CCNNNNNNNGG 7 cut(s) 423, 830, 1308, 1827, 1828, 2744, 3197
BseMI GCAATG 3 cut(s) 324, 1035, 2690
BseNI ACTGG 5 cut(s) 370, 742, 1312, 2230, 2380
BseRI GAGGAG 3 cut(s) 2003, 2051, 2091
BseSI GKGCMC 2 cut(s) 59, 293
BseYI CCCAGC 3 cut(s) 30, 1821, 3105
BsgI GTGCAG 4 cut(s) 128, 650, 2409, 3298
Bsh1236I CGCG 2 cut(s) 348, 542
Bsh1285I CGRYCG 1 cut(s) 132
BshNI GGYRCC 2 cut(s) 56, 220
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 5 cut(s) 293, 387, 783, 1103, 1860
BsiHKCI CYCGRG 2 cut(s) 386, 477
BslFI GGGAC 4 cut(s) 427, 1439, 1580, 1736
BslI CCNNNNNNNGG 7 cut(s) 423, 830, 1308, 1827, 1828, 2744, 3197
BsmAI GTCTC 6 cut(s) 639, 1764, 1784, 1889, 2662, 2807
BsmFI GGGAC 4 cut(s) 427, 1439, 1580, 1736
BsmI GAATGC 2 cut(s) 1152, 1957
BsoBI CYCGRG 2 cut(s) 386, 477
Bsp1286I GDGCHC 8 cut(s) 59, 293, 387, 783, 1034, 1103, 1860, 1980
Bsp1407I TGTACA 2 cut(s) 759, 2554
Bsp1720I GCTNAGC 1 cut(s) 1859
Bsp19I CCATGG 2 cut(s) 1718, 2361
BspFNI CGCG 2 cut(s) 348, 542
BspHI TCATGA 3 cut(s) 769, 1335, 2472
BspMAI CTGCAG 3 cut(s) 672, 2628, 2649
BspMI ACCTGC 2 cut(s) 360, 3040
BspOI GCTAGC 1 cut(s) 2874
BspPI GGATC 6 cut(s) 394, 931, 985, 1581, 1839, 2299
BspQI GCTCTTC 1 cut(s) 437
BspT107I GGYRCC 2 cut(s) 56, 220
BsrDI GCAATG 3 cut(s) 324, 1035, 2690
BsrFI RCCGGY 1 cut(s) 925
BsrGI TGTACA 2 cut(s) 759, 2554
BsrI ACTGG 5 cut(s) 370, 742, 1312, 2230, 2380
BssAI RCCGGY 1 cut(s) 925
BssNAI GTATAC 1 cut(s) 2750
BssNI GRCGYC 2 cut(s) 420, 866
BssT1I CCWWGG 5 cut(s) 1044, 1718, 2007, 2361, 3262
Bst1107I GTATAC 1 cut(s) 2750
Bst2UI CCWGG 5 cut(s) 941, 1147, 1506, 2600, 3192
Bst4CI ACNGT 5 cut(s) 130, 712, 1209, 1484, 2047
Bst6I CTCTTC 4 cut(s) 300, 437, 495, 1170
BstACI GRCGYC 2 cut(s) 420, 866
BstAPI GCANNNNNTGC 2 cut(s) 1660, 1961
BstAUI TGTACA 2 cut(s) 759, 2554
BstDSI CCRYGG 3 cut(s) 849, 1718, 2361
BstENI CCTNNNNNAGG 1 cut(s) 3195
BstFNI CGCG 2 cut(s) 348, 542
BstH2I RGCGCY 2 cut(s) 477, 1367
BstHHI GCGC 4 cut(s) 121, 476, 994, 1366
BstMAI GTCTC 6 cut(s) 639, 1764, 1784, 1889, 2662, 2807
BstMCI CGRYCG 1 cut(s) 132
BstNI CCWGG 5 cut(s) 941, 1147, 1506, 2600, 3192
BstNSI RCATGY 5 cut(s) 1256, 2340, 3229, 3277, 3383
BstPAI GACNNNNGTC 1 cut(s) 419
BstSCI CCNGG 8 cut(s) 603, 620, 679, 939, 1145, 1504, 2598, 3190
BstSFI CTRYAG 9 cut(s) 331, 615, 668, 1205, 1920, 1995, 2624, 2645, 2778
BstSLI GKGCMC 2 cut(s) 59, 293
BstUI CGCG 2 cut(s) 348, 542
BstV2I GAAGAC 2 cut(s) 42, 3194
BstX2I RGATCY 2 cut(s) 399, 1831
BstXI CCANNNNNNTGG 1 cut(s) 2606
BstYI RGATCY 2 cut(s) 399, 1831
BstZ17I GTATAC 1 cut(s) 2750
Bsu36I CCTNAGG 2 cut(s) 861, 1635
BsuI GTATCC 2 cut(s) 501, 3369
BtgI CCRYGG 3 cut(s) 849, 1718, 2361
BtsI GCAGTG 1 cut(s) 2355
BtsIMutI CAGTG 5 cut(s) 1407, 1476, 1872, 2355, 2373
BveI ACCTGC 2 cut(s) 360, 3040
CaiI CAGNNNCTG 2 cut(s) 643, 1273
CciI TCATGA 3 cut(s) 769, 1335, 2472
CfoI GCGC 4 cut(s) 121, 476, 994, 1366
Cfr10I RCCGGY 1 cut(s) 925
Cfr13I GGNCC 7 cut(s) 414, 601, 624, 1819, 2120, 2450, 3259
CseI GACGC 4 cut(s) 143, 436, 855, 2310
Csp6I GTAC 7 cut(s) 124, 244, 760, 1384, 2012, 2555, 3080
CviQI GTAC 7 cut(s) 124, 244, 760, 1384, 2012, 2555, 3080
DraIII CACNNNGTG 3 cut(s) 151, 1523, 2379
DrdI GACNNNNNNGTC 1 cut(s) 2448
DseDI GACNNNNNNGTC 1 cut(s) 2448
EaeI YGGCCR 4 cut(s) 268, 645, 846, 2596
Eam1104I CTCTTC 4 cut(s) 300, 437, 495, 1170
EarI CTCTTC 4 cut(s) 300, 437, 495, 1170
EciI GGCGGA 4 cut(s) 30, 110, 179, 812
Ecl136II GAGCTC 2 cut(s) 1101, 1858
Eco130I CCWWGG 5 cut(s) 1044, 1718, 2007, 2361, 3262
Eco147I AGGCCT 2 cut(s) 951, 1196
Eco24I GRGCYC 4 cut(s) 1034, 1103, 1860, 1980
Eco32I GATATC 1 cut(s) 2395
Eco47I GGWCC 3 cut(s) 414, 601, 2450
Eco47III AGCGCT 1 cut(s) 1365
Eco53kI GAGCTC 2 cut(s) 1101, 1858
Eco57I CTGAAG 4 cut(s) 1431, 2058, 2811, 3021
Eco81I CCTNAGG 2 cut(s) 861, 1635
Eco88I CYCGRG 2 cut(s) 386, 477
EcoICRI GAGCTC 2 cut(s) 1101, 1858
EcoNI CCTNNNNNAGG 1 cut(s) 3195
EcoO109I RGGNCCY 2 cut(s) 601, 3259
EcoRI GAATTC 4 cut(s) 2705, 2909, 3042, 3173
EcoRII CCWGG 5 cut(s) 939, 1145, 1504, 2598, 3190
EcoRV GATATC 1 cut(s) 2395
EcoT14I CCWWGG 5 cut(s) 1044, 1718, 2007, 2361, 3262
EcoT22I ATGCAT 1 cut(s) 1665
EcoT38I GRGCYC 4 cut(s) 1034, 1103, 1860, 1980
ErhI CCWWGG 5 cut(s) 1044, 1718, 2007, 2361, 3262
FalI AAGNNNNNCTT 2 cut(s) 2792, 2824
FaqI GGGAC 4 cut(s) 427, 1439, 1580, 1736
FauI CCCGC 3 cut(s) 353, 387, 2316
FauNDI CATATG 1 cut(s) 2717
FbaI TGATCA 1 cut(s) 3031
FblI GTMKAC 4 cut(s) 132, 311, 2440, 2749
FriOI GRGCYC 4 cut(s) 1034, 1103, 1860, 1980
GlaI GCGC 4 cut(s) 120, 475, 993, 1365
GsaI CCCAGC 3 cut(s) 34, 1825, 3109
GsuI CTGGAG 2 cut(s) 479, 1527
HaeII RGCGCY 2 cut(s) 477, 1367
HgaI GACGC 4 cut(s) 143, 436, 855, 2310
HhaI GCGC 4 cut(s) 121, 476, 994, 1366
Hin1I GRCGYC 2 cut(s) 420, 866
Hin6I GCGC 4 cut(s) 119, 474, 992, 1364
HinP1I GCGC 4 cut(s) 119, 474, 992, 1364
HincII GTYRAC 6 cut(s) 133, 312, 2050, 2441, 2787, 3394
HindII GTYRAC 6 cut(s) 133, 312, 2050, 2441, 2787, 3394
HindIII AAGCTT 2 cut(s) 695, 3293
HphI GGTGA 8 cut(s) 115, 907, 1535, 1618, 2279, 2596, 3041, 3206
Hpy99I CGWCG 3 cut(s) 137, 422, 871
HpyAV CCTTC 9 cut(s) 31, 292, 325, 450, 941, 2896, 3095, 3191, 3295
HpyCH4III ACNGT 5 cut(s) 130, 712, 1209, 1484, 2047
HpyCH4IV ACGT 4 cut(s) 420, 1669, 2859, 3082
HpySE526I ACGT 4 cut(s) 420, 1669, 2859, 3082
Hsp92I GRCGYC 2 cut(s) 420, 866
HspAI GCGC 4 cut(s) 119, 474, 992, 1364
Ksp22I TGATCA 1 cut(s) 3031
LguI GCTCTTC 1 cut(s) 437
MaeII ACGT 4 cut(s) 420, 1669, 2859, 3082
MaeIII GTNAC 5 cut(s) 672, 704, 1295, 1810, 2221
MfeI CAATTG 2 cut(s) 1392, 2981
MflI RGATCY 2 cut(s) 399, 1831
MhlI GDGCHC 8 cut(s) 59, 293, 387, 783, 1034, 1103, 1860, 1980
MlyI GAGTC 9 cut(s) 107, 176, 317, 1258, 1264, 1333, 1766, 2436, 3244
MmeI TCCRAC 2 cut(s) 955, 1224
Mph1103I ATGCAT 1 cut(s) 1665
MroXI GAANNNNTTC 2 cut(s) 2705, 3201
MslI CAYNNNNRTG 4 cut(s) 713, 774, 2360, 2696
MspA1I CMGCKG 1 cut(s) 667
MspR9I CCNGG 8 cut(s) 605, 622, 681, 941, 1147, 1506, 2600, 3192
MunI CAATTG 2 cut(s) 1392, 2981
Mva1269I GAATGC 2 cut(s) 1152, 1957
MvaI CCWGG 5 cut(s) 941, 1147, 1506, 2600, 3192
MvnI CGCG 2 cut(s) 348, 542
NciI CCSGG 3 cut(s) 605, 622, 681
NcoI CCATGG 2 cut(s) 1718, 2361
NdeI CATATG 1 cut(s) 2717
NheI GCTAGC 1 cut(s) 2870
NmeAIII GCCGAG 1 cut(s) 118
NmuCI GTSAC 3 cut(s) 672, 704, 1295
NsiI ATGCAT 1 cut(s) 1665
NspI RCATGY 5 cut(s) 1256, 2340, 3229, 3277, 3383
OliI CACNNNNGTG 1 cut(s) 713
PaeR7I CTCGAG 1 cut(s) 386
PagI TCATGA 3 cut(s) 769, 1335, 2472
PceI AGGCCT 2 cut(s) 951, 1196
PciI ACATGT 3 cut(s) 1252, 2336, 3225
PciSI GCTCTTC 1 cut(s) 437
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 417
PctI GAATGC 2 cut(s) 1152, 1957
PdmI GAANNNNTTC 2 cut(s) 2705, 3201
PfeI GAWTC 9 cut(s) 215, 255, 1339, 2033, 2629, 2986, 3115, 3286, 3326
PfoI TCCNGGA 1 cut(s) 939
PleI GAGTC 9 cut(s) 106, 175, 316, 1258, 1264, 1332, 1766, 2436, 3244
PpsI GAGTC 9 cut(s) 106, 175, 316, 1258, 1264, 1332, 1766, 2436, 3244
PpuMI RGGWCCY 1 cut(s) 601
PscI ACATGT 3 cut(s) 1252, 2336, 3225
PshAI GACNNNNGTC 1 cut(s) 419
PshBI ATTAAT 2 cut(s) 1065, 2184
PsiI TTATAA 1 cut(s) 2852
Psp124BI GAGCTC 2 cut(s) 1103, 1860
Psp5II RGGWCCY 1 cut(s) 601
Psp6I CCWGG 5 cut(s) 939, 1145, 1504, 2598, 3190
PspFI CCCAGC 3 cut(s) 30, 1821, 3105
PspGI CCWGG 5 cut(s) 939, 1145, 1504, 2598, 3190
PspPI GGNCC 7 cut(s) 414, 601, 624, 1819, 2120, 2450, 3259
PspPPI RGGWCCY 1 cut(s) 601
PstI CTGCAG 3 cut(s) 672, 2628, 2649
PstNI CAGNNNCTG 2 cut(s) 643, 1273
PsuI RGATCY 2 cut(s) 399, 1831
PvuII CAGCTG 1 cut(s) 667
RsaI GTAC 7 cut(s) 125, 245, 761, 1385, 2013, 2556, 3081
RsaNI GTAC 7 cut(s) 124, 244, 760, 1384, 2012, 2555, 3080
RseI CAYNNNNRTG 4 cut(s) 713, 774, 2360, 2696
SacI GAGCTC 2 cut(s) 1103, 1860
SalI GTCGAC 3 cut(s) 131, 310, 2439
SapI GCTCTTC 1 cut(s) 437
Sau96I GGNCC 7 cut(s) 414, 601, 624, 1819, 2120, 2450, 3259
ScaI AGTACT 1 cut(s) 1385
SchI GAGTC 9 cut(s) 107, 176, 317, 1258, 1264, 1333, 1766, 2436, 3244
ScrFI CCNGG 8 cut(s) 605, 622, 681, 941, 1147, 1506, 2600, 3192
SduI GDGCHC 8 cut(s) 59, 293, 387, 783, 1034, 1103, 1860, 1980
SfcI CTRYAG 9 cut(s) 331, 615, 668, 1205, 1920, 1995, 2624, 2645, 2778
Sfr274I CTCGAG 1 cut(s) 386
SinI GGWCC 3 cut(s) 414, 601, 2450
SlaI CTCGAG 1 cut(s) 386
SmiMI CAYNNNNRTG 4 cut(s) 713, 774, 2360, 2696
SmlI CTYRAG 4 cut(s) 386, 2444, 2897, 3356
SmoI CTYRAG 4 cut(s) 386, 2444, 2897, 3356
SpeI ACTAGT 1 cut(s) 2816
SseBI AGGCCT 2 cut(s) 951, 1196
SstI GAGCTC 2 cut(s) 1103, 1860
StuI AGGCCT 2 cut(s) 951, 1196
StyD4I CCNGG 8 cut(s) 603, 620, 679, 939, 1145, 1504, 2598, 3190
StyI CCWWGG 5 cut(s) 1044, 1718, 2007, 2361, 3262
TaaI ACNGT 5 cut(s) 130, 712, 1209, 1484, 2047
TaiI ACGT 4 cut(s) 423, 1672, 2862, 3085
TaqII GACCGA 1 cut(s) 431
TatI WGTACW 4 cut(s) 123, 759, 1383, 2554
TauI GCSGC 5 cut(s) 24, 27, 30, 270, 997
TfiI GAWTC 9 cut(s) 215, 255, 1339, 2033, 2629, 2986, 3115, 3286, 3326
TscAI CASTG 5 cut(s) 1414, 1483, 1872, 2362, 2380
TseFI GTSAC 3 cut(s) 672, 704, 1295
Tsp45I GTSAC 3 cut(s) 672, 704, 1295
TspGWI ACGGA 4 cut(s) 866, 1111, 1146, 2525
TspRI CASTG 5 cut(s) 1414, 1483, 1872, 2362, 2380
VneI GTGCAC 1 cut(s) 289
VpaK11BI GGWCC 3 cut(s) 414, 601, 2450
VspI ATTAAT 2 cut(s) 1065, 2184
XagI CCTNNNNNAGG 1 cut(s) 3195
XapI RAATTY 9 cut(s) 2249, 2468, 2549, 2705, 2838, 2909, 2974, 3042, 3173
XceI RCATGY 5 cut(s) 1256, 2340, 3229, 3277, 3383
XcmI CCANNNNNNNNNTGG 1 cut(s) 1042
XhoI CTCGAG 1 cut(s) 386
XmaJI CCTAGG 1 cut(s) 3262
XmiI GTMKAC 4 cut(s) 132, 311, 2440, 2749
XmnI GAANNNNTTC 2 cut(s) 2705, 3201
ZraI GACGTC 1 cut(s) 421
ZrmI AGTACT 1 cut(s) 1385
Zsp2I ATGCAT 1 cut(s) 1665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.