Rh4DG261000

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0009857
Physical Location & Seq
Forward (+)
47861634 .. 47867228
5595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG261000.1

Sequence Viewer

Length: 2892 bp
ATGGCTTCAGCAGGCGGAGGCTCCGGCGGCGGCTGGGGGAAGGCTCAGTCGTCTTCGGGCACCAGCAATTTCAAGCAGCACAACAGCAGCACTCCGGCGGAGTCGGTGAGCAAAGCGGTGGCGCAGTACACGGTCGACGCTCGGCTGCACGCCGTGTTCGAGCAGTCCGAGTCCGGCAAGTCTTTCGACTACTCGCAGAGCATGAGAAGCACGAAAGAATCCGTGCCGGAGCAGCAAATCACGGCGTACCTGTCCAAAATCCAGAGGGGCGGCCATATCCAGCCCTTCGGGTGCACGATTGCCGTGGAGGAGTCCACCTTCGGGGTCATTGCCTACAGCGAGAACGCGCGCGACCTGCTCGACCTAATGCCCCAGTCGGTTCCGGTGCTCGAGAGGCGGGAGATCCTCACAATCGGGACCGACCTCAGGACGCTCTTCACGCCTTCCAGCTCGACGTTGCTGGAGAAGGCGTTAGGCGCTCGGGAGATTACTCTTCTCAACCCAATTTGGATACATTCGAGGGTTTCCGGCAAGCCGTTTTACGCGATTTTGCATAGGATTGATGTTGGGGTTGTCATTGATTTGGAGCCTGCTAGAACAGAGGACCCGGCTCTGTCTATAGCCGGGGCCGTGCAGTCTCAGAAGCTGGCCGTGAGGGCGATTTCGCAGCTGCAGTCACTGCCCGGTGGGGATATTAAGCTTTTGTGTGACACGGTGGTGGAGAGTGTGAGGGAGCTGACTGGGTATGATAGAGTTATGGTGTACAAGTTTCATGAGGATGAGCACGGGGAGGTTTTGGCGGAGAGTAAGAGGCCGGATTTGGAGCCTTATCTTGGGCTGCATTATCCGGCCACGGATATACCTCAGGCGTCGAGGTTCTTGTTCAAGCAAAATAGGGTGAGAATGATTGTGGATTGTCATGCCACGCCGGTTCATGTGATCCAGGATGAAGGGCTTATGCAGCCTTTGTGCTTGGTTGGATCAACGCTGCGTGCGCCCCATGGTTGTCATTCGCAGTATATGGCTAATATGGGCTCCATTGCGTCGTTGGCTTTGGCGGTTATCATTAATGGAAATGATGATGAGGCTATTGGTGGACGGAGCTTGATGAGGCTGTGGGGCTTGGTTGTTTGCCATCACACTTCTGCGAGGTGCATTCCGTTTCCTCTTAGGTATGCTTGTGAGTTTCTAATGCAGGCCTTTGGACTACAGTTGCATATGGAATTGCAGTTGGCTTCACAGATGTCGGAGAAACATGTTTTGAGGACTCAGACTCTGTTGTGTGATATGCTTCTTCGCGACACCCCGACTGGGATTATTACTCAAAGTCCTAGTATCATGAATCTTGTCAAGTGTGATGGGGCAGCGCTTTACTACCAAGACAAGTACTACCCAATTGGTGTCACCCCCAGTGAAGCTCAGATCAAGGACATTGTTGAGTGGTTGTTGGCTTCGCATGGAACTTCGACAGGTTTAAGCACTGACAGTTTGGCTGATGCTGGGTACCCTGGAGCTGCCTCACTTGGTGATGCTGTCTGTGGGATGGCTGCTGCTTATATTACTAAAAGGGACTTTCTGTTCTGGTTCCGATCCCACACCGCAAAAGAGGTGAAATGGGGTGGAGCAAAGCATCATCCTGAGGACAAGGATGATGGGCAGAGGATGCATCCACGTTCTTCCTTTAAGGCGTTTTTGGAAGTGGTTAAAAGCCGTAGTTTGTCATGGGACAATGCAGAAATGGATGCCATACATTCCTTGCAAATTATTTTGCGAGACTCATTTAAGGATGCTGAGACAAACAACTTAAAAGCTGTTACACAGGCCCAGCATCGGGATCTGGAATATCAAGGGATTGACGAGCTCAGCTCAGTGGCAAGAGAAATGGTTAGATTGATAGAGACTGCAACTGCTCCCATATTTGCTGTAGATGTTAATGGGTGTATAAATGGGTGGAATGCAAAGGTTGCAGAGCTGACAGGGCTTTCAGTTGAGGAGGCTACAGGGAAATCCTTGGTACATGATCTGGTTTACAAAGAATCTGAAGAAACTGTTGACAAACTTCTCCTCCACGCTCTGAGAGGAGAAGAAGATAAAAATGTAGAGATAAAACTGAGGACATTTGGCCCAGAGCATGAAAACAAGCCTGTTTTTGTGGTGGTTAATGCTTGCTCTAGCAAGGATTACACTAATAATATCGTGGGAGTTTGCTTTGTTGGTCAGGATGTTACTGGTCAAAAAGTGGTAATGGACAAATTCGTAAACATACAAGGTGATTACAAGGCCATTGTTCATAGTCCCAATCCTTTGATCCCGCCCATATTTGCATCGGATGATAACACATGTTGCTCAGAGTGGAACACTGCCATGGAAAAACTCACTGGGTGGAGCCGTGGAGATATCATTGGAAAAGTGTTGGTTGGGGAGGTCTTTGGCAGTTGTTGTCGACTCAAGGGTCCAGATGCTATAACAAAATTCATGATTGTCTTACACAATGCCATTGGAGGCTTAGACACGGACAAGTTTCCTTTTTCATTCTTTGACCGGAATGGGAAATATGTACAAGCTCTCTTGACAGCAAATAAGAGGGTGAATATAGACGGCCAGGTTATTGGAGCTTTCTGCTTTCTGCAGATTCCTAGTTCAGAACTGCAGCAAGCTCTTAGAGTTCAGAGACAACAGGAGAAGCAATGTTTCGCTAGGATGAAAGAATTCGCTTACATATGTCAGGAAATAAAAAATCCTTTAAGTGGTATACGCTTTACTAACTCACTTTTGGAGGCTACAGAGTTAACTGAAGATCAAAAGCAGTTCTTGGAGACTAGTGGTGCTTGTGAGAAGCAAATTTTGAAGATTATAAAGGACGTTGATCTGGCTAGCATTGAAGATGGGTAA

Protein Analysis

963

Amino Acids

106.14

Weight (kDa)

5.73

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 82 - 198 2.2e-37 PAS fold
GAF PF01590 232 - 406 1.2e-15 GAF domain
PHY PF00360 422 - 596 6.1e-57 Phytochrome region
PAS PF00989 627 - 742 3.9e-24 PAS fold
PAS PF00989 758 - 877 8.7e-19 PAS fold
HisKA PF00512 901 - 963 1.3e-13 His Kinase A (phospho-acceptor) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2855
AasI GACNNNNNNGTC 1 cut(s) 2451
Acc36I ACCTGC 1 cut(s) 363
Acc65I GGTACC 1 cut(s) 1503
AccB1I GGYRCC 2 cut(s) 59, 1503
AccI GTMKAC 3 cut(s) 135, 2443, 2752
AccII CGCG 5 cut(s) 347, 349, 351, 545, 1299
AclWI GGATC 6 cut(s) 397, 934, 988, 1584, 1842, 2302
AcoI YGGCCR 4 cut(s) 271, 648, 849, 2599
AcsI RAATTY 4 cut(s) 2252, 2471, 2708, 2841
AcuI CTGAAG 2 cut(s) 2061, 2814
AcyI GRCGYC 1 cut(s) 869
AdeI CACNNNGTG 3 cut(s) 154, 1526, 2382
AfaI GTAC 7 cut(s) 128, 248, 764, 1388, 1505, 2016, 2559
AfeI AGCGCT 1 cut(s) 1368
AfiI CCNNNNNNNGG 7 cut(s) 321, 426, 833, 1311, 1830, 1831, 2747
AflIII ACRYGT 2 cut(s) 1255, 2339
AgsI TTSAA 4 cut(s) 73, 886, 2848, 2882
AhlI ACTAGT 1 cut(s) 2819
AjnI CCWGG 3 cut(s) 942, 1507, 2601
AleI CACNNNNGTG 1 cut(s) 716
AloI GAACNNNNNNTCC 2 cut(s) 1562, 1594
Alw21I GWGCWC 4 cut(s) 296, 390, 786, 1863
Alw26I GTCTC 6 cut(s) 642, 1767, 1787, 1892, 2665, 2810
Alw44I GTGCAC 1 cut(s) 292
AlwI GGATC 6 cut(s) 397, 934, 988, 1584, 1842, 2302
AlwNI CAGNNNCTG 3 cut(s) 646, 679, 1276
Ama87I CYCGRG 2 cut(s) 389, 480
Aor51HI AGCGCT 1 cut(s) 1368
ApaLI GTGCAC 1 cut(s) 292
ApoI RAATTY 4 cut(s) 2252, 2471, 2708, 2841
AseI ATTAAT 1 cut(s) 1068
Asp700I GAANNNNTTC 1 cut(s) 2708
Asp718I GGTACC 1 cut(s) 1503
AspLEI GCGC 6 cut(s) 124, 349, 351, 479, 997, 1369
AspS9I GGNCC 6 cut(s) 417, 604, 627, 1822, 2123, 2453
AsuC2I CCSGG 3 cut(s) 608, 625, 684
AsuHPI GGTGA 7 cut(s) 118, 910, 1396, 1538, 1621, 2282, 2599
AsuNHI GCTAGC 1 cut(s) 2873
AvaI CYCGRG 2 cut(s) 389, 480
AvaII GGWCC 3 cut(s) 417, 604, 2453
AxyI CCTNAGG 3 cut(s) 425, 864, 1638
BaeGI GKGCMC 2 cut(s) 62, 296
BanI GGYRCC 2 cut(s) 59, 1503
BanII GRGCYC 2 cut(s) 1037, 1863
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 4 cut(s) 296, 390, 786, 1863
BccI CCATC 5 cut(s) 1143, 1352, 1537, 1646, 2879
BceAI ACGGC 9 cut(s) 137, 258, 287, 520, 614, 635, 1695, 2373, 2614
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 3 cut(s) 944, 1509, 2603
BciVI GTATCC 1 cut(s) 504
BcnI CCSGG 3 cut(s) 608, 625, 684
BcoDI GTCTC 6 cut(s) 642, 1767, 1787, 1892, 2665, 2810
BcuI ACTAGT 1 cut(s) 2819
BfaI CTAG 7 cut(s) 594, 1332, 2172, 2637, 2697, 2820, 2874
BfmI CTRYAG 9 cut(s) 334, 618, 671, 1208, 1923, 1998, 2627, 2648, 2781
BfoI RGCGCY 2 cut(s) 480, 1370
BfuAI ACCTGC 1 cut(s) 363
BfuI GTATCC 1 cut(s) 504
BglI GCCNNNNNGGC 1 cut(s) 656
BlpI GCTNAGC 1 cut(s) 1862
BmcAI AGTACT 1 cut(s) 1388
Bme1390I CCNGG 6 cut(s) 608, 625, 684, 944, 1509, 2603
Bme18I GGWCC 3 cut(s) 417, 604, 2453
BmeT110I CYCGRG 2 cut(s) 389, 480
BmgT120I GGNCC 6 cut(s) 417, 604, 627, 1822, 2123, 2453
BmrFI CCNGG 6 cut(s) 608, 625, 684, 944, 1509, 2603
BmrI ACTGGG 5 cut(s) 367, 750, 1320, 1404, 2388
BmtI GCTAGC 1 cut(s) 2877
BmuI ACTGGG 5 cut(s) 367, 750, 1320, 1404, 2388
BpiI GAAGAC 1 cut(s) 45
BplI GAGNNNNNCTC 2 cut(s) 1850, 1882
BpmI CTGGAG 2 cut(s) 482, 1530
Bpu1102I GCTNAGC 1 cut(s) 1862
BpuEI CTTGAG 1 cut(s) 2432
BpuMI CCSGG 3 cut(s) 608, 625, 684
BsaHI GRCGYC 1 cut(s) 869
BsaJI CCNNGG 8 cut(s) 303, 624, 852, 1000, 1507, 2010, 2364, 2389
BsaWI WCCGGW 2 cut(s) 382, 2541
BsaXI ACNNNNNCTCC 4 cut(s) 455, 485, 2049, 2079
Bsc4I CCNNNNNNNGG 7 cut(s) 321, 426, 833, 1311, 1830, 1831, 2747
Bse118I RCCGGY 1 cut(s) 928
Bse1I ACTGG 6 cut(s) 373, 745, 1315, 1410, 2233, 2383
Bse21I CCTNAGG 3 cut(s) 425, 864, 1638
Bse3DI GCAATG 3 cut(s) 327, 1038, 2693
BseBI CCWGG 3 cut(s) 944, 1509, 2603
BseDI CCNNGG 8 cut(s) 303, 624, 852, 1000, 1507, 2010, 2364, 2389
BseLI CCNNNNNNNGG 7 cut(s) 321, 426, 833, 1311, 1830, 1831, 2747
BseMI GCAATG 3 cut(s) 327, 1038, 2693
BseNI ACTGG 6 cut(s) 373, 745, 1315, 1410, 2233, 2383
BsePI GCGCGC 1 cut(s) 347
BseRI GAGGAG 4 cut(s) 323, 2006, 2054, 2094
BseSI GKGCMC 2 cut(s) 62, 296
BseYI CCCAGC 3 cut(s) 33, 1499, 1824
BsgI GTGCAG 2 cut(s) 131, 653
Bsh1236I CGCG 5 cut(s) 347, 349, 351, 545, 1299
Bsh1285I CGRYCG 1 cut(s) 135
BshNI GGYRCC 2 cut(s) 59, 1503
BsiEI CGRYCG 1 cut(s) 135
BsiHKAI GWGCWC 4 cut(s) 296, 390, 786, 1863
BsiHKCI CYCGRG 2 cut(s) 389, 480
BslFI GGGAC 4 cut(s) 430, 1583, 1739, 2280
BslI CCNNNNNNNGG 7 cut(s) 321, 426, 833, 1311, 1830, 1831, 2747
BsmAI GTCTC 6 cut(s) 642, 1767, 1787, 1892, 2665, 2810
BsmFI GGGAC 4 cut(s) 430, 1583, 1739, 2280
BsmI GAATGC 2 cut(s) 1155, 1960
BsoBI CYCGRG 2 cut(s) 389, 480
Bsp1286I GDGCHC 6 cut(s) 62, 296, 390, 786, 1037, 1863
Bsp1407I TGTACA 2 cut(s) 762, 2557
Bsp1720I GCTNAGC 1 cut(s) 1862
Bsp19I CCATGG 2 cut(s) 1000, 2364
Bsp68I TCGCGA 1 cut(s) 1299
BspFNI CGCG 5 cut(s) 347, 349, 351, 545, 1299
BspHI TCATGA 3 cut(s) 772, 1338, 2475
BspMAI CTGCAG 3 cut(s) 675, 2631, 2652
BspMI ACCTGC 1 cut(s) 363
BspOI GCTAGC 1 cut(s) 2877
BspPI GGATC 6 cut(s) 397, 934, 988, 1584, 1842, 2302
BspQI GCTCTTC 1 cut(s) 440
BspT107I GGYRCC 2 cut(s) 59, 1503
BsrDI GCAATG 3 cut(s) 327, 1038, 2693
BsrFI RCCGGY 1 cut(s) 928
BsrGI TGTACA 2 cut(s) 762, 2557
BsrI ACTGG 6 cut(s) 373, 745, 1315, 1410, 2233, 2383
BssAI RCCGGY 1 cut(s) 928
BssECI CCNNGG 8 cut(s) 303, 624, 852, 1000, 1507, 2010, 2364, 2389
BssHII GCGCGC 1 cut(s) 347
BssNAI GTATAC 1 cut(s) 2753
BssNI GRCGYC 1 cut(s) 869
BssT1I CCWWGG 3 cut(s) 1000, 2010, 2364
Bst1107I GTATAC 1 cut(s) 2753
Bst2UI CCWGG 3 cut(s) 944, 1509, 2603
Bst4CI ACNGT 5 cut(s) 133, 715, 1212, 1487, 2050
Bst6I CTCTTC 2 cut(s) 440, 498
BstACI GRCGYC 1 cut(s) 869
BstAPI GCANNNNNTGC 3 cut(s) 679, 1663, 1964
BstAUI TGTACA 2 cut(s) 762, 2557
BstDSI CCRYGG 5 cut(s) 303, 852, 1000, 2364, 2389
BstFNI CGCG 5 cut(s) 347, 349, 351, 545, 1299
BstH2I RGCGCY 2 cut(s) 480, 1370
BstHHI GCGC 6 cut(s) 124, 349, 351, 479, 997, 1369
BstMAI GTCTC 6 cut(s) 642, 1767, 1787, 1892, 2665, 2810
BstMCI CGRYCG 1 cut(s) 135
BstNI CCWGG 3 cut(s) 944, 1509, 2603
BstNSI RCATGY 2 cut(s) 1259, 2343
BstSCI CCNGG 6 cut(s) 606, 623, 682, 942, 1507, 2601
BstSFI CTRYAG 9 cut(s) 334, 618, 671, 1208, 1923, 1998, 2627, 2648, 2781
BstSLI GKGCMC 2 cut(s) 62, 296
BstUI CGCG 5 cut(s) 347, 349, 351, 545, 1299
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 2 cut(s) 402, 1834
BstXI CCANNNNNNTGG 1 cut(s) 2609
BstYI RGATCY 2 cut(s) 402, 1834
BstZ17I GTATAC 1 cut(s) 2753
Bsu36I CCTNAGG 3 cut(s) 425, 864, 1638
BsuI GTATCC 1 cut(s) 504
BtgI CCRYGG 5 cut(s) 303, 852, 1000, 2364, 2389
BtsI GCAGTG 2 cut(s) 677, 2358
BtsIMutI CAGTG 6 cut(s) 677, 1417, 1479, 1875, 2358, 2376
BtuMI TCGCGA 1 cut(s) 1299
BveI ACCTGC 1 cut(s) 363
CaiI CAGNNNCTG 3 cut(s) 646, 679, 1276
CciI TCATGA 3 cut(s) 772, 1338, 2475
CfoI GCGC 6 cut(s) 124, 349, 351, 479, 997, 1369
Cfr10I RCCGGY 1 cut(s) 928
Cfr13I GGNCC 6 cut(s) 417, 604, 627, 1822, 2123, 2453
CseI GACGC 4 cut(s) 146, 439, 858, 1032
Csp6I GTAC 7 cut(s) 127, 247, 763, 1387, 1504, 2015, 2558
CviQI GTAC 7 cut(s) 127, 247, 763, 1387, 1504, 2015, 2558
DraIII CACNNNGTG 3 cut(s) 154, 1526, 2382
DrdI GACNNNNNNGTC 1 cut(s) 2451
DseDI GACNNNNNNGTC 1 cut(s) 2451
EaeI YGGCCR 4 cut(s) 271, 648, 849, 2599
Eam1104I CTCTTC 2 cut(s) 440, 498
EarI CTCTTC 2 cut(s) 440, 498
EciI GGCGGA 3 cut(s) 30, 113, 815
Ecl136II GAGCTC 1 cut(s) 1861
Eco130I CCWWGG 3 cut(s) 1000, 2010, 2364
Eco147I AGGCCT 1 cut(s) 1199
Eco24I GRGCYC 2 cut(s) 1037, 1863
Eco32I GATATC 1 cut(s) 2398
Eco47I GGWCC 3 cut(s) 417, 604, 2453
Eco47III AGCGCT 1 cut(s) 1368
Eco53kI GAGCTC 1 cut(s) 1861
Eco57I CTGAAG 2 cut(s) 2061, 2814
Eco81I CCTNAGG 3 cut(s) 425, 864, 1638
Eco88I CYCGRG 2 cut(s) 389, 480
EcoICRI GAGCTC 1 cut(s) 1861
EcoO109I RGGNCCY 1 cut(s) 604
EcoRI GAATTC 1 cut(s) 2708
EcoRII CCWGG 3 cut(s) 942, 1507, 2601
EcoRV GATATC 1 cut(s) 2398
EcoT14I CCWWGG 3 cut(s) 1000, 2010, 2364
EcoT22I ATGCAT 1 cut(s) 1668
EcoT38I GRGCYC 2 cut(s) 1037, 1863
ErhI CCWWGG 3 cut(s) 1000, 2010, 2364
FalI AAGNNNNNCTT 2 cut(s) 2795, 2827
FaqI GGGAC 4 cut(s) 430, 1583, 1739, 2280
FauI CCCGC 2 cut(s) 390, 2319
FauNDI CATATG 2 cut(s) 1218, 2720
FblI GTMKAC 3 cut(s) 135, 2443, 2752
FriOI GRGCYC 2 cut(s) 1037, 1863
FspBI CTAG 7 cut(s) 594, 1332, 2172, 2637, 2697, 2820, 2874
GlaI GCGC 6 cut(s) 123, 348, 350, 478, 996, 1368
GsaI CCCAGC 3 cut(s) 37, 1503, 1828
GsuI CTGGAG 2 cut(s) 482, 1530
HaeII RGCGCY 2 cut(s) 480, 1370
HgaI GACGC 4 cut(s) 146, 439, 858, 1032
HhaI GCGC 6 cut(s) 124, 349, 351, 479, 997, 1369
Hin1I GRCGYC 1 cut(s) 869
Hin6I GCGC 6 cut(s) 122, 347, 349, 477, 995, 1367
HinP1I GCGC 6 cut(s) 122, 347, 349, 477, 995, 1367
HincII GTYRAC 4 cut(s) 136, 2053, 2444, 2790
HindII GTYRAC 4 cut(s) 136, 2053, 2444, 2790
HindIII AAGCTT 1 cut(s) 698
HpaI GTTAAC 1 cut(s) 2790
HphI GGTGA 7 cut(s) 118, 910, 1396, 1538, 1621, 2282, 2599
Hpy99I CGWCG 4 cut(s) 140, 457, 874, 1048
HpyAV CCTTC 6 cut(s) 34, 295, 328, 453, 460, 944
HpyCH4III ACNGT 5 cut(s) 133, 715, 1212, 1487, 2050
HpyCH4IV ACGT 3 cut(s) 455, 1672, 2862
HpySE526I ACGT 3 cut(s) 455, 1672, 2862
Hsp92I GRCGYC 1 cut(s) 869
HspAI GCGC 6 cut(s) 122, 347, 349, 477, 995, 1367
KpnI GGTACC 1 cut(s) 1507
KspAI GTTAAC 1 cut(s) 2790
LguI GCTCTTC 1 cut(s) 440
MaeI CTAG 7 cut(s) 594, 1332, 2172, 2637, 2697, 2820, 2874
MaeII ACGT 3 cut(s) 455, 1672, 2862
MaeIII GTNAC 5 cut(s) 675, 707, 1402, 1813, 2224
MauBI CGCGCGCG 1 cut(s) 347
MfeI CAATTG 1 cut(s) 1395
MflI RGATCY 2 cut(s) 402, 1834
MhlI GDGCHC 6 cut(s) 62, 296, 390, 786, 1037, 1863
MluCI AATT 9 cut(s) 67, 504, 1223, 1395, 1761, 2252, 2471, 2708, 2841
MlyI GAGTC 7 cut(s) 110, 179, 320, 1261, 1267, 1769, 2439
MmeI TCCRAC 2 cut(s) 958, 1227
Mph1103I ATGCAT 1 cut(s) 1668
MroXI GAANNNNTTC 1 cut(s) 2708
MslI CAYNNNNRTG 3 cut(s) 716, 777, 2363
MspA1I CMGCKG 1 cut(s) 670
MspR9I CCNGG 6 cut(s) 608, 625, 684, 944, 1509, 2603
MunI CAATTG 1 cut(s) 1395
Mva1269I GAATGC 2 cut(s) 1155, 1960
MvaI CCWGG 3 cut(s) 944, 1509, 2603
MvnI CGCG 5 cut(s) 347, 349, 351, 545, 1299
NciI CCSGG 3 cut(s) 608, 625, 684
NcoI CCATGG 2 cut(s) 1000, 2364
NdeI CATATG 2 cut(s) 1218, 2720
NheI GCTAGC 1 cut(s) 2873
NmeAIII GCCGAG 1 cut(s) 121
NmuCI GTSAC 3 cut(s) 675, 707, 1402
NruI TCGCGA 1 cut(s) 1299
NsiI ATGCAT 1 cut(s) 1668
NspI RCATGY 2 cut(s) 1259, 2343
OliI CACNNNNGTG 1 cut(s) 716
PaeR7I CTCGAG 1 cut(s) 389
PagI TCATGA 3 cut(s) 772, 1338, 2475
PauI GCGCGC 1 cut(s) 347
PceI AGGCCT 1 cut(s) 1199
PciI ACATGT 2 cut(s) 1255, 2339
PciSI GCTCTTC 1 cut(s) 440
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 165
PctI GAATGC 2 cut(s) 1155, 1960
PdmI GAANNNNTTC 1 cut(s) 2708
PfeI GAWTC 4 cut(s) 218, 1342, 2036, 2632
PfoI TCCNGGA 1 cut(s) 942
PleI GAGTC 7 cut(s) 109, 178, 319, 1261, 1267, 1769, 2439
PpsI GAGTC 7 cut(s) 109, 178, 319, 1261, 1267, 1769, 2439
PpuMI RGGWCCY 1 cut(s) 604
PscI ACATGT 2 cut(s) 1255, 2339
PshBI ATTAAT 1 cut(s) 1068
PsiI TTATAA 1 cut(s) 2855
Psp124BI GAGCTC 1 cut(s) 1863
Psp5II RGGWCCY 1 cut(s) 604
Psp6I CCWGG 3 cut(s) 942, 1507, 2601
PspFI CCCAGC 3 cut(s) 33, 1499, 1824
PspGI CCWGG 3 cut(s) 942, 1507, 2601
PspPI GGNCC 6 cut(s) 417, 604, 627, 1822, 2123, 2453
PspPPI RGGWCCY 1 cut(s) 604
PstI CTGCAG 3 cut(s) 675, 2631, 2652
PstNI CAGNNNCTG 3 cut(s) 646, 679, 1276
PsuI RGATCY 2 cut(s) 402, 1834
PteI GCGCGC 1 cut(s) 347
PvuII CAGCTG 1 cut(s) 670
RruI TCGCGA 1 cut(s) 1299
RsaI GTAC 7 cut(s) 128, 248, 764, 1388, 1505, 2016, 2559
RsaNI GTAC 7 cut(s) 127, 247, 763, 1387, 1504, 2015, 2558
RseI CAYNNNNRTG 3 cut(s) 716, 777, 2363
SacI GAGCTC 1 cut(s) 1863
SalI GTCGAC 2 cut(s) 134, 2442
SapI GCTCTTC 1 cut(s) 440
Sau96I GGNCC 6 cut(s) 417, 604, 627, 1822, 2123, 2453
ScaI AGTACT 1 cut(s) 1388
SchI GAGTC 7 cut(s) 110, 179, 320, 1261, 1267, 1769, 2439
ScrFI CCNGG 6 cut(s) 608, 625, 684, 944, 1509, 2603
SduI GDGCHC 6 cut(s) 62, 296, 390, 786, 1037, 1863
SfcI CTRYAG 9 cut(s) 334, 618, 671, 1208, 1923, 1998, 2627, 2648, 2781
Sfr274I CTCGAG 1 cut(s) 389
SinI GGWCC 3 cut(s) 417, 604, 2453
SlaI CTCGAG 1 cut(s) 389
SmiMI CAYNNNNRTG 3 cut(s) 716, 777, 2363
SmlI CTYRAG 2 cut(s) 389, 2447
SmoI CTYRAG 2 cut(s) 389, 2447
SpeI ACTAGT 1 cut(s) 2819
Sse9I AATT 9 cut(s) 67, 504, 1223, 1395, 1761, 2252, 2471, 2708, 2841
SseBI AGGCCT 1 cut(s) 1199
SspMI CTAG 7 cut(s) 594, 1332, 2172, 2637, 2697, 2820, 2874
SstI GAGCTC 1 cut(s) 1863
StuI AGGCCT 1 cut(s) 1199
StyD4I CCNGG 6 cut(s) 606, 623, 682, 942, 1507, 2601
StyI CCWWGG 3 cut(s) 1000, 2010, 2364
TaaI ACNGT 5 cut(s) 133, 715, 1212, 1487, 2050
TaiI ACGT 3 cut(s) 458, 1675, 2865
TaqII GACCGA 1 cut(s) 434
TasI AATT 9 cut(s) 67, 504, 1223, 1395, 1761, 2252, 2471, 2708, 2841
TatI WGTACW 4 cut(s) 126, 762, 1386, 2557
TauI GCSGC 3 cut(s) 30, 33, 273
TfiI GAWTC 4 cut(s) 218, 1342, 2036, 2632
TscAI CASTG 6 cut(s) 684, 1417, 1486, 1875, 2365, 2383
TseFI GTSAC 3 cut(s) 675, 707, 1402
Tsp45I GTSAC 3 cut(s) 675, 707, 1402
TspDTI ATGAA 9 cut(s) 761, 923, 963, 1355, 2148, 2279, 2464, 2520, 2717
TspGWI ACGGA 5 cut(s) 211, 869, 1114, 1149, 2528
TspRI CASTG 6 cut(s) 684, 1417, 1486, 1875, 2365, 2383
VneI GTGCAC 1 cut(s) 292
VpaK11BI GGWCC 3 cut(s) 417, 604, 2453
VspI ATTAAT 1 cut(s) 1068
XapI RAATTY 4 cut(s) 2252, 2471, 2708, 2841
XceI RCATGY 2 cut(s) 1259, 2343
XcmI CCANNNNNNNNNTGG 1 cut(s) 1045
XhoI CTCGAG 1 cut(s) 389
XmiI GTMKAC 3 cut(s) 135, 2443, 2752
XmnI GAANNNNTTC 1 cut(s) 2708
XspI CTAG 7 cut(s) 594, 1332, 2172, 2637, 2697, 2820, 2874
ZrmI AGTACT 1 cut(s) 1388
Zsp2I ATGCAT 1 cut(s) 1668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.