Rw4G022430

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009857
Physical Location & Seq
Forward (+)
47793009 .. 47798455
5447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G022430.1

Sequence Viewer

Length: 3402 bp
ATGGCTTCAGCAGGCGGAGGCTCCGGCGGCGGCGGGGGGAAGGCTCAGTCGTCGTCGGGCACCAGCAATTTCAAGCAGCACAACAGCAGCACTCCGGCGGAGTCGGTGAGCAAAGCGGTGGCGCAGTACACGGTCGACGCTCGGCTGCACGCCGTGTTCGAGCAGTCCGAGTCCGGCAAGTCTTTCGACTACTCGCAGAGCATGAGAAGCACGAAAGAATCCGTGCCGGAGCAGCAAATCACGGCGTACCTGTCCAAAATCCAGAGGGGCGGGCATATCCAGCCCTTCGGGTGCACGATTGCCGTGGAGGAGTCCACCTTCGGGGTCATTGCCTACAGCGAGAACGCGCGCGACCTGCTCGACCTAATGCCCCAGTCGGTTCCGGTGCTCGAGAGGCGGGAGATCCTCACAATCGGGACCGACCTCAGGACGCTCTTCACGCCTTCCAGCTCGACGTTGCTGGAGAAGGCGTTAGGCGCTCGGGAGATTACTCTTCTCAACCCAATTTGGATACATTCGAGGGTTTCCGGCAAGCCGTTTTACGCGATTTTGCATAGGATTGATGTTGGGGTTGTCATTGATTTGGAGCCTGCTAGAACAGAGGACCCGGCTCTGTCTATAGCCGGGGCCGTGCAGTCTCAGAAGCTGGCCGTGAGGGCGATTTCGCAGCTGCAGTCACTGCCCGGTGGGGATATTAAGCTTTTGTGTGACACGGTGGTGGAGAGTGTGAGGGAGCTGACTGGGTATGATAGAGTTATGGTGTACAAGTTTCATGAGGATGAGCACGGGGAGGTTTTGGCGGAGAGTAAGAGGCCGGATTTGGAGCCTTATCTTGGGCTGCATTATCCGGCCACGGATATACCTCAGGCGTCGAGGTTCTTGTTCAAGCAAAATAGGGTGAGAATGATTGTGGATTGTCATGCCACGCCGGTTCATGTGATCCAGGATGAAGGGCTTATGCAGCCTTTGTGCTTGGTTGGATCAACGCTGCGTGCGCCCCATGGTTGTCATTCGCAGTATATGGCTAATATGGGCTCCATTGCGTCGTTGGCTTTGGCGGTTATCATTAATGGAAATGATGATGAGGCTATTGGTGGACGGAGCTTGATGAGGCTGTGGGGCTTGGTTGTTTGCCATCACACTTCTGCGAGGTGCATTCCGTTTCCTCTTAGGTATGCTTGTGAGTTTCTAATGCAGGCCTTTGGACTACAGTTGCATATGGAATTGCAGTTGGCTTCACAGATGTCGGAGAAACATGTTTTGAGGACTCAGACTCTGTTGTGTGATATGCTTCTTCGCGACACCCCGACTGGGATTATTACTCAAAGTCCTAGTATCATGAATCTTGTCAAGTGTGATGGGGCAGCGCTTTACTACCAAGACAAGTACTACCCAATTGGTGTCACCCCCACTGAAGCTCAGATCAAGGACATTGTTGAGTGGTTGTTGGCTTCGCATGGAACTTCGACAGGTTTAAGCACTGACAGTTTGGCTGATGCTGGGTACCCTGGAGCTGCCTCACTTGGTGATGCTGTCTGTGGGATGGCTGCTGCTTATATTACTAAAAGGGACTTTCTGTTCTGGTTCCGATCCCACACCGCAAAAGAGGTGAAATGGGGTGGAGCAAAGCATCATCCTGAGGACAAGGATGATGGGCAGAGGATGCATCCACGTTCTTCCTTTAAGGCGTTTTTGGAAGTGGTTAAAAGCCGTAGTTTGCCATGGGACAATGCAGAAATGGATGCCATACATTCCTTGCAAATTATTTTGCGAGACTCATTTAAGGATGCTGAGACAAACAACTTAAAAGCTGTTACACAGGCCCAGCATCGGGATCTGGAATATCAAGGGATTGACGAGCTCAGCTCAGTGGCAAGAGAAATGGTTAGATTGATAGAGACTGCAACTGCTCCCATATTTGCTGTAGATGTTAATGGGTGTATAAATGGGTGGAATGCAAAGGTTGCAGAGCTGACAGGGCTTTCAGTTGAGGAGGCTACAGGGAAATCCTTGGTACATGATCTGGTTTACAAAGAATCTGAAGAAACTGTTGACAAACTTCTCCTCCACGCTCTGAGAGGAGAAGAAGATAAAAATGTAGAGATAAAACTGAGGACATTTGGCCCAGAGCATGAAAACAAGCCTGTTTTTGTGGTGGTTAATGCTTGCTCTAGCAAGGATTACACTAATAATATCGTGGGAGTTTGCTTTGTTGGTCAGGATGTTACTGGTCAAAAAGTGGTAATGGACAAATTCGTAAACATACAAGGTGATTACAAGGCCATTGTTCATAGTCCCAATCCTTTGATCCCGCCCATATTTGCATCGGATGATAACACATGTTGCTCAGAGTGGAACACTGCCATGGAAAAACTCACTGGGTGGAGCCGTGGAGATATCATTGGAAAAGTGTTGGTTGGGGAGGTCTTTGGCAGTTGTTGTCGACTCAAGGGTCCAGATGCTATAACAAAATTCATGATTGTCTTGCACAACGCCATTGGAGGCTTAGACACGGACAAGTTTCCTTTTTCATTCTTTGACCGGAATGGGAAATATGTACAAGCTCTCTTGACAGCAAATAAGAGGGTGAATATAGACAGCCAGGTTATTGGAGCTTTCTGCTTTCTGCAGATTCCTAGTTCAGAACTGCAGCAAGCTCTTAGAGTTCAGAGACAACAGGAGAAGCAATGTTTCGCTAGGATGAAAGAATTCGCTTACATATGTCAGGAAATAAAAAATCCTTTAAGTGGTATACGCTTTACTAACTCACTTTTGGAGGCTACAGAGTTAACTGAAGATCAAAAGCAGTTCTTGGAGACTAGTGGTGCTTGTGAGAAGCAAATTTTGAAGATTATAAAGGACGTTGATCTGGCTAGCATTGAAGATGGTTCTTTGGAGCTTGAGAAGGAAGAATTCTTACTTGGAAGTGTCATAAATGCTGTTGTTAGCCAAGTAATGTTATTGCTGAGGGAAAGAAATTTGCAATTGATTCGGGATATTCCAGAAGAAGTCAAAACACTAGCTGTTTATGGTGATCAAGTGAGAATTCAGCAGGTCTTAGCTGATTTCTTATTGAATATGGTACGTTATGCACCATCTGAGGAAGGCTGGGTGGAGATTCATGTTCGTCCAATCATAACTACAATTCCTGATGGACACTCTATGATTCATACAGAATTCAGGTTGGTATGCCCTGGTGAAGGTCTTCCCCCTCAATTAGTTCAAGACATGTTCCATAGTTGTCGATGGATGACTCAGGAGGGCCTAGGACTGAGCATGTGCAGGAAAATTCTAAAGCTCATGAAGGGAGAAGTCCAATATATCAGAGAATCAGAGAGGTGTTATTTCTTAGTTATTCTCGAGCTTCCTATGCCTCAGACACATGCAAAGAGTGTTGACTAG

Protein Analysis

1133

Amino Acids

125.65

Weight (kDa)

5.67

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 82 - 198 2.8e-37 PAS fold
GAF PF01590 232 - 406 1.5e-15 GAF domain
PHY PF00360 422 - 596 1.6e-57 Phytochrome region
PAS PF00989 627 - 742 4.9e-24 PAS fold
PAS PF00989 758 - 877 3.4e-18 PAS fold
HisKA PF00512 901 - 963 1.6e-13 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 1011 - 1123 3.4e-13 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2855
AasI GACNNNNNNGTC 1 cut(s) 2451
Acc36I ACCTGC 2 cut(s) 363, 3043
Acc65I GGTACC 1 cut(s) 1503
AccB1I GGYRCC 2 cut(s) 59, 1503
AccI GTMKAC 3 cut(s) 135, 2443, 2752
AccII CGCG 5 cut(s) 347, 349, 351, 545, 1299
AclWI GGATC 6 cut(s) 397, 934, 988, 1584, 1842, 2302
AcoI YGGCCR 2 cut(s) 648, 849
AcsI RAATTY 9 cut(s) 2252, 2471, 2708, 2841, 2912, 2977, 3045, 3176, 3288
AcuI CTGAAG 3 cut(s) 1434, 2061, 2814
AcyI GRCGYC 1 cut(s) 869
AdeI CACNNNGTG 3 cut(s) 154, 1526, 2382
AfaI GTAC 8 cut(s) 128, 248, 764, 1388, 1505, 2016, 2559, 3084
AfeI AGCGCT 1 cut(s) 1368
AfiI CCNNNNNNNGG 8 cut(s) 321, 426, 833, 1311, 1830, 1831, 2747, 3200
AflIII ACRYGT 3 cut(s) 1255, 2339, 3228
AgsI TTSAA 6 cut(s) 73, 886, 2848, 2882, 3076, 3224
AhlI ACTAGT 1 cut(s) 2819
AjnI CCWGG 4 cut(s) 942, 1507, 2601, 3193
AjuI GAANNNNNNNTTGG 2 cut(s) 2904, 2936
AleI CACNNNNGTG 1 cut(s) 716
AloI GAACNNNNNNTCC 2 cut(s) 1562, 1594
Alw21I GWGCWC 4 cut(s) 296, 390, 786, 1863
Alw26I GTCTC 6 cut(s) 642, 1767, 1787, 1892, 2665, 2810
Alw44I GTGCAC 1 cut(s) 292
AlwI GGATC 6 cut(s) 397, 934, 988, 1584, 1842, 2302
AlwNI CAGNNNCTG 3 cut(s) 646, 679, 1276
Ama87I CYCGRG 3 cut(s) 389, 480, 3359
Aor51HI AGCGCT 1 cut(s) 1368
AoxI GGCC 9 cut(s) 627, 648, 812, 849, 1197, 1821, 2122, 2280, 3262
ApaLI GTGCAC 1 cut(s) 292
ApoI RAATTY 9 cut(s) 2252, 2471, 2708, 2841, 2912, 2977, 3045, 3176, 3288
AseI ATTAAT 1 cut(s) 1068
Asp700I GAANNNNTTC 2 cut(s) 2708, 3204
Asp718I GGTACC 1 cut(s) 1503
AspA2I CCTAGG 1 cut(s) 3265
AspLEI GCGC 6 cut(s) 124, 349, 351, 479, 997, 1369
AspS9I GGNCC 7 cut(s) 417, 604, 627, 1822, 2123, 2453, 3262
AsuC2I CCSGG 3 cut(s) 608, 625, 684
AsuHPI GGTGA 9 cut(s) 118, 910, 1396, 1538, 1621, 2282, 2599, 3044, 3209
AsuNHI GCTAGC 1 cut(s) 2873
AvaI CYCGRG 3 cut(s) 389, 480, 3359
AvaII GGWCC 3 cut(s) 417, 604, 2453
AvrII CCTAGG 1 cut(s) 3265
AxyI CCTNAGG 3 cut(s) 425, 864, 1638
BaeGI GKGCMC 2 cut(s) 62, 296
BanI GGYRCC 2 cut(s) 59, 1503
BanII GRGCYC 2 cut(s) 1037, 1863
BarI GAAGNNNNNNTAC 2 cut(s) 2901, 2933
BbsI GAAGAC 1 cut(s) 3197
Bbv12I GWGCWC 4 cut(s) 296, 390, 786, 1863
BbvCI CCTCAGC 1 cut(s) 2966
BccI CCATC 8 cut(s) 1143, 1352, 1537, 1646, 2879, 3103, 3146, 3240
BceAI ACGGC 8 cut(s) 137, 258, 287, 520, 614, 635, 1695, 2373
BcgI CGANNNNNNTGC 4 cut(s) 166, 200, 2972, 3006
BciT130I CCWGG 4 cut(s) 944, 1509, 2603, 3195
BciVI GTATCC 1 cut(s) 504
BclI TGATCA 1 cut(s) 3034
BcnI CCSGG 3 cut(s) 608, 625, 684
BcoDI GTCTC 6 cut(s) 642, 1767, 1787, 1892, 2665, 2810
BcuI ACTAGT 1 cut(s) 2819
BfmI CTRYAG 9 cut(s) 334, 618, 671, 1208, 1923, 1998, 2627, 2648, 2781
BfoI RGCGCY 2 cut(s) 480, 1370
BfuAI ACCTGC 2 cut(s) 363, 3043
BfuI GTATCC 1 cut(s) 504
BglI GCCNNNNNGGC 1 cut(s) 656
BlnI CCTAGG 1 cut(s) 3265
BlpI GCTNAGC 1 cut(s) 1862
BmcAI AGTACT 1 cut(s) 1388
Bme1390I CCNGG 7 cut(s) 608, 625, 684, 944, 1509, 2603, 3195
Bme18I GGWCC 3 cut(s) 417, 604, 2453
BmeT110I CYCGRG 3 cut(s) 389, 480, 3359
BmgT120I GGNCC 7 cut(s) 417, 604, 627, 1822, 2123, 2453, 3262
BmrFI CCNGG 7 cut(s) 608, 625, 684, 944, 1509, 2603, 3195
BmrI ACTGGG 4 cut(s) 367, 750, 1320, 2388
BmtI GCTAGC 1 cut(s) 2877
BmuI ACTGGG 4 cut(s) 367, 750, 1320, 2388
BpiI GAAGAC 1 cut(s) 3197
BplI GAGNNNNNCTC 2 cut(s) 1850, 1882
BpmI CTGGAG 2 cut(s) 482, 1530
Bpu10I CCTNAGC 1 cut(s) 2966
Bpu1102I GCTNAGC 1 cut(s) 1862
BpuEI CTTGAG 2 cut(s) 2432, 2921
BpuMI CCSGG 3 cut(s) 608, 625, 684
BsaHI GRCGYC 1 cut(s) 869
BsaWI WCCGGW 2 cut(s) 382, 2541
BsaXI ACNNNNNCTCC 4 cut(s) 455, 485, 2049, 2079
Bsc4I CCNNNNNNNGG 8 cut(s) 321, 426, 833, 1311, 1830, 1831, 2747, 3200
Bse118I RCCGGY 1 cut(s) 928
Bse1I ACTGG 5 cut(s) 373, 745, 1315, 2233, 2383
Bse21I CCTNAGG 3 cut(s) 425, 864, 1638
Bse3DI GCAATG 3 cut(s) 327, 1038, 2693
BseBI CCWGG 4 cut(s) 944, 1509, 2603, 3195
BseLI CCNNNNNNNGG 8 cut(s) 321, 426, 833, 1311, 1830, 1831, 2747, 3200
BseMI GCAATG 3 cut(s) 327, 1038, 2693
BseNI ACTGG 5 cut(s) 373, 745, 1315, 2233, 2383
BsePI GCGCGC 1 cut(s) 347
BseRI GAGGAG 4 cut(s) 323, 2006, 2054, 2094
BseSI GKGCMC 2 cut(s) 62, 296
BseYI CCCAGC 3 cut(s) 1499, 1824, 3108
BsgI GTGCAG 3 cut(s) 131, 653, 3301
Bsh1236I CGCG 5 cut(s) 347, 349, 351, 545, 1299
Bsh1285I CGRYCG 1 cut(s) 135
BshFI GGCC 9 cut(s) 629, 650, 814, 851, 1199, 1823, 2124, 2282, 3264
BshNI GGYRCC 2 cut(s) 59, 1503
BsiEI CGRYCG 1 cut(s) 135
BsiHKAI GWGCWC 4 cut(s) 296, 390, 786, 1863
BsiHKCI CYCGRG 3 cut(s) 389, 480, 3359
BslFI GGGAC 4 cut(s) 430, 1583, 1739, 2280
BslI CCNNNNNNNGG 8 cut(s) 321, 426, 833, 1311, 1830, 1831, 2747, 3200
BsmAI GTCTC 6 cut(s) 642, 1767, 1787, 1892, 2665, 2810
BsmFI GGGAC 4 cut(s) 430, 1583, 1739, 2280
BsmI GAATGC 2 cut(s) 1155, 1960
BsnI GGCC 9 cut(s) 629, 650, 814, 851, 1199, 1823, 2124, 2282, 3264
BsoBI CYCGRG 3 cut(s) 389, 480, 3359
Bsp1286I GDGCHC 6 cut(s) 62, 296, 390, 786, 1037, 1863
Bsp1407I TGTACA 2 cut(s) 762, 2557
Bsp1720I GCTNAGC 1 cut(s) 1862
Bsp19I CCATGG 3 cut(s) 1000, 1721, 2364
Bsp68I TCGCGA 1 cut(s) 1299
BspANI GGCC 9 cut(s) 629, 650, 814, 851, 1199, 1823, 2124, 2282, 3264
BspFNI CGCG 5 cut(s) 347, 349, 351, 545, 1299
BspHI TCATGA 4 cut(s) 772, 1338, 2475, 3300
BspMAI CTGCAG 3 cut(s) 675, 2631, 2652
BspMI ACCTGC 2 cut(s) 363, 3043
BspOI GCTAGC 1 cut(s) 2877
BspPI GGATC 6 cut(s) 397, 934, 988, 1584, 1842, 2302
BspQI GCTCTTC 1 cut(s) 440
BspT107I GGYRCC 2 cut(s) 59, 1503
BsrDI GCAATG 3 cut(s) 327, 1038, 2693
BsrFI RCCGGY 1 cut(s) 928
BsrGI TGTACA 2 cut(s) 762, 2557
BsrI ACTGG 5 cut(s) 373, 745, 1315, 2233, 2383
BssAI RCCGGY 1 cut(s) 928
BssHII GCGCGC 1 cut(s) 347
BssNAI GTATAC 1 cut(s) 2753
BssNI GRCGYC 1 cut(s) 869
BssT1I CCWWGG 5 cut(s) 1000, 1721, 2010, 2364, 3265
Bst1107I GTATAC 1 cut(s) 2753
Bst2UI CCWGG 4 cut(s) 944, 1509, 2603, 3195
Bst4CI ACNGT 5 cut(s) 133, 715, 1212, 1487, 2050
Bst6I CTCTTC 2 cut(s) 440, 498
BstACI GRCGYC 1 cut(s) 869
BstAPI GCANNNNNTGC 3 cut(s) 679, 1663, 1964
BstAUI TGTACA 2 cut(s) 762, 2557
BstDSI CCRYGG 6 cut(s) 303, 852, 1000, 1721, 2364, 2389
BstENI CCTNNNNNAGG 1 cut(s) 3198
BstFNI CGCG 5 cut(s) 347, 349, 351, 545, 1299
BstH2I RGCGCY 2 cut(s) 480, 1370
BstHHI GCGC 6 cut(s) 124, 349, 351, 479, 997, 1369
BstMAI GTCTC 6 cut(s) 642, 1767, 1787, 1892, 2665, 2810
BstMCI CGRYCG 1 cut(s) 135
BstNI CCWGG 4 cut(s) 944, 1509, 2603, 3195
BstNSI RCATGY 5 cut(s) 1259, 2343, 3232, 3280, 3386
BstSCI CCNGG 7 cut(s) 606, 623, 682, 942, 1507, 2601, 3193
BstSFI CTRYAG 9 cut(s) 334, 618, 671, 1208, 1923, 1998, 2627, 2648, 2781
BstSLI GKGCMC 2 cut(s) 62, 296
BstUI CGCG 5 cut(s) 347, 349, 351, 545, 1299
BstV2I GAAGAC 1 cut(s) 3197
BstX2I RGATCY 2 cut(s) 402, 1834
BstXI CCANNNNNNTGG 1 cut(s) 2609
BstYI RGATCY 2 cut(s) 402, 1834
BstZ17I GTATAC 1 cut(s) 2753
Bsu36I CCTNAGG 3 cut(s) 425, 864, 1638
BsuI GTATCC 1 cut(s) 504
BsuRI GGCC 9 cut(s) 629, 650, 814, 851, 1199, 1823, 2124, 2282, 3264
BtgI CCRYGG 6 cut(s) 303, 852, 1000, 1721, 2364, 2389
BtsI GCAGTG 2 cut(s) 677, 2358
BtsIMutI CAGTG 6 cut(s) 677, 1410, 1479, 1875, 2358, 2376
BtuMI TCGCGA 1 cut(s) 1299
BveI ACCTGC 2 cut(s) 363, 3043
CaiI CAGNNNCTG 3 cut(s) 646, 679, 1276
CciI TCATGA 4 cut(s) 772, 1338, 2475, 3300
CfoI GCGC 6 cut(s) 124, 349, 351, 479, 997, 1369
Cfr10I RCCGGY 1 cut(s) 928
Cfr13I GGNCC 7 cut(s) 417, 604, 627, 1822, 2123, 2453, 3262
CseI GACGC 4 cut(s) 146, 439, 858, 1032
Csp6I GTAC 8 cut(s) 127, 247, 763, 1387, 1504, 2015, 2558, 3083
CviQI GTAC 8 cut(s) 127, 247, 763, 1387, 1504, 2015, 2558, 3083
DraIII CACNNNGTG 3 cut(s) 154, 1526, 2382
DrdI GACNNNNNNGTC 1 cut(s) 2451
DseDI GACNNNNNNGTC 1 cut(s) 2451
EaeI YGGCCR 2 cut(s) 648, 849
Eam1104I CTCTTC 2 cut(s) 440, 498
EarI CTCTTC 2 cut(s) 440, 498
EciI GGCGGA 3 cut(s) 30, 113, 815
Ecl136II GAGCTC 1 cut(s) 1861
Eco130I CCWWGG 5 cut(s) 1000, 1721, 2010, 2364, 3265
Eco147I AGGCCT 1 cut(s) 1199
Eco24I GRGCYC 2 cut(s) 1037, 1863
Eco32I GATATC 1 cut(s) 2398
Eco47I GGWCC 3 cut(s) 417, 604, 2453
Eco47III AGCGCT 1 cut(s) 1368
Eco53kI GAGCTC 1 cut(s) 1861
Eco57I CTGAAG 3 cut(s) 1434, 2061, 2814
Eco81I CCTNAGG 3 cut(s) 425, 864, 1638
Eco88I CYCGRG 3 cut(s) 389, 480, 3359
EcoICRI GAGCTC 1 cut(s) 1861
EcoNI CCTNNNNNAGG 1 cut(s) 3198
EcoO109I RGGNCCY 2 cut(s) 604, 3262
EcoRI GAATTC 4 cut(s) 2708, 2912, 3045, 3176
EcoRII CCWGG 4 cut(s) 942, 1507, 2601, 3193
EcoRV GATATC 1 cut(s) 2398
EcoT14I CCWWGG 5 cut(s) 1000, 1721, 2010, 2364, 3265
EcoT22I ATGCAT 1 cut(s) 1668
EcoT38I GRGCYC 2 cut(s) 1037, 1863
ErhI CCWWGG 5 cut(s) 1000, 1721, 2010, 2364, 3265
FalI AAGNNNNNCTT 2 cut(s) 2795, 2827
FaqI GGGAC 4 cut(s) 430, 1583, 1739, 2280
FauI CCCGC 4 cut(s) 26, 263, 390, 2319
FauNDI CATATG 2 cut(s) 1218, 2720
FbaI TGATCA 1 cut(s) 3034
FblI GTMKAC 3 cut(s) 135, 2443, 2752
FriOI GRGCYC 2 cut(s) 1037, 1863
GlaI GCGC 6 cut(s) 123, 348, 350, 478, 996, 1368
GsaI CCCAGC 3 cut(s) 1503, 1828, 3112
GsuI CTGGAG 2 cut(s) 482, 1530
HaeII RGCGCY 2 cut(s) 480, 1370
HaeIII GGCC 9 cut(s) 629, 650, 814, 851, 1199, 1823, 2124, 2282, 3264
HgaI GACGC 4 cut(s) 146, 439, 858, 1032
HhaI GCGC 6 cut(s) 124, 349, 351, 479, 997, 1369
Hin1I GRCGYC 1 cut(s) 869
Hin6I GCGC 6 cut(s) 122, 347, 349, 477, 995, 1367
HinP1I GCGC 6 cut(s) 122, 347, 349, 477, 995, 1367
HincII GTYRAC 5 cut(s) 136, 2053, 2444, 2790, 3397
HindII GTYRAC 5 cut(s) 136, 2053, 2444, 2790, 3397
HindIII AAGCTT 1 cut(s) 698
HpaI GTTAAC 1 cut(s) 2790
HphI GGTGA 9 cut(s) 118, 910, 1396, 1538, 1621, 2282, 2599, 3044, 3209
Hpy99I CGWCG 6 cut(s) 55, 58, 140, 457, 874, 1048
HpyCH4III ACNGT 5 cut(s) 133, 715, 1212, 1487, 2050
HpyCH4IV ACGT 4 cut(s) 455, 1672, 2862, 3085
HpySE526I ACGT 4 cut(s) 455, 1672, 2862, 3085
Hsp92I GRCGYC 1 cut(s) 869
HspAI GCGC 6 cut(s) 122, 347, 349, 477, 995, 1367
KpnI GGTACC 1 cut(s) 1507
Ksp22I TGATCA 1 cut(s) 3034
KspAI GTTAAC 1 cut(s) 2790
LguI GCTCTTC 1 cut(s) 440
MaeII ACGT 4 cut(s) 455, 1672, 2862, 3085
MaeIII GTNAC 5 cut(s) 675, 707, 1402, 1813, 2224
MauBI CGCGCGCG 1 cut(s) 347
MfeI CAATTG 2 cut(s) 1395, 2984
MflI RGATCY 2 cut(s) 402, 1834
MhlI GDGCHC 6 cut(s) 62, 296, 390, 786, 1037, 1863
MlyI GAGTC 8 cut(s) 110, 179, 320, 1261, 1267, 1769, 2439, 3247
MmeI TCCRAC 2 cut(s) 958, 1227
Mph1103I ATGCAT 1 cut(s) 1668
MroXI GAANNNNTTC 2 cut(s) 2708, 3204
MslI CAYNNNNRTG 3 cut(s) 716, 777, 2363
MspA1I CMGCKG 1 cut(s) 670
MspR9I CCNGG 7 cut(s) 608, 625, 684, 944, 1509, 2603, 3195
MunI CAATTG 2 cut(s) 1395, 2984
Mva1269I GAATGC 2 cut(s) 1155, 1960
MvaI CCWGG 4 cut(s) 944, 1509, 2603, 3195
MvnI CGCG 5 cut(s) 347, 349, 351, 545, 1299
NciI CCSGG 3 cut(s) 608, 625, 684
NcoI CCATGG 3 cut(s) 1000, 1721, 2364
NdeI CATATG 2 cut(s) 1218, 2720
NheI GCTAGC 1 cut(s) 2873
NmeAIII GCCGAG 1 cut(s) 121
NmuCI GTSAC 3 cut(s) 675, 707, 1402
NruI TCGCGA 1 cut(s) 1299
NsiI ATGCAT 1 cut(s) 1668
NspI RCATGY 5 cut(s) 1259, 2343, 3232, 3280, 3386
OliI CACNNNNGTG 1 cut(s) 716
PaeR7I CTCGAG 2 cut(s) 389, 3359
PagI TCATGA 4 cut(s) 772, 1338, 2475, 3300
PauI GCGCGC 1 cut(s) 347
PceI AGGCCT 1 cut(s) 1199
PciI ACATGT 3 cut(s) 1255, 2339, 3228
PciSI GCTCTTC 1 cut(s) 440
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 165
PctI GAATGC 2 cut(s) 1155, 1960
PdmI GAANNNNTTC 2 cut(s) 2708, 3204
PfeI GAWTC 8 cut(s) 218, 1342, 2036, 2632, 2989, 3118, 3166, 3329
PfoI TCCNGGA 1 cut(s) 942
PleI GAGTC 8 cut(s) 109, 178, 319, 1261, 1267, 1769, 2439, 3247
PpsI GAGTC 8 cut(s) 109, 178, 319, 1261, 1267, 1769, 2439, 3247
PpuMI RGGWCCY 1 cut(s) 604
PscI ACATGT 3 cut(s) 1255, 2339, 3228
PshBI ATTAAT 1 cut(s) 1068
PsiI TTATAA 1 cut(s) 2855
Psp124BI GAGCTC 1 cut(s) 1863
Psp5II RGGWCCY 1 cut(s) 604
Psp6I CCWGG 4 cut(s) 942, 1507, 2601, 3193
PspFI CCCAGC 3 cut(s) 1499, 1824, 3108
PspGI CCWGG 4 cut(s) 942, 1507, 2601, 3193
PspPI GGNCC 7 cut(s) 417, 604, 627, 1822, 2123, 2453, 3262
PspPPI RGGWCCY 1 cut(s) 604
PstI CTGCAG 3 cut(s) 675, 2631, 2652
PstNI CAGNNNCTG 3 cut(s) 646, 679, 1276
PsuI RGATCY 2 cut(s) 402, 1834
PteI GCGCGC 1 cut(s) 347
PvuII CAGCTG 1 cut(s) 670
RruI TCGCGA 1 cut(s) 1299
RsaI GTAC 8 cut(s) 128, 248, 764, 1388, 1505, 2016, 2559, 3084
RsaNI GTAC 8 cut(s) 127, 247, 763, 1387, 1504, 2015, 2558, 3083
RseI CAYNNNNRTG 3 cut(s) 716, 777, 2363
SacI GAGCTC 1 cut(s) 1863
SalI GTCGAC 2 cut(s) 134, 2442
SapI GCTCTTC 1 cut(s) 440
Sau96I GGNCC 7 cut(s) 417, 604, 627, 1822, 2123, 2453, 3262
ScaI AGTACT 1 cut(s) 1388
SchI GAGTC 8 cut(s) 110, 179, 320, 1261, 1267, 1769, 2439, 3247
ScrFI CCNGG 7 cut(s) 608, 625, 684, 944, 1509, 2603, 3195
SduI GDGCHC 6 cut(s) 62, 296, 390, 786, 1037, 1863
SfcI CTRYAG 9 cut(s) 334, 618, 671, 1208, 1923, 1998, 2627, 2648, 2781
Sfr274I CTCGAG 2 cut(s) 389, 3359
SinI GGWCC 3 cut(s) 417, 604, 2453
SlaI CTCGAG 2 cut(s) 389, 3359
SmiMI CAYNNNNRTG 3 cut(s) 716, 777, 2363
SmlI CTYRAG 4 cut(s) 389, 2447, 2900, 3359
SmoI CTYRAG 4 cut(s) 389, 2447, 2900, 3359
SpeI ACTAGT 1 cut(s) 2819
SseBI AGGCCT 1 cut(s) 1199
SstI GAGCTC 1 cut(s) 1863
StuI AGGCCT 1 cut(s) 1199
StyD4I CCNGG 7 cut(s) 606, 623, 682, 942, 1507, 2601, 3193
StyI CCWWGG 5 cut(s) 1000, 1721, 2010, 2364, 3265
TaaI ACNGT 5 cut(s) 133, 715, 1212, 1487, 2050
TaiI ACGT 4 cut(s) 458, 1675, 2865, 3088
TaqII GACCGA 1 cut(s) 434
TatI WGTACW 4 cut(s) 126, 762, 1386, 2557
TauI GCSGC 2 cut(s) 30, 33
TfiI GAWTC 8 cut(s) 218, 1342, 2036, 2632, 2989, 3118, 3166, 3329
TscAI CASTG 6 cut(s) 684, 1417, 1486, 1875, 2365, 2383
TseFI GTSAC 3 cut(s) 675, 707, 1402
Tsp45I GTSAC 3 cut(s) 675, 707, 1402
TspGWI ACGGA 5 cut(s) 211, 869, 1114, 1149, 2528
TspRI CASTG 6 cut(s) 684, 1417, 1486, 1875, 2365, 2383
VneI GTGCAC 1 cut(s) 292
VpaK11BI GGWCC 3 cut(s) 417, 604, 2453
VspI ATTAAT 1 cut(s) 1068
XagI CCTNNNNNAGG 1 cut(s) 3198
XapI RAATTY 9 cut(s) 2252, 2471, 2708, 2841, 2912, 2977, 3045, 3176, 3288
XceI RCATGY 5 cut(s) 1259, 2343, 3232, 3280, 3386
XcmI CCANNNNNNNNNTGG 1 cut(s) 1045
XhoI CTCGAG 2 cut(s) 389, 3359
XmaJI CCTAGG 1 cut(s) 3265
XmiI GTMKAC 3 cut(s) 135, 2443, 2752
XmnI GAANNNNTTC 2 cut(s) 2708, 3204
ZrmI AGTACT 1 cut(s) 1388
Zsp2I ATGCAT 1 cut(s) 1668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.