Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006277

Physical Location & Seq

Genomic Coordinates Reverse (-)

10046287 .. 10049438

Gene Structure 3152 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G18140.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1341 bp

ATGCCAATGCCATTTTTGAAAATGAAGAGCAAGATAAGCAAGGACTCGTTAAGAGAGAAAAGAGTTCTTGGTGTTTGTAAGAAACCTCATAAGGATGTTAAAAATTCATGCTTTGATCCCAAAATATCGGAGAGAATGGAACTTTTGGTCACTGCTACTCAGAATTGCAACAACAACGAGGAACAGAATATAGGTTTGGATTCTTCTACTAGGGATGTTGTAACAAATGAACCCCAAGACCTGCTAGTTGAAGACAGTTCCAAAAATGCGTCGAATATGGATACAATATTTTCACCTGTTCTTGATGATGAACTGTATTCTGAAACTGACCGAGTATTTGTGGAAGGGAACAATGTGCAATGGGAAATGCCTAGGTGGGGAGCTGATGACTGCAGCAACAATCAGTTTTGCAACGATTCTGATTACTTTCTATCTGATGTGCTGATTGCAAGCTTGCCATTCTATGAGAGTGGAAATGTTGATTTTTTCACTGAGATAAGTCCCTTGCCTCATTGTATCTTTCCAGAGCCAAGTGTGTTACTAGATGCAGCTGAGCAGTACATGTTATTACCTTACTTAGAAGATAGATCAGCCAGCAGTGATGATGTTAAGTCTGATGAGGATAACAGGATTAATCGGTCAAGATCAAAAAACTTGGAAGCAGCAGAGAATCACACTGAAGCTGAACAAACTGAAGATTTTGATCCACAGATTTTCTTAAGAAATCAACCAGAATTAGCTGATGTGGTATTCAATTATTTCCCGGATATGCAGCAGCCTAGGGACTCTCCGAAAAGGAAGGCGGTAACCCTTGTTCTTGATTTGGATGAAACTCTGGTCCATTCGACTCTGGAAGTCTGTAGAGATACAGACTTTTCCTTCAGAGTCACTTTCAATATGCAAGAGAACACTGTTTACGTGAAACAAAGACCATACCTTTATAGGTTCTTAGAGAGGGTGGTTGAACTTTTTCATGTTGTCATCTTCACGGCTAGTCACAGCATCTATGCGTCTCAGCTTCTAGATATATTGGATCCAGATGGGAAGTTTGTATCGCAACGTTTTTACCGGGATTCATGCATTCTTTCAGATGGTATTTATACCAAGGATTTAACCGTTTTGGGTCTTGATCTAGCAAAAGTGGCTATAGTTGACAACTGTCCACAGGTGTACAGATTGCAAATAAACAATGGGATCCCTATTAAAAGTTGGTATGATGATCCAACTGATGATGGTCTGATTACATTACTTCCCTTCTTAGAGACATTGGCTGATGCCAACGATGTTCGTCCTGTCATTGCCAAGAGATTCGATCATTCAACAAGAAGTTCTTTTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0036211 GO:0042578 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NIF

Protein Analysis

446

Amino Acids

51.17

Weight (kDa)

4.54

Isoelectric Point (pI)

47.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	270 - 430	1.6e-53	NLI interacting factor-like phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006277)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18140 AT4G18140 AT4G18140 AT4G18140 AT4G18140 AT4G18140 AT5G46410 AT5G46410 AT5G46410
fragaria_vesca	FvH4_3g06370
malus_domestica	MD05G1291500.v1.1 MD10G1267500.v1.1
prunus_persica	Prupe.4G074000_v2.0.a1 Prupe.4G074000_v2.0.a1 Prupe.4G074000_v2.0.a1
pyrus_communis	pycom05g26800 pycom10g22350
rosa_chinensis	RchiOBHm_Chr5g0012931
rosa_laevigata	RLG00000031970
rosa_multiflora	Rmu_sc0006885.1_g000005
rosa_roxburghii	Rroxscaffold_1G00063650
rosa_rugosa	Rorug05G0005400
rosa_samantha	Rh5AG099600 Rh5BG096300 Rh5CG108500 Rh5DG094900
rosa_wichuraiana	Rw5G008700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	249
AciI	CCGC	1 cut(s)	803
AclI	AACGTT	1 cut(s)	1060
AclWI	GGATC	7 cut(s)	110, 698, 1028, 1041, 1189, 1202, 1214
AcsI	RAATTY	1 cut(s)	103
AcuI	CTGAAG	3 cut(s)	699, 714, 865
AfaI	GTAC	2 cut(s)	560, 1172
AfiI	CCNNNNNNNGG	1 cut(s)	377
AflII	CTTAAG	1 cut(s)	718
AflIII	ACRYGT	1 cut(s)	561
AgsI	TTSAA	6 cut(s)	19, 251, 754, 895, 965, 1320
AjuI	GAANNNNNNNTTGG	2 cut(s)	179, 211
AluBI	AGCT	6 cut(s)	383, 453, 551, 683, 740, 1018
AluI	AGCT	6 cut(s)	383, 453, 551, 683, 740, 1018
Alw26I	GTCTC	2 cut(s)	1017, 1256
AlwI	GGATC	7 cut(s)	110, 698, 1028, 1041, 1189, 1202, 1214
ApeKI	GCWGC	5 cut(s)	393, 548, 662, 772, 775
ApoI	RAATTY	1 cut(s)	103
ArsI	GACNNNNNNTTYG	2 cut(s)	320, 352
AseI	ATTAAT	1 cut(s)	633
Asp700I	GAANNNNTTC	1 cut(s)	969
AspA2I	CCTAGG	2 cut(s)	371, 779
AspS9I	GGNCC	1 cut(s)	838
AsuC2I	CCSGG	2 cut(s)	764, 1070
AsuHPI	GGTGA	1 cut(s)	285
AvaII	GGWCC	1 cut(s)	838
AvrII	CCTAGG	2 cut(s)	371, 779
BamHI	GGATCC	2 cut(s)	1033, 1194
BbsI	GAAGAC	1 cut(s)	258
BbvI	GCAGC	5 cut(s)	405, 560, 674, 784, 787
BccI	CCATC	3 cut(s)	1034, 1085, 1226
BceAI	ACGGC	1 cut(s)	1005
BciVI	GTATCC	1 cut(s)	274
BcnI	CCSGG	2 cut(s)	764, 1070
BcoDI	GTCTC	2 cut(s)	1017, 1256
BfaI	CTAG	8 cut(s)	210, 245, 372, 542, 780, 993, 1022, 1133
BfmI	CTRYAG	3 cut(s)	391, 859, 1146
BfrI	CTTAAG	1 cut(s)	718
BfuAI	ACCTGC	1 cut(s)	249
BfuI	GTATCC	1 cut(s)	274
BisI	GCNGC	5 cut(s)	394, 549, 663, 773, 776
BlnI	CCTAGG	2 cut(s)	371, 779
BlpI	GCTNAGC	1 cut(s)	552
BlsI	GCNGC	5 cut(s)	395, 550, 664, 774, 777
Bme1390I	CCNGG	2 cut(s)	764, 1070
Bme18I	GGWCC	1 cut(s)	838
BmgT120I	GGNCC	1 cut(s)	838
BmiI	GGNNCC	2 cut(s)	1035, 1196
BmrFI	CCNGG	2 cut(s)	764, 1070
BmsI	GCATC	3 cut(s)	535, 1011, 1264
BoxI	GACNNNNGTC	1 cut(s)	1158
BpiI	GAAGAC	1 cut(s)	258
Bpu1102I	GCTNAGC	1 cut(s)	552
BpuMI	CCSGG	2 cut(s)	764, 1070
BsaAI	YACGTR	1 cut(s)	919
BsaBI	GATNNNNATC	1 cut(s)	702
BsaJI	CCNNGG	3 cut(s)	371, 779, 1104
Bsc4I	CCNNNNNNNGG	1 cut(s)	377
Bse3DI	GCAATG	2 cut(s)	365, 1296
Bse8I	GATNNNNATC	1 cut(s)	702
BseDI	CCNNGG	3 cut(s)	371, 779, 1104
BseGI	GGATG	3 cut(s)	100, 220, 832
BseJI	GATNNNNATC	1 cut(s)	702
BseLI	CCNNNNNNNGG	1 cut(s)	377
BseMI	GCAATG	2 cut(s)	365, 1296
BseMII	CTCAG	4 cut(s)	173, 483, 543, 1028
BseXI	GCAGC	5 cut(s)	405, 560, 674, 784, 787
BsiSI	CCGG	2 cut(s)	764, 1069
BslFI	GGGAC	2 cut(s)	486, 797
BslI	CCNNNNNNNGG	1 cut(s)	377
BsmAI	GTCTC	2 cut(s)	1017, 1256
BsmBI	CGTCTC	1 cut(s)	1017
BsmFI	GGGAC	2 cut(s)	486, 797
BsmI	GAATGC	1 cut(s)	1080
Bsp1407I	TGTACA	1 cut(s)	1170
Bsp143I	GATC	9 cut(s)	115, 587, 644, 703, 1033, 1129, 1194, 1219, 1312
Bsp1720I	GCTNAGC	1 cut(s)	552
BspACI	CCGC	1 cut(s)	803
BspCNI	CTCAG	4 cut(s)	172, 484, 544, 1027
BspLI	GGNNCC	2 cut(s)	1035, 1196
BspMAI	CTGCAG	1 cut(s)	395
BspMI	ACCTGC	1 cut(s)	249
BspPI	GGATC	7 cut(s)	110, 698, 1028, 1041, 1189, 1202, 1214
BspQI	GCTCTTC	1 cut(s)	20
BspTI	CTTAAG	1 cut(s)	718
BsrDI	GCAATG	2 cut(s)	365, 1296
BsrGI	TGTACA	1 cut(s)	1170
BssECI	CCNNGG	3 cut(s)	371, 779, 1104
BssMI	GATC	9 cut(s)	115, 587, 644, 703, 1033, 1129, 1194, 1219, 1312
BssT1I	CCWWGG	3 cut(s)	371, 779, 1104
Bst4CI	ACNGT	5 cut(s)	257, 315, 913, 1117, 1160
Bst6I	CTCTTC	1 cut(s)	20
BstAFI	CTTAAG	1 cut(s)	718
BstAUI	TGTACA	1 cut(s)	1170
BstBAI	YACGTR	1 cut(s)	919
BstC8I	GCNNGC	3 cut(s)	451, 455, 595
BstDEI	CTNAG	7 cut(s)	159, 492, 552, 577, 949, 1014, 1258
BstEII	GGTNACC	1 cut(s)	805
BstF5I	GGATG	3 cut(s)	100, 220, 832
BstKTI	GATC	9 cut(s)	118, 590, 647, 706, 1036, 1132, 1197, 1222, 1315
BstMAI	GTCTC	2 cut(s)	1017, 1256
BstMBI	GATC	9 cut(s)	115, 587, 644, 703, 1033, 1129, 1194, 1219, 1312
BstMWI	GCNNNNNNNGC	2 cut(s)	36, 1142
BstNSI	RCATGY	1 cut(s)	565
BstPAI	GACNNNNGTC	1 cut(s)	1158
BstPI	GGTNACC	1 cut(s)	805
BstSCI	CCNGG	2 cut(s)	762, 1068
BstSFI	CTRYAG	3 cut(s)	391, 859, 1146
BstV1I	GCAGC	5 cut(s)	405, 560, 674, 784, 787
BstV2I	GAAGAC	1 cut(s)	258
BstX2I	RGATCY	2 cut(s)	1033, 1194
BstYI	RGATCY	2 cut(s)	1033, 1194
BsuI	GTATCC	1 cut(s)	274
BtsCI	GGATG	3 cut(s)	100, 220, 832
BtsI	GCAGTG	2 cut(s)	150, 604
BtsIMutI	CAGTG	5 cut(s)	150, 489, 604, 675, 909
BveI	ACCTGC	1 cut(s)	249
Cac8I	GCNNGC	3 cut(s)	451, 455, 595
Cfr13I	GGNCC	1 cut(s)	838
CseI	GACGC	2 cut(s)	258, 999
Csp6I	GTAC	2 cut(s)	559, 1171
CviAII	CATG	4 cut(s)	108, 562, 974, 1077
CviQI	GTAC	2 cut(s)	559, 1171
DdeI	CTNAG	7 cut(s)	159, 492, 552, 577, 949, 1014, 1258
DpnI	GATC	9 cut(s)	117, 589, 646, 705, 1035, 1131, 1196, 1221, 1314
DpnII	GATC	9 cut(s)	115, 587, 644, 703, 1033, 1129, 1194, 1219, 1312
Eam1104I	CTCTTC	1 cut(s)	20
EarI	CTCTTC	1 cut(s)	20
Eco130I	CCWWGG	3 cut(s)	371, 779, 1104
Eco47I	GGWCC	1 cut(s)	838
Eco57I	CTGAAG	3 cut(s)	699, 714, 865
Eco91I	GGTNACC	1 cut(s)	805
EcoO65I	GGTNACC	1 cut(s)	805
EcoT14I	CCWWGG	3 cut(s)	371, 779, 1104
EcoT22I	ATGCAT	1 cut(s)	1082
ErhI	CCWWGG	3 cut(s)	371, 779, 1104
Esp3I	CGTCTC	1 cut(s)	1017
FaeI	CATG	4 cut(s)	111, 565, 977, 1080
FalI	AAGNNNNNCTT	1 cut(s)	1315
FaqI	GGGAC	2 cut(s)	486, 797
FatI	CATG	4 cut(s)	107, 561, 973, 1076
Fnu4HI	GCNGC	5 cut(s)	394, 549, 663, 773, 776
FokI	GGATG	3 cut(s)	107, 227, 839
Fsp4HI	GCNGC	5 cut(s)	394, 549, 663, 773, 776
FspBI	CTAG	8 cut(s)	210, 245, 372, 542, 780, 993, 1022, 1133
GluI	GCNGC	5 cut(s)	394, 549, 663, 773, 776
HapII	CCGG	2 cut(s)	764, 1069
HgaI	GACGC	2 cut(s)	258, 999
Hin1II	CATG	4 cut(s)	111, 565, 977, 1080
HincII	GTYRAC	1 cut(s)	1153
HindII	GTYRAC	1 cut(s)	1153
HindIII	AAGCTT	1 cut(s)	451
HinfI	GANTC	9 cut(s)	44, 200, 416, 670, 785, 847, 885, 1073, 1308
HpaII	CCGG	2 cut(s)	764, 1069
HphI	GGTGA	1 cut(s)	285
Hpy166II	GTNNAC	4 cut(s)	916, 1153, 1163, 1171
Hpy188III	TCNNGA	8 cut(s)	302, 524, 642, 818, 851, 1022, 1037, 1127
Hpy8I	GTNNAC	4 cut(s)	916, 1153, 1163, 1171
Hpy99I	CGWCG	1 cut(s)	274
HpyAV	CCTTC	4 cut(s)	338, 793, 889, 1264
HpyCH4III	ACNGT	5 cut(s)	257, 315, 913, 1117, 1160
HpyCH4IV	ACGT	2 cut(s)	918, 1060
HpyF10VI	GCNNNNNNNGC	2 cut(s)	36, 1142
HpyF3I	CTNAG	7 cut(s)	159, 492, 552, 577, 949, 1014, 1258
HpySE526I	ACGT	2 cut(s)	918, 1060
Hsp92II	CATG	4 cut(s)	111, 565, 977, 1080
Kzo9I	GATC	9 cut(s)	115, 587, 644, 703, 1033, 1129, 1194, 1219, 1312
LguI	GCTCTTC	1 cut(s)	20
LmnI	GCTCC	1 cut(s)	380
Lsp1109I	GCAGC	5 cut(s)	405, 560, 674, 784, 787
LweI	GCATC	3 cut(s)	535, 1011, 1264
MaeI	CTAG	8 cut(s)	210, 245, 372, 542, 780, 993, 1022, 1133
MaeII	ACGT	2 cut(s)	918, 1060
MaeIII	GTNAC	6 cut(s)	148, 220, 537, 805, 886, 995
MalI	GATC	9 cut(s)	117, 589, 646, 705, 1035, 1131, 1196, 1221, 1314
MboI	GATC	9 cut(s)	115, 587, 644, 703, 1033, 1129, 1194, 1219, 1312
MboII	GAAGA	6 cut(s)	37, 195, 263, 593, 707, 976
MflI	RGATCY	2 cut(s)	1033, 1194
MluCI	AATT	4 cut(s)	103, 163, 734, 754
MlyI	GAGTC	4 cut(s)	38, 779, 841, 894
MmeI	TCCRAC	1 cut(s)	1247
MnlI	CCTC	5 cut(s)	96, 172, 519, 613, 948
Mph1103I	ATGCAT	1 cut(s)	1082
MroXI	GAANNNNTTC	1 cut(s)	969
MseI	TTAA	7 cut(s)	50, 99, 609, 633, 719, 1112, 1203
MslI	CAYNNNNRTG	1 cut(s)	93
MspA1I	CMGCKG	1 cut(s)	551
MspCI	CTTAAG	1 cut(s)	718
MspI	CCGG	2 cut(s)	764, 1069
MspR9I	CCNGG	2 cut(s)	764, 1070
Mva1269I	GAATGC	1 cut(s)	1080
MwoI	GCNNNNNNNGC	2 cut(s)	36, 1142
NciI	CCSGG	2 cut(s)	764, 1070
NdeII	GATC	9 cut(s)	115, 587, 644, 703, 1033, 1129, 1194, 1219, 1312
NlaIII	CATG	4 cut(s)	111, 565, 977, 1080
NlaIV	GGNNCC	2 cut(s)	1035, 1196
NmuCI	GTSAC	3 cut(s)	148, 886, 995
NsiI	ATGCAT	1 cut(s)	1082
NspI	RCATGY	1 cut(s)	565
PciI	ACATGT	1 cut(s)	561
PciSI	GCTCTTC	1 cut(s)	20
PctI	GAATGC	1 cut(s)	1080
PdmI	GAANNNNTTC	1 cut(s)	969
PfeI	GAWTC	5 cut(s)	200, 416, 670, 1073, 1308
PfoI	TCCNGGA	1 cut(s)	762
PkrI	GCNGC	5 cut(s)	395, 550, 664, 774, 777
PleI	GAGTC	4 cut(s)	38, 779, 841, 893
PpsI	GAGTC	4 cut(s)	38, 779, 841, 893
Ppu21I	YACGTR	1 cut(s)	919
PscI	ACATGT	1 cut(s)	561
PshAI	GACNNNNGTC	1 cut(s)	1158
PshBI	ATTAAT	1 cut(s)	633
Psp1406I	AACGTT	1 cut(s)	1060
PspEI	GGTNACC	1 cut(s)	805
PspN4I	GGNNCC	2 cut(s)	1035, 1196
PspPI	GGNCC	1 cut(s)	838
PsrI	GAACNNNNNNTAC	4 cut(s)	798, 830, 899, 931
PstI	CTGCAG	1 cut(s)	395
PsuI	RGATCY	2 cut(s)	1033, 1194
PvuII	CAGCTG	1 cut(s)	551
RsaI	GTAC	2 cut(s)	560, 1172
RsaNI	GTAC	2 cut(s)	559, 1171
RseI	CAYNNNNRTG	1 cut(s)	93
SapI	GCTCTTC	1 cut(s)	20
SaqAI	TTAA	7 cut(s)	50, 99, 609, 633, 719, 1112, 1203
SatI	GCNGC	5 cut(s)	394, 549, 663, 773, 776
Sau3AI	GATC	9 cut(s)	115, 587, 644, 703, 1033, 1129, 1194, 1219, 1312
Sau96I	GGNCC	1 cut(s)	838
SchI	GAGTC	4 cut(s)	38, 779, 841, 894
ScrFI	CCNGG	2 cut(s)	764, 1070
SfaNI	GCATC	3 cut(s)	535, 1011, 1264
SfcI	CTRYAG	3 cut(s)	391, 859, 1146
SinI	GGWCC	1 cut(s)	838
SmiMI	CAYNNNNRTG	1 cut(s)	93
SmlI	CTYRAG	1 cut(s)	718
SmoI	CTYRAG	1 cut(s)	718
Sse9I	AATT	4 cut(s)	103, 163, 734, 754
SsiI	CCGC	1 cut(s)	803
SspI	AATATT	1 cut(s)	288
SspMI	CTAG	8 cut(s)	210, 245, 372, 542, 780, 993, 1022, 1133
StyD4I	CCNGG	2 cut(s)	762, 1068
StyI	CCWWGG	3 cut(s)	371, 779, 1104
TaaI	ACNGT	5 cut(s)	257, 315, 913, 1117, 1160
TaiI	ACGT	2 cut(s)	921, 1063
TaqI	TCGA	3 cut(s)	272, 845, 1311
TaqII	GACCGA	2 cut(s)	345, 627
TasI	AATT	4 cut(s)	103, 163, 734, 754
TatI	WGTACW	2 cut(s)	558, 1170
TfiI	GAWTC	5 cut(s)	200, 416, 670, 1073, 1308
Tru1I	TTAA	7 cut(s)	50, 99, 609, 633, 719, 1112, 1203
Tru9I	TTAA	7 cut(s)	50, 99, 609, 633, 719, 1112, 1203
TscAI	CASTG	5 cut(s)	157, 496, 604, 682, 916
TseFI	GTSAC	3 cut(s)	148, 886, 995
TseI	GCWGC	5 cut(s)	393, 548, 662, 772, 775
Tsp45I	GTSAC	3 cut(s)	148, 886, 995
TspDTI	ATGAA	7 cut(s)	38, 96, 243, 324, 843, 962, 1065
TspRI	CASTG	5 cut(s)	157, 496, 604, 682, 916
Vha464I	CTTAAG	1 cut(s)	718
VpaK11BI	GGWCC	1 cut(s)	838
VspI	ATTAAT	1 cut(s)	633
XapI	RAATTY	1 cut(s)	103
XbaI	TCTAGA	1 cut(s)	1021
XceI	RCATGY	1 cut(s)	565
XmaJI	CCTAGG	2 cut(s)	371, 779
XmnI	GAANNNNTTC	1 cut(s)	969
XspI	CTAG	8 cut(s)	210, 245, 372, 542, 780, 993, 1022, 1133
Zsp2I	ATGCAT	1 cut(s)	1082

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.