AT5G46410

Encodes a SCP1-like small phosphatase (SSP). Three SSPs form a unique group with long N-terminal extensions AT5G46410 (SSP4), AT5G11860 (SSP5), AT4G18140 (SSP4b). SSP4 and SSP4b were localized exclusively in the nuclei, whereas SSP5 accumulated in both nuclei and cytoplasm. All three SSPs encodes active CTD phosphatases like animal SCP1 family proteins, with distinct substrate specificities SSP4 and SSP4b could dephosphorylate both Ser2-PO(4) and Ser5-PO(4) of CTD, whereas SSP5 dephosphorylated only Ser5-PO(4)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006277
Physical Location & Seq
Forward (+)
18825100 .. 18829624
4525 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46410.1

Sequence Viewer

Length: 1362 bp
ATGCCATATATAGAAATGAAGAGCAAGATAAGCAAGGTTCTTGGTGTCTGTAAGAAACATAAAGCAAGTACAAGAAATTCTTGCTCTTTGTCCAAAATATCAGGAGACTTGGAAAGCATAACCGCAGCTATTACTACTTGCCACGACGATGAGAAACAGCACCCTGGGTTGGATACTTTTTCTCGGGAGAGTGAAACTAATGAGCCCGCTGAAATGCTTGTTGAAGATACTTCTCAGTCTCAGGGGTTTGCACCTTGGGTCGATGGTTCTCAATCTGTCGAAAATATGGAAAATGCTTGCAATCACATGTCAAACTCAGATACAATATTTTCACCTGTTCTGAATGATGAACTGGATGGAACTGGTCGAGTATTTACAGCAGGAAATTCTGTGATCTGGGAAACCCCCAGGTGGGGAGCCGACGAGAGCAGCAACAAGATTTGCTTTGACAACCAAACGTGCAACGTTTCTGATTTCTATATTTCTGACGTTCTTATTGCGAGCTTGCCGTTTGATGAGAGTGGAAACAACGATGCGTTCACTGAAATAAGCCCCTTGCCTCATTACATCTTTCCTGAGCAGTACATGGTACTACCTTACTTAGAAGATGGATCAGCCAACAAAGATGATATTAAGTCAGATACTGACAAGATTAATCTCGATAACCATGACTTGTTTCTGGCATTCAACCGGACAAGATCGTACAATGTGGAACCAGATGATCGTGCTGAATCTGAAGTAGCTGAAGACTTTGATCCTCAGCTTTTTATAAAAAATCAGCCGGAATTATCGGATGTTGTATCCAATTATTGGCCTCGGGATACCCTAAGAAAGAAGTCTGTGACCCTTGTCCTTGATTTGGATGAAACTTTGGTCCATTCGACTCTGGAATCGTGTAATGTTGCAGATTTTTCCTTCAGAGTCTTTTTCAACATGCAAGAAAACACGGTCTATGTGAGACAAAGGCCACACCTTTACAGGTTCTTGGAGAGGGTGGGAGAGTTGTTTCATGTCGTTATCTTCACAGCAAGCCACAGCATCTATGCCTCACAACTTCTAGATATACTGGACCCAGATGGAAAGTTCATATCACAGCGTTTTTATCGCGATTCATGCATTCTTTTGGATGGAATTTACACCAAGGATTTAACTGTTCTGGGTCTTGATCTGGCCAAAGTGGCTATAATTGACAACTGTCCGCAGGTGTACAGATTGCAAATAAATAATGGGATCCCTATCAAAAGTTGGTACGATGATCCAACTGATGATGGGTTGATTACTATACTTCCCTTCTTAGAGACTTTGGCTGTTGCTGATGATGTTCGGCCTATCATTGGCAGGAGATTTGGTAACAAGGAATAA

Protein Analysis

453

Amino Acids

51.28

Weight (kDa)

4.55

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 282 - 442 1.2e-51 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 770
AarI CACCTGC 1 cut(s) 1192
Acc36I ACCTGC 1 cut(s) 1192
AccB7I CCANNNNNTGG 1 cut(s) 810
AccII CGCG 1 cut(s) 1107
AciI CCGC 3 cut(s) 123, 207, 1199
AclI AACGTT 1 cut(s) 465
AclWI GGATC 5 cut(s) 619, 749, 1225, 1238, 1250
AcoI YGGCCR 1 cut(s) 1170
AcsI RAATTY 3 cut(s) 76, 385, 1131
AcuI CTGAAG 3 cut(s) 756, 765, 901
AfaI GTAC 6 cut(s) 70, 584, 591, 704, 1208, 1250
AfiI CCNNNNNNNGG 7 cut(s) 169, 411, 412, 413, 810, 859, 1334
AflIII ACRYGT 1 cut(s) 306
AgsI TTSAA 3 cut(s) 224, 688, 931
AjnI CCWGG 2 cut(s) 163, 407
AluBI AGCT 4 cut(s) 128, 504, 743, 763
AluI AGCT 4 cut(s) 128, 504, 743, 763
Alw26I GTCTC 4 cut(s) 99, 243, 952, 1292
AlwI GGATC 5 cut(s) 619, 749, 1225, 1238, 1250
AlwNI CAGNNNCTG 1 cut(s) 644
Ama87I CYCGRG 2 cut(s) 183, 816
AoxI GGCC 4 cut(s) 812, 965, 1170, 1325
ApeKI GCWGC 2 cut(s) 125, 429
ApoI RAATTY 3 cut(s) 76, 385, 1131
AseI ATTAAT 1 cut(s) 654
AspS9I GGNCC 2 cut(s) 874, 1069
AsuHPI GGTGA 1 cut(s) 324
AvaI CYCGRG 2 cut(s) 183, 816
AvaII GGWCC 2 cut(s) 874, 1069
BalI TGGCCA 1 cut(s) 1172
BamHI GGATCC 1 cut(s) 1230
BanII GRGCYC 1 cut(s) 207
BbsI GAAGAC 1 cut(s) 753
BbvCI CCTCAGC 1 cut(s) 759
BbvI GCAGC 2 cut(s) 137, 441
BccI CCATC 6 cut(s) 257, 350, 602, 1070, 1121, 1262
BceAI ACGGC 1 cut(s) 493
BciT130I CCWGG 2 cut(s) 165, 409
BciVI GTATCC 3 cut(s) 166, 811, 814
BcoDI GTCTC 4 cut(s) 99, 243, 952, 1292
BfaI CTAG 1 cut(s) 1058
BfuAI ACCTGC 1 cut(s) 1192
BfuI GTATCC 3 cut(s) 166, 811, 814
BglI GCCNNNNNGGC 1 cut(s) 1178
BisI GCNGC 2 cut(s) 126, 430
BlsI GCNGC 2 cut(s) 127, 431
Bme1390I CCNGG 2 cut(s) 165, 409
Bme18I GGWCC 2 cut(s) 874, 1069
BmeT110I CYCGRG 2 cut(s) 183, 816
BmgT120I GGNCC 2 cut(s) 874, 1069
BmiI GGNNCC 4 cut(s) 418, 714, 1071, 1232
BmrFI CCNGG 2 cut(s) 165, 409
BmsI GCATC 2 cut(s) 523, 1047
BoxI GACNNNNGTC 2 cut(s) 848, 1194
BpiI GAAGAC 1 cut(s) 753
Bpu10I CCTNAGC 2 cut(s) 576, 759
BsaBI GATNNNNATC 1 cut(s) 1235
BsaJI CCNNGG 6 cut(s) 163, 164, 254, 407, 815, 1140
BsaWI WCCGGW 1 cut(s) 690
BsaXI ACNNNNNCTCC 1 cut(s) 1333
Bsc4I CCNNNNNNNGG 7 cut(s) 169, 411, 412, 413, 810, 859, 1334
Bse1I ACTGG 3 cut(s) 357, 367, 1071
Bse8I GATNNNNATC 1 cut(s) 1235
BseBI CCWGG 2 cut(s) 165, 409
BseDI CCNNGG 6 cut(s) 163, 164, 254, 407, 815, 1140
BseGI GGATG 4 cut(s) 361, 799, 868, 1132
BseJI GATNNNNATC 1 cut(s) 1235
BseLI CCNNNNNNNGG 7 cut(s) 169, 411, 412, 413, 810, 859, 1334
BseMII CTCAG 5 cut(s) 248, 254, 330, 567, 773
BseNI ACTGG 3 cut(s) 357, 367, 1071
BseXI GCAGC 2 cut(s) 137, 441
Bsh1236I CGCG 1 cut(s) 1107
BshFI GGCC 4 cut(s) 814, 967, 1172, 1327
BsiHKCI CYCGRG 2 cut(s) 183, 816
BsiSI CCGG 2 cut(s) 691, 782
BslI CCNNNNNNNGG 7 cut(s) 169, 411, 412, 413, 810, 859, 1334
BsmAI GTCTC 4 cut(s) 99, 243, 952, 1292
BsmI GAATGC 2 cut(s) 683, 1116
BsnI GGCC 4 cut(s) 814, 967, 1172, 1327
BsoBI CYCGRG 2 cut(s) 183, 816
Bsp1286I GDGCHC 1 cut(s) 207
Bsp1407I TGTACA 1 cut(s) 1206
Bsp143I GATC 8 cut(s) 393, 611, 698, 721, 754, 1165, 1230, 1255
Bsp68I TCGCGA 1 cut(s) 1107
BspACI CCGC 3 cut(s) 123, 207, 1199
BspANI GGCC 4 cut(s) 814, 967, 1172, 1327
BspCNI CTCAG 5 cut(s) 247, 253, 329, 568, 772
BspFNI CGCG 1 cut(s) 1107
BspLI GGNNCC 4 cut(s) 418, 714, 1071, 1232
BspMI ACCTGC 1 cut(s) 1192
BspPI GGATC 5 cut(s) 619, 749, 1225, 1238, 1250
BspQI GCTCTTC 1 cut(s) 14
BsrGI TGTACA 1 cut(s) 1206
BsrI ACTGG 3 cut(s) 357, 367, 1071
BssECI CCNNGG 6 cut(s) 163, 164, 254, 407, 815, 1140
BssMI GATC 8 cut(s) 393, 611, 698, 721, 754, 1165, 1230, 1255
BssT1I CCWWGG 2 cut(s) 254, 1140
Bst2UI CCWGG 2 cut(s) 165, 409
Bst4CI ACNGT 3 cut(s) 949, 1153, 1196
Bst6I CTCTTC 1 cut(s) 14
BstAUI TGTACA 1 cut(s) 1206
BstC8I GCNNGC 5 cut(s) 207, 298, 502, 506, 1030
BstDEI CTNAG 8 cut(s) 234, 240, 316, 576, 601, 759, 827, 1294
BstF5I GGATG 4 cut(s) 361, 799, 868, 1132
BstFNI CGCG 1 cut(s) 1107
BstKTI GATC 8 cut(s) 396, 614, 701, 724, 757, 1168, 1233, 1258
BstMAI GTCTC 4 cut(s) 99, 243, 952, 1292
BstMBI GATC 8 cut(s) 393, 611, 698, 721, 754, 1165, 1230, 1255
BstMWI GCNNNNNNNGC 3 cut(s) 30, 1113, 1178
BstNI CCWGG 2 cut(s) 165, 409
BstNSI RCATGY 2 cut(s) 310, 937
BstPAI GACNNNNGTC 2 cut(s) 848, 1194
BstSCI CCNGG 2 cut(s) 163, 407
BstUI CGCG 1 cut(s) 1107
BstV1I GCAGC 2 cut(s) 137, 441
BstV2I GAAGAC 1 cut(s) 753
BstX2I RGATCY 1 cut(s) 1230
BstYI RGATCY 1 cut(s) 1230
BsuI GTATCC 3 cut(s) 166, 811, 814
BsuRI GGCC 4 cut(s) 814, 967, 1172, 1327
BtsCI GGATG 4 cut(s) 361, 799, 868, 1132
BtsIMutI CAGTG 1 cut(s) 540
BtuMI TCGCGA 1 cut(s) 1107
BveI ACCTGC 1 cut(s) 1192
Cac8I GCNNGC 5 cut(s) 207, 298, 502, 506, 1030
CaiI CAGNNNCTG 1 cut(s) 644
Cfr13I GGNCC 2 cut(s) 874, 1069
Csp6I GTAC 6 cut(s) 69, 583, 590, 703, 1207, 1249
CviAII CATG 6 cut(s) 307, 586, 668, 934, 1010, 1113
CviQI GTAC 6 cut(s) 69, 583, 590, 703, 1207, 1249
DdeI CTNAG 8 cut(s) 234, 240, 316, 576, 601, 759, 827, 1294
DpnI GATC 8 cut(s) 395, 613, 700, 723, 756, 1167, 1232, 1257
DpnII GATC 8 cut(s) 393, 611, 698, 721, 754, 1165, 1230, 1255
EaeI YGGCCR 1 cut(s) 1170
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Eco130I CCWWGG 2 cut(s) 254, 1140
Eco24I GRGCYC 1 cut(s) 207
Eco47I GGWCC 2 cut(s) 874, 1069
Eco57I CTGAAG 3 cut(s) 756, 765, 901
Eco88I CYCGRG 2 cut(s) 183, 816
EcoRII CCWGG 2 cut(s) 163, 407
EcoT14I CCWWGG 2 cut(s) 254, 1140
EcoT22I ATGCAT 1 cut(s) 1118
EcoT38I GRGCYC 1 cut(s) 207
ErhI CCWWGG 2 cut(s) 254, 1140
FaeI CATG 6 cut(s) 310, 589, 671, 937, 1013, 1116
FalI AAGNNNNNCTT 4 cut(s) 64, 96, 428, 460
FatI CATG 6 cut(s) 306, 585, 667, 933, 1009, 1112
FauI CCCGC 1 cut(s) 214
Fnu4HI GCNGC 2 cut(s) 126, 430
FokI GGATG 4 cut(s) 368, 806, 875, 1139
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 2 cut(s) 126, 430
FspBI CTAG 1 cut(s) 1058
GluI GCNGC 2 cut(s) 126, 430
HaeIII GGCC 4 cut(s) 814, 967, 1172, 1327
HapII CCGG 2 cut(s) 691, 782
Hin1II CATG 6 cut(s) 310, 589, 671, 937, 1013, 1116
HinfI GANTC 5 cut(s) 731, 883, 890, 921, 1109
HpaII CCGG 2 cut(s) 691, 782
HphI GGTGA 1 cut(s) 324
Hpy166II GTNNAC 2 cut(s) 540, 1207
Hpy188I TCNGA 8 cut(s) 319, 342, 472, 487, 640, 736, 793, 920
Hpy188III TCNNGA 9 cut(s) 102, 185, 575, 659, 818, 887, 1058, 1106, 1163
Hpy8I GTNNAC 2 cut(s) 540, 1207
Hpy99I CGWCG 2 cut(s) 149, 425
HpyAV CCTTC 2 cut(s) 925, 1300
HpyCH4III ACNGT 3 cut(s) 949, 1153, 1196
HpyCH4IV ACGT 3 cut(s) 458, 465, 489
HpyCH4V TGCA 7 cut(s) 251, 300, 462, 905, 937, 1116, 1216
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 1113, 1178
HpyF3I CTNAG 8 cut(s) 234, 240, 316, 576, 601, 759, 827, 1294
HpySE526I ACGT 3 cut(s) 458, 465, 489
Hsp92II CATG 6 cut(s) 310, 589, 671, 937, 1013, 1116
Kzo9I GATC 8 cut(s) 393, 611, 698, 721, 754, 1165, 1230, 1255
LguI GCTCTTC 1 cut(s) 14
LmnI GCTCC 1 cut(s) 416
Lsp1109I GCAGC 2 cut(s) 137, 441
LweI GCATC 2 cut(s) 523, 1047
MaeI CTAG 1 cut(s) 1058
MaeII ACGT 3 cut(s) 458, 465, 489
MaeIII GTNAC 2 cut(s) 841, 1349
MalI GATC 8 cut(s) 395, 613, 700, 723, 756, 1167, 1232, 1257
MboI GATC 8 cut(s) 393, 611, 698, 721, 754, 1165, 1230, 1255
MboII GAAGA 5 cut(s) 31, 236, 617, 758, 1012
MflI RGATCY 1 cut(s) 1230
MhlI GDGCHC 1 cut(s) 207
MlsI TGGCCA 1 cut(s) 1172
MluCI AATT 6 cut(s) 76, 385, 785, 805, 1131, 1185
MluNI TGGCCA 1 cut(s) 1172
MlyI GAGTC 2 cut(s) 877, 930
MmeI TCCRAC 2 cut(s) 150, 1283
MnlI CCTC 5 cut(s) 570, 768, 825, 984, 1057
Mox20I TGGCCA 1 cut(s) 1172
Mph1103I ATGCAT 1 cut(s) 1118
MscI TGGCCA 1 cut(s) 1172
MseI TTAA 3 cut(s) 633, 654, 1148
MslI CAYNNNNRTG 1 cut(s) 147
Msp20I TGGCCA 1 cut(s) 1172
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 2 cut(s) 691, 782
MspR9I CCNGG 2 cut(s) 165, 409
Mva1269I GAATGC 2 cut(s) 683, 1116
MvaI CCWGG 2 cut(s) 165, 409
MvnI CGCG 1 cut(s) 1107
MwoI GCNNNNNNNGC 3 cut(s) 30, 1113, 1178
NdeII GATC 8 cut(s) 393, 611, 698, 721, 754, 1165, 1230, 1255
NlaIII CATG 6 cut(s) 310, 589, 671, 937, 1013, 1116
NlaIV GGNNCC 4 cut(s) 418, 714, 1071, 1232
NmuCI GTSAC 1 cut(s) 841
NruI TCGCGA 1 cut(s) 1107
NsiI ATGCAT 1 cut(s) 1118
NspI RCATGY 2 cut(s) 310, 937
PaqCI CACCTGC 1 cut(s) 1192
PasI CCCWGGG 1 cut(s) 164
PciI ACATGT 1 cut(s) 306
PciSI GCTCTTC 1 cut(s) 14
PctI GAATGC 2 cut(s) 683, 1116
PfeI GAWTC 3 cut(s) 731, 890, 1109
PflMI CCANNNNNTGG 1 cut(s) 810
PkrI GCNGC 2 cut(s) 127, 431
PleI GAGTC 2 cut(s) 877, 929
PpsI GAGTC 2 cut(s) 877, 929
PscI ACATGT 1 cut(s) 306
PshAI GACNNNNGTC 2 cut(s) 848, 1194
PshBI ATTAAT 1 cut(s) 654
PsiI TTATAA 1 cut(s) 770
Psp1406I AACGTT 1 cut(s) 465
Psp6I CCWGG 2 cut(s) 163, 407
PspGI CCWGG 2 cut(s) 163, 407
PspN4I GGNNCC 4 cut(s) 418, 714, 1071, 1232
PspPI GGNCC 2 cut(s) 874, 1069
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 1 cut(s) 1230
RruI TCGCGA 1 cut(s) 1107
RsaI GTAC 6 cut(s) 70, 584, 591, 704, 1208, 1250
RsaNI GTAC 6 cut(s) 69, 583, 590, 703, 1207, 1249
RseI CAYNNNNRTG 1 cut(s) 147
SapI GCTCTTC 1 cut(s) 14
SaqAI TTAA 3 cut(s) 633, 654, 1148
SatI GCNGC 2 cut(s) 126, 430
Sau3AI GATC 8 cut(s) 393, 611, 698, 721, 754, 1165, 1230, 1255
Sau96I GGNCC 2 cut(s) 874, 1069
SchI GAGTC 2 cut(s) 877, 930
ScrFI CCNGG 2 cut(s) 165, 409
SduI GDGCHC 1 cut(s) 207
SfaNI GCATC 2 cut(s) 523, 1047
SinI GGWCC 2 cut(s) 874, 1069
SmiMI CAYNNNNRTG 1 cut(s) 147
Sse9I AATT 6 cut(s) 76, 385, 785, 805, 1131, 1185
SsiI CCGC 3 cut(s) 123, 207, 1199
SspI AATATT 1 cut(s) 327
SspMI CTAG 1 cut(s) 1058
StyD4I CCNGG 2 cut(s) 163, 407
StyI CCWWGG 2 cut(s) 254, 1140
TaaI ACNGT 3 cut(s) 949, 1153, 1196
TaiI ACGT 3 cut(s) 461, 468, 492
TaqI TCGA 5 cut(s) 261, 279, 367, 660, 881
TasI AATT 6 cut(s) 76, 385, 785, 805, 1131, 1185
TatI WGTACW 3 cut(s) 68, 582, 1206
TfiI GAWTC 3 cut(s) 731, 890, 1109
Tru1I TTAA 3 cut(s) 633, 654, 1148
Tru9I TTAA 3 cut(s) 633, 654, 1148
TscAI CASTG 1 cut(s) 547
TseFI GTSAC 1 cut(s) 841
TseI GCWGC 2 cut(s) 125, 429
Tsp45I GTSAC 1 cut(s) 841
TspDTI ATGAA 6 cut(s) 32, 363, 879, 998, 1075, 1101
TspRI CASTG 1 cut(s) 547
Van91I CCANNNNNTGG 1 cut(s) 810
VpaK11BI GGWCC 2 cut(s) 874, 1069
VspI ATTAAT 1 cut(s) 654
XapI RAATTY 3 cut(s) 76, 385, 1131
XbaI TCTAGA 1 cut(s) 1057
XceI RCATGY 2 cut(s) 310, 937
XspI CTAG 1 cut(s) 1058
Zsp2I ATGCAT 1 cut(s) 1118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.