Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006277

Physical Location & Seq

Genomic Coordinates Reverse (-)

42292938 .. 42300850

Gene Structure 7913 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1291500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1413 bp

ATGCCATCTTTCAAGATGAAGGCTCAATCAAGCATGGGATGTTTACGAGAAAAAAATGGTCTCCGTGTATGTCAAAGGTCAAGTGTGATTTCCAAGAATTCTCATGCTAGGAAATCAGAACATTCAGAAGATTTTGACACTTGTAATAATTGTCAGGAGGTTTCTTCAAGCACACAAGTTTCTACTATAGAAATTGGAAGTGATGAGGCAATTGAACATCAGGAACTTCTGGGTGAACAAACTGGTCAAATTCAGAAGCAGTCATGTGATTCTCCCACCAAAAGAATGGACTCCTGTCAGGCCTGCCCATCAGACATAAAGACAATTTTCTCTCCTGCTTTGGAGTCGAGTGAAGTGAACGGCGAACAAAATAGTGAACCACATGCTGACATTGATGCAGTGTTAGGAGCTGGTGACAGTGATGATAACGGACGTTCATGTGATAATCGAACTTGTGATGTATCAGATTTCTACATTTCTGACATGATAATATCAAGCTTACCCTTCGACGAGAATGCATTTCATGATGATATAAGTGAAATGAATTGCTTCCAAGACTACAAATCAGCTGAACCAAATATGTTTTTTGATGTGTCTGAGCAATACATGATGTTGCCTTTCCTAGAAGACACAATCAGAAGCAGTAATTTTAATACTAATGATGACAAATCATGTGAAGAAGCCATGATAGATACAAATTGTGCTGAAATGTATTTGGGAATTGGTCAGATGAGCCCCTGCAACCTGGAAACGGTTGTTAACTCTTCTGACTCAGATCAGGCAGAGTCCTTTGATCCACAGTTGTTCATAAAAAACTTACCAGAATTATCAGAGGTGGTATCAAATTATCAACCCAACATATTGCCCGAGGAAGCTTCAAAAAGAAAGTCTGTAACTCTTGTACTTGATTTGGATGAAACACTGGTTCATTCTACATTGGAACATCGAGATGACGCAGACTTCACATTTACTGTCTTTTTCAACATGAAAGAGCACACGGTATATGTGAAGCGGAGGCCTCACCTGCAAACTTTCTTGGAGAGAGTTGCAGAAATGTTTGACGTTGTTATTTTTACTGCCAGCCAAAGCATATATGCAGCGCAGCTTCTTGATATATTGGATCCATGTGCAAAGTTTATATCTCGCCGAGTTTATCGTGAATCATGCATTTTCTTGGATGGCAGTTACACTAAAGATTTGACAGTTTTGGGTGTTGATCTTGCGAAAGTTGCCATAATTGATAATACTCCACAGGTCTTCCGCTTGCAAGTTAATAATGGAATTCCTATAAAGAGTTGGTTTGATGATCCGTCTGATAGTGCTTTGATTTCGTTACTTCCCTTCTTAGAGACCCTGGTTAATGCTGATGATGTTCGTCCTATAATTGCCAAAAAATTCGGTTACAAGGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0036211 GO:0042578 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NIF

Protein Analysis

471

Amino Acids

52.8

Weight (kDa)

4.46

Isoelectric Point (pI)

55.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	299 - 459	4e-56	NLI interacting factor-like phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006277)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18140 AT4G18140 AT4G18140 AT4G18140 AT4G18140 AT4G18140 AT5G46410 AT5G46410 AT5G46410
fragaria_vesca	FvH4_3g06370
malus_domestica	MD05G1291500.v1.1 MD10G1267500.v1.1
prunus_persica	Prupe.4G074000_v2.0.a1 Prupe.4G074000_v2.0.a1 Prupe.4G074000_v2.0.a1
pyrus_communis	pycom05g26800 pycom10g22350
rosa_chinensis	RchiOBHm_Chr5g0012931
rosa_laevigata	RLG00000031970
rosa_multiflora	Rmu_sc0006885.1_g000005
rosa_roxburghii	Rroxscaffold_1G00063650
rosa_rugosa	Rorug05G0005400
rosa_samantha	Rh5AG099600 Rh5BG096300 Rh5CG108500 Rh5DG094900
rosa_wichuraiana	Rw5G008700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1032
Acc36I	ACCTGC	1 cut(s)	1032
AciI	CCGC	2 cut(s)	1012, 1261
AclWI	GGATC	4 cut(s)	788, 1115, 1128, 1301
AcsI	RAATTY	4 cut(s)	97, 249, 1281, 1394
AfaI	GTAC	1 cut(s)	903
AfiI	CCNNNNNNNGG	1 cut(s)	751
AgsI	TTSAA	5 cut(s)	13, 168, 215, 879, 982
AjnI	CCWGG	2 cut(s)	744, 1353
AluBI	AGCT	5 cut(s)	410, 498, 569, 875, 1105
AluI	AGCT	5 cut(s)	410, 498, 569, 875, 1105
Alw21I	GWGCWC	1 cut(s)	996
Alw26I	GTCTC	2 cut(s)	65, 1343
AlwI	GGATC	4 cut(s)	788, 1115, 1128, 1301
Ama87I	CYCGRG	1 cut(s)	866
AoxI	GGCC	2 cut(s)	300, 1016
ApeKI	GCWGC	2 cut(s)	1097, 1102
ApoI	RAATTY	4 cut(s)	97, 249, 1281, 1394
ArsI	GACNNNNNNTTYG	2 cut(s)	872, 904
Asp700I	GAANNNNTTC	1 cut(s)	548
AspLEI	GCGC	1 cut(s)	1102
AsuHPI	GGTGA	3 cut(s)	245, 425, 1013
AvaI	CYCGRG	1 cut(s)	866
BamHI	GGATCC	1 cut(s)	1120
BanII	GRGCYC	1 cut(s)	737
BbsI	GAAGAC	2 cut(s)	633, 1249
Bbv12I	GWGCWC	1 cut(s)	996
BbvI	GCAGC	2 cut(s)	1109, 1114
BccI	CCATC	3 cut(s)	13, 316, 1172
BceAI	ACGGC	1 cut(s)	376
BcgI	CGANNNNNNTGC	2 cut(s)	497, 531
BciT130I	CCWGG	2 cut(s)	746, 1355
BcoDI	GTCTC	2 cut(s)	65, 1343
BfaI	CTAG	2 cut(s)	108, 623
BfmI	CTRYAG	1 cut(s)	186
BfuAI	ACCTGC	1 cut(s)	1032
BisI	GCNGC	2 cut(s)	1098, 1103
BlsI	GCNGC	2 cut(s)	1099, 1104
Bme1390I	CCNGG	2 cut(s)	746, 1355
BmeT110I	CYCGRG	1 cut(s)	866
BmiI	GGNNCC	1 cut(s)	1122
BmrFI	CCNGG	2 cut(s)	746, 1355
BmsI	GCATC	1 cut(s)	385
BoxI	GACNNNNGTC	1 cut(s)	294
BpiI	GAAGAC	2 cut(s)	633, 1249
BsaI	GGTCTC	2 cut(s)	65, 1343
BsaJI	CCNNGG	2 cut(s)	867, 1353
Bsc4I	CCNNNNNNNGG	1 cut(s)	751
Bse1I	ACTGG	2 cut(s)	247, 927
BseBI	CCWGG	2 cut(s)	746, 1355
BseDI	CCNNGG	2 cut(s)	867, 1353
BseGI	GGATG	3 cut(s)	44, 919, 1183
BseLI	CCNNNNNNNGG	1 cut(s)	751
BseMII	CTCAG	2 cut(s)	588, 786
BseNI	ACTGG	2 cut(s)	247, 927
BseXI	GCAGC	2 cut(s)	1109, 1114
BshFI	GGCC	2 cut(s)	302, 1018
BsiHKAI	GWGCWC	1 cut(s)	996
BsiHKCI	CYCGRG	1 cut(s)	866
BslI	CCNNNNNNNGG	1 cut(s)	751
BsmAI	GTCTC	2 cut(s)	65, 1343
BsmI	GAATGC	1 cut(s)	520
BsnI	GGCC	2 cut(s)	302, 1018
Bso31I	GGTCTC	2 cut(s)	65, 1343
BsoBI	CYCGRG	1 cut(s)	866
Bsp1286I	GDGCHC	2 cut(s)	737, 996
Bsp143I	GATC	5 cut(s)	775, 793, 1120, 1216, 1306
BspACI	CCGC	2 cut(s)	1012, 1261
BspANI	GGCC	2 cut(s)	302, 1018
BspCNI	CTCAG	2 cut(s)	589, 785
BspHI	TCATGA	1 cut(s)	523
BspLI	GGNNCC	1 cut(s)	1122
BspMI	ACCTGC	1 cut(s)	1032
BspPI	GGATC	4 cut(s)	788, 1115, 1128, 1301
BspTNI	GGTCTC	2 cut(s)	65, 1343
BsrI	ACTGG	2 cut(s)	247, 927
BssECI	CCNNGG	2 cut(s)	867, 1353
BssMI	GATC	5 cut(s)	775, 793, 1120, 1216, 1306
Bst2UI	CCWGG	2 cut(s)	746, 1355
Bst4CI	ACNGT	6 cut(s)	419, 754, 801, 973, 1000, 1204
Bst6I	CTCTTC	1 cut(s)	769
BstC8I	GCNNGC	3 cut(s)	304, 1081, 1265
BstDEI	CTNAG	3 cut(s)	597, 772, 1345
BstF5I	GGATG	3 cut(s)	44, 919, 1183
BstHHI	GCGC	1 cut(s)	1102
BstKTI	GATC	5 cut(s)	778, 796, 1123, 1219, 1309
BstMAI	GTCTC	2 cut(s)	65, 1343
BstMBI	GATC	5 cut(s)	775, 793, 1120, 1216, 1306
BstMWI	GCNNNNNNNGC	2 cut(s)	1024, 1229
BstNI	CCWGG	2 cut(s)	746, 1355
BstNSI	RCATGY	1 cut(s)	386
BstPAI	GACNNNNGTC	1 cut(s)	294
BstSCI	CCNGG	2 cut(s)	744, 1353
BstSFI	CTRYAG	1 cut(s)	186
BstV1I	GCAGC	2 cut(s)	1109, 1114
BstV2I	GAAGAC	2 cut(s)	633, 1249
BstX2I	RGATCY	1 cut(s)	1120
BstXI	CCANNNNNNTGG	1 cut(s)	286
BstYI	RGATCY	1 cut(s)	1120
BsuRI	GGCC	2 cut(s)	302, 1018
BtsCI	GGATG	3 cut(s)	44, 919, 1183
BtsI	GCAGTG	1 cut(s)	405
BtsIMutI	CAGTG	3 cut(s)	405, 424, 920
BveI	ACCTGC	1 cut(s)	1032
Cac8I	GCNNGC	3 cut(s)	304, 1081, 1265
CciI	TCATGA	1 cut(s)	523
CfoI	GCGC	1 cut(s)	1102
CseI	GACGC	1 cut(s)	962
Csp6I	GTAC	1 cut(s)	902
CviQI	GTAC	1 cut(s)	902
DdeI	CTNAG	3 cut(s)	597, 772, 1345
DpnI	GATC	5 cut(s)	777, 795, 1122, 1218, 1308
DpnII	GATC	5 cut(s)	775, 793, 1120, 1216, 1306
Eam1104I	CTCTTC	1 cut(s)	769
EarI	CTCTTC	1 cut(s)	769
Eco147I	AGGCCT	2 cut(s)	302, 1018
Eco24I	GRGCYC	1 cut(s)	737
Eco31I	GGTCTC	2 cut(s)	65, 1343
Eco88I	CYCGRG	1 cut(s)	866
EcoRI	GAATTC	2 cut(s)	97, 1281
EcoRII	CCWGG	2 cut(s)	744, 1353
EcoT22I	ATGCAT	2 cut(s)	520, 1169
EcoT38I	GRGCYC	1 cut(s)	737
Fnu4HI	GCNGC	2 cut(s)	1098, 1103
FokI	GGATG	3 cut(s)	51, 926, 1190
FriOI	GRGCYC	1 cut(s)	737
Fsp4HI	GCNGC	2 cut(s)	1098, 1103
FspBI	CTAG	2 cut(s)	108, 623
GlaI	GCGC	1 cut(s)	1101
GluI	GCNGC	2 cut(s)	1098, 1103
HaeIII	GGCC	2 cut(s)	302, 1018
HgaI	GACGC	1 cut(s)	962
HhaI	GCGC	1 cut(s)	1102
Hin6I	GCGC	1 cut(s)	1100
HinP1I	GCGC	1 cut(s)	1100
HincII	GTYRAC	1 cut(s)	760
HindII	GTYRAC	1 cut(s)	760
HindIII	AAGCTT	2 cut(s)	496, 873
HinfI	GANTC	6 cut(s)	269, 290, 344, 770, 785, 1160
HpaI	GTTAAC	1 cut(s)	760
HphI	GGTGA	3 cut(s)	245, 425, 1013
Hpy166II	GTNNAC	5 cut(s)	44, 236, 358, 377, 760
Hpy188III	TCNNGA	7 cut(s)	13, 155, 221, 524, 947, 1109, 1157
Hpy8I	GTNNAC	5 cut(s)	44, 236, 358, 377, 760
Hpy99I	CGWCG	1 cut(s)	512
HpyAV	CCTTC	3 cut(s)	13, 514, 1351
HpyCH4III	ACNGT	6 cut(s)	419, 754, 801, 973, 1000, 1204
HpyCH4IV	ACGT	2 cut(s)	433, 1062
HpyCH4V	TGCA	9 cut(s)	398, 518, 741, 1027, 1049, 1097, 1130, 1167, 1267
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1024, 1229
HpyF3I	CTNAG	3 cut(s)	597, 772, 1345
HpySE526I	ACGT	2 cut(s)	433, 1062
HspAI	GCGC	1 cut(s)	1100
KspAI	GTTAAC	1 cut(s)	760
Kzo9I	GATC	5 cut(s)	775, 793, 1120, 1216, 1306
LmnI	GCTCC	1 cut(s)	407
Lsp1109I	GCAGC	2 cut(s)	1109, 1114
LweI	GCATC	1 cut(s)	385
MaeI	CTAG	2 cut(s)	108, 623
MaeII	ACGT	2 cut(s)	433, 1062
MaeIII	GTNAC	5 cut(s)	413, 892, 1184, 1332, 1400
MalI	GATC	5 cut(s)	777, 795, 1122, 1218, 1308
MboI	GATC	5 cut(s)	775, 793, 1120, 1216, 1306
MboII	GAAGA	6 cut(s)	140, 156, 638, 689, 756, 1249
MfeI	CAATTG	1 cut(s)	210
MflI	RGATCY	1 cut(s)	1120
MhlI	GDGCHC	2 cut(s)	737, 996
MlyI	GAGTC	4 cut(s)	284, 353, 764, 794
MnlI	CCTC	6 cut(s)	151, 199, 826, 862, 1008, 1029
Mph1103I	ATGCAT	2 cut(s)	520, 1169
MroXI	GAANNNNTTC	1 cut(s)	548
MseI	TTAA	4 cut(s)	651, 759, 1272, 1359
MslI	CAYNNNNRTG	1 cut(s)	948
MspA1I	CMGCKG	1 cut(s)	569
MspR9I	CCNGG	2 cut(s)	746, 1355
MunI	CAATTG	1 cut(s)	210
Mva1269I	GAATGC	1 cut(s)	520
MvaI	CCWGG	2 cut(s)	746, 1355
MwoI	GCNNNNNNNGC	2 cut(s)	1024, 1229
NdeII	GATC	5 cut(s)	775, 793, 1120, 1216, 1306
NlaIV	GGNNCC	1 cut(s)	1122
NmeAIII	GCCGAG	1 cut(s)	1172
NmuCI	GTSAC	1 cut(s)	413
NsiI	ATGCAT	2 cut(s)	520, 1169
NspI	RCATGY	1 cut(s)	386
PagI	TCATGA	1 cut(s)	523
PaqCI	CACCTGC	1 cut(s)	1032
PceI	AGGCCT	2 cut(s)	302, 1018
PctI	GAATGC	1 cut(s)	520
PdmI	GAANNNNTTC	1 cut(s)	548
PfeI	GAWTC	2 cut(s)	269, 1160
PkrI	GCNGC	2 cut(s)	1099, 1104
PleI	GAGTC	4 cut(s)	284, 352, 764, 793
PpsI	GAGTC	4 cut(s)	284, 352, 764, 793
PshAI	GACNNNNGTC	1 cut(s)	294
Psp6I	CCWGG	2 cut(s)	744, 1353
PspGI	CCWGG	2 cut(s)	744, 1353
PspN4I	GGNNCC	1 cut(s)	1122
PsuI	RGATCY	1 cut(s)	1120
PvuII	CAGCTG	1 cut(s)	569
RsaI	GTAC	1 cut(s)	903
RsaNI	GTAC	1 cut(s)	902
RseI	CAYNNNNRTG	1 cut(s)	948
SaqAI	TTAA	4 cut(s)	651, 759, 1272, 1359
SatI	GCNGC	2 cut(s)	1098, 1103
Sau3AI	GATC	5 cut(s)	775, 793, 1120, 1216, 1306
SchI	GAGTC	4 cut(s)	284, 353, 764, 794
ScrFI	CCNGG	2 cut(s)	746, 1355
SduI	GDGCHC	2 cut(s)	737, 996
SfaNI	GCATC	1 cut(s)	385
SfcI	CTRYAG	1 cut(s)	186
SmiMI	CAYNNNNRTG	1 cut(s)	948
SseBI	AGGCCT	2 cut(s)	302, 1018
SsiI	CCGC	2 cut(s)	1012, 1261
SspMI	CTAG	2 cut(s)	108, 623
StuI	AGGCCT	2 cut(s)	302, 1018
StyD4I	CCNGG	2 cut(s)	744, 1353
TaaI	ACNGT	6 cut(s)	419, 754, 801, 973, 1000, 1204
TaiI	ACGT	2 cut(s)	436, 1065
TaqI	TCGA	4 cut(s)	347, 448, 507, 946
TatI	WGTACW	1 cut(s)	901
TfiI	GAWTC	2 cut(s)	269, 1160
Tru1I	TTAA	4 cut(s)	651, 759, 1272, 1359
Tru9I	TTAA	4 cut(s)	651, 759, 1272, 1359
TscAI	CASTG	3 cut(s)	405, 424, 927
TseFI	GTSAC	1 cut(s)	413
TseI	GCWGC	2 cut(s)	1097, 1102
Tsp45I	GTSAC	1 cut(s)	413
TspDTI	ATGAA	8 cut(s)	32, 426, 512, 557, 796, 917, 930, 1001
TspGWI	ACGGA	3 cut(s)	53, 444, 1299
TspRI	CASTG	3 cut(s)	405, 424, 927
XapI	RAATTY	4 cut(s)	97, 249, 1281, 1394
XceI	RCATGY	1 cut(s)	386
XcmI	CCANNNNNNNNNTGG	1 cut(s)	283
XmnI	GAANNNNTTC	1 cut(s)	548
XspI	CTAG	2 cut(s)	108, 623
Zsp2I	ATGCAT	2 cut(s)	520, 1169

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.