Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006277

Physical Location & Seq

Genomic Coordinates Reverse (-)

36041477 .. 36048510

Gene Structure 7034 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1267500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGCCATCTTTCAAAATGAAGGCTCAATCAAGCACAGGGTGTTTACGAGAAAAAAATTGTCTTCGTGTATGTCAAAGGTCAAGTGTGATTTCCAAGAAAGCATGTGTTCATGCTAGGAAATCTGAACATACAGAAGATTTTGACACTTGTAAAAATTGTCAGGAGGTTTCTTCAAGCACACAAGTTTCTACTCTAGAAATTGGAAGTGATGAAGCAACTGAACATCAGGAATTTCTGGGTGAACAAATTGGTCAAATTCAGCAGCAGTCATGTGATTCTTCCACCAAAAGAATGGACTCCTTTCACGCCTGCCCATCAAACTTAGAGACGATTTTCTCTCCTGCTTTGGAGTCCGGTGAAGTCAACGGCAAACAAAATAGTGAACCAAATGCTGACATTGATGCAGGGTTAGGAACTGGTGACAGTGATGATAACGGAAGTTCATGTAATAATCGAAATTGCGATGTATCAGATTTCTATATTTCTGATATGATCATTTCAAGCTTACCCTTCGATGAGAATGCATTTCATGATGATATTAGTGAAATGAATTACTTTCCAGACTACAAATATGCTGAAACAAATATGTTTTTTGATGTGTCTGAGCAATACATGGTATTGCCTTTCCTAGAAGACACAATCAGAAGCAGTAATTTTTATACTAATGATGACAAATCATGTGAAGAAGCCATGGTAGATACAAATTGTGCTGGAATGTATTTGGGAATTGGTCAGATAAGCCCCTGCAACCAGGAAACGGTTGTCAACTCTTCCGACTCAGATCAGGCAGAGTCCTTTGATCCACAGTTGTTTATAAAAAACTTACCAGAATTATCAGATGTGGTATCAAACTATCAACCCAACATTTTGCCTGAGGAAGCTTCAAAAAGAAAGTCTGTAACACTTGTGCTTGATTTGGATGAAACACTGGTTCATTCTACATTGGACCATCGAGATGACGCAGACTTCACATTTACCGTCTTTTTCAACATGAAGGAGCACACGGTATATGTGAAACGGAGGCCTTACCTCCAAATTTTCTTGGAGAGAGTTGCAGAAATGTTTGAAGTCGTTATTTTTACTGCCAGCCAAAGCATATATGCAGCGCAACTTCTTGATGTATTGGATCCATATGCAAAGTTTATATCTCGCCGAGTTTATCGTGAATCGTGCATTTTCTCAGATGGCAGTTACACCAAAGATCTGACAGTTTTGGGTGTTGATCTTGCAAAAGTTGCCATAATTGATAATACTCCACAGGTTTTCCGCTTGCAAGTTAACAATGGAATTCCAATAAAGAGTTGGTTTGATGATCCGTCCGATAGTGCTTTGATTTCGTTACTTCCCTTCTTAGAGACCCTGGTTAATGCTGATGATGTTCGTCCTATAATTGCCAAGAGATTCGGTAACATGGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0036211 GO:0042578 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NIF

Protein Analysis

473

Amino Acids

53.06

Weight (kDa)

4.45

Isoelectric Point (pI)

52.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	301 - 461	2.4e-56	NLI interacting factor-like phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006277)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18140 AT4G18140 AT4G18140 AT4G18140 AT4G18140 AT4G18140 AT5G46410 AT5G46410 AT5G46410
fragaria_vesca	FvH4_3g06370
malus_domestica	MD05G1291500.v1.1 MD10G1267500.v1.1
prunus_persica	Prupe.4G074000_v2.0.a1 Prupe.4G074000_v2.0.a1 Prupe.4G074000_v2.0.a1
pyrus_communis	pycom05g26800 pycom10g22350
rosa_chinensis	RchiOBHm_Chr5g0012931
rosa_laevigata	RLG00000031970
rosa_multiflora	Rmu_sc0006885.1_g000005
rosa_roxburghii	Rroxscaffold_1G00063650
rosa_rugosa	Rorug05G0005400
rosa_samantha	Rh5AG099600 Rh5BG096300 Rh5CG108500 Rh5DG094900
rosa_wichuraiana	Rw5G008700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	815
AciI	CCGC	1 cut(s)	1267
AclWI	GGATC	4 cut(s)	794, 1121, 1134, 1307
AcsI	RAATTY	4 cut(s)	230, 255, 1035, 1287
AdeI	CACNNNGTG	1 cut(s)	39
AfiI	CCNNNNNNNGG	1 cut(s)	757
AgsI	TTSAA	6 cut(s)	13, 174, 501, 885, 988, 1067
AjnI	CCWGG	2 cut(s)	750, 1359
AluBI	AGCT	2 cut(s)	504, 881
AluI	AGCT	2 cut(s)	504, 881
Alw21I	GWGCWC	1 cut(s)	1002
Alw26I	GTCTC	2 cut(s)	320, 1349
AlwI	GGATC	4 cut(s)	794, 1121, 1134, 1307
AoxI	GGCC	1 cut(s)	1022
ApeKI	GCWGC	2 cut(s)	262, 1103
ApoI	RAATTY	4 cut(s)	230, 255, 1035, 1287
ArsI	GACNNNNNNTTYG	2 cut(s)	878, 910
AspLEI	GCGC	1 cut(s)	1108
AspS9I	GGNCC	1 cut(s)	946
AsuHPI	GGTGA	3 cut(s)	251, 368, 431
AvaII	GGWCC	1 cut(s)	946
AxyI	CCTNAGG	1 cut(s)	873
BamHI	GGATCC	1 cut(s)	1126
BbsI	GAAGAC	2 cut(s)	53, 639
Bbv12I	GWGCWC	1 cut(s)	1002
BbvI	GCAGC	2 cut(s)	274, 1115
BccI	CCATC	4 cut(s)	13, 322, 957, 1178
BceAI	ACGGC	1 cut(s)	382
BcgI	CGANNNNNNTGC	2 cut(s)	503, 537
BciT130I	CCWGG	2 cut(s)	752, 1361
BclI	TGATCA	1 cut(s)	492
BcoDI	GTCTC	2 cut(s)	320, 1349
BfaI	CTAG	3 cut(s)	114, 194, 629
BglII	AGATCT	1 cut(s)	1201
BisI	GCNGC	2 cut(s)	263, 1104
BlsI	GCNGC	2 cut(s)	264, 1105
Bme1390I	CCNGG	2 cut(s)	752, 1361
Bme18I	GGWCC	1 cut(s)	946
BmgT120I	GGNCC	1 cut(s)	946
BmiI	GGNNCC	1 cut(s)	1128
BmrFI	CCNGG	2 cut(s)	752, 1361
BmsI	GCATC	1 cut(s)	391
BpiI	GAAGAC	2 cut(s)	53, 639
BsaI	GGTCTC	1 cut(s)	1349
BsaJI	CCNNGG	2 cut(s)	690, 1359
BsaWI	WCCGGW	1 cut(s)	353
Bsc4I	CCNNNNNNNGG	1 cut(s)	757
Bse1I	ACTGG	2 cut(s)	421, 933
Bse21I	CCTNAGG	1 cut(s)	873
BseBI	CCWGG	2 cut(s)	752, 1361
BseDI	CCNNGG	2 cut(s)	690, 1359
BseGI	GGATG	1 cut(s)	925
BseLI	CCNNNNNNNGG	1 cut(s)	757
BseMII	CTCAG	4 cut(s)	594, 792, 864, 1194
BseNI	ACTGG	2 cut(s)	421, 933
BseXI	GCAGC	2 cut(s)	274, 1115
BshFI	GGCC	1 cut(s)	1024
BsiHKAI	GWGCWC	1 cut(s)	1002
BsiSI	CCGG	1 cut(s)	354
BslI	CCNNNNNNNGG	1 cut(s)	757
BsmAI	GTCTC	2 cut(s)	320, 1349
BsmBI	CGTCTC	1 cut(s)	320
BsmI	GAATGC	1 cut(s)	526
BsnI	GGCC	1 cut(s)	1024
Bso31I	GGTCTC	1 cut(s)	1349
Bsp1286I	GDGCHC	1 cut(s)	1002
Bsp143I	GATC	7 cut(s)	492, 781, 799, 1126, 1201, 1222, 1312
Bsp19I	CCATGG	1 cut(s)	690
BspACI	CCGC	1 cut(s)	1267
BspANI	GGCC	1 cut(s)	1024
BspCNI	CTCAG	4 cut(s)	595, 791, 865, 1193
BspHI	TCATGA	1 cut(s)	529
BspLI	GGNNCC	1 cut(s)	1128
BspPI	GGATC	4 cut(s)	794, 1121, 1134, 1307
BspTNI	GGTCTC	1 cut(s)	1349
BsrI	ACTGG	2 cut(s)	421, 933
BssECI	CCNNGG	2 cut(s)	690, 1359
BssMI	GATC	7 cut(s)	492, 781, 799, 1126, 1201, 1222, 1312
BssT1I	CCWWGG	1 cut(s)	690
Bst2UI	CCWGG	2 cut(s)	752, 1361
Bst4CI	ACNGT	6 cut(s)	425, 760, 807, 979, 1006, 1210
Bst6I	CTCTTC	1 cut(s)	775
BstAPI	GCANNNNNTGC	1 cut(s)	1235
BstC8I	GCNNGC	3 cut(s)	310, 1087, 1271
BstDEI	CTNAG	6 cut(s)	322, 603, 778, 873, 1180, 1351
BstDSI	CCRYGG	1 cut(s)	690
BstF5I	GGATG	1 cut(s)	925
BstHHI	GCGC	1 cut(s)	1108
BstKTI	GATC	7 cut(s)	495, 784, 802, 1129, 1204, 1225, 1315
BstMAI	GTCTC	2 cut(s)	320, 1349
BstMBI	GATC	7 cut(s)	492, 781, 799, 1126, 1201, 1222, 1312
BstMWI	GCNNNNNNNGC	1 cut(s)	1235
BstNI	CCWGG	2 cut(s)	752, 1361
BstNSI	RCATGY	1 cut(s)	105
BstSCI	CCNGG	2 cut(s)	750, 1359
BstV1I	GCAGC	2 cut(s)	274, 1115
BstV2I	GAAGAC	2 cut(s)	53, 639
BstX2I	RGATCY	2 cut(s)	1126, 1201
BstXI	CCANNNNNNTGG	1 cut(s)	292
BstYI	RGATCY	2 cut(s)	1126, 1201
Bsu36I	CCTNAGG	1 cut(s)	873
BsuRI	GGCC	1 cut(s)	1024
BtgI	CCRYGG	1 cut(s)	690
BtgZI	GCGATG	1 cut(s)	477
BtsCI	GGATG	1 cut(s)	925
BtsIMutI	CAGTG	2 cut(s)	430, 926
Cac8I	GCNNGC	3 cut(s)	310, 1087, 1271
CciI	TCATGA	1 cut(s)	529
CfoI	GCGC	1 cut(s)	1108
Cfr13I	GGNCC	1 cut(s)	946
CseI	GACGC	1 cut(s)	968
CviJI	RGCY	7 cut(s)	23, 504, 689, 741, 881, 1024, 1089
CviKI_1	RGCY	7 cut(s)	23, 504, 689, 741, 881, 1024, 1089
DdeI	CTNAG	6 cut(s)	322, 603, 778, 873, 1180, 1351
DpnI	GATC	7 cut(s)	494, 783, 801, 1128, 1203, 1224, 1314
DpnII	GATC	7 cut(s)	492, 781, 799, 1126, 1201, 1222, 1312
DraIII	CACNNNGTG	1 cut(s)	39
Eam1104I	CTCTTC	1 cut(s)	775
EarI	CTCTTC	1 cut(s)	775
Eco130I	CCWWGG	1 cut(s)	690
Eco147I	AGGCCT	1 cut(s)	1024
Eco31I	GGTCTC	1 cut(s)	1349
Eco47I	GGWCC	1 cut(s)	946
Eco81I	CCTNAGG	1 cut(s)	873
EcoRI	GAATTC	1 cut(s)	1287
EcoRII	CCWGG	2 cut(s)	750, 1359
EcoT14I	CCWWGG	1 cut(s)	690
EcoT22I	ATGCAT	1 cut(s)	526
ErhI	CCWWGG	1 cut(s)	690
Esp3I	CGTCTC	1 cut(s)	320
FauNDI	CATATG	1 cut(s)	1132
FbaI	TGATCA	1 cut(s)	492
Fnu4HI	GCNGC	2 cut(s)	263, 1104
FokI	GGATG	1 cut(s)	932
Fsp4HI	GCNGC	2 cut(s)	263, 1104
FspBI	CTAG	3 cut(s)	114, 194, 629
GlaI	GCGC	1 cut(s)	1107
GluI	GCNGC	2 cut(s)	263, 1104
HaeIII	GGCC	1 cut(s)	1024
HapII	CCGG	1 cut(s)	354
HgaI	GACGC	1 cut(s)	968
HhaI	GCGC	1 cut(s)	1108
Hin6I	GCGC	1 cut(s)	1106
HinP1I	GCGC	1 cut(s)	1106
HincII	GTYRAC	3 cut(s)	364, 766, 1279
HindII	GTYRAC	3 cut(s)	364, 766, 1279
HindIII	AAGCTT	2 cut(s)	502, 879
HinfI	GANTC	7 cut(s)	275, 296, 350, 776, 791, 1166, 1401
HpaI	GTTAAC	1 cut(s)	1279
HpaII	CCGG	1 cut(s)	354
HphI	GGTGA	3 cut(s)	251, 368, 431
Hpy166II	GTNNAC	6 cut(s)	44, 242, 364, 383, 766, 1279
Hpy188III	TCNNGA	8 cut(s)	161, 194, 227, 530, 560, 953, 1115, 1163
Hpy8I	GTNNAC	6 cut(s)	44, 242, 364, 383, 766, 1279
HpyAV	CCTTC	4 cut(s)	13, 520, 988, 1357
HpyCH4III	ACNGT	6 cut(s)	425, 760, 807, 979, 1006, 1210
HpyCH4V	TGCA	9 cut(s)	404, 524, 747, 1055, 1103, 1136, 1173, 1229, 1273
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1235
HpyF3I	CTNAG	6 cut(s)	322, 603, 778, 873, 1180, 1351
HspAI	GCGC	1 cut(s)	1106
Ksp22I	TGATCA	1 cut(s)	492
KspAI	GTTAAC	1 cut(s)	1279
Kzo9I	GATC	7 cut(s)	492, 781, 799, 1126, 1201, 1222, 1312
LmnI	GCTCC	1 cut(s)	997
Lsp1109I	GCAGC	2 cut(s)	274, 1115
LweI	GCATC	1 cut(s)	391
MaeI	CTAG	3 cut(s)	114, 194, 629
MaeIII	GTNAC	5 cut(s)	419, 898, 1190, 1338, 1406
MalI	GATC	7 cut(s)	494, 783, 801, 1128, 1203, 1224, 1314
MboI	GATC	7 cut(s)	492, 781, 799, 1126, 1201, 1222, 1312
MboII	GAAGA	7 cut(s)	53, 146, 162, 270, 644, 695, 762
MflI	RGATCY	2 cut(s)	1126, 1201
MhlI	GDGCHC	1 cut(s)	1002
MlyI	GAGTC	4 cut(s)	290, 359, 770, 800
MmeI	TCCRAC	1 cut(s)	798
MnlI	CCTC	4 cut(s)	157, 868, 1014, 1040
Mph1103I	ATGCAT	1 cut(s)	526
MseI	TTAA	2 cut(s)	1278, 1365
MslI	CAYNNNNRTG	1 cut(s)	954
MspI	CCGG	1 cut(s)	354
MspR9I	CCNGG	2 cut(s)	752, 1361
Mva1269I	GAATGC	1 cut(s)	526
MvaI	CCWGG	2 cut(s)	752, 1361
MwoI	GCNNNNNNNGC	1 cut(s)	1235
NcoI	CCATGG	1 cut(s)	690
NdeI	CATATG	1 cut(s)	1132
NdeII	GATC	7 cut(s)	492, 781, 799, 1126, 1201, 1222, 1312
NlaIV	GGNNCC	1 cut(s)	1128
NmeAIII	GCCGAG	1 cut(s)	1178
NmuCI	GTSAC	1 cut(s)	419
NsiI	ATGCAT	1 cut(s)	526
NspI	RCATGY	1 cut(s)	105
PagI	TCATGA	1 cut(s)	529
PceI	AGGCCT	1 cut(s)	1024
PctI	GAATGC	1 cut(s)	526
PfeI	GAWTC	3 cut(s)	275, 1166, 1401
PkrI	GCNGC	2 cut(s)	264, 1105
PleI	GAGTC	4 cut(s)	290, 358, 770, 799
PpsI	GAGTC	4 cut(s)	290, 358, 770, 799
PsiI	TTATAA	1 cut(s)	815
Psp6I	CCWGG	2 cut(s)	750, 1359
PspGI	CCWGG	2 cut(s)	750, 1359
PspN4I	GGNNCC	1 cut(s)	1128
PspPI	GGNCC	1 cut(s)	946
PsuI	RGATCY	2 cut(s)	1126, 1201
RseI	CAYNNNNRTG	1 cut(s)	954
SaqAI	TTAA	2 cut(s)	1278, 1365
SatI	GCNGC	2 cut(s)	263, 1104
Sau3AI	GATC	7 cut(s)	492, 781, 799, 1126, 1201, 1222, 1312
Sau96I	GGNCC	1 cut(s)	946
SchI	GAGTC	4 cut(s)	290, 359, 770, 800
ScrFI	CCNGG	2 cut(s)	752, 1361
SduI	GDGCHC	1 cut(s)	1002
SetI	ASST	6 cut(s)	80, 168, 506, 883, 1032, 1263
SfaNI	GCATC	1 cut(s)	391
SinI	GGWCC	1 cut(s)	946
SmiMI	CAYNNNNRTG	1 cut(s)	954
SseBI	AGGCCT	1 cut(s)	1024
SsiI	CCGC	1 cut(s)	1267
SspMI	CTAG	3 cut(s)	114, 194, 629
StuI	AGGCCT	1 cut(s)	1024
StyD4I	CCNGG	2 cut(s)	750, 1359
StyI	CCWWGG	1 cut(s)	690
TaaI	ACNGT	6 cut(s)	425, 760, 807, 979, 1006, 1210
TaqI	TCGA	3 cut(s)	454, 513, 952
TfiI	GAWTC	3 cut(s)	275, 1166, 1401
Tru1I	TTAA	2 cut(s)	1278, 1365
Tru9I	TTAA	2 cut(s)	1278, 1365
TscAI	CASTG	2 cut(s)	430, 933
TseFI	GTSAC	1 cut(s)	419
TseI	GCWGC	2 cut(s)	262, 1103
Tsp45I	GTSAC	1 cut(s)	419
TspDTI	ATGAA	9 cut(s)	32, 98, 225, 432, 518, 563, 923, 936, 1007
TspGWI	ACGGA	3 cut(s)	450, 1033, 1305
TspRI	CASTG	2 cut(s)	430, 933
VpaK11BI	GGWCC	1 cut(s)	946
XapI	RAATTY	4 cut(s)	230, 255, 1035, 1287
XbaI	TCTAGA	1 cut(s)	193
XceI	RCATGY	1 cut(s)	105
XcmI	CCANNNNNNNNNTGG	2 cut(s)	289, 1299
XspI	CTAG	3 cut(s)	114, 194, 629
Zsp2I	ATGCAT	1 cut(s)	526

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.