AT4G18530

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004588
Physical Location & Seq
Forward (+)
10217338 .. 10220550
3213 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G18530.2

Sequence Viewer

Length: 1170 bp
ATGAAACAGGGTTCAATGAATAGATCGTGTCTCTGTAGTGTCTTAATCACCACTGCTCTGATTTGTGGTGCTTACTTCATTTGCAATGCTTATCTTGCTAAAGACTTTAAAGAGAAGTTGCTGAAGTGGGAAATCACTGATAAGATGCATAACAGTACTGATAAGATGCAGAATGCAACAACAACCAGTACATGCAAGAATTTCAATAAGCCAGTGGGTACTGAAGCACTACCGCAAGGAATTATCGAGAAAACATCGAACCTGGAAACACAACATCTATGGAACTACGATGACACAAAAAAGAGAAGGCCTAACCATTCGATGAGTTTGTTAGCCATGGCGGTCGGTATCAAGCAAAAGGAGCTAGTTAACAAAGTTATCCAAAAGTTTCCTCCTCGAGATTTCGCGGTCATGCTTTTTCATTATGATGGTGTTGTCGATGACTGGAAGCAGTATCCATGGAATAATCATGCGATTCATGTTTCCGTGATGAATCAAACAAAATGGTGGTTCGCCAAGCGATTCTTGCATCCCGATATAGTTGCAGAGTACGAGTATATATTTCTTTGGGACGAAGATCTTGGTGTTGGTCATTTCAATCCTCAACGATATCTATCTATTGTCAAAGAAGAGGGGCTTGAGATATCGCAACCTGCTCTTGACACTTCAAAATCAGAAGTGCATCATCCTATAACCGCTCGTCGAAAAAAATCAAAAGTTCATAGAAGAATGTATAAATACAAAGGTAGCGGGCGATGTGATGACCATAGCACCAATCCTCCTTGCATCGGGTGGGTGGAAATGATGGCACCTGTTTTCTCTAGAGCTGCATGGAGATGTTCTTGGTATATGATTCAGAATGATTTGATCCATGCTTGGGGTCTGGATACGCAGCTTGGTTATTGTGCTCAAGGTGACCGAAAGAAAAATGTCGGTGTTGTTGATGCGGAGTACATAATTCATTATGGTCTTCCAACGCTCGGTGTGGTTGAAACCGCTTCAAGCGCTTTGCGGAATGAGACAGACTCGAAATCAACGGAATCATTAGAGTCTCGTGAAGTGGATAATAGACCAGAAGTGAGGATGAAATCATTTGTGGAGATGAAGAGATTCAAGGAACGTTGGAAGAAAGCTGTGAGGGATGATACATGTTGGGTTGATCCGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

389

Amino Acids

45.18

Weight (kDa)

8.82

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 70 - 304 2.1e-124 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 661
AccB1I GGYRCC 1 cut(s) 808
AccBSI CCGCTC 1 cut(s) 698
AccII CGCG 1 cut(s) 407
AciI CCGC 8 cut(s) 233, 341, 407, 696, 750, 947, 996, 1012
AclI AACGTT 1 cut(s) 1120
AclWI GGATC 2 cut(s) 862, 1154
AcsI RAATTY 1 cut(s) 199
AcuI CTGAAG 2 cut(s) 143, 243
AfaI GTAC 5 cut(s) 157, 190, 220, 551, 953
AfeI AGCGCT 1 cut(s) 1006
AfiI CCNNNNNNNGG 3 cut(s) 788, 877, 980
AflIII ACRYGT 1 cut(s) 1148
AgsI TTSAA 7 cut(s) 15, 205, 598, 669, 992, 1002, 1114
AjnI CCWGG 1 cut(s) 261
AluBI AGCT 4 cut(s) 364, 827, 895, 1133
AluI AGCT 4 cut(s) 364, 827, 895, 1133
Alw21I GWGCWC 1 cut(s) 910
Alw26I GTCTC 3 cut(s) 35, 1013, 1056
AlwI GGATC 2 cut(s) 862, 1154
Ama87I CYCGRG 1 cut(s) 396
Aor51HI AGCGCT 1 cut(s) 1006
AoxI GGCC 1 cut(s) 308
ApeKI GCWGC 2 cut(s) 827, 892
ApoI RAATTY 1 cut(s) 199
Asp700I GAANNNNTTC 1 cut(s) 1109
AspLEI GCGC 1 cut(s) 1007
AsuHPI GGTGA 2 cut(s) 40, 926
AvaI CYCGRG 1 cut(s) 396
BanI GGYRCC 1 cut(s) 808
BauI CACGAG 1 cut(s) 1053
BbsI GAAGAC 1 cut(s) 962
Bbv12I GWGCWC 1 cut(s) 910
BbvI GCAGC 2 cut(s) 814, 904
BccI CCATC 2 cut(s) 422, 799
BcgI CGANNNNNNTGC 2 cut(s) 524, 558
BciT130I CCWGG 1 cut(s) 263
BciVI GTATCC 2 cut(s) 465, 880
BcoDI GTCTC 3 cut(s) 35, 1013, 1056
BfaI CTAG 2 cut(s) 365, 822
BfmI CTRYAG 1 cut(s) 34
BfoI RGCGCY 1 cut(s) 1008
BfuAI ACCTGC 1 cut(s) 661
BfuI GTATCC 2 cut(s) 465, 880
BglII AGATCT 1 cut(s) 577
BisI GCNGC 2 cut(s) 828, 893
BlsI GCNGC 2 cut(s) 829, 894
BmcAI AGTACT 1 cut(s) 157
Bme1390I CCNGG 1 cut(s) 263
BmeT110I CYCGRG 1 cut(s) 396
BmiI GGNNCC 1 cut(s) 810
BmrFI CCNGG 1 cut(s) 263
BmsI GCATC 6 cut(s) 135, 156, 538, 691, 795, 934
BpiI GAAGAC 1 cut(s) 962
BplI GAGNNNNNCTC 2 cut(s) 1010, 1042
BpuEI CTTGAG 2 cut(s) 659, 894
BsaBI GATNNNNATC 1 cut(s) 613
BsaJI CCNNGG 2 cut(s) 336, 458
BsaXI ACNNNNNCTCC 2 cut(s) 763, 793
Bsc4I CCNNNNNNNGG 3 cut(s) 788, 877, 980
Bse1I ACTGG 3 cut(s) 186, 212, 449
Bse3DI GCAATG 1 cut(s) 91
Bse8I GATNNNNATC 1 cut(s) 613
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 2 cut(s) 336, 458
BseGI GGATG 4 cut(s) 529, 685, 1089, 1147
BseJI GATNNNNATC 1 cut(s) 613
BseLI CCNNNNNNNGG 3 cut(s) 788, 877, 980
BseMI GCAATG 1 cut(s) 91
BseNI ACTGG 3 cut(s) 186, 212, 449
BseRI GAGGAG 1 cut(s) 384
BseXI GCAGC 2 cut(s) 814, 904
Bsh1236I CGCG 1 cut(s) 407
Bsh1285I CGRYCG 1 cut(s) 345
BshFI GGCC 1 cut(s) 310
BshNI GGYRCC 1 cut(s) 808
BsiEI CGRYCG 1 cut(s) 345
BsiHKAI GWGCWC 1 cut(s) 910
BsiHKCI CYCGRG 1 cut(s) 396
BslFI GGGAC 1 cut(s) 584
BslI CCNNNNNNNGG 3 cut(s) 788, 877, 980
BsmAI GTCTC 3 cut(s) 35, 1013, 1056
BsmFI GGGAC 1 cut(s) 584
BsmI GAATGC 1 cut(s) 178
BsnI GGCC 1 cut(s) 310
BsoBI CYCGRG 1 cut(s) 396
Bsp1286I GDGCHC 1 cut(s) 910
Bsp143I GATC 4 cut(s) 23, 577, 867, 1159
Bsp19I CCATGG 2 cut(s) 336, 458
BspACI CCGC 8 cut(s) 233, 341, 407, 696, 750, 947, 996, 1012
BspANI GGCC 1 cut(s) 310
BspFNI CGCG 1 cut(s) 407
BspLI GGNNCC 1 cut(s) 810
BspMI ACCTGC 1 cut(s) 661
BspPI GGATC 2 cut(s) 862, 1154
BspT107I GGYRCC 1 cut(s) 808
BsrBI CCGCTC 1 cut(s) 698
BsrDI GCAATG 1 cut(s) 91
BsrI ACTGG 3 cut(s) 186, 212, 449
BssECI CCNNGG 2 cut(s) 336, 458
BssMI GATC 4 cut(s) 23, 577, 867, 1159
BssSI CACGAG 1 cut(s) 1053
BssT1I CCWWGG 2 cut(s) 336, 458
Bst2BI CACGAG 1 cut(s) 1053
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 1 cut(s) 155
Bst6I CTCTTC 2 cut(s) 624, 1100
BstC8I GCNNGC 1 cut(s) 752
BstDSI CCRYGG 2 cut(s) 336, 458
BstEII GGTNACC 1 cut(s) 914
BstF5I GGATG 4 cut(s) 529, 685, 1089, 1147
BstFNI CGCG 1 cut(s) 407
BstH2I RGCGCY 1 cut(s) 1008
BstHHI GCGC 1 cut(s) 1007
BstKTI GATC 4 cut(s) 26, 580, 870, 1162
BstMAI GTCTC 3 cut(s) 35, 1013, 1056
BstMBI GATC 4 cut(s) 23, 577, 867, 1159
BstMCI CGRYCG 1 cut(s) 345
BstMWI GCNNNNNNNGC 4 cut(s) 95, 361, 526, 1004
BstNI CCWGG 1 cut(s) 263
BstNSI RCATGY 2 cut(s) 195, 1152
BstPI GGTNACC 1 cut(s) 914
BstSCI CCNGG 1 cut(s) 261
BstSFI CTRYAG 1 cut(s) 34
BstUI CGCG 1 cut(s) 407
BstV1I GCAGC 2 cut(s) 814, 904
BstV2I GAAGAC 1 cut(s) 962
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BsuI GTATCC 2 cut(s) 465, 880
BsuRI GGCC 1 cut(s) 310
BtgI CCRYGG 2 cut(s) 336, 458
BtgZI GCGATG 1 cut(s) 769
BtsCI GGATG 4 cut(s) 529, 685, 1089, 1147
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 3 cut(s) 51, 135, 219
BveI ACCTGC 1 cut(s) 661
Cac8I GCNNGC 1 cut(s) 752
CfoI GCGC 1 cut(s) 1007
Csp6I GTAC 5 cut(s) 156, 189, 219, 550, 952
CviAII CATG 9 cut(s) 192, 337, 412, 459, 470, 479, 831, 872, 1149
CviJI RGCY 8 cut(s) 211, 310, 335, 364, 637, 827, 895, 1133
CviKI_1 RGCY 8 cut(s) 211, 310, 335, 364, 637, 827, 895, 1133
CviQI GTAC 5 cut(s) 156, 189, 219, 550, 952
DpnI GATC 4 cut(s) 25, 579, 869, 1161
DpnII GATC 4 cut(s) 23, 577, 867, 1159
DraI TTTAAA 1 cut(s) 109
Eam1104I CTCTTC 2 cut(s) 624, 1100
EarI CTCTTC 2 cut(s) 624, 1100
Eco130I CCWWGG 2 cut(s) 336, 458
Eco147I AGGCCT 1 cut(s) 310
Eco32I GATATC 2 cut(s) 611, 645
Eco47III AGCGCT 1 cut(s) 1006
Eco57I CTGAAG 2 cut(s) 143, 243
Eco88I CYCGRG 1 cut(s) 396
Eco91I GGTNACC 1 cut(s) 914
EcoO65I GGTNACC 1 cut(s) 914
EcoRII CCWGG 1 cut(s) 261
EcoRV GATATC 2 cut(s) 611, 645
EcoT14I CCWWGG 2 cut(s) 336, 458
EcoT22I ATGCAT 1 cut(s) 150
ErhI CCWWGG 2 cut(s) 336, 458
FaeI CATG 9 cut(s) 195, 340, 415, 462, 473, 482, 834, 875, 1152
FalI AAGNNNNNCTT 4 cut(s) 509, 541, 621, 653
FaqI GGGAC 1 cut(s) 584
FatI CATG 9 cut(s) 191, 336, 411, 458, 469, 478, 830, 871, 1148
FauI CCCGC 1 cut(s) 743
Fnu4HI GCNGC 2 cut(s) 828, 893
FokI GGATG 4 cut(s) 516, 672, 1096, 1154
Fsp4HI GCNGC 2 cut(s) 828, 893
FspBI CTAG 2 cut(s) 365, 822
GlaI GCGC 1 cut(s) 1006
GluI GCNGC 2 cut(s) 828, 893
HaeII RGCGCY 1 cut(s) 1008
HaeIII GGCC 1 cut(s) 310
HhaI GCGC 1 cut(s) 1007
Hin1II CATG 9 cut(s) 195, 340, 415, 462, 473, 482, 834, 875, 1152
Hin6I GCGC 1 cut(s) 1005
HinP1I GCGC 1 cut(s) 1005
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 8 cut(s) 475, 493, 522, 853, 1025, 1040, 1049, 1110
HpaI GTTAAC 1 cut(s) 370
HphI GGTGA 2 cut(s) 40, 926
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 3 cut(s) 60, 676, 858
Hpy188III TCNNGA 7 cut(s) 247, 398, 533, 659, 822, 884, 1055
Hpy8I GTNNAC 1 cut(s) 370
Hpy99I CGWCG 1 cut(s) 705
HpyAV CCTTC 1 cut(s) 300
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4IV ACGT 1 cut(s) 1120
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 361, 526, 1004
HpySE526I ACGT 1 cut(s) 1120
Hsp92II CATG 9 cut(s) 195, 340, 415, 462, 473, 482, 834, 875, 1152
HspAI GCGC 1 cut(s) 1005
KspAI GTTAAC 1 cut(s) 370
Kzo9I GATC 4 cut(s) 23, 577, 867, 1159
LmnI GCTCC 1 cut(s) 361
LpnPI CCDG 9 cut(s) 199, 225, 248, 275, 430, 666, 825, 869, 1086
Lsp1109I GCAGC 2 cut(s) 814, 904
LweI GCATC 6 cut(s) 135, 156, 538, 691, 795, 934
MaeI CTAG 2 cut(s) 365, 822
MaeII ACGT 1 cut(s) 1120
MaeIII GTNAC 1 cut(s) 914
MalI GATC 4 cut(s) 25, 579, 869, 1161
MbiI CCGCTC 1 cut(s) 698
MboI GATC 4 cut(s) 23, 577, 867, 1159
MboII GAAGA 6 cut(s) 587, 641, 738, 962, 1117, 1138
MflI RGATCY 1 cut(s) 577
MhlI GDGCHC 1 cut(s) 910
MluCI AATT 3 cut(s) 199, 240, 957
MlyI GAGTC 2 cut(s) 1019, 1058
MmeI TCCRAC 2 cut(s) 998, 1103
MnlI CCTC 7 cut(s) 402, 405, 612, 625, 789, 1074, 1131
Mph1103I ATGCAT 1 cut(s) 150
MroXI GAANNNNTTC 1 cut(s) 1109
MseI TTAA 3 cut(s) 44, 108, 369
MslI CAYNNNNRTG 2 cut(s) 426, 835
MspR9I CCNGG 1 cut(s) 263
Mva1269I GAATGC 1 cut(s) 178
MvaI CCWGG 1 cut(s) 263
MvnI CGCG 1 cut(s) 407
MwoI GCNNNNNNNGC 4 cut(s) 95, 361, 526, 1004
NcoI CCATGG 2 cut(s) 336, 458
NdeII GATC 4 cut(s) 23, 577, 867, 1159
NlaIII CATG 9 cut(s) 195, 340, 415, 462, 473, 482, 834, 875, 1152
NlaIV GGNNCC 1 cut(s) 810
NmuCI GTSAC 1 cut(s) 914
NsiI ATGCAT 1 cut(s) 150
NspI RCATGY 2 cut(s) 195, 1152
PaeR7I CTCGAG 1 cut(s) 396
PceI AGGCCT 1 cut(s) 310
PciI ACATGT 1 cut(s) 1148
PctI GAATGC 1 cut(s) 178
PdmI GAANNNNTTC 1 cut(s) 1109
PfeI GAWTC 6 cut(s) 475, 493, 522, 853, 1040, 1110
PkrI GCNGC 2 cut(s) 829, 894
PleI GAGTC 2 cut(s) 1019, 1057
PpsI GAGTC 2 cut(s) 1019, 1057
PscI ACATGT 1 cut(s) 1148
Psp1406I AACGTT 1 cut(s) 1120
Psp6I CCWGG 1 cut(s) 261
PspEI GGTNACC 1 cut(s) 914
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 1 cut(s) 810
PsuI RGATCY 1 cut(s) 577
RsaI GTAC 5 cut(s) 157, 190, 220, 551, 953
RsaNI GTAC 5 cut(s) 156, 189, 219, 550, 952
RseI CAYNNNNRTG 2 cut(s) 426, 835
SaqAI TTAA 3 cut(s) 44, 108, 369
SatI GCNGC 2 cut(s) 828, 893
Sau3AI GATC 4 cut(s) 23, 577, 867, 1159
ScaI AGTACT 1 cut(s) 157
SchI GAGTC 2 cut(s) 1019, 1058
ScrFI CCNGG 1 cut(s) 263
SduI GDGCHC 1 cut(s) 910
SfaNI GCATC 6 cut(s) 135, 156, 538, 691, 795, 934
SfcI CTRYAG 1 cut(s) 34
Sfr274I CTCGAG 1 cut(s) 396
SlaI CTCGAG 1 cut(s) 396
SmiMI CAYNNNNRTG 2 cut(s) 426, 835
SmlI CTYRAG 3 cut(s) 396, 638, 909
SmoI CTYRAG 3 cut(s) 396, 638, 909
Sse9I AATT 3 cut(s) 199, 240, 957
SseBI AGGCCT 1 cut(s) 310
SsiI CCGC 8 cut(s) 233, 341, 407, 696, 750, 947, 996, 1012
SspMI CTAG 2 cut(s) 365, 822
StuI AGGCCT 1 cut(s) 310
StyD4I CCNGG 1 cut(s) 261
StyI CCWWGG 2 cut(s) 336, 458
TaaI ACNGT 1 cut(s) 155
TaiI ACGT 1 cut(s) 1123
TaqI TCGA 7 cut(s) 246, 257, 320, 397, 438, 703, 1028
TaqII GACCGA 1 cut(s) 933
TasI AATT 3 cut(s) 199, 240, 957
TatI WGTACW 3 cut(s) 155, 188, 951
TfiI GAWTC 6 cut(s) 475, 493, 522, 853, 1040, 1110
Tru1I TTAA 3 cut(s) 44, 108, 369
Tru9I TTAA 3 cut(s) 44, 108, 369
TscAI CASTG 3 cut(s) 58, 142, 219
TseFI GTSAC 1 cut(s) 914
TseI GCWGC 2 cut(s) 827, 892
Tsp45I GTSAC 1 cut(s) 914
TspGWI ACGGA 3 cut(s) 475, 1052, 1152
TspRI CASTG 3 cut(s) 58, 142, 219
XapI RAATTY 1 cut(s) 199
XbaI TCTAGA 1 cut(s) 821
XceI RCATGY 2 cut(s) 195, 1152
XhoI CTCGAG 1 cut(s) 396
XmnI GAANNNNTTC 1 cut(s) 1109
XspI CTAG 2 cut(s) 365, 822
ZrmI AGTACT 1 cut(s) 157
Zsp2I ATGCAT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.