MD08G1245700.v1.1

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004588
Physical Location & Seq
Forward (+)
31020054 .. 31026825
6772 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1245700.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGATCAACCCTGCCGCTGTGCTGTCGGACCCGAAGAATAGGTCATTCTACTGCAGTCTCTTCCTTGTAGCTTCTTTGATCTGCGGGGCTTACTTCATCGGTGGTGCAACCATTGCTCACGAGTACAAGGAAAAATTAACAAGATGGAAAGTGGTCTACAATAGGCAGAATACAAATTTTCATACGTGCAGGAATCGTTGCCAACCTTCAGGGAGTGAGGCATTACCGGAAGGAATTATTGCCAAAACATCGGACTTGGAACCGCTGCCTCTATGGGGTTCATCTGTGAACAATGAAAACTCAAAACCTTCTAAGAGCTTGCTGGCAATTGCAGTTGGAATAAGGCAGAAAGAAATAGTGGACAAAATTGTCAAAAAGTTTCTATCAAGTGATTTCGTTGTTATGCTTTTTCATTATGATGGTGCGGTGGATAAATGGAGGGATTTACCTTGGAGTGATCGTGCCATACACGTGTCTGTGATGAATCAAACAAAATGGTGGTTCGCCAAACGTTTCCTGCATCCAGACATAGTTTCCGAGTACAAATATATTTTTCTTTGGGATGAGGACCTTGGAGTTGAGAATTTTAACCCAAAACGATATGTATCCATTGTTCAGGAAGAGGTACTTGAAATATCACAGCCAGCACTTGATCCTGACAAGTCAGAGGTGTATCATCCAATCACTGCACGTGTGAAGAAATCAAAAGTGCACAGGTTTCATAAGTATAAAGGCAGTGCAAGGTGTGACGACCATAGTTCTGCTCCTCCTTGTGCAGGTTGGGTGGAAATGATGGCACCTGTATTCTCAAGATCTGCATGGCAATGTGTATGGTACATGATCCAGAATGATCTGGTCCATGCATGGGGCCTGGATGTGCAGCTTGGTTATTGTGCACAAGGAGATCGAACACAAAACGTTGGTGTGGTTGACTCAGAGTATATAGTTCATCTTGGTCTTTCTACGCTCGGTGTCTCAGATGGCAATAAGGTGATTAATAGCCCGGATCCTTCTCAGAAAGGGGACTCGAAAGCATTGGCACCATCTTCCTCGGATAAAGTCAATGATCGATCTAAAGTTAGGATGCAGTCCTTTATTGACATGCAGATCTTTAAGGAAAGATGGAGTGCTGCAGTAAAGGAGGATAAATGTTGGGTTGACCCATATCAACTATCAACAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

395

Amino Acids

44.76

Weight (kDa)

8.25

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 67 - 381 8.1e-143 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 368
Acc36I ACCTGC 1 cut(s) 767
AccB1I GGYRCC 2 cut(s) 796, 1039
AccI GTMKAC 1 cut(s) 156
AciI CCGC 4 cut(s) 15, 84, 263, 425
AclI AACGTT 2 cut(s) 511, 918
AclWI GGATC 4 cut(s) 647, 835, 1001, 1014
AcsI RAATTY 2 cut(s) 175, 583
AcuI CTGAAG 1 cut(s) 192
AcvI CACGTG 2 cut(s) 472, 692
AfaI GTAC 4 cut(s) 125, 542, 627, 836
AfiI CCNNNNNNNGG 3 cut(s) 275, 776, 865
AflIII ACRYGT 3 cut(s) 469, 471, 691
AgsI TTSAA 1 cut(s) 632
AjnI CCWGG 1 cut(s) 870
AluBI AGCT 3 cut(s) 71, 318, 883
AluI AGCT 3 cut(s) 71, 318, 883
Alw21I GWGCWC 2 cut(s) 714, 898
Alw26I GTCTC 2 cut(s) 62, 979
Alw44I GTGCAC 2 cut(s) 710, 894
AlwI GGATC 4 cut(s) 647, 835, 1001, 1014
AoxI GGCC 1 cut(s) 868
ApaLI GTGCAC 2 cut(s) 710, 894
ApeKI GCWGC 3 cut(s) 265, 880, 1130
ApoI RAATTY 2 cut(s) 175, 583
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AseI ATTAAT 1 cut(s) 996
AspS9I GGNCC 4 cut(s) 28, 568, 856, 868
AsuC2I CCSGG 1 cut(s) 1004
AsuHPI GGTGA 1 cut(s) 1003
AvaII GGWCC 3 cut(s) 28, 568, 856
BaeGI GKGCMC 2 cut(s) 714, 898
BamHI GGATCC 1 cut(s) 1006
BanI GGYRCC 2 cut(s) 796, 1039
BauI CACGAG 1 cut(s) 119
BbrPI CACGTG 2 cut(s) 472, 692
Bbv12I GWGCWC 2 cut(s) 714, 898
BbvI GCAGC 3 cut(s) 252, 892, 1117
BccI CCATC 6 cut(s) 138, 413, 787, 974, 1051, 1116
BciT130I CCWGG 1 cut(s) 872
BciVI GTATCC 1 cut(s) 616
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 1004
BcoDI GTCTC 2 cut(s) 62, 979
BfmI CTRYAG 2 cut(s) 52, 1131
BfuAI ACCTGC 1 cut(s) 767
BfuI GTATCC 1 cut(s) 616
BglII AGATCT 2 cut(s) 812, 1107
BisI GCNGC 4 cut(s) 15, 266, 881, 1131
BlsI GCNGC 4 cut(s) 16, 267, 882, 1132
Bme1390I CCNGG 2 cut(s) 872, 1004
Bme18I GGWCC 3 cut(s) 28, 568, 856
BmgT120I GGNCC 4 cut(s) 28, 568, 856, 868
BmiI GGNNCC 6 cut(s) 30, 261, 798, 869, 1008, 1041
BmrFI CCNGG 2 cut(s) 872, 1004
BmsI GCATC 2 cut(s) 529, 1074
BpuEI CTTGAG 1 cut(s) 793
BpuMI CCSGG 1 cut(s) 1004
Bsa29I ATCGAT 1 cut(s) 1069
BsaAI YACGTR 3 cut(s) 186, 472, 692
BsaBI GATNNNNATC 1 cut(s) 604
BsaJI CCNNGG 3 cut(s) 449, 571, 1050
BsaWI WCCGGW 1 cut(s) 226
BsaXI ACNNNNNCTCC 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 3 cut(s) 275, 776, 865
Bse3DI GCAATG 2 cut(s) 111, 830
Bse8I GATNNNNATC 1 cut(s) 604
BseBI CCWGG 1 cut(s) 872
BseCI ATCGAT 1 cut(s) 1069
BseDI CCNNGG 3 cut(s) 449, 571, 1050
BseGI GGATG 5 cut(s) 520, 568, 676, 880, 1089
BseJI GATNNNNATC 1 cut(s) 604
BseLI CCNNNNNNNGG 3 cut(s) 275, 776, 865
BseMI GCAATG 2 cut(s) 111, 830
BseMII CTCAG 3 cut(s) 948, 990, 1028
BseRI GAGGAG 1 cut(s) 756
BseSI GKGCMC 2 cut(s) 714, 898
BseXI GCAGC 3 cut(s) 252, 892, 1117
BsgI GTGCAG 4 cut(s) 208, 672, 795, 899
BshFI GGCC 1 cut(s) 870
BshNI GGYRCC 2 cut(s) 796, 1039
BshVI ATCGAT 1 cut(s) 1069
BsiHKAI GWGCWC 2 cut(s) 714, 898
BsiSI CCGG 2 cut(s) 227, 1004
BslFI GGGAC 1 cut(s) 1037
BslI CCNNNNNNNGG 3 cut(s) 275, 776, 865
BsmAI GTCTC 2 cut(s) 62, 979
BsmFI GGGAC 1 cut(s) 1037
BsnI GGCC 1 cut(s) 870
Bsp1286I GDGCHC 2 cut(s) 714, 898
BspACI CCGC 4 cut(s) 15, 84, 263, 425
BspANI GGCC 1 cut(s) 870
BspCNI CTCAG 3 cut(s) 947, 989, 1027
BspDI ATCGAT 1 cut(s) 1069
BspLI GGNNCC 6 cut(s) 30, 261, 798, 869, 1008, 1041
BspMAI CTGCAG 2 cut(s) 56, 1135
BspMI ACCTGC 1 cut(s) 767
BspPI GGATC 4 cut(s) 647, 835, 1001, 1014
BspT107I GGYRCC 2 cut(s) 796, 1039
BsrDI GCAATG 2 cut(s) 111, 830
BssECI CCNNGG 3 cut(s) 449, 571, 1050
BssSI CACGAG 1 cut(s) 119
BssT1I CCWWGG 2 cut(s) 449, 571
Bst2BI CACGAG 1 cut(s) 119
Bst2UI CCWGG 1 cut(s) 872
Bst6I CTCTTC 2 cut(s) 65, 615
BstAPI GCANNNNNTGC 1 cut(s) 113
BstBAI YACGTR 3 cut(s) 186, 472, 692
BstC8I GCNNGC 3 cut(s) 320, 324, 645
BstDEI CTNAG 4 cut(s) 312, 934, 976, 1014
BstENI CCTNNNNNAGG 1 cut(s) 774
BstF5I GGATG 5 cut(s) 520, 568, 676, 880, 1089
BstMAI GTCTC 2 cut(s) 62, 979
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstNI CCWGG 1 cut(s) 872
BstNSI RCATGY 1 cut(s) 1105
BstSCI CCNGG 2 cut(s) 870, 1002
BstSFI CTRYAG 2 cut(s) 52, 1131
BstSLI GKGCMC 2 cut(s) 714, 898
BstV1I GCAGC 3 cut(s) 252, 892, 1117
BstX2I RGATCY 3 cut(s) 812, 1006, 1107
BstYI RGATCY 3 cut(s) 812, 1006, 1107
Bsu15I ATCGAT 1 cut(s) 1069
BsuI GTATCC 1 cut(s) 616
BsuRI GGCC 1 cut(s) 870
BsuTUI ATCGAT 1 cut(s) 1069
BtsCI GGATG 5 cut(s) 520, 568, 676, 880, 1089
BtsI GCAGTG 2 cut(s) 684, 742
BtsIMutI CAGTG 2 cut(s) 684, 742
BveI ACCTGC 1 cut(s) 767
Cac8I GCNNGC 3 cut(s) 320, 324, 645
Cfr13I GGNCC 4 cut(s) 28, 568, 856, 868
ClaI ATCGAT 1 cut(s) 1069
Csp6I GTAC 4 cut(s) 124, 541, 626, 835
CviAII CATG 5 cut(s) 819, 838, 860, 864, 1102
CviJI RGCY 7 cut(s) 71, 89, 318, 643, 870, 883, 1002
CviKI_1 RGCY 7 cut(s) 71, 89, 318, 643, 870, 883, 1002
CviQI GTAC 4 cut(s) 124, 541, 626, 835
DdeI CTNAG 4 cut(s) 312, 934, 976, 1014
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
Eam1104I CTCTTC 2 cut(s) 65, 615
EarI CTCTTC 2 cut(s) 65, 615
Eco130I CCWWGG 2 cut(s) 449, 571
Eco47I GGWCC 3 cut(s) 28, 568, 856
Eco57I CTGAAG 1 cut(s) 192
Eco72I CACGTG 2 cut(s) 472, 692
EcoNI CCTNNNNNAGG 1 cut(s) 774
EcoO109I RGGNCCY 2 cut(s) 568, 868
EcoRII CCWGG 1 cut(s) 870
EcoT14I CCWWGG 2 cut(s) 449, 571
EcoT22I ATGCAT 1 cut(s) 865
ErhI CCWWGG 2 cut(s) 449, 571
FaeI CATG 5 cut(s) 822, 841, 863, 867, 1105
FalI AAGNNNNNCTT 2 cut(s) 612, 644
FaqI GGGAC 1 cut(s) 1037
FatI CATG 5 cut(s) 818, 837, 859, 863, 1101
FauI CCCGC 1 cut(s) 77
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 4 cut(s) 15, 266, 881, 1131
FokI GGATG 5 cut(s) 507, 575, 663, 887, 1096
Fsp4HI GCNGC 4 cut(s) 15, 266, 881, 1131
GluI GCNGC 4 cut(s) 15, 266, 881, 1131
HaeIII GGCC 1 cut(s) 870
HapII CCGG 2 cut(s) 227, 1004
Hin1II CATG 5 cut(s) 822, 841, 863, 867, 1105
HincII GTYRAC 2 cut(s) 931, 1159
HindII GTYRAC 2 cut(s) 931, 1159
HinfI GANTC 4 cut(s) 193, 484, 932, 1025
HpaII CCGG 2 cut(s) 227, 1004
HphI GGTGA 1 cut(s) 1003
Hpy166II GTNNAC 7 cut(s) 157, 289, 361, 712, 896, 931, 1159
Hpy188I TCNGA 8 cut(s) 28, 253, 538, 667, 937, 979, 1017, 1054
Hpy188III TCNNGA 6 cut(s) 119, 524, 617, 656, 810, 844
Hpy8I GTNNAC 7 cut(s) 157, 289, 361, 712, 896, 931, 1159
HpyAV CCTTC 4 cut(s) 216, 224, 318, 1020
HpyCH4IV ACGT 5 cut(s) 185, 471, 511, 691, 918
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpyF3I CTNAG 4 cut(s) 312, 934, 976, 1014
HpySE526I ACGT 5 cut(s) 185, 471, 511, 691, 918
Hsp92II CATG 5 cut(s) 822, 841, 863, 867, 1105
Ksp22I TGATCA 1 cut(s) 3
LmnI GCTCC 1 cut(s) 769
Lsp1109I GCAGC 3 cut(s) 252, 892, 1117
LweI GCATC 2 cut(s) 529, 1074
MaeII ACGT 5 cut(s) 185, 471, 511, 691, 918
MaeIII GTNAC 1 cut(s) 746
MboII GAAGA 5 cut(s) 46, 52, 632, 709, 1038
MfeI CAATTG 1 cut(s) 327
MflI RGATCY 3 cut(s) 812, 1006, 1107
MhlI GDGCHC 2 cut(s) 714, 898
MluCI AATT 6 cut(s) 134, 175, 234, 327, 366, 583
MlyI GAGTC 2 cut(s) 926, 1019
MmeI TCCRAC 2 cut(s) 6, 316
MnlI CCTC 9 cut(s) 211, 279, 432, 559, 616, 661, 777, 1060, 1135
Mph1103I ATGCAT 1 cut(s) 865
MseI TTAA 4 cut(s) 137, 588, 996, 1113
MslI CAYNNNNRTG 3 cut(s) 417, 470, 823
MspA1I CMGCKG 2 cut(s) 17, 265
MspI CCGG 2 cut(s) 227, 1004
MspR9I CCNGG 2 cut(s) 872, 1004
MunI CAATTG 1 cut(s) 327
MvaI CCWGG 1 cut(s) 872
MwoI GCNNNNNNNGC 1 cut(s) 113
NciI CCSGG 1 cut(s) 1004
NlaIII CATG 5 cut(s) 822, 841, 863, 867, 1105
NlaIV GGNNCC 6 cut(s) 30, 261, 798, 869, 1008, 1041
NmuCI GTSAC 1 cut(s) 746
NsiI ATGCAT 1 cut(s) 865
NspI RCATGY 1 cut(s) 1105
PfeI GAWTC 2 cut(s) 193, 484
PkrI GCNGC 4 cut(s) 16, 267, 882, 1132
PleI GAGTC 2 cut(s) 926, 1019
PmaCI CACGTG 2 cut(s) 472, 692
PmlI CACGTG 2 cut(s) 472, 692
PpsI GAGTC 2 cut(s) 926, 1019
Ppu21I YACGTR 3 cut(s) 186, 472, 692
PpuMI RGGWCCY 1 cut(s) 568
PshBI ATTAAT 1 cut(s) 996
Psp1406I AACGTT 2 cut(s) 511, 918
Psp5II RGGWCCY 1 cut(s) 568
Psp6I CCWGG 1 cut(s) 870
PspCI CACGTG 2 cut(s) 472, 692
PspGI CCWGG 1 cut(s) 870
PspN4I GGNNCC 6 cut(s) 30, 261, 798, 869, 1008, 1041
PspPI GGNCC 4 cut(s) 28, 568, 856, 868
PspPPI RGGWCCY 1 cut(s) 568
PsrI GAACNNNNNNTAC 2 cut(s) 597, 629
PstI CTGCAG 2 cut(s) 56, 1135
PsuI RGATCY 3 cut(s) 812, 1006, 1107
RsaI GTAC 4 cut(s) 125, 542, 627, 836
RsaNI GTAC 4 cut(s) 124, 541, 626, 835
RseI CAYNNNNRTG 3 cut(s) 417, 470, 823
SaqAI TTAA 4 cut(s) 137, 588, 996, 1113
SatI GCNGC 4 cut(s) 15, 266, 881, 1131
Sau96I GGNCC 4 cut(s) 28, 568, 856, 868
SchI GAGTC 2 cut(s) 926, 1019
ScrFI CCNGG 2 cut(s) 872, 1004
SduI GDGCHC 2 cut(s) 714, 898
SfaNI GCATC 2 cut(s) 529, 1074
SfcI CTRYAG 2 cut(s) 52, 1131
SinI GGWCC 3 cut(s) 28, 568, 856
SmiMI CAYNNNNRTG 3 cut(s) 417, 470, 823
SmlI CTYRAG 1 cut(s) 808
SmoI CTYRAG 1 cut(s) 808
Sse9I AATT 6 cut(s) 134, 175, 234, 327, 366, 583
SsiI CCGC 4 cut(s) 15, 84, 263, 425
StyD4I CCNGG 2 cut(s) 870, 1002
StyI CCWWGG 2 cut(s) 449, 571
TaiI ACGT 5 cut(s) 188, 474, 514, 694, 921
TaqI TCGA 3 cut(s) 907, 1028, 1069
TasI AATT 6 cut(s) 134, 175, 234, 327, 366, 583
TatI WGTACW 2 cut(s) 123, 540
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 2 cut(s) 193, 484
Tru1I TTAA 4 cut(s) 137, 588, 996, 1113
Tru9I TTAA 4 cut(s) 137, 588, 996, 1113
TscAI CASTG 2 cut(s) 691, 742
TseFI GTSAC 1 cut(s) 746
TseI GCWGC 3 cut(s) 265, 880, 1130
Tsp45I GTSAC 1 cut(s) 746
TspDTI ATGAA 8 cut(s) 85, 170, 270, 309, 401, 497, 710, 938
TspRI CASTG 2 cut(s) 691, 742
VneI GTGCAC 2 cut(s) 710, 894
VpaK11BI GGWCC 3 cut(s) 28, 568, 856
VspI ATTAAT 1 cut(s) 996
XagI CCTNNNNNAGG 1 cut(s) 774
XapI RAATTY 2 cut(s) 175, 583
XceI RCATGY 1 cut(s) 1105
XmiI GTMKAC 1 cut(s) 156
Zsp2I ATGCAT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.