MD15G1440900.v1.1

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004588
Physical Location & Seq
Forward (+)
54067203 .. 54073494
6292 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1440900.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGATTAATCCTGCCTCTACGCTGTCAGACCCAAAGAATAGGTCATTCTACTGCTGTCTCGTCATTGTATCTTCATTGATCTGCGGGGCTTACTTCATCGGTGGTGCATCCATTGCTCAGGAGTATAAGGAAAAATTAACAAGATGGAAAGTGATCTACAATACGCAGAATACAACTTTGCATACATGCAAGAATCGATGCCAACCTTCAGGGAGTGAGGCATTACCGAAAGGAATTGTTGTCAAAACATCGGACTTGGAAGCGCTGCCTTTATGGGGTTCATCTGTGAAAAATGAAAACTCAAAACCTTCTAAGAGCTTGCTGGCAATTGCAGTTGGAATAAAGCAGAAAGAGATAGTGGACAAAATTGTCAAAAAGTTTCTATCAAGTGATTTTGTTGTAATGCTTTTTCATTATGATGGTGCGGTGGATAAATGGAGGGATTTACCTTGGAGTGATCATGCCATACACGTGTCTGTGATGAATCAAACAAAATGGTGGTTCGCCAAACGTTTCCTGCATCCAGATATAGTTTCGGAGTACAAATATATTTTTCTTTGGGATGAGGACCTTGGAGTTGAGAATTTTGACCCGATGCGATATCTATCCATAATTCAAGAAGAGGGACTGGAAATATCACAGCCGGCACTTGATCCTGACAAGTCAGAGGTGTATCATCCAATCACTGCACGTGTGAAGAATTCAAAAGTGCACAGACGGTTTTATAAGTATAAGGGCAGTGGAAGGTGTGACGACCATAGCTCTGCTCCTCCTTGTGCAGGTTGGGTGGAAATGATGGCACCTGTATTTTCAAGAACTGCTTGGCAATGTGTATGGTACATGATCCAGAATGATTTGGTCCATGCATGGGGCCTGGATGTGCAGCTTGGTTATTGTGCACAAGGAGATCGAACACAAAACGTTGGTGTGGTTGACTCAGAGTATATAGTTCATCTTGGTCTTCCTACGCTTGGTGTCTCAGATGGAAATAAGGTGACTAATTCCCCAGATCCTTCTCAGAAAGGGGACTCGAAAGCATTGGCCCCATCTGCCTCCGATAAAGTCAATGATAGATCTAAAGTTAGGATGCAGTCCTTTATTGATATGCAGATCTTTAAGGAAAGATGGAGTAATGCAGTAAAGGAGGAAAAATGTTGGGTTGACCCGTATCAACTAACAACAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

396

Amino Acids

44.79

Weight (kDa)

7.94

Isoelectric Point (pI)

32.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 67 - 382 7.9e-147 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 726
AasI GACNNNNNNGTC 1 cut(s) 368
Acc36I ACCTGC 1 cut(s) 770
AccB1I GGYRCC 1 cut(s) 799
AciI CCGC 2 cut(s) 84, 425
AclI AACGTT 2 cut(s) 511, 921
AclWI GGATC 3 cut(s) 647, 838, 1004
AcsI RAATTY 2 cut(s) 583, 700
AcuI CTGAAG 1 cut(s) 192
AcvI CACGTG 2 cut(s) 472, 692
AfaI GTAC 2 cut(s) 542, 839
AfeI AGCGCT 1 cut(s) 264
AfiI CCNNNNNNNGG 4 cut(s) 275, 779, 868, 971
AflIII ACRYGT 3 cut(s) 469, 471, 691
AgsI TTSAA 3 cut(s) 617, 705, 813
AjnI CCWGG 1 cut(s) 873
AluBI AGCT 3 cut(s) 318, 762, 886
AluI AGCT 3 cut(s) 318, 762, 886
Alw21I GWGCWC 2 cut(s) 714, 901
Alw26I GTCTC 2 cut(s) 62, 982
Alw44I GTGCAC 2 cut(s) 710, 897
AlwI GGATC 3 cut(s) 647, 838, 1004
Aor51HI AGCGCT 1 cut(s) 264
AoxI GGCC 2 cut(s) 871, 1041
ApaLI GTGCAC 2 cut(s) 710, 897
ApeKI GCWGC 2 cut(s) 265, 883
ApoI RAATTY 2 cut(s) 583, 700
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AseI ATTAAT 1 cut(s) 6
AspLEI GCGC 1 cut(s) 265
AspS9I GGNCC 4 cut(s) 568, 859, 871, 1042
AsuHPI GGTGA 1 cut(s) 1006
AvaII GGWCC 2 cut(s) 568, 859
BaeGI GKGCMC 2 cut(s) 714, 901
BanI GGYRCC 1 cut(s) 799
BbrPI CACGTG 2 cut(s) 472, 692
BbsI GAAGAC 1 cut(s) 953
Bbv12I GWGCWC 2 cut(s) 714, 901
BbvI GCAGC 2 cut(s) 252, 895
BccI CCATC 6 cut(s) 138, 413, 790, 977, 1054, 1119
BciT130I CCWGG 1 cut(s) 875
BclI TGATCA 1 cut(s) 457
BcoDI GTCTC 2 cut(s) 62, 982
BfoI RGCGCY 1 cut(s) 266
BfuAI ACCTGC 1 cut(s) 770
BglII AGATCT 2 cut(s) 1073, 1110
BisI GCNGC 2 cut(s) 266, 884
BlsI GCNGC 2 cut(s) 267, 885
Bme1390I CCNGG 1 cut(s) 875
Bme18I GGWCC 2 cut(s) 568, 859
BmgT120I GGNCC 4 cut(s) 568, 859, 871, 1042
BmiI GGNNCC 3 cut(s) 801, 872, 1044
BmrFI CCNGG 1 cut(s) 875
BmsI GCATC 5 cut(s) 116, 188, 529, 585, 1077
BpiI GAAGAC 1 cut(s) 953
Bpu10I CCTNAGC 1 cut(s) 117
Bsa29I ATCGAT 1 cut(s) 196
BsaAI YACGTR 2 cut(s) 472, 692
BsaBI GATNNNNATC 1 cut(s) 604
BsaJI CCNNGG 2 cut(s) 449, 571
Bsc4I CCNNNNNNNGG 4 cut(s) 275, 779, 868, 971
Bse118I RCCGGY 1 cut(s) 643
Bse1I ACTGG 1 cut(s) 633
Bse3DI GCAATG 2 cut(s) 111, 833
Bse8I GATNNNNATC 1 cut(s) 604
BseBI CCWGG 1 cut(s) 875
BseCI ATCGAT 1 cut(s) 196
BseDI CCNNGG 2 cut(s) 449, 571
BseGI GGATG 6 cut(s) 107, 520, 568, 676, 883, 1092
BseJI GATNNNNATC 1 cut(s) 604
BseLI CCNNNNNNNGG 4 cut(s) 275, 779, 868, 971
BseMI GCAATG 2 cut(s) 111, 833
BseMII CTCAG 4 cut(s) 131, 951, 993, 1031
BseNI ACTGG 1 cut(s) 633
BseRI GAGGAG 1 cut(s) 759
BseSI GKGCMC 2 cut(s) 714, 901
BseXI GCAGC 2 cut(s) 252, 895
BsgI GTGCAG 3 cut(s) 672, 798, 902
BshFI GGCC 2 cut(s) 873, 1043
BshNI GGYRCC 1 cut(s) 799
BshVI ATCGAT 1 cut(s) 196
BsiHKAI GWGCWC 2 cut(s) 714, 901
BsiSI CCGG 1 cut(s) 644
BslFI GGGAC 2 cut(s) 639, 1040
BslI CCNNNNNNNGG 4 cut(s) 275, 779, 868, 971
BsmAI GTCTC 2 cut(s) 62, 982
BsmFI GGGAC 2 cut(s) 639, 1040
BsnI GGCC 2 cut(s) 873, 1043
Bsp1286I GDGCHC 2 cut(s) 714, 901
Bsp143I GATC 9 cut(s) 78, 153, 457, 652, 843, 907, 1009, 1073, 1110
BspACI CCGC 2 cut(s) 84, 425
BspANI GGCC 2 cut(s) 873, 1043
BspCNI CTCAG 4 cut(s) 130, 950, 992, 1030
BspDI ATCGAT 1 cut(s) 196
BspLI GGNNCC 3 cut(s) 801, 872, 1044
BspMI ACCTGC 1 cut(s) 770
BspPI GGATC 3 cut(s) 647, 838, 1004
BspT107I GGYRCC 1 cut(s) 799
BsrDI GCAATG 2 cut(s) 111, 833
BsrFI RCCGGY 1 cut(s) 643
BsrI ACTGG 1 cut(s) 633
BssAI RCCGGY 1 cut(s) 643
BssECI CCNNGG 2 cut(s) 449, 571
BssMI GATC 9 cut(s) 78, 153, 457, 652, 843, 907, 1009, 1073, 1110
BssT1I CCWWGG 2 cut(s) 449, 571
Bst2UI CCWGG 1 cut(s) 875
Bst4CI ACNGT 1 cut(s) 720
Bst6I CTCTTC 1 cut(s) 615
BstAPI GCANNNNNTGC 1 cut(s) 113
BstBAI YACGTR 2 cut(s) 472, 692
BstC8I GCNNGC 3 cut(s) 320, 324, 645
BstDEI CTNAG 5 cut(s) 117, 312, 937, 979, 1017
BstENI CCTNNNNNAGG 1 cut(s) 777
BstF5I GGATG 6 cut(s) 107, 520, 568, 676, 883, 1092
BstH2I RGCGCY 1 cut(s) 266
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 9 cut(s) 81, 156, 460, 655, 846, 910, 1012, 1076, 1113
BstMAI GTCTC 2 cut(s) 62, 982
BstMBI GATC 9 cut(s) 78, 153, 457, 652, 843, 907, 1009, 1073, 1110
BstMWI GCNNNNNNNGC 2 cut(s) 113, 1049
BstNI CCWGG 1 cut(s) 875
BstNSI RCATGY 1 cut(s) 189
BstSCI CCNGG 1 cut(s) 873
BstSLI GKGCMC 2 cut(s) 714, 901
BstV1I GCAGC 2 cut(s) 252, 895
BstV2I GAAGAC 1 cut(s) 953
BstX2I RGATCY 3 cut(s) 1009, 1073, 1110
BstYI RGATCY 3 cut(s) 1009, 1073, 1110
Bsu15I ATCGAT 1 cut(s) 196
BsuRI GGCC 2 cut(s) 873, 1043
BsuTUI ATCGAT 1 cut(s) 196
BtsCI GGATG 6 cut(s) 107, 520, 568, 676, 883, 1092
BtsI GCAGTG 2 cut(s) 684, 745
BtsIMutI CAGTG 2 cut(s) 684, 745
BveI ACCTGC 1 cut(s) 770
Cac8I GCNNGC 3 cut(s) 320, 324, 645
CfoI GCGC 1 cut(s) 265
Cfr10I RCCGGY 1 cut(s) 643
Cfr13I GGNCC 4 cut(s) 568, 859, 871, 1042
ClaI ATCGAT 1 cut(s) 196
Csp6I GTAC 2 cut(s) 541, 838
CviAII CATG 5 cut(s) 186, 461, 841, 863, 867
CviJI RGCY 7 cut(s) 89, 318, 643, 762, 873, 886, 1043
CviKI_1 RGCY 7 cut(s) 89, 318, 643, 762, 873, 886, 1043
CviQI GTAC 2 cut(s) 541, 838
DdeI CTNAG 5 cut(s) 117, 312, 937, 979, 1017
DpnI GATC 9 cut(s) 80, 155, 459, 654, 845, 909, 1011, 1075, 1112
DpnII GATC 9 cut(s) 78, 153, 457, 652, 843, 907, 1009, 1073, 1110
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
Eam1104I CTCTTC 1 cut(s) 615
EarI CTCTTC 1 cut(s) 615
Eco130I CCWWGG 2 cut(s) 449, 571
Eco32I GATATC 1 cut(s) 602
Eco47I GGWCC 2 cut(s) 568, 859
Eco47III AGCGCT 1 cut(s) 264
Eco57I CTGAAG 1 cut(s) 192
Eco72I CACGTG 2 cut(s) 472, 692
EcoNI CCTNNNNNAGG 1 cut(s) 777
EcoO109I RGGNCCY 2 cut(s) 568, 871
EcoRI GAATTC 1 cut(s) 700
EcoRII CCWGG 1 cut(s) 873
EcoRV GATATC 1 cut(s) 602
EcoT14I CCWWGG 2 cut(s) 449, 571
EcoT22I ATGCAT 1 cut(s) 868
ErhI CCWWGG 2 cut(s) 449, 571
FaeI CATG 5 cut(s) 189, 464, 844, 866, 870
FalI AAGNNNNNCTT 2 cut(s) 805, 837
FaqI GGGAC 2 cut(s) 639, 1040
FatI CATG 5 cut(s) 185, 460, 840, 862, 866
FauI CCCGC 1 cut(s) 77
FbaI TGATCA 1 cut(s) 457
Fnu4HI GCNGC 2 cut(s) 266, 884
FokI GGATG 6 cut(s) 94, 507, 575, 663, 890, 1099
Fsp4HI GCNGC 2 cut(s) 266, 884
GlaI GCGC 1 cut(s) 264
GluI GCNGC 2 cut(s) 266, 884
HaeII RGCGCY 1 cut(s) 266
HaeIII GGCC 2 cut(s) 873, 1043
HapII CCGG 1 cut(s) 644
HhaI GCGC 1 cut(s) 265
Hin1II CATG 5 cut(s) 189, 464, 844, 866, 870
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HincII GTYRAC 2 cut(s) 934, 1162
HindII GTYRAC 2 cut(s) 934, 1162
HinfI GANTC 4 cut(s) 193, 484, 935, 1028
HpaII CCGG 1 cut(s) 644
HphI GGTGA 1 cut(s) 1006
Hpy166II GTNNAC 5 cut(s) 361, 712, 899, 934, 1162
Hpy188I TCNGA 8 cut(s) 28, 253, 538, 667, 940, 982, 1020, 1057
Hpy188III TCNNGA 6 cut(s) 119, 524, 617, 656, 813, 847
Hpy8I GTNNAC 5 cut(s) 361, 712, 899, 934, 1162
HpyAV CCTTC 4 cut(s) 216, 318, 738, 1023
HpyCH4III ACNGT 1 cut(s) 720
HpyCH4IV ACGT 4 cut(s) 471, 511, 691, 921
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 1049
HpyF3I CTNAG 5 cut(s) 117, 312, 937, 979, 1017
HpySE526I ACGT 4 cut(s) 471, 511, 691, 921
Hsp92II CATG 5 cut(s) 189, 464, 844, 866, 870
HspAI GCGC 1 cut(s) 263
KroI GCCGGC 1 cut(s) 643
KroNI GCCGGC 1 cut(s) 645
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 9 cut(s) 78, 153, 457, 652, 843, 907, 1009, 1073, 1110
LmnI GCTCC 1 cut(s) 772
Lsp1109I GCAGC 2 cut(s) 252, 895
LweI GCATC 5 cut(s) 116, 188, 529, 585, 1077
MaeII ACGT 4 cut(s) 471, 511, 691, 921
MaeIII GTNAC 2 cut(s) 749, 994
MalI GATC 9 cut(s) 80, 155, 459, 654, 845, 909, 1011, 1075, 1112
MboI GATC 9 cut(s) 78, 153, 457, 652, 843, 907, 1009, 1073, 1110
MboII GAAGA 4 cut(s) 63, 632, 709, 953
MfeI CAATTG 1 cut(s) 327
MflI RGATCY 3 cut(s) 1009, 1073, 1110
MhlI GDGCHC 2 cut(s) 714, 901
MluCI AATT 8 cut(s) 134, 234, 327, 366, 583, 612, 700, 1000
MlyI GAGTC 2 cut(s) 929, 1022
MmeI TCCRAC 1 cut(s) 316
MnlI CCTC 9 cut(s) 25, 211, 432, 559, 616, 661, 780, 1063, 1138
Mph1103I ATGCAT 1 cut(s) 868
MroNI GCCGGC 1 cut(s) 643
MseI TTAA 3 cut(s) 6, 137, 1116
MslI CAYNNNNRTG 2 cut(s) 417, 470
MspI CCGG 1 cut(s) 644
MspR9I CCNGG 1 cut(s) 875
MunI CAATTG 1 cut(s) 327
MvaI CCWGG 1 cut(s) 875
MwoI GCNNNNNNNGC 2 cut(s) 113, 1049
NaeI GCCGGC 1 cut(s) 645
NdeII GATC 9 cut(s) 78, 153, 457, 652, 843, 907, 1009, 1073, 1110
NgoMIV GCCGGC 1 cut(s) 643
NlaIII CATG 5 cut(s) 189, 464, 844, 866, 870
NlaIV GGNNCC 3 cut(s) 801, 872, 1044
NmuCI GTSAC 2 cut(s) 749, 994
NsiI ATGCAT 1 cut(s) 868
NspI RCATGY 1 cut(s) 189
PdiI GCCGGC 1 cut(s) 645
PfeI GAWTC 2 cut(s) 193, 484
PkrI GCNGC 2 cut(s) 267, 885
PleI GAGTC 2 cut(s) 929, 1022
PmaCI CACGTG 2 cut(s) 472, 692
PmlI CACGTG 2 cut(s) 472, 692
PpsI GAGTC 2 cut(s) 929, 1022
Ppu21I YACGTR 2 cut(s) 472, 692
PpuMI RGGWCCY 1 cut(s) 568
PshBI ATTAAT 1 cut(s) 6
PsiI TTATAA 1 cut(s) 726
Psp1406I AACGTT 2 cut(s) 511, 921
Psp5II RGGWCCY 1 cut(s) 568
Psp6I CCWGG 1 cut(s) 873
PspCI CACGTG 2 cut(s) 472, 692
PspGI CCWGG 1 cut(s) 873
PspN4I GGNNCC 3 cut(s) 801, 872, 1044
PspPI GGNCC 4 cut(s) 568, 859, 871, 1042
PspPPI RGGWCCY 1 cut(s) 568
PsuI RGATCY 3 cut(s) 1009, 1073, 1110
RsaI GTAC 2 cut(s) 542, 839
RsaNI GTAC 2 cut(s) 541, 838
RseI CAYNNNNRTG 2 cut(s) 417, 470
SaqAI TTAA 3 cut(s) 6, 137, 1116
SatI GCNGC 2 cut(s) 266, 884
Sau3AI GATC 9 cut(s) 78, 153, 457, 652, 843, 907, 1009, 1073, 1110
Sau96I GGNCC 4 cut(s) 568, 859, 871, 1042
SchI GAGTC 2 cut(s) 929, 1022
ScrFI CCNGG 1 cut(s) 875
SduI GDGCHC 2 cut(s) 714, 901
SfaNI GCATC 5 cut(s) 116, 188, 529, 585, 1077
SinI GGWCC 2 cut(s) 568, 859
SmiMI CAYNNNNRTG 2 cut(s) 417, 470
Sse9I AATT 8 cut(s) 134, 234, 327, 366, 583, 612, 700, 1000
SsiI CCGC 2 cut(s) 84, 425
StyD4I CCNGG 1 cut(s) 873
StyI CCWWGG 2 cut(s) 449, 571
TaaI ACNGT 1 cut(s) 720
TaiI ACGT 4 cut(s) 474, 514, 694, 924
TaqI TCGA 3 cut(s) 196, 910, 1031
TasI AATT 8 cut(s) 134, 234, 327, 366, 583, 612, 700, 1000
TatI WGTACW 1 cut(s) 540
TfiI GAWTC 2 cut(s) 193, 484
Tru1I TTAA 3 cut(s) 6, 137, 1116
Tru9I TTAA 3 cut(s) 6, 137, 1116
TscAI CASTG 2 cut(s) 691, 745
TseFI GTSAC 2 cut(s) 749, 994
TseI GCWGC 2 cut(s) 265, 883
Tsp45I GTSAC 2 cut(s) 749, 994
TspDTI ATGAA 7 cut(s) 63, 85, 270, 309, 401, 497, 941
TspRI CASTG 2 cut(s) 691, 745
VneI GTGCAC 2 cut(s) 710, 897
VpaK11BI GGWCC 2 cut(s) 568, 859
VspI ATTAAT 1 cut(s) 6
XagI CCTNNNNNAGG 1 cut(s) 777
XapI RAATTY 2 cut(s) 583, 700
XceI RCATGY 1 cut(s) 189
Zsp2I ATGCAT 1 cut(s) 868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.