AT4G20990

Alpha carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012107
Physical Location & Seq
Forward (+)
11219772 .. 11221148
1377 bp
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UTR
Exon/CDS
Intron
AT4G20990.1

Sequence Viewer

Length: 804 bp
ATGGATACCAACGCAAAAACAATTTTCTTCATGGCTATGTGTTTCATCTATCTATCTTTCCCTAATATTTCACACGCTCATTCTGAAGTCGACGACGAAACTCCATTTACTTACGAACAAAAAACGGAAAAGGGACCAGAGGGATGGGGCAAAATAAATCCGCACTGGAAAGTTTGTAACACCGGAAGATATCAATCCCCGATCGATCTTACTAACGAAAGAGTCAGTCTTATTCATGATCAAGCATGGACAAGACAATATAAACCAGCTCCGGCTGTAATTACAAACAGAGGCCATGACATTATGGTATCATGGAAAGGAGATGCTGGGAAGATGACAATACGGAAAACGGATTTTAATTTGGTGCAATGCCATTGGCATTCACCTTCTGAGCATACCGTTAACGGAACTAGGTACGACCTAGAGCTTCACATGGTTCACACGAGTGCACGAGGCAGAACTGCGGTTATCGGAGTTCTTTACAAATTAGGCGAACCTAATGAATTCCTCACCAAGCTACTAAATGGAATAAAAGCAGTGGGAAATAAAGAGATAAATCTAGGGATGATTGATCCACGAGAGATTAGGTTTCAAACAAGAAAATTCTATAGATACATTGGCTCTCTCACTGTTCCTCCTTGCACTGAAGGCGTCATTTGGACTGTCGTCAAAAGGGTGAACACAATATCAATGGAGCAAATTACAGCTCTTAGGCAAGCCGTTGACGATGGATTTGAGACAAATTCAAGACCGGTTCAAGACTCAAAGGGAAGATCAGTTTGGTTCTATGATCCAAATGTTTGA

Protein Analysis

267

Amino Acids

30.59

Weight (kDa)

8.59

Isoelectric Point (pI)

31.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 45 - 261 1e-39 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012107)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20990
fragaria_vesca FvH4_6g24590 FvH4_6g24590 FvH4_6g24590
malus_domestica MD12G1083000.v1.1 MD14G1078100.v1.1
prunus_persica Prupe.7G052800_v2.0.a1
pyrus_communis pycom12g06990 pycom14g06650
rosa_chinensis RchiOBHm_Chr3g0483901
rosa_laevigata RLG00000023253
rosa_multiflora Rmu_sc0002252.1_g000013
rosa_roxburghii Rroxscaffold_6G00398060
rosa_rugosa Rorug03G0208600
rosa_samantha Rh3AG257300 Rh3BG293700 Rh3CG292000 Rh3DG287100
rosa_wichuraiana Rw3G023260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 90
AciI CCGC 2 cut(s) 161, 464
AclWI GGATC 2 cut(s) 566, 785
AcsI RAATTY 3 cut(s) 503, 602, 742
AcuI CTGAAG 2 cut(s) 105, 666
AcyI GRCGYC 1 cut(s) 651
AfaI GTAC 1 cut(s) 416
AgeI ACCGGT 1 cut(s) 751
AgsI TTSAA 3 cut(s) 593, 747, 758
AjuI GAANNNNNNNTTGG 2 cut(s) 763, 795
AleI CACNNNNGTG 1 cut(s) 444
AluBI AGCT 4 cut(s) 269, 427, 517, 707
AluI AGCT 4 cut(s) 269, 427, 517, 707
Alw21I GWGCWC 1 cut(s) 451
Alw26I GTCTC 1 cut(s) 731
Alw44I GTGCAC 1 cut(s) 447
AlwI GGATC 2 cut(s) 566, 785
AoxI GGCC 1 cut(s) 292
ApaLI GTGCAC 1 cut(s) 447
ApoI RAATTY 3 cut(s) 503, 602, 742
ArsI GACNNNNNNTTYG 2 cut(s) 716, 748
AsiGI ACCGGT 1 cut(s) 751
AspS9I GGNCC 1 cut(s) 134
AsuHPI GGTGA 3 cut(s) 375, 502, 688
AvaII GGWCC 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 451
BauI CACGAG 3 cut(s) 442, 450, 576
Bbv12I GWGCWC 1 cut(s) 451
BccI CCATC 2 cut(s) 138, 722
BceAI ACGGC 1 cut(s) 704
BclI TGATCA 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 731
BfaI CTAG 3 cut(s) 411, 422, 560
BfmI CTRYAG 1 cut(s) 607
Bme18I GGWCC 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 134
BmiI GGNNCC 1 cut(s) 135
BmsI GCATC 1 cut(s) 313
BoxI GACNNNNGTC 1 cut(s) 665
Bsa29I ATCGAT 1 cut(s) 204
BsaBI GATNNNNATC 1 cut(s) 193
BsaHI GRCGYC 1 cut(s) 651
BsaWI WCCGGW 2 cut(s) 182, 751
BsaXI ACNNNNNCTCC 2 cut(s) 619, 649
Bse118I RCCGGY 1 cut(s) 751
Bse1I ACTGG 1 cut(s) 170
Bse3DI GCAATG 1 cut(s) 374
Bse8I GATNNNNATC 1 cut(s) 193
BseCI ATCGAT 1 cut(s) 204
BseGI GGATG 2 cut(s) 149, 570
BseJI GATNNNNATC 1 cut(s) 193
BseMI GCAATG 1 cut(s) 374
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 1 cut(s) 170
BseSI GKGCMC 1 cut(s) 451
BseYI CCCAGC 1 cut(s) 326
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 1 cut(s) 294
BshTI ACCGGT 1 cut(s) 751
BshVI ATCGAT 1 cut(s) 204
BsiEI CGRYCG 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 451
BsiSI CCGG 3 cut(s) 183, 272, 752
BslFI GGGAC 1 cut(s) 147
BsmAI GTCTC 1 cut(s) 731
BsmFI GGGAC 1 cut(s) 147
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 451
Bsp143I GATC 6 cut(s) 201, 205, 238, 571, 773, 790
BspACI CCGC 2 cut(s) 161, 464
BspANI GGCC 1 cut(s) 294
BspCNI CTCAG 1 cut(s) 382
BspDI ATCGAT 1 cut(s) 204
BspHI TCATGA 1 cut(s) 235
BspLI GGNNCC 1 cut(s) 135
BspPI GGATC 2 cut(s) 566, 785
BsrDI GCAATG 1 cut(s) 374
BsrFI RCCGGY 1 cut(s) 751
BsrI ACTGG 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 751
BssMI GATC 6 cut(s) 201, 205, 238, 571, 773, 790
BssNI GRCGYC 1 cut(s) 651
BssSI CACGAG 3 cut(s) 442, 450, 576
Bst2BI CACGAG 3 cut(s) 442, 450, 576
Bst4CI ACNGT 3 cut(s) 400, 631, 664
BstACI GRCGYC 1 cut(s) 651
BstC8I GCNNGC 1 cut(s) 717
BstDEI CTNAG 2 cut(s) 390, 710
BstF5I GGATG 2 cut(s) 149, 570
BstKTI GATC 6 cut(s) 204, 208, 241, 574, 776, 793
BstMAI GTCTC 1 cut(s) 731
BstMBI GATC 6 cut(s) 201, 205, 238, 571, 773, 790
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 1 cut(s) 648
BstPAI GACNNNNGTC 1 cut(s) 665
BstSFI CTRYAG 1 cut(s) 607
BstSLI GKGCMC 1 cut(s) 451
BstXI CCANNNNNNTGG 1 cut(s) 144
Bsu15I ATCGAT 1 cut(s) 204
BsuRI GGCC 1 cut(s) 294
BsuTUI ATCGAT 1 cut(s) 204
BtsCI GGATG 2 cut(s) 149, 570
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 4 cut(s) 163, 543, 627, 642
Cac8I GCNNGC 1 cut(s) 717
CciI TCATGA 1 cut(s) 235
Cfr10I RCCGGY 1 cut(s) 751
Cfr13I GGNCC 1 cut(s) 134
ClaI ATCGAT 1 cut(s) 204
CseI GACGC 1 cut(s) 640
Csp6I GTAC 1 cut(s) 415
CspAI ACCGGT 1 cut(s) 751
CviAII CATG 6 cut(s) 31, 236, 246, 296, 312, 433
CviJI RGCY 9 cut(s) 35, 269, 275, 294, 427, 517, 621, 707, 719
CviKI_1 RGCY 9 cut(s) 35, 269, 275, 294, 427, 517, 621, 707, 719
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 2 cut(s) 390, 710
DpnI GATC 6 cut(s) 203, 207, 240, 573, 775, 792
DpnII GATC 6 cut(s) 201, 205, 238, 571, 773, 790
Eco32I GATATC 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 134
Eco57I CTGAAG 2 cut(s) 105, 666
EcoRI GAATTC 1 cut(s) 503
EcoRV GATATC 1 cut(s) 191
FaeI CATG 6 cut(s) 34, 239, 249, 299, 315, 436
FaqI GGGAC 1 cut(s) 147
FatI CATG 6 cut(s) 30, 235, 245, 295, 311, 432
FbaI TGATCA 1 cut(s) 238
FblI GTMKAC 1 cut(s) 90
FokI GGATG 2 cut(s) 156, 577
FspBI CTAG 3 cut(s) 411, 422, 560
GsaI CCCAGC 1 cut(s) 330
HaeIII GGCC 1 cut(s) 294
HapII CCGG 3 cut(s) 183, 272, 752
HgaI GACGC 1 cut(s) 640
Hin1I GRCGYC 1 cut(s) 651
Hin1II CATG 6 cut(s) 34, 239, 249, 299, 315, 436
HincII GTYRAC 3 cut(s) 91, 403, 724
HindII GTYRAC 3 cut(s) 91, 403, 724
HinfI GANTC 2 cut(s) 222, 761
HpaI GTTAAC 1 cut(s) 403
HpaII CCGG 3 cut(s) 183, 272, 752
HphI GGTGA 3 cut(s) 375, 502, 688
Hpy166II GTNNAC 6 cut(s) 91, 403, 439, 449, 679, 724
Hpy188I TCNGA 3 cut(s) 85, 391, 473
Hpy188III TCNNGA 3 cut(s) 236, 747, 758
Hpy8I GTNNAC 6 cut(s) 91, 403, 439, 449, 679, 724
Hpy99I CGWCG 2 cut(s) 95, 98
HpyAV CCTTC 2 cut(s) 396, 641
HpyCH4III ACNGT 3 cut(s) 400, 631, 664
HpyCH4V TGCA 3 cut(s) 367, 449, 642
HpyF10VI GCNNNNNNNGC 1 cut(s) 648
HpyF3I CTNAG 2 cut(s) 390, 710
Hsp92I GRCGYC 1 cut(s) 651
Hsp92II CATG 6 cut(s) 34, 239, 249, 299, 315, 436
Ksp22I TGATCA 1 cut(s) 238
KspAI GTTAAC 1 cut(s) 403
Kzo9I GATC 6 cut(s) 201, 205, 238, 571, 773, 790
LmnI GCTCC 2 cut(s) 274, 694
LpnPI CCDG 7 cut(s) 150, 151, 196, 279, 285, 312, 765
LweI GCATC 1 cut(s) 313
MaeI CTAG 3 cut(s) 411, 422, 560
MaeIII GTNAC 1 cut(s) 176
MalI GATC 6 cut(s) 203, 207, 240, 573, 775, 792
MboI GATC 6 cut(s) 201, 205, 238, 571, 773, 790
MboII GAAGA 4 cut(s) 19, 198, 343, 783
MhlI GDGCHC 1 cut(s) 451
MluCI AATT 8 cut(s) 21, 279, 358, 485, 503, 602, 699, 742
MlyI GAGTC 2 cut(s) 231, 755
MnlI CCTC 5 cut(s) 133, 284, 446, 518, 645
MseI TTAA 2 cut(s) 357, 402
MslI CAYNNNNRTG 2 cut(s) 35, 444
MspI CCGG 3 cut(s) 183, 272, 752
Mva1269I GAATGC 1 cut(s) 379
MwoI GCNNNNNNNGC 1 cut(s) 648
NdeII GATC 6 cut(s) 201, 205, 238, 571, 773, 790
NlaIII CATG 6 cut(s) 34, 239, 249, 299, 315, 436
NlaIV GGNNCC 1 cut(s) 135
OliI CACNNNNGTG 1 cut(s) 444
PagI TCATGA 1 cut(s) 235
PctI GAATGC 1 cut(s) 379
PinAI ACCGGT 1 cut(s) 751
Ple19I CGATCG 1 cut(s) 204
PleI GAGTC 2 cut(s) 230, 755
PpsI GAGTC 2 cut(s) 230, 755
PshAI GACNNNNGTC 1 cut(s) 665
PspFI CCCAGC 1 cut(s) 326
PspN4I GGNNCC 1 cut(s) 135
PspPI GGNCC 1 cut(s) 134
PvuI CGATCG 1 cut(s) 204
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
RseI CAYNNNNRTG 2 cut(s) 35, 444
SalI GTCGAC 1 cut(s) 89
SaqAI TTAA 2 cut(s) 357, 402
Sau3AI GATC 6 cut(s) 201, 205, 238, 571, 773, 790
Sau96I GGNCC 1 cut(s) 134
SchI GAGTC 2 cut(s) 231, 755
SduI GDGCHC 1 cut(s) 451
SetI ASST 9 cut(s) 271, 388, 416, 423, 429, 499, 519, 590, 709
SfaNI GCATC 1 cut(s) 313
SfcI CTRYAG 1 cut(s) 607
SinI GGWCC 1 cut(s) 134
SmiMI CAYNNNNRTG 2 cut(s) 35, 444
Sse9I AATT 8 cut(s) 21, 279, 358, 485, 503, 602, 699, 742
SsiI CCGC 2 cut(s) 161, 464
SspI AATATT 1 cut(s) 67
SspMI CTAG 3 cut(s) 411, 422, 560
TaaI ACNGT 3 cut(s) 400, 631, 664
TaqI TCGA 2 cut(s) 90, 204
TasI AATT 8 cut(s) 21, 279, 358, 485, 503, 602, 699, 742
Tru1I TTAA 2 cut(s) 357, 402
Tru9I TTAA 2 cut(s) 357, 402
TscAI CASTG 4 cut(s) 170, 543, 634, 649
TspDTI ATGAA 4 cut(s) 19, 34, 224, 516
TspGWI ACGGA 4 cut(s) 140, 358, 365, 420
TspRI CASTG 4 cut(s) 170, 543, 634, 649
VneI GTGCAC 1 cut(s) 447
VpaK11BI GGWCC 1 cut(s) 134
XapI RAATTY 3 cut(s) 503, 602, 742
XmiI GTMKAC 1 cut(s) 90
XspI CTAG 3 cut(s) 411, 422, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.