MD14G1078100.v1.1

carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012107
Physical Location & Seq
Reverse (-)
8924662 .. 8929000
4339 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1078100.v1.1.491

Sequence Viewer

Length: 843 bp
ATGACCAAACCCATCTTCTTCTTCTTTCTCTTTCTAGCTTCTCTCATCTTTTCGTCCCATCACTCCTTTCTCACCATTTGCAATGCAGCACTTGAGGACTCTGATGAAGTTGGTGATGAAACTGCATTTACTTATCTTGAAGGAACTGGTAAAGGACCAAAGAAATGGGGCCAAATTGACCCACAATGGAAAGTTTGTGACAACGGAAAAATGCAATCTCCAATTGATCTTCTTGACCAAAGGGTGCAGGTTTTCCCTAAATTGGGGAAACTGAAAAGGGAATACAAACCGGCTCCTGCTATTGTGAAGAACCGAGGTCATGACATCACGATGAGGTGGAATGGAGATGCAGGAAAAACCAAGATAAATGGGACTGACTATAGACTGCTACAATGTCATTGGCATTCACCCTCCGAACACACATTTAACGGATCAAGGTATGCTTTGGAGTTTCATATCGTTCATGTAAGCTCTACCGGAAAGATTGCTGTTATTGGAATTGTGTACAAATATGGTCGACCTGATCCCTTCCTTTCAAAGCTGTTCCACCACATAAAATCAGTTGGAAAAGAAGAGGTTGACATAGGGATTATTAATCCTGGAGATATTAAGTTTGGAAGCAGAAAGTACTACAGATATATCGGTTCTCTTACCGTTCCTCCATGCACTGAGGGTGTAATTTGGACAATCGTCAAAAAGGTCAGGACAGTTTCAAGGGAGCAAGTGCGTGCACTACGGGAGGCGGTTCATGATGGATATGAGGCGAATGCAAGGCCAACTCAAGAACTGGATGGAAGACCAGTGTTGTTATACACTCCAAGGAATAATGGAGGTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase LSM

Protein Analysis

281

Amino Acids

31.62

Weight (kDa)

9.29

Isoelectric Point (pI)

28.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 52 - 269 3.9e-41 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012107)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20990
fragaria_vesca FvH4_6g24590 FvH4_6g24590 FvH4_6g24590
malus_domestica MD12G1083000.v1.1 MD14G1078100.v1.1
prunus_persica Prupe.7G052800_v2.0.a1
pyrus_communis pycom12g06990 pycom14g06650
rosa_chinensis RchiOBHm_Chr3g0483901
rosa_laevigata RLG00000023253
rosa_multiflora Rmu_sc0002252.1_g000013
rosa_roxburghii Rroxscaffold_6G00398060
rosa_rugosa Rorug03G0208600
rosa_samantha Rh3AG257300 Rh3BG293700 Rh3CG292000 Rh3DG287100
rosa_wichuraiana Rw3G023260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 238
AccI GTMKAC 1 cut(s) 517
AciI CCGC 1 cut(s) 743
AclWI GGATC 2 cut(s) 439, 518
AfaI GTAC 2 cut(s) 506, 629
AfiI CCNNNNNNNGG 2 cut(s) 262, 263
AgsI TTSAA 3 cut(s) 140, 537, 714
AjnI CCWGG 1 cut(s) 598
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AluBI AGCT 3 cut(s) 38, 471, 541
AluI AGCT 3 cut(s) 38, 471, 541
Alw21I GWGCWC 1 cut(s) 733
Alw44I GTGCAC 1 cut(s) 729
AlwI GGATC 2 cut(s) 439, 518
AoxI GGCC 2 cut(s) 169, 773
ApaLI GTGCAC 1 cut(s) 729
ApeKI GCWGC 1 cut(s) 86
AseI ATTAAT 1 cut(s) 594
AspS9I GGNCC 2 cut(s) 155, 169
AsuHPI GGTGA 3 cut(s) 64, 125, 399
AvaII GGWCC 1 cut(s) 155
BaeGI GKGCMC 1 cut(s) 733
BbsI GAAGAC 1 cut(s) 802
Bbv12I GWGCWC 1 cut(s) 733
BbvI GCAGC 1 cut(s) 98
BccI CCATC 4 cut(s) 20, 66, 746, 785
BciT130I CCWGG 1 cut(s) 600
BfaI CTAG 1 cut(s) 35
BfmI CTRYAG 2 cut(s) 379, 631
BfuAI ACCTGC 1 cut(s) 238
BisI GCNGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 88
BmcAI AGTACT 1 cut(s) 629
Bme1390I CCNGG 1 cut(s) 600
Bme18I GGWCC 1 cut(s) 155
BmgT120I GGNCC 2 cut(s) 155, 169
BmiI GGNNCC 2 cut(s) 170, 294
BmrFI CCNGG 1 cut(s) 600
BmsI GCATC 1 cut(s) 337
BoxI GACNNNNGTC 1 cut(s) 689
BpiI GAAGAC 1 cut(s) 802
BpmI CTGGAG 1 cut(s) 621
BpuEI CTTGAG 2 cut(s) 113, 765
BsaJI CCNNGG 2 cut(s) 313, 818
BsaWI WCCGGW 1 cut(s) 476
BsaXI ACNNNNNCTCC 2 cut(s) 643, 673
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 263
Bse118I RCCGGY 1 cut(s) 289
Bse1I ACTGG 3 cut(s) 151, 792, 800
Bse3DI GCAATG 1 cut(s) 88
BseBI CCWGG 1 cut(s) 600
BseDI CCNNGG 2 cut(s) 313, 818
BseGI GGATG 1 cut(s) 796
BseLI CCNNNNNNNGG 2 cut(s) 262, 263
BseMI GCAATG 1 cut(s) 88
BseMII CTCAG 1 cut(s) 660
BseNI ACTGG 3 cut(s) 151, 792, 800
BseSI GKGCMC 1 cut(s) 733
BseXI GCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 266
BshFI GGCC 2 cut(s) 171, 775
BsiHKAI GWGCWC 1 cut(s) 733
BsiSI CCGG 2 cut(s) 290, 477
BslFI GGGAC 2 cut(s) 40, 385
BslI CCNNNNNNNGG 2 cut(s) 262, 263
BsmFI GGGAC 2 cut(s) 40, 385
BsmI GAATGC 2 cut(s) 403, 772
BsnI GGCC 2 cut(s) 171, 775
Bsp1286I GDGCHC 1 cut(s) 733
Bsp1407I TGTACA 1 cut(s) 504
Bsp143I GATC 3 cut(s) 226, 431, 523
BspACI CCGC 1 cut(s) 743
BspANI GGCC 2 cut(s) 171, 775
BspCNI CTCAG 1 cut(s) 661
BspHI TCATGA 2 cut(s) 319, 748
BspLI GGNNCC 2 cut(s) 170, 294
BspMI ACCTGC 1 cut(s) 238
BspPI GGATC 2 cut(s) 439, 518
BsrDI GCAATG 1 cut(s) 88
BsrFI RCCGGY 1 cut(s) 289
BsrGI TGTACA 1 cut(s) 504
BsrI ACTGG 3 cut(s) 151, 792, 800
BssAI RCCGGY 1 cut(s) 289
BssECI CCNNGG 2 cut(s) 313, 818
BssMI GATC 3 cut(s) 226, 431, 523
BssT1I CCWWGG 1 cut(s) 818
Bst2UI CCWGG 1 cut(s) 600
Bst4CI ACNGT 2 cut(s) 655, 709
Bst6I CTCTTC 1 cut(s) 567
BstAUI TGTACA 1 cut(s) 504
BstC8I GCNNGC 1 cut(s) 729
BstDEI CTNAG 1 cut(s) 669
BstF5I GGATG 1 cut(s) 796
BstKTI GATC 3 cut(s) 229, 434, 526
BstMBI GATC 3 cut(s) 226, 431, 523
BstNI CCWGG 1 cut(s) 600
BstPAI GACNNNNGTC 1 cut(s) 689
BstSCI CCNGG 1 cut(s) 598
BstSFI CTRYAG 2 cut(s) 379, 631
BstSLI GKGCMC 1 cut(s) 733
BstV1I GCAGC 1 cut(s) 98
BstV2I GAAGAC 1 cut(s) 802
BstXI CCANNNNNNTGG 1 cut(s) 165
BsuRI GGCC 2 cut(s) 171, 775
BtsCI GGATG 1 cut(s) 796
BtsIMutI CAGTG 2 cut(s) 666, 807
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 1 cut(s) 729
CciI TCATGA 2 cut(s) 319, 748
Cfr10I RCCGGY 1 cut(s) 289
Cfr13I GGNCC 2 cut(s) 155, 169
Csp6I GTAC 2 cut(s) 505, 628
CviAII CATG 4 cut(s) 320, 464, 663, 749
CviJI RGCY 6 cut(s) 38, 171, 293, 471, 541, 775
CviKI_1 RGCY 6 cut(s) 38, 171, 293, 471, 541, 775
CviQI GTAC 2 cut(s) 505, 628
DdeI CTNAG 1 cut(s) 669
DpnI GATC 3 cut(s) 228, 433, 525
DpnII GATC 3 cut(s) 226, 431, 523
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
Eco130I CCWWGG 1 cut(s) 818
Eco47I GGWCC 1 cut(s) 155
EcoRII CCWGG 1 cut(s) 598
EcoT14I CCWWGG 1 cut(s) 818
ErhI CCWWGG 1 cut(s) 818
FaeI CATG 4 cut(s) 323, 467, 666, 752
FalI AAGNNNNNCTT 2 cut(s) 427, 459
FaqI GGGAC 2 cut(s) 40, 385
FatI CATG 4 cut(s) 319, 463, 662, 748
FblI GTMKAC 1 cut(s) 517
Fnu4HI GCNGC 1 cut(s) 87
FokI GGATG 1 cut(s) 803
Fsp4HI GCNGC 1 cut(s) 87
FspBI CTAG 1 cut(s) 35
GluI GCNGC 1 cut(s) 87
GsuI CTGGAG 1 cut(s) 621
HaeIII GGCC 2 cut(s) 171, 775
HapII CCGG 2 cut(s) 290, 477
Hin1II CATG 4 cut(s) 323, 467, 666, 752
HincII GTYRAC 2 cut(s) 518, 580
HindII GTYRAC 2 cut(s) 518, 580
HinfI GANTC 1 cut(s) 98
HpaII CCGG 2 cut(s) 290, 477
HphI GGTGA 3 cut(s) 64, 125, 399
Hpy166II GTNNAC 4 cut(s) 505, 518, 580, 731
Hpy188I TCNGA 2 cut(s) 103, 415
Hpy188III TCNNGA 7 cut(s) 137, 233, 320, 328, 703, 749, 782
Hpy8I GTNNAC 4 cut(s) 505, 518, 580, 731
HpyAV CCTTC 2 cut(s) 134, 538
HpyCH4III ACNGT 2 cut(s) 655, 709
HpyCH4V TGCA 9 cut(s) 81, 86, 125, 214, 247, 350, 666, 731, 770
HpyF3I CTNAG 1 cut(s) 669
Hsp92II CATG 4 cut(s) 323, 467, 666, 752
Kzo9I GATC 3 cut(s) 226, 431, 523
LmnI GCTCC 2 cut(s) 298, 718
Lsp1109I GCAGC 1 cut(s) 98
LweI GCATC 1 cut(s) 337
MaeI CTAG 1 cut(s) 35
MaeIII GTNAC 1 cut(s) 197
MalI GATC 3 cut(s) 228, 433, 525
MboI GATC 3 cut(s) 226, 431, 523
MboII GAAGA 7 cut(s) 7, 10, 13, 221, 319, 584, 807
MfeI CAATTG 1 cut(s) 222
MhlI GDGCHC 1 cut(s) 733
MluCI AATT 5 cut(s) 174, 222, 260, 498, 678
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 544
MseI TTAA 4 cut(s) 426, 594, 609, 841
MslI CAYNNNNRTG 1 cut(s) 329
MspI CCGG 2 cut(s) 290, 477
MspR9I CCNGG 1 cut(s) 600
MunI CAATTG 1 cut(s) 222
Mva1269I GAATGC 2 cut(s) 403, 772
MvaI CCWGG 1 cut(s) 600
NdeII GATC 3 cut(s) 226, 431, 523
NlaIII CATG 4 cut(s) 323, 467, 666, 752
NlaIV GGNNCC 2 cut(s) 170, 294
NmuCI GTSAC 1 cut(s) 197
PagI TCATGA 2 cut(s) 319, 748
PctI GAATGC 2 cut(s) 403, 772
PfoI TCCNGGA 1 cut(s) 598
PkrI GCNGC 1 cut(s) 88
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
PshAI GACNNNNGTC 1 cut(s) 689
PshBI ATTAAT 1 cut(s) 594
Psp6I CCWGG 1 cut(s) 598
PspGI CCWGG 1 cut(s) 598
PspN4I GGNNCC 2 cut(s) 170, 294
PspPI GGNCC 2 cut(s) 155, 169
RsaI GTAC 2 cut(s) 506, 629
RsaNI GTAC 2 cut(s) 505, 628
RseI CAYNNNNRTG 1 cut(s) 329
SalI GTCGAC 1 cut(s) 516
SaqAI TTAA 4 cut(s) 426, 594, 609, 841
SatI GCNGC 1 cut(s) 87
Sau3AI GATC 3 cut(s) 226, 431, 523
Sau96I GGNCC 2 cut(s) 155, 169
ScaI AGTACT 1 cut(s) 629
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 600
SduI GDGCHC 1 cut(s) 733
SfaNI GCATC 1 cut(s) 337
SfcI CTRYAG 2 cut(s) 379, 631
SinI GGWCC 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 329
SmlI CTYRAG 2 cut(s) 92, 780
SmoI CTYRAG 2 cut(s) 92, 780
Sse9I AATT 5 cut(s) 174, 222, 260, 498, 678
SsiI CCGC 1 cut(s) 743
SspMI CTAG 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 598
StyI CCWWGG 1 cut(s) 818
TaaI ACNGT 2 cut(s) 655, 709
TaqI TCGA 1 cut(s) 517
TasI AATT 5 cut(s) 174, 222, 260, 498, 678
TatI WGTACW 2 cut(s) 504, 627
Tru1I TTAA 4 cut(s) 426, 594, 609, 841
Tru9I TTAA 4 cut(s) 426, 594, 609, 841
TscAI CASTG 2 cut(s) 673, 807
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 1 cut(s) 86
Tsp45I GTSAC 1 cut(s) 197
TspDTI ATGAA 5 cut(s) 120, 132, 443, 452, 737
TspGWI ACGGA 2 cut(s) 219, 444
TspRI CASTG 2 cut(s) 673, 807
VneI GTGCAC 1 cut(s) 729
VpaK11BI GGWCC 1 cut(s) 155
VspI ATTAAT 1 cut(s) 594
XmiI GTMKAC 1 cut(s) 517
XspI CTAG 1 cut(s) 35
ZrmI AGTACT 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.