Rroxscaffold_6G00398060

carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0012107
Physical Location & Seq
Reverse (-)
19892320 .. 19896387
4068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00398060.1

Sequence Viewer

Length: 903 bp
ATGCCATTTCCAATTTTGAAGCAACGAAGCATTATGTGGTTCTTCAATCCCAATCCCAACCCCAAACCCAACTTGTTCTTCTTCTTTCTCATTTTGGGTTCCCTCCTCCTCTTATCCCATCATTCCTTTTTCACCATTTGCAGAGCCGCACTTGATGACTCCGGGGAAGTTGGTGATGAACTTCCATTTACTTATGTTGAGGGAACTGGTAAAGGACCAAAGAAATGGGGTCAAATTGACCCACATTGGAAAGTTTGTGATAATGGAGAAATGCAGTCTCCTATTGATCTTCTTGACGGCAGGGTGCAGGTTTTCCCTAACTTGGGGAAATTGAAAAGGGATTACAAACAAGCCCCTGCTACTATCAAGAACAGAGGGCATGACATTACGGTGAGGTGGAAAGAAGACGCAGGGAAAATCAATATTAATGGTACAGACTATAATCTGCTACAATGTCATTGGCATTCACCTTCTGAACACACATTCAATGGATCAAGGTATGCTTTGGAGTTGCACGTTATTCATACAAGCTCTACTGGAAAGATTGCTGTTATTGGAATTGTATACAAATATGGCCGACCTGATCCCTTCCTTTCAAAGCTGTTCCACCACATAAAATCAGTTGGGAAAGAAGAGGTTGATATTGGCATTATTAATCCAGGAGACATCAAGTTTGGAAGCAGAAAGTATTACAGATATATTGGTTCTCTCACTGTTCCTCCCTGCACTGAGGGTGTAATTTGGACAATAGTCAAAAAGGTGAGGACAGTTTCAAGGGAGCAAGTGAGAGCACTAAGGGAGACTGTTCATGATGGATATGAGGAAAATGCAAGGCCAACTCAGCAATTCAATGGGAGACCAGTGTTGTTATACACTCCAGGGGAGAATGGAGGTTCTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase LSM

Protein Analysis

300

Amino Acids

33.98

Weight (kDa)

9.12

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 72 - 289 3.4e-42 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012107)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20990
fragaria_vesca FvH4_6g24590 FvH4_6g24590 FvH4_6g24590
malus_domestica MD12G1083000.v1.1 MD14G1078100.v1.1
prunus_persica Prupe.7G052800_v2.0.a1
pyrus_communis pycom12g06990 pycom14g06650
rosa_chinensis RchiOBHm_Chr3g0483901
rosa_laevigata RLG00000023253
rosa_multiflora Rmu_sc0002252.1_g000013
rosa_roxburghii Rroxscaffold_6G00398060
rosa_rugosa Rorug03G0208600
rosa_samantha Rh3AG257300 Rh3BG293700 Rh3CG292000 Rh3DG287100
rosa_wichuraiana Rw3G023260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 298
AccI GTMKAC 1 cut(s) 564
AciI CCGC 1 cut(s) 147
AclWI GGATC 2 cut(s) 499, 578
AcoI YGGCCR 1 cut(s) 574
AfaI GTAC 1 cut(s) 433
AfiI CCNNNNNNNGG 2 cut(s) 322, 323
AgsI TTSAA 7 cut(s) 19, 46, 334, 487, 597, 774, 850
AjnI CCWGG 2 cut(s) 658, 877
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
AluBI AGCT 2 cut(s) 531, 601
AluI AGCT 2 cut(s) 531, 601
Alw21I GWGCWC 1 cut(s) 793
Alw26I GTCTC 4 cut(s) 282, 657, 794, 850
AlwI GGATC 2 cut(s) 499, 578
AoxI GGCC 2 cut(s) 574, 833
ArsI GACNNNNNNTTYG 2 cut(s) 656, 688
AseI ATTAAT 2 cut(s) 426, 654
AspS9I GGNCC 1 cut(s) 215
AsuC2I CCSGG 1 cut(s) 163
AsuHPI GGTGA 5 cut(s) 124, 185, 403, 459, 772
AvaII GGWCC 1 cut(s) 215
BbsI GAAGAC 1 cut(s) 411
Bbv12I GWGCWC 1 cut(s) 793
BccI CCATC 2 cut(s) 126, 806
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 2 cut(s) 660, 879
BcnI CCSGG 1 cut(s) 163
BcoDI GTCTC 4 cut(s) 282, 657, 794, 850
BfuAI ACCTGC 1 cut(s) 298
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
Bme1390I CCNGG 3 cut(s) 163, 660, 879
Bme18I GGWCC 1 cut(s) 215
BmgT120I GGNCC 1 cut(s) 215
BmiI GGNNCC 1 cut(s) 100
BmrFI CCNGG 3 cut(s) 163, 660, 879
BoxI GACNNNNGTC 1 cut(s) 749
BpiI GAAGAC 1 cut(s) 411
BpmI CTGGAG 1 cut(s) 861
BpuMI CCSGG 1 cut(s) 163
BsaI GGTCTC 1 cut(s) 850
BsaJI CCNNGG 2 cut(s) 162, 878
BsaXI ACNNNNNCTCC 2 cut(s) 703, 733
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 323
Bse1I ACTGG 3 cut(s) 211, 541, 860
BseBI CCWGG 2 cut(s) 660, 879
BseDI CCNNGG 2 cut(s) 162, 878
BseLI CCNNNNNNNGG 2 cut(s) 322, 323
BseMII CTCAG 2 cut(s) 720, 854
BseNI ACTGG 3 cut(s) 211, 541, 860
BseRI GAGGAG 2 cut(s) 95, 98
BsgI GTGCAG 2 cut(s) 326, 709
BshFI GGCC 2 cut(s) 576, 835
BsiHKAI GWGCWC 1 cut(s) 793
BsiSI CCGG 1 cut(s) 162
BslI CCNNNNNNNGG 2 cut(s) 322, 323
BsmAI GTCTC 4 cut(s) 282, 657, 794, 850
BsmI GAATGC 1 cut(s) 463
BsnI GGCC 2 cut(s) 576, 835
Bso31I GGTCTC 1 cut(s) 850
Bsp1286I GDGCHC 1 cut(s) 793
Bsp143I GATC 3 cut(s) 286, 491, 583
BspACI CCGC 1 cut(s) 147
BspANI GGCC 2 cut(s) 576, 835
BspCNI CTCAG 2 cut(s) 721, 853
BspHI TCATGA 1 cut(s) 808
BspLI GGNNCC 1 cut(s) 100
BspMI ACCTGC 1 cut(s) 298
BspPI GGATC 2 cut(s) 499, 578
BspTNI GGTCTC 1 cut(s) 850
BsrI ACTGG 3 cut(s) 211, 541, 860
BssECI CCNNGG 2 cut(s) 162, 878
BssMI GATC 3 cut(s) 286, 491, 583
BssNAI GTATAC 1 cut(s) 565
Bst1107I GTATAC 1 cut(s) 565
Bst2UI CCWGG 2 cut(s) 660, 879
Bst4CI ACNGT 4 cut(s) 391, 715, 769, 805
Bst6I CTCTTC 1 cut(s) 627
BstDEI CTNAG 3 cut(s) 729, 794, 840
BstKTI GATC 3 cut(s) 289, 494, 586
BstMAI GTCTC 4 cut(s) 282, 657, 794, 850
BstMBI GATC 3 cut(s) 286, 491, 583
BstMWI GCNNNNNNNGC 1 cut(s) 841
BstNI CCWGG 2 cut(s) 660, 879
BstPAI GACNNNNGTC 1 cut(s) 749
BstSCI CCNGG 3 cut(s) 161, 658, 877
BstV2I GAAGAC 1 cut(s) 411
BstXI CCANNNNNNTGG 1 cut(s) 225
BstZ17I GTATAC 1 cut(s) 565
BsuRI GGCC 2 cut(s) 576, 835
BtsIMutI CAGTG 3 cut(s) 711, 726, 867
BveI ACCTGC 1 cut(s) 298
CciI TCATGA 1 cut(s) 808
Cfr13I GGNCC 1 cut(s) 215
CseI GACGC 1 cut(s) 416
Csp6I GTAC 1 cut(s) 432
CviAII CATG 2 cut(s) 380, 809
CviJI RGCY 6 cut(s) 146, 353, 531, 576, 601, 835
CviKI_1 RGCY 6 cut(s) 146, 353, 531, 576, 601, 835
CviQI GTAC 1 cut(s) 432
DdeI CTNAG 3 cut(s) 729, 794, 840
DpnI GATC 3 cut(s) 288, 493, 585
DpnII GATC 3 cut(s) 286, 491, 583
EaeI YGGCCR 1 cut(s) 574
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
Eco31I GGTCTC 1 cut(s) 850
Eco47I GGWCC 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 658, 877
FaeI CATG 2 cut(s) 383, 812
FalI AAGNNNNNCTT 2 cut(s) 487, 519
FatI CATG 2 cut(s) 379, 808
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 1 cut(s) 147
Fsp4HI GCNGC 1 cut(s) 147
GluI GCNGC 1 cut(s) 147
GsuI CTGGAG 1 cut(s) 861
HaeIII GGCC 2 cut(s) 576, 835
HapII CCGG 1 cut(s) 162
HgaI GACGC 1 cut(s) 416
Hin1II CATG 2 cut(s) 383, 812
HinfI GANTC 1 cut(s) 158
HpaII CCGG 1 cut(s) 162
HphI GGTGA 5 cut(s) 124, 185, 403, 459, 772
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 1 cut(s) 475
Hpy188III TCNNGA 3 cut(s) 293, 367, 809
Hpy8I GTNNAC 1 cut(s) 565
HpyAV CCTTC 2 cut(s) 480, 598
HpyCH4III ACNGT 4 cut(s) 391, 715, 769, 805
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 6 cut(s) 141, 274, 307, 514, 726, 830
HpyF10VI GCNNNNNNNGC 1 cut(s) 841
HpyF3I CTNAG 3 cut(s) 729, 794, 840
HpySE526I ACGT 1 cut(s) 516
Hsp92II CATG 2 cut(s) 383, 812
Kzo9I GATC 3 cut(s) 286, 491, 583
LmnI GCTCC 1 cut(s) 778
MaeII ACGT 1 cut(s) 516
MalI GATC 3 cut(s) 288, 493, 585
MboI GATC 3 cut(s) 286, 491, 583
MboII GAAGA 6 cut(s) 34, 70, 73, 281, 416, 644
MhlI GDGCHC 1 cut(s) 793
MluCI AATT 6 cut(s) 12, 234, 329, 558, 738, 845
MlyI GAGTC 1 cut(s) 152
MseI TTAA 2 cut(s) 426, 654
MslI CAYNNNNRTG 1 cut(s) 389
MspI CCGG 1 cut(s) 162
MspR9I CCNGG 3 cut(s) 163, 660, 879
Mva1269I GAATGC 1 cut(s) 463
MvaI CCWGG 2 cut(s) 660, 879
MwoI GCNNNNNNNGC 1 cut(s) 841
NciI CCSGG 1 cut(s) 163
NdeII GATC 3 cut(s) 286, 491, 583
NlaIII CATG 2 cut(s) 383, 812
NlaIV GGNNCC 1 cut(s) 100
PagI TCATGA 1 cut(s) 808
PctI GAATGC 1 cut(s) 463
PfoI TCCNGGA 1 cut(s) 658
PkrI GCNGC 1 cut(s) 148
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
PshAI GACNNNNGTC 1 cut(s) 749
PshBI ATTAAT 2 cut(s) 426, 654
Psp6I CCWGG 2 cut(s) 658, 877
PspGI CCWGG 2 cut(s) 658, 877
PspN4I GGNNCC 1 cut(s) 100
PspPI GGNCC 1 cut(s) 215
RsaI GTAC 1 cut(s) 433
RsaNI GTAC 1 cut(s) 432
RseI CAYNNNNRTG 1 cut(s) 389
SaqAI TTAA 2 cut(s) 426, 654
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 3 cut(s) 286, 491, 583
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 3 cut(s) 163, 660, 879
SduI GDGCHC 1 cut(s) 793
SinI GGWCC 1 cut(s) 215
SmiMI CAYNNNNRTG 1 cut(s) 389
Sse9I AATT 6 cut(s) 12, 234, 329, 558, 738, 845
SsiI CCGC 1 cut(s) 147
SspI AATATT 1 cut(s) 424
StyD4I CCNGG 3 cut(s) 161, 658, 877
TaaI ACNGT 4 cut(s) 391, 715, 769, 805
TaiI ACGT 1 cut(s) 519
TasI AATT 6 cut(s) 12, 234, 329, 558, 738, 845
TauI GCSGC 1 cut(s) 149
Tru1I TTAA 2 cut(s) 426, 654
Tru9I TTAA 2 cut(s) 426, 654
TscAI CASTG 3 cut(s) 718, 733, 867
TspDTI ATGAA 3 cut(s) 192, 512, 797
TspRI CASTG 3 cut(s) 718, 733, 867
VpaK11BI GGWCC 1 cut(s) 215
VspI ATTAAT 2 cut(s) 426, 654
XmiI GTMKAC 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.