MD12G1083000.v1.1

carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012107
Physical Location & Seq
Reverse (-)
10077185 .. 10080845
3661 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1083000.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGGGAAGGGAAGGGCAGACAATCCATCTCCAATTACTTGTTACAGAAATCCTCTACTGGTCTGAAAAATGCTTTGTCTTCCCAATACGCTGCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTACATATTTATATCTCTTCTTCTGACATGCCATTTCATTCCGACTCCTCCCAATCCAATCACCAAAACCAAATTTATGAGCTTGTTCTACGTCGCCATGACCAAACCCAACTTCTTCTTTCTCTTTCTTGCGTCTCTCCTCTTCTCGTCTCATCACTCCTCCTTCACCATTTGCAGTGCAGCAGTCGAGGACTCTGATGAAGTTGAAAGTGATGAAACTCCATTTACTTATCTTGAAGGAACTGGTAAAGGACCAAAGAAATGGGGAGAAATTGACCCTCATTGGAAAGTTTGTGATACTGGAAAATTGCAGTCTCCAATCGATCTTCTTGACCAAAGGGTGCAGGTTTTCCCTCACTTGGGGAAATTGAAAAGGGGTTACAAACCAGCTCCTGCTACTGTGAAGAACCGAGGTCATGACATCACGATGAGGTGGAACGGAGATGCAGGAAAAATCAACATAAATGGGACTGATTATAGACTGTTACAATGTCATTGGCATTCACCCTCTGAACATACGTTTAATGGATCAAGGTATGCTTTGGAGTTTCATGTTGTTCATTTAAGCTCTACCGGAAAGATTGCTGTAATTGGAATTGTGTACAAATATGGTCGACCTGATCCCCTCCTTTCAAAGCTATTCCACCACATAAAATCGGTTGGGATAGAAGAGGAGGTTGACATAGGGATTATTAATCCCGGGGATATTAAGTTCGGAAGCAGAAAGTACTACAGATATATCGGTTCTCTTACCGTTCCTCCGTGCACTGAGGGTGTAATTTGGACAATCGTCAAAAAGGTGAGGACAGTTTCAAGGGAGCAAGTGCATGCACTAAGGGAGGCTGTACATGATGGATTCGAGATGAATGCAAGGCCAACTCAAGAACTGGATGGAAGACCAGTGCTGTTATACACTCCAAGGGACATTGGAGGTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase LSM

Protein Analysis

361

Amino Acids

40.6

Weight (kDa)

8.56

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 131 - 349 1.4e-39 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012107)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20990
fragaria_vesca FvH4_6g24590 FvH4_6g24590 FvH4_6g24590
malus_domestica MD12G1083000.v1.1 MD14G1078100.v1.1
prunus_persica Prupe.7G052800_v2.0.a1
pyrus_communis pycom12g06990 pycom14g06650
rosa_chinensis RchiOBHm_Chr3g0483901
rosa_laevigata RLG00000023253
rosa_multiflora Rmu_sc0002252.1_g000013
rosa_roxburghii Rroxscaffold_6G00398060
rosa_rugosa Rorug03G0208600
rosa_samantha Rh3AG257300 Rh3BG293700 Rh3CG292000 Rh3DG287100
rosa_wichuraiana Rw3G023260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 475
AccI GTMKAC 1 cut(s) 754
AclWI GGATC 2 cut(s) 676, 755
AcsI RAATTY 1 cut(s) 212
AfaI GTAC 3 cut(s) 743, 869, 987
AfiI CCNNNNNNNGG 2 cut(s) 499, 500
AgsI TTSAA 5 cut(s) 347, 377, 511, 774, 954
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AloI GAACNNNNNNTCC 2 cut(s) 836, 868
AluBI AGCT 4 cut(s) 222, 530, 708, 778
AluI AGCT 4 cut(s) 222, 530, 708, 778
Alw21I GWGCWC 1 cut(s) 908
Alw26I GTCTC 3 cut(s) 279, 294, 459
Alw44I GTGCAC 1 cut(s) 904
AlwI GGATC 2 cut(s) 676, 755
AlwNI CAGNNNCTG 1 cut(s) 533
Ama87I CYCGRG 1 cut(s) 839
AoxI GGCC 1 cut(s) 1013
ApaLI GTGCAC 1 cut(s) 904
ApeKI GCWGC 2 cut(s) 90, 320
ApoI RAATTY 1 cut(s) 212
AseI ATTAAT 1 cut(s) 834
AspS9I GGNCC 1 cut(s) 392
AsuC2I CCSGG 2 cut(s) 840, 841
AsuHPI GGTGA 4 cut(s) 193, 298, 636, 952
AvaI CYCGRG 1 cut(s) 839
AvaII GGWCC 1 cut(s) 392
BaeGI GKGCMC 1 cut(s) 908
BbsI GAAGAC 2 cut(s) 70, 1042
Bbv12I GWGCWC 1 cut(s) 908
BbvI GCAGC 2 cut(s) 77, 332
BccI CCATC 3 cut(s) 33, 986, 1025
BcgI CGANNNNNNTGC 2 cut(s) 989, 1023
BcnI CCSGG 2 cut(s) 840, 841
BcoDI GTCTC 3 cut(s) 279, 294, 459
BfmI CTRYAG 1 cut(s) 871
BfuAI ACCTGC 1 cut(s) 475
BisI GCNGC 2 cut(s) 91, 321
BlsI GCNGC 2 cut(s) 92, 322
BmcAI AGTACT 1 cut(s) 869
Bme1390I CCNGG 2 cut(s) 840, 841
Bme18I GGWCC 1 cut(s) 392
BmeT110I CYCGRG 1 cut(s) 839
BmgT120I GGNCC 1 cut(s) 392
BmrFI CCNGG 2 cut(s) 840, 841
BmsI GCATC 1 cut(s) 574
BoxI GACNNNNGTC 1 cut(s) 929
BpiI GAAGAC 2 cut(s) 70, 1042
BpuEI CTTGAG 1 cut(s) 1005
BpuMI CCSGG 2 cut(s) 840, 841
Bsa29I ATCGAT 1 cut(s) 462
BsaJI CCNNGG 4 cut(s) 550, 839, 840, 1058
BsaWI WCCGGW 1 cut(s) 713
BsaXI ACNNNNNCTCC 2 cut(s) 883, 913
Bsc4I CCNNNNNNNGG 2 cut(s) 499, 500
Bse1I ACTGG 5 cut(s) 62, 388, 445, 1032, 1040
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 4 cut(s) 550, 839, 840, 1058
BseGI GGATG 1 cut(s) 1036
BseLI CCNNNNNNNGG 2 cut(s) 499, 500
BseMII CTCAG 1 cut(s) 900
BseNI ACTGG 5 cut(s) 62, 388, 445, 1032, 1040
BseRI GAGGAG 4 cut(s) 177, 269, 289, 827
BseSI GKGCMC 1 cut(s) 908
BseXI GCAGC 2 cut(s) 77, 332
BsgI GTGCAG 2 cut(s) 339, 503
BshFI GGCC 1 cut(s) 1015
BshVI ATCGAT 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 908
BsiHKCI CYCGRG 1 cut(s) 839
BsiSI CCGG 2 cut(s) 714, 840
BslFI GGGAC 2 cut(s) 622, 1076
BslI CCNNNNNNNGG 2 cut(s) 499, 500
BsmAI GTCTC 3 cut(s) 279, 294, 459
BsmBI CGTCTC 2 cut(s) 279, 294
BsmFI GGGAC 2 cut(s) 622, 1076
BsmI GAATGC 2 cut(s) 640, 1012
BsnI GGCC 1 cut(s) 1015
BsoBI CYCGRG 1 cut(s) 839
Bsp1286I GDGCHC 1 cut(s) 908
Bsp1407I TGTACA 2 cut(s) 741, 985
Bsp143I GATC 3 cut(s) 463, 668, 760
BspANI GGCC 1 cut(s) 1015
BspCNI CTCAG 1 cut(s) 901
BspDI ATCGAT 1 cut(s) 462
BspHI TCATGA 1 cut(s) 556
BspMI ACCTGC 1 cut(s) 475
BspPI GGATC 2 cut(s) 676, 755
BsrGI TGTACA 2 cut(s) 741, 985
BsrI ACTGG 5 cut(s) 62, 388, 445, 1032, 1040
BssECI CCNNGG 4 cut(s) 550, 839, 840, 1058
BssMI GATC 3 cut(s) 463, 668, 760
BssT1I CCWWGG 1 cut(s) 1058
Bst4CI ACNGT 4 cut(s) 541, 624, 895, 949
Bst6I CTCTTC 3 cut(s) 162, 287, 804
BstAUI TGTACA 2 cut(s) 741, 985
BstC8I GCNNGC 1 cut(s) 969
BstDEI CTNAG 2 cut(s) 909, 974
BstF5I GGATG 1 cut(s) 1036
BstKTI GATC 3 cut(s) 466, 671, 763
BstMAI GTCTC 3 cut(s) 279, 294, 459
BstMBI GATC 3 cut(s) 463, 668, 760
BstNSI RCATGY 2 cut(s) 171, 971
BstPAI GACNNNNGTC 1 cut(s) 929
BstSCI CCNGG 2 cut(s) 838, 839
BstSFI CTRYAG 1 cut(s) 871
BstSLI GKGCMC 1 cut(s) 908
BstV1I GCAGC 2 cut(s) 77, 332
BstV2I GAAGAC 2 cut(s) 70, 1042
BstXI CCANNNNNNTGG 1 cut(s) 402
Bsu15I ATCGAT 1 cut(s) 462
BsuRI GGCC 1 cut(s) 1015
BsuTUI ATCGAT 1 cut(s) 462
BtsCI GGATG 1 cut(s) 1036
BtsI GCAGTG 1 cut(s) 322
BtsIMutI CAGTG 3 cut(s) 322, 906, 1047
BveI ACCTGC 1 cut(s) 475
Cac8I GCNNGC 1 cut(s) 969
CaiI CAGNNNCTG 1 cut(s) 533
CciI TCATGA 1 cut(s) 556
Cfr13I GGNCC 1 cut(s) 392
Cfr9I CCCGGG 1 cut(s) 839
ClaI ATCGAT 1 cut(s) 462
CseI GACGC 1 cut(s) 261
Csp6I GTAC 3 cut(s) 742, 868, 986
CviAII CATG 6 cut(s) 168, 238, 557, 692, 968, 989
CviJI RGCY 6 cut(s) 222, 530, 708, 778, 983, 1015
CviKI_1 RGCY 6 cut(s) 222, 530, 708, 778, 983, 1015
CviQI GTAC 3 cut(s) 742, 868, 986
DdeI CTNAG 2 cut(s) 909, 974
DpnI GATC 3 cut(s) 465, 670, 762
DpnII GATC 3 cut(s) 463, 668, 760
Eam1104I CTCTTC 3 cut(s) 162, 287, 804
EarI CTCTTC 3 cut(s) 162, 287, 804
Eco130I CCWWGG 1 cut(s) 1058
Eco47I GGWCC 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 839
EcoT14I CCWWGG 1 cut(s) 1058
ErhI CCWWGG 1 cut(s) 1058
Esp3I CGTCTC 2 cut(s) 279, 294
FaeI CATG 6 cut(s) 171, 241, 560, 695, 971, 992
FalI AAGNNNNNCTT 2 cut(s) 664, 696
FaqI GGGAC 2 cut(s) 622, 1076
FatI CATG 6 cut(s) 167, 237, 556, 691, 967, 988
FblI GTMKAC 1 cut(s) 754
Fnu4HI GCNGC 2 cut(s) 91, 321
FokI GGATG 1 cut(s) 1043
Fsp4HI GCNGC 2 cut(s) 91, 321
GluI GCNGC 2 cut(s) 91, 321
HaeIII GGCC 1 cut(s) 1015
HapII CCGG 2 cut(s) 714, 840
HgaI GACGC 1 cut(s) 261
Hin1II CATG 6 cut(s) 171, 241, 560, 695, 971, 992
HincII GTYRAC 2 cut(s) 755, 820
HindII GTYRAC 2 cut(s) 755, 820
HinfI GANTC 3 cut(s) 184, 332, 996
HpaII CCGG 2 cut(s) 714, 840
HphI GGTGA 4 cut(s) 193, 298, 636, 952
Hpy166II GTNNAC 4 cut(s) 742, 755, 820, 906
Hpy188I TCNGA 6 cut(s) 64, 165, 183, 337, 652, 857
Hpy188III TCNNGA 6 cut(s) 374, 470, 557, 565, 1000, 1022
Hpy8I GTNNAC 4 cut(s) 742, 755, 820, 906
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 3 cut(s) 5, 313, 371
HpyCH4III ACNGT 4 cut(s) 541, 624, 895, 949
HpyCH4IV ACGT 2 cut(s) 231, 659
HpyCH4V TGCA 9 cut(s) 315, 320, 451, 484, 587, 906, 967, 971, 1010
HpyF3I CTNAG 2 cut(s) 909, 974
HpySE526I ACGT 2 cut(s) 231, 659
Hsp92II CATG 6 cut(s) 171, 241, 560, 695, 971, 992
Kzo9I GATC 3 cut(s) 463, 668, 760
LmnI GCTCC 2 cut(s) 535, 958
Lsp1109I GCAGC 2 cut(s) 77, 332
LweI GCATC 1 cut(s) 574
MaeII ACGT 2 cut(s) 231, 659
MaeIII GTNAC 3 cut(s) 40, 518, 624
MalI GATC 3 cut(s) 465, 670, 762
MboI GATC 3 cut(s) 463, 668, 760
MboII GAAGA 9 cut(s) 70, 149, 152, 247, 274, 458, 556, 821, 1047
MhlI GDGCHC 1 cut(s) 908
MluCI AATT 8 cut(s) 32, 212, 411, 446, 506, 729, 735, 918
MlyI GAGTC 2 cut(s) 178, 326
MmeI TCCRAC 1 cut(s) 206
MseI TTAA 5 cut(s) 663, 704, 834, 849, 1081
MslI CAYNNNNRTG 1 cut(s) 566
MspI CCGG 2 cut(s) 714, 840
MspR9I CCNGG 2 cut(s) 840, 841
Mva1269I GAATGC 2 cut(s) 640, 1012
NciI CCSGG 2 cut(s) 840, 841
NdeII GATC 3 cut(s) 463, 668, 760
NlaIII CATG 6 cut(s) 171, 241, 560, 695, 971, 992
NspI RCATGY 2 cut(s) 171, 971
PaeI GCATGC 1 cut(s) 971
PagI TCATGA 1 cut(s) 556
PctI GAATGC 2 cut(s) 640, 1012
PfeI GAWTC 1 cut(s) 996
PkrI GCNGC 2 cut(s) 92, 322
PleI GAGTC 2 cut(s) 178, 326
PpsI GAGTC 2 cut(s) 178, 326
PshAI GACNNNNGTC 1 cut(s) 929
PshBI ATTAAT 1 cut(s) 834
PspPI GGNCC 1 cut(s) 392
PstNI CAGNNNCTG 1 cut(s) 533
RsaI GTAC 3 cut(s) 743, 869, 987
RsaNI GTAC 3 cut(s) 742, 868, 986
RseI CAYNNNNRTG 1 cut(s) 566
SalI GTCGAC 1 cut(s) 753
SaqAI TTAA 5 cut(s) 663, 704, 834, 849, 1081
SatI GCNGC 2 cut(s) 91, 321
Sau3AI GATC 3 cut(s) 463, 668, 760
Sau96I GGNCC 1 cut(s) 392
ScaI AGTACT 1 cut(s) 869
SchI GAGTC 2 cut(s) 178, 326
ScrFI CCNGG 2 cut(s) 840, 841
SduI GDGCHC 1 cut(s) 908
SfaNI GCATC 1 cut(s) 574
SfcI CTRYAG 1 cut(s) 871
SinI GGWCC 1 cut(s) 392
SmaI CCCGGG 1 cut(s) 841
SmiMI CAYNNNNRTG 1 cut(s) 566
SmlI CTYRAG 1 cut(s) 1020
SmoI CTYRAG 1 cut(s) 1020
SphI GCATGC 1 cut(s) 971
Sse9I AATT 8 cut(s) 32, 212, 411, 446, 506, 729, 735, 918
StyD4I CCNGG 2 cut(s) 838, 839
StyI CCWWGG 1 cut(s) 1058
TaaI ACNGT 4 cut(s) 541, 624, 895, 949
TaiI ACGT 2 cut(s) 234, 662
TaqI TCGA 4 cut(s) 327, 462, 754, 999
TasI AATT 8 cut(s) 32, 212, 411, 446, 506, 729, 735, 918
TatI WGTACW 3 cut(s) 741, 867, 985
TfiI GAWTC 1 cut(s) 996
Tru1I TTAA 5 cut(s) 663, 704, 834, 849, 1081
Tru9I TTAA 5 cut(s) 663, 704, 834, 849, 1081
TscAI CASTG 3 cut(s) 322, 913, 1047
TseI GCWGC 2 cut(s) 90, 320
TspDTI ATGAA 6 cut(s) 166, 354, 369, 680, 689, 1019
TspGWI ACGGA 2 cut(s) 594, 891
TspMI CCCGGG 1 cut(s) 839
TspRI CASTG 3 cut(s) 322, 913, 1047
VneI GTGCAC 1 cut(s) 904
VpaK11BI GGWCC 1 cut(s) 392
VspI ATTAAT 1 cut(s) 834
XapI RAATTY 1 cut(s) 212
XceI RCATGY 2 cut(s) 171, 971
XmaI CCCGGG 1 cut(s) 839
XmiI GTMKAC 1 cut(s) 754
ZrmI AGTACT 1 cut(s) 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.