RLG00000023253

carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012107
Physical Location & Seq
Reverse (-)
20666778 .. 20670732
3955 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023253

Sequence Viewer

Length: 894 bp
ATGCCATTTCCAATTTTGAAGCAACGAAGCATTATGTGGTTCTTCAACCCCAACCCCAAACCCAACTTGTTCTTCTTTCTCATTTTGGGTTCCCTCCTCCTCTTATCCCATCATTCCTTTTTCACCATCTGCAGAGCCGCACTTGATGACTCCGGGGAAGTTGGTGATGAACTTCCATTTACTTATGTTGAGGGAACTGGTAAAGGACCAAAGAAATGGGGTCAAATTGACCCGGATTGGAAAGTTTGTGATAATGGAAAAATGCAGTCTCCTATTGATCTTCTTGACGGTAGGGTGCAGGTTTTCCCTAACTTGGGGAAGTTGAAAAGGGATTACAACCAAGCCCCTGCAACTATCAAGAACAGAGGGCATGACATTACGGTGAGGTGGAAAGAAGACGCAGGGAAAATCAATATTAATGGTACAGACTATAATCTGCTACAATGTCATTGGCATTCACCTTCTGAACACACATTCAATGGATCAAGGTATGCTTTGGAGCTTCACGTTGTTCATACAAGCTCTACTGGAGAGATTGCTGTTATTGGAATTGTATACAAATATGGCCGACCTGATATCTTCCTTTCAAAGCTGTTCCACCACATAAAATCGGTTGGGAAAGAAGAGGTTGATATTGGCATTATTAATCCAGGAGACATCAAGTTTGGAAGCAGAAAGTATTACAGATATATTGGTTCTCTCACTGTTCCTCCCTGCACTGAGGGTGTAATTTGGACAATAGTCAAAAAGGTGAGGACAGTTTCAAGGGAGCAAGTGAGAGCACTAAGGGAGACTGTTCATGATGGATATGAGGAAAATGCAAGGCCAACTCAGCAATTCAGTGGGAGACCAGTGTTGCTATACACTCCAGGGGAGAATGGAGGTTCTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase LSM

Protein Analysis

298

Amino Acids

33.56

Weight (kDa)

8.89

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 69 - 286 1.2e-41 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012107)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20990
fragaria_vesca FvH4_6g24590 FvH4_6g24590 FvH4_6g24590
malus_domestica MD12G1083000.v1.1 MD14G1078100.v1.1
prunus_persica Prupe.7G052800_v2.0.a1
pyrus_communis pycom12g06990 pycom14g06650
rosa_chinensis RchiOBHm_Chr3g0483901
rosa_laevigata RLG00000023253
rosa_multiflora Rmu_sc0002252.1_g000013
rosa_roxburghii Rroxscaffold_6G00398060
rosa_rugosa Rorug03G0208600
rosa_samantha Rh3AG257300 Rh3BG293700 Rh3CG292000 Rh3DG287100
rosa_wichuraiana Rw3G023260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 289
AccI GTMKAC 1 cut(s) 555
AciI CCGC 1 cut(s) 138
AclWI GGATC 1 cut(s) 490
AcoI YGGCCR 1 cut(s) 565
AfaI GTAC 1 cut(s) 424
AfiI CCNNNNNNNGG 2 cut(s) 313, 314
AgsI TTSAA 6 cut(s) 19, 46, 325, 478, 588, 765
AjnI CCWGG 2 cut(s) 649, 868
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 3 cut(s) 502, 522, 592
AluI AGCT 3 cut(s) 502, 522, 592
Alw21I GWGCWC 1 cut(s) 784
Alw26I GTCTC 4 cut(s) 273, 648, 785, 841
AlwI GGATC 1 cut(s) 490
AoxI GGCC 2 cut(s) 565, 824
ArsI GACNNNNNNTTYG 2 cut(s) 647, 679
AseI ATTAAT 2 cut(s) 417, 645
AspS9I GGNCC 1 cut(s) 206
AsuC2I CCSGG 2 cut(s) 154, 233
AsuHPI GGTGA 5 cut(s) 115, 176, 394, 450, 763
AvaII GGWCC 1 cut(s) 206
BbsI GAAGAC 1 cut(s) 402
Bbv12I GWGCWC 1 cut(s) 784
BccI CCATC 3 cut(s) 117, 134, 797
BciT130I CCWGG 2 cut(s) 651, 870
BcnI CCSGG 2 cut(s) 154, 233
BcoDI GTCTC 4 cut(s) 273, 648, 785, 841
BfmI CTRYAG 1 cut(s) 130
BfuAI ACCTGC 1 cut(s) 289
BisI GCNGC 1 cut(s) 138
BlsI GCNGC 1 cut(s) 139
Bme1390I CCNGG 4 cut(s) 154, 233, 651, 870
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 4 cut(s) 154, 233, 651, 870
BoxI GACNNNNGTC 1 cut(s) 740
BpiI GAAGAC 1 cut(s) 402
BpmI CTGGAG 2 cut(s) 549, 852
BpuMI CCSGG 2 cut(s) 154, 233
BsaI GGTCTC 1 cut(s) 841
BsaJI CCNNGG 2 cut(s) 153, 869
BsaXI ACNNNNNCTCC 2 cut(s) 694, 724
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 314
Bse1I ACTGG 3 cut(s) 202, 532, 851
BseBI CCWGG 2 cut(s) 651, 870
BseDI CCNNGG 2 cut(s) 153, 869
BseLI CCNNNNNNNGG 2 cut(s) 313, 314
BseMII CTCAG 2 cut(s) 711, 845
BseNI ACTGG 3 cut(s) 202, 532, 851
BseRI GAGGAG 2 cut(s) 86, 89
BsgI GTGCAG 2 cut(s) 317, 700
BshFI GGCC 2 cut(s) 567, 826
BsiHKAI GWGCWC 1 cut(s) 784
BsiSI CCGG 2 cut(s) 153, 233
BslI CCNNNNNNNGG 2 cut(s) 313, 314
BsmAI GTCTC 4 cut(s) 273, 648, 785, 841
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 2 cut(s) 567, 826
Bso31I GGTCTC 1 cut(s) 841
Bsp1286I GDGCHC 1 cut(s) 784
Bsp143I GATC 2 cut(s) 277, 482
BspACI CCGC 1 cut(s) 138
BspANI GGCC 2 cut(s) 567, 826
BspCNI CTCAG 2 cut(s) 712, 844
BspHI TCATGA 1 cut(s) 799
BspLI GGNNCC 1 cut(s) 91
BspMAI CTGCAG 1 cut(s) 134
BspMI ACCTGC 1 cut(s) 289
BspPI GGATC 1 cut(s) 490
BspTNI GGTCTC 1 cut(s) 841
BsrI ACTGG 3 cut(s) 202, 532, 851
BssECI CCNNGG 2 cut(s) 153, 869
BssMI GATC 2 cut(s) 277, 482
BssNAI GTATAC 1 cut(s) 556
Bst1107I GTATAC 1 cut(s) 556
Bst2UI CCWGG 2 cut(s) 651, 870
Bst4CI ACNGT 5 cut(s) 290, 382, 706, 760, 796
Bst6I CTCTTC 1 cut(s) 618
BstDEI CTNAG 3 cut(s) 720, 785, 831
BstKTI GATC 2 cut(s) 280, 485
BstMAI GTCTC 4 cut(s) 273, 648, 785, 841
BstMBI GATC 2 cut(s) 277, 482
BstMWI GCNNNNNNNGC 1 cut(s) 832
BstNI CCWGG 2 cut(s) 651, 870
BstPAI GACNNNNGTC 1 cut(s) 740
BstSCI CCNGG 4 cut(s) 152, 231, 649, 868
BstSFI CTRYAG 1 cut(s) 130
BstV2I GAAGAC 1 cut(s) 402
BstXI CCANNNNNNTGG 1 cut(s) 216
BstZ17I GTATAC 1 cut(s) 556
BsuRI GGCC 2 cut(s) 567, 826
BtsIMutI CAGTG 4 cut(s) 702, 717, 847, 858
BveI ACCTGC 1 cut(s) 289
CciI TCATGA 1 cut(s) 799
Cfr13I GGNCC 1 cut(s) 206
CseI GACGC 1 cut(s) 407
Csp6I GTAC 1 cut(s) 423
CviAII CATG 2 cut(s) 371, 800
CviJI RGCY 7 cut(s) 137, 344, 502, 522, 567, 592, 826
CviKI_1 RGCY 7 cut(s) 137, 344, 502, 522, 567, 592, 826
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 3 cut(s) 720, 785, 831
DpnI GATC 2 cut(s) 279, 484
DpnII GATC 2 cut(s) 277, 482
EaeI YGGCCR 1 cut(s) 565
Eam1104I CTCTTC 1 cut(s) 618
EarI CTCTTC 1 cut(s) 618
Eco31I GGTCTC 1 cut(s) 841
Eco32I GATATC 1 cut(s) 577
Eco47I GGWCC 1 cut(s) 206
EcoRII CCWGG 2 cut(s) 649, 868
EcoRV GATATC 1 cut(s) 577
FaeI CATG 2 cut(s) 374, 803
FalI AAGNNNNNCTT 2 cut(s) 478, 510
FatI CATG 2 cut(s) 370, 799
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 1 cut(s) 138
Fsp4HI GCNGC 1 cut(s) 138
GluI GCNGC 1 cut(s) 138
GsuI CTGGAG 2 cut(s) 549, 852
HaeIII GGCC 2 cut(s) 567, 826
HapII CCGG 2 cut(s) 153, 233
HgaI GACGC 1 cut(s) 407
Hin1II CATG 2 cut(s) 374, 803
HinfI GANTC 1 cut(s) 149
HpaII CCGG 2 cut(s) 153, 233
HphI GGTGA 5 cut(s) 115, 176, 394, 450, 763
Hpy166II GTNNAC 1 cut(s) 556
Hpy188I TCNGA 1 cut(s) 466
Hpy188III TCNNGA 3 cut(s) 284, 358, 800
Hpy8I GTNNAC 1 cut(s) 556
HpyAV CCTTC 1 cut(s) 471
HpyCH4III ACNGT 5 cut(s) 290, 382, 706, 760, 796
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 6 cut(s) 132, 265, 298, 350, 717, 821
HpyF10VI GCNNNNNNNGC 1 cut(s) 832
HpyF3I CTNAG 3 cut(s) 720, 785, 831
HpySE526I ACGT 1 cut(s) 507
Hsp92II CATG 2 cut(s) 374, 803
Kzo9I GATC 2 cut(s) 277, 482
LmnI GCTCC 2 cut(s) 499, 769
MaeII ACGT 1 cut(s) 507
MalI GATC 2 cut(s) 279, 484
MboI GATC 2 cut(s) 277, 482
MboII GAAGA 6 cut(s) 34, 64, 272, 407, 571, 635
MhlI GDGCHC 1 cut(s) 784
MluCI AATT 5 cut(s) 12, 225, 549, 729, 836
MlyI GAGTC 1 cut(s) 143
MseI TTAA 2 cut(s) 417, 645
MslI CAYNNNNRTG 1 cut(s) 380
MspI CCGG 2 cut(s) 153, 233
MspR9I CCNGG 4 cut(s) 154, 233, 651, 870
Mva1269I GAATGC 1 cut(s) 454
MvaI CCWGG 2 cut(s) 651, 870
MwoI GCNNNNNNNGC 1 cut(s) 832
NciI CCSGG 2 cut(s) 154, 233
NdeII GATC 2 cut(s) 277, 482
NlaIII CATG 2 cut(s) 374, 803
NlaIV GGNNCC 1 cut(s) 91
PagI TCATGA 1 cut(s) 799
PctI GAATGC 1 cut(s) 454
PfoI TCCNGGA 1 cut(s) 649
PkrI GCNGC 1 cut(s) 139
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
PshAI GACNNNNGTC 1 cut(s) 740
PshBI ATTAAT 2 cut(s) 417, 645
Psp6I CCWGG 2 cut(s) 649, 868
PspGI CCWGG 2 cut(s) 649, 868
PspN4I GGNNCC 1 cut(s) 91
PspPI GGNCC 1 cut(s) 206
PstI CTGCAG 1 cut(s) 134
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
RseI CAYNNNNRTG 1 cut(s) 380
SaqAI TTAA 2 cut(s) 417, 645
SatI GCNGC 1 cut(s) 138
Sau3AI GATC 2 cut(s) 277, 482
Sau96I GGNCC 1 cut(s) 206
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 4 cut(s) 154, 233, 651, 870
SduI GDGCHC 1 cut(s) 784
SfcI CTRYAG 1 cut(s) 130
SinI GGWCC 1 cut(s) 206
SmiMI CAYNNNNRTG 1 cut(s) 380
Sse9I AATT 5 cut(s) 12, 225, 549, 729, 836
SsiI CCGC 1 cut(s) 138
SspI AATATT 1 cut(s) 415
StyD4I CCNGG 4 cut(s) 152, 231, 649, 868
TaaI ACNGT 5 cut(s) 290, 382, 706, 760, 796
TaiI ACGT 1 cut(s) 510
TasI AATT 5 cut(s) 12, 225, 549, 729, 836
TauI GCSGC 1 cut(s) 140
Tru1I TTAA 2 cut(s) 417, 645
Tru9I TTAA 2 cut(s) 417, 645
TscAI CASTG 4 cut(s) 709, 724, 847, 858
TspDTI ATGAA 3 cut(s) 183, 503, 788
TspRI CASTG 4 cut(s) 709, 724, 847, 858
VpaK11BI GGWCC 1 cut(s) 206
VspI ATTAAT 2 cut(s) 417, 645
XmiI GTMKAC 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.