AT4G21350

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012930
Physical Location & Seq
Reverse (-)
11356143 .. 11357267
1125 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G21350.1

Sequence Viewer

Length: 1125 bp
ATGGCCTTTGATTTACCAAACGATTTCAGATGCCCGATCTCCCTTGAGATCATGTCCGACCCAGTTATCCTCCAATCGGGTCATACTTTCGATCGGGTCTCTATCCAACAATGGATTGACTCCGGCAATCGAACTTGTCCAATCACCAAGCTACCCTTATCAGAAACCCCTTATCTGATTCCCAATCACGCACTTCGTAGCTTAATTTTAAACTTCGCCCATGTGAGTCTTAAGGAATCATCACGTCCTCGAACTCAACAAGAACACTCTCACTCTCAATCCCAAGCTTTAATCTCCACTCTCGTCTCTCAATCGTCGTCTAATGCATCAAAGCTCGAGTCTTTGACTCGACTCGTCCGACTCACGAAGCGAGACTCGTCAATCAGGAGGAAAGTTACTGAGTCAGGAGCAGTTCGTGCGGCTCTGGATTGTGTTGACTCTTGTAATCAGGTTTTACAAGAAAAGTCTCTGTCTTTGCTTTTGAATCTTAGTTTAGAAGATGATAACAAAGTTGGTTTAGTCGCTGATGGAGTGATAAGACGGATTGTTACTGTTTTACGAGTCGGGTCACCGGATTGTAAAGCGATTGCTGCTACTCTGTTAACGAGTTTAGCTGTTGTTGAAGTGAATAAAGCTACTATTGGATCATATCCTGATGCAATCTCTGCTCTTGTTTCTCTTCTCCGGGTAGGGAATGATAGGGAGAGAAAGGAATCAGCTACGGCTCTTTACGCTCTTTGCTCGTTTCCCGATAATCGGAAACGGGTTGTGGATTGTGGGTCGGTGCCTATTTTGGTTGAAGCTGCTGATTCAGGGTTAGAGAGAGCAGTGGAAGTGTTGGGACTTTTGGTTAAGTGTAGAGGAGGAAGAGAAGAGATGAGTAAAGTAAGTGGTTTTGTTGAGGTTTTGGTTAACGTTTTGAGGAATGGGAATTTGAAAGGGATTCAGTATTCTCTGTTTATCCTCAATTGCTTGTGTTGTTGTAGCGGAGAGATAGTTGATGAGGTTAAGAGAGAAGGTGTTGTAGAGATATGCTTTGGTTTTGAGGATAATGAGAGTGAGAAAATCAGAAGGAATGCTACTATCTTAGTTCATACTTTACTTGGGATTCCAATGAGTTCATGA

Protein Analysis

374

Amino Acids

40.87

Weight (kDa)

6.98

Isoelectric Point (pI)

47.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 6 - 73 7.8e-18 U-box domain
zf-Nse PF11789 8 - 48 9.8e-07 Zinc-finger of the MIZ type in Nse subunit
zf-RING_UBOX PF13445 11 - 47 5e-07 RING-type zinc-finger
ARM_ARMC5 PF24768 87 - 269 5.8e-10 ARMC5
ARM_PUB PF25598 103 - 363 1.2e-19 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012930)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21350
fragaria_vesca FvH4_3g03220
malus_domestica MD05G1334100.v1.1 MD10G1309000.v1.1
prunus_persica Prupe.4G031100_v2.0.a1 Prupe.4G031100_v2.0.a1
pyrus_communis pycom05g30580 pycom10g26040
rosa_chinensis RchiOBHm_Chr5g0004861
rosa_laevigata RLG00000031267
rosa_multiflora Rmu_sc0010871.1_g000001
rosa_roxburghii Rroxscaffold_1G00070760
rosa_rugosa Rorug04G0414800
rosa_samantha Rh5AG042300 Rh5BG042000 Rh5CG045800 Rh5DG042400
rosa_wichuraiana Rw5G004090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 784
AciI CCGC 2 cut(s) 419, 987
AclI AACGTT 1 cut(s) 915
AclWI GGATC 1 cut(s) 652
AcsI RAATTY 1 cut(s) 931
AfiI CCNNNNNNNGG 2 cut(s) 76, 756
AflII CTTAAG 1 cut(s) 230
AgsI TTSAA 4 cut(s) 484, 623, 800, 937
AjiI CACGTC 1 cut(s) 245
AluBI AGCT 8 cut(s) 151, 201, 287, 334, 614, 635, 719, 803
AluI AGCT 8 cut(s) 151, 201, 287, 334, 614, 635, 719, 803
Alw26I GTCTC 4 cut(s) 103, 310, 366, 471
AlwI GGATC 1 cut(s) 652
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 590, 803
ApoI RAATTY 1 cut(s) 931
ArsI GACNNNNNNTTYG 2 cut(s) 323, 355
AsuC2I CCSGG 1 cut(s) 686
AsuHPI GGTGA 2 cut(s) 136, 561
AvaI CYCGRG 1 cut(s) 335
BanI GGYRCC 1 cut(s) 784
BarI GAAGNNNNNNTAC 2 cut(s) 1063, 1095
BbvI GCAGC 2 cut(s) 577, 790
BccI CCATC 1 cut(s) 521
BceAI ACGGC 1 cut(s) 738
BcnI CCSGG 1 cut(s) 686
BcoDI GTCTC 4 cut(s) 103, 310, 366, 471
BfrI CTTAAG 1 cut(s) 230
BisI GCNGC 3 cut(s) 420, 591, 804
BlsI GCNGC 3 cut(s) 421, 592, 805
Bme1390I CCNGG 1 cut(s) 686
BmeT110I CYCGRG 1 cut(s) 335
BmgBI CACGTC 1 cut(s) 245
BmiI GGNNCC 1 cut(s) 786
BmrFI CCNGG 1 cut(s) 686
BmrI ACTGGG 1 cut(s) 56
BmsI GCATC 3 cut(s) 20, 335, 646
BmuI ACTGGG 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 65
BpuMI CCSGG 1 cut(s) 686
BsaI GGTCTC 1 cut(s) 103
BsaWI WCCGGW 1 cut(s) 571
BsaXI ACNNNNNCTCC 2 cut(s) 981, 1011
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 756
Bse1I ACTGG 1 cut(s) 62
BseLI CCNNNNNNNGG 2 cut(s) 76, 756
BseMII CTCAG 1 cut(s) 390
BseNI ACTGG 1 cut(s) 62
BseRI GAGGAG 1 cut(s) 876
BseXI GCAGC 2 cut(s) 577, 790
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 784
BsiEI CGRYCG 1 cut(s) 94
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 3 cut(s) 123, 572, 685
BslFI GGGAC 1 cut(s) 855
BslI CCNNNNNNNGG 2 cut(s) 76, 756
BsmAI GTCTC 4 cut(s) 103, 310, 366, 471
BsmBI CGTCTC 1 cut(s) 310
BsmFI GGGAC 1 cut(s) 855
BsmI GAATGC 1 cut(s) 1081
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 103
BsoBI CYCGRG 1 cut(s) 335
Bsp143I GATC 4 cut(s) 36, 48, 91, 644
BspACI CCGC 2 cut(s) 419, 987
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 391
BspHI TCATGA 1 cut(s) 1121
BspLI GGNNCC 1 cut(s) 786
BspPI GGATC 1 cut(s) 652
BspT107I GGYRCC 1 cut(s) 784
BspTI CTTAAG 1 cut(s) 230
BspTNI GGTCTC 1 cut(s) 103
BsrI ACTGG 1 cut(s) 62
BssMI GATC 4 cut(s) 36, 48, 91, 644
Bst4CI ACNGT 1 cut(s) 553
Bst6I CTCTTC 3 cut(s) 684, 862, 867
BstAFI CTTAAG 1 cut(s) 230
BstAPI GCANNNNNTGC 2 cut(s) 416, 665
BstDEI CTNAG 3 cut(s) 399, 488, 1087
BstEII GGTNACC 1 cut(s) 567
BstKTI GATC 4 cut(s) 39, 51, 94, 647
BstMAI GTCTC 4 cut(s) 103, 310, 366, 471
BstMBI GATC 4 cut(s) 36, 48, 91, 644
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 4 cut(s) 416, 590, 665, 731
BstPI GGTNACC 1 cut(s) 567
BstSCI CCNGG 1 cut(s) 684
BstV1I GCAGC 2 cut(s) 577, 790
BsuRI GGCC 1 cut(s) 5
BtrI CACGTC 1 cut(s) 245
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 1 cut(s) 834
CciI TCATGA 1 cut(s) 1121
CviAII CATG 3 cut(s) 52, 221, 1122
DdeI CTNAG 3 cut(s) 399, 488, 1087
DpnI GATC 4 cut(s) 38, 50, 93, 646
DpnII GATC 4 cut(s) 36, 48, 91, 644
DraI TTTAAA 1 cut(s) 210
Eam1104I CTCTTC 3 cut(s) 684, 862, 867
EarI CTCTTC 3 cut(s) 684, 862, 867
Eco31I GGTCTC 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 335
Eco91I GGTNACC 1 cut(s) 567
EcoO65I GGTNACC 1 cut(s) 567
EcoT22I ATGCAT 1 cut(s) 328
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 3 cut(s) 55, 224, 1125
FaiI YATR 7 cut(s) 53, 84, 222, 649, 1033, 1095, 1123
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FaqI GGGAC 1 cut(s) 855
FatI CATG 3 cut(s) 51, 220, 1121
Fnu4HI GCNGC 3 cut(s) 420, 591, 804
Fsp4HI GCNGC 3 cut(s) 420, 591, 804
GluI GCNGC 3 cut(s) 420, 591, 804
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 123, 572, 685
Hin1II CATG 3 cut(s) 55, 224, 1125
HincII GTYRAC 3 cut(s) 436, 603, 913
HindII GTYRAC 3 cut(s) 436, 603, 913
HindIII AAGCTT 1 cut(s) 285
HpaI GTTAAC 2 cut(s) 603, 913
HpaII CCGG 3 cut(s) 123, 572, 685
HphI GGTGA 2 cut(s) 136, 561
Hpy166II GTNNAC 3 cut(s) 436, 603, 913
Hpy188I TCNGA 7 cut(s) 29, 58, 163, 177, 359, 759, 1070
Hpy188III TCNNGA 7 cut(s) 364, 385, 405, 425, 653, 749, 1122
Hpy8I GTNNAC 3 cut(s) 436, 603, 913
Hpy99I CGWCG 1 cut(s) 319
HpyAV CCTTC 2 cut(s) 1010, 1065
HpyCH4III ACNGT 1 cut(s) 553
HpyCH4IV ACGT 2 cut(s) 244, 915
HpyCH4V TGCA 2 cut(s) 326, 659
HpyF10VI GCNNNNNNNGC 4 cut(s) 416, 590, 665, 731
HpyF3I CTNAG 3 cut(s) 399, 488, 1087
HpySE526I ACGT 2 cut(s) 244, 915
Hsp92II CATG 3 cut(s) 55, 224, 1125
KspAI GTTAAC 2 cut(s) 603, 913
Kzo9I GATC 4 cut(s) 36, 48, 91, 644
LmnI GCTCC 1 cut(s) 407
Lsp1109I GCAGC 2 cut(s) 577, 790
LweI GCATC 3 cut(s) 20, 335, 646
MaeII ACGT 2 cut(s) 244, 915
MaeIII GTNAC 3 cut(s) 394, 547, 567
MalI GATC 4 cut(s) 38, 50, 93, 646
MboI GATC 4 cut(s) 36, 48, 91, 644
MboII GAAGA 4 cut(s) 509, 671, 879, 884
MfeI CAATTG 1 cut(s) 967
MluCI AATT 3 cut(s) 204, 931, 967
MmeI TCCRAC 3 cut(s) 81, 130, 382
Mph1103I ATGCAT 1 cut(s) 328
MseI TTAA 8 cut(s) 203, 209, 231, 290, 602, 852, 912, 1008
MspCI CTTAAG 1 cut(s) 230
MspI CCGG 3 cut(s) 123, 572, 685
MspR9I CCNGG 1 cut(s) 686
MunI CAATTG 1 cut(s) 967
Mva1269I GAATGC 1 cut(s) 1081
MwoI GCNNNNNNNGC 4 cut(s) 416, 590, 665, 731
NciI CCSGG 1 cut(s) 686
NdeII GATC 4 cut(s) 36, 48, 91, 644
NlaIII CATG 3 cut(s) 55, 224, 1125
NlaIV GGNNCC 1 cut(s) 786
NmuCI GTSAC 1 cut(s) 567
NsiI ATGCAT 1 cut(s) 328
PaeR7I CTCGAG 1 cut(s) 335
PagI TCATGA 1 cut(s) 1121
PcsI WCGNNNNNNNCGW 1 cut(s) 355
PctI GAATGC 1 cut(s) 1081
PfeI GAWTC 7 cut(s) 178, 236, 484, 713, 809, 943, 1108
PkrI GCNGC 3 cut(s) 421, 592, 805
Ple19I CGATCG 1 cut(s) 94
Psp1406I AACGTT 1 cut(s) 915
PspEI GGTNACC 1 cut(s) 567
PspN4I GGNNCC 1 cut(s) 786
PspXI VCTCGAGB 1 cut(s) 335
PvuI CGATCG 1 cut(s) 94
SaqAI TTAA 8 cut(s) 203, 209, 231, 290, 602, 852, 912, 1008
SatI GCNGC 3 cut(s) 420, 591, 804
Sau3AI GATC 4 cut(s) 36, 48, 91, 644
ScrFI CCNGG 1 cut(s) 686
SfaNI GCATC 3 cut(s) 20, 335, 646
Sfr274I CTCGAG 1 cut(s) 335
SlaI CTCGAG 1 cut(s) 335
SmlI CTYRAG 3 cut(s) 44, 230, 335
SmoI CTYRAG 3 cut(s) 44, 230, 335
Sse9I AATT 3 cut(s) 204, 931, 967
SsiI CCGC 2 cut(s) 419, 987
StyD4I CCNGG 1 cut(s) 684
TaaI ACNGT 1 cut(s) 553
TaiI ACGT 2 cut(s) 247, 918
TaqI TCGA 5 cut(s) 90, 130, 250, 336, 349
TasI AATT 3 cut(s) 204, 931, 967
TauI GCSGC 1 cut(s) 422
TfiI GAWTC 7 cut(s) 178, 236, 484, 713, 809, 943, 1108
Tru1I TTAA 8 cut(s) 203, 209, 231, 290, 602, 852, 912, 1008
Tru9I TTAA 8 cut(s) 203, 209, 231, 290, 602, 852, 912, 1008
TscAI CASTG 1 cut(s) 834
TseFI GTSAC 1 cut(s) 567
TseI GCWGC 2 cut(s) 590, 803
Tsp45I GTSAC 1 cut(s) 567
TspDTI ATGAA 2 cut(s) 1082, 1110
TspGWI ACGGA 1 cut(s) 556
TspRI CASTG 1 cut(s) 834
Vha464I CTTAAG 1 cut(s) 230
XapI RAATTY 1 cut(s) 931
XhoI CTCGAG 1 cut(s) 335
Zsp2I ATGCAT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.