pycom10g26040

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012930
Physical Location & Seq
Forward (+)
27468448 .. 27469596
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g26040.1

Sequence Viewer

Length: 1149 bp
ATGGCGACTCAGTTCCCGGTCGATTTCAAGTGCCCGATTTCGCTCGAAATTATGTCCGACCCGGTTATACTCTCTTCGGGTCACACCTTTGATCGGGCCTCCATCCAACGCTGGCTCGACGCCGGCCACCGCACCTGCCCAATTTCCAAACTGCCGCTCCCCGACCACCCTTCCCTAATCCCCAACCACGCCCTCCGCAGCCTGATTTCCACTTATACCCTTCTCTCCCCTGCCAAATTACAACACCAGTACCACCCCCACCACCACCAATACCATCACCACCGCCAGCAGCGCCACTCCCAGCCCCAAACCCCAGTCTCGACGCTTACCTCCCCAGCTTCCACCCTCGAGTCCAAGCTCGACTCGCTCGACCAGCTCACTGGCCTCTCCAAGCGCGACGCCGCCTTCCGCGCCAAGCTCACCGAGTCCGGCGCCGTTTCCGCCGTCCTCAAGTGCGTTGACTCGGAGGAGCCCCGCCTCCAGGAAAAGGCTCTGACTTTGCTGCTCAATGTTAGCTTGGACGACGATAACAAAGTGGGTCTTGTCGCCGAGGGAGCAATCGCTCGAATCGTGGAGGTGCTCCAAGGTGGGTCCCCCAACAGCCGCGCCGTGGCGGCGACGATGTTGACTAGCTTGGCGGTGGTGGAGGTCAACAAGGCCACAATCGGGGCATACCCGTATGCAATTCGGGCTCTGGTTTCGCTTCTCAGGGACGGCAAGAGTCGAGAGCAGAAAGAGGCGGCAACGGCGCTTTACACCATCTGTTTGTTCCCAGATAATCGGCGGCGGGCGGTGGAATGTGGGGCGGTGTCTTTTTTGATCCGAATTGCTGAATCGGGCCTCGAACGGGCGGTTGAAGTTCTCGGTCTTTTGGCGAAGTGCAAGGAAGGTAGAGAAGAGATGGAGAGGTTTAATGGATGTGTGGGGATTTTAGTGAGGGTTTTAGAGAATGGGAGCTCCAGGGGAGTTCAGTATGCCCTGCTTACATTGAATTCGCTTTGTTCTTACAGCGAAAGAATGCGTGTGGATGCGAAAAATGAAGGGGCTTTGGCAATTTGTGTAAGTTTGGTTGAAGATGACAATGAAAGGATTAGAAAAAATGCTTCTAATTTTGTTCAGGTTCTCAGTGGGAACCATTCCATGAGCTGA

Protein Analysis

383

Amino Acids

41.87

Weight (kDa)

8.06

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 6 - 72 8.8e-18 U-box domain
zf-Nse PF11789 7 - 48 1.4e-06 Zinc-finger of the MIZ type in Nse subunit
zf-RING_UBOX PF13445 11 - 47 3.5e-07 RING-type zinc-finger
ARM_PUB PF25598 118 - 378 1.1e-23 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 137 - 278 8.1e-07 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012930)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21350
fragaria_vesca FvH4_3g03220
malus_domestica MD05G1334100.v1.1 MD10G1309000.v1.1
prunus_persica Prupe.4G031100_v2.0.a1 Prupe.4G031100_v2.0.a1
pyrus_communis pycom05g30580 pycom10g26040
rosa_chinensis RchiOBHm_Chr5g0004861
rosa_laevigata RLG00000031267
rosa_multiflora Rmu_sc0010871.1_g000001
rosa_roxburghii Rroxscaffold_1G00070760
rosa_rugosa Rorug04G0414800
rosa_samantha Rh5AG042300 Rh5BG042000 Rh5CG045800 Rh5DG042400
rosa_wichuraiana Rw5G004090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
Acc36I ACCTGC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 431
AccBSI CCGCTC 1 cut(s) 157
AccII CGCG 3 cut(s) 396, 411, 606
AclWI GGATC 1 cut(s) 814
AcoI YGGCCR 1 cut(s) 124
AcsI RAATTY 1 cut(s) 991
AcyI GRCGYC 3 cut(s) 120, 399, 432
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 6 cut(s) 93, 481, 487, 610, 666, 847
AgsI TTSAA 4 cut(s) 28, 857, 991, 1073
AjnI CCWGG 2 cut(s) 480, 959
AluBI AGCT 8 cut(s) 338, 358, 376, 418, 516, 633, 957, 1146
AluI AGCT 8 cut(s) 338, 358, 376, 418, 516, 633, 957, 1146
Alw21I GWGCWC 2 cut(s) 582, 959
Alw26I GTCTC 1 cut(s) 322
AlwI GGATC 1 cut(s) 814
Ama87I CYCGRG 1 cut(s) 347
AoxI GGCC 5 cut(s) 96, 124, 382, 657, 838
ApeKI GCWGC 3 cut(s) 198, 289, 502
ApoI RAATTY 1 cut(s) 991
AspLEI GCGC 6 cut(s) 294, 396, 413, 434, 608, 751
AspS9I GGNCC 3 cut(s) 96, 591, 838
AsuC2I CCSGG 2 cut(s) 17, 62
AsuHPI GGTGA 2 cut(s) 269, 412
AvaI CYCGRG 1 cut(s) 347
AvaII GGWCC 1 cut(s) 591
BaeGI GKGCMC 1 cut(s) 35
BaeI ACNNNNGTAYC 2 cut(s) 233, 266
BanI GGYRCC 1 cut(s) 431
BanII GRGCYC 3 cut(s) 474, 694, 959
Bbv12I GWGCWC 2 cut(s) 582, 959
BbvI GCAGC 3 cut(s) 210, 301, 489
BccI CCATC 4 cut(s) 110, 282, 767, 895
BceAI ACGGC 5 cut(s) 419, 428, 593, 730, 762
BciT130I CCWGG 2 cut(s) 482, 961
BcnI CCSGG 2 cut(s) 17, 62
BcoDI GTCTC 1 cut(s) 322
BfaI CTAG 1 cut(s) 630
BfoI RGCGCY 3 cut(s) 295, 435, 752
BfuAI ACCTGC 1 cut(s) 143
BglI GCCNNNNNGGC 1 cut(s) 614
BisI GCNGC 9 cut(s) 155, 199, 290, 402, 503, 604, 615, 741, 785
BlsI GCNGC 9 cut(s) 156, 200, 291, 403, 504, 605, 616, 742, 786
Bme1390I CCNGG 4 cut(s) 17, 62, 482, 961
Bme18I GGWCC 1 cut(s) 591
BmeT110I CYCGRG 1 cut(s) 347
BmgT120I GGNCC 3 cut(s) 96, 591, 838
BmiI GGNNCC 5 cut(s) 433, 471, 592, 593, 1133
BmrFI CCNGG 4 cut(s) 17, 62, 482, 961
BmrI ACTGGG 1 cut(s) 308
BmsI GCATC 1 cut(s) 1018
BmuI ACTGGG 1 cut(s) 308
BpmI CTGGAG 2 cut(s) 464, 943
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 2 cut(s) 17, 62
BsaHI GRCGYC 3 cut(s) 120, 399, 432
BsaJI CCNNGG 4 cut(s) 549, 583, 609, 960
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 6 cut(s) 93, 481, 487, 610, 666, 847
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 3 cut(s) 247, 314, 385
BseBI CCWGG 2 cut(s) 482, 961
BseDI CCNNGG 4 cut(s) 549, 583, 609, 960
BseGI GGATG 3 cut(s) 102, 923, 1033
BseLI CCNNNNNNNGG 6 cut(s) 93, 481, 487, 610, 666, 847
BseMII CTCAG 3 cut(s) 23, 721, 1138
BseNI ACTGG 3 cut(s) 247, 314, 385
BseRI GAGGAG 1 cut(s) 482
BseSI GKGCMC 1 cut(s) 35
BseXI GCAGC 3 cut(s) 210, 301, 489
BseYI CCCAGC 2 cut(s) 300, 334
Bsh1236I CGCG 3 cut(s) 396, 411, 606
Bsh1285I CGRYCG 1 cut(s) 21
BshFI GGCC 5 cut(s) 98, 126, 384, 659, 840
BshNI GGYRCC 1 cut(s) 431
BsiEI CGRYCG 1 cut(s) 21
BsiHKAI GWGCWC 2 cut(s) 582, 959
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 4 cut(s) 17, 62, 123, 429
BslFI GGGAC 2 cut(s) 577, 725
BslI CCNNNNNNNGG 6 cut(s) 93, 481, 487, 610, 666, 847
BsmAI GTCTC 1 cut(s) 322
BsmFI GGGAC 2 cut(s) 577, 725
BsmI GAATGC 1 cut(s) 1023
BsnI GGCC 5 cut(s) 98, 126, 384, 659, 840
BsoBI CYCGRG 1 cut(s) 347
Bsp1286I GDGCHC 5 cut(s) 35, 474, 582, 694, 959
Bsp143I GATC 2 cut(s) 91, 819
BspANI GGCC 5 cut(s) 98, 126, 384, 659, 840
BspCNI CTCAG 3 cut(s) 22, 720, 1137
BspFNI CGCG 3 cut(s) 396, 411, 606
BspLI GGNNCC 5 cut(s) 433, 471, 592, 593, 1133
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 1 cut(s) 814
BspT107I GGYRCC 1 cut(s) 431
BsrBI CCGCTC 1 cut(s) 157
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 3 cut(s) 247, 314, 385
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 4 cut(s) 549, 583, 609, 960
BssMI GATC 2 cut(s) 91, 819
BssNI GRCGYC 3 cut(s) 120, 399, 432
BssT1I CCWWGG 1 cut(s) 583
Bst2UI CCWGG 2 cut(s) 482, 961
Bst6I CTCTTC 2 cut(s) 79, 891
BstACI GRCGYC 3 cut(s) 120, 399, 432
BstC8I GCNNGC 4 cut(s) 113, 124, 287, 789
BstDEI CTNAG 3 cut(s) 9, 707, 1124
BstDSI CCRYGG 1 cut(s) 609
BstF5I GGATG 3 cut(s) 102, 923, 1033
BstFNI CGCG 3 cut(s) 396, 411, 606
BstH2I RGCGCY 3 cut(s) 295, 435, 752
BstHHI GCGC 6 cut(s) 294, 396, 413, 434, 608, 751
BstKTI GATC 2 cut(s) 94, 822
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 2 cut(s) 91, 819
BstMCI CGRYCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 9 cut(s) 291, 364, 373, 410, 440, 554, 614, 689, 746
BstNI CCWGG 2 cut(s) 482, 961
BstSCI CCNGG 4 cut(s) 15, 60, 480, 959
BstSLI GKGCMC 1 cut(s) 35
BstUI CGCG 3 cut(s) 396, 411, 606
BstV1I GCAGC 3 cut(s) 210, 301, 489
BstXI CCANNNNNNTGG 1 cut(s) 380
BsuRI GGCC 5 cut(s) 98, 126, 384, 659, 840
BtgI CCRYGG 1 cut(s) 609
BtsCI GGATG 3 cut(s) 102, 923, 1033
BtsIMutI CAGTG 2 cut(s) 378, 1132
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 4 cut(s) 113, 124, 287, 789
CfoI GCGC 6 cut(s) 294, 396, 413, 434, 608, 751
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 3 cut(s) 96, 591, 838
CseI GACGC 3 cut(s) 128, 331, 407
Csp6I GTAC 1 cut(s) 250
CviAII CATG 1 cut(s) 1141
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 3 cut(s) 9, 707, 1124
DinI GGCGCC 1 cut(s) 433
DpnI GATC 2 cut(s) 93, 821
DpnII GATC 2 cut(s) 91, 819
EaeI YGGCCR 1 cut(s) 124
Eam1104I CTCTTC 2 cut(s) 79, 891
EarI CTCTTC 2 cut(s) 79, 891
EciI GGCGGA 1 cut(s) 430
Ecl136II GAGCTC 1 cut(s) 957
Eco130I CCWWGG 1 cut(s) 583
Eco24I GRGCYC 3 cut(s) 474, 694, 959
Eco47I GGWCC 1 cut(s) 591
Eco53kI GAGCTC 1 cut(s) 957
Eco88I CYCGRG 1 cut(s) 347
EcoICRI GAGCTC 1 cut(s) 957
EcoO109I RGGNCCY 1 cut(s) 591
EcoRI GAATTC 1 cut(s) 991
EcoRII CCWGG 2 cut(s) 480, 959
EcoT14I CCWWGG 1 cut(s) 583
EcoT38I GRGCYC 3 cut(s) 474, 694, 959
EgeI GGCGCC 1 cut(s) 433
EheI GGCGCC 1 cut(s) 433
ErhI CCWWGG 1 cut(s) 583
FaeI CATG 1 cut(s) 1144
FaiI YATR 7 cut(s) 53, 68, 216, 673, 681, 975, 1142
FalI AAGNNNNNCTT 2 cut(s) 525, 557
FaqI GGGAC 2 cut(s) 577, 725
FatI CATG 1 cut(s) 1140
FauI CCCGC 2 cut(s) 482, 780
Fnu4HI GCNGC 9 cut(s) 155, 199, 290, 402, 503, 604, 615, 741, 785
FokI GGATG 3 cut(s) 89, 930, 1040
FriOI GRGCYC 3 cut(s) 474, 694, 959
Fsp4HI GCNGC 9 cut(s) 155, 199, 290, 402, 503, 604, 615, 741, 785
FspBI CTAG 1 cut(s) 630
GlaI GCGC 6 cut(s) 293, 395, 412, 433, 607, 750
GluI GCNGC 9 cut(s) 155, 199, 290, 402, 503, 604, 615, 741, 785
GsaI CCCAGC 2 cut(s) 304, 338
GsuI CTGGAG 2 cut(s) 464, 943
HaeII RGCGCY 3 cut(s) 295, 435, 752
HaeIII GGCC 5 cut(s) 98, 126, 384, 659, 840
HapII CCGG 4 cut(s) 17, 62, 123, 429
HgaI GACGC 3 cut(s) 128, 331, 407
HhaI GCGC 6 cut(s) 294, 396, 413, 434, 608, 751
Hin1I GRCGYC 3 cut(s) 120, 399, 432
Hin1II CATG 1 cut(s) 1144
Hin6I GCGC 6 cut(s) 292, 394, 411, 432, 606, 749
HinP1I GCGC 6 cut(s) 292, 394, 411, 432, 606, 749
HincII GTYRAC 3 cut(s) 460, 627, 652
HindII GTYRAC 3 cut(s) 460, 627, 652
HinfI GANTC 8 cut(s) 7, 350, 362, 425, 461, 567, 721, 833
HpaII CCGG 4 cut(s) 17, 62, 123, 429
HphI GGTGA 2 cut(s) 269, 412
Hpy166II GTNNAC 3 cut(s) 460, 627, 652
Hpy188I TCNGA 4 cut(s) 58, 466, 495, 824
Hpy188III TCNNGA 2 cut(s) 319, 725
Hpy8I GTNNAC 3 cut(s) 460, 627, 652
Hpy99I CGWCG 5 cut(s) 122, 325, 401, 527, 622
HpyAV CCTTC 5 cut(s) 180, 230, 415, 881, 1034
HpyCH4V TGCA 2 cut(s) 683, 882
HpyF10VI GCNNNNNNNGC 9 cut(s) 291, 364, 373, 410, 440, 554, 614, 689, 746
HpyF3I CTNAG 3 cut(s) 9, 707, 1124
Hsp92I GRCGYC 3 cut(s) 120, 399, 432
Hsp92II CATG 1 cut(s) 1144
HspAI GCGC 6 cut(s) 292, 394, 411, 432, 606, 749
KasI GGCGCC 1 cut(s) 431
KflI GGGWCCC 1 cut(s) 591
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Kzo9I GATC 2 cut(s) 91, 819
LmnI GCTCC 6 cut(s) 162, 469, 554, 585, 954, 962
Lsp1109I GCAGC 3 cut(s) 210, 301, 489
LweI GCATC 1 cut(s) 1018
MaeI CTAG 1 cut(s) 630
MaeIII GTNAC 1 cut(s) 80
MalI GATC 2 cut(s) 93, 821
MbiI CCGCTC 1 cut(s) 157
MboI GATC 2 cut(s) 91, 819
MboII GAAGA 3 cut(s) 66, 908, 1085
MhlI GDGCHC 5 cut(s) 35, 474, 582, 694, 959
MluCI AATT 8 cut(s) 48, 141, 236, 684, 825, 991, 1053, 1108
Mly113I GGCGCC 1 cut(s) 432
MlyI GAGTC 5 cut(s) 356, 359, 434, 455, 730
MmeI TCCRAC 2 cut(s) 81, 130
MroNI GCCGGC 1 cut(s) 122
MseI TTAA 1 cut(s) 912
MspI CCGG 4 cut(s) 17, 62, 123, 429
MspR9I CCNGG 4 cut(s) 17, 62, 482, 961
Mva1269I GAATGC 1 cut(s) 1023
MvaI CCWGG 2 cut(s) 482, 961
MvnI CGCG 3 cut(s) 396, 411, 606
MwoI GCNNNNNNNGC 9 cut(s) 291, 364, 373, 410, 440, 554, 614, 689, 746
NaeI GCCGGC 1 cut(s) 124
NarI GGCGCC 1 cut(s) 432
NciI CCSGG 2 cut(s) 17, 62
NdeII GATC 2 cut(s) 91, 819
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 1 cut(s) 1144
NlaIV GGNNCC 5 cut(s) 433, 471, 592, 593, 1133
NmeAIII GCCGAG 1 cut(s) 574
NmuCI GTSAC 1 cut(s) 80
PaeR7I CTCGAG 1 cut(s) 347
PaqCI CACCTGC 1 cut(s) 143
PcsI WCGNNNNNNNCGW 2 cut(s) 366, 567
PctI GAATGC 1 cut(s) 1023
PdiI GCCGGC 1 cut(s) 124
PfeI GAWTC 2 cut(s) 567, 833
PfoI TCCNGGA 1 cut(s) 480
PkrI GCNGC 9 cut(s) 156, 200, 291, 403, 504, 605, 616, 742, 786
PleI GAGTC 5 cut(s) 356, 358, 433, 455, 729
PluTI GGCGCC 1 cut(s) 435
PpsI GAGTC 5 cut(s) 356, 358, 433, 455, 729
PpuMI RGGWCCY 1 cut(s) 591
Psp124BI GAGCTC 1 cut(s) 959
Psp5II RGGWCCY 1 cut(s) 591
Psp6I CCWGG 2 cut(s) 480, 959
PspFI CCCAGC 2 cut(s) 300, 334
PspGI CCWGG 2 cut(s) 480, 959
PspN4I GGNNCC 5 cut(s) 433, 471, 592, 593, 1133
PspPI GGNCC 3 cut(s) 96, 591, 838
PspPPI RGGWCCY 1 cut(s) 591
PspXI VCTCGAGB 1 cut(s) 347
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SacI GAGCTC 1 cut(s) 959
SaqAI TTAA 1 cut(s) 912
SatI GCNGC 9 cut(s) 155, 199, 290, 402, 503, 604, 615, 741, 785
Sau3AI GATC 2 cut(s) 91, 819
Sau96I GGNCC 3 cut(s) 96, 591, 838
SchI GAGTC 5 cut(s) 356, 359, 434, 455, 730
ScrFI CCNGG 4 cut(s) 17, 62, 482, 961
SduI GDGCHC 5 cut(s) 35, 474, 582, 694, 959
SfaNI GCATC 1 cut(s) 1018
SfoI GGCGCC 1 cut(s) 433
Sfr274I CTCGAG 1 cut(s) 347
SinI GGWCC 1 cut(s) 591
SlaI CTCGAG 1 cut(s) 347
SmlI CTYRAG 2 cut(s) 347, 449
SmoI CTYRAG 2 cut(s) 347, 449
Sse9I AATT 8 cut(s) 48, 141, 236, 684, 825, 991, 1053, 1108
SspDI GGCGCC 1 cut(s) 431
SspMI CTAG 1 cut(s) 630
SstI GAGCTC 1 cut(s) 959
StyD4I CCNGG 4 cut(s) 15, 60, 480, 959
StyI CCWWGG 1 cut(s) 583
TaqII GACCGA 1 cut(s) 854
TasI AATT 8 cut(s) 48, 141, 236, 684, 825, 991, 1053, 1108
TauI GCSGC 6 cut(s) 157, 404, 606, 617, 743, 787
TfiI GAWTC 2 cut(s) 567, 833
Tru1I TTAA 1 cut(s) 912
Tru9I TTAA 1 cut(s) 912
TscAI CASTG 2 cut(s) 385, 1132
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 3 cut(s) 198, 289, 502
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 2 cut(s) 1053, 1098
TspRI CASTG 2 cut(s) 385, 1132
VpaK11BI GGWCC 1 cut(s) 591
XapI RAATTY 1 cut(s) 991
XhoI CTCGAG 1 cut(s) 347
XspI CTAG 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.