Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0012930

Physical Location & Seq

Genomic Coordinates Forward (+)

30653492 .. 30656123

Gene Structure 2632 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g30580.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1203 bp

ATGGCGACTCAGTTCCCGGACGATTTCAAGTGCCCGATTTCCCTCGAAATAATGTCCGACCCTGTTATACTCTCCTCGGGTCACACCTTTGATCGGGCCTCCATCCAACGCTGGCTCGACGCCGGCCACCGTACCTGCCCAATCACCAAACTGCCCCTCCCCGAACACCCTTCCCTCATCCCCAACCACGCCCTCCGCAGCCTGATTTCCACTTATACCCTTCTCTCCCCTGCCAAATTACAACACCAGTATCACCACCACCACCACCAATACCATCACCACTGCCAGCAGCGCCACTCCCAGCCCCAAAACCCAATATCGACGCTTACCTCTCCAGCTTCCACCCTCGAGTCCAAGCTCGAATCGCTCGACCAGCTCACTCGCCTCTCCAAGCGCGACGCCGCCTTTCGCGCCAAGCTCACTGAGTCCGGCGCCGTCTCCACCGTCCTCAAGTGCGTCGACTCGGAGGAGCCCCGCCTCCAAGAAAAGGCGCTGACTTTGCTGCTCAATGTCAGCTTGGACGACGATAACAAAGTGGGTCTTGTCGCCGAGGGAGCAATCGCTCGAATCGTGGAGGTGCTCCAAGGTGGGTCCCCCAACAGCCGTGCCGTGGCAGCGACGATGTTGACGAGCTTGGCAGTGGTGGAGGTCAACAAGGCCACAATCGGGGCATACCCGTGTGCAATTCGGGCTCTAGTTTCGCTTCTCGGGGACGGCAAAAGTCGAGAGCAGAAAGAGGCGGCCACGGCGCTTTACACCATCTGTTTGTTCCCGGATAACCGGCGGCGGGCTGTGGAATGTGGGGTGGTGTCGATTTTGATCCGAATTGCTGAATCGGGCCTCGAACGGGCGGTTGAAGTTCTGGGTCTTTTGGCAAAGTGCAAGGAAGGTAGAGAAGAGATGGAGAGGTTTAATGGTTGTGTGGGGATTTTAGTGAGGGTTTTAGAAAATGGGAGCTCCAGGGGAGTTCAGTATGCCCTGCTTACTTTGAATTTGCTTTGTTCTTATAGCGAACAAATGCGTGTGGAAGCGAAAAATGAAGGGGCTTTGGCAATTTGTGTAGGTTTGGTTGAAGATGATAATGAAAGGATTGGAAAAAATGCTTCTAATTTGGTTCAGATTCTCAGTGGGAACCAATCCATGAGCGTGAGGGGAGTTTGCTCAAGTAATTCATATCTTTCATGTGAGTCGCTAGCAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0019538 GO:0032446 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0070647 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

U-box

Protein Analysis

401

Amino Acids

43.61

Weight (kDa)

6.7

Isoelectric Point (pI)

43.7

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012930)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21350
fragaria_vesca	FvH4_3g03220
malus_domestica	MD05G1334100.v1.1 MD10G1309000.v1.1
prunus_persica	Prupe.4G031100_v2.0.a1 Prupe.4G031100_v2.0.a1
pyrus_communis	pycom05g30580 pycom10g26040
rosa_chinensis	RchiOBHm_Chr5g0004861
rosa_laevigata	RLG00000031267
rosa_multiflora	Rmu_sc0010871.1_g000001
rosa_roxburghii	Rroxscaffold_1G00070760
rosa_rugosa	Rorug04G0414800
rosa_samantha	Rh5AG042300 Rh5BG042000 Rh5CG045800 Rh5DG042400
rosa_wichuraiana	Rw5G004090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	143
AccB1I	GGYRCC	1 cut(s)	431
AccI	GTMKAC	1 cut(s)	459
AccII	CGCG	2 cut(s)	396, 411
AciI	CCGC	7 cut(s)	196, 402, 475, 740, 784, 787, 851
AclWI	GGATC	1 cut(s)	814
AcoI	YGGCCR	2 cut(s)	124, 741
AcsI	RAATTY	1 cut(s)	991
AcyI	GRCGYC	3 cut(s)	120, 399, 432
AfaI	GTAC	1 cut(s)	133
AfiI	CCNNNNNNNGG	6 cut(s)	93, 487, 610, 666, 787, 847
AgsI	TTSAA	4 cut(s)	28, 857, 991, 1073
AjnI	CCWGG	1 cut(s)	959
AluBI	AGCT	7 cut(s)	338, 358, 376, 418, 516, 633, 957
AluI	AGCT	7 cut(s)	338, 358, 376, 418, 516, 633, 957
Alw21I	GWGCWC	2 cut(s)	582, 959
Alw26I	GTCTC	1 cut(s)	442
AlwI	GGATC	1 cut(s)	814
Ama87I	CYCGRG	3 cut(s)	76, 347, 707
AoxI	GGCC	5 cut(s)	96, 124, 657, 741, 838
ApeKI	GCWGC	4 cut(s)	198, 289, 502, 614
ApoI	RAATTY	1 cut(s)	991
AspLEI	GCGC	6 cut(s)	294, 396, 413, 434, 493, 751
AspS9I	GGNCC	3 cut(s)	96, 591, 838
AsuC2I	CCSGG	2 cut(s)	17, 773
AsuHPI	GGTGA	3 cut(s)	136, 245, 269
AsuNHI	GCTAGC	1 cut(s)	1192
AvaI	CYCGRG	3 cut(s)	76, 347, 707
AvaII	GGWCC	1 cut(s)	591
BaeGI	GKGCMC	1 cut(s)	35
BaeI	ACNNNNGTAYC	2 cut(s)	233, 266
BanI	GGYRCC	1 cut(s)	431
BanII	GRGCYC	3 cut(s)	474, 694, 959
Bbv12I	GWGCWC	2 cut(s)	582, 959
BbvI	GCAGC	4 cut(s)	210, 301, 489, 626
BccI	CCATC	4 cut(s)	110, 282, 767, 895
BceAI	ACGGC	5 cut(s)	419, 588, 593, 730, 762
BciT130I	CCWGG	1 cut(s)	961
BcnI	CCSGG	2 cut(s)	17, 773
BcoDI	GTCTC	1 cut(s)	442
BfaI	CTAG	2 cut(s)	695, 1193
BfoI	RGCGCY	4 cut(s)	295, 435, 494, 752
BfuAI	ACCTGC	1 cut(s)	143
BisI	GCNGC	7 cut(s)	199, 290, 402, 503, 615, 741, 785
BlsI	GCNGC	7 cut(s)	200, 291, 403, 504, 616, 742, 786
Bme1390I	CCNGG	3 cut(s)	17, 773, 961
Bme18I	GGWCC	1 cut(s)	591
BmeT110I	CYCGRG	3 cut(s)	76, 347, 707
BmgT120I	GGNCC	3 cut(s)	96, 591, 838
BmiI	GGNNCC	5 cut(s)	433, 471, 592, 593, 1133
BmrFI	CCNGG	3 cut(s)	17, 773, 961
BmtI	GCTAGC	1 cut(s)	1196
BpmI	CTGGAG	2 cut(s)	318, 943
BpuEI	CTTGAG	2 cut(s)	434, 1147
BpuMI	CCSGG	2 cut(s)	17, 773
BsaBI	GATNNNNATC	1 cut(s)	818
BsaHI	GRCGYC	3 cut(s)	120, 399, 432
BsaJI	CCNNGG	6 cut(s)	75, 549, 583, 609, 744, 960
BsaXI	ACNNNNNCTCC	2 cut(s)	141, 171
Bsc4I	CCNNNNNNNGG	6 cut(s)	93, 487, 610, 666, 787, 847
Bse118I	RCCGGY	2 cut(s)	122, 780
Bse1I	ACTGG	1 cut(s)	247
Bse8I	GATNNNNATC	1 cut(s)	818
BseBI	CCWGG	1 cut(s)	961
BseDI	CCNNGG	6 cut(s)	75, 549, 583, 609, 744, 960
BseGI	GGATG	2 cut(s)	102, 177
BseJI	GATNNNNATC	1 cut(s)	818
BseLI	CCNNNNNNNGG	6 cut(s)	93, 487, 610, 666, 787, 847
BseMII	CTCAG	3 cut(s)	23, 414, 1138
BseNI	ACTGG	1 cut(s)	247
BseRI	GAGGAG	2 cut(s)	64, 482
BseSI	GKGCMC	1 cut(s)	35
BseXI	GCAGC	4 cut(s)	210, 301, 489, 626
BseYI	CCCAGC	1 cut(s)	300
Bsh1236I	CGCG	2 cut(s)	396, 411
BshFI	GGCC	5 cut(s)	98, 126, 659, 743, 840
BshNI	GGYRCC	1 cut(s)	431
BsiHKAI	GWGCWC	2 cut(s)	582, 959
BsiHKCI	CYCGRG	3 cut(s)	76, 347, 707
BsiSI	CCGG	5 cut(s)	17, 123, 429, 773, 781
BslFI	GGGAC	2 cut(s)	577, 725
BslI	CCNNNNNNNGG	6 cut(s)	93, 487, 610, 666, 787, 847
BsmAI	GTCTC	1 cut(s)	442
BsmBI	CGTCTC	1 cut(s)	442
BsmFI	GGGAC	2 cut(s)	577, 725
BsnI	GGCC	5 cut(s)	98, 126, 659, 743, 840
BsoBI	CYCGRG	3 cut(s)	76, 347, 707
Bsp1286I	GDGCHC	5 cut(s)	35, 474, 582, 694, 959
Bsp143I	GATC	2 cut(s)	91, 819
BspACI	CCGC	7 cut(s)	196, 402, 475, 740, 784, 787, 851
BspANI	GGCC	5 cut(s)	98, 126, 659, 743, 840
BspCNI	CTCAG	3 cut(s)	22, 415, 1137
BspFNI	CGCG	2 cut(s)	396, 411
BspLI	GGNNCC	5 cut(s)	433, 471, 592, 593, 1133
BspMI	ACCTGC	1 cut(s)	143
BspOI	GCTAGC	1 cut(s)	1196
BspPI	GGATC	1 cut(s)	814
BspT107I	GGYRCC	1 cut(s)	431
BsrFI	RCCGGY	2 cut(s)	122, 780
BsrI	ACTGG	1 cut(s)	247
BssAI	RCCGGY	2 cut(s)	122, 780
BssECI	CCNNGG	6 cut(s)	75, 549, 583, 609, 744, 960
BssMI	GATC	2 cut(s)	91, 819
BssNI	GRCGYC	3 cut(s)	120, 399, 432
BssT1I	CCWWGG	1 cut(s)	583
Bst2UI	CCWGG	1 cut(s)	961
Bst4CI	ACNGT	2 cut(s)	131, 445
Bst6I	CTCTTC	1 cut(s)	891
BstACI	GRCGYC	3 cut(s)	120, 399, 432
BstC8I	GCNNGC	5 cut(s)	113, 124, 287, 789, 1194
BstDEI	CTNAG	3 cut(s)	9, 423, 1124
BstDSI	CCRYGG	2 cut(s)	609, 744
BstF5I	GGATG	2 cut(s)	102, 177
BstFNI	CGCG	2 cut(s)	396, 411
BstH2I	RGCGCY	4 cut(s)	295, 435, 494, 752
BstHHI	GCGC	6 cut(s)	294, 396, 413, 434, 493, 751
BstKTI	GATC	2 cut(s)	94, 822
BstMAI	GTCTC	1 cut(s)	442
BstMBI	GATC	2 cut(s)	91, 819
BstMWI	GCNNNNNNNGC	9 cut(s)	291, 364, 373, 410, 499, 554, 614, 689, 746
BstNI	CCWGG	1 cut(s)	961
BstSCI	CCNGG	3 cut(s)	15, 771, 959
BstSLI	GKGCMC	1 cut(s)	35
BstUI	CGCG	2 cut(s)	396, 411
BstV1I	GCAGC	4 cut(s)	210, 301, 489, 626
BsuRI	GGCC	5 cut(s)	98, 126, 659, 743, 840
BtgI	CCRYGG	2 cut(s)	609, 744
BtsCI	GGATG	2 cut(s)	102, 177
BtsI	GCAGTG	2 cut(s)	280, 645
BtsIMutI	CAGTG	4 cut(s)	280, 420, 645, 1132
BveI	ACCTGC	1 cut(s)	143
Cac8I	GCNNGC	5 cut(s)	113, 124, 287, 789, 1194
CfoI	GCGC	6 cut(s)	294, 396, 413, 434, 493, 751
Cfr10I	RCCGGY	2 cut(s)	122, 780
Cfr13I	GGNCC	3 cut(s)	96, 591, 838
CseI	GACGC	4 cut(s)	128, 331, 407, 445
Csp6I	GTAC	1 cut(s)	132
CviAII	CATG	2 cut(s)	1141, 1182
CviQI	GTAC	1 cut(s)	132
DdeI	CTNAG	3 cut(s)	9, 423, 1124
DinI	GGCGCC	1 cut(s)	433
DpnI	GATC	2 cut(s)	93, 821
DpnII	GATC	2 cut(s)	91, 819
EaeI	YGGCCR	2 cut(s)	124, 741
Eam1104I	CTCTTC	1 cut(s)	891
EarI	CTCTTC	1 cut(s)	891
Ecl136II	GAGCTC	1 cut(s)	957
Eco130I	CCWWGG	1 cut(s)	583
Eco24I	GRGCYC	3 cut(s)	474, 694, 959
Eco47I	GGWCC	1 cut(s)	591
Eco53kI	GAGCTC	1 cut(s)	957
Eco88I	CYCGRG	3 cut(s)	76, 347, 707
EcoICRI	GAGCTC	1 cut(s)	957
EcoO109I	RGGNCCY	1 cut(s)	591
EcoRII	CCWGG	1 cut(s)	959
EcoT14I	CCWWGG	1 cut(s)	583
EcoT38I	GRGCYC	3 cut(s)	474, 694, 959
EgeI	GGCGCC	1 cut(s)	433
EheI	GGCGCC	1 cut(s)	433
ErhI	CCWWGG	1 cut(s)	583
Esp3I	CGTCTC	1 cut(s)	442
FaeI	CATG	2 cut(s)	1144, 1185
FaiI	YATR	8 cut(s)	68, 216, 673, 975, 1008, 1142, 1174, 1183
FalI	AAGNNNNNCTT	2 cut(s)	525, 557
FaqI	GGGAC	2 cut(s)	577, 725
FatI	CATG	2 cut(s)	1140, 1181
FauI	CCCGC	2 cut(s)	482, 780
FblI	GTMKAC	1 cut(s)	459
Fnu4HI	GCNGC	7 cut(s)	199, 290, 402, 503, 615, 741, 785
FokI	GGATG	2 cut(s)	89, 164
FriOI	GRGCYC	3 cut(s)	474, 694, 959
Fsp4HI	GCNGC	7 cut(s)	199, 290, 402, 503, 615, 741, 785
FspBI	CTAG	2 cut(s)	695, 1193
GlaI	GCGC	6 cut(s)	293, 395, 412, 433, 492, 750
GluI	GCNGC	7 cut(s)	199, 290, 402, 503, 615, 741, 785
GsaI	CCCAGC	1 cut(s)	304
GsuI	CTGGAG	2 cut(s)	318, 943
HaeII	RGCGCY	4 cut(s)	295, 435, 494, 752
HaeIII	GGCC	5 cut(s)	98, 126, 659, 743, 840
HapII	CCGG	5 cut(s)	17, 123, 429, 773, 781
HgaI	GACGC	4 cut(s)	128, 331, 407, 445
HhaI	GCGC	6 cut(s)	294, 396, 413, 434, 493, 751
Hin1I	GRCGYC	3 cut(s)	120, 399, 432
Hin1II	CATG	2 cut(s)	1144, 1185
Hin6I	GCGC	6 cut(s)	292, 394, 411, 432, 491, 749
HinP1I	GCGC	6 cut(s)	292, 394, 411, 432, 491, 749
HincII	GTYRAC	3 cut(s)	460, 627, 652
HindII	GTYRAC	3 cut(s)	460, 627, 652
HinfI	GANTC	9 cut(s)	7, 350, 362, 425, 461, 567, 833, 1120, 1187
HpaII	CCGG	5 cut(s)	17, 123, 429, 773, 781
HphI	GGTGA	3 cut(s)	136, 245, 269
Hpy166II	GTNNAC	3 cut(s)	460, 627, 652
Hpy188I	TCNGA	4 cut(s)	58, 466, 824, 1119
Hpy188III	TCNNGA	1 cut(s)	725
Hpy8I	GTNNAC	3 cut(s)	460, 627, 652
Hpy99I	CGWCG	6 cut(s)	122, 325, 401, 461, 527, 622
HpyAV	CCTTC	4 cut(s)	180, 230, 881, 1034
HpyCH4III	ACNGT	2 cut(s)	131, 445
HpyCH4V	TGCA	2 cut(s)	683, 882
HpyF10VI	GCNNNNNNNGC	9 cut(s)	291, 364, 373, 410, 499, 554, 614, 689, 746
HpyF3I	CTNAG	3 cut(s)	9, 423, 1124
Hsp92I	GRCGYC	3 cut(s)	120, 399, 432
Hsp92II	CATG	2 cut(s)	1144, 1185
HspAI	GCGC	6 cut(s)	292, 394, 411, 432, 491, 749
KasI	GGCGCC	1 cut(s)	431
KflI	GGGWCCC	1 cut(s)	591
KroI	GCCGGC	1 cut(s)	122
KroNI	GCCGGC	1 cut(s)	124
Kzo9I	GATC	2 cut(s)	91, 819
LmnI	GCTCC	5 cut(s)	469, 554, 585, 954, 962
Lsp1109I	GCAGC	4 cut(s)	210, 301, 489, 626
MaeI	CTAG	2 cut(s)	695, 1193
MaeIII	GTNAC	1 cut(s)	80
MalI	GATC	2 cut(s)	93, 821
MboI	GATC	2 cut(s)	91, 819
MboII	GAAGA	2 cut(s)	908, 1085
MhlI	GDGCHC	5 cut(s)	35, 474, 582, 694, 959
MluCI	AATT	7 cut(s)	236, 684, 825, 991, 1053, 1108, 1168
Mly113I	GGCGCC	1 cut(s)	432
MlyI	GAGTC	4 cut(s)	359, 434, 455, 1196
MmeI	TCCRAC	2 cut(s)	81, 130
MroNI	GCCGGC	1 cut(s)	122
MseI	TTAA	2 cut(s)	912, 1201
MslI	CAYNNNNRTG	2 cut(s)	676, 1145
MspI	CCGG	5 cut(s)	17, 123, 429, 773, 781
MspR9I	CCNGG	3 cut(s)	17, 773, 961
MvaI	CCWGG	1 cut(s)	961
MvnI	CGCG	2 cut(s)	396, 411
MwoI	GCNNNNNNNGC	9 cut(s)	291, 364, 373, 410, 499, 554, 614, 689, 746
NaeI	GCCGGC	1 cut(s)	124
NarI	GGCGCC	1 cut(s)	432
NciI	CCSGG	2 cut(s)	17, 773
NdeII	GATC	2 cut(s)	91, 819
NgoMIV	GCCGGC	1 cut(s)	122
NheI	GCTAGC	1 cut(s)	1192
NlaIII	CATG	2 cut(s)	1144, 1185
NlaIV	GGNNCC	5 cut(s)	433, 471, 592, 593, 1133
NmeAIII	GCCGAG	1 cut(s)	574
NmuCI	GTSAC	1 cut(s)	80
PaeR7I	CTCGAG	1 cut(s)	347
PcsI	WCGNNNNNNNCGW	3 cut(s)	366, 567, 626
PdiI	GCCGGC	1 cut(s)	124
PfeI	GAWTC	4 cut(s)	362, 567, 833, 1120
PfoI	TCCNGGA	2 cut(s)	15, 771
PkrI	GCNGC	7 cut(s)	200, 291, 403, 504, 616, 742, 786
PleI	GAGTC	4 cut(s)	358, 433, 455, 1195
PluTI	GGCGCC	1 cut(s)	435
PpsI	GAGTC	4 cut(s)	358, 433, 455, 1195
PpuMI	RGGWCCY	1 cut(s)	591
Psp124BI	GAGCTC	1 cut(s)	959
Psp5II	RGGWCCY	1 cut(s)	591
Psp6I	CCWGG	1 cut(s)	959
PspFI	CCCAGC	1 cut(s)	300
PspGI	CCWGG	1 cut(s)	959
PspN4I	GGNNCC	5 cut(s)	433, 471, 592, 593, 1133
PspPI	GGNCC	3 cut(s)	96, 591, 838
PspPPI	RGGWCCY	1 cut(s)	591
PspXI	VCTCGAGB	1 cut(s)	347
RsaI	GTAC	1 cut(s)	133
RsaNI	GTAC	1 cut(s)	132
RseI	CAYNNNNRTG	2 cut(s)	676, 1145
SacI	GAGCTC	1 cut(s)	959
SalI	GTCGAC	1 cut(s)	458
SaqAI	TTAA	2 cut(s)	912, 1201
SatI	GCNGC	7 cut(s)	199, 290, 402, 503, 615, 741, 785
Sau3AI	GATC	2 cut(s)	91, 819
Sau96I	GGNCC	3 cut(s)	96, 591, 838
SchI	GAGTC	4 cut(s)	359, 434, 455, 1196
ScrFI	CCNGG	3 cut(s)	17, 773, 961
SduI	GDGCHC	5 cut(s)	35, 474, 582, 694, 959
SfoI	GGCGCC	1 cut(s)	433
Sfr274I	CTCGAG	1 cut(s)	347
SinI	GGWCC	1 cut(s)	591
SlaI	CTCGAG	1 cut(s)	347
SmiMI	CAYNNNNRTG	2 cut(s)	676, 1145
SmlI	CTYRAG	3 cut(s)	347, 449, 1162
SmoI	CTYRAG	3 cut(s)	347, 449, 1162
Sse9I	AATT	7 cut(s)	236, 684, 825, 991, 1053, 1108, 1168
SsiI	CCGC	7 cut(s)	196, 402, 475, 740, 784, 787, 851
SspDI	GGCGCC	1 cut(s)	431
SspMI	CTAG	2 cut(s)	695, 1193
SstI	GAGCTC	1 cut(s)	959
StyD4I	CCNGG	3 cut(s)	15, 771, 959
StyI	CCWWGG	1 cut(s)	583
TaaI	ACNGT	2 cut(s)	131, 445
TasI	AATT	7 cut(s)	236, 684, 825, 991, 1053, 1108, 1168
TauI	GCSGC	3 cut(s)	404, 743, 787
TfiI	GAWTC	4 cut(s)	362, 567, 833, 1120
Tru1I	TTAA	2 cut(s)	912, 1201
Tru9I	TTAA	2 cut(s)	912, 1201
TscAI	CASTG	4 cut(s)	287, 427, 645, 1132
TseFI	GTSAC	1 cut(s)	80
TseI	GCWGC	4 cut(s)	198, 289, 502, 614
Tsp45I	GTSAC	1 cut(s)	80
TspDTI	ATGAA	4 cut(s)	1053, 1098, 1161, 1170
TspRI	CASTG	4 cut(s)	287, 427, 645, 1132
VpaK11BI	GGWCC	1 cut(s)	591
XapI	RAATTY	1 cut(s)	991
XhoI	CTCGAG	1 cut(s)	347
XmiI	GTMKAC	1 cut(s)	459
XspI	CTAG	2 cut(s)	695, 1193

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.