MD10G1309000.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012930
Physical Location & Seq
Forward (+)
39377526 .. 39378826
1301 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1309000.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGGCGACTCAGTTCCCGGTCGATTTCAAGTGCCCGATTTCGCTCGAAATTATGTCCGACCCGGTTATACTCTCTTCGGGTCACACCTTTGATCGGGCCTCCATCCAACGCTGGCTCGACGCCGGCCACCGCACCTGCCCAATTTCCAAACTGCCCCTCCCCGACCACCCTTCCCTCATCCCCAACCACTCCCTCCGCAGCCTGATTTCCACTTATACCCTTCTCTCCCCTGCCAAATTACAACACCAGTACCACCAGCACCACCACCAATACCATCACCACCGCCAGCAGCGCCACTCCCAGCCCCAAACCCCTGTCTCGACGCTTACCTCCCCAGCTTCCACCCTCGAGTCCAAGCTCGAGTCGCTCGACCAGCTTACTCGCCTCTCCAAGCGCGACGCTGCCTTCCGCGCCAAGCTGACCGAGTCCGGCGCCGTTTCCGCCGTCCTCAAGTGCGTTGACTCGGAAGAGCCCCGCCTCCAGGAAAAGGCGCTGACTTTGCTGCTCAATGTTAGCTTGGACGACGATAACAAAGTGGGTCTTGTCGCCGAGGGAGCAATCGCTCGAATCGTGGAGGTGCTCCAAGGTGGGTCCCCCAACAGCCGCGCCGTGGCGGCGACGATGTTGACTAGCTTGGCGGTGGTGGAGGTCAACAAGGCCACAATCGGGGCATACCCGTGTGCAATTCGGGCTCTGGTTTCGCTTCTCAGGGACGGCAAGAGTCGAGAGCAGAAGGAGGCGGCGACGGCGCTTTACACCATCTGTTTGTTCCCGGATAACCGGCGGCGGGCGGTGGAATGTGGGGCGGTGTCGTTTTTGATCCGAATTGCTGAATCGGGCCTCGAACGGGCGGTTGAAGTTCTCGGTCTTTTGGCGAAGTGCAAGGAAGGTAGAGAAGAGATGGAGAGGTTTAATGGATGTGTGGGGATTTTAGTGAGGGTTTTAGAGAATGGGAGCTCCAGGGGAGTTCAGTATGCCCTGCTCACATTGAATTCGCTTTGTTCTTACAGCGAACGGATGCGTGTGGAAGCGAAAAATGAAAGGGCTTTATCACTTTGTGTAGGTTTGGTTGAAGATGACAATGAAGGGATTAGAAAAAATTCTTCTAATTTGGTTCAGGTTCTCAGTGGGAACCATTCCATGAGCTGA

Protein Analysis

383

Amino Acids

41.96

Weight (kDa)

8.3

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 6 - 72 1.1e-17 U-box domain
zf-Nse PF11789 7 - 48 1.4e-06 Zinc-finger of the MIZ type in Nse subunit
zf-RING_UBOX PF13445 11 - 47 3.5e-07 RING-type zinc-finger
ARM_PUB PF25598 118 - 344 3.4e-23 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 136 - 278 2.3e-07 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012930)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21350
fragaria_vesca FvH4_3g03220
malus_domestica MD05G1334100.v1.1 MD10G1309000.v1.1
prunus_persica Prupe.4G031100_v2.0.a1 Prupe.4G031100_v2.0.a1
pyrus_communis pycom05g30580 pycom10g26040
rosa_chinensis RchiOBHm_Chr5g0004861
rosa_laevigata RLG00000031267
rosa_multiflora Rmu_sc0010871.1_g000001
rosa_roxburghii Rroxscaffold_1G00070760
rosa_rugosa Rorug04G0414800
rosa_samantha Rh5AG042300 Rh5BG042000 Rh5CG045800 Rh5DG042400
rosa_wichuraiana Rw5G004090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
Acc36I ACCTGC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 431
AccII CGCG 3 cut(s) 396, 411, 606
AclWI GGATC 1 cut(s) 814
AcoI YGGCCR 1 cut(s) 124
AcsI RAATTY 2 cut(s) 991, 1099
AcyI GRCGYC 2 cut(s) 120, 432
AdeI CACNNNGTG 1 cut(s) 1058
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 7 cut(s) 93, 481, 487, 610, 666, 787, 847
AgsI TTSAA 4 cut(s) 28, 857, 991, 1073
AjnI CCWGG 2 cut(s) 480, 959
AluBI AGCT 8 cut(s) 338, 358, 376, 418, 516, 633, 957, 1146
AluI AGCT 8 cut(s) 338, 358, 376, 418, 516, 633, 957, 1146
Alw21I GWGCWC 2 cut(s) 582, 959
Alw26I GTCTC 1 cut(s) 322
AlwI GGATC 1 cut(s) 814
Ama87I CYCGRG 2 cut(s) 347, 359
AoxI GGCC 4 cut(s) 96, 124, 657, 838
ApeKI GCWGC 4 cut(s) 198, 289, 401, 502
ApoI RAATTY 2 cut(s) 991, 1099
Asp700I GAANNNNTTC 1 cut(s) 1099
AspLEI GCGC 7 cut(s) 294, 396, 413, 434, 493, 608, 751
AspS9I GGNCC 3 cut(s) 96, 591, 838
AsuC2I CCSGG 3 cut(s) 17, 62, 773
AsuHPI GGTGA 1 cut(s) 269
AvaI CYCGRG 2 cut(s) 347, 359
AvaII GGWCC 1 cut(s) 591
BaeGI GKGCMC 1 cut(s) 35
BaeI ACNNNNGTAYC 2 cut(s) 233, 266
BanI GGYRCC 1 cut(s) 431
BanII GRGCYC 3 cut(s) 474, 694, 959
Bbv12I GWGCWC 2 cut(s) 582, 959
BbvI GCAGC 4 cut(s) 210, 301, 388, 489
BccI CCATC 4 cut(s) 110, 282, 767, 895
BceAI ACGGC 5 cut(s) 419, 428, 593, 730, 762
BciT130I CCWGG 2 cut(s) 482, 961
BcnI CCSGG 3 cut(s) 17, 62, 773
BcoDI GTCTC 1 cut(s) 322
BfaI CTAG 1 cut(s) 630
BfoI RGCGCY 4 cut(s) 295, 435, 494, 752
BfuAI ACCTGC 1 cut(s) 143
BglI GCCNNNNNGGC 1 cut(s) 614
BisI GCNGC 8 cut(s) 199, 290, 402, 503, 604, 615, 741, 785
BlsI GCNGC 8 cut(s) 200, 291, 403, 504, 605, 616, 742, 786
Bme1390I CCNGG 5 cut(s) 17, 62, 482, 773, 961
Bme18I GGWCC 1 cut(s) 591
BmeT110I CYCGRG 2 cut(s) 347, 359
BmgT120I GGNCC 3 cut(s) 96, 591, 838
BmiI GGNNCC 4 cut(s) 433, 592, 593, 1133
BmrFI CCNGG 5 cut(s) 17, 62, 482, 773, 961
BmsI GCATC 1 cut(s) 1008
BpmI CTGGAG 2 cut(s) 464, 943
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 3 cut(s) 17, 62, 773
BsaHI GRCGYC 2 cut(s) 120, 432
BsaJI CCNNGG 4 cut(s) 549, 583, 609, 960
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 7 cut(s) 93, 481, 487, 610, 666, 787, 847
Bse118I RCCGGY 2 cut(s) 122, 780
Bse1I ACTGG 1 cut(s) 247
BseBI CCWGG 2 cut(s) 482, 961
BseDI CCNNGG 4 cut(s) 549, 583, 609, 960
BseGI GGATG 4 cut(s) 102, 177, 923, 1023
BseLI CCNNNNNNNGG 7 cut(s) 93, 481, 487, 610, 666, 787, 847
BseMII CTCAG 3 cut(s) 23, 721, 1138
BseNI ACTGG 1 cut(s) 247
BseSI GKGCMC 1 cut(s) 35
BseXI GCAGC 4 cut(s) 210, 301, 388, 489
BseYI CCCAGC 2 cut(s) 300, 334
Bsh1236I CGCG 3 cut(s) 396, 411, 606
Bsh1285I CGRYCG 1 cut(s) 21
BshFI GGCC 4 cut(s) 98, 126, 659, 840
BshNI GGYRCC 1 cut(s) 431
BsiEI CGRYCG 1 cut(s) 21
BsiHKAI GWGCWC 2 cut(s) 582, 959
BsiHKCI CYCGRG 2 cut(s) 347, 359
BsiSI CCGG 6 cut(s) 17, 62, 123, 429, 773, 781
BslFI GGGAC 2 cut(s) 577, 725
BslI CCNNNNNNNGG 7 cut(s) 93, 481, 487, 610, 666, 787, 847
BsmAI GTCTC 1 cut(s) 322
BsmFI GGGAC 2 cut(s) 577, 725
BsnI GGCC 4 cut(s) 98, 126, 659, 840
BsoBI CYCGRG 2 cut(s) 347, 359
Bsp1286I GDGCHC 5 cut(s) 35, 474, 582, 694, 959
Bsp143I GATC 2 cut(s) 91, 819
BspANI GGCC 4 cut(s) 98, 126, 659, 840
BspCNI CTCAG 3 cut(s) 22, 720, 1137
BspFNI CGCG 3 cut(s) 396, 411, 606
BspLI GGNNCC 4 cut(s) 433, 592, 593, 1133
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 1 cut(s) 814
BspQI GCTCTTC 1 cut(s) 462
BspT107I GGYRCC 1 cut(s) 431
BsrFI RCCGGY 2 cut(s) 122, 780
BsrI ACTGG 1 cut(s) 247
BssAI RCCGGY 2 cut(s) 122, 780
BssECI CCNNGG 4 cut(s) 549, 583, 609, 960
BssMI GATC 2 cut(s) 91, 819
BssNI GRCGYC 2 cut(s) 120, 432
BssT1I CCWWGG 1 cut(s) 583
Bst2UI CCWGG 2 cut(s) 482, 961
Bst6I CTCTTC 3 cut(s) 79, 462, 891
BstACI GRCGYC 2 cut(s) 120, 432
BstC8I GCNNGC 4 cut(s) 113, 124, 287, 789
BstDEI CTNAG 3 cut(s) 9, 707, 1124
BstDSI CCRYGG 1 cut(s) 609
BstF5I GGATG 4 cut(s) 102, 177, 923, 1023
BstFNI CGCG 3 cut(s) 396, 411, 606
BstH2I RGCGCY 4 cut(s) 295, 435, 494, 752
BstHHI GCGC 7 cut(s) 294, 396, 413, 434, 493, 608, 751
BstKTI GATC 2 cut(s) 94, 822
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 2 cut(s) 91, 819
BstMCI CGRYCG 1 cut(s) 21
BstNI CCWGG 2 cut(s) 482, 961
BstSCI CCNGG 5 cut(s) 15, 60, 480, 771, 959
BstSLI GKGCMC 1 cut(s) 35
BstUI CGCG 3 cut(s) 396, 411, 606
BstV1I GCAGC 4 cut(s) 210, 301, 388, 489
BsuRI GGCC 4 cut(s) 98, 126, 659, 840
BtgI CCRYGG 1 cut(s) 609
BtsCI GGATG 4 cut(s) 102, 177, 923, 1023
BtsIMutI CAGTG 1 cut(s) 1132
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 4 cut(s) 113, 124, 287, 789
CfoI GCGC 7 cut(s) 294, 396, 413, 434, 493, 608, 751
Cfr10I RCCGGY 2 cut(s) 122, 780
Cfr13I GGNCC 3 cut(s) 96, 591, 838
CseI GACGC 3 cut(s) 128, 331, 407
Csp6I GTAC 1 cut(s) 250
CviAII CATG 1 cut(s) 1141
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 3 cut(s) 9, 707, 1124
DinI GGCGCC 1 cut(s) 433
DpnI GATC 2 cut(s) 93, 821
DpnII GATC 2 cut(s) 91, 819
DraIII CACNNNGTG 1 cut(s) 1058
EaeI YGGCCR 1 cut(s) 124
Eam1104I CTCTTC 3 cut(s) 79, 462, 891
EarI CTCTTC 3 cut(s) 79, 462, 891
EciI GGCGGA 1 cut(s) 430
Ecl136II GAGCTC 1 cut(s) 957
Eco130I CCWWGG 1 cut(s) 583
Eco24I GRGCYC 3 cut(s) 474, 694, 959
Eco47I GGWCC 1 cut(s) 591
Eco53kI GAGCTC 1 cut(s) 957
Eco88I CYCGRG 2 cut(s) 347, 359
EcoICRI GAGCTC 1 cut(s) 957
EcoO109I RGGNCCY 1 cut(s) 591
EcoRI GAATTC 1 cut(s) 991
EcoRII CCWGG 2 cut(s) 480, 959
EcoT14I CCWWGG 1 cut(s) 583
EcoT38I GRGCYC 3 cut(s) 474, 694, 959
EgeI GGCGCC 1 cut(s) 433
EheI GGCGCC 1 cut(s) 433
ErhI CCWWGG 1 cut(s) 583
FaeI CATG 1 cut(s) 1144
FaiI YATR 6 cut(s) 53, 68, 216, 673, 975, 1142
FalI AAGNNNNNCTT 2 cut(s) 525, 557
FaqI GGGAC 2 cut(s) 577, 725
FatI CATG 1 cut(s) 1140
FauI CCCGC 2 cut(s) 482, 780
Fnu4HI GCNGC 8 cut(s) 199, 290, 402, 503, 604, 615, 741, 785
FokI GGATG 4 cut(s) 89, 164, 930, 1030
FriOI GRGCYC 3 cut(s) 474, 694, 959
Fsp4HI GCNGC 8 cut(s) 199, 290, 402, 503, 604, 615, 741, 785
FspBI CTAG 1 cut(s) 630
GlaI GCGC 7 cut(s) 293, 395, 412, 433, 492, 607, 750
GluI GCNGC 8 cut(s) 199, 290, 402, 503, 604, 615, 741, 785
GsaI CCCAGC 2 cut(s) 304, 338
GsuI CTGGAG 2 cut(s) 464, 943
HaeII RGCGCY 4 cut(s) 295, 435, 494, 752
HaeIII GGCC 4 cut(s) 98, 126, 659, 840
HapII CCGG 6 cut(s) 17, 62, 123, 429, 773, 781
HgaI GACGC 3 cut(s) 128, 331, 407
HhaI GCGC 7 cut(s) 294, 396, 413, 434, 493, 608, 751
Hin1I GRCGYC 2 cut(s) 120, 432
Hin1II CATG 1 cut(s) 1144
Hin6I GCGC 7 cut(s) 292, 394, 411, 432, 491, 606, 749
HinP1I GCGC 7 cut(s) 292, 394, 411, 432, 491, 606, 749
HincII GTYRAC 3 cut(s) 460, 627, 652
HindII GTYRAC 3 cut(s) 460, 627, 652
HinfI GANTC 8 cut(s) 7, 350, 362, 425, 461, 567, 721, 833
HpaII CCGG 6 cut(s) 17, 62, 123, 429, 773, 781
HphI GGTGA 1 cut(s) 269
Hpy166II GTNNAC 3 cut(s) 460, 627, 652
Hpy188I TCNGA 3 cut(s) 58, 466, 824
Hpy188III TCNNGA 2 cut(s) 319, 725
Hpy8I GTNNAC 3 cut(s) 460, 627, 652
Hpy99I CGWCG 6 cut(s) 122, 325, 401, 527, 622, 748
HpyAV CCTTC 6 cut(s) 180, 230, 415, 727, 881, 1079
HpyCH4V TGCA 2 cut(s) 683, 882
HpyF3I CTNAG 3 cut(s) 9, 707, 1124
Hsp92I GRCGYC 2 cut(s) 120, 432
Hsp92II CATG 1 cut(s) 1144
HspAI GCGC 7 cut(s) 292, 394, 411, 432, 491, 606, 749
KasI GGCGCC 1 cut(s) 431
KflI GGGWCCC 1 cut(s) 591
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Kzo9I GATC 2 cut(s) 91, 819
LguI GCTCTTC 1 cut(s) 462
LmnI GCTCC 4 cut(s) 554, 585, 954, 962
Lsp1109I GCAGC 4 cut(s) 210, 301, 388, 489
LweI GCATC 1 cut(s) 1008
MaeI CTAG 1 cut(s) 630
MaeIII GTNAC 1 cut(s) 80
MalI GATC 2 cut(s) 93, 821
MboI GATC 2 cut(s) 91, 819
MboII GAAGA 5 cut(s) 66, 479, 908, 1085, 1095
MhlI GDGCHC 5 cut(s) 35, 474, 582, 694, 959
MluCI AATT 8 cut(s) 48, 141, 236, 684, 825, 991, 1099, 1108
Mly113I GGCGCC 1 cut(s) 432
MlyI GAGTC 5 cut(s) 359, 371, 434, 455, 730
MmeI TCCRAC 2 cut(s) 81, 130
MroNI GCCGGC 1 cut(s) 122
MroXI GAANNNNTTC 1 cut(s) 1099
MseI TTAA 1 cut(s) 912
MslI CAYNNNNRTG 1 cut(s) 676
MspI CCGG 6 cut(s) 17, 62, 123, 429, 773, 781
MspR9I CCNGG 5 cut(s) 17, 62, 482, 773, 961
MvaI CCWGG 2 cut(s) 482, 961
MvnI CGCG 3 cut(s) 396, 411, 606
NaeI GCCGGC 1 cut(s) 124
NarI GGCGCC 1 cut(s) 432
NciI CCSGG 3 cut(s) 17, 62, 773
NdeII GATC 2 cut(s) 91, 819
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 1 cut(s) 1144
NlaIV GGNNCC 4 cut(s) 433, 592, 593, 1133
NmeAIII GCCGAG 1 cut(s) 574
NmuCI GTSAC 1 cut(s) 80
PaeR7I CTCGAG 2 cut(s) 347, 359
PaqCI CACCTGC 1 cut(s) 143
PciSI GCTCTTC 1 cut(s) 462
PcsI WCGNNNNNNNCGW 2 cut(s) 366, 567
PdiI GCCGGC 1 cut(s) 124
PdmI GAANNNNTTC 1 cut(s) 1099
PfeI GAWTC 2 cut(s) 567, 833
PflFI GACNNNGTC 1 cut(s) 424
PfoI TCCNGGA 2 cut(s) 480, 771
PkrI GCNGC 8 cut(s) 200, 291, 403, 504, 605, 616, 742, 786
PleI GAGTC 5 cut(s) 358, 370, 433, 455, 729
PluTI GGCGCC 1 cut(s) 435
PpsI GAGTC 5 cut(s) 358, 370, 433, 455, 729
PpuMI RGGWCCY 1 cut(s) 591
Psp124BI GAGCTC 1 cut(s) 959
Psp5II RGGWCCY 1 cut(s) 591
Psp6I CCWGG 2 cut(s) 480, 959
PspFI CCCAGC 2 cut(s) 300, 334
PspGI CCWGG 2 cut(s) 480, 959
PspN4I GGNNCC 4 cut(s) 433, 592, 593, 1133
PspPI GGNCC 3 cut(s) 96, 591, 838
PspPPI RGGWCCY 1 cut(s) 591
PspXI VCTCGAGB 2 cut(s) 347, 359
PsyI GACNNNGTC 1 cut(s) 424
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
RseI CAYNNNNRTG 1 cut(s) 676
SacI GAGCTC 1 cut(s) 959
SapI GCTCTTC 1 cut(s) 462
SaqAI TTAA 1 cut(s) 912
SatI GCNGC 8 cut(s) 199, 290, 402, 503, 604, 615, 741, 785
Sau3AI GATC 2 cut(s) 91, 819
Sau96I GGNCC 3 cut(s) 96, 591, 838
SchI GAGTC 5 cut(s) 359, 371, 434, 455, 730
ScrFI CCNGG 5 cut(s) 17, 62, 482, 773, 961
SduI GDGCHC 5 cut(s) 35, 474, 582, 694, 959
SfaNI GCATC 1 cut(s) 1008
SfoI GGCGCC 1 cut(s) 433
Sfr274I CTCGAG 2 cut(s) 347, 359
SinI GGWCC 1 cut(s) 591
SlaI CTCGAG 2 cut(s) 347, 359
SmiMI CAYNNNNRTG 1 cut(s) 676
SmlI CTYRAG 3 cut(s) 347, 359, 449
SmoI CTYRAG 3 cut(s) 347, 359, 449
Sse9I AATT 8 cut(s) 48, 141, 236, 684, 825, 991, 1099, 1108
SspDI GGCGCC 1 cut(s) 431
SspMI CTAG 1 cut(s) 630
SstI GAGCTC 1 cut(s) 959
StyD4I CCNGG 5 cut(s) 15, 60, 480, 771, 959
StyI CCWWGG 1 cut(s) 583
TaqII GACCGA 2 cut(s) 437, 854
TasI AATT 8 cut(s) 48, 141, 236, 684, 825, 991, 1099, 1108
TauI GCSGC 4 cut(s) 606, 617, 743, 787
TfiI GAWTC 2 cut(s) 567, 833
Tru1I TTAA 1 cut(s) 912
Tru9I TTAA 1 cut(s) 912
TscAI CASTG 1 cut(s) 1132
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 4 cut(s) 198, 289, 401, 502
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 2 cut(s) 1053, 1098
TspGWI ACGGA 1 cut(s) 1030
TspRI CASTG 1 cut(s) 1132
Tth111I GACNNNGTC 1 cut(s) 424
VpaK11BI GGWCC 1 cut(s) 591
XapI RAATTY 2 cut(s) 991, 1099
XhoI CTCGAG 2 cut(s) 347, 359
XmnI GAANNNNTTC 1 cut(s) 1099
XspI CTAG 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.