RLG00000031267

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012930
Physical Location & Seq
Reverse (-)
3514193 .. 3515581
1389 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031267

Sequence Viewer

Length: 1389 bp
ATGTCAAATGTCAATTCAAGAGCTGAAAAAACCCAAACCCCCAATCTCCGAGACGAAAGACGAAAGGGGCAAAACCGTAATCTCAACATTTCGGGCAATAACAGAGTTCTGAAAGAAAAGAGTGGATTTTTGGTGGAGAGAAAGTCTCTGTTTTGTCTCCTCTATATTTTCTCCTCCCTTCAATTCCTCCAAATTTCACTTCTTCTTCTTCTTCTTCTTCTTCACACTTCCCCCGAAACCCTCGTAAACCCCAAAATGGCGACTCAATTCCCGGACGATTTCAAGTGCCCGATTTCGCTGGAAATAATGTCCGACCCGGTTATACTCCCTTCAGGTCACACCTTCGACCGGCCCAACATCCAACGCTGGCTCGACGCCGGCCACCGTACGTGCCCACTTACCAAACTACCCCTCCCCGAACACCCCTCCCTCATCCCCAACCACGCCCTCCGCAGCTTGATTTCCAATTATACCCTTCCCTCCCCTAGACAATTACACAACACCCAGCTCCCCCACCAGAGCCAGAGCCAGCTCAACCAGCAACCCCAAACCCTAATCGCGACGCTGACTTCGGCGTCGTCGAGCCTGGACTCGAAGCTGGAGTCGCTCGACCAGCTCAACCGCCTGTCGAAGCGGGACGCGGCGTTTCGCGCCAAGCTGACCGAGTCCGGCGCCGTCGCCGCCGTGCTCAAGTGCGTGGACTCCGACGAGCCGAGCCTGCAGGAGAAGGCTCTGACTCTGCTGCTGAATGTGAGCTTGGACGACGACAACAAGGTGGGTCTGGTGGCCGAAGGAGCGATTGCTAGAGTCGTGGCGGTGCTGCAAGGCGGCTCGCCGAACAGCCGGGCCGTGGCGGCGACGATGTTGACCAGCTTGGCCGTCGTGGAGGTCAACAAGGCCACAATCGGGTCGTACCCGTATGCAATTCGGGCTCTTGTTTCGCTTCTCAGGGACGGCAAGGGCCGGGAGAAGAAAGAGGCGGCGACGGCGCTTTATGCTATCTGTTCGTTTCCGGACAATCGGCGGCGGGCGGTGGAGTGCGGTGCTGTTTCAATTTTGATCCGAATTGCGGATTCGGGTTTGGAGCGGGCCGTTGAGGTTTTGGGCCTGTTGGCGAAAAGCAAGGATGGGAGAGAAGAGATGCAGAGGTTTAGTGGGTGTGTGAAGATTTTAGTGAGGGTTTTAGAGAACGGGAGCTCCAGGGGAATTCAGTATGCTCTGCTTACATTGAGTTCGCTTTGCCATTTCAGTGAGAAAATGTGTGTGGAAGCAAGAAAAGAAGGCGTCTTAGCGATTTGCGTTCGTTTGGTTGAAGATGATAATGAAAAGATTAGAAAAAATGCTTCTTATTTGATTCAGGTTCTGGATGGGAACCATTCCATGATTTGA

Protein Analysis

463

Amino Acids

50.86

Weight (kDa)

9.09

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 91 - 157 3.2e-20 U-box domain
zf-RING_UBOX PF13445 96 - 132 2.6e-06 RING-type zinc-finger
ARM_ARMC5 PF24768 186 - 357 5.1e-07 ARMC5
ARM_PUB PF25598 194 - 458 2.4e-24 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012930)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21350
fragaria_vesca FvH4_3g03220
malus_domestica MD05G1334100.v1.1 MD10G1309000.v1.1
prunus_persica Prupe.4G031100_v2.0.a1 Prupe.4G031100_v2.0.a1
pyrus_communis pycom05g30580 pycom10g26040
rosa_chinensis RchiOBHm_Chr5g0004861
rosa_laevigata RLG00000031267
rosa_multiflora Rmu_sc0010871.1_g000001
rosa_roxburghii Rroxscaffold_1G00070760
rosa_rugosa Rorug04G0414800
rosa_samantha Rh5AG042300 Rh5BG042000 Rh5CG045800 Rh5DG042400
rosa_wichuraiana Rw5G004090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 574
AccB1I GGYRCC 1 cut(s) 671
AccBSI CCGCTC 1 cut(s) 1087
AccII CGCG 3 cut(s) 560, 641, 651
AccIII TCCGGA 1 cut(s) 1012
AclWI GGATC 1 cut(s) 1054
AcoI YGGCCR 3 cut(s) 379, 786, 876
AcsI RAATTY 2 cut(s) 192, 1206
AcuI CTGAAG 1 cut(s) 315
AcyI GRCGYC 4 cut(s) 375, 575, 672, 1284
AfaI GTAC 2 cut(s) 388, 914
AfiI CCNNNNNNNGG 5 cut(s) 256, 348, 850, 906, 1069
AgsI TTSAA 5 cut(s) 18, 182, 283, 1053, 1313
AjnI CCWGG 2 cut(s) 585, 1199
AjuI GAANNNNNNNTTGG 4 cut(s) 183, 215, 740, 772
AloI GAACNNNNNNTCC 2 cut(s) 1181, 1213
Alw21I GWGCWC 2 cut(s) 690, 1199
Alw26I GTCTC 3 cut(s) 45, 150, 161
AlwI GGATC 1 cut(s) 1054
AlwNI CAGNNNCTG 1 cut(s) 1363
Aor13HI TCCGGA 1 cut(s) 1012
AoxI GGCC 9 cut(s) 350, 379, 786, 846, 876, 897, 961, 1089, 1105
ApeKI GCWGC 3 cut(s) 453, 742, 820
ApoI RAATTY 2 cut(s) 192, 1206
ArsI GACNNNNNNTTYG 4 cut(s) 553, 585, 587, 619
AspLEI GCGC 3 cut(s) 653, 674, 991
AspS9I GGNCC 5 cut(s) 351, 846, 961, 1089, 1105
AsuC2I CCSGG 4 cut(s) 272, 317, 845, 965
BaeGI GKGCMC 2 cut(s) 290, 395
BanI GGYRCC 1 cut(s) 671
BanII GRGCYC 2 cut(s) 934, 1199
Bbv12I GWGCWC 2 cut(s) 690, 1199
BbvI GCAGC 3 cut(s) 465, 729, 807
BccI CCATC 2 cut(s) 1121, 1361
BceAI ACGGC 7 cut(s) 659, 668, 833, 863, 970, 1002, 1076
BciT130I CCWGG 2 cut(s) 587, 1201
BcnI CCSGG 4 cut(s) 272, 317, 845, 965
BcoDI GTCTC 3 cut(s) 45, 150, 161
BfaI CTAG 2 cut(s) 486, 804
BfmI CTRYAG 1 cut(s) 719
BfoI RGCGCY 2 cut(s) 675, 992
BglI GCCNNNNNGGC 1 cut(s) 854
BisI GCNGC 9 cut(s) 454, 642, 681, 743, 821, 829, 855, 981, 1025
BlsI GCNGC 9 cut(s) 455, 643, 682, 744, 822, 830, 856, 982, 1026
Bme1390I CCNGG 6 cut(s) 272, 317, 587, 845, 965, 1201
BmgT120I GGNCC 5 cut(s) 351, 846, 961, 1089, 1105
BmiI GGNNCC 2 cut(s) 673, 1373
BmrFI CCNGG 6 cut(s) 272, 317, 587, 845, 965, 1201
BmsI GCATC 1 cut(s) 1131
BplI GAGNNNNNCTC 2 cut(s) 130, 162
BpmI CTGGAG 2 cut(s) 620, 1183
BpuEI CTTGAG 1 cut(s) 674
BpuMI CCSGG 4 cut(s) 272, 317, 845, 965
BsaAI YACGTR 1 cut(s) 390
BsaHI GRCGYC 4 cut(s) 375, 575, 672, 1284
BsaJI CCNNGG 2 cut(s) 849, 1200
BsaWI WCCGGW 1 cut(s) 1012
BsaXI ACNNNNNCTCC 6 cut(s) 128, 158, 396, 426, 1181, 1211
Bsc4I CCNNNNNNNGG 5 cut(s) 256, 348, 850, 906, 1069
Bse118I RCCGGY 2 cut(s) 348, 377
BseAI TCCGGA 1 cut(s) 1012
BseBI CCWGG 2 cut(s) 587, 1201
BseDI CCNNGG 2 cut(s) 849, 1200
BseGI GGATG 4 cut(s) 357, 432, 1132, 1372
BseLI CCNNNNNNNGG 5 cut(s) 256, 348, 850, 906, 1069
BseMII CTCAG 1 cut(s) 961
BseRI GAGGAG 2 cut(s) 149, 163
BseSI GKGCMC 2 cut(s) 290, 395
BseXI GCAGC 3 cut(s) 465, 729, 807
BseYI CCCAGC 1 cut(s) 504
Bsh1236I CGCG 3 cut(s) 560, 641, 651
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 9 cut(s) 352, 381, 788, 848, 878, 899, 963, 1091, 1107
BshNI GGYRCC 1 cut(s) 671
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 2 cut(s) 690, 1199
BsiSI CCGG 8 cut(s) 272, 317, 349, 378, 669, 844, 964, 1013
BsiWI CGTACG 1 cut(s) 386
BslFI GGGAC 2 cut(s) 650, 965
BslI CCNNNNNNNGG 5 cut(s) 256, 348, 850, 906, 1069
BsmAI GTCTC 3 cut(s) 45, 150, 161
BsmBI CGTCTC 1 cut(s) 45
BsmFI GGGAC 2 cut(s) 650, 965
BsnI GGCC 9 cut(s) 352, 381, 788, 848, 878, 899, 963, 1091, 1107
Bsp1286I GDGCHC 5 cut(s) 290, 395, 690, 934, 1199
Bsp13I TCCGGA 1 cut(s) 1012
Bsp143I GATC 1 cut(s) 1059
Bsp68I TCGCGA 1 cut(s) 560
BspANI GGCC 9 cut(s) 352, 381, 788, 848, 878, 899, 963, 1091, 1107
BspCNI CTCAG 1 cut(s) 960
BspEI TCCGGA 1 cut(s) 1012
BspFNI CGCG 3 cut(s) 560, 641, 651
BspLI GGNNCC 2 cut(s) 673, 1373
BspMAI CTGCAG 1 cut(s) 723
BspPI GGATC 1 cut(s) 1054
BspT107I GGYRCC 1 cut(s) 671
BsrBI CCGCTC 1 cut(s) 1087
BsrFI RCCGGY 2 cut(s) 348, 377
BssAI RCCGGY 2 cut(s) 348, 377
BssECI CCNNGG 2 cut(s) 849, 1200
BssMI GATC 1 cut(s) 1059
BssNI GRCGYC 4 cut(s) 375, 575, 672, 1284
Bst2UI CCWGG 2 cut(s) 587, 1201
Bst4CI ACNGT 2 cut(s) 77, 386
Bst6I CTCTTC 1 cut(s) 1131
BstACI GRCGYC 4 cut(s) 375, 575, 672, 1284
BstBAI YACGTR 1 cut(s) 390
BstC8I GCNNGC 7 cut(s) 368, 379, 530, 719, 833, 1029, 1089
BstDEI CTNAG 2 cut(s) 947, 1288
BstDSI CCRYGG 1 cut(s) 849
BstF5I GGATG 4 cut(s) 357, 432, 1132, 1372
BstFNI CGCG 3 cut(s) 560, 641, 651
BstH2I RGCGCY 2 cut(s) 675, 992
BstHHI GCGC 3 cut(s) 653, 674, 991
BstKTI GATC 1 cut(s) 1062
BstMAI GTCTC 3 cut(s) 45, 150, 161
BstMBI GATC 1 cut(s) 1059
BstMCI CGRYCG 1 cut(s) 349
BstNI CCWGG 2 cut(s) 587, 1201
BstSCI CCNGG 6 cut(s) 270, 315, 585, 843, 963, 1199
BstSFI CTRYAG 1 cut(s) 719
BstSLI GKGCMC 2 cut(s) 290, 395
BstUI CGCG 3 cut(s) 560, 641, 651
BstV1I GCAGC 3 cut(s) 465, 729, 807
BsuRI GGCC 9 cut(s) 352, 381, 788, 848, 878, 899, 963, 1091, 1107
BtgI CCRYGG 1 cut(s) 849
BtsCI GGATG 4 cut(s) 357, 432, 1132, 1372
BtsIMutI CAGTG 1 cut(s) 1255
BtuMI TCGCGA 1 cut(s) 560
Cac8I GCNNGC 7 cut(s) 368, 379, 530, 719, 833, 1029, 1089
CaiI CAGNNNCTG 1 cut(s) 1363
CfoI GCGC 3 cut(s) 653, 674, 991
Cfr10I RCCGGY 2 cut(s) 348, 377
Cfr13I GGNCC 5 cut(s) 351, 846, 961, 1089, 1105
CseI GACGC 5 cut(s) 383, 564, 571, 647, 1273
Csp6I GTAC 2 cut(s) 387, 913
CviAII CATG 1 cut(s) 1381
CviQI GTAC 2 cut(s) 387, 913
DdeI CTNAG 2 cut(s) 947, 1288
DinI GGCGCC 1 cut(s) 673
DpnI GATC 1 cut(s) 1061
DpnII GATC 1 cut(s) 1059
DrdI GACNNNNNNGTC 1 cut(s) 574
DseDI GACNNNNNNGTC 1 cut(s) 574
EaeI YGGCCR 3 cut(s) 379, 786, 876
Eam1104I CTCTTC 1 cut(s) 1131
EarI CTCTTC 1 cut(s) 1131
Ecl136II GAGCTC 1 cut(s) 1197
Eco24I GRGCYC 2 cut(s) 934, 1199
Eco53kI GAGCTC 1 cut(s) 1197
Eco57I CTGAAG 1 cut(s) 315
EcoICRI GAGCTC 1 cut(s) 1197
EcoRI GAATTC 1 cut(s) 1206
EcoRII CCWGG 2 cut(s) 585, 1199
EcoT38I GRGCYC 2 cut(s) 934, 1199
EgeI GGCGCC 1 cut(s) 673
EheI GGCGCC 1 cut(s) 673
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 1 cut(s) 1384
FaiI YATR 7 cut(s) 165, 323, 471, 921, 996, 1215, 1382
FaqI GGGAC 2 cut(s) 650, 965
FatI CATG 1 cut(s) 1380
FauI CCCGC 3 cut(s) 627, 1020, 1080
Fnu4HI GCNGC 9 cut(s) 454, 642, 681, 743, 821, 829, 855, 981, 1025
FokI GGATG 4 cut(s) 344, 419, 1139, 1379
FriOI GRGCYC 2 cut(s) 934, 1199
Fsp4HI GCNGC 9 cut(s) 454, 642, 681, 743, 821, 829, 855, 981, 1025
FspBI CTAG 2 cut(s) 486, 804
GlaI GCGC 3 cut(s) 652, 673, 990
GluI GCNGC 9 cut(s) 454, 642, 681, 743, 821, 829, 855, 981, 1025
GsaI CCCAGC 1 cut(s) 508
GsuI CTGGAG 2 cut(s) 620, 1183
HaeII RGCGCY 2 cut(s) 675, 992
HaeIII GGCC 9 cut(s) 352, 381, 788, 848, 878, 899, 963, 1091, 1107
HapII CCGG 8 cut(s) 272, 317, 349, 378, 669, 844, 964, 1013
HgaI GACGC 5 cut(s) 383, 564, 571, 647, 1273
HhaI GCGC 3 cut(s) 653, 674, 991
Hin1I GRCGYC 4 cut(s) 375, 575, 672, 1284
Hin1II CATG 1 cut(s) 1384
Hin6I GCGC 3 cut(s) 651, 672, 989
HinP1I GCGC 3 cut(s) 651, 672, 989
HincII GTYRAC 2 cut(s) 867, 892
HindII GTYRAC 2 cut(s) 867, 892
HinfI GANTC 9 cut(s) 262, 590, 602, 665, 701, 736, 807, 1073, 1354
HpaII CCGG 8 cut(s) 272, 317, 349, 378, 669, 844, 964, 1013
Hpy166II GTNNAC 4 cut(s) 247, 700, 867, 892
Hpy188I TCNGA 6 cut(s) 50, 111, 313, 706, 735, 1064
Hpy188III TCNNGA 4 cut(s) 18, 559, 1013, 1364
Hpy8I GTNNAC 4 cut(s) 247, 700, 867, 892
HpyAV CCTTC 7 cut(s) 188, 339, 352, 485, 721, 785, 1274
HpyCH4III ACNGT 2 cut(s) 77, 386
HpyCH4IV ACGT 1 cut(s) 389
HpyCH4V TGCA 4 cut(s) 721, 823, 923, 1144
HpyF3I CTNAG 2 cut(s) 947, 1288
HpySE526I ACGT 1 cut(s) 389
Hsp92I GRCGYC 4 cut(s) 375, 575, 672, 1284
Hsp92II CATG 1 cut(s) 1384
HspAI GCGC 3 cut(s) 651, 672, 989
KasI GGCGCC 1 cut(s) 671
Kpn2I TCCGGA 1 cut(s) 1012
KroI GCCGGC 1 cut(s) 377
KroNI GCCGGC 1 cut(s) 379
Kzo9I GATC 1 cut(s) 1059
LmnI GCTCC 5 cut(s) 513, 794, 1084, 1194, 1202
Lsp1109I GCAGC 3 cut(s) 465, 729, 807
LweI GCATC 1 cut(s) 1131
MaeI CTAG 2 cut(s) 486, 804
MaeII ACGT 1 cut(s) 389
MaeIII GTNAC 1 cut(s) 335
MalI GATC 1 cut(s) 1061
MbiI CCGCTC 1 cut(s) 1087
MboI GATC 1 cut(s) 1059
MhlI GDGCHC 5 cut(s) 290, 395, 690, 934, 1199
Mly113I GGCGCC 1 cut(s) 672
MlyI GAGTC 7 cut(s) 256, 584, 611, 674, 695, 730, 816
MmeI TCCRAC 3 cut(s) 336, 385, 729
MroI TCCGGA 1 cut(s) 1012
MroNI GCCGGC 1 cut(s) 377
MslI CAYNNNNRTG 1 cut(s) 1248
MspI CCGG 8 cut(s) 272, 317, 349, 378, 669, 844, 964, 1013
MspR9I CCNGG 6 cut(s) 272, 317, 587, 845, 965, 1201
MvaI CCWGG 2 cut(s) 587, 1201
MvnI CGCG 3 cut(s) 560, 641, 651
NaeI GCCGGC 1 cut(s) 379
NarI GGCGCC 1 cut(s) 672
NciI CCSGG 4 cut(s) 272, 317, 845, 965
NdeII GATC 1 cut(s) 1059
NgoMIV GCCGGC 1 cut(s) 377
NlaIII CATG 1 cut(s) 1384
NlaIV GGNNCC 2 cut(s) 673, 1373
NmeAIII GCCGAG 1 cut(s) 738
NmuCI GTSAC 1 cut(s) 335
NruI TCGCGA 1 cut(s) 560
PcsI WCGNNNNNNNCGW 1 cut(s) 578
PdiI GCCGGC 1 cut(s) 379
PfeI GAWTC 2 cut(s) 1073, 1354
Pfl23II CGTACG 1 cut(s) 386
PflFI GACNNNGTC 1 cut(s) 664
PfoI TCCNGGA 1 cut(s) 270
PkrI GCNGC 9 cut(s) 455, 643, 682, 744, 822, 830, 856, 982, 1026
PleI GAGTC 7 cut(s) 256, 584, 610, 673, 695, 730, 815
PluTI GGCGCC 1 cut(s) 675
PpsI GAGTC 7 cut(s) 256, 584, 610, 673, 695, 730, 815
Ppu21I YACGTR 1 cut(s) 390
Psp124BI GAGCTC 1 cut(s) 1199
Psp6I CCWGG 2 cut(s) 585, 1199
PspFI CCCAGC 1 cut(s) 504
PspGI CCWGG 2 cut(s) 585, 1199
PspLI CGTACG 1 cut(s) 386
PspN4I GGNNCC 2 cut(s) 673, 1373
PspPI GGNCC 5 cut(s) 351, 846, 961, 1089, 1105
PstI CTGCAG 1 cut(s) 723
PstNI CAGNNNCTG 1 cut(s) 1363
PsyI GACNNNGTC 1 cut(s) 664
RruI TCGCGA 1 cut(s) 560
RsaI GTAC 2 cut(s) 388, 914
RsaNI GTAC 2 cut(s) 387, 913
RseI CAYNNNNRTG 1 cut(s) 1248
SacI GAGCTC 1 cut(s) 1199
SatI GCNGC 9 cut(s) 454, 642, 681, 743, 821, 829, 855, 981, 1025
Sau3AI GATC 1 cut(s) 1059
Sau96I GGNCC 5 cut(s) 351, 846, 961, 1089, 1105
SbfI CCTGCAGG 1 cut(s) 723
SchI GAGTC 7 cut(s) 256, 584, 611, 674, 695, 730, 816
ScrFI CCNGG 6 cut(s) 272, 317, 587, 845, 965, 1201
SdaI CCTGCAGG 1 cut(s) 723
SduI GDGCHC 5 cut(s) 290, 395, 690, 934, 1199
SfaNI GCATC 1 cut(s) 1131
SfcI CTRYAG 1 cut(s) 719
SfoI GGCGCC 1 cut(s) 673
SmiMI CAYNNNNRTG 1 cut(s) 1248
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
Sse8387I CCTGCAGG 1 cut(s) 723
SspDI GGCGCC 1 cut(s) 671
SspMI CTAG 2 cut(s) 486, 804
SstI GAGCTC 1 cut(s) 1199
StyD4I CCNGG 6 cut(s) 270, 315, 585, 843, 963, 1199
TaaI ACNGT 2 cut(s) 77, 386
TaiI ACGT 1 cut(s) 392
TaqI TCGA 6 cut(s) 345, 372, 581, 593, 609, 629
TaqII GACCGA 1 cut(s) 677
TauI GCSGC 6 cut(s) 644, 683, 831, 857, 983, 1027
TfiI GAWTC 2 cut(s) 1073, 1354
TscAI CASTG 1 cut(s) 1255
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 3 cut(s) 453, 742, 820
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 1 cut(s) 1338
TspRI CASTG 1 cut(s) 1255
Tth111I GACNNNGTC 1 cut(s) 664
XapI RAATTY 2 cut(s) 192, 1206
XspI CTAG 2 cut(s) 486, 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.