Rroxscaffold_1G00070760

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012930
Physical Location & Seq
Forward (+)
91693384 .. 91695591
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00070760.1

Sequence Viewer

Length: 972 bp
ATGGCGACTCAATTCCCGGACGATTTCAAGTGCCCGATTTCGCTGGAAATAATGTCGGACCCGGTTATACTCCCTTTCAGGTCACACCTTCGACCGGCCCAACATCCAACGCTGGCTCGACGCCGGCCACCGTACTTGCCCGCTTACCAAACTACCCCTCCCCGAACACCCGTCCCTCATCCCCAACCACGCCCTCCGCAGCTTGATTTCCAATTATACCCCTTCCCCTCCCCTACACAATTACACAACACCCAGCTCCCCCACCAGAGCCAGGCCAACCAGCAACCCCAAACCCTAATCGCGACCCTGACTTCGGCGGCGTCGAGCCTGGACTCGAAGCTCGAGTCGCTCGACCAGCTCAACCGCCTGTCGAAGCGGGACGCTGCGTTTCGCGCCAAGCTGACGGAGTCCGGCGCCGTCGCCGCCGTGCTGAAGTGCGTGGACTCCGACGAGCCGAGCCTGCAGGAGAAGGCTCTGACTCTGCTGCTGAATGTGAGCTTGGACGACGACAACAAGGTGGGTCTGGTGGCGGAAGGAGCGATTGCTAGAGTCGTGGCGGTGCTGCAAGGCGGCTCGCCGAACAGCCGGGCGGTCGCGGCGACGATGTTGACCAGCCTGGCCGTCGTGGAGGTGAACAAGGCCACAATCGGGTCGTACCCGTATGCAATTCGGGCTCTTGTTTCGCTTCTCAGGGACGGCAAGGGGCGGGAGAAGAAAGAGGCGGCGACGGCGCTTTATGCTATCTGTTCGTTTCCGGACAATCGGCGGCGGGCGGTGGAGTGCGGTGCGGTTTCGATTTTGATCCGAATTGCGGATTCGGGTTTGGAGCGGGCCGTTGAGGTTTTGGGCCTGTTGGCGAAAAGCAAGGATGGGAGAGAAGAGATGCAGAGGTTTAGTGGGTGTGTGAGGATTTTAGTGAGGGTTTTAGAGAATGGGAGCTCCAGGGGGTTCAGTATGCTCTGCTTACATTGA

Protein Analysis

323

Amino Acids

35.18

Weight (kDa)

9.16

Isoelectric Point (pI)

54.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 110 - 309 3.1e-20 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012930)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21350
fragaria_vesca FvH4_3g03220
malus_domestica MD05G1334100.v1.1 MD10G1309000.v1.1
prunus_persica Prupe.4G031100_v2.0.a1 Prupe.4G031100_v2.0.a1
pyrus_communis pycom05g30580 pycom10g26040
rosa_chinensis RchiOBHm_Chr5g0004861
rosa_laevigata RLG00000031267
rosa_multiflora Rmu_sc0010871.1_g000001
rosa_roxburghii Rroxscaffold_1G00070760
rosa_rugosa Rorug04G0414800
rosa_samantha Rh5AG042300 Rh5BG042000 Rh5CG045800 Rh5DG042400
rosa_wichuraiana Rw5G004090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 413
AccBSI CCGCTC 1 cut(s) 829
AccII CGCG 3 cut(s) 302, 393, 596
AccIII TCCGGA 1 cut(s) 754
AclWI GGATC 1 cut(s) 796
AcoI YGGCCR 2 cut(s) 125, 618
AcuI CTGAAG 1 cut(s) 452
AcyI GRCGYC 3 cut(s) 121, 320, 414
AfaI GTAC 2 cut(s) 134, 656
AfiI CCNNNNNNNGG 5 cut(s) 94, 271, 313, 648, 811
AgsI TTSAA 1 cut(s) 28
AjnI CCWGG 4 cut(s) 270, 327, 615, 941
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
AluBI AGCT 7 cut(s) 202, 256, 340, 358, 400, 498, 939
AluI AGCT 7 cut(s) 202, 256, 340, 358, 400, 498, 939
Alw21I GWGCWC 1 cut(s) 941
AlwI GGATC 1 cut(s) 796
Ama87I CYCGRG 1 cut(s) 341
Aor13HI TCCGGA 1 cut(s) 754
AoxI GGCC 7 cut(s) 96, 125, 273, 618, 639, 831, 847
ApeKI GCWGC 4 cut(s) 199, 383, 484, 562
ArsI GACNNNNNNTTYG 4 cut(s) 295, 327, 329, 361
AspLEI GCGC 3 cut(s) 395, 416, 733
AspS9I GGNCC 4 cut(s) 58, 97, 831, 847
AsuC2I CCSGG 3 cut(s) 17, 62, 587
AsuHPI GGTGA 1 cut(s) 643
AvaI CYCGRG 1 cut(s) 341
AvaII GGWCC 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 35
BanI GGYRCC 1 cut(s) 413
BanII GRGCYC 2 cut(s) 676, 941
Bbv12I GWGCWC 1 cut(s) 941
BbvI GCAGC 4 cut(s) 211, 370, 471, 549
BccI CCATC 1 cut(s) 863
BceAI ACGGC 6 cut(s) 401, 410, 605, 712, 744, 818
BciT130I CCWGG 4 cut(s) 272, 329, 617, 943
BcnI CCSGG 3 cut(s) 17, 62, 587
BfaI CTAG 1 cut(s) 546
BfmI CTRYAG 1 cut(s) 461
BfoI RGCGCY 2 cut(s) 417, 734
Bme1390I CCNGG 7 cut(s) 17, 62, 272, 329, 587, 617, 943
Bme18I GGWCC 1 cut(s) 58
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 4 cut(s) 58, 97, 831, 847
BmiI GGNNCC 2 cut(s) 60, 415
BmrFI CCNGG 7 cut(s) 17, 62, 272, 329, 587, 617, 943
BmsI GCATC 1 cut(s) 873
BpmI CTGGAG 1 cut(s) 925
BpuMI CCSGG 3 cut(s) 17, 62, 587
BsaBI GATNNNNATC 1 cut(s) 800
BsaHI GRCGYC 3 cut(s) 121, 320, 414
BsaJI CCNNGG 1 cut(s) 942
BsaWI WCCGGW 1 cut(s) 754
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 5 cut(s) 94, 271, 313, 648, 811
Bse118I RCCGGY 2 cut(s) 94, 123
Bse8I GATNNNNATC 1 cut(s) 800
BseAI TCCGGA 1 cut(s) 754
BseBI CCWGG 4 cut(s) 272, 329, 617, 943
BseDI CCNNGG 1 cut(s) 942
BseGI GGATG 3 cut(s) 103, 178, 874
BseJI GATNNNNATC 1 cut(s) 800
BseLI CCNNNNNNNGG 5 cut(s) 94, 271, 313, 648, 811
BseMII CTCAG 1 cut(s) 703
BseSI GKGCMC 1 cut(s) 35
BseXI GCAGC 4 cut(s) 211, 370, 471, 549
BseYI CCCAGC 1 cut(s) 252
Bsh1236I CGCG 3 cut(s) 302, 393, 596
Bsh1285I CGRYCG 2 cut(s) 95, 594
BshFI GGCC 7 cut(s) 98, 127, 275, 620, 641, 833, 849
BshNI GGYRCC 1 cut(s) 413
BsiEI CGRYCG 2 cut(s) 95, 594
BsiHKAI GWGCWC 1 cut(s) 941
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 7 cut(s) 17, 62, 95, 124, 411, 586, 755
BslFI GGGAC 3 cut(s) 158, 392, 707
BslI CCNNNNNNNGG 5 cut(s) 94, 271, 313, 648, 811
BsmFI GGGAC 3 cut(s) 158, 392, 707
BsnI GGCC 7 cut(s) 98, 127, 275, 620, 641, 833, 849
BsoBI CYCGRG 1 cut(s) 341
Bsp1286I GDGCHC 3 cut(s) 35, 676, 941
Bsp13I TCCGGA 1 cut(s) 754
Bsp143I GATC 1 cut(s) 801
Bsp68I TCGCGA 1 cut(s) 302
BspANI GGCC 7 cut(s) 98, 127, 275, 620, 641, 833, 849
BspCNI CTCAG 1 cut(s) 702
BspEI TCCGGA 1 cut(s) 754
BspFNI CGCG 3 cut(s) 302, 393, 596
BspLI GGNNCC 2 cut(s) 60, 415
BspMAI CTGCAG 1 cut(s) 465
BspPI GGATC 1 cut(s) 796
BspT107I GGYRCC 1 cut(s) 413
BsrBI CCGCTC 1 cut(s) 829
BsrFI RCCGGY 2 cut(s) 94, 123
BssAI RCCGGY 2 cut(s) 94, 123
BssECI CCNNGG 1 cut(s) 942
BssMI GATC 1 cut(s) 801
BssNI GRCGYC 3 cut(s) 121, 320, 414
Bst2UI CCWGG 4 cut(s) 272, 329, 617, 943
Bst4CI ACNGT 1 cut(s) 132
Bst6I CTCTTC 1 cut(s) 873
BstACI GRCGYC 3 cut(s) 121, 320, 414
BstC8I GCNNGC 7 cut(s) 114, 125, 141, 461, 575, 771, 831
BstDEI CTNAG 1 cut(s) 689
BstF5I GGATG 3 cut(s) 103, 178, 874
BstFNI CGCG 3 cut(s) 302, 393, 596
BstH2I RGCGCY 2 cut(s) 417, 734
BstHHI GCGC 3 cut(s) 395, 416, 733
BstKTI GATC 1 cut(s) 804
BstMBI GATC 1 cut(s) 801
BstMCI CGRYCG 2 cut(s) 95, 594
BstNI CCWGG 4 cut(s) 272, 329, 617, 943
BstSCI CCNGG 7 cut(s) 15, 60, 270, 327, 585, 615, 941
BstSFI CTRYAG 1 cut(s) 461
BstSLI GKGCMC 1 cut(s) 35
BstUI CGCG 3 cut(s) 302, 393, 596
BstV1I GCAGC 4 cut(s) 211, 370, 471, 549
BsuRI GGCC 7 cut(s) 98, 127, 275, 620, 641, 833, 849
BtsCI GGATG 3 cut(s) 103, 178, 874
BtuMI TCGCGA 1 cut(s) 302
Cac8I GCNNGC 7 cut(s) 114, 125, 141, 461, 575, 771, 831
CfoI GCGC 3 cut(s) 395, 416, 733
Cfr10I RCCGGY 2 cut(s) 94, 123
Cfr13I GGNCC 4 cut(s) 58, 97, 831, 847
CseI GACGC 3 cut(s) 129, 309, 389
Csp6I GTAC 2 cut(s) 133, 655
CspCI CAANNNNNGTGG 2 cut(s) 117, 152
CviQI GTAC 2 cut(s) 133, 655
DdeI CTNAG 1 cut(s) 689
DinI GGCGCC 1 cut(s) 415
DpnI GATC 1 cut(s) 803
DpnII GATC 1 cut(s) 801
EaeI YGGCCR 2 cut(s) 125, 618
Eam1104I CTCTTC 1 cut(s) 873
EarI CTCTTC 1 cut(s) 873
EciI GGCGGA 1 cut(s) 545
Ecl136II GAGCTC 1 cut(s) 939
Eco24I GRGCYC 2 cut(s) 676, 941
Eco47I GGWCC 1 cut(s) 58
Eco53kI GAGCTC 1 cut(s) 939
Eco57I CTGAAG 1 cut(s) 452
Eco88I CYCGRG 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 939
EcoRII CCWGG 4 cut(s) 270, 327, 615, 941
EcoT38I GRGCYC 2 cut(s) 676, 941
EgeI GGCGCC 1 cut(s) 415
EheI GGCGCC 1 cut(s) 415
FaiI YATR 5 cut(s) 68, 217, 663, 738, 956
FaqI GGGAC 3 cut(s) 158, 392, 707
FauI CCCGC 5 cut(s) 148, 369, 699, 762, 822
FokI GGATG 3 cut(s) 90, 165, 881
FriOI GRGCYC 2 cut(s) 676, 941
FspBI CTAG 1 cut(s) 546
GlaI GCGC 3 cut(s) 394, 415, 732
GsaI CCCAGC 1 cut(s) 256
GsuI CTGGAG 1 cut(s) 925
HaeII RGCGCY 2 cut(s) 417, 734
HaeIII GGCC 7 cut(s) 98, 127, 275, 620, 641, 833, 849
HapII CCGG 7 cut(s) 17, 62, 95, 124, 411, 586, 755
HgaI GACGC 3 cut(s) 129, 309, 389
HhaI GCGC 3 cut(s) 395, 416, 733
Hin1I GRCGYC 3 cut(s) 121, 320, 414
Hin6I GCGC 3 cut(s) 393, 414, 731
HinP1I GCGC 3 cut(s) 393, 414, 731
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HinfI GANTC 8 cut(s) 7, 332, 344, 407, 443, 478, 549, 815
HpaII CCGG 7 cut(s) 17, 62, 95, 124, 411, 586, 755
HphI GGTGA 1 cut(s) 643
Hpy166II GTNNAC 3 cut(s) 442, 609, 634
Hpy188I TCNGA 4 cut(s) 58, 448, 477, 806
Hpy188III TCNNGA 2 cut(s) 301, 755
Hpy8I GTNNAC 3 cut(s) 442, 609, 634
Hpy99I CGWCG 8 cut(s) 123, 325, 422, 452, 509, 604, 626, 730
HpyAV CCTTC 4 cut(s) 98, 232, 463, 527
HpyCH4III ACNGT 1 cut(s) 132
HpyCH4V TGCA 4 cut(s) 463, 565, 665, 886
HpyF3I CTNAG 1 cut(s) 689
Hsp92I GRCGYC 3 cut(s) 121, 320, 414
HspAI GCGC 3 cut(s) 393, 414, 731
KasI GGCGCC 1 cut(s) 413
Kpn2I TCCGGA 1 cut(s) 754
KroI GCCGGC 1 cut(s) 123
KroNI GCCGGC 1 cut(s) 125
Kzo9I GATC 1 cut(s) 801
LmnI GCTCC 5 cut(s) 261, 536, 826, 936, 944
Lsp1109I GCAGC 4 cut(s) 211, 370, 471, 549
LweI GCATC 1 cut(s) 873
MaeI CTAG 1 cut(s) 546
MaeIII GTNAC 1 cut(s) 81
MalI GATC 1 cut(s) 803
MbiI CCGCTC 1 cut(s) 829
MboI GATC 1 cut(s) 801
MboII GAAGA 2 cut(s) 724, 890
MhlI GDGCHC 3 cut(s) 35, 676, 941
MluCI AATT 5 cut(s) 11, 212, 239, 666, 807
Mly113I GGCGCC 1 cut(s) 414
MlyI GAGTC 6 cut(s) 326, 353, 416, 437, 472, 558
MmeI TCCRAC 3 cut(s) 36, 131, 471
MroI TCCGGA 1 cut(s) 754
MroNI GCCGGC 1 cut(s) 123
MspI CCGG 7 cut(s) 17, 62, 95, 124, 411, 586, 755
MspR9I CCNGG 7 cut(s) 17, 62, 272, 329, 587, 617, 943
MvaI CCWGG 4 cut(s) 272, 329, 617, 943
MvnI CGCG 3 cut(s) 302, 393, 596
NaeI GCCGGC 1 cut(s) 125
NarI GGCGCC 1 cut(s) 414
NciI CCSGG 3 cut(s) 17, 62, 587
NdeII GATC 1 cut(s) 801
NgoMIV GCCGGC 1 cut(s) 123
NlaIV GGNNCC 2 cut(s) 60, 415
NmeAIII GCCGAG 1 cut(s) 480
NmuCI GTSAC 1 cut(s) 81
NruI TCGCGA 1 cut(s) 302
PaeR7I CTCGAG 1 cut(s) 341
PcsI WCGNNNNNNNCGW 2 cut(s) 320, 348
PdiI GCCGGC 1 cut(s) 125
PfeI GAWTC 1 cut(s) 815
PflFI GACNNNGTC 1 cut(s) 406
PfoI TCCNGGA 1 cut(s) 15
PleI GAGTC 6 cut(s) 326, 352, 415, 437, 472, 557
PluTI GGCGCC 1 cut(s) 417
PpsI GAGTC 6 cut(s) 326, 352, 415, 437, 472, 557
Psp124BI GAGCTC 1 cut(s) 941
Psp6I CCWGG 4 cut(s) 270, 327, 615, 941
PspFI CCCAGC 1 cut(s) 252
PspGI CCWGG 4 cut(s) 270, 327, 615, 941
PspN4I GGNNCC 2 cut(s) 60, 415
PspPI GGNCC 4 cut(s) 58, 97, 831, 847
PspXI VCTCGAGB 1 cut(s) 341
PstI CTGCAG 1 cut(s) 465
PsyI GACNNNGTC 1 cut(s) 406
RruI TCGCGA 1 cut(s) 302
RsaI GTAC 2 cut(s) 134, 656
RsaNI GTAC 2 cut(s) 133, 655
SacI GAGCTC 1 cut(s) 941
Sau3AI GATC 1 cut(s) 801
Sau96I GGNCC 4 cut(s) 58, 97, 831, 847
SbfI CCTGCAGG 1 cut(s) 465
SchI GAGTC 6 cut(s) 326, 353, 416, 437, 472, 558
ScrFI CCNGG 7 cut(s) 17, 62, 272, 329, 587, 617, 943
SdaI CCTGCAGG 1 cut(s) 465
SduI GDGCHC 3 cut(s) 35, 676, 941
SfaNI GCATC 1 cut(s) 873
SfcI CTRYAG 1 cut(s) 461
SfoI GGCGCC 1 cut(s) 415
Sfr274I CTCGAG 1 cut(s) 341
SinI GGWCC 1 cut(s) 58
SlaI CTCGAG 1 cut(s) 341
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse8387I CCTGCAGG 1 cut(s) 465
Sse9I AATT 5 cut(s) 11, 212, 239, 666, 807
SspDI GGCGCC 1 cut(s) 413
SspMI CTAG 1 cut(s) 546
SstI GAGCTC 1 cut(s) 941
StyD4I CCNGG 7 cut(s) 15, 60, 270, 327, 585, 615, 941
TaaI ACNGT 1 cut(s) 132
TaqI TCGA 8 cut(s) 91, 118, 323, 335, 342, 351, 371, 794
TasI AATT 5 cut(s) 11, 212, 239, 666, 807
TauI GCSGC 6 cut(s) 320, 425, 573, 599, 725, 769
TfiI GAWTC 1 cut(s) 815
TseFI GTSAC 1 cut(s) 81
TseI GCWGC 4 cut(s) 199, 383, 484, 562
Tsp45I GTSAC 1 cut(s) 81
TspGWI ACGGA 1 cut(s) 419
Tth111I GACNNNGTC 1 cut(s) 406
VpaK11BI GGWCC 1 cut(s) 58
XhoI CTCGAG 1 cut(s) 341
XspI CTAG 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.