AT4G21450

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007622
Physical Location & Seq
Forward (+)
11425933 .. 11428435
2503 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G21450.2

Sequence Viewer

Length: 888 bp
ATGACTATGACGGAGGAGAAACCAACTTCCGACGGCAAAGGAGGTTGGGGTTTCTTCAAGATTCCATTTCGTAACTCAAGCGGTCACAGAAACGCCGCTTCAAGTGCTGCTACTTCTCCGTTTCCATCTGGAGCTTCTTCTTCTTCTACTTCTTCTCATCTACATAACCATCATCAACATCATCATCAACATCACCATCAGCACCATCATCAGCTTGGTTATAACGGTCCTCACGGTGATGGATCGGGTCAAAATCAACATCCGACTCCTTCTCCTTCAGTTTCATCTGTTGCTAAGTCTTTTCTTCCTACTAAACGAAGATTGAAGCTTGATCCTTCTGAGAAACTCTATTTTCCTTATGAGCCTGGGAAGCAAGTTAGGAGTGCTATTAAGATTAAAAATACAAGCAAATCACATGTAGCCTTCAAGTTTCAAACAACTGCGCCGAAGAGTTGCTTTATGCGTCCACCTGGTGCTATTCTTGCTCCTGGAGAGACTATTATTGCTACTGTGTTTAAATTTGTTGAGCCTCCTGAGAATAATGAGAAGCCAATGGATCAAAGGAGCAGAGTGAAGTTTAAAATCATGAGCCTGAAGGTGAAAGGTCCAATGGACTATGTGCCTGAGCTGTTCGATGAGCAAAAGGATGATGTCTCTAAGGAGCAAATATTGCGTGTGATTTTCTTGGATCCTGAGCGCTCCAACCCTGCATTGGAAAAACTGAAGCGTCAATTAGCAGAAGCGGATGCAGCGGTGGAGGCACGAAAGAAACCTCCAGAGGAAACAGGTCCAAAGATGATTGGAGAAGGGCTTGTGATCGATGAATGGAAGGAGCGTAGAGAGAGATATCTTGCACAGCAACAAGGCGAAGGAGCTGACTCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

295

Amino Acids

32.96

Weight (kDa)

9.39

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 108 - 207 6.5e-23 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 222
AciI CCGC 4 cut(s) 81, 96, 743, 752
AclWI GGATC 5 cut(s) 250, 326, 564, 683, 696
AcsI RAATTY 1 cut(s) 518
AcuI CTGAAG 3 cut(s) 261, 614, 743
AdeI CACNNNGTG 1 cut(s) 473
AfeI AGCGCT 1 cut(s) 698
AfiI CCNNNNNNNGG 1 cut(s) 712
AflIII ACRYGT 1 cut(s) 415
AgsI TTSAA 5 cut(s) 58, 102, 325, 427, 434
AjnI CCWGG 3 cut(s) 364, 469, 487
AluBI AGCT 5 cut(s) 134, 214, 328, 628, 875
AluI AGCT 5 cut(s) 134, 214, 328, 628, 875
Alw26I GTCTC 2 cut(s) 488, 658
AlwI GGATC 5 cut(s) 250, 326, 564, 683, 696
Aor51HI AGCGCT 1 cut(s) 698
ApeKI GCWGC 2 cut(s) 107, 749
ApoI RAATTY 1 cut(s) 518
AspLEI GCGC 2 cut(s) 445, 699
AspS9I GGNCC 3 cut(s) 227, 605, 788
AsuHPI GGTGA 3 cut(s) 185, 248, 610
AvaII GGWCC 3 cut(s) 227, 605, 788
BamHI GGATCC 1 cut(s) 688
BbvI GCAGC 2 cut(s) 94, 761
BccI CCATC 5 cut(s) 133, 177, 204, 213, 233
BceAI ACGGC 1 cut(s) 49
BciT130I CCWGG 3 cut(s) 366, 471, 489
BcoDI GTCTC 2 cut(s) 488, 658
BfoI RGCGCY 1 cut(s) 700
BisI GCNGC 3 cut(s) 96, 108, 750
BlsI GCNGC 3 cut(s) 97, 109, 751
Bme1390I CCNGG 3 cut(s) 366, 471, 489
Bme18I GGWCC 3 cut(s) 227, 605, 788
BmgT120I GGNCC 3 cut(s) 227, 605, 788
BmiI GGNNCC 1 cut(s) 690
BmrFI CCNGG 3 cut(s) 366, 471, 489
BmsI GCATC 1 cut(s) 736
BpmI CTGGAG 3 cut(s) 150, 510, 759
Bpu10I CCTNAGC 2 cut(s) 624, 693
BpuEI CTTGAG 1 cut(s) 61
Bsa29I ATCGAT 1 cut(s) 819
BsaJI CCNNGG 1 cut(s) 365
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bsc4I CCNNNNNNNGG 1 cut(s) 712
BseBI CCWGG 3 cut(s) 366, 471, 489
BseCI ATCGAT 1 cut(s) 819
BseDI CCNNGG 1 cut(s) 365
BseGI GGATG 3 cut(s) 259, 652, 751
BseLI CCNNNNNNNGG 1 cut(s) 712
BseMII CTCAG 4 cut(s) 330, 525, 615, 684
BseRI GAGGAG 1 cut(s) 29
BseXI GCAGC 2 cut(s) 94, 761
BshVI ATCGAT 1 cut(s) 819
BslI CCNNNNNNNGG 1 cut(s) 712
BsmAI GTCTC 2 cut(s) 488, 658
Bsp143I GATC 5 cut(s) 242, 331, 556, 688, 816
BspACI CCGC 4 cut(s) 81, 96, 743, 752
BspCNI CTCAG 4 cut(s) 331, 526, 616, 685
BspDI ATCGAT 1 cut(s) 819
BspHI TCATGA 1 cut(s) 585
BspLI GGNNCC 1 cut(s) 690
BspPI GGATC 5 cut(s) 250, 326, 564, 683, 696
BssECI CCNNGG 1 cut(s) 365
BssMI GATC 5 cut(s) 242, 331, 556, 688, 816
Bst2UI CCWGG 3 cut(s) 366, 471, 489
Bst4CI ACNGT 3 cut(s) 227, 236, 511
Bst6I CTCTTC 1 cut(s) 443
BstAPI GCANNNNNTGC 1 cut(s) 670
BstDEI CTNAG 6 cut(s) 294, 339, 534, 624, 657, 693
BstF5I GGATG 3 cut(s) 259, 652, 751
BstH2I RGCGCY 1 cut(s) 700
BstHHI GCGC 2 cut(s) 445, 699
BstKTI GATC 5 cut(s) 245, 334, 559, 691, 819
BstMAI GTCTC 2 cut(s) 488, 658
BstMBI GATC 5 cut(s) 242, 331, 556, 688, 816
BstMWI GCNNNNNNNGC 6 cut(s) 104, 370, 482, 670, 749, 758
BstNI CCWGG 3 cut(s) 366, 471, 489
BstNSI RCATGY 1 cut(s) 419
BstSCI CCNGG 3 cut(s) 364, 469, 487
BstV1I GCAGC 2 cut(s) 94, 761
BstX2I RGATCY 1 cut(s) 688
BstYI RGATCY 1 cut(s) 688
Bsu15I ATCGAT 1 cut(s) 819
BsuTUI ATCGAT 1 cut(s) 819
BtsCI GGATG 3 cut(s) 259, 652, 751
CciI TCATGA 1 cut(s) 585
CfoI GCGC 2 cut(s) 445, 699
Cfr13I GGNCC 3 cut(s) 227, 605, 788
ClaI ATCGAT 1 cut(s) 819
CseI GACGC 2 cut(s) 452, 716
CsiI ACCWGGT 1 cut(s) 469
CviAII CATG 2 cut(s) 416, 586
DdeI CTNAG 6 cut(s) 294, 339, 534, 624, 657, 693
DpnI GATC 5 cut(s) 244, 333, 558, 690, 818
DpnII GATC 5 cut(s) 242, 331, 556, 688, 816
DraI TTTAAA 2 cut(s) 517, 580
DraIII CACNNNGTG 1 cut(s) 473
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
Eco32I GATATC 1 cut(s) 848
Eco47I GGWCC 3 cut(s) 227, 605, 788
Eco47III AGCGCT 1 cut(s) 698
Eco57I CTGAAG 3 cut(s) 261, 614, 743
EcoRII CCWGG 3 cut(s) 364, 469, 487
EcoRV GATATC 1 cut(s) 848
FaeI CATG 2 cut(s) 419, 589
FaiI YATR 8 cut(s) 8, 165, 222, 360, 417, 461, 587, 618
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FatI CATG 2 cut(s) 415, 585
Fnu4HI GCNGC 3 cut(s) 96, 108, 750
FokI GGATG 3 cut(s) 246, 659, 758
Fsp4HI GCNGC 3 cut(s) 96, 108, 750
GlaI GCGC 2 cut(s) 444, 698
GluI GCNGC 3 cut(s) 96, 108, 750
GsuI CTGGAG 3 cut(s) 150, 510, 759
HaeII RGCGCY 1 cut(s) 700
HgaI GACGC 2 cut(s) 452, 716
HhaI GCGC 2 cut(s) 445, 699
Hin1II CATG 2 cut(s) 419, 589
Hin6I GCGC 2 cut(s) 443, 697
HinP1I GCGC 2 cut(s) 443, 697
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 3 cut(s) 61, 265, 878
HphI GGTGA 3 cut(s) 185, 248, 610
Hpy166II GTNNAC 1 cut(s) 467
Hpy188I TCNGA 4 cut(s) 31, 264, 340, 887
Hpy188III TCNNGA 6 cut(s) 58, 129, 533, 586, 692, 776
Hpy8I GTNNAC 1 cut(s) 467
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 8 cut(s) 279, 285, 345, 433, 589, 800, 823, 863
HpyCH4III ACNGT 3 cut(s) 227, 236, 511
HpyCH4V TGCA 3 cut(s) 710, 749, 854
HpyF10VI GCNNNNNNNGC 6 cut(s) 104, 370, 482, 670, 749, 758
HpyF3I CTNAG 6 cut(s) 294, 339, 534, 624, 657, 693
Hsp92II CATG 2 cut(s) 419, 589
HspAI GCGC 2 cut(s) 443, 697
Kzo9I GATC 5 cut(s) 242, 331, 556, 688, 816
LmnI GCTCC 7 cut(s) 131, 490, 564, 661, 704, 832, 872
Lsp1109I GCAGC 2 cut(s) 94, 761
LweI GCATC 1 cut(s) 736
MabI ACCWGGT 1 cut(s) 469
MaeIII GTNAC 2 cut(s) 71, 83
MalI GATC 5 cut(s) 244, 333, 558, 690, 818
MboI GATC 5 cut(s) 242, 331, 556, 688, 816
MboII GAAGA 8 cut(s) 46, 129, 132, 135, 144, 296, 330, 460
MflI RGATCY 1 cut(s) 688
MluCI AATT 2 cut(s) 518, 731
MlyI GAGTC 2 cut(s) 259, 872
MmeI TCCRAC 3 cut(s) 54, 287, 726
MnlI CCTC 7 cut(s) 7, 35, 240, 540, 751, 772, 783
MseI TTAA 4 cut(s) 390, 396, 516, 579
MslI CAYNNNNRTG 1 cut(s) 237
MspA1I CMGCKG 1 cut(s) 752
MspR9I CCNGG 3 cut(s) 366, 471, 489
MvaI CCWGG 3 cut(s) 366, 471, 489
MwoI GCNNNNNNNGC 6 cut(s) 104, 370, 482, 670, 749, 758
NdeII GATC 5 cut(s) 242, 331, 556, 688, 816
NlaIII CATG 2 cut(s) 419, 589
NlaIV GGNNCC 1 cut(s) 690
NmuCI GTSAC 1 cut(s) 83
NspI RCATGY 1 cut(s) 419
PagI TCATGA 1 cut(s) 585
PciI ACATGT 1 cut(s) 415
PfeI GAWTC 1 cut(s) 61
PflFI GACNNNGTC 1 cut(s) 881
PfoI TCCNGGA 1 cut(s) 487
PkrI GCNGC 3 cut(s) 97, 109, 751
PleI GAGTC 2 cut(s) 259, 872
PpsI GAGTC 2 cut(s) 259, 872
PscI ACATGT 1 cut(s) 415
PsiI TTATAA 1 cut(s) 222
Psp6I CCWGG 3 cut(s) 364, 469, 487
PspGI CCWGG 3 cut(s) 364, 469, 487
PspN4I GGNNCC 1 cut(s) 690
PspPI GGNCC 3 cut(s) 227, 605, 788
PsuI RGATCY 1 cut(s) 688
PsyI GACNNNGTC 1 cut(s) 881
RseI CAYNNNNRTG 1 cut(s) 237
SaqAI TTAA 4 cut(s) 390, 396, 516, 579
SatI GCNGC 3 cut(s) 96, 108, 750
Sau3AI GATC 5 cut(s) 242, 331, 556, 688, 816
Sau96I GGNCC 3 cut(s) 227, 605, 788
SchI GAGTC 2 cut(s) 259, 872
ScrFI CCNGG 3 cut(s) 366, 471, 489
SexAI ACCWGGT 1 cut(s) 469
SfaNI GCATC 1 cut(s) 736
SinI GGWCC 3 cut(s) 227, 605, 788
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 1 cut(s) 76
SmoI CTYRAG 1 cut(s) 76
Sse9I AATT 2 cut(s) 518, 731
SsiI CCGC 4 cut(s) 81, 96, 743, 752
SspI AATATT 1 cut(s) 669
StyD4I CCNGG 3 cut(s) 364, 469, 487
TaaI ACNGT 3 cut(s) 227, 236, 511
TaqI TCGA 2 cut(s) 633, 819
TasI AATT 2 cut(s) 518, 731
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 1 cut(s) 61
Tru1I TTAA 4 cut(s) 390, 396, 516, 579
Tru9I TTAA 4 cut(s) 390, 396, 516, 579
TseFI GTSAC 1 cut(s) 83
TseI GCWGC 2 cut(s) 107, 749
Tsp45I GTSAC 1 cut(s) 83
TspDTI ATGAA 2 cut(s) 273, 837
TspGWI ACGGA 2 cut(s) 26, 108
Tth111I GACNNNGTC 1 cut(s) 881
VpaK11BI GGWCC 3 cut(s) 227, 605, 788
XapI RAATTY 1 cut(s) 518
XceI RCATGY 1 cut(s) 419
XcmI CCANNNNNNNNNTGG 2 cut(s) 212, 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.