Prupe.4G035500_v2.0.a1

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0007622
Physical Location & Seq
Forward (+)
1679844 .. 1683315
3472 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G035500.2

Sequence Viewer

Length: 789 bp
ATGACCATAGCTGACCAAAAGTCGTCGTCCGACGGAAAAGTCTGGGGCTTCTTCAAGCGGCCGTTTCGGCCGTCCGGGAACACCACCGTTACGACGTCGTCCACATCGTCGTCGCACAACGCTCCTCAACATAGCAACGGTCCTCAAGGCGACGGATCAAATCCACCGGCTACGAATTCGGTGTCGTCCGTCGCCAGGTCGCTGCTCCCCACTCGCCGTAGACTCAAGCTCGATCCCCACAATAAACTCTATTTTCCTTGTGAACCTGGAAAGCAGGTTAGGAGCGCCATTAGAATTAAAAACACAAGCAAGTCATATGTAGCTTTCAAGTTTCAAACAACTGCACCAAAAAGTTGTTTCATGCGTCCTCCAGGAGCTATTCTTAGTCCTGGCGAAAGCATTATAGCAACTGTATTCAAGTTTGTGGAGGTTCCGGAGAACAATGAAAAATTGGTGGATCAAAAGAGCAGGGTTAAATTTAAAATCATGAGCCTGAAGGTGAAAGGAGCAATGGACTATGTACCCGAGCTGTTTGATGAGCTGAAGGACCAAGTGGCAATAGAGCAGATCCTACGGGTTGTCTTTTTAGATCCAGAGCGTTCTACCCCGGCTATGGAAAAACTGAAGCGTCAATTGGCCGATGCTGATGCTGCGCTTGAGGAACGCAAGAAACCTCCAGAAGATGCTGGTCCGAAGATTATTGGAGAAGGGCTCGTGATAGATGAATGGAAGGAGAGGAGGGAAAGATATCTTGCTAGGCAGCAAGTGGAAGGGGTGGACTCGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

263

Amino Acids

29.19

Weight (kDa)

9.55

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 38
AatII GACGTC 1 cut(s) 98
Acc36I ACCTGC 1 cut(s) 265
AccI GTMKAC 1 cut(s) 220
AccIII TCCGGA 1 cut(s) 433
AciI CCGC 1 cut(s) 58
AclWI GGATC 5 cut(s) 163, 227, 465, 562, 584
AcoI YGGCCR 3 cut(s) 59, 68, 636
AcsI RAATTY 2 cut(s) 175, 476
AcuI CTGAAG 3 cut(s) 515, 563, 644
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 1 cut(s) 522
AfiI CCNNNNNNNGG 2 cut(s) 195, 613
AgsI TTSAA 4 cut(s) 55, 328, 335, 418
AhdI GACNNNNNGTC 1 cut(s) 19
AjnI CCWGG 4 cut(s) 194, 265, 370, 388
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
AluBI AGCT 6 cut(s) 11, 229, 323, 377, 529, 541
AluI AGCT 6 cut(s) 11, 229, 323, 377, 529, 541
AlwI GGATC 5 cut(s) 163, 227, 465, 562, 584
Ama87I CYCGRG 1 cut(s) 524
Aor13HI TCCGGA 1 cut(s) 433
AoxI GGCC 3 cut(s) 59, 68, 636
ApeKI GCWGC 3 cut(s) 202, 650, 760
ApoI RAATTY 2 cut(s) 175, 476
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AspLEI GCGC 2 cut(s) 287, 655
AspS9I GGNCC 3 cut(s) 140, 547, 689
AsuC2I CCSGG 2 cut(s) 76, 608
AsuHPI GGTGA 1 cut(s) 511
AvaI CYCGRG 1 cut(s) 524
AvaII GGWCC 3 cut(s) 140, 547, 689
BanII GRGCYC 1 cut(s) 714
BauI CACGAG 1 cut(s) 713
BbvI GCAGC 3 cut(s) 189, 637, 772
BceAI ACGGC 3 cut(s) 46, 55, 201
BcgI CGANNNNNNTGC 2 cut(s) 629, 663
BciT130I CCWGG 4 cut(s) 196, 267, 372, 390
BcnI CCSGG 2 cut(s) 76, 608
BfaI CTAG 1 cut(s) 756
BfoI RGCGCY 1 cut(s) 288
BfuAI ACCTGC 1 cut(s) 265
BglI GCCNNNNNGGC 1 cut(s) 67
BisI GCNGC 4 cut(s) 59, 203, 651, 761
BlsI GCNGC 4 cut(s) 60, 204, 652, 762
Bme1390I CCNGG 6 cut(s) 76, 196, 267, 372, 390, 608
Bme18I GGWCC 3 cut(s) 140, 547, 689
BmeRI GACNNNNNGTC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 524
BmgT120I GGNCC 3 cut(s) 140, 547, 689
BmiI GGNNCC 1 cut(s) 432
BmrFI CCNGG 6 cut(s) 76, 196, 267, 372, 390, 608
BmsI GCATC 3 cut(s) 631, 637, 673
BplI GAGNNNNNCTC 2 cut(s) 696, 728
BpmI CTGGAG 2 cut(s) 354, 660
BpuEI CTTGAG 3 cut(s) 129, 209, 677
BpuMI CCSGG 2 cut(s) 76, 608
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 606
BsaWI WCCGGW 1 cut(s) 433
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 613
Bse118I RCCGGY 1 cut(s) 166
Bse3DI GCAATG 1 cut(s) 516
BseAI TCCGGA 1 cut(s) 433
BseBI CCWGG 4 cut(s) 196, 267, 372, 390
BseDI CCNNGG 1 cut(s) 606
BseLI CCNNNNNNNGG 2 cut(s) 195, 613
BseMI GCAATG 1 cut(s) 516
BseRI GAGGAG 2 cut(s) 114, 751
BseX3I CGGCCG 2 cut(s) 59, 68
BseXI GCAGC 3 cut(s) 189, 637, 772
BsgI GTGCAG 1 cut(s) 327
Bsh1285I CGRYCG 2 cut(s) 62, 71
BshFI GGCC 3 cut(s) 61, 70, 638
BsiEI CGRYCG 2 cut(s) 62, 71
BsiHKCI CYCGRG 1 cut(s) 524
BsiSI CCGG 4 cut(s) 75, 167, 434, 608
BslI CCNNNNNNNGG 2 cut(s) 195, 613
BsnI GGCC 3 cut(s) 61, 70, 638
BsoBI CYCGRG 1 cut(s) 524
Bsp1286I GDGCHC 1 cut(s) 714
Bsp13I TCCGGA 1 cut(s) 433
Bsp143I GATC 5 cut(s) 155, 232, 457, 567, 589
BspACI CCGC 1 cut(s) 58
BspANI GGCC 3 cut(s) 61, 70, 638
BspEI TCCGGA 1 cut(s) 433
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 1 cut(s) 432
BspMI ACCTGC 1 cut(s) 265
BspPI GGATC 5 cut(s) 163, 227, 465, 562, 584
BsrDI GCAATG 1 cut(s) 516
BsrFI RCCGGY 1 cut(s) 166
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 606
BssMI GATC 5 cut(s) 155, 232, 457, 567, 589
BssNI GRCGYC 1 cut(s) 95
BssSI CACGAG 1 cut(s) 713
Bst2BI CACGAG 1 cut(s) 713
Bst2UI CCWGG 4 cut(s) 196, 267, 372, 390
Bst4CI ACNGT 3 cut(s) 88, 140, 412
BstACI GRCGYC 1 cut(s) 95
BstDEI CTNAG 1 cut(s) 383
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 2 cut(s) 287, 655
BstKTI GATC 5 cut(s) 158, 235, 460, 570, 592
BstMBI GATC 5 cut(s) 155, 232, 457, 567, 589
BstMCI CGRYCG 2 cut(s) 62, 71
BstMWI GCNNNNNNNGC 2 cut(s) 67, 650
BstNI CCWGG 4 cut(s) 196, 267, 372, 390
BstSCI CCNGG 6 cut(s) 74, 194, 265, 370, 388, 606
BstV1I GCAGC 3 cut(s) 189, 637, 772
BstX2I RGATCY 2 cut(s) 567, 589
BstYI RGATCY 2 cut(s) 567, 589
BstZI CGGCCG 2 cut(s) 59, 68
BsuRI GGCC 3 cut(s) 61, 70, 638
BveI ACCTGC 1 cut(s) 265
CciI TCATGA 1 cut(s) 486
CfoI GCGC 2 cut(s) 287, 655
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 3 cut(s) 140, 547, 689
CseI GACGC 2 cut(s) 353, 617
Csp6I GTAC 1 cut(s) 521
CviAII CATG 3 cut(s) 361, 487, 786
CviQI GTAC 1 cut(s) 521
DdeI CTNAG 1 cut(s) 383
DpnI GATC 5 cut(s) 157, 234, 459, 569, 591
DpnII GATC 5 cut(s) 155, 232, 457, 567, 589
DraI TTTAAA 1 cut(s) 481
DrdI GACNNNNNNGTC 1 cut(s) 38
DriI GACNNNNNGTC 1 cut(s) 19
DseDI GACNNNNNNGTC 1 cut(s) 38
EaeI YGGCCR 3 cut(s) 59, 68, 636
EagI CGGCCG 2 cut(s) 59, 68
Eam1105I GACNNNNNGTC 1 cut(s) 19
EclXI CGGCCG 2 cut(s) 59, 68
Eco24I GRGCYC 1 cut(s) 714
Eco32I GATATC 1 cut(s) 749
Eco47I GGWCC 3 cut(s) 140, 547, 689
Eco52I CGGCCG 2 cut(s) 59, 68
Eco57I CTGAAG 3 cut(s) 515, 563, 644
Eco88I CYCGRG 1 cut(s) 524
EcoRI GAATTC 1 cut(s) 175
EcoRII CCWGG 4 cut(s) 194, 265, 370, 388
EcoRV GATATC 1 cut(s) 749
EcoT38I GRGCYC 1 cut(s) 714
FaeI CATG 3 cut(s) 364, 490, 789
FatI CATG 3 cut(s) 360, 486, 785
FauNDI CATATG 1 cut(s) 316
FblI GTMKAC 1 cut(s) 220
Fnu4HI GCNGC 4 cut(s) 59, 203, 651, 761
FriOI GRGCYC 1 cut(s) 714
Fsp4HI GCNGC 4 cut(s) 59, 203, 651, 761
FspBI CTAG 1 cut(s) 756
GlaI GCGC 2 cut(s) 286, 654
GluI GCNGC 4 cut(s) 59, 203, 651, 761
GsuI CTGGAG 2 cut(s) 354, 660
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 3 cut(s) 61, 70, 638
HapII CCGG 4 cut(s) 75, 167, 434, 608
HgaI GACGC 2 cut(s) 353, 617
HhaI GCGC 2 cut(s) 287, 655
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 3 cut(s) 364, 490, 789
Hin6I GCGC 2 cut(s) 285, 653
HinP1I GCGC 2 cut(s) 285, 653
HinfI GANTC 2 cut(s) 222, 779
HpaII CCGG 4 cut(s) 75, 167, 434, 608
HphI GGTGA 1 cut(s) 511
Hpy166II GTNNAC 4 cut(s) 102, 221, 263, 778
Hpy188I TCNGA 2 cut(s) 31, 693
Hpy188III TCNNGA 5 cut(s) 434, 487, 593, 677, 715
Hpy8I GTNNAC 4 cut(s) 102, 221, 263, 778
Hpy99I CGWCG 8 cut(s) 28, 35, 97, 100, 112, 115, 155, 194
HpyAV CCTTC 5 cut(s) 490, 538, 701, 724, 764
HpyCH4III ACNGT 3 cut(s) 88, 140, 412
HpyCH4IV ACGT 1 cut(s) 95
HpyCH4V TGCA 1 cut(s) 344
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 650
HpyF3I CTNAG 1 cut(s) 383
HpySE526I ACGT 1 cut(s) 95
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 3 cut(s) 364, 490, 789
HspAI GCGC 2 cut(s) 285, 653
Kpn2I TCCGGA 1 cut(s) 433
Kzo9I GATC 5 cut(s) 155, 232, 457, 567, 589
LmnI GCTCC 5 cut(s) 127, 210, 282, 374, 506
Lsp1109I GCAGC 3 cut(s) 189, 637, 772
LweI GCATC 3 cut(s) 631, 637, 673
MaeI CTAG 1 cut(s) 756
MaeII ACGT 1 cut(s) 95
MaeIII GTNAC 1 cut(s) 88
MalI GATC 5 cut(s) 157, 234, 459, 569, 591
MboI GATC 5 cut(s) 155, 232, 457, 567, 589
MboII GAAGA 3 cut(s) 43, 692, 706
MfeI CAATTG 1 cut(s) 632
MflI RGATCY 2 cut(s) 567, 589
MhlI GDGCHC 1 cut(s) 714
MluCI AATT 5 cut(s) 175, 294, 449, 476, 632
MlyI GAGTC 2 cut(s) 216, 773
MmeI TCCRAC 1 cut(s) 54
MnlI CCTC 8 cut(s) 135, 153, 378, 421, 652, 684, 729, 732
MroI TCCGGA 1 cut(s) 433
MseI TTAA 3 cut(s) 297, 474, 480
MspI CCGG 4 cut(s) 75, 167, 434, 608
MspR9I CCNGG 6 cut(s) 76, 196, 267, 372, 390, 608
MunI CAATTG 1 cut(s) 632
MvaI CCWGG 4 cut(s) 196, 267, 372, 390
MwoI GCNNNNNNNGC 2 cut(s) 67, 650
NciI CCSGG 2 cut(s) 76, 608
NdeI CATATG 1 cut(s) 316
NdeII GATC 5 cut(s) 155, 232, 457, 567, 589
NlaIII CATG 3 cut(s) 364, 490, 789
NlaIV GGNNCC 1 cut(s) 432
NmeAIII GCCGAG 1 cut(s) 761
PagI TCATGA 1 cut(s) 486
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PflFI GACNNNGTC 1 cut(s) 97
PfoI TCCNGGA 2 cut(s) 74, 370
PkrI GCNGC 4 cut(s) 60, 204, 652, 762
PleI GAGTC 2 cut(s) 216, 773
PpsI GAGTC 2 cut(s) 216, 773
Psp6I CCWGG 4 cut(s) 194, 265, 370, 388
PspGI CCWGG 4 cut(s) 194, 265, 370, 388
PspN4I GGNNCC 1 cut(s) 432
PspPI GGNCC 3 cut(s) 140, 547, 689
PsuI RGATCY 2 cut(s) 567, 589
PsyI GACNNNGTC 1 cut(s) 97
RsaI GTAC 1 cut(s) 522
RsaNI GTAC 1 cut(s) 521
SaqAI TTAA 3 cut(s) 297, 474, 480
SatI GCNGC 4 cut(s) 59, 203, 651, 761
Sau3AI GATC 5 cut(s) 155, 232, 457, 567, 589
Sau96I GGNCC 3 cut(s) 140, 547, 689
SchI GAGTC 2 cut(s) 216, 773
ScrFI CCNGG 6 cut(s) 76, 196, 267, 372, 390, 608
SduI GDGCHC 1 cut(s) 714
SfaNI GCATC 3 cut(s) 631, 637, 673
SfiI GGCCNNNNNGGCC 1 cut(s) 67
SinI GGWCC 3 cut(s) 140, 547, 689
SmlI CTYRAG 3 cut(s) 144, 224, 656
SmoI CTYRAG 3 cut(s) 144, 224, 656
Sse9I AATT 5 cut(s) 175, 294, 449, 476, 632
SsiI CCGC 1 cut(s) 58
SspMI CTAG 1 cut(s) 756
StyD4I CCNGG 6 cut(s) 74, 194, 265, 370, 388, 606
TaaI ACNGT 3 cut(s) 88, 140, 412
TaiI ACGT 1 cut(s) 98
TaqI TCGA 1 cut(s) 231
TasI AATT 5 cut(s) 175, 294, 449, 476, 632
TauI GCSGC 1 cut(s) 61
Tru1I TTAA 3 cut(s) 297, 474, 480
Tru9I TTAA 3 cut(s) 297, 474, 480
TseI GCWGC 3 cut(s) 202, 650, 760
TspDTI ATGAA 3 cut(s) 349, 459, 738
TspGWI ACGGA 3 cut(s) 48, 168, 178
Tth111I GACNNNGTC 1 cut(s) 97
VpaK11BI GGWCC 3 cut(s) 140, 547, 689
XapI RAATTY 2 cut(s) 175, 476
XmiI GTMKAC 1 cut(s) 220
XspI CTAG 1 cut(s) 756
ZraI GACGTC 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.