RchiOBHm_Chr5g0006131

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007622
Physical Location & Seq
Forward (+)
3830840 .. 3834156
3317 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28727

Sequence Viewer

Length: 783 bp
ATGGCCATAGCTGACCACAAGTCTTCGTCGTCTTCGTCTGACGGAAAAGTCTGGGGCTTCTTCAAACGGCCGTTTCGACCGTCCGGTAACGCCGCCGCCACTACGACGTCGTCTTCTTCTATGTCGCACCACCAGCCGAACGGCGACGGATCGATTCCTCACGCCTCGAGTTCCGTGTCGTCGGTGGCCAGGTCGTTGCTTCCGACTCGTCGTAGACTGAAGCTCGATCCCTGCAATAAGCTATATTTTCCTTGTGAACCTGGGAAGCAGGTTAGGAGTGCCATTAGAATTAAAAACACAAGCAAGTCCCATGTAGCATTCAAGTTTCAAACGACTGCACCGAAAAGTTGTTTCATGCGTCCTCCTGGTGCTATTCTTAGTCCTGGTGAGAGTCTCATAGCCACAGTGTTCAAGTTTGTGGAGGTTCCTGAGAACAATGAAAAACTAGTGGATCAAAAGAGCCGAGTTAAATTTAAAATAATGAGCCTGAAGGTGAAGGGGCCAATGGACTATGTACCCGAGCTGTTTGATGAGCAGAAGGAACAAGTAGCAATAGAGCAGATACTACGGGTTGTCTTTCTGGATCCAGAGCGCTCTACCCCTGCTATGGAAAAATTGAAGCGCCAGTTGGCTGATGCTGATGCTGCGCTTGAAGCACGCAAGAAACCTCCAGAAGATGCTGGTCCAAAGATCATAGGAGAAGGTCTTGTCATAGATGAATGGAAGGAGCGGAGGGAAAGATATCTTGCTCGGCAGCAGGTTGAAGGGTTGGACTCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

260

Amino Acids

28.86

Weight (kDa)

9.59

Isoelectric Point (pI)

52.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 73 - 182 1.2e-22 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 47
AatII GACGTC 1 cut(s) 110
Acc36I ACCTGC 2 cut(s) 259, 748
AccBSI CCGCTC 1 cut(s) 730
AccI GTMKAC 1 cut(s) 214
AciI CCGC 3 cut(s) 93, 96, 730
AclWI GGATC 5 cut(s) 157, 221, 459, 578, 591
AcoI YGGCCR 3 cut(s) 3, 68, 186
AcsI RAATTY 1 cut(s) 470
AcuI CTGAAG 2 cut(s) 239, 509
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 1 cut(s) 516
AfeI AGCGCT 1 cut(s) 593
AfiI CCNNNNNNNGG 1 cut(s) 607
AgsI TTSAA 7 cut(s) 64, 322, 329, 412, 619, 653, 764
AhdI GACNNNNNGTC 1 cut(s) 19
AhlI ACTAGT 1 cut(s) 445
AjnI CCWGG 4 cut(s) 188, 259, 364, 382
AjuI GAANNNNNNNTTGG 2 cut(s) 611, 643
AluBI AGCT 4 cut(s) 11, 223, 241, 523
AluI AGCT 4 cut(s) 11, 223, 241, 523
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 5 cut(s) 157, 221, 459, 578, 591
Ama87I CYCGRG 2 cut(s) 166, 518
Aor51HI AGCGCT 1 cut(s) 593
AoxI GGCC 4 cut(s) 3, 68, 186, 500
ApeKI GCWGC 2 cut(s) 644, 754
ApoI RAATTY 1 cut(s) 470
AspLEI GCGC 3 cut(s) 594, 624, 649
AspS9I GGNCC 2 cut(s) 500, 683
AsuHPI GGTGA 2 cut(s) 398, 505
AvaI CYCGRG 2 cut(s) 166, 518
AvaII GGWCC 1 cut(s) 683
BalI TGGCCA 2 cut(s) 5, 188
BamHI GGATCC 1 cut(s) 583
BbsI GAAGAC 3 cut(s) 15, 24, 105
BbvI GCAGC 2 cut(s) 631, 766
BceAI ACGGC 3 cut(s) 55, 83, 157
BciT130I CCWGG 4 cut(s) 190, 261, 366, 384
BcoDI GTCTC 1 cut(s) 398
BcuI ACTAGT 1 cut(s) 445
BfaI CTAG 1 cut(s) 446
BfoI RGCGCY 2 cut(s) 595, 625
BfuAI ACCTGC 2 cut(s) 259, 748
BisI GCNGC 4 cut(s) 93, 96, 645, 755
BlsI GCNGC 4 cut(s) 94, 97, 646, 756
Bme1390I CCNGG 4 cut(s) 190, 261, 366, 384
Bme18I GGWCC 1 cut(s) 683
BmeRI GACNNNNNGTC 1 cut(s) 19
BmeT110I CYCGRG 2 cut(s) 166, 518
BmgT120I GGNCC 2 cut(s) 500, 683
BmiI GGNNCC 3 cut(s) 426, 501, 585
BmrFI CCNGG 4 cut(s) 190, 261, 366, 384
BmsI GCATC 3 cut(s) 625, 631, 667
BpiI GAAGAC 3 cut(s) 15, 24, 105
BpmI CTGGAG 1 cut(s) 654
Bsa29I ATCGAT 1 cut(s) 152
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 260
BsaWI WCCGGW 1 cut(s) 83
Bsc4I CCNNNNNNNGG 1 cut(s) 607
Bse1I ACTGG 1 cut(s) 625
BseBI CCWGG 4 cut(s) 190, 261, 366, 384
BseCI ATCGAT 1 cut(s) 152
BseDI CCNNGG 1 cut(s) 260
BseLI CCNNNNNNNGG 1 cut(s) 607
BseMII CTCAG 1 cut(s) 420
BseNI ACTGG 1 cut(s) 625
BseX3I CGGCCG 1 cut(s) 68
BseXI GCAGC 2 cut(s) 631, 766
BsgI GTGCAG 1 cut(s) 321
Bsh1285I CGRYCG 2 cut(s) 71, 80
BshFI GGCC 4 cut(s) 5, 70, 188, 502
BshVI ATCGAT 1 cut(s) 152
BsiEI CGRYCG 2 cut(s) 71, 80
BsiHKCI CYCGRG 2 cut(s) 166, 518
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 1 cut(s) 607
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 292
BsmI GAATGC 1 cut(s) 317
BsnI GGCC 4 cut(s) 5, 70, 188, 502
BsoBI CYCGRG 2 cut(s) 166, 518
Bsp143I GATC 5 cut(s) 149, 226, 451, 583, 690
BspACI CCGC 3 cut(s) 93, 96, 730
BspANI GGCC 4 cut(s) 5, 70, 188, 502
BspCNI CTCAG 1 cut(s) 421
BspDI ATCGAT 1 cut(s) 152
BspLI GGNNCC 3 cut(s) 426, 501, 585
BspMI ACCTGC 2 cut(s) 259, 748
BspPI GGATC 5 cut(s) 157, 221, 459, 578, 591
BsrBI CCGCTC 1 cut(s) 730
BsrI ACTGG 1 cut(s) 625
BssECI CCNNGG 1 cut(s) 260
BssMI GATC 5 cut(s) 149, 226, 451, 583, 690
BssNI GRCGYC 1 cut(s) 107
Bst2UI CCWGG 4 cut(s) 190, 261, 366, 384
Bst4CI ACNGT 2 cut(s) 81, 406
BstACI GRCGYC 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 658
BstDEI CTNAG 2 cut(s) 377, 429
BstH2I RGCGCY 2 cut(s) 595, 625
BstHHI GCGC 3 cut(s) 594, 624, 649
BstKTI GATC 5 cut(s) 152, 229, 454, 586, 693
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 5 cut(s) 149, 226, 451, 583, 690
BstMCI CGRYCG 2 cut(s) 71, 80
BstMWI GCNNNNNNNGC 3 cut(s) 133, 644, 653
BstNI CCWGG 4 cut(s) 190, 261, 366, 384
BstSCI CCNGG 4 cut(s) 188, 259, 364, 382
BstV1I GCAGC 2 cut(s) 631, 766
BstV2I GAAGAC 3 cut(s) 15, 24, 105
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
BstZI CGGCCG 1 cut(s) 68
Bsu15I ATCGAT 1 cut(s) 152
BsuRI GGCC 4 cut(s) 5, 70, 188, 502
BsuTUI ATCGAT 1 cut(s) 152
BtsIMutI CAGTG 1 cut(s) 411
BveI ACCTGC 2 cut(s) 259, 748
Cac8I GCNNGC 1 cut(s) 658
CfoI GCGC 3 cut(s) 594, 624, 649
Cfr13I GGNCC 2 cut(s) 500, 683
ClaI ATCGAT 1 cut(s) 152
CseI GACGC 1 cut(s) 347
Csp6I GTAC 1 cut(s) 515
CviAII CATG 3 cut(s) 311, 355, 780
CviQI GTAC 1 cut(s) 515
DdeI CTNAG 2 cut(s) 377, 429
DpnI GATC 5 cut(s) 151, 228, 453, 585, 692
DpnII GATC 5 cut(s) 149, 226, 451, 583, 690
DraI TTTAAA 1 cut(s) 475
DrdI GACNNNNNNGTC 1 cut(s) 47
DriI GACNNNNNGTC 1 cut(s) 19
DseDI GACNNNNNNGTC 1 cut(s) 47
EaeI YGGCCR 3 cut(s) 3, 68, 186
EagI CGGCCG 1 cut(s) 68
Eam1105I GACNNNNNGTC 1 cut(s) 19
EclXI CGGCCG 1 cut(s) 68
Eco32I GATATC 1 cut(s) 743
Eco47I GGWCC 1 cut(s) 683
Eco47III AGCGCT 1 cut(s) 593
Eco52I CGGCCG 1 cut(s) 68
Eco57I CTGAAG 2 cut(s) 239, 509
Eco88I CYCGRG 2 cut(s) 166, 518
EcoRII CCWGG 4 cut(s) 188, 259, 364, 382
EcoRV GATATC 1 cut(s) 743
FaeI CATG 3 cut(s) 314, 358, 783
FaqI GGGAC 1 cut(s) 292
FatI CATG 3 cut(s) 310, 354, 779
FblI GTMKAC 1 cut(s) 214
Fnu4HI GCNGC 4 cut(s) 93, 96, 645, 755
Fsp4HI GCNGC 4 cut(s) 93, 96, 645, 755
FspBI CTAG 1 cut(s) 446
GlaI GCGC 3 cut(s) 593, 623, 648
GluI GCNGC 4 cut(s) 93, 96, 645, 755
GsuI CTGGAG 1 cut(s) 654
HaeII RGCGCY 2 cut(s) 595, 625
HaeIII GGCC 4 cut(s) 5, 70, 188, 502
HapII CCGG 1 cut(s) 84
HgaI GACGC 1 cut(s) 347
HhaI GCGC 3 cut(s) 594, 624, 649
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 3 cut(s) 314, 358, 783
Hin6I GCGC 3 cut(s) 592, 622, 647
HinP1I GCGC 3 cut(s) 592, 622, 647
HinfI GANTC 4 cut(s) 154, 205, 391, 773
HpaII CCGG 1 cut(s) 84
HphI GGTGA 2 cut(s) 398, 505
Hpy166II GTNNAC 2 cut(s) 215, 257
Hpy188I TCNGA 2 cut(s) 40, 204
Hpy188III TCNNGA 4 cut(s) 428, 581, 587, 671
Hpy8I GTNNAC 2 cut(s) 215, 257
Hpy99I CGWCG 6 cut(s) 31, 109, 112, 149, 184, 213
HpyAV CCTTC 6 cut(s) 484, 490, 532, 695, 718, 758
HpyCH4III ACNGT 2 cut(s) 81, 406
HpyCH4IV ACGT 1 cut(s) 107
HpyCH4V TGCA 3 cut(s) 234, 338, 779
HpyF10VI GCNNNNNNNGC 3 cut(s) 133, 644, 653
HpyF3I CTNAG 2 cut(s) 377, 429
HpySE526I ACGT 1 cut(s) 107
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 3 cut(s) 314, 358, 783
HspAI GCGC 3 cut(s) 592, 622, 647
Kzo9I GATC 5 cut(s) 149, 226, 451, 583, 690
LmnI GCTCC 1 cut(s) 727
Lsp1109I GCAGC 2 cut(s) 631, 766
LweI GCATC 3 cut(s) 625, 631, 667
MaeI CTAG 1 cut(s) 446
MaeII ACGT 1 cut(s) 107
MaeIII GTNAC 1 cut(s) 86
MalI GATC 5 cut(s) 151, 228, 453, 585, 692
MbiI CCGCTC 1 cut(s) 730
MboI GATC 5 cut(s) 149, 226, 451, 583, 690
MboII GAAGA 6 cut(s) 15, 24, 52, 105, 108, 686
MflI RGATCY 1 cut(s) 583
MlsI TGGCCA 2 cut(s) 5, 188
MluCI AATT 3 cut(s) 288, 470, 614
MluNI TGGCCA 2 cut(s) 5, 188
MlyI GAGTC 3 cut(s) 199, 400, 767
MmeI TCCRAC 2 cut(s) 227, 750
MnlI CCTC 6 cut(s) 168, 175, 372, 415, 678, 726
Mox20I TGGCCA 2 cut(s) 5, 188
MscI TGGCCA 2 cut(s) 5, 188
MseI TTAA 3 cut(s) 291, 468, 474
Msp20I TGGCCA 2 cut(s) 5, 188
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 4 cut(s) 190, 261, 366, 384
Mva1269I GAATGC 1 cut(s) 317
MvaI CCWGG 4 cut(s) 190, 261, 366, 384
MwoI GCNNNNNNNGC 3 cut(s) 133, 644, 653
NdeII GATC 5 cut(s) 149, 226, 451, 583, 690
NlaIII CATG 3 cut(s) 314, 358, 783
NlaIV GGNNCC 3 cut(s) 426, 501, 585
NmeAIII GCCGAG 2 cut(s) 488, 730
PaeR7I CTCGAG 1 cut(s) 166
PcsI WCGNNNNNNNCGW 4 cut(s) 32, 73, 200, 338
PctI GAATGC 1 cut(s) 317
PfeI GAWTC 1 cut(s) 154
PflFI GACNNNGTC 1 cut(s) 109
PkrI GCNGC 4 cut(s) 94, 97, 646, 756
PleI GAGTC 3 cut(s) 199, 399, 767
PpsI GAGTC 3 cut(s) 199, 399, 767
Psp6I CCWGG 4 cut(s) 188, 259, 364, 382
PspGI CCWGG 4 cut(s) 188, 259, 364, 382
PspN4I GGNNCC 3 cut(s) 426, 501, 585
PspPI GGNCC 2 cut(s) 500, 683
PspXI VCTCGAGB 1 cut(s) 166
PsuI RGATCY 1 cut(s) 583
PsyI GACNNNGTC 1 cut(s) 109
RsaI GTAC 1 cut(s) 516
RsaNI GTAC 1 cut(s) 515
SaqAI TTAA 3 cut(s) 291, 468, 474
SatI GCNGC 4 cut(s) 93, 96, 645, 755
Sau3AI GATC 5 cut(s) 149, 226, 451, 583, 690
Sau96I GGNCC 2 cut(s) 500, 683
SchI GAGTC 3 cut(s) 199, 400, 767
ScrFI CCNGG 4 cut(s) 190, 261, 366, 384
SfaNI GCATC 3 cut(s) 625, 631, 667
Sfr274I CTCGAG 1 cut(s) 166
SinI GGWCC 1 cut(s) 683
SlaI CTCGAG 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
SpeI ACTAGT 1 cut(s) 445
Sse9I AATT 3 cut(s) 288, 470, 614
SsiI CCGC 3 cut(s) 93, 96, 730
SspMI CTAG 1 cut(s) 446
StyD4I CCNGG 4 cut(s) 188, 259, 364, 382
TaaI ACNGT 2 cut(s) 81, 406
TaiI ACGT 1 cut(s) 110
TaqI TCGA 4 cut(s) 76, 152, 167, 225
TasI AATT 3 cut(s) 288, 470, 614
TauI GCSGC 2 cut(s) 95, 98
TfiI GAWTC 1 cut(s) 154
Tru1I TTAA 3 cut(s) 291, 468, 474
Tru9I TTAA 3 cut(s) 291, 468, 474
TscAI CASTG 1 cut(s) 411
TseI GCWGC 2 cut(s) 644, 754
TspDTI ATGAA 3 cut(s) 343, 453, 732
TspGWI ACGGA 3 cut(s) 57, 162, 163
TspRI CASTG 1 cut(s) 411
Tth111I GACNNNGTC 1 cut(s) 109
VpaK11BI GGWCC 1 cut(s) 683
XapI RAATTY 1 cut(s) 470
XhoI CTCGAG 1 cut(s) 166
XmiI GTMKAC 1 cut(s) 214
XspI CTAG 1 cut(s) 446
ZraI GACGTC 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.