RLG00000031380

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0007622
Physical Location & Seq
Reverse (-)
4520867 .. 4523332
2466 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031380

Sequence Viewer

Length: 783 bp
ATGGCCATAGCTGACCACAAGTCTTCGTCGTCTTCGTCTGACGGAAAAGTCTGGGGCTTCTTCAAACGGCCGTTTCGACCGTCCGGTAACGCCGCCGCCACTACGACGTCCTCTTCTTCTATGTCGCACCACCAGCCGAACGGCGACGGATCAATTCCTCACGCCTCGAGTTCCGTGTCGTCGGTAGCCAGGTCGCTGCTTCCGACTCGTCGTAGACTGAAGCTCGATCCCTGCAATAAGCTATATTTTCCTTGTGAACCTGGGAAGCAGGTTAGGAGTGCCATTAGAATTAAAAACACAAGCAAGTCCCATGTAGCGTTCAAGTTTCAAACGACTGCACCGAAAAGTTGTTTCATGCGTCCTCCTGGTGCTATTCTTAGTCCTGGTGAGAGTCTCATAGCCACAGTGTTCAAGTTTGTGGAGGTTCCTGAGAACAATGAAAAACTAGTGGATCAAAAGAGCCGAGTTAAATTTAAAATAATGAGCCTGAAGGTGAAGGGGCCAATGGACTATGTACCTGAGCTGTTTGAAGAGCAGAAGGAACAAGTAGCAATAGAGCAGATACTACGGGTTGTCTTTCTGGATCCAGAGCGCTCTACCCCTGCTATGGAAAAATTGAAGCGCCAGTTGGCTGATGCTGATGCTGCGCTTGAAGCACGCAAGAAACCTCCAGAAGATGCTGGTCCAAAGATCATAGGTGAAGGTCTTGTCATAGATGAATGGAAGGAGCGGAGGGAAAGATATCTTGCTCGGCAGCAGGTTGAAGGGTTGGACTCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

261

Amino Acids

28.88

Weight (kDa)

9.59

Isoelectric Point (pI)

53.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 73 - 182 1.1e-22 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 47
AatII GACGTC 1 cut(s) 110
Acc36I ACCTGC 2 cut(s) 259, 748
AccBSI CCGCTC 1 cut(s) 730
AccI GTMKAC 1 cut(s) 214
AciI CCGC 3 cut(s) 93, 96, 730
AclWI GGATC 5 cut(s) 157, 221, 459, 578, 591
AcoI YGGCCR 2 cut(s) 3, 68
AcsI RAATTY 1 cut(s) 470
AcuI CTGAAG 2 cut(s) 239, 509
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 1 cut(s) 516
AfeI AGCGCT 1 cut(s) 593
AfiI CCNNNNNNNGG 1 cut(s) 607
AgsI TTSAA 8 cut(s) 64, 322, 329, 412, 530, 619, 653, 764
AhdI GACNNNNNGTC 1 cut(s) 19
AhlI ACTAGT 1 cut(s) 445
AjnI CCWGG 4 cut(s) 188, 259, 364, 382
AjuI GAANNNNNNNTTGG 2 cut(s) 611, 643
AluBI AGCT 4 cut(s) 11, 223, 241, 523
AluI AGCT 4 cut(s) 11, 223, 241, 523
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 5 cut(s) 157, 221, 459, 578, 591
Ama87I CYCGRG 1 cut(s) 166
Aor51HI AGCGCT 1 cut(s) 593
AoxI GGCC 3 cut(s) 3, 68, 500
ApeKI GCWGC 3 cut(s) 196, 644, 754
ApoI RAATTY 1 cut(s) 470
AspLEI GCGC 3 cut(s) 594, 624, 649
AspS9I GGNCC 2 cut(s) 500, 683
AsuHPI GGTGA 3 cut(s) 398, 505, 710
AvaI CYCGRG 1 cut(s) 166
AvaII GGWCC 1 cut(s) 683
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 583
BbsI GAAGAC 2 cut(s) 15, 24
BbvI GCAGC 3 cut(s) 183, 631, 766
BceAI ACGGC 3 cut(s) 55, 83, 157
BciT130I CCWGG 4 cut(s) 190, 261, 366, 384
BcoDI GTCTC 1 cut(s) 398
BcuI ACTAGT 1 cut(s) 445
BfaI CTAG 1 cut(s) 446
BfoI RGCGCY 2 cut(s) 595, 625
BfuAI ACCTGC 2 cut(s) 259, 748
BisI GCNGC 5 cut(s) 93, 96, 197, 645, 755
BlsI GCNGC 5 cut(s) 94, 97, 198, 646, 756
Bme1390I CCNGG 4 cut(s) 190, 261, 366, 384
Bme18I GGWCC 1 cut(s) 683
BmeRI GACNNNNNGTC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 166
BmgT120I GGNCC 2 cut(s) 500, 683
BmiI GGNNCC 3 cut(s) 426, 501, 585
BmrFI CCNGG 4 cut(s) 190, 261, 366, 384
BmsI GCATC 3 cut(s) 625, 631, 667
BpiI GAAGAC 2 cut(s) 15, 24
BpmI CTGGAG 1 cut(s) 654
Bpu10I CCTNAGC 1 cut(s) 519
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 260
BsaWI WCCGGW 1 cut(s) 83
Bsc4I CCNNNNNNNGG 1 cut(s) 607
Bse1I ACTGG 1 cut(s) 625
BseBI CCWGG 4 cut(s) 190, 261, 366, 384
BseDI CCNNGG 1 cut(s) 260
BseLI CCNNNNNNNGG 1 cut(s) 607
BseMII CTCAG 2 cut(s) 420, 510
BseNI ACTGG 1 cut(s) 625
BseX3I CGGCCG 1 cut(s) 68
BseXI GCAGC 3 cut(s) 183, 631, 766
BsgI GTGCAG 1 cut(s) 321
Bsh1285I CGRYCG 2 cut(s) 71, 80
BshFI GGCC 3 cut(s) 5, 70, 502
BsiEI CGRYCG 2 cut(s) 71, 80
BsiHKCI CYCGRG 1 cut(s) 166
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 1 cut(s) 607
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 292
BsnI GGCC 3 cut(s) 5, 70, 502
BsoBI CYCGRG 1 cut(s) 166
Bsp143I GATC 5 cut(s) 149, 226, 451, 583, 690
BspACI CCGC 3 cut(s) 93, 96, 730
BspANI GGCC 3 cut(s) 5, 70, 502
BspCNI CTCAG 2 cut(s) 421, 511
BspLI GGNNCC 3 cut(s) 426, 501, 585
BspMI ACCTGC 2 cut(s) 259, 748
BspPI GGATC 5 cut(s) 157, 221, 459, 578, 591
BspQI GCTCTTC 1 cut(s) 525
BsrBI CCGCTC 1 cut(s) 730
BsrI ACTGG 1 cut(s) 625
BssECI CCNNGG 1 cut(s) 260
BssMI GATC 5 cut(s) 149, 226, 451, 583, 690
BssNI GRCGYC 1 cut(s) 107
Bst2UI CCWGG 4 cut(s) 190, 261, 366, 384
Bst4CI ACNGT 2 cut(s) 81, 406
Bst6I CTCTTC 2 cut(s) 118, 525
BstACI GRCGYC 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 658
BstDEI CTNAG 3 cut(s) 377, 429, 519
BstH2I RGCGCY 2 cut(s) 595, 625
BstHHI GCGC 3 cut(s) 594, 624, 649
BstKTI GATC 5 cut(s) 152, 229, 454, 586, 693
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 5 cut(s) 149, 226, 451, 583, 690
BstMCI CGRYCG 2 cut(s) 71, 80
BstMWI GCNNNNNNNGC 3 cut(s) 133, 644, 653
BstNI CCWGG 4 cut(s) 190, 261, 366, 384
BstSCI CCNGG 4 cut(s) 188, 259, 364, 382
BstV1I GCAGC 3 cut(s) 183, 631, 766
BstV2I GAAGAC 2 cut(s) 15, 24
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
BstZI CGGCCG 1 cut(s) 68
BsuRI GGCC 3 cut(s) 5, 70, 502
BtsIMutI CAGTG 1 cut(s) 411
BveI ACCTGC 2 cut(s) 259, 748
Cac8I GCNNGC 1 cut(s) 658
CfoI GCGC 3 cut(s) 594, 624, 649
Cfr13I GGNCC 2 cut(s) 500, 683
CseI GACGC 1 cut(s) 347
Csp6I GTAC 1 cut(s) 515
CviAII CATG 3 cut(s) 311, 355, 780
CviQI GTAC 1 cut(s) 515
DdeI CTNAG 3 cut(s) 377, 429, 519
DpnI GATC 5 cut(s) 151, 228, 453, 585, 692
DpnII GATC 5 cut(s) 149, 226, 451, 583, 690
DraI TTTAAA 1 cut(s) 475
DrdI GACNNNNNNGTC 1 cut(s) 47
DriI GACNNNNNGTC 1 cut(s) 19
DseDI GACNNNNNNGTC 1 cut(s) 47
EaeI YGGCCR 2 cut(s) 3, 68
EagI CGGCCG 1 cut(s) 68
Eam1104I CTCTTC 2 cut(s) 118, 525
Eam1105I GACNNNNNGTC 1 cut(s) 19
EarI CTCTTC 2 cut(s) 118, 525
EclXI CGGCCG 1 cut(s) 68
Eco32I GATATC 1 cut(s) 743
Eco47I GGWCC 1 cut(s) 683
Eco47III AGCGCT 1 cut(s) 593
Eco52I CGGCCG 1 cut(s) 68
Eco57I CTGAAG 2 cut(s) 239, 509
Eco88I CYCGRG 1 cut(s) 166
EcoRII CCWGG 4 cut(s) 188, 259, 364, 382
EcoRV GATATC 1 cut(s) 743
FaeI CATG 3 cut(s) 314, 358, 783
FaqI GGGAC 1 cut(s) 292
FatI CATG 3 cut(s) 310, 354, 779
FblI GTMKAC 1 cut(s) 214
Fnu4HI GCNGC 5 cut(s) 93, 96, 197, 645, 755
Fsp4HI GCNGC 5 cut(s) 93, 96, 197, 645, 755
FspBI CTAG 1 cut(s) 446
GlaI GCGC 3 cut(s) 593, 623, 648
GluI GCNGC 5 cut(s) 93, 96, 197, 645, 755
GsuI CTGGAG 1 cut(s) 654
HaeII RGCGCY 2 cut(s) 595, 625
HaeIII GGCC 3 cut(s) 5, 70, 502
HapII CCGG 1 cut(s) 84
HgaI GACGC 1 cut(s) 347
HhaI GCGC 3 cut(s) 594, 624, 649
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 3 cut(s) 314, 358, 783
Hin6I GCGC 3 cut(s) 592, 622, 647
HinP1I GCGC 3 cut(s) 592, 622, 647
HinfI GANTC 3 cut(s) 205, 391, 773
HpaII CCGG 1 cut(s) 84
HphI GGTGA 3 cut(s) 398, 505, 710
Hpy166II GTNNAC 2 cut(s) 215, 257
Hpy188I TCNGA 2 cut(s) 40, 204
Hpy188III TCNNGA 4 cut(s) 428, 581, 587, 671
Hpy8I GTNNAC 2 cut(s) 215, 257
Hpy99I CGWCG 5 cut(s) 31, 109, 149, 184, 213
HpyAV CCTTC 6 cut(s) 484, 490, 532, 695, 718, 758
HpyCH4III ACNGT 2 cut(s) 81, 406
HpyCH4IV ACGT 1 cut(s) 107
HpyCH4V TGCA 3 cut(s) 234, 338, 779
HpyF10VI GCNNNNNNNGC 3 cut(s) 133, 644, 653
HpyF3I CTNAG 3 cut(s) 377, 429, 519
HpySE526I ACGT 1 cut(s) 107
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 3 cut(s) 314, 358, 783
HspAI GCGC 3 cut(s) 592, 622, 647
Kzo9I GATC 5 cut(s) 149, 226, 451, 583, 690
LguI GCTCTTC 1 cut(s) 525
LmnI GCTCC 1 cut(s) 727
Lsp1109I GCAGC 3 cut(s) 183, 631, 766
LweI GCATC 3 cut(s) 625, 631, 667
MaeI CTAG 1 cut(s) 446
MaeII ACGT 1 cut(s) 107
MaeIII GTNAC 1 cut(s) 86
MalI GATC 5 cut(s) 151, 228, 453, 585, 692
MbiI CCGCTC 1 cut(s) 730
MboI GATC 5 cut(s) 149, 226, 451, 583, 690
MboII GAAGA 7 cut(s) 15, 24, 52, 105, 108, 542, 686
MflI RGATCY 1 cut(s) 583
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 153, 288, 470, 614
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 199, 400, 767
MmeI TCCRAC 2 cut(s) 227, 750
MnlI CCTC 7 cut(s) 121, 168, 175, 372, 415, 678, 726
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 291, 468, 474
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 4 cut(s) 190, 261, 366, 384
MvaI CCWGG 4 cut(s) 190, 261, 366, 384
MwoI GCNNNNNNNGC 3 cut(s) 133, 644, 653
NdeII GATC 5 cut(s) 149, 226, 451, 583, 690
NlaIII CATG 3 cut(s) 314, 358, 783
NlaIV GGNNCC 3 cut(s) 426, 501, 585
NmeAIII GCCGAG 2 cut(s) 488, 730
PaeR7I CTCGAG 1 cut(s) 166
PciSI GCTCTTC 1 cut(s) 525
PcsI WCGNNNNNNNCGW 4 cut(s) 32, 73, 200, 338
PkrI GCNGC 5 cut(s) 94, 97, 198, 646, 756
PleI GAGTC 3 cut(s) 199, 399, 767
PpsI GAGTC 3 cut(s) 199, 399, 767
Psp6I CCWGG 4 cut(s) 188, 259, 364, 382
PspGI CCWGG 4 cut(s) 188, 259, 364, 382
PspN4I GGNNCC 3 cut(s) 426, 501, 585
PspPI GGNCC 2 cut(s) 500, 683
PspXI VCTCGAGB 1 cut(s) 166
PsuI RGATCY 1 cut(s) 583
RsaI GTAC 1 cut(s) 516
RsaNI GTAC 1 cut(s) 515
SapI GCTCTTC 1 cut(s) 525
SaqAI TTAA 3 cut(s) 291, 468, 474
SatI GCNGC 5 cut(s) 93, 96, 197, 645, 755
Sau3AI GATC 5 cut(s) 149, 226, 451, 583, 690
Sau96I GGNCC 2 cut(s) 500, 683
SchI GAGTC 3 cut(s) 199, 400, 767
ScrFI CCNGG 4 cut(s) 190, 261, 366, 384
SfaNI GCATC 3 cut(s) 625, 631, 667
Sfr274I CTCGAG 1 cut(s) 166
SinI GGWCC 1 cut(s) 683
SlaI CTCGAG 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
SpeI ACTAGT 1 cut(s) 445
Sse9I AATT 4 cut(s) 153, 288, 470, 614
SsiI CCGC 3 cut(s) 93, 96, 730
SspMI CTAG 1 cut(s) 446
StyD4I CCNGG 4 cut(s) 188, 259, 364, 382
TaaI ACNGT 2 cut(s) 81, 406
TaiI ACGT 1 cut(s) 110
TaqI TCGA 3 cut(s) 76, 167, 225
TasI AATT 4 cut(s) 153, 288, 470, 614
TauI GCSGC 2 cut(s) 95, 98
Tru1I TTAA 3 cut(s) 291, 468, 474
Tru9I TTAA 3 cut(s) 291, 468, 474
TscAI CASTG 1 cut(s) 411
TseI GCWGC 3 cut(s) 196, 644, 754
TspDTI ATGAA 3 cut(s) 343, 453, 732
TspGWI ACGGA 3 cut(s) 57, 162, 163
TspRI CASTG 1 cut(s) 411
VpaK11BI GGWCC 1 cut(s) 683
XapI RAATTY 1 cut(s) 470
XhoI CTCGAG 1 cut(s) 166
XmiI GTMKAC 1 cut(s) 214
XspI CTAG 1 cut(s) 446
ZraI GACGTC 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.