MD05G1326200.v1.1

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007622
Physical Location & Seq
Reverse (-)
45236905 .. 45239467
2563 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1326200.v1.1.491

Sequence Viewer

Length: 795 bp
ATGGCCATCCCTGACCAAAAGTCGTCGTCGTCTTCGTCCGACGGAAAAGTCTGGGGCTTCTTCAAGCGGCCGTTTCGGACGTCCGGGAACGCCGCCGTTACGACGTCGTCCTCGTCGTCGCACAACGCTCCCCACCAGCATAGTAACGCTCACGGCGACGGATCGAATCAAAACGCCGCGAATTCGGTGTCGTCCGTCGCCAGGTCGCTGCTTCCGACTCGCCGTAGGCTCAAGCTCGATCCCCACAATAAACTCTATTTTCCTTGTGAACCCGGGAAGCAGGTTAGGAGTGCCATTAGAATTAAAAACACCAGCAAATCCTTTGTAGCATTCAAGTTCCAAACAACTGCACCGAAAAGTTGTTTCATGCGTCCTCCAGGAGCCATTCTTAGTCCTGGCGAGAGTCTTATAGCGACTGTATTCAAGTTTGTTGAGGTTCCCGAGAACAATGAAAAACTAGTGGATCAAAAGAGCAGAGTTAAGTTTAAAATCATGAGCTTGAAGGTGAAGGGACCAATGGAGTATGTACCTGAGCTGTTTGATGAGCAAAAGGAGCAAGTGGCCGTAGAGCAGATACTACGGGTTGTCTTTCTAGATCCAGAGCGTTCTACTCCTGCTATGGAAAAACTGAAGCGTCAGTTAGCTGATGCTGATGCTGCGCTTGAGGCGAGCAAGCAACCTGCGGAAGATGCTGGTCCAAAGATTATCGGAGAAGGACTTGTCATAGATGAATGGAAGGAGAGGAGGGAAAGATATCTTGCTCGGCAGCAAGGTGAAGGCGTGGACTCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

265

Amino Acids

29.21

Weight (kDa)

9.52

Isoelectric Point (pI)

47.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 77 - 187 2.9e-21 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 47
AatII GACGTC 2 cut(s) 83, 107
Acc36I ACCTGC 2 cut(s) 271, 688
AccII CGCG 1 cut(s) 179
AciI CCGC 4 cut(s) 67, 93, 177, 683
AclWI GGATC 4 cut(s) 169, 233, 471, 590
AcoI YGGCCR 3 cut(s) 3, 68, 561
AcsI RAATTY 1 cut(s) 181
AcuI CTGAAG 1 cut(s) 650
AcyI GRCGYC 2 cut(s) 80, 104
AfaI GTAC 1 cut(s) 528
AfiI CCNNNNNNNGG 1 cut(s) 201
AgsI TTSAA 4 cut(s) 64, 334, 424, 502
AhdI GACNNNNNGTC 1 cut(s) 19
AhlI ACTAGT 1 cut(s) 457
AjnI CCWGG 3 cut(s) 200, 376, 394
AluBI AGCT 4 cut(s) 235, 498, 535, 644
AluI AGCT 4 cut(s) 235, 498, 535, 644
AlwI GGATC 4 cut(s) 169, 233, 471, 590
Ama87I CYCGRG 2 cut(s) 272, 440
AoxI GGCC 3 cut(s) 3, 68, 561
ApeKI GCWGC 3 cut(s) 208, 656, 766
ApoI RAATTY 1 cut(s) 181
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AspLEI GCGC 1 cut(s) 661
AspS9I GGNCC 2 cut(s) 512, 695
AsuC2I CCSGG 3 cut(s) 85, 273, 274
AsuHPI GGTGA 2 cut(s) 517, 785
AvaI CYCGRG 2 cut(s) 272, 440
AvaII GGWCC 2 cut(s) 512, 695
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 24
BbvI GCAGC 3 cut(s) 195, 643, 778
BccI CCATC 1 cut(s) 14
BceAI ACGGC 5 cut(s) 55, 80, 169, 207, 548
BciT130I CCWGG 3 cut(s) 202, 378, 396
BcnI CCSGG 3 cut(s) 85, 273, 274
BcuI ACTAGT 1 cut(s) 457
BfaI CTAG 2 cut(s) 458, 593
BfuAI ACCTGC 2 cut(s) 271, 688
BisI GCNGC 6 cut(s) 68, 93, 177, 209, 657, 767
BlsI GCNGC 6 cut(s) 69, 94, 178, 210, 658, 768
Bme1390I CCNGG 6 cut(s) 85, 202, 273, 274, 378, 396
Bme18I GGWCC 2 cut(s) 512, 695
BmeRI GACNNNNNGTC 1 cut(s) 19
BmeT110I CYCGRG 2 cut(s) 272, 440
BmgT120I GGNCC 2 cut(s) 512, 695
BmiI GGNNCC 3 cut(s) 382, 438, 513
BmrFI CCNGG 6 cut(s) 85, 202, 273, 274, 378, 396
BmsI GCATC 3 cut(s) 637, 643, 679
BpiI GAAGAC 1 cut(s) 24
BpmI CTGGAG 1 cut(s) 360
Bpu10I CCTNAGC 1 cut(s) 531
BpuEI CTTGAG 2 cut(s) 215, 683
BpuMI CCSGG 3 cut(s) 85, 273, 274
BsaHI GRCGYC 2 cut(s) 80, 104
BsaJI CCNNGG 1 cut(s) 272
Bsc4I CCNNNNNNNGG 1 cut(s) 201
BseBI CCWGG 3 cut(s) 202, 378, 396
BseDI CCNNGG 1 cut(s) 272
BseGI GGATG 1 cut(s) 6
BseLI CCNNNNNNNGG 1 cut(s) 201
BseMII CTCAG 1 cut(s) 522
BseRI GAGGAG 1 cut(s) 757
BseX3I CGGCCG 1 cut(s) 68
BseXI GCAGC 3 cut(s) 195, 643, 778
BsgI GTGCAG 1 cut(s) 333
Bsh1236I CGCG 1 cut(s) 179
Bsh1285I CGRYCG 1 cut(s) 71
BshFI GGCC 3 cut(s) 5, 70, 563
BsiEI CGRYCG 1 cut(s) 71
BsiHKCI CYCGRG 2 cut(s) 272, 440
BsiSI CCGG 2 cut(s) 84, 273
BslFI GGGAC 1 cut(s) 525
BslI CCNNNNNNNGG 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 525
BsmI GAATGC 1 cut(s) 329
BsnI GGCC 3 cut(s) 5, 70, 563
BsoBI CYCGRG 2 cut(s) 272, 440
Bsp143I GATC 4 cut(s) 161, 238, 463, 595
BspACI CCGC 4 cut(s) 67, 93, 177, 683
BspANI GGCC 3 cut(s) 5, 70, 563
BspCNI CTCAG 1 cut(s) 523
BspFNI CGCG 1 cut(s) 179
BspHI TCATGA 1 cut(s) 492
BspLI GGNNCC 3 cut(s) 382, 438, 513
BspMI ACCTGC 2 cut(s) 271, 688
BspPI GGATC 4 cut(s) 169, 233, 471, 590
BssECI CCNNGG 1 cut(s) 272
BssMI GATC 4 cut(s) 161, 238, 463, 595
BssNI GRCGYC 2 cut(s) 80, 104
Bst2UI CCWGG 3 cut(s) 202, 378, 396
Bst4CI ACNGT 1 cut(s) 418
BstACI GRCGYC 2 cut(s) 80, 104
BstC8I GCNNGC 2 cut(s) 670, 674
BstDEI CTNAG 2 cut(s) 389, 531
BstF5I GGATG 1 cut(s) 6
BstFNI CGCG 1 cut(s) 179
BstHHI GCGC 1 cut(s) 661
BstKTI GATC 4 cut(s) 164, 241, 466, 598
BstMBI GATC 4 cut(s) 161, 238, 463, 595
BstMCI CGRYCG 1 cut(s) 71
BstMWI GCNNNNNNNGC 4 cut(s) 553, 656, 665, 689
BstNI CCWGG 3 cut(s) 202, 378, 396
BstSCI CCNGG 6 cut(s) 83, 200, 271, 272, 376, 394
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 3 cut(s) 195, 643, 778
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
BstZI CGGCCG 1 cut(s) 68
BsuRI GGCC 3 cut(s) 5, 70, 563
BtsCI GGATG 1 cut(s) 6
BveI ACCTGC 2 cut(s) 271, 688
Cac8I GCNNGC 2 cut(s) 670, 674
CciI TCATGA 1 cut(s) 492
CfoI GCGC 1 cut(s) 661
Cfr13I GGNCC 2 cut(s) 512, 695
Cfr9I CCCGGG 1 cut(s) 272
CseI GACGC 2 cut(s) 359, 623
Csp6I GTAC 1 cut(s) 527
CviAII CATG 3 cut(s) 367, 493, 792
CviQI GTAC 1 cut(s) 527
DdeI CTNAG 2 cut(s) 389, 531
DpnI GATC 4 cut(s) 163, 240, 465, 597
DpnII GATC 4 cut(s) 161, 238, 463, 595
DraI TTTAAA 1 cut(s) 487
DrdI GACNNNNNNGTC 1 cut(s) 47
DriI GACNNNNNGTC 1 cut(s) 19
DseDI GACNNNNNNGTC 1 cut(s) 47
EaeI YGGCCR 3 cut(s) 3, 68, 561
EagI CGGCCG 1 cut(s) 68
Eam1105I GACNNNNNGTC 1 cut(s) 19
EclXI CGGCCG 1 cut(s) 68
Eco32I GATATC 1 cut(s) 755
Eco47I GGWCC 2 cut(s) 512, 695
Eco52I CGGCCG 1 cut(s) 68
Eco57I CTGAAG 1 cut(s) 650
Eco88I CYCGRG 2 cut(s) 272, 440
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 3 cut(s) 200, 376, 394
EcoRV GATATC 1 cut(s) 755
FaeI CATG 3 cut(s) 370, 496, 795
FaiI YATR 8 cut(s) 141, 368, 410, 494, 525, 620, 725, 793
FaqI GGGAC 1 cut(s) 525
FatI CATG 3 cut(s) 366, 492, 791
Fnu4HI GCNGC 6 cut(s) 68, 93, 177, 209, 657, 767
Fsp4HI GCNGC 6 cut(s) 68, 93, 177, 209, 657, 767
FspBI CTAG 2 cut(s) 458, 593
GlaI GCGC 1 cut(s) 660
GluI GCNGC 6 cut(s) 68, 93, 177, 209, 657, 767
GsuI CTGGAG 1 cut(s) 360
HaeIII GGCC 3 cut(s) 5, 70, 563
HapII CCGG 2 cut(s) 84, 273
HgaI GACGC 2 cut(s) 359, 623
HhaI GCGC 1 cut(s) 661
Hin1I GRCGYC 2 cut(s) 80, 104
Hin1II CATG 3 cut(s) 370, 496, 795
Hin6I GCGC 1 cut(s) 659
HinP1I GCGC 1 cut(s) 659
HinfI GANTC 4 cut(s) 166, 217, 403, 785
HpaII CCGG 2 cut(s) 84, 273
HphI GGTGA 2 cut(s) 517, 785
Hpy166II GTNNAC 2 cut(s) 269, 784
Hpy188I TCNGA 4 cut(s) 40, 78, 216, 710
Hpy188III TCNNGA 4 cut(s) 440, 493, 593, 599
Hpy8I GTNNAC 2 cut(s) 269, 784
Hpy99I CGWCG 9 cut(s) 28, 31, 44, 106, 109, 118, 121, 161, 200
HpyAV CCTTC 5 cut(s) 496, 502, 707, 730, 770
HpyCH4III ACNGT 1 cut(s) 418
HpyCH4IV ACGT 2 cut(s) 80, 104
HpyCH4V TGCA 2 cut(s) 350, 791
HpyF10VI GCNNNNNNNGC 4 cut(s) 553, 656, 665, 689
HpyF3I CTNAG 2 cut(s) 389, 531
HpySE526I ACGT 2 cut(s) 80, 104
Hsp92I GRCGYC 2 cut(s) 80, 104
Hsp92II CATG 3 cut(s) 370, 496, 795
HspAI GCGC 1 cut(s) 659
Kzo9I GATC 4 cut(s) 161, 238, 463, 595
LmnI GCTCC 3 cut(s) 133, 380, 553
Lsp1109I GCAGC 3 cut(s) 195, 643, 778
LweI GCATC 3 cut(s) 637, 643, 679
MaeI CTAG 2 cut(s) 458, 593
MaeII ACGT 2 cut(s) 80, 104
MaeIII GTNAC 2 cut(s) 97, 143
MalI GATC 4 cut(s) 163, 240, 465, 597
MboI GATC 4 cut(s) 161, 238, 463, 595
MboII GAAGA 3 cut(s) 24, 52, 698
MflI RGATCY 1 cut(s) 595
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 181, 300
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 211, 412, 779
MmeI TCCRAC 2 cut(s) 63, 239
MnlI CCTC 6 cut(s) 121, 384, 427, 658, 735, 738
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 303, 480, 486
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 84, 273
MspR9I CCNGG 6 cut(s) 85, 202, 273, 274, 378, 396
Mva1269I GAATGC 1 cut(s) 329
MvaI CCWGG 3 cut(s) 202, 378, 396
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 4 cut(s) 553, 656, 665, 689
NciI CCSGG 3 cut(s) 85, 273, 274
NdeII GATC 4 cut(s) 161, 238, 463, 595
NlaIII CATG 3 cut(s) 370, 496, 795
NlaIV GGNNCC 3 cut(s) 382, 438, 513
NmeAIII GCCGAG 1 cut(s) 742
PagI TCATGA 1 cut(s) 492
PcsI WCGNNNNNNNCGW 5 cut(s) 32, 110, 113, 153, 212
PctI GAATGC 1 cut(s) 329
PfeI GAWTC 1 cut(s) 166
PflFI GACNNNGTC 1 cut(s) 106
PfoI TCCNGGA 2 cut(s) 83, 376
PkrI GCNGC 6 cut(s) 69, 94, 178, 210, 658, 768
PleI GAGTC 3 cut(s) 211, 411, 779
PpsI GAGTC 3 cut(s) 211, 411, 779
Psp6I CCWGG 3 cut(s) 200, 376, 394
PspGI CCWGG 3 cut(s) 200, 376, 394
PspN4I GGNNCC 3 cut(s) 382, 438, 513
PspPI GGNCC 2 cut(s) 512, 695
PsuI RGATCY 1 cut(s) 595
PsyI GACNNNGTC 1 cut(s) 106
RsaI GTAC 1 cut(s) 528
RsaNI GTAC 1 cut(s) 527
SaqAI TTAA 3 cut(s) 303, 480, 486
SatI GCNGC 6 cut(s) 68, 93, 177, 209, 657, 767
Sau3AI GATC 4 cut(s) 161, 238, 463, 595
Sau96I GGNCC 2 cut(s) 512, 695
SchI GAGTC 3 cut(s) 211, 412, 779
ScrFI CCNGG 6 cut(s) 85, 202, 273, 274, 378, 396
SfaNI GCATC 3 cut(s) 637, 643, 679
SinI GGWCC 2 cut(s) 512, 695
SmaI CCCGGG 1 cut(s) 274
SmlI CTYRAG 2 cut(s) 230, 662
SmoI CTYRAG 2 cut(s) 230, 662
SpeI ACTAGT 1 cut(s) 457
Sse9I AATT 2 cut(s) 181, 300
SsiI CCGC 4 cut(s) 67, 93, 177, 683
SspMI CTAG 2 cut(s) 458, 593
StyD4I CCNGG 6 cut(s) 83, 200, 271, 272, 376, 394
TaaI ACNGT 1 cut(s) 418
TaiI ACGT 2 cut(s) 83, 107
TaqI TCGA 2 cut(s) 164, 237
TasI AATT 2 cut(s) 181, 300
TauI GCSGC 3 cut(s) 70, 95, 179
TfiI GAWTC 1 cut(s) 166
Tru1I TTAA 3 cut(s) 303, 480, 486
Tru9I TTAA 3 cut(s) 303, 480, 486
TseI GCWGC 3 cut(s) 208, 656, 766
TspDTI ATGAA 3 cut(s) 355, 465, 744
TspGWI ACGGA 3 cut(s) 57, 174, 184
TspMI CCCGGG 1 cut(s) 272
Tth111I GACNNNGTC 1 cut(s) 106
VpaK11BI GGWCC 2 cut(s) 512, 695
XapI RAATTY 1 cut(s) 181
XbaI TCTAGA 1 cut(s) 592
XmaI CCCGGG 1 cut(s) 272
XspI CTAG 2 cut(s) 458, 593
ZraI GACGTC 2 cut(s) 81, 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.