MD10G1304100.v1.1

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007622
Physical Location & Seq
Reverse (-)
39040114 .. 39042653
2540 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1304100.v1.1.491

Sequence Viewer

Length: 801 bp
ATGGCCATCGCTGACCAAAAGTCGTCGTCGTCGTCTTCGTCTTCGTCCGACGGAAAAGTCTGGGGCTTCTTCAAGCGACCGTTTCGGACGTCGAGGAACGCCGCCGTTACGACGTCGTCCTCGTCGTCGCACAACGCTCCCCACCACCATAGTAACGCCCAGGGCGACGGATCGAATCAAAACGCCGCGAATTCGGTGTCGTCCGTCGCCAGGTCGCTGCTTCCGACTCGCCGTAGACTCAAGCTCGATCCCAACAATAAACTCTATTTTCCCTGTGAACCCGGAAAGCAGGTTAGGAGTGCCATTAGAATTAAAAACACCAGCAAGTCCTTTGTAGCATTCAAGTTCCAAACAACTGCACCGAAAAGTTGTTTCATGCGTCCTCCAGGAGCTATTCTTAGTCCAGGCGAGAGTCTTATAGCAACTGTATTCAAGTTTGTCGAGGCCCCCGAGAAGAATGAAAAACTAGTGGATCAAAAGAGCAGAGTTAAGTTTAAAATCATGAGCTTGAAGGTGAAGGGACCAATGGAATATGTACCTGAGCTGTTTGATGAGCAAAAGGACCAAGTGGCCGTAGAGCAGATACTACGGGTTGTCTTTCTAGATCCAGAGCGTTCTACTCCTGCTATGGAAAAACTGAAGCGCCAGTTGGCTGATGCCGATGCTGCGCTGGAGGCACGCAAGAAACCTGCGGAAGAAGCTGGTCCAAAGATTATTGGAGAAGGACTTGTCATAGATGAATGGGAGAGGAGGGAAAGATATCTCGCCCGGCAGCAAGTTGATGGCGTGGACTCCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

267

Amino Acids

29.5

Weight (kDa)

9.72

Isoelectric Point (pI)

49.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 80 - 190 2e-21 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 56
AatII GACGTC 2 cut(s) 92, 116
Acc36I ACCTGC 2 cut(s) 280, 697
AccI GTMKAC 1 cut(s) 235
AccII CGCG 1 cut(s) 188
AciI CCGC 3 cut(s) 102, 186, 692
AclWI GGATC 4 cut(s) 178, 242, 480, 599
AcoI YGGCCR 2 cut(s) 3, 570
AcsI RAATTY 1 cut(s) 190
AcuI CTGAAG 1 cut(s) 659
AcyI GRCGYC 2 cut(s) 89, 113
AfaI GTAC 1 cut(s) 537
AfiI CCNNNNNNNGG 1 cut(s) 210
AgsI TTSAA 4 cut(s) 73, 343, 433, 511
AhdI GACNNNNNGTC 1 cut(s) 19
AhlI ACTAGT 1 cut(s) 466
AjnI CCWGG 4 cut(s) 159, 209, 385, 403
AjuI GAANNNNNNNTTGG 2 cut(s) 632, 664
AluBI AGCT 5 cut(s) 244, 392, 507, 544, 701
AluI AGCT 5 cut(s) 244, 392, 507, 544, 701
AlwI GGATC 4 cut(s) 178, 242, 480, 599
Ama87I CYCGRG 1 cut(s) 449
AoxI GGCC 3 cut(s) 3, 444, 570
ApeKI GCWGC 3 cut(s) 217, 665, 772
ApoI RAATTY 1 cut(s) 190
ArsI GACNNNNNNTTYG 2 cut(s) 182, 214
AspLEI GCGC 2 cut(s) 645, 670
AspS9I GGNCC 4 cut(s) 445, 521, 562, 704
AsuC2I CCSGG 2 cut(s) 282, 769
AsuHPI GGTGA 1 cut(s) 526
AvaI CYCGRG 1 cut(s) 449
AvaII GGWCC 3 cut(s) 521, 562, 704
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 2 cut(s) 27, 33
BbvI GCAGC 3 cut(s) 204, 652, 784
BccI CCATC 2 cut(s) 14, 776
BceAI ACGGC 3 cut(s) 89, 216, 557
BciT130I CCWGG 4 cut(s) 161, 211, 387, 405
BcnI CCSGG 2 cut(s) 282, 769
BcuI ACTAGT 1 cut(s) 466
BfaI CTAG 2 cut(s) 467, 602
BfoI RGCGCY 1 cut(s) 646
BfuAI ACCTGC 2 cut(s) 280, 697
BisI GCNGC 5 cut(s) 102, 186, 218, 666, 773
BlsI GCNGC 5 cut(s) 103, 187, 219, 667, 774
Bme1390I CCNGG 6 cut(s) 161, 211, 282, 387, 405, 769
Bme18I GGWCC 3 cut(s) 521, 562, 704
BmeRI GACNNNNNGTC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 449
BmgT120I GGNCC 4 cut(s) 445, 521, 562, 704
BmiI GGNNCC 2 cut(s) 447, 522
BmrFI CCNGG 6 cut(s) 161, 211, 282, 387, 405, 769
BmsI GCATC 2 cut(s) 646, 652
BpiI GAAGAC 2 cut(s) 27, 33
BpmI CTGGAG 2 cut(s) 369, 692
Bpu10I CCTNAGC 1 cut(s) 540
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 2 cut(s) 282, 769
BsaHI GRCGYC 2 cut(s) 89, 113
BsaJI CCNNGG 2 cut(s) 159, 160
Bsc4I CCNNNNNNNGG 1 cut(s) 210
Bse1I ACTGG 1 cut(s) 646
BseBI CCWGG 4 cut(s) 161, 211, 387, 405
BseDI CCNNGG 2 cut(s) 159, 160
BseLI CCNNNNNNNGG 1 cut(s) 210
BseMII CTCAG 1 cut(s) 531
BseNI ACTGG 1 cut(s) 646
BseRI GAGGAG 1 cut(s) 763
BseXI GCAGC 3 cut(s) 204, 652, 784
BsgI GTGCAG 1 cut(s) 342
Bsh1236I CGCG 1 cut(s) 188
Bsh1285I CGRYCG 1 cut(s) 80
BshFI GGCC 3 cut(s) 5, 446, 572
BsiEI CGRYCG 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 449
BsiSI CCGG 2 cut(s) 282, 769
BslFI GGGAC 1 cut(s) 534
BslI CCNNNNNNNGG 1 cut(s) 210
BsmFI GGGAC 1 cut(s) 534
BsmI GAATGC 1 cut(s) 338
BsnI GGCC 3 cut(s) 5, 446, 572
BsoBI CYCGRG 1 cut(s) 449
Bsp143I GATC 4 cut(s) 170, 247, 472, 604
BspACI CCGC 3 cut(s) 102, 186, 692
BspANI GGCC 3 cut(s) 5, 446, 572
BspCNI CTCAG 1 cut(s) 532
BspFNI CGCG 1 cut(s) 188
BspHI TCATGA 1 cut(s) 501
BspLI GGNNCC 2 cut(s) 447, 522
BspMI ACCTGC 2 cut(s) 280, 697
BspPI GGATC 4 cut(s) 178, 242, 480, 599
BsrI ACTGG 1 cut(s) 646
BssECI CCNNGG 2 cut(s) 159, 160
BssMI GATC 4 cut(s) 170, 247, 472, 604
BssNI GRCGYC 2 cut(s) 89, 113
Bst2UI CCWGG 4 cut(s) 161, 211, 387, 405
Bst4CI ACNGT 2 cut(s) 81, 427
BstACI GRCGYC 2 cut(s) 89, 113
BstC8I GCNNGC 1 cut(s) 679
BstDEI CTNAG 2 cut(s) 398, 540
BstFNI CGCG 1 cut(s) 188
BstH2I RGCGCY 1 cut(s) 646
BstHHI GCGC 2 cut(s) 645, 670
BstKTI GATC 4 cut(s) 173, 250, 475, 607
BstMBI GATC 4 cut(s) 170, 247, 472, 604
BstMCI CGRYCG 1 cut(s) 80
BstMWI GCNNNNNNNGC 3 cut(s) 665, 674, 698
BstNI CCWGG 4 cut(s) 161, 211, 387, 405
BstSCI CCNGG 6 cut(s) 159, 209, 280, 385, 403, 767
BstUI CGCG 1 cut(s) 188
BstV1I GCAGC 3 cut(s) 204, 652, 784
BstV2I GAAGAC 2 cut(s) 27, 33
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BsuRI GGCC 3 cut(s) 5, 446, 572
BveI ACCTGC 2 cut(s) 280, 697
Cac8I GCNNGC 1 cut(s) 679
CciI TCATGA 1 cut(s) 501
CfoI GCGC 2 cut(s) 645, 670
Cfr13I GGNCC 4 cut(s) 445, 521, 562, 704
CseI GACGC 1 cut(s) 368
Csp6I GTAC 1 cut(s) 536
CviAII CATG 3 cut(s) 376, 502, 798
CviQI GTAC 1 cut(s) 536
DdeI CTNAG 2 cut(s) 398, 540
DpnI GATC 4 cut(s) 172, 249, 474, 606
DpnII GATC 4 cut(s) 170, 247, 472, 604
DraI TTTAAA 1 cut(s) 496
DrdI GACNNNNNNGTC 1 cut(s) 56
DriI GACNNNNNGTC 1 cut(s) 19
DseDI GACNNNNNNGTC 1 cut(s) 56
EaeI YGGCCR 2 cut(s) 3, 570
Eam1105I GACNNNNNGTC 1 cut(s) 19
Eco32I GATATC 1 cut(s) 761
Eco47I GGWCC 3 cut(s) 521, 562, 704
Eco57I CTGAAG 1 cut(s) 659
Eco88I CYCGRG 1 cut(s) 449
EcoO109I RGGNCCY 1 cut(s) 445
EcoRI GAATTC 1 cut(s) 190
EcoRII CCWGG 4 cut(s) 159, 209, 385, 403
EcoRV GATATC 1 cut(s) 761
FaeI CATG 3 cut(s) 379, 505, 801
FaiI YATR 8 cut(s) 150, 377, 419, 503, 534, 629, 734, 799
FaqI GGGAC 1 cut(s) 534
FatI CATG 3 cut(s) 375, 501, 797
FblI GTMKAC 1 cut(s) 235
Fnu4HI GCNGC 5 cut(s) 102, 186, 218, 666, 773
Fsp4HI GCNGC 5 cut(s) 102, 186, 218, 666, 773
FspBI CTAG 2 cut(s) 467, 602
GlaI GCGC 2 cut(s) 644, 669
GluI GCNGC 5 cut(s) 102, 186, 218, 666, 773
GsuI CTGGAG 2 cut(s) 369, 692
HaeII RGCGCY 1 cut(s) 646
HaeIII GGCC 3 cut(s) 5, 446, 572
HapII CCGG 2 cut(s) 282, 769
HgaI GACGC 1 cut(s) 368
HhaI GCGC 2 cut(s) 645, 670
Hin1I GRCGYC 2 cut(s) 89, 113
Hin1II CATG 3 cut(s) 379, 505, 801
Hin6I GCGC 2 cut(s) 643, 668
HinP1I GCGC 2 cut(s) 643, 668
HinfI GANTC 5 cut(s) 175, 226, 237, 412, 791
HpaII CCGG 2 cut(s) 282, 769
HphI GGTGA 1 cut(s) 526
Hpy166II GTNNAC 3 cut(s) 236, 278, 790
Hpy188I TCNGA 3 cut(s) 49, 87, 225
Hpy188III TCNNGA 3 cut(s) 502, 602, 608
Hpy8I GTNNAC 3 cut(s) 236, 278, 790
HpyAV CCTTC 3 cut(s) 505, 511, 716
HpyCH4III ACNGT 2 cut(s) 81, 427
HpyCH4IV ACGT 2 cut(s) 89, 113
HpyCH4V TGCA 1 cut(s) 359
HpyF10VI GCNNNNNNNGC 3 cut(s) 665, 674, 698
HpyF3I CTNAG 2 cut(s) 398, 540
HpySE526I ACGT 2 cut(s) 89, 113
Hsp92I GRCGYC 2 cut(s) 89, 113
Hsp92II CATG 3 cut(s) 379, 505, 801
HspAI GCGC 2 cut(s) 643, 668
Kzo9I GATC 4 cut(s) 170, 247, 472, 604
LmnI GCTCC 2 cut(s) 142, 389
Lsp1109I GCAGC 3 cut(s) 204, 652, 784
LweI GCATC 2 cut(s) 646, 652
MaeI CTAG 2 cut(s) 467, 602
MaeII ACGT 2 cut(s) 89, 113
MaeIII GTNAC 2 cut(s) 106, 152
MalI GATC 4 cut(s) 172, 249, 474, 606
MboI GATC 4 cut(s) 170, 247, 472, 604
MboII GAAGA 5 cut(s) 27, 33, 61, 466, 707
MflI RGATCY 1 cut(s) 604
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 190, 309
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 220, 231, 421, 785
MmeI TCCRAC 2 cut(s) 72, 248
MnlI CCTC 7 cut(s) 87, 130, 393, 436, 667, 741, 744
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 312, 489, 495
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 282, 769
MspR9I CCNGG 6 cut(s) 161, 211, 282, 387, 405, 769
Mva1269I GAATGC 1 cut(s) 338
MvaI CCWGG 4 cut(s) 161, 211, 387, 405
MvnI CGCG 1 cut(s) 188
MwoI GCNNNNNNNGC 3 cut(s) 665, 674, 698
NciI CCSGG 2 cut(s) 282, 769
NdeII GATC 4 cut(s) 170, 247, 472, 604
NlaIII CATG 3 cut(s) 379, 505, 801
NlaIV GGNNCC 2 cut(s) 447, 522
PagI TCATGA 1 cut(s) 501
PasI CCCWGGG 1 cut(s) 160
PcsI WCGNNNNNNNCGW 7 cut(s) 29, 35, 119, 122, 162, 221, 447
PctI GAATGC 1 cut(s) 338
PfeI GAWTC 1 cut(s) 175
PflFI GACNNNGTC 1 cut(s) 115
PfoI TCCNGGA 1 cut(s) 385
PkrI GCNGC 5 cut(s) 103, 187, 219, 667, 774
PleI GAGTC 4 cut(s) 220, 231, 420, 785
PpsI GAGTC 4 cut(s) 220, 231, 420, 785
Psp6I CCWGG 4 cut(s) 159, 209, 385, 403
PspGI CCWGG 4 cut(s) 159, 209, 385, 403
PspN4I GGNNCC 2 cut(s) 447, 522
PspPI GGNCC 4 cut(s) 445, 521, 562, 704
PsuI RGATCY 1 cut(s) 604
PsyI GACNNNGTC 1 cut(s) 115
RsaI GTAC 1 cut(s) 537
RsaNI GTAC 1 cut(s) 536
SaqAI TTAA 3 cut(s) 312, 489, 495
SatI GCNGC 5 cut(s) 102, 186, 218, 666, 773
Sau3AI GATC 4 cut(s) 170, 247, 472, 604
Sau96I GGNCC 4 cut(s) 445, 521, 562, 704
SchI GAGTC 4 cut(s) 220, 231, 421, 785
ScrFI CCNGG 6 cut(s) 161, 211, 282, 387, 405, 769
SfaNI GCATC 2 cut(s) 646, 652
SinI GGWCC 3 cut(s) 521, 562, 704
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
SpeI ACTAGT 1 cut(s) 466
Sse9I AATT 2 cut(s) 190, 309
SsiI CCGC 3 cut(s) 102, 186, 692
SspMI CTAG 2 cut(s) 467, 602
StyD4I CCNGG 6 cut(s) 159, 209, 280, 385, 403, 767
TaaI ACNGT 2 cut(s) 81, 427
TaiI ACGT 2 cut(s) 92, 116
TaqI TCGA 4 cut(s) 92, 173, 246, 441
TasI AATT 2 cut(s) 190, 309
TauI GCSGC 2 cut(s) 104, 188
TfiI GAWTC 1 cut(s) 175
Tru1I TTAA 3 cut(s) 312, 489, 495
Tru9I TTAA 3 cut(s) 312, 489, 495
TseI GCWGC 3 cut(s) 217, 665, 772
TspDTI ATGAA 3 cut(s) 364, 474, 753
TspGWI ACGGA 3 cut(s) 66, 183, 193
Tth111I GACNNNGTC 1 cut(s) 115
VpaK11BI GGWCC 3 cut(s) 521, 562, 704
XapI RAATTY 1 cut(s) 190
XbaI TCTAGA 1 cut(s) 601
XmiI GTMKAC 1 cut(s) 235
XspI CTAG 2 cut(s) 467, 602
ZraI GACGTC 2 cut(s) 90, 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.