Rh5DG046000

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007622
Physical Location & Seq
Forward (+)
3809367 .. 3812822
3456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG046000.1

Sequence Viewer

Length: 600 bp
ATGGCCATAGCTGACCACAAGTCTTCGTCGTCTTCGTCTGACGGAAAAGTCTGGGGCTTCTTCAAACGGCCGTTTCGACCGTCCGGTAACGCCGCCGGCACTACGACGTCTTCTTCTTCTATGTCGCACCACCAGCCGAACGGCGACGGATCGATTCCTCACGCCTCGAGTTCCGTGTCGTCGGTAGCCAGGTCGCTGCTTCCGACTCGTCGTAGACTGAAGCTCGATCCCTGCAATAAGCTATATTTTCCTTGTGAACCTGGGAAGCAGGTTAGGAGTGCCATTAGAATTAAAAACACAAGCAAGTCCCATGTAGCGTTCAAGTTTCAAACGACTGCACCCAAAAGTTGTTTCATGCGTCCTCCTGGTGCTATTCTTAGTCCTGGTGAGAGTCTCATAGCCACAGTGTTCAAGTTTGTGGAGGTTCCTGAGAACAATGAAAAACTAGTGGATCAAAAGAGCCGAGTTAAATTTAAAATAATGAGCCTGAAGGTGAAGGGGCCAATGGACTATGTACCTGAGCTGGTGTGTTACATGCATCCTTCAAGTGCTATCTTTAATTATGGTGTTACTTTGTTAATCATGTTCCTAAGTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

199

Amino Acids

21.84

Weight (kDa)

9.98

Isoelectric Point (pI)

53.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 73 - 173 1.4e-21 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 47
AatII GACGTC 1 cut(s) 110
Acc36I ACCTGC 1 cut(s) 259
AccI GTMKAC 1 cut(s) 214
AciI CCGC 1 cut(s) 93
AclWI GGATC 3 cut(s) 157, 221, 459
AcoI YGGCCR 2 cut(s) 3, 68
AcsI RAATTY 1 cut(s) 470
AcuI CTGAAG 2 cut(s) 239, 509
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 1 cut(s) 516
AgsI TTSAA 5 cut(s) 64, 322, 329, 412, 546
AhdI GACNNNNNGTC 1 cut(s) 19
AhlI ACTAGT 1 cut(s) 445
AjnI CCWGG 4 cut(s) 188, 259, 364, 382
AjuI GAANNNNNNNTTGG 2 cut(s) 335, 367
AluBI AGCT 4 cut(s) 11, 223, 241, 523
AluI AGCT 4 cut(s) 11, 223, 241, 523
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 3 cut(s) 157, 221, 459
Ama87I CYCGRG 1 cut(s) 166
AoxI GGCC 3 cut(s) 3, 68, 500
ApeKI GCWGC 1 cut(s) 196
ApoI RAATTY 1 cut(s) 470
AspS9I GGNCC 1 cut(s) 500
AsuHPI GGTGA 2 cut(s) 398, 505
AvaI CYCGRG 1 cut(s) 166
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 3 cut(s) 15, 24, 102
BbvI GCAGC 1 cut(s) 183
BceAI ACGGC 3 cut(s) 55, 83, 157
BciT130I CCWGG 4 cut(s) 190, 261, 366, 384
BcoDI GTCTC 1 cut(s) 398
BcuI ACTAGT 1 cut(s) 445
BfaI CTAG 1 cut(s) 446
BfuAI ACCTGC 1 cut(s) 259
BisI GCNGC 2 cut(s) 93, 197
BlsI GCNGC 2 cut(s) 94, 198
Bme1390I CCNGG 4 cut(s) 190, 261, 366, 384
BmeRI GACNNNNNGTC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 500
BmiI GGNNCC 2 cut(s) 426, 501
BmrFI CCNGG 4 cut(s) 190, 261, 366, 384
BmsI GCATC 1 cut(s) 547
BpiI GAAGAC 3 cut(s) 15, 24, 102
Bpu10I CCTNAGC 1 cut(s) 519
Bsa29I ATCGAT 1 cut(s) 152
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 260
BsaWI WCCGGW 1 cut(s) 83
Bse118I RCCGGY 1 cut(s) 95
BseBI CCWGG 4 cut(s) 190, 261, 366, 384
BseCI ATCGAT 1 cut(s) 152
BseDI CCNNGG 1 cut(s) 260
BseGI GGATG 1 cut(s) 538
BseMII CTCAG 2 cut(s) 420, 510
BseX3I CGGCCG 1 cut(s) 68
BseXI GCAGC 1 cut(s) 183
BsgI GTGCAG 1 cut(s) 321
Bsh1285I CGRYCG 2 cut(s) 71, 80
BshFI GGCC 3 cut(s) 5, 70, 502
BshVI ATCGAT 1 cut(s) 152
BsiEI CGRYCG 2 cut(s) 71, 80
BsiHKCI CYCGRG 1 cut(s) 166
BsiSI CCGG 2 cut(s) 84, 96
BslFI GGGAC 1 cut(s) 292
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 292
BsnI GGCC 3 cut(s) 5, 70, 502
BsoBI CYCGRG 1 cut(s) 166
Bsp143I GATC 3 cut(s) 149, 226, 451
BspACI CCGC 1 cut(s) 93
BspANI GGCC 3 cut(s) 5, 70, 502
BspCNI CTCAG 2 cut(s) 421, 511
BspDI ATCGAT 1 cut(s) 152
BspLI GGNNCC 2 cut(s) 426, 501
BspMI ACCTGC 1 cut(s) 259
BspPI GGATC 3 cut(s) 157, 221, 459
BsrFI RCCGGY 1 cut(s) 95
BssAI RCCGGY 1 cut(s) 95
BssECI CCNNGG 1 cut(s) 260
BssMI GATC 3 cut(s) 149, 226, 451
BssNI GRCGYC 1 cut(s) 107
Bst2UI CCWGG 4 cut(s) 190, 261, 366, 384
Bst4CI ACNGT 2 cut(s) 81, 406
BstACI GRCGYC 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 97
BstDEI CTNAG 4 cut(s) 377, 429, 519, 590
BstF5I GGATG 1 cut(s) 538
BstKTI GATC 3 cut(s) 152, 229, 454
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 3 cut(s) 149, 226, 451
BstMCI CGRYCG 2 cut(s) 71, 80
BstMWI GCNNNNNNNGC 1 cut(s) 133
BstNI CCWGG 4 cut(s) 190, 261, 366, 384
BstNSI RCATGY 1 cut(s) 538
BstSCI CCNGG 4 cut(s) 188, 259, 364, 382
BstV1I GCAGC 1 cut(s) 183
BstV2I GAAGAC 3 cut(s) 15, 24, 102
BstZI CGGCCG 1 cut(s) 68
Bsu15I ATCGAT 1 cut(s) 152
BsuRI GGCC 3 cut(s) 5, 70, 502
BsuTUI ATCGAT 1 cut(s) 152
BtsCI GGATG 1 cut(s) 538
BtsIMutI CAGTG 1 cut(s) 411
BveI ACCTGC 1 cut(s) 259
Cac8I GCNNGC 1 cut(s) 97
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 500
ClaI ATCGAT 1 cut(s) 152
CseI GACGC 1 cut(s) 347
Csp6I GTAC 1 cut(s) 515
CviAII CATG 4 cut(s) 311, 355, 535, 583
CviQI GTAC 1 cut(s) 515
DdeI CTNAG 4 cut(s) 377, 429, 519, 590
DpnI GATC 3 cut(s) 151, 228, 453
DpnII GATC 3 cut(s) 149, 226, 451
DraI TTTAAA 1 cut(s) 475
DrdI GACNNNNNNGTC 1 cut(s) 47
DriI GACNNNNNGTC 1 cut(s) 19
DseDI GACNNNNNNGTC 1 cut(s) 47
EaeI YGGCCR 2 cut(s) 3, 68
EagI CGGCCG 1 cut(s) 68
Eam1105I GACNNNNNGTC 1 cut(s) 19
EclXI CGGCCG 1 cut(s) 68
Eco52I CGGCCG 1 cut(s) 68
Eco57I CTGAAG 2 cut(s) 239, 509
Eco88I CYCGRG 1 cut(s) 166
EcoRII CCWGG 4 cut(s) 188, 259, 364, 382
EcoT22I ATGCAT 1 cut(s) 540
FaeI CATG 4 cut(s) 314, 358, 538, 586
FaqI GGGAC 1 cut(s) 292
FatI CATG 4 cut(s) 310, 354, 534, 582
FblI GTMKAC 1 cut(s) 214
Fnu4HI GCNGC 2 cut(s) 93, 197
FokI GGATG 1 cut(s) 525
Fsp4HI GCNGC 2 cut(s) 93, 197
FspBI CTAG 1 cut(s) 446
GluI GCNGC 2 cut(s) 93, 197
HaeIII GGCC 3 cut(s) 5, 70, 502
HapII CCGG 2 cut(s) 84, 96
HgaI GACGC 1 cut(s) 347
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 4 cut(s) 314, 358, 538, 586
HinfI GANTC 3 cut(s) 154, 205, 391
HpaII CCGG 2 cut(s) 84, 96
HphI GGTGA 2 cut(s) 398, 505
Hpy166II GTNNAC 2 cut(s) 215, 257
Hpy188I TCNGA 2 cut(s) 40, 204
Hpy188III TCNNGA 1 cut(s) 428
Hpy8I GTNNAC 2 cut(s) 215, 257
Hpy99I CGWCG 5 cut(s) 31, 109, 149, 184, 213
HpyAV CCTTC 3 cut(s) 484, 490, 552
HpyCH4III ACNGT 2 cut(s) 81, 406
HpyCH4IV ACGT 1 cut(s) 107
HpyCH4V TGCA 3 cut(s) 234, 338, 538
HpyF10VI GCNNNNNNNGC 1 cut(s) 133
HpyF3I CTNAG 4 cut(s) 377, 429, 519, 590
HpySE526I ACGT 1 cut(s) 107
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 4 cut(s) 314, 358, 538, 586
KroI GCCGGC 1 cut(s) 95
KroNI GCCGGC 1 cut(s) 97
Kzo9I GATC 3 cut(s) 149, 226, 451
Lsp1109I GCAGC 1 cut(s) 183
LweI GCATC 1 cut(s) 547
MaeI CTAG 1 cut(s) 446
MaeII ACGT 1 cut(s) 107
MaeIII GTNAC 3 cut(s) 86, 530, 568
MalI GATC 3 cut(s) 151, 228, 453
MboI GATC 3 cut(s) 149, 226, 451
MboII GAAGA 6 cut(s) 15, 24, 52, 102, 105, 108
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 288, 470, 559
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 199, 400
MmeI TCCRAC 1 cut(s) 227
MnlI CCTC 4 cut(s) 168, 175, 372, 415
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 540
MroNI GCCGGC 1 cut(s) 95
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 291, 468, 474, 558, 578
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 84, 96
MspR9I CCNGG 4 cut(s) 190, 261, 366, 384
MvaI CCWGG 4 cut(s) 190, 261, 366, 384
MwoI GCNNNNNNNGC 1 cut(s) 133
NaeI GCCGGC 1 cut(s) 97
NdeII GATC 3 cut(s) 149, 226, 451
NgoMIV GCCGGC 1 cut(s) 95
NlaIII CATG 4 cut(s) 314, 358, 538, 586
NlaIV GGNNCC 2 cut(s) 426, 501
NmeAIII GCCGAG 1 cut(s) 488
NsiI ATGCAT 1 cut(s) 540
NspI RCATGY 1 cut(s) 538
PaeR7I CTCGAG 1 cut(s) 166
PcsI WCGNNNNNNNCGW 3 cut(s) 32, 73, 200
PdiI GCCGGC 1 cut(s) 97
PfeI GAWTC 1 cut(s) 154
PkrI GCNGC 2 cut(s) 94, 198
PleI GAGTC 2 cut(s) 199, 399
PpsI GAGTC 2 cut(s) 199, 399
Psp6I CCWGG 4 cut(s) 188, 259, 364, 382
PspGI CCWGG 4 cut(s) 188, 259, 364, 382
PspN4I GGNNCC 2 cut(s) 426, 501
PspPI GGNCC 1 cut(s) 500
PspXI VCTCGAGB 1 cut(s) 166
RsaI GTAC 1 cut(s) 516
RsaNI GTAC 1 cut(s) 515
SaqAI TTAA 5 cut(s) 291, 468, 474, 558, 578
SatI GCNGC 2 cut(s) 93, 197
Sau3AI GATC 3 cut(s) 149, 226, 451
Sau96I GGNCC 1 cut(s) 500
SchI GAGTC 2 cut(s) 199, 400
ScrFI CCNGG 4 cut(s) 190, 261, 366, 384
SfaNI GCATC 1 cut(s) 547
Sfr274I CTCGAG 1 cut(s) 166
SlaI CTCGAG 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
SpeI ACTAGT 1 cut(s) 445
Sse9I AATT 3 cut(s) 288, 470, 559
SsiI CCGC 1 cut(s) 93
SspMI CTAG 1 cut(s) 446
StyD4I CCNGG 4 cut(s) 188, 259, 364, 382
TaaI ACNGT 2 cut(s) 81, 406
TaiI ACGT 1 cut(s) 110
TaqI TCGA 4 cut(s) 76, 152, 167, 225
TasI AATT 3 cut(s) 288, 470, 559
TauI GCSGC 1 cut(s) 95
TfiI GAWTC 1 cut(s) 154
Tru1I TTAA 5 cut(s) 291, 468, 474, 558, 578
Tru9I TTAA 5 cut(s) 291, 468, 474, 558, 578
TscAI CASTG 1 cut(s) 411
TseI GCWGC 1 cut(s) 196
TspDTI ATGAA 2 cut(s) 343, 453
TspGWI ACGGA 3 cut(s) 57, 162, 163
TspRI CASTG 1 cut(s) 411
XapI RAATTY 1 cut(s) 470
XceI RCATGY 1 cut(s) 538
XhoI CTCGAG 1 cut(s) 166
XmiI GTMKAC 1 cut(s) 214
XspI CTAG 1 cut(s) 446
ZraI GACGTC 1 cut(s) 108
Zsp2I ATGCAT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.