FvH4_3g03880

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007622
Physical Location & Seq
Forward (+)
2158074 .. 2161938
3865 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03880.t1

Sequence Viewer

Length: 786 bp
ATGCCCATAGCTGACCACAAGTCGTCGACTTCGTCTGACAGAAAAGTCTGGGGCTTCTTCAAACGGCCGTTTCGACCGTCCGGTAACGCCGCCGCCACTACGACGTCGTCTTCTTCCATGTCGCACGGCCACCCCCACCCCAACGGCGACGGATCGGTTCCTCAAGCCTCGAGCTCCGTGTCGTCCGTCGCCAGGTCGCTGCTTCCGACTCGTCGTAGACTGAAGCTCGATCCCTGCAATAAGCTCTACTTTCCTTGTGAGCCTGGGAAGCAGGTTCGGAGTGCCATTAGAATTAAAAACACAAGCAAGTCCCATGTAGCGTTCAAGTTTCAAACGACTGCACCAAAAAGTTGTTTCATGCGTCCTCCTGGTGCTATTCTTAATCCTGGCGAGAGTATCATAGCAACAGTGTTCAAGTTTGTGGAGGTTCCAGAGAACAATGAAAAATTAGTGGATCAAAAGAGCAGAGTTAAATTTAAAATAATGAGCCTAAAGGTGAAGGGGCCTATGGACTATGTACCTGAGCTGTTTGATGAGCAGAAGGAACAAGTAGCAATAGAGCAGATCTTACGGGTTGTCTTCCTGGATCCAGAGCGTTCCACCCCTGCCATGGAAAAATTGAAGCGTCAGTTGGCTGATGCTGATGCTGCGCTTGAGTCACGCAAGAAACCTCCAGAAGATACTGGACCAAAGATTATAGGAGAAGGTCTCGTGATAGATGAATGGAAGGAGCGGAGGGAAAGATATCTAGCTCGGCAGCAGGTTGAAGGGTTGGACTCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

262

Amino Acids

29.13

Weight (kDa)

9.66

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 74 - 183 6.9e-23 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 44
AatII GACGTC 1 cut(s) 107
Acc36I ACCTGC 2 cut(s) 262, 751
AccBSI CCGCTC 1 cut(s) 733
AccI GTMKAC 2 cut(s) 26, 217
AciI CCGC 3 cut(s) 90, 93, 733
AclWI GGATC 5 cut(s) 160, 224, 462, 581, 594
AcoI YGGCCR 2 cut(s) 65, 127
AcsI RAATTY 1 cut(s) 473
AcuI CTGAAG 1 cut(s) 242
AcyI GRCGYC 1 cut(s) 104
AfaI GTAC 1 cut(s) 519
AfiI CCNNNNNNNGG 2 cut(s) 192, 610
AgsI TTSAA 6 cut(s) 61, 325, 332, 415, 622, 767
AhdI GACNNNNNGTC 1 cut(s) 19
AjnI CCWGG 5 cut(s) 191, 262, 367, 385, 582
AjuI GAANNNNNNNTTGG 2 cut(s) 614, 646
AluBI AGCT 6 cut(s) 11, 174, 226, 244, 526, 752
AluI AGCT 6 cut(s) 11, 174, 226, 244, 526, 752
Alw21I GWGCWC 1 cut(s) 176
Alw26I GTCTC 1 cut(s) 713
AlwI GGATC 5 cut(s) 160, 224, 462, 581, 594
Ama87I CYCGRG 1 cut(s) 169
AoxI GGCC 3 cut(s) 65, 127, 503
ApeKI GCWGC 3 cut(s) 199, 647, 757
ApoI RAATTY 1 cut(s) 473
AspLEI GCGC 1 cut(s) 652
AspS9I GGNCC 2 cut(s) 503, 686
AsuHPI GGTGA 1 cut(s) 508
AvaI CYCGRG 1 cut(s) 169
AvaII GGWCC 1 cut(s) 686
BamHI GGATCC 1 cut(s) 586
BanII GRGCYC 1 cut(s) 176
BauI CACGAG 1 cut(s) 710
BbsI GAAGAC 2 cut(s) 102, 571
Bbv12I GWGCWC 1 cut(s) 176
BbvI GCAGC 3 cut(s) 186, 634, 769
BceAI ACGGC 4 cut(s) 52, 80, 142, 160
BciT130I CCWGG 5 cut(s) 193, 264, 369, 387, 584
BcoDI GTCTC 1 cut(s) 713
BfaI CTAG 1 cut(s) 749
BfuAI ACCTGC 2 cut(s) 262, 751
BglII AGATCT 1 cut(s) 564
BisI GCNGC 5 cut(s) 90, 93, 200, 648, 758
BlsI GCNGC 5 cut(s) 91, 94, 201, 649, 759
Bme1390I CCNGG 5 cut(s) 193, 264, 369, 387, 584
Bme18I GGWCC 1 cut(s) 686
BmeRI GACNNNNNGTC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 2 cut(s) 503, 686
BmiI GGNNCC 4 cut(s) 159, 429, 504, 588
BmrFI CCNGG 5 cut(s) 193, 264, 369, 387, 584
BmsI GCATC 2 cut(s) 628, 634
BpiI GAAGAC 2 cut(s) 102, 571
BplI GAGNNNNNCTC 2 cut(s) 693, 725
BpmI CTGGAG 1 cut(s) 657
Bpu10I CCTNAGC 1 cut(s) 522
BpuEI CTTGAG 2 cut(s) 147, 674
BsaHI GRCGYC 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 713
BsaJI CCNNGG 2 cut(s) 263, 609
BsaWI WCCGGW 1 cut(s) 80
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 610
Bse1I ACTGG 1 cut(s) 688
BseBI CCWGG 5 cut(s) 193, 264, 369, 387, 584
BseDI CCNNGG 2 cut(s) 263, 609
BseLI CCNNNNNNNGG 2 cut(s) 192, 610
BseMII CTCAG 1 cut(s) 513
BseNI ACTGG 1 cut(s) 688
BseX3I CGGCCG 1 cut(s) 65
BseXI GCAGC 3 cut(s) 186, 634, 769
BsgI GTGCAG 1 cut(s) 324
Bsh1285I CGRYCG 2 cut(s) 68, 77
BshFI GGCC 3 cut(s) 67, 129, 505
BsiEI CGRYCG 2 cut(s) 68, 77
BsiHKAI GWGCWC 1 cut(s) 176
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 1 cut(s) 81
BslFI GGGAC 1 cut(s) 295
BslI CCNNNNNNNGG 2 cut(s) 192, 610
BsmAI GTCTC 1 cut(s) 713
BsmFI GGGAC 1 cut(s) 295
BsnI GGCC 3 cut(s) 67, 129, 505
Bso31I GGTCTC 1 cut(s) 713
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 176
Bsp143I GATC 5 cut(s) 152, 229, 454, 564, 586
Bsp19I CCATGG 1 cut(s) 609
BspACI CCGC 3 cut(s) 90, 93, 733
BspANI GGCC 3 cut(s) 67, 129, 505
BspCNI CTCAG 1 cut(s) 514
BspLI GGNNCC 4 cut(s) 159, 429, 504, 588
BspMI ACCTGC 2 cut(s) 262, 751
BspPI GGATC 5 cut(s) 160, 224, 462, 581, 594
BspTNI GGTCTC 1 cut(s) 713
BsrBI CCGCTC 1 cut(s) 733
BsrI ACTGG 1 cut(s) 688
BssECI CCNNGG 2 cut(s) 263, 609
BssMI GATC 5 cut(s) 152, 229, 454, 564, 586
BssNI GRCGYC 1 cut(s) 104
BssSI CACGAG 1 cut(s) 710
BssT1I CCWWGG 1 cut(s) 609
Bst2BI CACGAG 1 cut(s) 710
Bst2UI CCWGG 5 cut(s) 193, 264, 369, 387, 584
Bst4CI ACNGT 2 cut(s) 78, 409
BstACI GRCGYC 1 cut(s) 104
BstDEI CTNAG 1 cut(s) 522
BstDSI CCRYGG 1 cut(s) 609
BstHHI GCGC 1 cut(s) 652
BstKTI GATC 5 cut(s) 155, 232, 457, 567, 589
BstMAI GTCTC 1 cut(s) 713
BstMBI GATC 5 cut(s) 152, 229, 454, 564, 586
BstMCI CGRYCG 2 cut(s) 68, 77
BstMWI GCNNNNNNNGC 2 cut(s) 268, 647
BstNI CCWGG 5 cut(s) 193, 264, 369, 387, 584
BstSCI CCNGG 5 cut(s) 191, 262, 367, 385, 582
BstV1I GCAGC 3 cut(s) 186, 634, 769
BstV2I GAAGAC 2 cut(s) 102, 571
BstX2I RGATCY 2 cut(s) 564, 586
BstYI RGATCY 2 cut(s) 564, 586
BstZI CGGCCG 1 cut(s) 65
BsuRI GGCC 3 cut(s) 67, 129, 505
BtgI CCRYGG 1 cut(s) 609
BtsIMutI CAGTG 1 cut(s) 414
BveI ACCTGC 2 cut(s) 262, 751
CfoI GCGC 1 cut(s) 652
Cfr13I GGNCC 2 cut(s) 503, 686
CseI GACGC 2 cut(s) 350, 614
Csp6I GTAC 1 cut(s) 518
CviAII CATG 5 cut(s) 118, 314, 358, 610, 783
CviQI GTAC 1 cut(s) 518
DdeI CTNAG 1 cut(s) 522
DpnI GATC 5 cut(s) 154, 231, 456, 566, 588
DpnII GATC 5 cut(s) 152, 229, 454, 564, 586
DraI TTTAAA 1 cut(s) 478
DrdI GACNNNNNNGTC 1 cut(s) 44
DriI GACNNNNNGTC 1 cut(s) 19
DseDI GACNNNNNNGTC 1 cut(s) 44
EaeI YGGCCR 2 cut(s) 65, 127
EagI CGGCCG 1 cut(s) 65
Eam1105I GACNNNNNGTC 1 cut(s) 19
Ecl136II GAGCTC 1 cut(s) 174
EclXI CGGCCG 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 609
Eco24I GRGCYC 1 cut(s) 176
Eco31I GGTCTC 1 cut(s) 713
Eco32I GATATC 1 cut(s) 746
Eco47I GGWCC 1 cut(s) 686
Eco52I CGGCCG 1 cut(s) 65
Eco53kI GAGCTC 1 cut(s) 174
Eco57I CTGAAG 1 cut(s) 242
Eco88I CYCGRG 1 cut(s) 169
EcoICRI GAGCTC 1 cut(s) 174
EcoO109I RGGNCCY 1 cut(s) 503
EcoRII CCWGG 5 cut(s) 191, 262, 367, 385, 582
EcoRV GATATC 1 cut(s) 746
EcoT14I CCWWGG 1 cut(s) 609
EcoT38I GRGCYC 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 609
FaeI CATG 5 cut(s) 121, 317, 361, 613, 786
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 1 cut(s) 295
FatI CATG 5 cut(s) 117, 313, 357, 609, 782
FblI GTMKAC 2 cut(s) 26, 217
Fnu4HI GCNGC 5 cut(s) 90, 93, 200, 648, 758
FriOI GRGCYC 1 cut(s) 176
Fsp4HI GCNGC 5 cut(s) 90, 93, 200, 648, 758
FspBI CTAG 1 cut(s) 749
GlaI GCGC 1 cut(s) 651
GluI GCNGC 5 cut(s) 90, 93, 200, 648, 758
GsuI CTGGAG 1 cut(s) 657
HaeIII GGCC 3 cut(s) 67, 129, 505
HapII CCGG 1 cut(s) 81
HgaI GACGC 2 cut(s) 350, 614
HhaI GCGC 1 cut(s) 652
Hin1I GRCGYC 1 cut(s) 104
Hin1II CATG 5 cut(s) 121, 317, 361, 613, 786
Hin6I GCGC 1 cut(s) 650
HinP1I GCGC 1 cut(s) 650
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 3 cut(s) 208, 656, 776
HpaII CCGG 1 cut(s) 81
HphI GGTGA 1 cut(s) 508
Hpy166II GTNNAC 2 cut(s) 27, 218
Hpy188I TCNGA 3 cut(s) 37, 207, 279
Hpy188III TCNNGA 4 cut(s) 431, 590, 674, 712
Hpy8I GTNNAC 2 cut(s) 27, 218
Hpy99I CGWCG 6 cut(s) 28, 106, 109, 152, 191, 216
HpyAV CCTTC 5 cut(s) 493, 535, 698, 721, 761
HpyCH4III ACNGT 2 cut(s) 78, 409
HpyCH4IV ACGT 1 cut(s) 104
HpyCH4V TGCA 3 cut(s) 237, 341, 782
HpyF10VI GCNNNNNNNGC 2 cut(s) 268, 647
HpyF3I CTNAG 1 cut(s) 522
HpySE526I ACGT 1 cut(s) 104
Hsp92I GRCGYC 1 cut(s) 104
Hsp92II CATG 5 cut(s) 121, 317, 361, 613, 786
HspAI GCGC 1 cut(s) 650
Kzo9I GATC 5 cut(s) 152, 229, 454, 564, 586
LmnI GCTCC 2 cut(s) 179, 730
Lsp1109I GCAGC 3 cut(s) 186, 634, 769
LweI GCATC 2 cut(s) 628, 634
MaeI CTAG 1 cut(s) 749
MaeII ACGT 1 cut(s) 104
MaeIII GTNAC 2 cut(s) 83, 657
MalI GATC 5 cut(s) 154, 231, 456, 566, 588
MbiI CCGCTC 1 cut(s) 733
MboI GATC 5 cut(s) 152, 229, 454, 564, 586
MboII GAAGA 5 cut(s) 49, 102, 105, 571, 689
MflI RGATCY 2 cut(s) 564, 586
MhlI GDGCHC 1 cut(s) 176
MluCI AATT 4 cut(s) 291, 446, 473, 617
MlyI GAGTC 3 cut(s) 202, 665, 770
MmeI TCCRAC 2 cut(s) 230, 753
MnlI CCTC 6 cut(s) 171, 178, 375, 418, 681, 729
MseI TTAA 4 cut(s) 294, 381, 471, 477
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 5 cut(s) 193, 264, 369, 387, 584
MvaI CCWGG 5 cut(s) 193, 264, 369, 387, 584
MwoI GCNNNNNNNGC 2 cut(s) 268, 647
NcoI CCATGG 1 cut(s) 609
NdeII GATC 5 cut(s) 152, 229, 454, 564, 586
NlaIII CATG 5 cut(s) 121, 317, 361, 613, 786
NlaIV GGNNCC 4 cut(s) 159, 429, 504, 588
NmeAIII GCCGAG 1 cut(s) 733
NmuCI GTSAC 1 cut(s) 657
PaeR7I CTCGAG 1 cut(s) 169
PcsI WCGNNNNNNNCGW 3 cut(s) 29, 70, 203
PflFI GACNNNGTC 2 cut(s) 31, 106
PfoI TCCNGGA 1 cut(s) 582
PkrI GCNGC 5 cut(s) 91, 94, 201, 649, 759
PleI GAGTC 3 cut(s) 202, 664, 770
PpsI GAGTC 3 cut(s) 202, 664, 770
Psp124BI GAGCTC 1 cut(s) 176
Psp6I CCWGG 5 cut(s) 191, 262, 367, 385, 582
PspGI CCWGG 5 cut(s) 191, 262, 367, 385, 582
PspN4I GGNNCC 4 cut(s) 159, 429, 504, 588
PspPI GGNCC 2 cut(s) 503, 686
PspXI VCTCGAGB 1 cut(s) 169
PsuI RGATCY 2 cut(s) 564, 586
PsyI GACNNNGTC 2 cut(s) 31, 106
RsaI GTAC 1 cut(s) 519
RsaNI GTAC 1 cut(s) 518
SacI GAGCTC 1 cut(s) 176
SalI GTCGAC 1 cut(s) 25
SaqAI TTAA 4 cut(s) 294, 381, 471, 477
SatI GCNGC 5 cut(s) 90, 93, 200, 648, 758
Sau3AI GATC 5 cut(s) 152, 229, 454, 564, 586
Sau96I GGNCC 2 cut(s) 503, 686
SchI GAGTC 3 cut(s) 202, 665, 770
ScrFI CCNGG 5 cut(s) 193, 264, 369, 387, 584
SduI GDGCHC 1 cut(s) 176
SfaNI GCATC 2 cut(s) 628, 634
Sfr274I CTCGAG 1 cut(s) 169
SinI GGWCC 1 cut(s) 686
SlaI CTCGAG 1 cut(s) 169
SmlI CTYRAG 3 cut(s) 162, 169, 653
SmoI CTYRAG 3 cut(s) 162, 169, 653
Sse9I AATT 4 cut(s) 291, 446, 473, 617
SsiI CCGC 3 cut(s) 90, 93, 733
SspMI CTAG 1 cut(s) 749
SstI GAGCTC 1 cut(s) 176
StyD4I CCNGG 5 cut(s) 191, 262, 367, 385, 582
StyI CCWWGG 1 cut(s) 609
TaaI ACNGT 2 cut(s) 78, 409
TaiI ACGT 1 cut(s) 107
TaqI TCGA 4 cut(s) 26, 73, 170, 228
TasI AATT 4 cut(s) 291, 446, 473, 617
TauI GCSGC 2 cut(s) 92, 95
Tru1I TTAA 4 cut(s) 294, 381, 471, 477
Tru9I TTAA 4 cut(s) 294, 381, 471, 477
TscAI CASTG 1 cut(s) 414
TseFI GTSAC 1 cut(s) 657
TseI GCWGC 3 cut(s) 199, 647, 757
Tsp45I GTSAC 1 cut(s) 657
TspDTI ATGAA 3 cut(s) 346, 456, 735
TspGWI ACGGA 3 cut(s) 165, 166, 175
TspRI CASTG 1 cut(s) 414
Tth111I GACNNNGTC 2 cut(s) 31, 106
VpaK11BI GGWCC 1 cut(s) 686
XapI RAATTY 1 cut(s) 473
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XhoI CTCGAG 1 cut(s) 169
XmiI GTMKAC 2 cut(s) 26, 217
XspI CTAG 1 cut(s) 749
ZraI GACGTC 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.