Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011028

Physical Location & Seq

Genomic Coordinates Reverse (-)

11535404 .. 11542405

Gene Structure 7002 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G21710.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3567 bp

ATGGAGTACAACGAATACGAACCTGAACCTCAATATGTAGAAGACGATGACGATGAGGAGATTACCCAGGAGGACGCGTGGGCTGTTATATCTGCTTATTTCGAAGAGAAAGGTCTCGTTCGTCAACAGCTGGATTCGTTTGATGAGTTTATTCAAAATACTATGCAAGAAATCGTTGATGAGTCTGCTGATATCGAGATCCGACCTGAATCTCAGCATAATCCTGGCCATCAGTCCGATTTTGCGGAGACAATCTACAAGATTAGCTTTGGACAGATTTATCTGAGTAAACCTATGATGACAGAGTCTGATGGAGAGACTGCCACCCTGTTCCCTAAGGCTGCAAGGTTGAGAAATCTCACATACTCTGCGCCTTTGTATGTCGATGTTACTAAGAGAGTTATTAAGAAAGGGCATGATGGTGAAGAAGTTACGGAGACACAGGATTTTACTAAAGTTTTCATTGGAAAGGTTCCCATCATGCTCCGGTCTAGTTACTGTACCTTGTTTCAGAACTCGGAGAAAGACCTGACAGAGCTTGGAGAATGTCCTTATGATCAGGGTGGATACTTCATTATTAATGGCAGTGAAAAGGTTCTGATTGCTCAGGAGAAGATGAGTACGAATCATGTTTATGTGTTCAAGAAAAGGCAGCCAAATAAGTATGCTTATGTTGGTGAAGTCCGTTCTATGGCTGAAAACCAAAATAGGCCTCCAAGCACAATGTTTGTGCGTATGCTTGCTCGTGCTAGTGCAAAAGGGGGTTCATCTGGACAGTATATTCGATGTACTCTTCCATACATCAGAACAGAAATTCCTATTATCATAGTATTTCGTGCACTGGGATTTGTTGCCGATAAGGACATATTGGAACACATCTGCTATGATTTTGCCGATACCCAGATGATGGAGTTGCTCAGGCCTTCCTTGGAAGAAGCTTTCGTTATTCAAAATCAGCTGGTTGCACTTGACTATATTGGAAAACGTGGTGCAACTGTTGGTGTAACCAAGGAAAAAAGGATAAAGTATGCTAGAGATATCCTTCAGAAAGAAATGCTTCCTCATGTAGGAATTGGGGAGCATTGCGAGACAAAGAAAGCTTACTATTTTGGGTATATCATACACCGGCTGCTGCTTTGTGCACTTGGCCGAAGGCCAGAAGATGATAGGGATCATTATGGTAACAAAAGGCTGGATCTTGCTGGTCCTTTACTTGGAGGGCTATTTAGAATGCTTTTCCGAAAGTTAACAAGGGATGTGAGATCTTATGTTCAAAAGTGCGTTGACAATGGCAAAGAAGTCAATCTTCAATTTGCCATTAAGGCTAAAACAATTACCTCTGGCCTGAAATATTCTCTTGCTACTGGGAACTGGGGCCAGGCAAACGCTGCTGGCACAAGAGCTGGAGTCTCTCAGGTTCTTAACCGGTTGACATATGCTTCGACTTTGTCACATCTGAGGCGTCTCAATTCTCCAATAGGACGTGAAGGAAAATTGGCAAAACCAAGACAACTGCACAACTCACAGTGGGGAATGATGTGCCCTGCTGAAACACCTGAAGGACAGGCTTGTGGTCTAGTGAAAAACTTGGCTCTCATGGTCTACATAACAGTTGGGTCAGCTGCTTATCCTATATTGGAATTTTTGGAAGAATGGGGTACTGAGAATTTTGAGGAAATATCTCCATCTGTTATACCCCAAGCCACAAAAATCTTTGTCAATGGAATGTGGGTTGGAGTTCATAGAGATCCTGACATGTTGGTGAAAACGTTGAGACGTTTGAGACGAAGGGTTGATGTCAACACTGAAGTTGGTGTAGTTAGAGATATCCGTCTTAAAGAGCTCCGGATATACACTGATTATGGACGTTGTAGCCGTCCGTTGTTTATTGTAGATAATCAGAAGCTCTTAATAAAGAAGAGAGATATCTATGCTCTGCAACAAAGGGAAAGTGCAGAAGAAGATGGTTGGCATCATCTGGTTGCTAAGGGCTTTATAGAATACATAGACACAGAAGAAGAGGAAACGACCATGATTTCCATGACTATCAGTGATCTGGTTCAAGCTAGACTCCGCCCAGAAGAGGCTTATACTGAAAACTACACACATTGTGAGATTCACCCATCTCTGATATTGGGGGTCTGTGCTTCAATCATACCATTTCCTGACCATAATCAGTCACCTCGTAATACATATCAATCTGCCATGGGGAAGCAAGCAATGGGAATTTATGTCACCAACTACCAATTTCGCATGGATACGTTAGCTTATGTTCTCTATTATCCACAAAAGCCTTTGGTTACTACAAGAGCTATGGAGCATCTTCACTTTAGACAACTTCCAGCAGGAATTAATGCTATTGTTGCCATTTCTTGCTATTCTGGATATAATCAAGAAGATTCTGTCATCATGAATCAGTCTTCAATAGATCGTGGTTTCTTTCGATCCTTGTTCTTTCGGTCTTACAGAGATGAGGAGAAAAAAATGGGGACGCTTGTCAAAGAAGACTTTGGGCGCCCAGACAGAGGAAGTACAATGGGTATGCGACATGGTTCTTATGATAAACTGGATGATGATGGTCTTGCGCCTCCTGGTACTAGAGTTTCAGGTGAAGATGTTATCATTGGGAAAACCACTCCAATATCTCAAGATGAGGCTCAAGGACAATCATCCCGATACACTAGACGTGATCATAGTATAAGCTTGCGACATAGTGAAACTGGAATGGTGGATCAGGTGTTATTGACTACAAATGCAGATGGTTTGAGGTTTGTGAAAGTGAGGGTCAGATCCGTGCGGATTCCTCAAATTGGAGACAAATTTAGTAGTAGACACGGTCAGAAGGGAACTGTTGGCATGACCTACACGCAGGAGGATATGCCATGGACGATTGAAGGGGTCACTCCGGACATTATTGTGAATCCACATGCTATTCCGTCTCGAATGACAATTGGACAGCTAATTGAGTGCATCATGGGAAAAGTGGCAGCTCACATGGGAAAAGAAGGAGATGCTACTCCCTTTACAGATGTCACGGTGGATAATATAAGCAAAGCCCTTCATAAGTGTGGGTACCAAATGCGTGGATTTGAGAGAATGTACAATGGTCACACGGGCAGACCACTCACAGCTATGATATTCCTTGGACCAACATATTACCAAAGATTGAAGCATATGGTTGATGACAAGATTCACTCGCGTGGACGAGGTCCGGTGCAAATCTTAACAAGACAACCAGCTGAAGGGCGATCACGTGATGGTGGTCTCCGTTTTGGAGAAATGGAGCGCGATTGCATGATTGCACATGGTGCTGCTCACTTTTTGAAAGAGAGGCTGTTTGATCAGAGTGATGCGTATAGGGTACATGTGTGTGAAGTCTGCGGGCTCATTGCCATTGCAAATCTGAAGAAGAATTCTTTTGAATGCAGAGGTTGCAAGAACAAAACAGATATTGTTCAGGTATACATTCCATATGCTTGCAAATTACTCTTTCAAGAGCTTATGTCAATGGCTATTGCACCAAGGATGCTCACTAAACACCTGAAATCGGCTAAAGGCAGACAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005665 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016591 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030054 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1188

Amino Acids

135.02

Weight (kDa)

7.91

Isoelectric Point (pI)

42.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	38 - 447	7.3e-79	RNA polymerase beta subunit
RNA_pol_Rpb2_2	PF04561	208 - 399	3.5e-55	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	472 - 536	9.9e-25	RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4	PF04566	571 - 632	1.1e-23	RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_5	PF04567	657 - 708	6.4e-17	RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6	PF00562	715 - 1085	9.9e-142	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7	PF04560	1087 - 1178	4.4e-35	RNA polymerase Rpb2, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011028)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21710
fragaria_vesca	FvH4_3g09270
malus_domestica	MD05G1313100.v1.1 MD10G1295300.v1.1
prunus_persica	Prupe.4G046700_v2.0.a1
pyrus_communis	pycom05g29100 pycom05g29110 pycom10g24710 pycom10g24720
rosa_chinensis	RchiOBHm_Chr5g0008301
rosa_laevigata	RLG00000031560
rosa_multiflora	Rmu_sc0021840.1_g000003
rosa_roxburghii	Rroxscaffold_1G00068160
rosa_rugosa	Rorug04G0434800
rosa_samantha	Rh5AG063600 Rh5BG060100 Rh5CG070400 Rh5CG070500 Rh5DG059600
rosa_wichuraiana	Rw5G005730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	3072
AccB1I	GGYRCC	2 cut(s)	2514, 3072
AccB7I	CCANNNNNTGG	1 cut(s)	907
AccI	GTMKAC	3 cut(s)	1602, 2830, 3462
AccII	CGCG	3 cut(s)	77, 3199, 3288
AccIII	TCCGGA	2 cut(s)	1845, 2905
AciI	CCGC	4 cut(s)	245, 2074, 2797, 3381
AclI	AACGTT	1 cut(s)	1769
AclWI	GGATC	7 cut(s)	193, 1179, 1203, 1742, 2439, 2739, 2784
AcoI	YGGCCR	2 cut(s)	226, 1147
AcsI	RAATTY	6 cut(s)	813, 1640, 1666, 2226, 2819, 3412
AcuI	CTGAAG	5 cut(s)	1028, 1578, 1827, 3261, 3425
AcvI	CACGTG	1 cut(s)	3254
AcyI	GRCGYC	2 cut(s)	1462, 2515
AdeI	CACNNNGTG	2 cut(s)	2111, 3308
AfiI	CCNNNNNNNGG	9 cut(s)	691, 907, 1067, 1214, 2083, 2525, 2810, 3242, 3547
AflIII	ACRYGT	3 cut(s)	75, 1755, 3364
AgeI	ACCGGT	1 cut(s)	1425
AjiI	CACGTC	2 cut(s)	1484, 2687
AjnI	CCWGG	4 cut(s)	66, 223, 1377, 2590
AleI	CACNNNNGTG	1 cut(s)	3198
AloI	GAACNNNNNNTCC	2 cut(s)	799, 831
Alw21I	GWGCWC	3 cut(s)	841, 1144, 1845
Alw44I	GTGCAC	2 cut(s)	837, 1140
AlwI	GGATC	7 cut(s)	193, 1179, 1203, 1742, 2439, 2739, 2784
AlwNI	CAGNNNCTG	1 cut(s)	308
Aor13HI	TCCGGA	2 cut(s)	1845, 2905
AoxI	GGCC	7 cut(s)	226, 710, 920, 1147, 1154, 1342, 1375
ApaLI	GTGCAC	2 cut(s)	837, 1140
ApeKI	GCWGC	8 cut(s)	341, 652, 1129, 1132, 1388, 1622, 2987, 3311
ApoI	RAATTY	6 cut(s)	813, 1640, 1666, 2226, 2819, 3412
ArsI	GACNNNNNNTTYG	4 cut(s)	765, 797, 1423, 1455
AseI	ATTAAT	2 cut(s)	579, 2352
AsiGI	ACCGGT	1 cut(s)	1425
Asp700I	GAANNNNTTC	2 cut(s)	594, 1056
Asp718I	GGTACC	1 cut(s)	3072
AspLEI	GCGC	4 cut(s)	373, 2517, 2587, 3288
AspS9I	GGNCC	4 cut(s)	1205, 1375, 3146, 3209
AsuHPI	GGTGA	7 cut(s)	434, 689, 1774, 2111, 2172, 2227, 2621
AsuII	TTCGAA	1 cut(s)	102
AvaII	GGWCC	3 cut(s)	1205, 3146, 3209
AxyI	CCTNAGG	1 cut(s)	336
BaeGI	GKGCMC	3 cut(s)	841, 1144, 1544
BaeI	ACNNNNGTAYC	4 cut(s)	2586, 2619, 3353, 3386
BalI	TGGCCA	1 cut(s)	228
BanI	GGYRCC	2 cut(s)	2514, 3072
BanII	GRGCYC	2 cut(s)	1845, 3387
BauI	CACGAG	1 cut(s)	744
BbrPI	CACGTG	1 cut(s)	3254
BbsI	GAAGAC	3 cut(s)	48, 2412, 2511
Bbv12I	GWGCWC	3 cut(s)	841, 1144, 1845
BbvI	GCAGC	8 cut(s)	328, 664, 1116, 1119, 1375, 1609, 2999, 3298
BceAI	ACGGC	1 cut(s)	1860
BciT130I	CCWGG	4 cut(s)	68, 225, 1379, 2592
BciVI	GTATCC	2 cut(s)	560, 2251
BclI	TGATCA	3 cut(s)	556, 2689, 3340
BfaI	CTAG	7 cut(s)	492, 750, 1032, 1577, 2067, 2598, 2682
BfoI	RGCGCY	1 cut(s)	2518
BfuI	GTATCC	2 cut(s)	560, 2251
BglI	GCCNNNNNGGC	1 cut(s)	1322
BglII	AGATCT	1 cut(s)	1262
BisI	GCNGC	8 cut(s)	342, 653, 1130, 1133, 1389, 1623, 2988, 3312
BlsI	GCNGC	8 cut(s)	343, 654, 1131, 1134, 1390, 1624, 2989, 3313
Bme1390I	CCNGG	4 cut(s)	68, 225, 1379, 2592
Bme18I	GGWCC	3 cut(s)	1205, 3146, 3209
BmgBI	CACGTC	2 cut(s)	1484, 2687
BmgT120I	GGNCC	4 cut(s)	1205, 1375, 3146, 3209
BmiI	GGNNCC	4 cut(s)	474, 1376, 2516, 3074
BmrFI	CCNGG	4 cut(s)	68, 225, 1379, 2592
BmrI	ACTGGG	3 cut(s)	851, 1374, 1381
BmsI	GCATC	6 cut(s)	1981, 2329, 2979, 3001, 3340, 3516
BmuI	ACTGGG	3 cut(s)	851, 1374, 1381
BoxI	GACNNNNGTC	1 cut(s)	2495
BpiI	GAAGAC	3 cut(s)	48, 2412, 2511
BpmI	CTGGAG	1 cut(s)	1425
Bpu10I	CCTNAGC	3 cut(s)	606, 917, 1986
Bpu14I	TTCGAA	1 cut(s)	102
BpuEI	CTTGAG	2 cut(s)	2631, 2643
BsaAI	YACGTR	1 cut(s)	3254
BsaBI	GATNNNNATC	1 cut(s)	1170
BsaHI	GRCGYC	2 cut(s)	1462, 2515
BsaI	GGTCTC	2 cut(s)	119, 3269
BsaJI	CCNNGG	7 cut(s)	66, 927, 1008, 2205, 2882, 3142, 3521
BsaWI	WCCGGW	5 cut(s)	486, 1425, 1845, 2905, 3211
BsaXI	ACNNNNNCTCC	2 cut(s)	62, 92
Bsc4I	CCNNNNNNNGG	9 cut(s)	691, 907, 1067, 1214, 2083, 2525, 2810, 3242, 3547
Bse118I	RCCGGY	2 cut(s)	1125, 1425
Bse1I	ACTGG	5 cut(s)	846, 1369, 1376, 2571, 2725
Bse21I	CCTNAGG	1 cut(s)	336
Bse3DI	GCAATG	4 cut(s)	1081, 2226, 3387, 3393
Bse8I	GATNNNNATC	1 cut(s)	1170
BseAI	TCCGGA	2 cut(s)	1845, 2905
BseBI	CCWGG	4 cut(s)	68, 225, 1379, 2592
BseDI	CCNNGG	7 cut(s)	66, 927, 1008, 2205, 2882, 3142, 3521
BseGI	GGATG	4 cut(s)	1261, 2575, 2669, 3531
BseJI	GATNNNNATC	1 cut(s)	1170
BseLI	CCNNNNNNNGG	9 cut(s)	691, 907, 1067, 1214, 2083, 2525, 2810, 3242, 3547
BseMI	GCAATG	4 cut(s)	1081, 2226, 3387, 3393
BseMII	CTCAG	7 cut(s)	227, 275, 620, 931, 1427, 1448, 1653
BseNI	ACTGG	5 cut(s)	846, 1369, 1376, 2571, 2725
BseRI	GAGGAG	2 cut(s)	71, 2489
BseSI	GKGCMC	3 cut(s)	841, 1144, 1544
BseXI	GCAGC	8 cut(s)	328, 664, 1116, 1119, 1375, 1609, 2999, 3298
BsgI	GTGCAG	2 cut(s)	1499, 1974
Bsh1236I	CGCG	3 cut(s)	77, 3199, 3288
BshFI	GGCC	7 cut(s)	228, 712, 922, 1149, 1156, 1344, 1377
BshNI	GGYRCC	2 cut(s)	2514, 3072
BshTI	ACCGGT	1 cut(s)	1425
BsiHKAI	GWGCWC	3 cut(s)	841, 1144, 1845
BsiSI	CCGG	6 cut(s)	487, 1126, 1426, 1846, 2906, 3212
BslFI	GGGAC	1 cut(s)	2503
BslI	CCNNNNNNNGG	9 cut(s)	691, 907, 1067, 1214, 2083, 2525, 2810, 3242, 3547
BsmBI	CGTCTC	4 cut(s)	1469, 1768, 1777, 2943
BsmFI	GGGAC	1 cut(s)	2503
BsmI	GAATGC	2 cut(s)	1236, 3428
BsnI	GGCC	7 cut(s)	228, 712, 922, 1149, 1156, 1344, 1377
Bso31I	GGTCTC	2 cut(s)	119, 3269
Bsp119I	TTCGAA	1 cut(s)	102
Bsp1286I	GDGCHC	5 cut(s)	841, 1144, 1544, 1845, 3387
Bsp13I	TCCGGA	2 cut(s)	1845, 2905
Bsp1407I	TGTACA	1 cut(s)	3099
Bsp19I	CCATGG	2 cut(s)	2205, 2882
BspACI	CCGC	4 cut(s)	245, 2074, 2797, 3381
BspANI	GGCC	7 cut(s)	228, 712, 922, 1149, 1156, 1344, 1377
BspCNI	CTCAG	7 cut(s)	226, 276, 619, 930, 1426, 1449, 1654
BspEI	TCCGGA	2 cut(s)	1845, 2905
BspFNI	CGCG	3 cut(s)	77, 3199, 3288
BspHI	TCATGA	1 cut(s)	2409
BspLI	GGNNCC	4 cut(s)	474, 1376, 2516, 3074
BspPI	GGATC	7 cut(s)	193, 1179, 1203, 1742, 2439, 2739, 2784
BspT104I	TTCGAA	1 cut(s)	102
BspT107I	GGYRCC	2 cut(s)	2514, 3072
BspTNI	GGTCTC	2 cut(s)	119, 3269
BsrDI	GCAATG	4 cut(s)	1081, 2226, 3387, 3393
BsrFI	RCCGGY	2 cut(s)	1125, 1425
BsrGI	TGTACA	1 cut(s)	3099
BsrI	ACTGG	5 cut(s)	846, 1369, 1376, 2571, 2725
BssAI	RCCGGY	2 cut(s)	1125, 1425
BssECI	CCNNGG	7 cut(s)	66, 927, 1008, 2205, 2882, 3142, 3521
BssNAI	GTATAC	1 cut(s)	3463
BssNI	GRCGYC	2 cut(s)	1462, 2515
BssSI	CACGAG	1 cut(s)	744
BssT1I	CCWWGG	6 cut(s)	927, 1008, 2205, 2882, 3142, 3521
Bst1107I	GTATAC	1 cut(s)	3463
Bst2BI	CACGAG	1 cut(s)	744
Bst2UI	CCWGG	4 cut(s)	68, 225, 1379, 2592
Bst4CI	ACNGT	9 cut(s)	500, 777, 997, 1527, 1612, 2837, 2851, 3037, 3564
Bst6I	CTCTTC	5 cut(s)	99, 798, 1913, 2013, 2076
BstACI	GRCGYC	2 cut(s)	1462, 2515
BstAPI	GCANNNNNTGC	3 cut(s)	1388, 3308, 3432
BstAUI	TGTACA	1 cut(s)	3099
BstBAI	YACGTR	1 cut(s)	3254
BstBI	TTCGAA	1 cut(s)	102
BstC8I	GCNNGC	6 cut(s)	741, 1393, 2217, 2705, 3383, 3478
BstDSI	CCRYGG	2 cut(s)	2205, 2882
BstENI	CCTNNNNNAGG	1 cut(s)	1065
BstF5I	GGATG	4 cut(s)	1261, 2575, 2669, 3531
BstFNI	CGCG	3 cut(s)	77, 3199, 3288
BstH2I	RGCGCY	1 cut(s)	2518
BstHHI	GCGC	4 cut(s)	373, 2517, 2587, 3288
BstMWI	GCNNNNNNNGC	5 cut(s)	1322, 1388, 2363, 3308, 3432
BstNI	CCWGG	4 cut(s)	68, 225, 1379, 2592
BstNSI	RCATGY	3 cut(s)	1759, 2930, 3368
BstPAI	GACNNNNGTC	1 cut(s)	2495
BstSCI	CCNGG	4 cut(s)	66, 223, 1377, 2590
BstSLI	GKGCMC	3 cut(s)	841, 1144, 1544
BstUI	CGCG	3 cut(s)	77, 3199, 3288
BstV1I	GCAGC	8 cut(s)	328, 664, 1116, 1119, 1375, 1609, 2999, 3298
BstV2I	GAAGAC	3 cut(s)	48, 2412, 2511
BstX2I	RGATCY	5 cut(s)	198, 1195, 1262, 1747, 2789
BstXI	CCANNNNNNTGG	1 cut(s)	3083
BstYI	RGATCY	5 cut(s)	198, 1195, 1262, 1747, 2789
BstZ17I	GTATAC	1 cut(s)	3463
Bsu36I	CCTNAGG	1 cut(s)	336
BsuI	GTATCC	2 cut(s)	560, 2251
BsuRI	GGCC	7 cut(s)	228, 712, 922, 1149, 1156, 1344, 1377
BtgI	CCRYGG	2 cut(s)	2205, 2882
BtrI	CACGTC	2 cut(s)	1484, 2687
BtsCI	GGATG	4 cut(s)	1261, 2575, 2669, 3531
BtsI	GCAGTG	1 cut(s)	592
BtsIMutI	CAGTG	6 cut(s)	592, 839, 1532, 1803, 1854, 2056
Cac8I	GCNNGC	6 cut(s)	741, 1393, 2217, 2705, 3383, 3478
CaiI	CAGNNNCTG	1 cut(s)	308
CciI	TCATGA	1 cut(s)	2409
CfoI	GCGC	4 cut(s)	373, 2517, 2587, 3288
Cfr10I	RCCGGY	2 cut(s)	1125, 1425
Cfr13I	GGNCC	4 cut(s)	1205, 1375, 3146, 3209
CseI	GACGC	3 cut(s)	83, 1451, 2500
CspAI	ACCGGT	1 cut(s)	1425
DinI	GGCGCC	1 cut(s)	2516
DraIII	CACNNNGTG	2 cut(s)	2111, 3308
EaeI	YGGCCR	2 cut(s)	226, 1147
Eam1104I	CTCTTC	5 cut(s)	99, 798, 1913, 2013, 2076
EarI	CTCTTC	5 cut(s)	99, 798, 1913, 2013, 2076
EciI	GGCGGA	1 cut(s)	2063
Ecl136II	GAGCTC	1 cut(s)	1843
Eco130I	CCWWGG	6 cut(s)	927, 1008, 2205, 2882, 3142, 3521
Eco147I	AGGCCT	2 cut(s)	712, 922
Eco24I	GRGCYC	2 cut(s)	1845, 3387
Eco31I	GGTCTC	2 cut(s)	119, 3269
Eco32I	GATATC	4 cut(s)	193, 1039, 1828, 1927
Eco47I	GGWCC	3 cut(s)	1205, 3146, 3209
Eco53kI	GAGCTC	1 cut(s)	1843
Eco57I	CTGAAG	5 cut(s)	1028, 1578, 1827, 3261, 3425
Eco72I	CACGTG	1 cut(s)	3254
Eco81I	CCTNAGG	1 cut(s)	336
EcoICRI	GAGCTC	1 cut(s)	1843
EcoNI	CCTNNNNNAGG	1 cut(s)	1065
EcoRI	GAATTC	1 cut(s)	3412
EcoRII	CCWGG	4 cut(s)	66, 223, 1377, 2590
EcoRV	GATATC	4 cut(s)	193, 1039, 1828, 1927
EcoT14I	CCWWGG	6 cut(s)	927, 1008, 2205, 2882, 3142, 3521
EcoT38I	GRGCYC	2 cut(s)	1845, 3387
EgeI	GGCGCC	1 cut(s)	2516
EheI	GGCGCC	1 cut(s)	2516
ErhI	CCWWGG	6 cut(s)	927, 1008, 2205, 2882, 3142, 3521
Esp3I	CGTCTC	4 cut(s)	1469, 1768, 1777, 2943
FalI	AAGNNNNNCTT	6 cut(s)	251, 283, 1041, 1073, 1290, 1322
FaqI	GGGAC	1 cut(s)	2503
FauI	CCCGC	1 cut(s)	3374
FauNDI	CATATG	3 cut(s)	1435, 3174, 3472
FbaI	TGATCA	3 cut(s)	556, 2689, 3340
FblI	GTMKAC	3 cut(s)	1602, 2830, 3462
Fnu4HI	GCNGC	8 cut(s)	342, 653, 1130, 1133, 1389, 1623, 2988, 3312
FokI	GGATG	4 cut(s)	1268, 2582, 2656, 3538
FriOI	GRGCYC	2 cut(s)	1845, 3387
Fsp4HI	GCNGC	8 cut(s)	342, 653, 1130, 1133, 1389, 1623, 2988, 3312
FspBI	CTAG	7 cut(s)	492, 750, 1032, 1577, 2067, 2598, 2682
GlaI	GCGC	4 cut(s)	372, 2516, 2586, 3287
GluI	GCNGC	8 cut(s)	342, 653, 1130, 1133, 1389, 1623, 2988, 3312
GsuI	CTGGAG	1 cut(s)	1425
HaeII	RGCGCY	1 cut(s)	2518
HaeIII	GGCC	7 cut(s)	228, 712, 922, 1149, 1156, 1344, 1377
HapII	CCGG	6 cut(s)	487, 1126, 1426, 1846, 2906, 3212
HgaI	GACGC	3 cut(s)	83, 1451, 2500
HhaI	GCGC	4 cut(s)	373, 2517, 2587, 3288
Hin1I	GRCGYC	2 cut(s)	1462, 2515
Hin6I	GCGC	4 cut(s)	371, 2515, 2585, 3286
HinP1I	GCGC	4 cut(s)	371, 2515, 2585, 3286
HincII	GTYRAC	5 cut(s)	125, 1248, 1285, 1431, 1801
HindII	GTYRAC	5 cut(s)	125, 1248, 1285, 1431, 1801
HindIII	AAGCTT	3 cut(s)	936, 1098, 2701
HpaI	GTTAAC	1 cut(s)	1248
HpaII	CCGG	6 cut(s)	487, 1126, 1426, 1846, 2906, 3212
HphI	GGTGA	7 cut(s)	434, 689, 1774, 2111, 2172, 2227, 2621
HpyCH4III	ACNGT	9 cut(s)	500, 777, 997, 1527, 1612, 2837, 2851, 3037, 3564
HpyCH4IV	ACGT	8 cut(s)	985, 1483, 1769, 1777, 1867, 2261, 2686, 3253
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1322, 1388, 2363, 3308, 3432
HpySE526I	ACGT	8 cut(s)	985, 1483, 1769, 1777, 1867, 2261, 2686, 3253
Hsp92I	GRCGYC	2 cut(s)	1462, 2515
HspAI	GCGC	4 cut(s)	371, 2515, 2585, 3286
KasI	GGCGCC	1 cut(s)	2514
Kpn2I	TCCGGA	2 cut(s)	1845, 2905
KpnI	GGTACC	1 cut(s)	3076
Ksp22I	TGATCA	3 cut(s)	556, 2689, 3340
KspAI	GTTAAC	1 cut(s)	1248
LmnI	GCTCC	5 cut(s)	489, 1078, 1848, 2317, 3283
Lsp1109I	GCAGC	8 cut(s)	328, 664, 1116, 1119, 1375, 1609, 2999, 3298
LweI	GCATC	6 cut(s)	1981, 2329, 2979, 3001, 3340, 3516
MaeI	CTAG	7 cut(s)	492, 750, 1032, 1577, 2067, 2598, 2682
MaeII	ACGT	8 cut(s)	985, 1483, 1769, 1777, 1867, 2261, 2686, 3253
MfeI	CAATTG	1 cut(s)	2949
MflI	RGATCY	5 cut(s)	198, 1195, 1262, 1747, 2789
MhlI	GDGCHC	5 cut(s)	841, 1144, 1544, 1845, 3387
MlsI	TGGCCA	1 cut(s)	228
MluI	ACGCGT	1 cut(s)	75
MluNI	TGGCCA	1 cut(s)	228
Mly113I	GGCGCC	1 cut(s)	2515
MlyI	GAGTC	4 cut(s)	191, 314, 1416, 2064
MmeI	TCCRAC	2 cut(s)	226, 1714
Mox20I	TGGCCA	1 cut(s)	228
MroI	TCCGGA	2 cut(s)	1845, 2905
MroXI	GAANNNNTTC	2 cut(s)	594, 1056
MscI	TGGCCA	1 cut(s)	228
MseI	TTAA	9 cut(s)	405, 579, 1247, 1320, 1422, 1836, 1910, 2352, 3224
MslI	CAYNNNNRTG	8 cut(s)	420, 633, 1760, 2915, 3066, 3131, 3198, 3369
Msp20I	TGGCCA	1 cut(s)	228
MspA1I	CMGCKG	4 cut(s)	130, 958, 1622, 3239
MspI	CCGG	6 cut(s)	487, 1126, 1426, 1846, 2906, 3212
MspR9I	CCNGG	4 cut(s)	68, 225, 1379, 2592
MunI	CAATTG	1 cut(s)	2949
Mva1269I	GAATGC	2 cut(s)	1236, 3428
MvaI	CCWGG	4 cut(s)	68, 225, 1379, 2592
MvnI	CGCG	3 cut(s)	77, 3199, 3288
MwoI	GCNNNNNNNGC	5 cut(s)	1322, 1388, 2363, 3308, 3432
NarI	GGCGCC	1 cut(s)	2515
NcoI	CCATGG	2 cut(s)	2205, 2882
NdeI	CATATG	3 cut(s)	1435, 3174, 3472
NlaIV	GGNNCC	4 cut(s)	474, 1376, 2516, 3074
NmuCI	GTSAC	6 cut(s)	1449, 2178, 2233, 2899, 3031, 3107
NspI	RCATGY	3 cut(s)	1759, 2930, 3368
NspV	TTCGAA	1 cut(s)	102
OliI	CACNNNNGTG	1 cut(s)	3198
PagI	TCATGA	1 cut(s)	2409
PceI	AGGCCT	2 cut(s)	712, 922
PciI	ACATGT	2 cut(s)	1755, 3364
PcsI	WCGNNNNNNNCGW	2 cut(s)	1783, 1873
PctI	GAATGC	2 cut(s)	1236, 3428
PdmI	GAANNNNTTC	2 cut(s)	594, 1056
PfeI	GAWTC	9 cut(s)	134, 209, 625, 2116, 2399, 2413, 2800, 2920, 3190
PflFI	GACNNNGTC	4 cut(s)	304, 1447, 2835, 3207
PflMI	CCANNNNNTGG	1 cut(s)	907
PinAI	ACCGGT	1 cut(s)	1425
PkrI	GCNGC	8 cut(s)	343, 654, 1131, 1134, 1390, 1624, 2989, 3313
PleI	GAGTC	4 cut(s)	190, 313, 1415, 2064
PluTI	GGCGCC	1 cut(s)	2518
PmaCI	CACGTG	1 cut(s)	3254
PmlI	CACGTG	1 cut(s)	3254
PpsI	GAGTC	4 cut(s)	190, 313, 1415, 2064
Ppu21I	YACGTR	1 cut(s)	3254
PscI	ACATGT	2 cut(s)	1755, 3364
PshAI	GACNNNNGTC	1 cut(s)	2495
PshBI	ATTAAT	2 cut(s)	579, 2352
Psp124BI	GAGCTC	1 cut(s)	1845
Psp1406I	AACGTT	1 cut(s)	1769
Psp6I	CCWGG	4 cut(s)	66, 223, 1377, 2590
PspCI	CACGTG	1 cut(s)	3254
PspGI	CCWGG	4 cut(s)	66, 223, 1377, 2590
PspN4I	GGNNCC	4 cut(s)	474, 1376, 2516, 3074
PspPI	GGNCC	4 cut(s)	1205, 1375, 3146, 3209
PstNI	CAGNNNCTG	1 cut(s)	308
PsuI	RGATCY	5 cut(s)	198, 1195, 1262, 1747, 2789
PsyI	GACNNNGTC	4 cut(s)	304, 1447, 2835, 3207
PvuII	CAGCTG	4 cut(s)	130, 958, 1622, 3239
RseI	CAYNNNNRTG	8 cut(s)	420, 633, 1760, 2915, 3066, 3131, 3198, 3369
SacI	GAGCTC	1 cut(s)	1845
SaqAI	TTAA	9 cut(s)	405, 579, 1247, 1320, 1422, 1836, 1910, 2352, 3224
SatI	GCNGC	8 cut(s)	342, 653, 1130, 1133, 1389, 1623, 2988, 3312
Sau96I	GGNCC	4 cut(s)	1205, 1375, 3146, 3209
SchI	GAGTC	4 cut(s)	191, 314, 1416, 2064
ScrFI	CCNGG	4 cut(s)	68, 225, 1379, 2592
SduI	GDGCHC	5 cut(s)	841, 1144, 1544, 1845, 3387
SfaNI	GCATC	6 cut(s)	1981, 2329, 2979, 3001, 3340, 3516
SfoI	GGCGCC	1 cut(s)	2516
SfuI	TTCGAA	1 cut(s)	102
SinI	GGWCC	3 cut(s)	1205, 3146, 3209
SmiMI	CAYNNNNRTG	8 cut(s)	420, 633, 1760, 2915, 3066, 3131, 3198, 3369
SmlI	CTYRAG	2 cut(s)	2646, 2658
SmoI	CTYRAG	2 cut(s)	2646, 2658
SseBI	AGGCCT	2 cut(s)	712, 922
SsiI	CCGC	4 cut(s)	245, 2074, 2797, 3381
SspDI	GGCGCC	1 cut(s)	2514
SspI	AATATT	1 cut(s)	1352
SspMI	CTAG	7 cut(s)	492, 750, 1032, 1577, 2067, 2598, 2682
SstI	GAGCTC	1 cut(s)	1845
StuI	AGGCCT	2 cut(s)	712, 922
StyD4I	CCNGG	4 cut(s)	66, 223, 1377, 2590
StyI	CCWWGG	6 cut(s)	927, 1008, 2205, 2882, 3142, 3521
TaaI	ACNGT	9 cut(s)	500, 777, 997, 1527, 1612, 2837, 2851, 3037, 3564
TaiI	ACGT	8 cut(s)	988, 1486, 1772, 1780, 1870, 2264, 2689, 3256
TaqI	TCGA	7 cut(s)	102, 195, 384, 784, 1442, 2443, 2941
TaqII	GACCGA	1 cut(s)	2448
TatI	WGTACW	4 cut(s)	6, 788, 2531, 3099
TfiI	GAWTC	9 cut(s)	134, 209, 625, 2116, 2399, 2413, 2800, 2920, 3190
Tru1I	TTAA	9 cut(s)	405, 579, 1247, 1320, 1422, 1836, 1910, 2352, 3224
Tru9I	TTAA	9 cut(s)	405, 579, 1247, 1320, 1422, 1836, 1910, 2352, 3224
TscAI	CASTG	6 cut(s)	592, 846, 1532, 1810, 1861, 2056
TseFI	GTSAC	6 cut(s)	1449, 2178, 2233, 2899, 3031, 3107
TseI	GCWGC	8 cut(s)	341, 652, 1129, 1132, 1388, 1622, 2987, 3311
Tsp45I	GTSAC	6 cut(s)	1449, 2178, 2233, 2899, 3031, 3107
TspDTI	ATGAA	6 cut(s)	451, 562, 756, 1730, 2426, 3050
TspGWI	ACGGA	7 cut(s)	449, 674, 1820, 1869, 2782, 2925, 3257
TspRI	CASTG	6 cut(s)	592, 846, 1532, 1810, 1861, 2056
Tth111I	GACNNNGTC	4 cut(s)	304, 1447, 2835, 3207
Van91I	CCANNNNNTGG	1 cut(s)	907
VneI	GTGCAC	2 cut(s)	837, 1140
VpaK11BI	GGWCC	3 cut(s)	1205, 3146, 3209
VspI	ATTAAT	2 cut(s)	579, 2352
XagI	CCTNNNNNAGG	1 cut(s)	1065
XapI	RAATTY	6 cut(s)	813, 1640, 1666, 2226, 2819, 3412
XceI	RCATGY	3 cut(s)	1759, 2930, 3368
XmiI	GTMKAC	3 cut(s)	1602, 2830, 3462
XmnI	GAANNNNTTC	2 cut(s)	594, 1056
XspI	CTAG	7 cut(s)	492, 750, 1032, 1577, 2067, 2598, 2682

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.