Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0011028

Physical Location & Seq

Genomic Coordinates Forward (+)

44379157 .. 44385998

Gene Structure 6842 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1313100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3582 bp

ATGGAGGAGGAGCACGAGTACGACGATCAGCAGTACATGGACGACGACGATGAAGATGAAATCACGCAGGAGGACGCGTGGGCCGTCATCTCCGCCTACTTCGAGGAGAAGGGCTTGGTGCGCCAGCAGCTCGACTCGTTCGATGAGTTCATTCAGAACACAATGCAGGAGATTGTCGACGAGTCGGCCGATATCGAGATTCGGCCCGAGTCGCAGCACAACCCGGGGCACCAGCCCGATTTCGCCGAGACGGTATGCAAGATTAGCTTTGGTCAGATTTATTTGAGTAAGCCCATGATGACCGAATCCGATGGAGAAACTGCAACCTTATTTCCCAAAGCTGCAAGGCTTAGAAATCTGACGTACTCAGCACCCTTGTATGTTGATGTCTCAAAGAGGATGATAAAGAAAGGGCACGATGGCGAGGAACTCACTGAGACCCAGGATTTCACCAAAGTATTCATTGGGAAGGTTCCTATAATGCTTCGGTCTAGTTATTGCACGCTGTACCAGAATTCGGAGAAAGATTTAACTGAGCTGGGAGAGTGTCCATACGATCAAGGTGGATATTTTATTATAAATGGGAGTGAAAAGGTTCTTATTGCTCAAGAGAAGATGAGCACCAACCATGTCTATGTGTTCAAGAAGAGGCAGCCTAACAAGTACGCCTATGTGGCTGAAGTTCGGTCTATGGCGGAGTCTCAAAACAGGCCGCCCAGTACCATGTTTGTTCGGATGCTTGCTCGGACTAGTTCTAAAGGGGGTTCTTCGGGACAGTACATCAAAGCTACTCTTCCGTATATTAAAGGTGAAATTCCTATTATTATTGTGTTCCGGGCTCTCGGGTTTGTAGCGGATAAAGACATCCTAGAGCACATATGTTATGACTTCTCTGATACTCAAATGATGGAGCTGCTTAGACCTTCTCTGGAAGAAGCATTTGTGATTCAGAATCAACAGGTTGCACTAGATTATATTGGAAAGAGAGGTGCAAATGTTGGTGTAAGTAGAGACAAGAGAATCAAGTATGCGAAAGAGATTCTACAAAAGGAAATGCTTCCACACGTTGGTGTTGGAGAATTTTGTGAGACAAAGAAAGCTTATTATTTTGGATATATCGTCCACAGGCTTCTACTATGTGCCCTAGGACGGAGGCCAGAAGATGACAGGGATCATTATGGCAACAAGAGGCTTGATCTTGCTGGTCCCTTACTCGGAGGCTTGTTTCGAAATCTATTCAGGAAGTTGACTAAGGATGTGAGAGCTTATGCACAGAAGTGTGTTGACAATGGAAAGGATCTTAATCTGCAATTTGCCATCAAAGCTAAAACCATTACGAGTGGCCTCAAATATTCACTTGCCACTGGAAACTGGGGGCAAGCAAATGCAGCTGGTACAAGGGCTGGAGTGTCGCAGGTGTTAAATCGTTTGACATACGCATCCACCCTCTCACACTTGAGAAGACTGAATTCTCCAATAGGGCGTGAAGGGAAATTGGCTAAACCGCGTCAGCTGCATAATTCGCAATGGGGAATGATGTGCCCAGCTGAAACACCTGAAGGGCAAGCGTGCGGGCTTGTAAAGAATCTTGCATTGATGGTATACATAACGGTAGGATCAGCTGCATATCCAATCTTGGAATTTTTGGAAGAATGGGGTACAGAAAACTTTGAGGAAATTTCTCCTGCGGTTATACCCCAAGCTACAAAAATTTTTGTTAACGGCTGTTGGGTTGGAATCCATCGTGATCCTGAAATGTTGGTGAGGACATTGAGAAAGCTAAGGCGACGGGTTGATGTCAACACTGAAGTTGGGGTTGTGAGAGATATCCGTCTGAAAGAATTGCGGATATATACTGACTATGGACGTTGCAGTCGTCCATTATTCATTGTGGATAAACAGAGGCTTCTCATAAAGAAGAGGGATATTCATGCATTGCAGCAGAGGGAAAGCCCTGAAGATGCTGGTTGGCATGACCTTGTAGCAAAGGGATATATTGAATATATTGACACTGAAGAAGAGGAGACTACAATGATTTCCATGACAATTAATGATCTTGTCCAAGCAAGACTCAATCCTGAGGAGGCATATTCGGACACTTATACTCATTGTGAAATCCACCCATCACTTATTTTGGGTGTTTGTGCTTCTATTATACCGTTTCCCGATCATAATCAGTCTCCACGTAATACATATCAATCTGCTATGGGGAAGCAAGCTATGGGGATTTATGTCACCAATTATCAGTTCCGGATGGATACATTGGCGTATGTTTTATACTACCCTCAGAAACCTCTTGTCACCACAAGAGCCATGGAGCATCTCCACTTCAGGCAGCTTCCCGCCGGCATCAATGCTATTGTTGCCATCTCCTGTTATTCTGGTTATAACCAGGAAGATTCTGTCATTATGAATCAATCCTCTGTGGACCGTGGATTCTTCCGGTCACTATTCTTCCGATCTTATAGGGACGAGGAGAAAAAGATGGGGACCCTTGTCAAAGAGGATTTTGGGCGTCCTGACAGAGCAAATACTATGGGTATGCGACATGGTTCTTATGACAAATTGGATGATGATGGTCTTGCTCCTCCTGGAACTAGGGTTTCAGGTGAGGATGTTATCATAGGAAAGACTACACCCATGGCTCAGGATGAGGCTCCAGGGCAAGCTACATCGCGTTACACACGGCGTGACCACAGCATAAGCTTGCGCCACAGTGAAACCGGCATAGTTGATCAGGTTCTACTGACGACCAATGCCGATGGGTTGCGGTTTGTTAAAGTACGGGTGAGGTCTGTCAGAATACCTCAAATTGGTGATAAATTTAGTAGCAGGCATGGACAGAAGGGGACAGTGGGTATGACTTATACCCAGGAAGACATGCCGTGGACTGTGGAAGGGGTGACCCCCGATATAATCGTGAACCCGCATGCTATTCCTTCTCGGATGACAATTGGTCAGCTCATTGAGTGTATTATGGGGAAGGTTGCAGCTCACATGGGAAAGGAAGGAGATGCTACACCTTTTACAGATGTCACGGTGGACAATATCAGTAAAGCTCTTCACAGTTGTGGGTATCAAATGCGTGGATTTGAGACCATGTATAATGGTCACACGGGCAGGCGGCTCAGCGCTATGATATTCCTTGGCCCCACATACTACCAGAGACTGAAGCATATGGTCGACGATAAGATCCATTCACGTGGACGGGGTCCAGTGCAGATCCTCACAAGGCAGCCAGCTGAGGGACGATCTCGTGATGGTGGTCTACGCTTCGGGGAAATGGAACGAGATTGCATGATTGCCCATGGTGCTGCTCACTTTCTGAAAGAAAGATTATTTGACCAGAGTGATGCATATAGGGTTCATGTCTGTGAGCGTTGTGGGTTAATAGCTATTGCAAATCTCAAGAAGAATTCGTTTGAGTGCAGGGGTTGCAAGAACAAAACCGACATTGTTCAGGTTTACATTCCTTATGCCTGCAAGCTGCTGTTCCAAGAGCTCATGTCGATGGCCATAGCGCCAAGAATGCTCACAAAGGACATCAAGCCTGCCAAAAACCAAAAGAAGAAAGGAGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005665 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016591 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030054 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1194

Amino Acids

135.23

Weight (kDa)

7.73

Isoelectric Point (pI)

41.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	38 - 447	3.2e-77	RNA polymerase beta subunit
RNA_pol_Rpb2_2	PF04561	208 - 399	9.6e-54	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	472 - 536	1e-24	RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4	PF04566	571 - 632	8.6e-24	RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_5	PF04567	657 - 708	8.2e-18	RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6	PF00562	715 - 1086	9e-142	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7	PF04560	1088 - 1179	8.7e-36	RNA polymerase Rpb2, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011028)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21710
fragaria_vesca	FvH4_3g09270
malus_domestica	MD05G1313100.v1.1 MD10G1295300.v1.1
prunus_persica	Prupe.4G046700_v2.0.a1
pyrus_communis	pycom05g29100 pycom05g29110 pycom10g24710 pycom10g24720
rosa_chinensis	RchiOBHm_Chr5g0008301
rosa_laevigata	RLG00000031560
rosa_multiflora	Rmu_sc0021840.1_g000003
rosa_roxburghii	Rroxscaffold_1G00068160
rosa_rugosa	Rorug04G0434800
rosa_samantha	Rh5AG063600 Rh5BG060100 Rh5CG070400 Rh5CG070500 Rh5DG059600
rosa_wichuraiana	Rw5G005730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	578, 2388
AarI	CACCTGC	1 cut(s)	1405
AasI	GACNNNNNNGTC	1 cut(s)	1872
Acc36I	ACCTGC	1 cut(s)	1405
AccB1I	GGYRCC	1 cut(s)	228
AccB7I	CCANNNNNTGG	1 cut(s)	1067
AccI	GTMKAC	4 cut(s)	177, 1602, 3183, 3268
AccII	CGCG	3 cut(s)	77, 1507, 2677
AccIII	TCCGGA	1 cut(s)	2250
AclWI	GGATC	6 cut(s)	1179, 1305, 1624, 1742, 3187, 3217
AcoI	YGGCCR	2 cut(s)	186, 3513
AcsI	RAATTY	9 cut(s)	514, 813, 1079, 1468, 1640, 1677, 1710, 2822, 3415
AcuI	CTGAAG	7 cut(s)	699, 1578, 1827, 1977, 2034, 2314, 3191
AcvI	CACGTG	1 cut(s)	3203
AcyI	GRCGYC	1 cut(s)	2515
AdeI	CACNNNGTG	1 cut(s)	2690
AfeI	AGCGCT	1 cut(s)	3133
AfiI	CCNNNNNNNGG	6 cut(s)	517, 1067, 1149, 1214, 2813, 3245
AflIII	ACRYGT	2 cut(s)	75, 1063
AgsI	TTSAA	2 cut(s)	643, 2000
AhdI	GACNNNNNGTC	1 cut(s)	2955
AhlI	ACTAGT	1 cut(s)	749
AjnI	CCWGG	5 cut(s)	441, 2391, 2590, 2659, 2871
AjuI	GAANNNNNNNTTGG	2 cut(s)	2231, 2263
AleI	CACNNNNGTG	3 cut(s)	1068, 1276, 3069
Alw21I	GWGCWC	4 cut(s)	15, 623, 876, 3504
AlwI	GGATC	6 cut(s)	1179, 1305, 1624, 1742, 3187, 3217
AlwNI	CAGNNNCTG	1 cut(s)	3169
Ama87I	CYCGRG	3 cut(s)	206, 223, 842
Aor13HI	TCCGGA	1 cut(s)	2250
Aor51HI	AGCGCT	1 cut(s)	3133
AoxI	GGCC	8 cut(s)	81, 186, 203, 710, 1154, 1342, 3148, 3513
ApoI	RAATTY	9 cut(s)	514, 813, 1079, 1468, 1640, 1677, 1710, 2822, 3415
AseI	ATTAAT	1 cut(s)	2049
Asp700I	GAANNNNTTC	2 cut(s)	594, 1056
AspA2I	CCTAGG	1 cut(s)	1144
AspLEI	GCGC	4 cut(s)	123, 2712, 3134, 3523
AspS9I	GGNCC	7 cut(s)	81, 204, 1205, 2428, 2490, 3149, 3212
AsuC2I	CCSGG	3 cut(s)	224, 225, 836
AsuHPI	GGTGA	9 cut(s)	442, 821, 1774, 2227, 2293, 2621, 2800, 2828, 2914
AsuII	TTCGAA	1 cut(s)	1228
AvaI	CYCGRG	3 cut(s)	206, 223, 842
AvaII	GGWCC	4 cut(s)	1205, 2428, 2490, 3212
AvrII	CCTAGG	1 cut(s)	1144
AxyI	CCTNAGG	1 cut(s)	2079
BaeGI	GKGCMC	4 cut(s)	231, 417, 1144, 1544
BalI	TGGCCA	1 cut(s)	3515
BanI	GGYRCC	1 cut(s)	228
BanII	GRGCYC	2 cut(s)	841, 3504
BauI	CACGAG	2 cut(s)	14, 3255
BbrPI	CACGTG	1 cut(s)	3203
BbsI	GAAGAC	2 cut(s)	1468, 2883
Bbv12I	GWGCWC	4 cut(s)	15, 623, 876, 3504
BbvCI	CCTCAGC	1 cut(s)	3243
BceAI	ACGGC	4 cut(s)	68, 1738, 2702, 2869
BciT130I	CCWGG	5 cut(s)	443, 2393, 2592, 2661, 2873
BciVI	GTATCC	1 cut(s)	2251
BclI	TGATCA	1 cut(s)	2734
BcnI	CCSGG	3 cut(s)	224, 225, 836
BcuI	ACTAGT	1 cut(s)	749
BfaI	CTAG	6 cut(s)	492, 750, 869, 968, 1145, 2598
BfoI	RGCGCY	2 cut(s)	3135, 3524
BfuAI	ACCTGC	1 cut(s)	1405
BfuI	GTATCC	1 cut(s)	2251
BglI	GCCNNNNNGGC	1 cut(s)	674
BlnI	CCTAGG	1 cut(s)	1144
BlpI	GCTNAGC	1 cut(s)	3128
Bme1390I	CCNGG	8 cut(s)	224, 225, 443, 836, 2393, 2592, 2661, 2873
Bme18I	GGWCC	4 cut(s)	1205, 2428, 2490, 3212
BmeRI	GACNNNNNGTC	1 cut(s)	2955
BmeT110I	CYCGRG	3 cut(s)	206, 223, 842
BmgT120I	GGNCC	7 cut(s)	81, 204, 1205, 2428, 2490, 3149, 3212
BmiI	GGNNCC	9 cut(s)	230, 474, 1207, 2491, 2492, 2658, 3151, 3213, 3577
BmrFI	CCNGG	8 cut(s)	224, 225, 443, 836, 2393, 2592, 2661, 2873
BmrI	ACTGGG	2 cut(s)	711, 1381
BmsI	GCATC	7 cut(s)	726, 1448, 1951, 2329, 2358, 3004, 3343
BmuI	ACTGGG	2 cut(s)	711, 1381
BoxI	GACNNNNGTC	1 cut(s)	2495
BpiI	GAAGAC	2 cut(s)	1468, 2883
BpmI	CTGGAG	2 cut(s)	1425, 2643
Bpu10I	CCTNAGC	3 cut(s)	1781, 2646, 3243
Bpu1102I	GCTNAGC	1 cut(s)	3128
Bpu14I	TTCGAA	1 cut(s)	1228
BpuEI	CTTGAG	3 cut(s)	591, 1477, 3392
BpuMI	CCSGG	3 cut(s)	224, 225, 836
BsaAI	YACGTR	2 cut(s)	2186, 3203
BsaBI	GATNNNNATC	2 cut(s)	1302, 2172
BsaHI	GRCGYC	1 cut(s)	2515
BsaI	GGTCTC	2 cut(s)	431, 3089
BsaWI	WCCGGW	2 cut(s)	2250, 2442
BsaXI	ACNNNNNCTCC	3 cut(s)	32, 62, 92
Bsc4I	CCNNNNNNNGG	6 cut(s)	517, 1067, 1149, 1214, 2813, 3245
Bse118I	RCCGGY	2 cut(s)	2345, 2723
Bse1I	ACTGG	4 cut(s)	717, 1369, 1376, 3215
Bse21I	CCTNAGG	1 cut(s)	2079
Bse3DI	GCAATG	2 cut(s)	1532, 1934
Bse8I	GATNNNNATC	2 cut(s)	1302, 2172
BseAI	TCCGGA	1 cut(s)	2250
BseBI	CCWGG	5 cut(s)	443, 2393, 2592, 2661, 2873
BseJI	GATNNNNATC	2 cut(s)	1302, 2172
BseLI	CCNNNNNNNGG	6 cut(s)	517, 1067, 1149, 1214, 2813, 3245
BseMI	GCAATG	2 cut(s)	1532, 1934
BseMII	CTCAG	8 cut(s)	381, 426, 525, 2070, 2300, 2660, 3142, 3234
BseNI	ACTGG	4 cut(s)	717, 1369, 1376, 3215
BseRI	GAGGAG	7 cut(s)	20, 23, 119, 2036, 2096, 2489, 2577
BseSI	GKGCMC	4 cut(s)	231, 417, 1144, 1544
BseX3I	CGGCCG	1 cut(s)	186
BseYI	CCCAGC	2 cut(s)	538, 1543
BsgI	GTGCAG	2 cut(s)	3239, 3448
Bsh1236I	CGCG	3 cut(s)	77, 1507, 2677
Bsh1285I	CGRYCG	1 cut(s)	189
BshFI	GGCC	8 cut(s)	83, 188, 205, 712, 1156, 1344, 3150, 3515
BshNI	GGYRCC	1 cut(s)	228
BsiEI	CGRYCG	1 cut(s)	189
BsiHKAI	GWGCWC	4 cut(s)	15, 623, 876, 3504
BsiHKCI	CYCGRG	3 cut(s)	206, 223, 842
BsiSI	CCGG	6 cut(s)	224, 835, 2251, 2346, 2443, 2724
BslFI	GGGAC	6 cut(s)	786, 1191, 2483, 2503, 2863, 3261
BslI	CCNNNNNNNGG	6 cut(s)	517, 1067, 1149, 1214, 2813, 3245
BsmBI	CGTCTC	1 cut(s)	242
BsmFI	GGGAC	6 cut(s)	786, 1191, 2483, 2503, 2863, 3261
BsmI	GAATGC	1 cut(s)	3534
BsnI	GGCC	8 cut(s)	83, 188, 205, 712, 1156, 1344, 3150, 3515
Bso31I	GGTCTC	2 cut(s)	431, 3089
BsoBI	CYCGRG	3 cut(s)	206, 223, 842
Bsp119I	TTCGAA	1 cut(s)	1228
Bsp1286I	GDGCHC	9 cut(s)	15, 231, 417, 623, 841, 876, 1144, 1544, 3504
Bsp13I	TCCGGA	1 cut(s)	2250
Bsp1720I	GCTNAGC	1 cut(s)	3128
Bsp19I	CCATGG	3 cut(s)	2313, 2640, 3307
BspANI	GGCC	8 cut(s)	83, 188, 205, 712, 1156, 1344, 3150, 3515
BspCNI	CTCAG	8 cut(s)	380, 427, 526, 2071, 2299, 2659, 3141, 3235
BspEI	TCCGGA	1 cut(s)	2250
BspFNI	CGCG	3 cut(s)	77, 1507, 2677
BspLI	GGNNCC	9 cut(s)	230, 474, 1207, 2491, 2492, 2658, 3151, 3213, 3577
BspMI	ACCTGC	1 cut(s)	1405
BspPI	GGATC	6 cut(s)	1179, 1305, 1624, 1742, 3187, 3217
BspQI	GCTCTTC	1 cut(s)	3066
BspT104I	TTCGAA	1 cut(s)	1228
BspT107I	GGYRCC	1 cut(s)	228
BspTNI	GGTCTC	2 cut(s)	431, 3089
BsrDI	GCAATG	2 cut(s)	1532, 1934
BsrFI	RCCGGY	2 cut(s)	2345, 2723
BsrI	ACTGG	4 cut(s)	717, 1369, 1376, 3215
BssAI	RCCGGY	2 cut(s)	2345, 2723
BssNAI	GTATAC	1 cut(s)	1603
BssNI	GRCGYC	1 cut(s)	2515
BssSI	CACGAG	2 cut(s)	14, 3255
BssT1I	CCWWGG	5 cut(s)	1144, 2313, 2640, 3145, 3307
Bst1107I	GTATAC	1 cut(s)	1603
Bst2BI	CACGAG	2 cut(s)	14, 3255
Bst2UI	CCWGG	5 cut(s)	443, 2393, 2592, 2661, 2873
Bst6I	CTCTTC	5 cut(s)	641, 798, 1913, 2013, 3066
BstACI	GRCGYC	1 cut(s)	2515
BstAPI	GCANNNNNTGC	1 cut(s)	3435
BstBAI	YACGTR	2 cut(s)	2186, 3203
BstBI	TTCGAA	1 cut(s)	1228
BstDSI	CCRYGG	5 cut(s)	2313, 2431, 2640, 2885, 3307
BstEII	GGTNACC	1 cut(s)	2902
BstFNI	CGCG	3 cut(s)	77, 1507, 2677
BstH2I	RGCGCY	2 cut(s)	3135, 3524
BstHHI	GCGC	4 cut(s)	123, 2712, 3134, 3523
BstMCI	CGRYCG	1 cut(s)	189
BstNI	CCWGG	5 cut(s)	443, 2393, 2592, 2661, 2873
BstNSI	RCATGY	2 cut(s)	2884, 2933
BstPAI	GACNNNNGTC	1 cut(s)	2495
BstPI	GGTNACC	1 cut(s)	2902
BstSCI	CCNGG	8 cut(s)	222, 223, 441, 834, 2391, 2590, 2659, 2871
BstSLI	GKGCMC	4 cut(s)	231, 417, 1144, 1544
BstUI	CGCG	3 cut(s)	77, 1507, 2677
BstV2I	GAAGAC	2 cut(s)	1468, 2883
BstX2I	RGATCY	3 cut(s)	1297, 3192, 3222
BstXI	CCANNNNNNTGG	1 cut(s)	3203
BstYI	RGATCY	3 cut(s)	1297, 3192, 3222
BstZ17I	GTATAC	1 cut(s)	1603
BstZI	CGGCCG	1 cut(s)	186
Bsu36I	CCTNAGG	1 cut(s)	2079
BsuI	GTATCC	1 cut(s)	2251
BsuRI	GGCC	8 cut(s)	83, 188, 205, 712, 1156, 1344, 3150, 3515
BtgI	CCRYGG	5 cut(s)	2313, 2431, 2640, 2885, 3307
BtgZI	GCGATG	1 cut(s)	2658
BtsIMutI	CAGTG	7 cut(s)	432, 1362, 1803, 2010, 2722, 2859, 3222
BveI	ACCTGC	1 cut(s)	1405
CaiI	CAGNNNCTG	1 cut(s)	3169
CfoI	GCGC	4 cut(s)	123, 2712, 3134, 3523
Cfr10I	RCCGGY	2 cut(s)	2345, 2723
Cfr13I	GGNCC	7 cut(s)	81, 204, 1205, 2428, 2490, 3149, 3212
Cfr9I	CCCGGG	1 cut(s)	223
CseI	GACGC	3 cut(s)	83, 1496, 2504
DraIII	CACNNNGTG	1 cut(s)	2690
DrdI	GACNNNNNNGTC	1 cut(s)	1872
DriI	GACNNNNNGTC	1 cut(s)	2955
DseDI	GACNNNNNNGTC	1 cut(s)	1872
EaeI	YGGCCR	2 cut(s)	186, 3513
EagI	CGGCCG	1 cut(s)	186
Eam1104I	CTCTTC	5 cut(s)	641, 798, 1913, 2013, 3066
Eam1105I	GACNNNNNGTC	1 cut(s)	2955
EarI	CTCTTC	5 cut(s)	641, 798, 1913, 2013, 3066
EciI	GGCGGA	2 cut(s)	82, 710
Ecl136II	GAGCTC	1 cut(s)	3502
EclXI	CGGCCG	1 cut(s)	186
Eco130I	CCWWGG	5 cut(s)	1144, 2313, 2640, 3145, 3307
Eco24I	GRGCYC	2 cut(s)	841, 3504
Eco31I	GGTCTC	2 cut(s)	431, 3089
Eco32I	GATATC	2 cut(s)	193, 1828
Eco47I	GGWCC	4 cut(s)	1205, 2428, 2490, 3212
Eco47III	AGCGCT	1 cut(s)	3133
Eco52I	CGGCCG	1 cut(s)	186
Eco53kI	GAGCTC	1 cut(s)	3502
Eco57I	CTGAAG	7 cut(s)	699, 1578, 1827, 1977, 2034, 2314, 3191
Eco72I	CACGTG	1 cut(s)	3203
Eco81I	CCTNAGG	1 cut(s)	2079
Eco88I	CYCGRG	3 cut(s)	206, 223, 842
Eco91I	GGTNACC	1 cut(s)	2902
EcoICRI	GAGCTC	1 cut(s)	3502
EcoO109I	RGGNCCY	1 cut(s)	2490
EcoO65I	GGTNACC	1 cut(s)	2902
EcoRI	GAATTC	3 cut(s)	514, 1468, 3415
EcoRII	CCWGG	5 cut(s)	441, 2391, 2590, 2659, 2871
EcoRV	GATATC	2 cut(s)	193, 1828
EcoT14I	CCWWGG	5 cut(s)	1144, 2313, 2640, 3145, 3307
EcoT22I	ATGCAT	2 cut(s)	1936, 3358
EcoT38I	GRGCYC	2 cut(s)	841, 3504
ErhI	CCWWGG	5 cut(s)	1144, 2313, 2640, 3145, 3307
Esp3I	CGTCTC	1 cut(s)	242
FalI	AAGNNNNNCTT	4 cut(s)	251, 283, 777, 809
FaqI	GGGAC	6 cut(s)	786, 1191, 2483, 2503, 2863, 3261
FauI	CCCGC	3 cut(s)	1565, 2350, 2934
FauNDI	CATATG	2 cut(s)	878, 3177
FbaI	TGATCA	1 cut(s)	2734
FblI	GTMKAC	4 cut(s)	177, 1602, 3183, 3268
FriOI	GRGCYC	2 cut(s)	841, 3504
FspBI	CTAG	6 cut(s)	492, 750, 869, 968, 1145, 2598
GlaI	GCGC	4 cut(s)	122, 2711, 3133, 3522
GsaI	CCCAGC	2 cut(s)	542, 1547
GsuI	CTGGAG	2 cut(s)	1425, 2643
HaeII	RGCGCY	2 cut(s)	3135, 3524
HaeIII	GGCC	8 cut(s)	83, 188, 205, 712, 1156, 1344, 3150, 3515
HapII	CCGG	6 cut(s)	224, 835, 2251, 2346, 2443, 2724
HgaI	GACGC	3 cut(s)	83, 1496, 2504
HhaI	GCGC	4 cut(s)	123, 2712, 3134, 3523
Hin1I	GRCGYC	1 cut(s)	2515
Hin6I	GCGC	4 cut(s)	121, 2710, 3132, 3521
HinP1I	GCGC	4 cut(s)	121, 2710, 3132, 3521
HincII	GTYRAC	6 cut(s)	178, 1248, 1285, 1720, 1801, 3184
HindII	GTYRAC	6 cut(s)	178, 1248, 1285, 1720, 1801, 3184
HindIII	AAGCTT	2 cut(s)	1098, 2704
HpaI	GTTAAC	1 cut(s)	1720
HpaII	CCGG	6 cut(s)	224, 835, 2251, 2346, 2443, 2724
HphI	GGTGA	9 cut(s)	442, 821, 1774, 2227, 2293, 2621, 2800, 2828, 2914
Hpy99I	CGWCG	6 cut(s)	26, 47, 50, 182, 1791, 3188
HpyCH4IV	ACGT	5 cut(s)	362, 1065, 1867, 2185, 3202
HpySE526I	ACGT	5 cut(s)	362, 1065, 1867, 2185, 3202
Hsp92I	GRCGYC	1 cut(s)	2515
HspAI	GCGC	4 cut(s)	121, 2710, 3132, 3521
KflI	GGGWCCC	1 cut(s)	2490
Kpn2I	TCCGGA	1 cut(s)	2250
KroI	GCCGGC	1 cut(s)	2345
KroNI	GCCGGC	1 cut(s)	2347
Ksp22I	TGATCA	1 cut(s)	2734
KspAI	GTTAAC	1 cut(s)	1720
LguI	GCTCTTC	1 cut(s)	3066
LmnI	GCTCC	6 cut(s)	10, 910, 2317, 2590, 2662, 3575
LweI	GCATC	7 cut(s)	726, 1448, 1951, 2329, 2358, 3004, 3343
MaeI	CTAG	6 cut(s)	492, 750, 869, 968, 1145, 2598
MaeII	ACGT	5 cut(s)	362, 1065, 1867, 2185, 3202
MaeIII	GTNAC	8 cut(s)	2233, 2299, 2445, 2678, 2690, 2902, 3034, 3110
MfeI	CAATTG	1 cut(s)	2952
MflI	RGATCY	3 cut(s)	1297, 3192, 3222
MhlI	GDGCHC	9 cut(s)	15, 231, 417, 623, 841, 876, 1144, 1544, 3504
MlsI	TGGCCA	1 cut(s)	3515
MluI	ACGCGT	1 cut(s)	75
MluNI	TGGCCA	1 cut(s)	3515
MlyI	GAGTC	5 cut(s)	128, 191, 218, 707, 2064
MmeI	TCCRAC	2 cut(s)	1054, 1714
Mox20I	TGGCCA	1 cut(s)	3515
Mph1103I	ATGCAT	2 cut(s)	1936, 3358
MroI	TCCGGA	1 cut(s)	2250
MroNI	GCCGGC	1 cut(s)	2345
MroXI	GAANNNNTTC	2 cut(s)	594, 1056
MscI	TGGCCA	1 cut(s)	3515
MseI	TTAA	8 cut(s)	530, 804, 1302, 1421, 1719, 2049, 2778, 3389
MslI	CAYNNNNRTG	7 cut(s)	633, 1068, 1276, 3069, 3201, 3372, 3509
Msp20I	TGGCCA	1 cut(s)	3515
MspA1I	CMGCKG	5 cut(s)	1391, 1513, 1547, 1622, 3242
MspI	CCGG	6 cut(s)	224, 835, 2251, 2346, 2443, 2724
MspR9I	CCNGG	8 cut(s)	224, 225, 443, 836, 2393, 2592, 2661, 2873
MunI	CAATTG	1 cut(s)	2952
Mva1269I	GAATGC	1 cut(s)	3534
MvaI	CCWGG	5 cut(s)	443, 2393, 2592, 2661, 2873
MvnI	CGCG	3 cut(s)	77, 1507, 2677
NaeI	GCCGGC	1 cut(s)	2347
NciI	CCSGG	3 cut(s)	224, 225, 836
NcoI	CCATGG	3 cut(s)	2313, 2640, 3307
NdeI	CATATG	2 cut(s)	878, 3177
NgoMIV	GCCGGC	1 cut(s)	2345
NlaIV	GGNNCC	9 cut(s)	230, 474, 1207, 2491, 2492, 2658, 3151, 3213, 3577
NmeAIII	GCCGAG	1 cut(s)	271
NmuCI	GTSAC	7 cut(s)	2233, 2299, 2445, 2690, 2902, 3034, 3110
NsiI	ATGCAT	2 cut(s)	1936, 3358
NspI	RCATGY	2 cut(s)	2884, 2933
NspV	TTCGAA	1 cut(s)	1228
OliI	CACNNNNGTG	3 cut(s)	1068, 1276, 3069
PaeI	GCATGC	1 cut(s)	2933
PaqCI	CACCTGC	1 cut(s)	1405
PciSI	GCTCTTC	1 cut(s)	3066
PcsI	WCGNNNNNNNCGW	5 cut(s)	21, 81, 138, 186, 1873
PctI	GAATGC	1 cut(s)	3534
PdiI	GCCGGC	1 cut(s)	2347
PdmI	GAANNNNTTC	2 cut(s)	594, 1056
PflFI	GACNNNGTC	1 cut(s)	3210
PflMI	CCANNNNNTGG	1 cut(s)	1067
PfoI	TCCNGGA	1 cut(s)	2590
PleI	GAGTC	5 cut(s)	128, 190, 217, 706, 2064
PmaCI	CACGTG	1 cut(s)	3203
PmlI	CACGTG	1 cut(s)	3203
PpsI	GAGTC	5 cut(s)	128, 190, 217, 706, 2064
Ppu21I	YACGTR	2 cut(s)	2186, 3203
PpuMI	RGGWCCY	1 cut(s)	2490
PshAI	GACNNNNGTC	1 cut(s)	2495
PshBI	ATTAAT	1 cut(s)	2049
PsiI	TTATAA	2 cut(s)	578, 2388
Psp124BI	GAGCTC	1 cut(s)	3504
Psp5II	RGGWCCY	1 cut(s)	2490
Psp6I	CCWGG	5 cut(s)	441, 2391, 2590, 2659, 2871
PspCI	CACGTG	1 cut(s)	3203
PspEI	GGTNACC	1 cut(s)	2902
PspFI	CCCAGC	2 cut(s)	538, 1543
PspGI	CCWGG	5 cut(s)	441, 2391, 2590, 2659, 2871
PspN4I	GGNNCC	9 cut(s)	230, 474, 1207, 2491, 2492, 2658, 3151, 3213, 3577
PspPI	GGNCC	7 cut(s)	81, 204, 1205, 2428, 2490, 3149, 3212
PspPPI	RGGWCCY	1 cut(s)	2490
PstNI	CAGNNNCTG	1 cut(s)	3169
PsuI	RGATCY	3 cut(s)	1297, 3192, 3222
PsyI	GACNNNGTC	1 cut(s)	3210
PvuII	CAGCTG	5 cut(s)	1391, 1513, 1547, 1622, 3242
RseI	CAYNNNNRTG	7 cut(s)	633, 1068, 1276, 3069, 3201, 3372, 3509
SacI	GAGCTC	1 cut(s)	3504
SalI	GTCGAC	2 cut(s)	176, 3182
SapI	GCTCTTC	1 cut(s)	3066
SaqAI	TTAA	8 cut(s)	530, 804, 1302, 1421, 1719, 2049, 2778, 3389
Sau96I	GGNCC	7 cut(s)	81, 204, 1205, 2428, 2490, 3149, 3212
SchI	GAGTC	5 cut(s)	128, 191, 218, 707, 2064
ScrFI	CCNGG	8 cut(s)	224, 225, 443, 836, 2393, 2592, 2661, 2873
SduI	GDGCHC	9 cut(s)	15, 231, 417, 623, 841, 876, 1144, 1544, 3504
SfaNI	GCATC	7 cut(s)	726, 1448, 1951, 2329, 2358, 3004, 3343
SfuI	TTCGAA	1 cut(s)	1228
SinI	GGWCC	4 cut(s)	1205, 2428, 2490, 3212
SmaI	CCCGGG	1 cut(s)	225
SmiMI	CAYNNNNRTG	7 cut(s)	633, 1068, 1276, 3069, 3201, 3372, 3509
SmlI	CTYRAG	3 cut(s)	606, 1456, 3407
SmoI	CTYRAG	3 cut(s)	606, 1456, 3407
SpeI	ACTAGT	1 cut(s)	749
SphI	GCATGC	1 cut(s)	2933
SspI	AATATT	1 cut(s)	1352
SspMI	CTAG	6 cut(s)	492, 750, 869, 968, 1145, 2598
SstI	GAGCTC	1 cut(s)	3504
StyD4I	CCNGG	8 cut(s)	222, 223, 441, 834, 2391, 2590, 2659, 2871
StyI	CCWWGG	5 cut(s)	1144, 2313, 2640, 3145, 3307
TaiI	ACGT	5 cut(s)	365, 1068, 1870, 2188, 3205
TaqI	TCGA	8 cut(s)	102, 132, 141, 177, 195, 1228, 3183, 3509
TaqII	GACCGA	3 cut(s)	317, 477, 675
TatI	WGTACW	2 cut(s)	33, 777
TauI	GCSGC	2 cut(s)	715, 3127
Tru1I	TTAA	8 cut(s)	530, 804, 1302, 1421, 1719, 2049, 2778, 3389
Tru9I	TTAA	8 cut(s)	530, 804, 1302, 1421, 1719, 2049, 2778, 3389
TscAI	CASTG	7 cut(s)	439, 1369, 1810, 2017, 2722, 2859, 3222
TseFI	GTSAC	7 cut(s)	2233, 2299, 2445, 2690, 2902, 3034, 3110
Tsp45I	GTSAC	7 cut(s)	2233, 2299, 2445, 2690, 2902, 3034, 3110
TspDTI	ATGAA	8 cut(s)	66, 72, 139, 451, 1876, 1919, 2426, 3356
TspGWI	ACGGA	3 cut(s)	786, 1165, 1820
TspMI	CCCGGG	1 cut(s)	223
TspRI	CASTG	7 cut(s)	439, 1369, 1810, 2017, 2722, 2859, 3222
Tth111I	GACNNNGTC	1 cut(s)	3210
Van91I	CCANNNNNTGG	1 cut(s)	1067
VpaK11BI	GGWCC	4 cut(s)	1205, 2428, 2490, 3212
VspI	ATTAAT	1 cut(s)	2049
XapI	RAATTY	9 cut(s)	514, 813, 1079, 1468, 1640, 1677, 1710, 2822, 3415
XceI	RCATGY	2 cut(s)	2884, 2933
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2311
XmaI	CCCGGG	1 cut(s)	223
XmaJI	CCTAGG	1 cut(s)	1144
XmiI	GTMKAC	4 cut(s)	177, 1602, 3183, 3268
XmnI	GAANNNNTTC	2 cut(s)	594, 1056
XspI	CTAG	6 cut(s)	492, 750, 869, 968, 1145, 2598
Zsp2I	ATGCAT	2 cut(s)	1936, 3358

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.