Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0011028

Physical Location & Seq

Genomic Coordinates Forward (+)

38286445 .. 38293527

Gene Structure 7083 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1295300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3582 bp

ATGGAGGAGGAGCACGAGTACGACGATCAGCAGTACATGGAGGACGACGACGAAGACGAAATCACGCAGGAGGACGCGTGGGCCGTCATCTCTGCCTACTTCGAGGAGAAGGGCTTGGTGCGCCAGCAGCTCGACTCGTTCGACGAGTTCATTCAGAACACAATGCAGGAGATTGTCGACGAGTCGGCCGATATCGAGATTCGGCCTGAGTCGCAGCACAACCCGGGGCACCAGCCTGATTTCGCCGAGACTGTATGCAAGATTAGCTTTGGTCAGATTTATTTGAGTAAGCCCATGATGACCGAATCTGATGGAGAAACTGCAACCTTATTTCCCAAAGCTGCAAGGCTGAGAAATCTGACGTACTCAGCACCCTTGTATGTGGATGTCTCAAAGAGGATGATAAAGAAAGGACACGATGGCGAAGAACTCACCGAGACCCAGGATTTCACCAAAGTATTCATTGGGAAGGTTCCTATAATGCTTCGGTCTAGTTATTGCACACTGTACCAGAATTCAGAGAAAGATTTAACTGAGCTGGGAGAGTGTCCATACGATCAAGGTGGATATTTTATTATAAATGGGAGTGAAAAGGTTCTTATTGCTCAAGAGAAGATGAGCACCAATCATGTCTATGTGTTCAAGAAGAGGCAGCCGAACAAGTATGCCTATGTGGCTGAAGTTCGGTCTATGGCGGAGTCTCAAAACAGGCCGCCCAGTACCATGTTTGTTCGGATGCTTGCCCGGACTAGTTCTAAAGGGGGTTCTTCGGGACAGTATATCAAAGCTACACTTCCTTATATTAAAGGGGAAATTCCTATTATCATCGTTTTCCGGGCTCTTGGGTTTGTTGCCGATAAAGACATCCTCGAGCACATATGTTATGACTTTTCTGATACTCAAATGATGGAGTTGCTCAGACCTTCTTTGGAAGAAGCATTTGTGATTCAGAATCAACAGGTTGCATTAGATTATATTGGAAAGAGAGGTGCAAATGTTGGTGTAAGTAGAGACAAGAGAATCAAGTACGCGAAAGAGATTCTACAAAAGGAAATGCTTCCACACGTTGGTGTTGGAGAATTTTGTGAGACAAAGAAAGCTTACTATTTTGGATATATCGTTCACAGGCTTCTACTCTGTGCACTTGGTCGGAGGCCAGAGGATGACAGGGATCATTATGGAAACAAGCGGCTTGATCTTGCTGGTCCCTTACTTGGAGGCTTGTTTAGAAATCTATTCAGGAAGTTGACTAAGGATGTGAGAGCTTATGCACAGAAGTGTGTTGACAATGGAAAGGATCTTAATCTGCAATTTGCCATCAAAGCTAAAACCATCACGAGTGGCCTCAAATATTCACTTGCCACTGGAAACTGGGGACAAGCAAATGCAGCTGGTACAAGGGCTGGAGTGTCGCAGGTGTTAAATCGTTTGACATACGCATCCACCCTCTCACACTTGAGAAGACTGAATTCTCCAATAGGGCGTGAAGGGAAATTGGCTAAACCACGTCAGCTGCATAATTCGCAATGGGGAATGATGTGCCCAGCTGAAACACCCGAAGGCCAAGCATGCGGGCTTGTCAAGAATCTTGCGTTGATGGTATACATAACGGTAGGATCAGCTGCATATCCAATCTTGGAATTTTTGGAAGAATGGGGTACAGAAAACTTTGAGGAAATTTCACCTGCGGTTATACCCCAAGCTACAAAAATTTTTGTTAACGGCTGTTGGGTTGGAATCCATCGTGATCCTGAAATGTTGGTGAGAACATTAAGAAAGCTAAGGCGACGGGTTGATGTCAACACTGAAGTTGGGGTTGTGAGGGATATCCGTCTGAAAGAATTGCGGATATATACTGACTATGGCCGTTGCAGTCGTCCATTATTCATTGTGGACAAACAGAGGCTTCTCATAAAGAAGCGAGATATTCATGCATTGCAGCAGAGGGAAAGCCCTGAAGATGCTGGTTGGCATGACCTTGTAGCGAAGGGATATATTGAATATATTGACACTGAAGAAGAGGAGACTACAATGATTTCCATGACAATTAATGATCTTGTCCAAGCAAGACTCAATCCTGAGGAGGCATATTCGGACACTTATACACATTGTGAAATCCACCCATCACTTATTTTGGGTGTTTGTGCTTCTATTATACCATTTCCTGATCATAATCAGTCTCCACGTAATACGTATCAATCTGCTATGGGGAAGCAAGCTATGGGGATTTATGTCACCAATTATCAGTTCCGGATGGATACATTGGCGTATGTTTTATACTATCCTCAGAAACCACTTGTCACCACACGAGCCATGGAGCATCTCCACTTCCGGCAGCTTCCCGCTGGCATCAATGCTATTGTTGCCATTTCCTGTTATTCCGGTTATAACCAGGAAGATTCTGTCATTATGAATCAATCCTCTGTGGACCGTGGATTCTTCCGGTCACTATTCTTTCGATCTTATAGGGATGAGGAGAAAAAGATGGGAACCCTTGTCAAAGAGGATTTTGGGCGTCCTGACAGAGCAAATACTATGGGTATGCGACATGGTTCTTATGACAAATTGGATGATGATGGTCTTGCTCCTCCTGGAACTAGGGTTTCAGGTGAGGATGTCATCATAGGAAAGACCACACCCATGGCTCAGGACGAGGCTCCAGGGCAAGCGACATCGCGTTACACGCGGCGTGACCACAGCATAAGCTTGCGCCACAGTGAAACCGGCATAGTTGATCAGGTTCTACTGACGACCAATGCCGATGGGTTGCGGTTTGTCAAAGTAAGGGTGAGGTCAGTCAGAATACCGCAGATTGGTGACAAATTCAGTAGCAGACATGGACAGAAGGGGACAGTGGGCATGACTTATACGCAGGAAGACATGCCATGGACTGTGGAAGGGGTCACACCTGATATAATCGTGAACCCACACGCTATTCCGTCTCGTATGACAATTGGTCAGCTGATTGAGTGTATCATGGGGAAGGTTGCAGCTCACATGGGAAAGGAAGGAGATGCTACTCCTTTTACAGATGTGACGGTGGACAATATCAGTAAAGCTCTTCACAGTTGTGGTTATCAAATGCGTGGATTCGAGACCATGTATAATGGTCACACGGGCAGGCGGCTCAGCGCTATGATATTCCTTGGCCCCACATATTACCAAAGACTGAAGCACATGGTCGACGACAAGATCCATTCACGTGGACGGGGCCCAGTGCAGATCCTCACAAGGCAGCCAGCTGAGGGACGGTCTCGTGATGGTGGTCTACGATTTGGAGAAATGGAACGAGATTGCATGATTGCCCATGGTGCTGCCCACTTTCTGAAAGAAAGATTGTTCGACCAGAGTGATGCATATAGGGTTCATGTCTGCGAGCGTTGTGGGTTAATAGCTATTGCAAATCTCAAGAAGAATTCGTTTGAGTGCAGGGGTTGCAAGAACAAAACTGACATTGTTCAGGTTTACATTCCTTATGCCTGCAAGCTGCTATTCCAAGAGCTCATGTCAATGGCCATAGCGCCAAGAATGCTCACAAAGGACATAAAGCCGGCCAAAAACCAAAAGAAGAAAGGAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005665 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016591 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030054 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1194

Amino Acids

135.23

Weight (kDa)

7.73

Isoelectric Point (pI)

41.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	38 - 447	3.2e-77	RNA polymerase beta subunit
RNA_pol_Rpb2_2	PF04561	208 - 399	9.6e-54	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	472 - 536	1e-24	RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4	PF04566	571 - 632	8.6e-24	RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_5	PF04567	657 - 708	8.2e-18	RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6	PF00562	715 - 1086	9e-142	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7	PF04560	1088 - 1179	8.7e-36	RNA polymerase Rpb2, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011028)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21710
fragaria_vesca	FvH4_3g09270
malus_domestica	MD05G1313100.v1.1 MD10G1295300.v1.1
prunus_persica	Prupe.4G046700_v2.0.a1
pyrus_communis	pycom05g29100 pycom05g29110 pycom10g24710 pycom10g24720
rosa_chinensis	RchiOBHm_Chr5g0008301
rosa_laevigata	RLG00000031560
rosa_multiflora	Rmu_sc0021840.1_g000003
rosa_roxburghii	Rroxscaffold_1G00068160
rosa_rugosa	Rorug04G0434800
rosa_samantha	Rh5AG063600 Rh5BG060100 Rh5CG070400 Rh5CG070500 Rh5DG059600
rosa_wichuraiana	Rw5G005730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	578, 2388
AarI	CACCTGC	2 cut(s)	1405, 1693
Acc36I	ACCTGC	2 cut(s)	1405, 1693
AccB1I	GGYRCC	1 cut(s)	228
AccB7I	CCANNNNNTGG	1 cut(s)	1067
AccI	GTMKAC	4 cut(s)	177, 1602, 3183, 3268
AccII	CGCG	4 cut(s)	77, 1031, 2677, 2686
AccIII	TCCGGA	1 cut(s)	2250
AclWI	GGATC	6 cut(s)	1179, 1305, 1624, 1742, 3187, 3217
AcoI	YGGCCR	4 cut(s)	186, 1864, 3513, 3552
AcsI	RAATTY	9 cut(s)	514, 813, 1079, 1468, 1640, 1677, 1710, 2822, 3415
AcuI	CTGAAG	5 cut(s)	699, 1827, 1977, 2034, 3191
AcvI	CACGTG	1 cut(s)	3203
AcyI	GRCGYC	1 cut(s)	2515
AdeI	CACNNNGTG	1 cut(s)	2111
AfaI	GTAC	8 cut(s)	20, 35, 365, 509, 721, 1028, 1396, 1660
AfeI	AGCGCT	1 cut(s)	3133
AfiI	CCNNNNNNNGG	4 cut(s)	1067, 1214, 2813, 3245
AflIII	ACRYGT	2 cut(s)	75, 1063
AgsI	TTSAA	2 cut(s)	643, 2000
AhdI	GACNNNNNGTC	1 cut(s)	2955
AhlI	ACTAGT	1 cut(s)	749
AjiI	CACGTC	1 cut(s)	1508
AjnI	CCWGG	4 cut(s)	441, 2391, 2590, 2659
AjuI	GAANNNNNNNTTGG	2 cut(s)	2231, 2263
AleI	CACNNNNGTG	3 cut(s)	1068, 1276, 3069
AloI	GAACNNNNNNTCC	2 cut(s)	1104, 1136
Alw21I	GWGCWC	5 cut(s)	15, 623, 876, 1144, 3504
Alw44I	GTGCAC	1 cut(s)	1140
AlwI	GGATC	6 cut(s)	1179, 1305, 1624, 1742, 3187, 3217
Ama87I	CYCGRG	2 cut(s)	223, 869
Aor13HI	TCCGGA	1 cut(s)	2250
Aor51HI	AGCGCT	1 cut(s)	3133
ApaI	GGGCCC	1 cut(s)	3215
ApaLI	GTGCAC	1 cut(s)	1140
ApoI	RAATTY	9 cut(s)	514, 813, 1079, 1468, 1640, 1677, 1710, 2822, 3415
AseI	ATTAAT	1 cut(s)	2049
Asp700I	GAANNNNTTC	2 cut(s)	594, 1056
AspLEI	GCGC	4 cut(s)	123, 2712, 3134, 3523
AspS9I	GGNCC	6 cut(s)	81, 1205, 2428, 3149, 3211, 3212
AsuC2I	CCSGG	4 cut(s)	224, 225, 745, 836
AsuHPI	GGTGA	9 cut(s)	424, 442, 1674, 1774, 2227, 2293, 2621, 2800, 2828
AvaI	CYCGRG	2 cut(s)	223, 869
AvaII	GGWCC	2 cut(s)	1205, 2428
AxyI	CCTNAGG	1 cut(s)	2079
BaeGI	GKGCMC	4 cut(s)	231, 1144, 1544, 3215
BalI	TGGCCA	1 cut(s)	3515
BanI	GGYRCC	1 cut(s)	228
BanII	GRGCYC	3 cut(s)	841, 3215, 3504
BauI	CACGAG	4 cut(s)	14, 1336, 2307, 3255
BbrPI	CACGTG	1 cut(s)	3203
BbsI	GAAGAC	3 cut(s)	60, 1468, 2883
Bbv12I	GWGCWC	5 cut(s)	15, 623, 876, 1144, 3504
BbvCI	CCTCAGC	1 cut(s)	3243
BceAI	ACGGC	3 cut(s)	68, 1738, 1851
BciT130I	CCWGG	4 cut(s)	443, 2393, 2592, 2661
BciVI	GTATCC	1 cut(s)	2251
BclI	TGATCA	2 cut(s)	2167, 2734
BcnI	CCSGG	4 cut(s)	224, 225, 745, 836
BcuI	ACTAGT	1 cut(s)	749
BfaI	CTAG	3 cut(s)	492, 750, 2598
BfoI	RGCGCY	2 cut(s)	3135, 3524
BfuAI	ACCTGC	2 cut(s)	1405, 1693
BfuI	GTATCC	1 cut(s)	2251
BglI	GCCNNNNNGGC	1 cut(s)	674
BlpI	GCTNAGC	1 cut(s)	3128
Bme1390I	CCNGG	8 cut(s)	224, 225, 443, 745, 836, 2393, 2592, 2661
Bme18I	GGWCC	2 cut(s)	1205, 2428
BmeRI	GACNNNNNGTC	1 cut(s)	2955
BmeT110I	CYCGRG	2 cut(s)	223, 869
BmgBI	CACGTC	1 cut(s)	1508
BmgT120I	GGNCC	6 cut(s)	81, 1205, 2428, 3149, 3211, 3212
BmiI	GGNNCC	8 cut(s)	230, 474, 1207, 2491, 2658, 3151, 3212, 3213
BmrFI	CCNGG	8 cut(s)	224, 225, 443, 745, 836, 2393, 2592, 2661
BmrI	ACTGGG	3 cut(s)	711, 1381, 3209
BmsI	GCATC	7 cut(s)	726, 1448, 1951, 2329, 2358, 3004, 3343
BmuI	ACTGGG	3 cut(s)	711, 1381, 3209
BpiI	GAAGAC	3 cut(s)	60, 1468, 2883
BpmI	CTGGAG	2 cut(s)	1425, 2643
Bpu10I	CCTNAGC	3 cut(s)	1781, 2646, 3243
Bpu1102I	GCTNAGC	1 cut(s)	3128
BpuEI	CTTGAG	3 cut(s)	591, 1477, 3392
BpuMI	CCSGG	4 cut(s)	224, 225, 745, 836
BsaAI	YACGTR	3 cut(s)	2186, 2193, 3203
BsaBI	GATNNNNATC	2 cut(s)	1302, 2172
BsaHI	GRCGYC	1 cut(s)	2515
BsaI	GGTCTC	3 cut(s)	431, 3089, 3258
BsaWI	WCCGGW	3 cut(s)	2250, 2381, 2442
BsaXI	ACNNNNNCTCC	3 cut(s)	32, 62, 92
Bsc4I	CCNNNNNNNGG	4 cut(s)	1067, 1214, 2813, 3245
Bse118I	RCCGGY	2 cut(s)	2723, 3550
Bse1I	ACTGG	4 cut(s)	717, 1369, 1376, 3215
Bse21I	CCTNAGG	1 cut(s)	2079
Bse3DI	GCAATG	2 cut(s)	1532, 1934
Bse8I	GATNNNNATC	2 cut(s)	1302, 2172
BseAI	TCCGGA	1 cut(s)	2250
BseBI	CCWGG	4 cut(s)	443, 2393, 2592, 2661
BseJI	GATNNNNATC	2 cut(s)	1302, 2172
BseLI	CCNNNNNNNGG	4 cut(s)	1067, 1214, 2813, 3245
BseMI	GCAATG	2 cut(s)	1532, 1934
BseNI	ACTGG	4 cut(s)	717, 1369, 1376, 3215
BseRI	GAGGAG	7 cut(s)	20, 23, 119, 2036, 2096, 2489, 2577
BseSI	GKGCMC	4 cut(s)	231, 1144, 1544, 3215
BseX3I	CGGCCG	1 cut(s)	186
BseYI	CCCAGC	2 cut(s)	538, 1543
BsgI	GTGCAG	2 cut(s)	3239, 3448
Bsh1236I	CGCG	4 cut(s)	77, 1031, 2677, 2686
Bsh1285I	CGRYCG	1 cut(s)	189
BshNI	GGYRCC	1 cut(s)	228
BsiEI	CGRYCG	1 cut(s)	189
BsiHKAI	GWGCWC	5 cut(s)	15, 623, 876, 1144, 3504
BsiHKCI	CYCGRG	2 cut(s)	223, 869
BsiSI	CCGG	9 cut(s)	224, 745, 835, 2251, 2332, 2382, 2443, 2724, 3551
BslFI	GGGAC	5 cut(s)	786, 1191, 1389, 2863, 3261
BslI	CCNNNNNNNGG	4 cut(s)	1067, 1214, 2813, 3245
BsmBI	CGTCTC	1 cut(s)	2946
BsmFI	GGGAC	5 cut(s)	786, 1191, 1389, 2863, 3261
BsmI	GAATGC	1 cut(s)	3534
Bso31I	GGTCTC	3 cut(s)	431, 3089, 3258
BsoBI	CYCGRG	2 cut(s)	223, 869
Bsp120I	GGGCCC	1 cut(s)	3211
Bsp1286I	GDGCHC	9 cut(s)	15, 231, 623, 841, 876, 1144, 1544, 3215, 3504
Bsp13I	TCCGGA	1 cut(s)	2250
Bsp1720I	GCTNAGC	1 cut(s)	3128
Bsp19I	CCATGG	4 cut(s)	2313, 2640, 2885, 3307
BspEI	TCCGGA	1 cut(s)	2250
BspFNI	CGCG	4 cut(s)	77, 1031, 2677, 2686
BspLI	GGNNCC	8 cut(s)	230, 474, 1207, 2491, 2658, 3151, 3212, 3213
BspMI	ACCTGC	2 cut(s)	1405, 1693
BspPI	GGATC	6 cut(s)	1179, 1305, 1624, 1742, 3187, 3217
BspQI	GCTCTTC	1 cut(s)	3066
BspT107I	GGYRCC	1 cut(s)	228
BspTNI	GGTCTC	3 cut(s)	431, 3089, 3258
BsrDI	GCAATG	2 cut(s)	1532, 1934
BsrFI	RCCGGY	2 cut(s)	2723, 3550
BsrI	ACTGG	4 cut(s)	717, 1369, 1376, 3215
BssAI	RCCGGY	2 cut(s)	2723, 3550
BssNAI	GTATAC	1 cut(s)	1603
BssNI	GRCGYC	1 cut(s)	2515
BssSI	CACGAG	4 cut(s)	14, 1336, 2307, 3255
BssT1I	CCWWGG	5 cut(s)	2313, 2640, 2885, 3145, 3307
Bst1107I	GTATAC	1 cut(s)	1603
Bst2BI	CACGAG	4 cut(s)	14, 1336, 2307, 3255
Bst2UI	CCWGG	4 cut(s)	443, 2393, 2592, 2661
Bst6I	CTCTTC	3 cut(s)	641, 2013, 3066
BstACI	GRCGYC	1 cut(s)	2515
BstAPI	GCANNNNNTGC	1 cut(s)	3435
BstBAI	YACGTR	3 cut(s)	2186, 2193, 3203
BstDSI	CCRYGG	5 cut(s)	2313, 2431, 2640, 2885, 3307
BstFNI	CGCG	4 cut(s)	77, 1031, 2677, 2686
BstH2I	RGCGCY	2 cut(s)	3135, 3524
BstHHI	GCGC	4 cut(s)	123, 2712, 3134, 3523
BstMCI	CGRYCG	1 cut(s)	189
BstNI	CCWGG	4 cut(s)	443, 2393, 2592, 2661
BstNSI	RCATGY	2 cut(s)	1572, 2884
BstSCI	CCNGG	8 cut(s)	222, 223, 441, 743, 834, 2391, 2590, 2659
BstSLI	GKGCMC	4 cut(s)	231, 1144, 1544, 3215
BstSNI	TACGTA	1 cut(s)	2193
BstUI	CGCG	4 cut(s)	77, 1031, 2677, 2686
BstV2I	GAAGAC	3 cut(s)	60, 1468, 2883
BstX2I	RGATCY	3 cut(s)	1297, 3192, 3222
BstXI	CCANNNNNNTGG	2 cut(s)	2641, 3203
BstYI	RGATCY	3 cut(s)	1297, 3192, 3222
BstZ17I	GTATAC	1 cut(s)	1603
BstZI	CGGCCG	1 cut(s)	186
Bsu36I	CCTNAGG	1 cut(s)	2079
BsuI	GTATCC	1 cut(s)	2251
BtgI	CCRYGG	5 cut(s)	2313, 2431, 2640, 2885, 3307
BtgZI	GCGATG	1 cut(s)	2658
BtrI	CACGTC	1 cut(s)	1508
BtsIMutI	CAGTG	7 cut(s)	503, 1362, 1803, 2010, 2722, 2859, 3222
BveI	ACCTGC	2 cut(s)	1405, 1693
CfoI	GCGC	4 cut(s)	123, 2712, 3134, 3523
Cfr10I	RCCGGY	2 cut(s)	2723, 3550
Cfr13I	GGNCC	6 cut(s)	81, 1205, 2428, 3149, 3211, 3212
Cfr9I	CCCGGG	1 cut(s)	223
CseI	GACGC	2 cut(s)	83, 2504
Csp6I	GTAC	8 cut(s)	19, 34, 364, 508, 720, 1027, 1395, 1659
CviQI	GTAC	8 cut(s)	19, 34, 364, 508, 720, 1027, 1395, 1659
DraIII	CACNNNGTG	1 cut(s)	2111
DriI	GACNNNNNGTC	1 cut(s)	2955
EaeI	YGGCCR	4 cut(s)	186, 1864, 3513, 3552
EagI	CGGCCG	1 cut(s)	186
Eam1104I	CTCTTC	3 cut(s)	641, 2013, 3066
Eam1105I	GACNNNNNGTC	1 cut(s)	2955
EarI	CTCTTC	3 cut(s)	641, 2013, 3066
EciI	GGCGGA	1 cut(s)	710
Ecl136II	GAGCTC	1 cut(s)	3502
EclXI	CGGCCG	1 cut(s)	186
Eco105I	TACGTA	1 cut(s)	2193
Eco130I	CCWWGG	5 cut(s)	2313, 2640, 2885, 3145, 3307
Eco24I	GRGCYC	3 cut(s)	841, 3215, 3504
Eco31I	GGTCTC	3 cut(s)	431, 3089, 3258
Eco32I	GATATC	2 cut(s)	193, 1828
Eco47I	GGWCC	2 cut(s)	1205, 2428
Eco47III	AGCGCT	1 cut(s)	3133
Eco52I	CGGCCG	1 cut(s)	186
Eco53kI	GAGCTC	1 cut(s)	3502
Eco57I	CTGAAG	5 cut(s)	699, 1827, 1977, 2034, 3191
Eco72I	CACGTG	1 cut(s)	3203
Eco81I	CCTNAGG	1 cut(s)	2079
Eco88I	CYCGRG	2 cut(s)	223, 869
EcoICRI	GAGCTC	1 cut(s)	3502
EcoO109I	RGGNCCY	1 cut(s)	3211
EcoRI	GAATTC	3 cut(s)	514, 1468, 3415
EcoRII	CCWGG	4 cut(s)	441, 2391, 2590, 2659
EcoRV	GATATC	2 cut(s)	193, 1828
EcoT14I	CCWWGG	5 cut(s)	2313, 2640, 2885, 3145, 3307
EcoT22I	ATGCAT	2 cut(s)	1936, 3358
EcoT38I	GRGCYC	3 cut(s)	841, 3215, 3504
ErhI	CCWWGG	5 cut(s)	2313, 2640, 2885, 3145, 3307
Esp3I	CGTCTC	1 cut(s)	2946
FalI	AAGNNNNNCTT	4 cut(s)	251, 283, 777, 809
FaqI	GGGAC	5 cut(s)	786, 1191, 1389, 2863, 3261
FauI	CCCGC	2 cut(s)	1565, 2350
FauNDI	CATATG	1 cut(s)	878
FbaI	TGATCA	2 cut(s)	2167, 2734
FblI	GTMKAC	4 cut(s)	177, 1602, 3183, 3268
FriOI	GRGCYC	3 cut(s)	841, 3215, 3504
FspBI	CTAG	3 cut(s)	492, 750, 2598
GlaI	GCGC	4 cut(s)	122, 2711, 3133, 3522
GsaI	CCCAGC	2 cut(s)	542, 1547
GsuI	CTGGAG	2 cut(s)	1425, 2643
HaeII	RGCGCY	2 cut(s)	3135, 3524
HapII	CCGG	9 cut(s)	224, 745, 835, 2251, 2332, 2382, 2443, 2724, 3551
HgaI	GACGC	2 cut(s)	83, 2504
HhaI	GCGC	4 cut(s)	123, 2712, 3134, 3523
Hin1I	GRCGYC	1 cut(s)	2515
Hin6I	GCGC	4 cut(s)	121, 2710, 3132, 3521
HinP1I	GCGC	4 cut(s)	121, 2710, 3132, 3521
HincII	GTYRAC	6 cut(s)	178, 1248, 1285, 1720, 1801, 3184
HindII	GTYRAC	6 cut(s)	178, 1248, 1285, 1720, 1801, 3184
HindIII	AAGCTT	2 cut(s)	1098, 2704
HpaI	GTTAAC	1 cut(s)	1720
HpaII	CCGG	9 cut(s)	224, 745, 835, 2251, 2332, 2382, 2443, 2724, 3551
HphI	GGTGA	9 cut(s)	424, 442, 1674, 1774, 2227, 2293, 2621, 2800, 2828
Hpy99I	CGWCG	7 cut(s)	26, 50, 53, 146, 182, 1791, 3188
HpyCH4IV	ACGT	6 cut(s)	362, 1065, 1507, 2185, 2192, 3202
HpySE526I	ACGT	6 cut(s)	362, 1065, 1507, 2185, 2192, 3202
Hsp92I	GRCGYC	1 cut(s)	2515
HspAI	GCGC	4 cut(s)	121, 2710, 3132, 3521
Kpn2I	TCCGGA	1 cut(s)	2250
KroI	GCCGGC	1 cut(s)	3550
KroNI	GCCGGC	1 cut(s)	3552
Ksp22I	TGATCA	2 cut(s)	2167, 2734
KspAI	GTTAAC	1 cut(s)	1720
LguI	GCTCTTC	1 cut(s)	3066
LmnI	GCTCC	4 cut(s)	10, 2317, 2590, 2662
LweI	GCATC	7 cut(s)	726, 1448, 1951, 2329, 2358, 3004, 3343
MaeI	CTAG	3 cut(s)	492, 750, 2598
MaeII	ACGT	6 cut(s)	362, 1065, 1507, 2185, 2192, 3202
MaeIII	GTNAC	9 cut(s)	2233, 2299, 2445, 2678, 2690, 2816, 2902, 3034, 3110
MfeI	CAATTG	1 cut(s)	2952
MflI	RGATCY	3 cut(s)	1297, 3192, 3222
MhlI	GDGCHC	9 cut(s)	15, 231, 623, 841, 876, 1144, 1544, 3215, 3504
MlsI	TGGCCA	1 cut(s)	3515
MluI	ACGCGT	1 cut(s)	75
MluNI	TGGCCA	1 cut(s)	3515
MlyI	GAGTC	5 cut(s)	128, 191, 218, 707, 2064
MmeI	TCCRAC	3 cut(s)	1054, 1130, 1714
Mox20I	TGGCCA	1 cut(s)	3515
Mph1103I	ATGCAT	2 cut(s)	1936, 3358
MroI	TCCGGA	1 cut(s)	2250
MroNI	GCCGGC	1 cut(s)	3550
MroXI	GAANNNNTTC	2 cut(s)	594, 1056
MscI	TGGCCA	1 cut(s)	3515
MseI	TTAA	8 cut(s)	530, 804, 1302, 1421, 1719, 1772, 2049, 3389
MslI	CAYNNNNRTG	7 cut(s)	633, 1068, 1276, 2639, 3069, 3201, 3509
Msp20I	TGGCCA	1 cut(s)	3515
MspA1I	CMGCKG	7 cut(s)	1391, 1513, 1547, 1622, 2345, 2962, 3242
MspI	CCGG	9 cut(s)	224, 745, 835, 2251, 2332, 2382, 2443, 2724, 3551
MspR9I	CCNGG	8 cut(s)	224, 225, 443, 745, 836, 2393, 2592, 2661
MunI	CAATTG	1 cut(s)	2952
Mva1269I	GAATGC	1 cut(s)	3534
MvaI	CCWGG	4 cut(s)	443, 2393, 2592, 2661
MvnI	CGCG	4 cut(s)	77, 1031, 2677, 2686
NaeI	GCCGGC	1 cut(s)	3552
NciI	CCSGG	4 cut(s)	224, 225, 745, 836
NcoI	CCATGG	4 cut(s)	2313, 2640, 2885, 3307
NdeI	CATATG	1 cut(s)	878
NgoMIV	GCCGGC	1 cut(s)	3550
NlaIV	GGNNCC	8 cut(s)	230, 474, 1207, 2491, 2658, 3151, 3212, 3213
NmeAIII	GCCGAG	1 cut(s)	271
NmuCI	GTSAC	8 cut(s)	2233, 2299, 2445, 2690, 2816, 2902, 3034, 3110
NsiI	ATGCAT	2 cut(s)	1936, 3358
NspI	RCATGY	2 cut(s)	1572, 2884
OliI	CACNNNNGTG	3 cut(s)	1068, 1276, 3069
PaeI	GCATGC	1 cut(s)	1572
PaeR7I	CTCGAG	1 cut(s)	869
PaqCI	CACCTGC	2 cut(s)	1405, 1693
PciSI	GCTCTTC	1 cut(s)	3066
PcsI	WCGNNNNNNNCGW	5 cut(s)	21, 54, 81, 138, 186
PctI	GAATGC	1 cut(s)	3534
PdiI	GCCGGC	1 cut(s)	3552
PdmI	GAANNNNTTC	2 cut(s)	594, 1056
PflMI	CCANNNNNTGG	1 cut(s)	1067
PfoI	TCCNGGA	1 cut(s)	2590
PleI	GAGTC	5 cut(s)	128, 190, 217, 706, 2064
PmaCI	CACGTG	1 cut(s)	3203
PmlI	CACGTG	1 cut(s)	3203
PpsI	GAGTC	5 cut(s)	128, 190, 217, 706, 2064
Ppu21I	YACGTR	3 cut(s)	2186, 2193, 3203
PshBI	ATTAAT	1 cut(s)	2049
PsiI	TTATAA	2 cut(s)	578, 2388
Psp124BI	GAGCTC	1 cut(s)	3504
Psp6I	CCWGG	4 cut(s)	441, 2391, 2590, 2659
PspCI	CACGTG	1 cut(s)	3203
PspFI	CCCAGC	2 cut(s)	538, 1543
PspGI	CCWGG	4 cut(s)	441, 2391, 2590, 2659
PspN4I	GGNNCC	8 cut(s)	230, 474, 1207, 2491, 2658, 3151, 3212, 3213
PspOMI	GGGCCC	1 cut(s)	3211
PspPI	GGNCC	6 cut(s)	81, 1205, 2428, 3149, 3211, 3212
PspXI	VCTCGAGB	1 cut(s)	869
PsuI	RGATCY	3 cut(s)	1297, 3192, 3222
PvuII	CAGCTG	6 cut(s)	1391, 1513, 1547, 1622, 2962, 3242
RsaI	GTAC	8 cut(s)	20, 35, 365, 509, 721, 1028, 1396, 1660
RsaNI	GTAC	8 cut(s)	19, 34, 364, 508, 720, 1027, 1395, 1659
RseI	CAYNNNNRTG	7 cut(s)	633, 1068, 1276, 2639, 3069, 3201, 3509
SacI	GAGCTC	1 cut(s)	3504
SalI	GTCGAC	2 cut(s)	176, 3182
SapI	GCTCTTC	1 cut(s)	3066
SaqAI	TTAA	8 cut(s)	530, 804, 1302, 1421, 1719, 1772, 2049, 3389
Sau96I	GGNCC	6 cut(s)	81, 1205, 2428, 3149, 3211, 3212
SchI	GAGTC	5 cut(s)	128, 191, 218, 707, 2064
ScrFI	CCNGG	8 cut(s)	224, 225, 443, 745, 836, 2393, 2592, 2661
SduI	GDGCHC	9 cut(s)	15, 231, 623, 841, 876, 1144, 1544, 3215, 3504
SfaNI	GCATC	7 cut(s)	726, 1448, 1951, 2329, 2358, 3004, 3343
Sfr274I	CTCGAG	1 cut(s)	869
SinI	GGWCC	2 cut(s)	1205, 2428
SlaI	CTCGAG	1 cut(s)	869
SmaI	CCCGGG	1 cut(s)	225
SmiMI	CAYNNNNRTG	7 cut(s)	633, 1068, 1276, 2639, 3069, 3201, 3509
SmlI	CTYRAG	4 cut(s)	606, 869, 1456, 3407
SmoI	CTYRAG	4 cut(s)	606, 869, 1456, 3407
SnaBI	TACGTA	1 cut(s)	2193
SpeI	ACTAGT	1 cut(s)	749
SphI	GCATGC	1 cut(s)	1572
SspI	AATATT	1 cut(s)	1352
SspMI	CTAG	3 cut(s)	492, 750, 2598
SstI	GAGCTC	1 cut(s)	3504
StyD4I	CCNGG	8 cut(s)	222, 223, 441, 743, 834, 2391, 2590, 2659
StyI	CCWWGG	5 cut(s)	2313, 2640, 2885, 3145, 3307
TaiI	ACGT	6 cut(s)	365, 1068, 1510, 2188, 2195, 3205
TaqII	GACCGA	3 cut(s)	317, 477, 675
TatI	WGTACW	1 cut(s)	33
TauI	GCSGC	4 cut(s)	715, 1192, 2689, 3127
Tru1I	TTAA	8 cut(s)	530, 804, 1302, 1421, 1719, 1772, 2049, 3389
Tru9I	TTAA	8 cut(s)	530, 804, 1302, 1421, 1719, 1772, 2049, 3389
TscAI	CASTG	7 cut(s)	510, 1369, 1810, 2017, 2722, 2859, 3222
TseFI	GTSAC	8 cut(s)	2233, 2299, 2445, 2690, 2816, 2902, 3034, 3110
Tsp45I	GTSAC	8 cut(s)	2233, 2299, 2445, 2690, 2816, 2902, 3034, 3110
TspDTI	ATGAA	6 cut(s)	139, 451, 1876, 1919, 2426, 3356
TspGWI	ACGGA	2 cut(s)	1820, 2928
TspMI	CCCGGG	1 cut(s)	223
TspRI	CASTG	7 cut(s)	510, 1369, 1810, 2017, 2722, 2859, 3222
Van91I	CCANNNNNTGG	1 cut(s)	1067
VneI	GTGCAC	1 cut(s)	1140
VpaK11BI	GGWCC	2 cut(s)	1205, 2428
VspI	ATTAAT	1 cut(s)	2049
XapI	RAATTY	9 cut(s)	514, 813, 1079, 1468, 1640, 1677, 1710, 2822, 3415
XceI	RCATGY	2 cut(s)	1572, 2884
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2311
XhoI	CTCGAG	1 cut(s)	869
XmaI	CCCGGG	1 cut(s)	223
XmiI	GTMKAC	4 cut(s)	177, 1602, 3183, 3268
XmnI	GAANNNNTTC	2 cut(s)	594, 1056
XspI	CTAG	3 cut(s)	492, 750, 2598
Zsp2I	ATGCAT	2 cut(s)	1936, 3358

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.