Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0011028

Physical Location & Seq

Genomic Coordinates Reverse (-)

5348209 .. 5355654

Gene Structure 7446 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG063600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3072 bp

ATGGAAGAAGAGCCGGAGTACGAAGAGCAGTACAACGAGGAGGACGACGAAGATGAGATAACGCAGGAAGACGCGTGGGCTGTGATTTCAGCCTATTTCGAAGAGAAGGGTTTAGTGAGGCAGCAGCTGGACTCGTTCGATGAGTTCATTCAGAACACAATGCAGGAGATTGTCGATGAGGCCGCCGATATCGAAATCCGACCCGAATCGCAGCATAACCCGGGTCACCAACCCGATTTCGCTGAGACTATATACAAAATCAGCTTCGGTCAGATTTATCTGAGTAAGCCTATGATGACCGAGTCGGATGGTGAAACTGCAACGCTGTTTCCGAAAGCTGCGAGGTTGCGAAACTTGACCTACTCGGCGCCTTTGTATGTGGATGTGACCAAGAGGGTTATAAAGAAAGGACATGATGGTGAAGAAGTTACTGAGACTCAGGATTTCACCAAAGTGTTCATTGGAAAGGTCCCAATTATGCTCCGTTCGAGTTATTGTACATTGTATCAGAATTCGGAGAAAGACTTGACGGAGCTGGGGGAGTGTCCTTATGACCAAGGCGGGTATTTCATTATTAATGGGAGTGAGAAGGTGTTGATTGCTCAGGAGAAGATGAGCACGAACCACGTCTATGTCTTCAAGAAGAGGCAGCCTAATAAGTATGCTTATGTTGGGGAGGTACGGTCTATGGCGGAGTCCCAAAACCGACCCCCCAGCACCATGTTCGTTCGTATGCTTTCCCGGACGAGCTCCAAAGGGGGTTCTTCAGGACAATACATTCGTGCTACTCTTCCGTATATTCGATCTGAGATTCCCATTATTATTGTCTTCCGGGCTCTTGGGTTTGTGGCAGACAAAGACATTTTGGAGCACATATGTTATGATTTTTCAGATACGCAAATGATGGAGTTGCTTCGTCCTTCCCTGGAAGAAGCATTTGTTATTCAGAATCAACAGGTGGCTCTAGATTATATTGGTAAAAGAGGTGCAACAGCTGGGGTACCTAGAGACAAGAGGATAAGGTATGCAAGAGATATTCTTCAAAAGGAAATGCTTCCACATGTTGGTGTCGGGGAGTTTTGTGAGACCAAGAAAGCATATTATTTCGGATACATCATTCACAGGCTTCTACTGTGTGCACTTGGTCGGAGAGCAGAAGATGATAGGGATCACTATGGAAATAAGAGGCTAGATCTTGCTGGCCCCTTGCTCGGAAGCTTGTTTAGATCGCTATTCAGGAAGCTGACCAAGGATGTTAAAGGTTATGTTCAGAAGTGCGTTGATAATGGAAAGGATGTCAACCTGCAATTTGCAATTAAAGCAAAAACCATTACCAGTGGTCTCAAATATTCACTTGCTACTGGGAACTGGGGGCAAGCAAATGCAGCTGGGACAAGGGCTGGAGTGTCGCAGGTTTTAAATCGTTTGACATATGCATCTACATTATCACACTTGCGAAGGTTAAATTCTCCCATAGGGCGTGAAGGGAAATTGGCCAAACCCCGGCAGTTGCACAACTCACAGTGGGGAATGATGTGTCCCGCTGAGACACCTGAAGGGCAGGCATGCGGACTTGTAAAGAACCTTGCATTGATGGTCTACATAACTGTGGGATCAGCTGCATATCCAATCTTAGAATTTTTAGAAGAATGGGGTACAGAAAATTTTGAGGAAATTTCCCCTGCTGTTATTCCCCAAGCCACAAAGATTTTTGTTAATGGCTGCTGGGTTGGCATACATCGTGATCCTGAAATGTTGGTGAGAACATTAAGGAAGCTAAGGCGGCGGGTGGATGTCAATACTGAAGTTGGGGTTGTAAGAGATATCCGTCTGAAAGAATTGCGGATATATACTGATTATGGTCGTTGCAGTCGTCCATTATTCATTGTGGACAAGCAAAGGCTGCTTATAAAGAAGAAAGATATTCATGCATTGCAGCAGAGGGAAAACCCTGAAGACGGTGGTTGGCATGATCTCGTGGCAAAAGGATTTATAGAATATATTGACACTGAAGAAGAGGAGACCACTATGATTTCGATGACAATTAATGATCTTGTACAAGCAAGGCTCAATCCTGAGGAGGCATATTCTGACACTTACACTCATTGTGAAATTCACCCGTCACTTATATTGGGTGTTTGTGCTTCAATTATACCATTTCCTGATCATAATCAGTCTCCACGTAATACATATCAATCTGCCATGGGAAAGCAAGCTATGGGAATTTATGTCACCAATTATCAGTTCCGAATGGATACACTGGCTTATGTTCTGTACTACCCTCAGAAGCCGCTTGTCACTACACGAGCCATGGAGCATCTTCACTTCCGGCAACTTCCAGCTGGAATTAATGCCATTGTTGCCATTTCTTGCTACTCTGGTTATAATCAGGAAGATTCTGTCATTATGAATCAATCCTCTATAGATCGTGGATTTTTCCGGTCACTGTTCTTCCGCTCCTATAGGGATGAGGAAAAAAAGATGGGGACCCTTGTCAAAGAAGATTTTGGACGTCCAGACAGAGCAAATACGATGGGTATGCGGCATGGTTCTTATGATAAATTGGATGATGATGGTCTTGCACCTCCTGGAACCAGGGTTTCAGGTGAGGATGTTATCATAGGCAAGACCACTCCCATTGCTCCAGAGGAGGCTCAGGGGCAAGCTGCTTCACGTTACTCACGACGTGACCATAGTATAAGCTTACGGCACAGTGAAACTGGGATTGTGGACCAGGTTTTATTGACTACCAATGCTGATGGGTTGAGGTTTGTGAAAGTGAGGGTGAGGTCTGTTCGAATTCCACAGATTGGGGATAAATTTAGCAGTAGGCATGGACAGAAGGGGACGGTGGGTATGACATATACACAAGAAGACATGCCGTGGACTGTAGAAGGAGTCACCCCTGATATTATTGTGAACCCTCATGCTATTCCTTCCCGAATGACCATTGGTCAGCTTATTGAGTGTATCATGGGGAAGGTTGCAGCACACATGGGAAAGGAAGGAGATGCTACTCCCTTTACGGATGTGACTGTGAGTTTCACTTTCTCTTCAATCATGCCTCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005665 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016591 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030054 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1023

Amino Acids

115.84

Weight (kDa)

6.12

Isoelectric Point (pI)

43.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	37 - 446	8.3e-79	RNA polymerase beta subunit
RNA_pol_Rpb2_2	PF04561	207 - 398	3.6e-55	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	471 - 535	8.3e-25	RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4	PF04566	570 - 631	7.2e-24	RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_5	PF04567	656 - 707	5.4e-18	RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6	PF00562	714 - 1011	8e-107	RNA polymerase Rpb2, domain 6

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011028)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21710
fragaria_vesca	FvH4_3g09270
malus_domestica	MD05G1313100.v1.1 MD10G1295300.v1.1
prunus_persica	Prupe.4G046700_v2.0.a1
pyrus_communis	pycom05g29100 pycom05g29110 pycom10g24710 pycom10g24720
rosa_chinensis	RchiOBHm_Chr5g0008301
rosa_laevigata	RLG00000031560
rosa_multiflora	Rmu_sc0021840.1_g000003
rosa_roxburghii	Rroxscaffold_1G00068160
rosa_rugosa	Rorug04G0434800
rosa_samantha	Rh5AG063600 Rh5BG060100 Rh5CG070400 Rh5CG070500 Rh5DG059600
rosa_wichuraiana	Rw5G005730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	401, 1910, 2385
AatII	GACGTC	1 cut(s)	2515
Acc36I	ACCTGC	2 cut(s)	1311, 1402
Acc65I	GGTACC	1 cut(s)	1000
AccB1I	GGYRCC	2 cut(s)	367, 1000
AccB7I	CCANNNNNTGG	2 cut(s)	1064, 2810
AccBSI	CCGCTC	1 cut(s)	2457
AccI	GTMKAC	1 cut(s)	1599
AccII	CGCG	1 cut(s)	74
AclWI	GGATC	3 cut(s)	1176, 1621, 1739
AcoI	YGGCCR	1 cut(s)	1494
AcsI	RAATTY	9 cut(s)	511, 1465, 1637, 1663, 1674, 2112, 2223, 2799, 2819
AcuI	CTGAAG	5 cut(s)	750, 1575, 1824, 1974, 2031
AcyI	GRCGYC	2 cut(s)	368, 2512
AdeI	CACNNNGTG	1 cut(s)	2687
AfaI	GTAC	8 cut(s)	20, 32, 499, 681, 1002, 1657, 2058, 2276
AfiI	CCNNNNNNNGG	5 cut(s)	1064, 1211, 1503, 1958, 2810
AflIII	ACRYGT	2 cut(s)	72, 1060
AgsI	TTSAA	4 cut(s)	640, 1043, 2148, 3057
AhdI	GACNNNNNGTC	1 cut(s)	2952
AjiI	CACGTC	2 cut(s)	628, 2687
AjnI	CCWGG	4 cut(s)	924, 2587, 2594, 2733
AjuI	GAANNNNNNNTTGG	2 cut(s)	2228, 2260
AleI	CACNNNNGTG	1 cut(s)	452
Alw21I	GWGCWC	4 cut(s)	620, 752, 873, 1141
Alw26I	GTCTC	8 cut(s)	239, 428, 1002, 1079, 1346, 1541, 2015, 2181
Alw44I	GTGCAC	1 cut(s)	1137
AlwI	GGATC	3 cut(s)	1176, 1621, 1739
AlwNI	CAGNNNCTG	1 cut(s)	127
Ama87I	CYCGRG	1 cut(s)	220
AoxI	GGCC	3 cut(s)	180, 1201, 1494
ApaLI	GTGCAC	1 cut(s)	1137
ApoI	RAATTY	9 cut(s)	511, 1465, 1637, 1663, 1674, 2112, 2223, 2799, 2819
ArsI	GACNNNNNNTTYG	2 cut(s)	762, 794
AseI	ATTAAT	3 cut(s)	576, 2046, 2349
Asp700I	GAANNNNTTC	1 cut(s)	1053
Asp718I	GGTACC	1 cut(s)	1000
AspLEI	GCGC	1 cut(s)	370
AspS9I	GGNCC	4 cut(s)	469, 1202, 2487, 2731
AsuC2I	CCSGG	5 cut(s)	221, 222, 742, 833, 1504
AsuII	TTCGAA	2 cut(s)	99, 2797
AvaI	CYCGRG	1 cut(s)	220
AvaII	GGWCC	3 cut(s)	469, 2487, 2731
AxyI	CCTNAGG	1 cut(s)	2076
BaeGI	GKGCMC	1 cut(s)	1141
BalI	TGGCCA	1 cut(s)	1496
BanI	GGYRCC	2 cut(s)	367, 1000
BanII	GRGCYC	2 cut(s)	752, 838
BauI	CACGAG	2 cut(s)	1976, 2304
BbsI	GAAGAC	5 cut(s)	75, 628, 820, 1962, 2880
Bbv12I	GWGCWC	4 cut(s)	620, 752, 873, 1141
BccI	CCATC	8 cut(s)	302, 410, 898, 1588, 2476, 2527, 2567, 2753
BceAI	ACGGC	2 cut(s)	2723, 2866
BcgI	CGANNNNNNTGC	4 cut(s)	706, 740, 2521, 2555
BciT130I	CCWGG	4 cut(s)	926, 2589, 2596, 2735
BciVI	GTATCC	2 cut(s)	1103, 2248
BclI	TGATCA	1 cut(s)	2164
BcnI	CCSGG	5 cut(s)	221, 222, 742, 833, 1504
BcoDI	GTCTC	8 cut(s)	239, 428, 1002, 1079, 1346, 1541, 2015, 2181
BfaI	CTAG	3 cut(s)	965, 1005, 1190
BfmI	CTRYAG	3 cut(s)	2421, 2461, 2889
BfoI	RGCGCY	1 cut(s)	371
BfuAI	ACCTGC	2 cut(s)	1311, 1402
BfuI	GTATCC	2 cut(s)	1103, 2248
BglII	AGATCT	1 cut(s)	1192
Bme1390I	CCNGG	9 cut(s)	221, 222, 742, 833, 926, 1504, 2589, 2596, 2735
Bme18I	GGWCC	3 cut(s)	469, 2487, 2731
BmeRI	GACNNNNNGTC	1 cut(s)	2952
BmeT110I	CYCGRG	1 cut(s)	220
BmgBI	CACGTC	2 cut(s)	628, 2687
BmgT120I	GGNCC	4 cut(s)	469, 1202, 2487, 2731
BmiI	GGNNCC	7 cut(s)	369, 471, 1002, 1204, 2488, 2489, 2593
BmrFI	CCNGG	9 cut(s)	221, 222, 742, 833, 926, 1504, 2589, 2596, 2735
BmrI	ACTGGG	3 cut(s)	1371, 1378, 2730
BmsI	GCATC	3 cut(s)	1445, 2326, 3001
BmuI	ACTGGG	3 cut(s)	1371, 1378, 2730
BoxI	GACNNNNGTC	1 cut(s)	2492
BpiI	GAAGAC	5 cut(s)	75, 628, 820, 1962, 2880
BpmI	CTGGAG	2 cut(s)	1422, 2628
Bpu10I	CCTNAGC	3 cut(s)	603, 1778, 2655
Bpu14I	TTCGAA	2 cut(s)	99, 2797
BpuMI	CCSGG	5 cut(s)	221, 222, 742, 833, 1504
BsaAI	YACGTR	1 cut(s)	2183
BsaBI	GATNNNNATC	2 cut(s)	1167, 2169
BsaHI	GRCGYC	2 cut(s)	368, 2512
BsaI	GGTCTC	3 cut(s)	1079, 1346, 2015
BsaJI	CCNNGG	9 cut(s)	220, 556, 924, 1248, 1502, 2202, 2310, 2595, 2882
BsaWI	WCCGGW	1 cut(s)	2439
BsaXI	ACNNNNNCTCC	2 cut(s)	668, 698
Bsc4I	CCNNNNNNNGG	5 cut(s)	1064, 1211, 1503, 1958, 2810
Bse1I	ACTGG	5 cut(s)	1335, 1366, 1373, 2265, 2725
Bse21I	CCTNAGG	1 cut(s)	2076
Bse3DI	GCAATG	2 cut(s)	1931, 2637
Bse8I	GATNNNNATC	2 cut(s)	1167, 2169
BseBI	CCWGG	4 cut(s)	926, 2589, 2596, 2735
BseDI	CCNNGG	9 cut(s)	220, 556, 924, 1248, 1502, 2202, 2310, 2595, 2882
BseGI	GGATG	9 cut(s)	313, 388, 1258, 1300, 1798, 2473, 2572, 2617, 3034
BseJI	GATNNNNATC	2 cut(s)	1167, 2169
BseLI	CCNNNNNNNGG	5 cut(s)	1064, 1211, 1503, 1958, 2810
BseMI	GCAATG	2 cut(s)	1931, 2637
BseNI	ACTGG	5 cut(s)	1335, 1366, 1373, 2265, 2725
BseRI	GAGGAG	4 cut(s)	53, 2033, 2093, 2663
BseSI	GKGCMC	1 cut(s)	1141
BseYI	CCCAGC	5 cut(s)	535, 713, 995, 1388, 1725
Bsh1236I	CGCG	1 cut(s)	74
BshFI	GGCC	3 cut(s)	182, 1203, 1496
BshNI	GGYRCC	2 cut(s)	367, 1000
BsiHKAI	GWGCWC	4 cut(s)	620, 752, 873, 1141
BsiHKCI	CYCGRG	1 cut(s)	220
BsiSI	CCGG	7 cut(s)	14, 221, 742, 832, 1504, 2329, 2440
BslFI	GGGAC	6 cut(s)	455, 682, 1405, 1524, 2500, 2860
BslI	CCNNNNNNNGG	5 cut(s)	1064, 1211, 1503, 1958, 2810
BsmAI	GTCTC	8 cut(s)	239, 428, 1002, 1079, 1346, 1541, 2015, 2181
BsmFI	GGGAC	6 cut(s)	455, 682, 1405, 1524, 2500, 2860
BsnI	GGCC	3 cut(s)	182, 1203, 1496
Bso31I	GGTCTC	3 cut(s)	1079, 1346, 2015
BsoBI	CYCGRG	1 cut(s)	220
Bsp119I	TTCGAA	2 cut(s)	99, 2797
Bsp1286I	GDGCHC	5 cut(s)	620, 752, 838, 873, 1141
Bsp1407I	TGTACA	2 cut(s)	497, 2056
Bsp19I	CCATGG	2 cut(s)	2202, 2310
BspANI	GGCC	3 cut(s)	182, 1203, 1496
BspFNI	CGCG	1 cut(s)	74
BspLI	GGNNCC	7 cut(s)	369, 471, 1002, 1204, 2488, 2489, 2593
BspMI	ACCTGC	2 cut(s)	1311, 1402
BspPI	GGATC	3 cut(s)	1176, 1621, 1739
BspQI	GCTCTTC	2 cut(s)	3, 18
BspT104I	TTCGAA	2 cut(s)	99, 2797
BspT107I	GGYRCC	2 cut(s)	367, 1000
BspTNI	GGTCTC	3 cut(s)	1079, 1346, 2015
BsrBI	CCGCTC	1 cut(s)	2457
BsrDI	GCAATG	2 cut(s)	1931, 2637
BsrGI	TGTACA	2 cut(s)	497, 2056
BsrI	ACTGG	5 cut(s)	1335, 1366, 1373, 2265, 2725
BssECI	CCNNGG	9 cut(s)	220, 556, 924, 1248, 1502, 2202, 2310, 2595, 2882
BssNI	GRCGYC	2 cut(s)	368, 2512
BssSI	CACGAG	2 cut(s)	1976, 2304
BssT1I	CCWWGG	4 cut(s)	556, 1248, 2202, 2310
Bst2BI	CACGAG	2 cut(s)	1976, 2304
Bst2UI	CCWGG	4 cut(s)	926, 2589, 2596, 2735
Bst6I	CTCTTC	7 cut(s)	3, 18, 96, 638, 795, 2010, 3058
BstACI	GRCGYC	2 cut(s)	368, 2512
BstAPI	GCANNNNNTGC	1 cut(s)	1903
BstAUI	TGTACA	2 cut(s)	497, 2056
BstBAI	YACGTR	1 cut(s)	2183
BstBI	TTCGAA	2 cut(s)	99, 2797
BstC8I	GCNNGC	6 cut(s)	1201, 1377, 1563, 1567, 2214, 2664
BstDSI	CCRYGG	3 cut(s)	2202, 2310, 2882
BstEII	GGTNACC	1 cut(s)	224
BstF5I	GGATG	9 cut(s)	313, 388, 1258, 1300, 1798, 2473, 2572, 2617, 3034
BstFNI	CGCG	1 cut(s)	74
BstH2I	RGCGCY	1 cut(s)	371
BstHHI	GCGC	1 cut(s)	370
BstMAI	GTCTC	8 cut(s)	239, 428, 1002, 1079, 1346, 1541, 2015, 2181
BstMWI	GCNNNNNNNGC	6 cut(s)	1319, 1385, 1731, 1783, 1903, 2360
BstNI	CCWGG	4 cut(s)	926, 2589, 2596, 2735
BstNSI	RCATGY	3 cut(s)	1064, 1569, 2881
BstPAI	GACNNNNGTC	1 cut(s)	2492
BstPI	GGTNACC	1 cut(s)	224
BstSCI	CCNGG	9 cut(s)	219, 220, 740, 831, 924, 1502, 2587, 2594, 2733
BstSFI	CTRYAG	3 cut(s)	2421, 2461, 2889
BstSLI	GKGCMC	1 cut(s)	1141
BstUI	CGCG	1 cut(s)	74
BstV2I	GAAGAC	5 cut(s)	75, 628, 820, 1962, 2880
BstX2I	RGATCY	1 cut(s)	1192
BstYI	RGATCY	1 cut(s)	1192
Bsu36I	CCTNAGG	1 cut(s)	2076
BsuI	GTATCC	2 cut(s)	1103, 2248
BsuRI	GGCC	3 cut(s)	182, 1203, 1496
BtgI	CCRYGG	3 cut(s)	2202, 2310, 2882
BtrI	CACGTC	2 cut(s)	628, 2687
BtsCI	GGATG	9 cut(s)	313, 388, 1258, 1300, 1798, 2473, 2572, 2617, 3034
BtsIMutI	CAGTG	6 cut(s)	1342, 1529, 2007, 2258, 2444, 2719
BveI	ACCTGC	2 cut(s)	1311, 1402
Cac8I	GCNNGC	6 cut(s)	1201, 1377, 1563, 1567, 2214, 2664
CaiI	CAGNNNCTG	1 cut(s)	127
CfoI	GCGC	1 cut(s)	370
Cfr13I	GGNCC	4 cut(s)	469, 1202, 2487, 2731
Cfr9I	CCCGGG	1 cut(s)	220
CseI	GACGC	1 cut(s)	80
CsiI	ACCWGGT	1 cut(s)	2733
Csp6I	GTAC	8 cut(s)	19, 31, 498, 680, 1001, 1656, 2057, 2275
CspCI	CAANNNNNGTGG	2 cut(s)	2620, 2655
CviQI	GTAC	8 cut(s)	19, 31, 498, 680, 1001, 1656, 2057, 2275
DinI	GGCGCC	1 cut(s)	369
DraI	TTTAAA	1 cut(s)	1419
DraIII	CACNNNGTG	1 cut(s)	2687
DriI	GACNNNNNGTC	1 cut(s)	2952
EaeI	YGGCCR	1 cut(s)	1494
Eam1104I	CTCTTC	7 cut(s)	3, 18, 96, 638, 795, 2010, 3058
Eam1105I	GACNNNNNGTC	1 cut(s)	2952
EarI	CTCTTC	7 cut(s)	3, 18, 96, 638, 795, 2010, 3058
EciI	GGCGGA	1 cut(s)	707
Ecl136II	GAGCTC	1 cut(s)	750
Eco130I	CCWWGG	4 cut(s)	556, 1248, 2202, 2310
Eco24I	GRGCYC	2 cut(s)	752, 838
Eco31I	GGTCTC	3 cut(s)	1079, 1346, 2015
Eco32I	GATATC	2 cut(s)	190, 1825
Eco47I	GGWCC	3 cut(s)	469, 2487, 2731
Eco53kI	GAGCTC	1 cut(s)	750
Eco57I	CTGAAG	5 cut(s)	750, 1575, 1824, 1974, 2031
Eco81I	CCTNAGG	1 cut(s)	2076
Eco88I	CYCGRG	1 cut(s)	220
Eco91I	GGTNACC	1 cut(s)	224
EcoICRI	GAGCTC	1 cut(s)	750
EcoO109I	RGGNCCY	2 cut(s)	469, 2487
EcoO65I	GGTNACC	1 cut(s)	224
EcoRI	GAATTC	2 cut(s)	511, 2799
EcoRII	CCWGG	4 cut(s)	924, 2587, 2594, 2733
EcoRV	GATATC	2 cut(s)	190, 1825
EcoT14I	CCWWGG	4 cut(s)	556, 1248, 2202, 2310
EcoT22I	ATGCAT	2 cut(s)	1438, 1933
EcoT38I	GRGCYC	2 cut(s)	752, 838
EgeI	GGCGCC	1 cut(s)	369
EheI	GGCGCC	1 cut(s)	369
ErhI	CCWWGG	4 cut(s)	556, 1248, 2202, 2310
FaqI	GGGAC	6 cut(s)	455, 682, 1405, 1524, 2500, 2860
FauI	CCCGC	3 cut(s)	554, 1549, 1779
FauNDI	CATATG	2 cut(s)	875, 1432
FbaI	TGATCA	1 cut(s)	2164
FblI	GTMKAC	1 cut(s)	1599
FokI	GGATG	9 cut(s)	320, 395, 1265, 1307, 1805, 2480, 2579, 2624, 3041
FriOI	GRGCYC	2 cut(s)	752, 838
FspBI	CTAG	3 cut(s)	965, 1005, 1190
GlaI	GCGC	1 cut(s)	369
GsaI	CCCAGC	5 cut(s)	539, 717, 999, 1392, 1729
GsuI	CTGGAG	2 cut(s)	1422, 2628
HaeII	RGCGCY	1 cut(s)	371
HaeIII	GGCC	3 cut(s)	182, 1203, 1496
HapII	CCGG	7 cut(s)	14, 221, 742, 832, 1504, 2329, 2440
HgaI	GACGC	1 cut(s)	80
HhaI	GCGC	1 cut(s)	370
Hin1I	GRCGYC	2 cut(s)	368, 2512
Hin6I	GCGC	1 cut(s)	368
HinP1I	GCGC	1 cut(s)	368
HincII	GTYRAC	1 cut(s)	1300
HindII	GTYRAC	1 cut(s)	1300
HindIII	AAGCTT	2 cut(s)	1216, 2701
HpaII	CCGG	7 cut(s)	14, 221, 742, 832, 1504, 2329, 2440
Hpy166II	GTNNAC	7 cut(s)	1139, 1300, 1600, 1891, 2731, 2886, 2920
Hpy8I	GTNNAC	7 cut(s)	1139, 1300, 1600, 1891, 2731, 2886, 2920
Hpy99I	CGWCG	2 cut(s)	50, 2688
HpyCH4IV	ACGT	5 cut(s)	627, 2182, 2512, 2674, 2686
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1319, 1385, 1731, 1783, 1903, 2360
HpySE526I	ACGT	5 cut(s)	627, 2182, 2512, 2674, 2686
Hsp92I	GRCGYC	2 cut(s)	368, 2512
HspAI	GCGC	1 cut(s)	368
KasI	GGCGCC	1 cut(s)	367
KflI	GGGWCCC	1 cut(s)	2487
KpnI	GGTACC	1 cut(s)	1004
Ksp22I	TGATCA	1 cut(s)	2164
LguI	GCTCTTC	2 cut(s)	3, 18
LmnI	GCTCC	7 cut(s)	486, 532, 755, 868, 2314, 2462, 2647
LweI	GCATC	3 cut(s)	1445, 2326, 3001
MabI	ACCWGGT	1 cut(s)	2733
MaeI	CTAG	3 cut(s)	965, 1005, 1190
MaeII	ACGT	5 cut(s)	627, 2182, 2512, 2674, 2686
MbiI	CCGCTC	1 cut(s)	2457
MflI	RGATCY	1 cut(s)	1192
MhlI	GDGCHC	5 cut(s)	620, 752, 838, 873, 1141
MlsI	TGGCCA	1 cut(s)	1496
MluI	ACGCGT	1 cut(s)	72
MluNI	TGGCCA	1 cut(s)	1496
Mly113I	GGCGCC	1 cut(s)	368
MlyI	GAGTC	5 cut(s)	125, 311, 430, 704, 2907
MmeI	TCCRAC	3 cut(s)	223, 285, 1127
Mox20I	TGGCCA	1 cut(s)	1496
Mph1103I	ATGCAT	2 cut(s)	1438, 1933
MroXI	GAANNNNTTC	1 cut(s)	1053
MscI	TGGCCA	1 cut(s)	1496
MseI	TTAA	9 cut(s)	576, 1257, 1317, 1418, 1463, 1716, 1769, 2046, 2349
MslI	CAYNNNNRTG	5 cut(s)	417, 452, 630, 1065, 1607
Msp20I	TGGCCA	1 cut(s)	1496
MspA1I	CMGCKG	6 cut(s)	127, 995, 1388, 1544, 1619, 2342
MspI	CCGG	7 cut(s)	14, 221, 742, 832, 1504, 2329, 2440
MspR9I	CCNGG	9 cut(s)	221, 222, 742, 833, 926, 1504, 2589, 2596, 2735
MvaI	CCWGG	4 cut(s)	926, 2589, 2596, 2735
MvnI	CGCG	1 cut(s)	74
MwoI	GCNNNNNNNGC	6 cut(s)	1319, 1385, 1731, 1783, 1903, 2360
NarI	GGCGCC	1 cut(s)	368
NciI	CCSGG	5 cut(s)	221, 222, 742, 833, 1504
NcoI	CCATGG	2 cut(s)	2202, 2310
NdeI	CATATG	2 cut(s)	875, 1432
NlaIV	GGNNCC	7 cut(s)	369, 471, 1002, 1204, 2488, 2489, 2593
NmeAIII	GCCGAG	1 cut(s)	344
NmuCI	GTSAC	9 cut(s)	224, 385, 2121, 2230, 2296, 2442, 2687, 2899, 3031
NsiI	ATGCAT	2 cut(s)	1438, 1933
NspI	RCATGY	3 cut(s)	1064, 1569, 2881
NspV	TTCGAA	2 cut(s)	99, 2797
OliI	CACNNNNGTG	1 cut(s)	452
PaeI	GCATGC	1 cut(s)	1569
PciI	ACATGT	1 cut(s)	1060
PciSI	GCTCTTC	2 cut(s)	3, 18
PcsI	WCGNNNNNNNCGW	4 cut(s)	42, 329, 1870, 2680
PdmI	GAANNNNTTC	1 cut(s)	1053
PfeI	GAWTC	5 cut(s)	206, 811, 949, 2396, 2410
PflFI	GACNNNGTC	1 cut(s)	301
PflMI	CCANNNNNTGG	2 cut(s)	1064, 2810
PfoI	TCCNGGA	2 cut(s)	740, 2587
PleI	GAGTC	5 cut(s)	125, 310, 430, 703, 2906
PluTI	GGCGCC	1 cut(s)	371
PpsI	GAGTC	5 cut(s)	125, 310, 430, 703, 2906
Ppu21I	YACGTR	1 cut(s)	2183
PpuMI	RGGWCCY	2 cut(s)	469, 2487
PscI	ACATGT	1 cut(s)	1060
PshAI	GACNNNNGTC	1 cut(s)	2492
PshBI	ATTAAT	3 cut(s)	576, 2046, 2349
PsiI	TTATAA	3 cut(s)	401, 1910, 2385
Psp124BI	GAGCTC	1 cut(s)	752
Psp5II	RGGWCCY	2 cut(s)	469, 2487
Psp6I	CCWGG	4 cut(s)	924, 2587, 2594, 2733
PspEI	GGTNACC	1 cut(s)	224
PspFI	CCCAGC	5 cut(s)	535, 713, 995, 1388, 1725
PspGI	CCWGG	4 cut(s)	924, 2587, 2594, 2733
PspN4I	GGNNCC	7 cut(s)	369, 471, 1002, 1204, 2488, 2489, 2593
PspPI	GGNCC	4 cut(s)	469, 1202, 2487, 2731
PspPPI	RGGWCCY	2 cut(s)	469, 2487
PstNI	CAGNNNCTG	1 cut(s)	127
PsuI	RGATCY	1 cut(s)	1192
PsyI	GACNNNGTC	1 cut(s)	301
PvuII	CAGCTG	5 cut(s)	127, 995, 1388, 1619, 2342
RsaI	GTAC	8 cut(s)	20, 32, 499, 681, 1002, 1657, 2058, 2276
RsaNI	GTAC	8 cut(s)	19, 31, 498, 680, 1001, 1656, 2057, 2275
RseI	CAYNNNNRTG	5 cut(s)	417, 452, 630, 1065, 1607
SacI	GAGCTC	1 cut(s)	752
SapI	GCTCTTC	2 cut(s)	3, 18
SaqAI	TTAA	9 cut(s)	576, 1257, 1317, 1418, 1463, 1716, 1769, 2046, 2349
Sau96I	GGNCC	4 cut(s)	469, 1202, 2487, 2731
SchI	GAGTC	5 cut(s)	125, 311, 430, 704, 2907
ScrFI	CCNGG	9 cut(s)	221, 222, 742, 833, 926, 1504, 2589, 2596, 2735
SduI	GDGCHC	5 cut(s)	620, 752, 838, 873, 1141
SexAI	ACCWGGT	1 cut(s)	2733
SfaNI	GCATC	3 cut(s)	1445, 2326, 3001
SfcI	CTRYAG	3 cut(s)	2421, 2461, 2889
SfoI	GGCGCC	1 cut(s)	369
SfuI	TTCGAA	2 cut(s)	99, 2797
SinI	GGWCC	3 cut(s)	469, 2487, 2731
SmaI	CCCGGG	1 cut(s)	222
SmiMI	CAYNNNNRTG	5 cut(s)	417, 452, 630, 1065, 1607
SphI	GCATGC	1 cut(s)	1569
SspDI	GGCGCC	1 cut(s)	367
SspI	AATATT	1 cut(s)	1349
SspMI	CTAG	3 cut(s)	965, 1005, 1190
SstI	GAGCTC	1 cut(s)	752
StyD4I	CCNGG	9 cut(s)	219, 220, 740, 831, 924, 1502, 2587, 2594, 2733
StyI	CCWWGG	4 cut(s)	556, 1248, 2202, 2310
TaiI	ACGT	5 cut(s)	630, 2185, 2515, 2677, 2689
TaqI	TCGA	8 cut(s)	99, 138, 174, 192, 488, 802, 2036, 2797
TaqII	GACCGA	2 cut(s)	257, 314
TatI	WGTACW	4 cut(s)	30, 497, 2056, 2274
TauI	GCSGC	4 cut(s)	185, 1786, 2293, 2545
TfiI	GAWTC	5 cut(s)	206, 811, 949, 2396, 2410
Tru1I	TTAA	9 cut(s)	576, 1257, 1317, 1418, 1463, 1716, 1769, 2046, 2349
Tru9I	TTAA	9 cut(s)	576, 1257, 1317, 1418, 1463, 1716, 1769, 2046, 2349
TscAI	CASTG	6 cut(s)	1342, 1529, 2014, 2265, 2451, 2719
TseFI	GTSAC	9 cut(s)	224, 385, 2121, 2230, 2296, 2442, 2687, 2899, 3031
Tsp45I	GTSAC	9 cut(s)	224, 385, 2121, 2230, 2296, 2442, 2687, 2899, 3031
TspDTI	ATGAA	6 cut(s)	136, 448, 559, 1873, 1916, 2423
TspGWI	ACGGA	5 cut(s)	473, 545, 783, 1817, 3041
TspMI	CCCGGG	1 cut(s)	220
TspRI	CASTG	6 cut(s)	1342, 1529, 2014, 2265, 2451, 2719
Tth111I	GACNNNGTC	1 cut(s)	301
Van91I	CCANNNNNTGG	2 cut(s)	1064, 2810
VneI	GTGCAC	1 cut(s)	1137
VpaK11BI	GGWCC	3 cut(s)	469, 2487, 2731
VspI	ATTAAT	3 cut(s)	576, 2046, 2349
XapI	RAATTY	9 cut(s)	511, 1465, 1637, 1663, 1674, 2112, 2223, 2799, 2819
XbaI	TCTAGA	1 cut(s)	964
XceI	RCATGY	3 cut(s)	1064, 1569, 2881
XmaI	CCCGGG	1 cut(s)	220
XmiI	GTMKAC	1 cut(s)	1599
XmnI	GAANNNNTTC	1 cut(s)	1053
XspI	CTAG	3 cut(s)	965, 1005, 1190
ZraI	GACGTC	1 cut(s)	2513
Zsp2I	ATGCAT	2 cut(s)	1438, 1933

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.