Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0011028

Physical Location & Seq

Genomic Coordinates Reverse (-)

6190487 .. 6196762

Gene Structure 6276 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031560

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3336 bp

ATGGAAGAAGAGCCGGAGTACGAAGAGCAGTACAACGAGGAGGACGACGAAGATGAGATAACGCAGGAAGACGCGTGGGCTGTGATTTCAGCCTATTTCGAAGAGAAGGGTTTAGTGAGGCAGCAGCTGGACTCGTTCGATGAGTTCATTCAGAACACAATGCAGGAGATTGTCGACGAGGCCGCCGATATCGAAATCCGACCCGAATCGCAGCATAACCCGGGTCACCAGCCCGATTTCGCTGAGACTATATATAAAATCAGCTTCGGTCAGATTTATCTTAGTAAGCCTATGATGACTGAGTCGGATGGTGAGACTGCAACACTGTTTCCGAAAGCTGCAAGGTTGCGAAACTTGACCTACTCGGCGCCTTTGTATGTGGATGTGACAAAGAGGGTGATAAAGAAAGGACATGATGGTGAAGAAGTTACTGAGACTCAGGATTTCACCAAAGTGTTCATTGGAAAGGTCCCAATTATGCTCCGTTCGAGTTATTGTACATTGTATCAGAACTCGGAGAAAGACTTGACGGAGCTGGGGGAGTGTCCTTATGACCAAGGCGGGTATTTCATTATTAATGGGAGTGAGAAGGTGTTGATTGCTCAGGAGAAGATGAGCACGAACCACGTCTATGTCTTCAAGAAGAGGCAGCCTAATAAGTATGCTTATGTTGGGGAGGTACGGTCTATGGCGGAGTCCCAAAACCGACCCCCCAGCACCATGTTCGTTCGTATGCTTTCCCGGACGAGCTCCAAAGGGGGTTCTTCAGGACAATACATTCGTGCTACTCTTCCGTATATACGATCTGAGATTCCCATTATTATTGTCTTCCGGGCTCTTGGGTTTGTGGCAGACAAAGACATTTTGGAGCACATATGCTATGATTTTTCAGATACGCAAATGATGGAGTTGCTTCGTCCTTCCCTGGAAGAAGCATTTGTTATTCAGAATCAACAGGTGGCTCTAGATTATATTGGTAAAAGAGGTGCAACAGCTGGGGTACCTAGAGACAAGAGGATAAGGTATGCGAGAGATATTCTTCAAAAGGAAATGCTTCCACATGTTGGTGTTGGAGAGTTTTGTGAGACCAAGAAAGCATATTATTTCGGATACATCATTCACAGGCTTCTACTGTGTGCACTTGGTCGGAGAGCAGAAGATGATAGGGATCACTATGGAAACAAGAGGCTAGATCTTGCTGGCCCCTTACTCGGAAGCTTGTTTAGATCGCTATTCAGGAAGCTGACCAAGGATGTTAAAGGTTATGTGCAGAAGTGCGTTGATAATGGAAAGGATGTCAACCTGCAATTTGCAATTAAAGCAAAAACCATTACCAGTGGTCTCAAATATTCACTTGCTACTGGGAACTGGGGGCAAGCAAATGCAGCTGGGACAAGAGCTGGAGTGTCCCAGGTGTTAAATCGTTTGACATATGCATCTACATTATCACACTTGCGAAGGCTAAATTCTCCCATAGGGCGTGAAGGGAAATTGGCCAAACCCCGGCAGTTGCACAATTCACAGTGGGGAATGATGTGTCCTGCTGAGACACCTGAAGGGCAGGCATGCGGACTTGTAAAGAACCTTGCATTGATGGTCTACATAACTGTGGGATCAGCTGCATATCCAATTTTAGAATTTTTAGAAGAATGGGGTACAGAAAATTTTGAGGAAATTTCCCCCGCTGTTATTCCCCAAGCCACAAAGATTTTTGTTAATGGCTGCTGGGTTGGCATACATCGTGATCCCGAAATGTTGGTGAGAACATTAAGGAAGCTAAGGCGGCGGGTGGATGTCAATACTGAAGTTGGGGTTGTAAGAGATATCCGTCTGAAAGAATTGCGGATATATACTGATTATGGTCGTTGCAGTCGTCCATTATTCATTGTGGACAAGCAAAGGCTGCTTATAAAGAAGAAAGATATTCATGCATTGCAGCAGAGGGAGGCATATTCTGACACTTACACTCATTGCGAAATTCACCCGTCTCTTATATTGGGTTCTCCACGTAATACATATCAATCTGCCATGGGAAAGCAAGCTATGGGAATTTATGTCACCAATTATCAGTTCCGAATGGATACACTGGCTTATGTTCTGTACTACCCACAGAAGCCGCTTGTCACTACACGAGCCATGGAGCATCTTCACTTCCGGCAACTTCCAGCTGGAATTAATGCCATTGTTGCGATTTCCTGCTACTCTGGTTATAATCAGGAAGATTCTGTCATTATGAATCAATCCTCTATAGATCGTGGATTTTTCCGGTCACTGTTCTTCCGCTCCTATAGGGATGAGGAAAAAAAGATGGGGACCCTTGTCAAAGAAGATTTCGGACGTCCAGACAGAGGAACCAGGGTTTCAGGTGAGGATGTTATCATAGGGAAGACCACTCCCATTGCTCCGGAGGAGGCTCAGGGGCAAGCTGCTTCGCGTTACTCACGACGTGACCATAGTATAAGCTTACGGCACAGTGAAACTGGGATTGTGGACCAGGTTTTATTGACTACCAATGCCGATGGGTTGAGGTTTGTGAAAGTGAGGGTGAGGTCTGTTCGAATTCCACAGATTGGGGATAAATTTAGCAGTAGGCATGGACAGAAAGGGACAGTGGGTATGACATATACACAAGAAGACATGCCATGGACTGTAGAAGGAGTCACCCCTGATATTATTGTGAACCCTCATGCTATTCCTTCCCGAATGACCATTGGTCAGCTTATTGAGTGTATCATGGGGAAGGTTGCAGCACACATGGGAAAGGAAGGAGATGCTACTCCCTTTACAGATGTGACTGTGGACAATATCAGCAAAGCTCTTCACAAATGTGGGTATCAAATGCGGGGATTTGAGACCATGTATAATGGTCACACTGGTCGGCGACTGAGTGCAATGATATTCCTTGGCCCTACATATTACCAGAGATTGAAGCATATGGTTGATGATAAGATCCATTCACGTGGTCGGGGTCCTGTACAGATCCTCACAAGGCAGCCGGCTGAGGGACGGTCTCGTGATGGTGGTTTACGATTTGGAGAGATGGAACGAGATTGCATGATTGCCCACGGTGCTGCTCACTTTCTTAAAGAGAGATTGTTTGATCAGAGTGATGCCTATAGGGTTCATGTCTGCGAGCGTTGTGGTTTAATAGCTATTGCAAATCTCAAGAAGAATTCATTCGAGTGCAGAGGTTGTAAGAATAAAACTGATATTGTTCAGGTTCACATTCCTTATGCCTGCAAGCTGCTATTCCAAGAGCTTATGGCCATGGCTATAGCACCAAGAATGCTCACAAAGGATGTCAAGCCTATCAAAGAAAAGAAGAAAGGAGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005665 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016591 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030054 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1112

Amino Acids

126.14

Weight (kDa)

8.77

Isoelectric Point (pI)

40.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	37 - 446	9.9e-79	RNA polymerase beta subunit
RNA_pol_Rpb2_2	PF04561	207 - 398	4.1e-55	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	471 - 535	9.2e-25	RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4	PF04566	570 - 631	7.9e-24	RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_6	PF00562	668 - 1005	1e-125	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7	PF04560	1007 - 1098	1.5e-35	RNA polymerase Rpb2, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011028)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21710
fragaria_vesca	FvH4_3g09270
malus_domestica	MD05G1313100.v1.1 MD10G1295300.v1.1
prunus_persica	Prupe.4G046700_v2.0.a1
pyrus_communis	pycom05g29100 pycom05g29110 pycom10g24710 pycom10g24720
rosa_chinensis	RchiOBHm_Chr5g0008301
rosa_laevigata	RLG00000031560
rosa_multiflora	Rmu_sc0021840.1_g000003
rosa_roxburghii	Rroxscaffold_1G00068160
rosa_rugosa	Rorug04G0434800
rosa_samantha	Rh5AG063600 Rh5BG060100 Rh5CG070400 Rh5CG070500 Rh5DG059600
rosa_wichuraiana	Rw5G005730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1910, 2211
AatII	GACGTC	1 cut(s)	2341
Acc36I	ACCTGC	1 cut(s)	1311
Acc65I	GGTACC	1 cut(s)	1000
AccB1I	GGYRCC	2 cut(s)	367, 1000
AccB7I	CCANNNNNTGG	2 cut(s)	1064, 2570
AccBSI	CCGCTC	1 cut(s)	2283
AccI	GTMKAC	2 cut(s)	174, 1599
AccII	CGCG	2 cut(s)	74, 2434
AccIII	TCCGGA	1 cut(s)	2404
AclWI	GGATC	5 cut(s)	1176, 1621, 1739, 2944, 2974
AcoI	YGGCCR	2 cut(s)	1494, 3264
AcsI	RAATTY	9 cut(s)	1465, 1637, 1663, 1674, 1977, 2049, 2559, 2579, 3172
AcuI	CTGAAG	3 cut(s)	750, 1575, 1824
AcvI	CACGTG	1 cut(s)	2960
AcyI	GRCGYC	2 cut(s)	368, 2338
AdeI	CACNNNGTG	1 cut(s)	2447
AfaI	GTAC	8 cut(s)	20, 32, 499, 681, 1002, 1657, 2102, 2976
AfiI	CCNNNNNNNGG	6 cut(s)	1064, 1211, 1503, 2348, 2570, 3002
AflIII	ACRYGT	2 cut(s)	72, 1060
AgsI	TTSAA	3 cut(s)	640, 1043, 2929
AhdI	GACNNNNNGTC	1 cut(s)	2712
AjiI	CACGTC	2 cut(s)	628, 2447
AjnI	CCWGG	4 cut(s)	924, 1410, 2354, 2493
AjuI	GAANNNNNNNTTGG	2 cut(s)	2054, 2086
AleI	CACNNNNGTG	2 cut(s)	452, 2826
Alw21I	GWGCWC	4 cut(s)	620, 752, 873, 1141
Alw44I	GTGCAC	1 cut(s)	1137
AlwI	GGATC	5 cut(s)	1176, 1621, 1739, 2944, 2974
AlwNI	CAGNNNCTG	1 cut(s)	127
Ama87I	CYCGRG	1 cut(s)	220
Aor13HI	TCCGGA	1 cut(s)	2404
AoxI	GGCC	5 cut(s)	180, 1201, 1494, 2905, 3264
ApaLI	GTGCAC	1 cut(s)	1137
ApoI	RAATTY	9 cut(s)	1465, 1637, 1663, 1674, 1977, 2049, 2559, 2579, 3172
ArsI	GACNNNNNNTTYG	2 cut(s)	762, 794
AseI	ATTAAT	2 cut(s)	576, 2175
Asp700I	GAANNNNTTC	2 cut(s)	1053, 3176
Asp718I	GGTACC	1 cut(s)	1000
AspLEI	GCGC	1 cut(s)	370
AspS9I	GGNCC	6 cut(s)	469, 1202, 2313, 2491, 2906, 2969
AsuC2I	CCSGG	5 cut(s)	221, 222, 742, 833, 1504
AsuII	TTCGAA	2 cut(s)	99, 2557
AvaI	CYCGRG	1 cut(s)	220
AvaII	GGWCC	4 cut(s)	469, 2313, 2491, 2969
BaeGI	GKGCMC	1 cut(s)	1141
BalI	TGGCCA	2 cut(s)	1496, 3266
BanI	GGYRCC	2 cut(s)	367, 1000
BanII	GRGCYC	2 cut(s)	752, 838
BauI	CACGAG	2 cut(s)	2130, 3012
BbrPI	CACGTG	1 cut(s)	2960
BbsI	GAAGAC	5 cut(s)	75, 628, 820, 2393, 2640
Bbv12I	GWGCWC	4 cut(s)	620, 752, 873, 1141
BbvCI	CCTCAGC	1 cut(s)	3000
BccI	CCATC	8 cut(s)	302, 410, 898, 1588, 2302, 2513, 3011, 3034
BceAI	ACGGC	1 cut(s)	2483
BcgI	CGANNNNNNTGC	2 cut(s)	706, 740
BciT130I	CCWGG	4 cut(s)	926, 1412, 2356, 2495
BciVI	GTATCC	2 cut(s)	1103, 2074
BclI	TGATCA	1 cut(s)	3100
BcnI	CCSGG	5 cut(s)	221, 222, 742, 833, 1504
BfaI	CTAG	3 cut(s)	965, 1005, 1190
BfmI	CTRYAG	5 cut(s)	2247, 2287, 2649, 3115, 3273
BfoI	RGCGCY	1 cut(s)	371
BfuAI	ACCTGC	1 cut(s)	1311
BfuI	GTATCC	2 cut(s)	1103, 2074
BglII	AGATCT	1 cut(s)	1192
Bme1390I	CCNGG	9 cut(s)	221, 222, 742, 833, 926, 1412, 1504, 2356, 2495
Bme18I	GGWCC	4 cut(s)	469, 2313, 2491, 2969
BmeRI	GACNNNNNGTC	1 cut(s)	2712
BmeT110I	CYCGRG	1 cut(s)	220
BmgBI	CACGTC	2 cut(s)	628, 2447
BmgT120I	GGNCC	6 cut(s)	469, 1202, 2313, 2491, 2906, 2969
BmiI	GGNNCC	9 cut(s)	369, 471, 1002, 1204, 2314, 2315, 2353, 2970, 3331
BmrFI	CCNGG	9 cut(s)	221, 222, 742, 833, 926, 1412, 1504, 2356, 2495
BmrI	ACTGGG	3 cut(s)	1371, 1378, 2490
BmsI	GCATC	4 cut(s)	1445, 2152, 2761, 3100
BmuI	ACTGGG	3 cut(s)	1371, 1378, 2490
BoxI	GACNNNNGTC	1 cut(s)	2318
BpiI	GAAGAC	5 cut(s)	75, 628, 820, 2393, 2640
BpmI	CTGGAG	1 cut(s)	1422
Bpu10I	CCTNAGC	4 cut(s)	603, 1778, 2415, 3000
Bpu14I	TTCGAA	2 cut(s)	99, 2557
BpuEI	CTTGAG	1 cut(s)	3149
BpuMI	CCSGG	5 cut(s)	221, 222, 742, 833, 1504
BsaAI	YACGTR	2 cut(s)	2009, 2960
BsaBI	GATNNNNATC	1 cut(s)	1167
BsaHI	GRCGYC	2 cut(s)	368, 2338
BsaI	GGTCTC	4 cut(s)	1079, 1346, 2846, 3015
BsaWI	WCCGGW	2 cut(s)	2265, 2404
BsaXI	ACNNNNNCTCC	2 cut(s)	668, 698
Bsc4I	CCNNNNNNNGG	6 cut(s)	1064, 1211, 1503, 2348, 2570, 3002
Bse118I	RCCGGY	1 cut(s)	2995
Bse1I	ACTGG	6 cut(s)	1335, 1366, 1373, 2091, 2485, 2878
Bse3DI	GCAATG	4 cut(s)	1931, 1969, 2397, 2898
Bse8I	GATNNNNATC	1 cut(s)	1167
BseAI	TCCGGA	1 cut(s)	2404
BseBI	CCWGG	4 cut(s)	926, 1412, 2356, 2495
BseGI	GGATG	8 cut(s)	313, 388, 1258, 1300, 1798, 2299, 2377, 3304
BseJI	GATNNNNATC	1 cut(s)	1167
BseLI	CCNNNNNNNGG	6 cut(s)	1064, 1211, 1503, 2348, 2570, 3002
BseMI	GCAATG	4 cut(s)	1931, 1969, 2397, 2898
BseNI	ACTGG	6 cut(s)	1335, 1366, 1373, 2091, 2485, 2878
BseRI	GAGGAG	2 cut(s)	53, 2423
BseSI	GKGCMC	1 cut(s)	1141
BseYI	CCCAGC	5 cut(s)	535, 713, 995, 1388, 1725
BsgI	GTGCAG	2 cut(s)	1289, 3205
Bsh1236I	CGCG	2 cut(s)	74, 2434
BshFI	GGCC	5 cut(s)	182, 1203, 1496, 2907, 3266
BshNI	GGYRCC	2 cut(s)	367, 1000
BsiHKAI	GWGCWC	4 cut(s)	620, 752, 873, 1141
BsiHKCI	CYCGRG	1 cut(s)	220
BsiSI	CCGG	9 cut(s)	14, 221, 742, 832, 1504, 2155, 2266, 2405, 2996
BslFI	GGGAC	7 cut(s)	455, 682, 1393, 1405, 2326, 2620, 3018
BslI	CCNNNNNNNGG	6 cut(s)	1064, 1211, 1503, 2348, 2570, 3002
BsmBI	CGTCTC	1 cut(s)	1992
BsmFI	GGGAC	7 cut(s)	455, 682, 1393, 1405, 2326, 2620, 3018
BsmI	GAATGC	1 cut(s)	3291
BsnI	GGCC	5 cut(s)	182, 1203, 1496, 2907, 3266
Bso31I	GGTCTC	4 cut(s)	1079, 1346, 2846, 3015
BsoBI	CYCGRG	1 cut(s)	220
Bsp119I	TTCGAA	2 cut(s)	99, 2557
Bsp1286I	GDGCHC	5 cut(s)	620, 752, 838, 873, 1141
Bsp13I	TCCGGA	1 cut(s)	2404
Bsp1407I	TGTACA	2 cut(s)	497, 2974
Bsp19I	CCATGG	4 cut(s)	2028, 2136, 2642, 3267
BspANI	GGCC	5 cut(s)	182, 1203, 1496, 2907, 3266
BspEI	TCCGGA	1 cut(s)	2404
BspFNI	CGCG	2 cut(s)	74, 2434
BspLI	GGNNCC	9 cut(s)	369, 471, 1002, 1204, 2314, 2315, 2353, 2970, 3331
BspMI	ACCTGC	1 cut(s)	1311
BspPI	GGATC	5 cut(s)	1176, 1621, 1739, 2944, 2974
BspQI	GCTCTTC	3 cut(s)	3, 18, 2823
BspT104I	TTCGAA	2 cut(s)	99, 2557
BspT107I	GGYRCC	2 cut(s)	367, 1000
BspTNI	GGTCTC	4 cut(s)	1079, 1346, 2846, 3015
BsrBI	CCGCTC	1 cut(s)	2283
BsrDI	GCAATG	4 cut(s)	1931, 1969, 2397, 2898
BsrFI	RCCGGY	1 cut(s)	2995
BsrGI	TGTACA	2 cut(s)	497, 2974
BsrI	ACTGG	6 cut(s)	1335, 1366, 1373, 2091, 2485, 2878
BssAI	RCCGGY	1 cut(s)	2995
BssNI	GRCGYC	2 cut(s)	368, 2338
BssSI	CACGAG	2 cut(s)	2130, 3012
BssT1I	CCWWGG	7 cut(s)	556, 1248, 2028, 2136, 2642, 2902, 3267
Bst2BI	CACGAG	2 cut(s)	2130, 3012
Bst2UI	CCWGG	4 cut(s)	926, 1412, 2356, 2495
Bst6I	CTCTTC	6 cut(s)	3, 18, 96, 638, 795, 2823
BstACI	GRCGYC	2 cut(s)	368, 2338
BstAPI	GCANNNNNTGC	1 cut(s)	1903
BstAUI	TGTACA	2 cut(s)	497, 2974
BstBAI	YACGTR	2 cut(s)	2009, 2960
BstBI	TTCGAA	2 cut(s)	99, 2557
BstDSI	CCRYGG	5 cut(s)	2028, 2136, 2642, 3064, 3267
BstEII	GGTNACC	1 cut(s)	224
BstF5I	GGATG	8 cut(s)	313, 388, 1258, 1300, 1798, 2299, 2377, 3304
BstFNI	CGCG	2 cut(s)	74, 2434
BstH2I	RGCGCY	1 cut(s)	371
BstHHI	GCGC	1 cut(s)	370
BstMWI	GCNNNNNNNGC	7 cut(s)	1319, 1385, 1731, 1783, 1903, 2186, 3068
BstNI	CCWGG	4 cut(s)	926, 1412, 2356, 2495
BstNSI	RCATGY	3 cut(s)	1064, 1569, 2641
BstPAI	GACNNNNGTC	1 cut(s)	2318
BstPI	GGTNACC	1 cut(s)	224
BstSCI	CCNGG	9 cut(s)	219, 220, 740, 831, 924, 1410, 1502, 2354, 2493
BstSFI	CTRYAG	5 cut(s)	2247, 2287, 2649, 3115, 3273
BstSLI	GKGCMC	1 cut(s)	1141
BstUI	CGCG	2 cut(s)	74, 2434
BstV2I	GAAGAC	5 cut(s)	75, 628, 820, 2393, 2640
BstX2I	RGATCY	3 cut(s)	1192, 2949, 2979
BstXI	CCANNNNNNTGG	1 cut(s)	2960
BstYI	RGATCY	3 cut(s)	1192, 2949, 2979
BsuI	GTATCC	2 cut(s)	1103, 2074
BsuRI	GGCC	5 cut(s)	182, 1203, 1496, 2907, 3266
BtgI	CCRYGG	5 cut(s)	2028, 2136, 2642, 3064, 3267
BtrI	CACGTC	2 cut(s)	628, 2447
BtsCI	GGATG	8 cut(s)	313, 388, 1258, 1300, 1798, 2299, 2377, 3304
BtsIMutI	CAGTG	8 cut(s)	323, 1342, 1529, 2084, 2270, 2479, 2616, 2871
BveI	ACCTGC	1 cut(s)	1311
CaiI	CAGNNNCTG	1 cut(s)	127
CfoI	GCGC	1 cut(s)	370
Cfr10I	RCCGGY	1 cut(s)	2995
Cfr13I	GGNCC	6 cut(s)	469, 1202, 2313, 2491, 2906, 2969
Cfr9I	CCCGGG	1 cut(s)	220
CseI	GACGC	1 cut(s)	80
CsiI	ACCWGGT	1 cut(s)	2493
Csp6I	GTAC	8 cut(s)	19, 31, 498, 680, 1001, 1656, 2101, 2975
CspCI	CAANNNNNGTGG	2 cut(s)	2380, 2415
CviQI	GTAC	8 cut(s)	19, 31, 498, 680, 1001, 1656, 2101, 2975
DinI	GGCGCC	1 cut(s)	369
DraIII	CACNNNGTG	1 cut(s)	2447
DriI	GACNNNNNGTC	1 cut(s)	2712
EaeI	YGGCCR	2 cut(s)	1494, 3264
Eam1104I	CTCTTC	6 cut(s)	3, 18, 96, 638, 795, 2823
Eam1105I	GACNNNNNGTC	1 cut(s)	2712
EarI	CTCTTC	6 cut(s)	3, 18, 96, 638, 795, 2823
EciI	GGCGGA	1 cut(s)	707
Ecl136II	GAGCTC	1 cut(s)	750
Eco130I	CCWWGG	7 cut(s)	556, 1248, 2028, 2136, 2642, 2902, 3267
Eco24I	GRGCYC	2 cut(s)	752, 838
Eco31I	GGTCTC	4 cut(s)	1079, 1346, 2846, 3015
Eco32I	GATATC	2 cut(s)	190, 1825
Eco47I	GGWCC	4 cut(s)	469, 2313, 2491, 2969
Eco53kI	GAGCTC	1 cut(s)	750
Eco57I	CTGAAG	3 cut(s)	750, 1575, 1824
Eco72I	CACGTG	1 cut(s)	2960
Eco88I	CYCGRG	1 cut(s)	220
Eco91I	GGTNACC	1 cut(s)	224
EcoICRI	GAGCTC	1 cut(s)	750
EcoO109I	RGGNCCY	3 cut(s)	469, 2313, 2969
EcoO65I	GGTNACC	1 cut(s)	224
EcoRI	GAATTC	2 cut(s)	2559, 3172
EcoRII	CCWGG	4 cut(s)	924, 1410, 2354, 2493
EcoRV	GATATC	2 cut(s)	190, 1825
EcoT14I	CCWWGG	7 cut(s)	556, 1248, 2028, 2136, 2642, 2902, 3267
EcoT22I	ATGCAT	2 cut(s)	1438, 1933
EcoT38I	GRGCYC	2 cut(s)	752, 838
EgeI	GGCGCC	1 cut(s)	369
EheI	GGCGCC	1 cut(s)	369
ErhI	CCWWGG	7 cut(s)	556, 1248, 2028, 2136, 2642, 2902, 3267
Esp3I	CGTCTC	1 cut(s)	1992
FaqI	GGGAC	7 cut(s)	455, 682, 1393, 1405, 2326, 2620, 3018
FauI	CCCGC	4 cut(s)	554, 1690, 1779, 2835
FauNDI	CATATG	3 cut(s)	875, 1432, 2934
FbaI	TGATCA	1 cut(s)	3100
FblI	GTMKAC	2 cut(s)	174, 1599
FokI	GGATG	8 cut(s)	320, 395, 1265, 1307, 1805, 2306, 2384, 3311
FriOI	GRGCYC	2 cut(s)	752, 838
FspBI	CTAG	3 cut(s)	965, 1005, 1190
GlaI	GCGC	1 cut(s)	369
GsaI	CCCAGC	5 cut(s)	539, 717, 999, 1392, 1729
GsuI	CTGGAG	1 cut(s)	1422
HaeII	RGCGCY	1 cut(s)	371
HaeIII	GGCC	5 cut(s)	182, 1203, 1496, 2907, 3266
HapII	CCGG	9 cut(s)	14, 221, 742, 832, 1504, 2155, 2266, 2405, 2996
HgaI	GACGC	1 cut(s)	80
HhaI	GCGC	1 cut(s)	370
Hin1I	GRCGYC	2 cut(s)	368, 2338
Hin6I	GCGC	1 cut(s)	368
HinP1I	GCGC	1 cut(s)	368
HincII	GTYRAC	2 cut(s)	175, 1300
HindII	GTYRAC	2 cut(s)	175, 1300
HindIII	AAGCTT	2 cut(s)	1216, 2461
HpaII	CCGG	9 cut(s)	14, 221, 742, 832, 1504, 2155, 2266, 2405, 2996
Hpy99I	CGWCG	3 cut(s)	50, 179, 2448
HpyCH4IV	ACGT	5 cut(s)	627, 2008, 2338, 2446, 2959
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1319, 1385, 1731, 1783, 1903, 2186, 3068
HpySE526I	ACGT	5 cut(s)	627, 2008, 2338, 2446, 2959
Hsp92I	GRCGYC	2 cut(s)	368, 2338
HspAI	GCGC	1 cut(s)	368
KasI	GGCGCC	1 cut(s)	367
KflI	GGGWCCC	1 cut(s)	2313
Kpn2I	TCCGGA	1 cut(s)	2404
KpnI	GGTACC	1 cut(s)	1004
KroI	GCCGGC	1 cut(s)	2995
KroNI	GCCGGC	1 cut(s)	2997
Ksp22I	TGATCA	1 cut(s)	3100
LguI	GCTCTTC	3 cut(s)	3, 18, 2823
LmnI	GCTCC	8 cut(s)	486, 532, 755, 868, 2140, 2288, 2407, 3329
LweI	GCATC	4 cut(s)	1445, 2152, 2761, 3100
MabI	ACCWGGT	1 cut(s)	2493
MaeI	CTAG	3 cut(s)	965, 1005, 1190
MaeII	ACGT	5 cut(s)	627, 2008, 2338, 2446, 2959
MbiI	CCGCTC	1 cut(s)	2283
MflI	RGATCY	3 cut(s)	1192, 2949, 2979
MhlI	GDGCHC	5 cut(s)	620, 752, 838, 873, 1141
MlsI	TGGCCA	2 cut(s)	1496, 3266
MluI	ACGCGT	1 cut(s)	72
MluNI	TGGCCA	2 cut(s)	1496, 3266
Mly113I	GGCGCC	1 cut(s)	368
MlyI	GAGTC	5 cut(s)	125, 311, 430, 704, 2667
MmeI	TCCRAC	4 cut(s)	223, 285, 1051, 1127
Mox20I	TGGCCA	2 cut(s)	1496, 3266
Mph1103I	ATGCAT	2 cut(s)	1438, 1933
MroI	TCCGGA	1 cut(s)	2404
MroNI	GCCGGC	1 cut(s)	2995
MroXI	GAANNNNTTC	2 cut(s)	1053, 3176
MscI	TGGCCA	2 cut(s)	1496, 3266
MseI	TTAA	9 cut(s)	576, 1257, 1317, 1418, 1716, 1769, 2175, 3084, 3146
MslI	CAYNNNNRTG	8 cut(s)	417, 452, 630, 1065, 1607, 2826, 2958, 3181
Msp20I	TGGCCA	2 cut(s)	1496, 3266
MspA1I	CMGCKG	6 cut(s)	127, 995, 1388, 1619, 1685, 2168
MspI	CCGG	9 cut(s)	14, 221, 742, 832, 1504, 2155, 2266, 2405, 2996
MspR9I	CCNGG	9 cut(s)	221, 222, 742, 833, 926, 1412, 1504, 2356, 2495
Mva1269I	GAATGC	1 cut(s)	3291
MvaI	CCWGG	4 cut(s)	926, 1412, 2356, 2495
MvnI	CGCG	2 cut(s)	74, 2434
MwoI	GCNNNNNNNGC	7 cut(s)	1319, 1385, 1731, 1783, 1903, 2186, 3068
NaeI	GCCGGC	1 cut(s)	2997
NarI	GGCGCC	1 cut(s)	368
NciI	CCSGG	5 cut(s)	221, 222, 742, 833, 1504
NcoI	CCATGG	4 cut(s)	2028, 2136, 2642, 3267
NdeI	CATATG	3 cut(s)	875, 1432, 2934
NgoMIV	GCCGGC	1 cut(s)	2995
NlaIV	GGNNCC	9 cut(s)	369, 471, 1002, 1204, 2314, 2315, 2353, 2970, 3331
NmeAIII	GCCGAG	1 cut(s)	344
NmuCI	GTSAC	9 cut(s)	224, 385, 2056, 2122, 2268, 2447, 2659, 2791, 2867
NsiI	ATGCAT	2 cut(s)	1438, 1933
NspI	RCATGY	3 cut(s)	1064, 1569, 2641
NspV	TTCGAA	2 cut(s)	99, 2557
OliI	CACNNNNGTG	2 cut(s)	452, 2826
PaeI	GCATGC	1 cut(s)	1569
PciI	ACATGT	1 cut(s)	1060
PciSI	GCTCTTC	3 cut(s)	3, 18, 2823
PcsI	WCGNNNNNNNCGW	3 cut(s)	42, 183, 1870
PctI	GAATGC	1 cut(s)	3291
PdiI	GCCGGC	1 cut(s)	2997
PdmI	GAANNNNTTC	2 cut(s)	1053, 3176
PfeI	GAWTC	5 cut(s)	206, 811, 949, 2222, 2236
PflFI	GACNNNGTC	1 cut(s)	301
PflMI	CCANNNNNTGG	2 cut(s)	1064, 2570
PfoI	TCCNGGA	1 cut(s)	740
PleI	GAGTC	5 cut(s)	125, 310, 430, 703, 2666
PluTI	GGCGCC	1 cut(s)	371
PmaCI	CACGTG	1 cut(s)	2960
PmlI	CACGTG	1 cut(s)	2960
PpsI	GAGTC	5 cut(s)	125, 310, 430, 703, 2666
Ppu21I	YACGTR	2 cut(s)	2009, 2960
PpuMI	RGGWCCY	3 cut(s)	469, 2313, 2969
PscI	ACATGT	1 cut(s)	1060
PshAI	GACNNNNGTC	1 cut(s)	2318
PshBI	ATTAAT	2 cut(s)	576, 2175
PsiI	TTATAA	2 cut(s)	1910, 2211
Psp124BI	GAGCTC	1 cut(s)	752
Psp5II	RGGWCCY	3 cut(s)	469, 2313, 2969
Psp6I	CCWGG	4 cut(s)	924, 1410, 2354, 2493
PspCI	CACGTG	1 cut(s)	2960
PspEI	GGTNACC	1 cut(s)	224
PspFI	CCCAGC	5 cut(s)	535, 713, 995, 1388, 1725
PspGI	CCWGG	4 cut(s)	924, 1410, 2354, 2493
PspN4I	GGNNCC	9 cut(s)	369, 471, 1002, 1204, 2314, 2315, 2353, 2970, 3331
PspPI	GGNCC	6 cut(s)	469, 1202, 2313, 2491, 2906, 2969
PspPPI	RGGWCCY	3 cut(s)	469, 2313, 2969
PstNI	CAGNNNCTG	1 cut(s)	127
PsuI	RGATCY	3 cut(s)	1192, 2949, 2979
PsyI	GACNNNGTC	1 cut(s)	301
PvuII	CAGCTG	5 cut(s)	127, 995, 1388, 1619, 2168
RsaI	GTAC	8 cut(s)	20, 32, 499, 681, 1002, 1657, 2102, 2976
RsaNI	GTAC	8 cut(s)	19, 31, 498, 680, 1001, 1656, 2101, 2975
RseI	CAYNNNNRTG	8 cut(s)	417, 452, 630, 1065, 1607, 2826, 2958, 3181
SacI	GAGCTC	1 cut(s)	752
SalI	GTCGAC	1 cut(s)	173
SapI	GCTCTTC	3 cut(s)	3, 18, 2823
SaqAI	TTAA	9 cut(s)	576, 1257, 1317, 1418, 1716, 1769, 2175, 3084, 3146
Sau96I	GGNCC	6 cut(s)	469, 1202, 2313, 2491, 2906, 2969
SchI	GAGTC	5 cut(s)	125, 311, 430, 704, 2667
ScrFI	CCNGG	9 cut(s)	221, 222, 742, 833, 926, 1412, 1504, 2356, 2495
SduI	GDGCHC	5 cut(s)	620, 752, 838, 873, 1141
SexAI	ACCWGGT	1 cut(s)	2493
SfaNI	GCATC	4 cut(s)	1445, 2152, 2761, 3100
SfcI	CTRYAG	5 cut(s)	2247, 2287, 2649, 3115, 3273
SfoI	GGCGCC	1 cut(s)	369
SfuI	TTCGAA	2 cut(s)	99, 2557
SinI	GGWCC	4 cut(s)	469, 2313, 2491, 2969
SmaI	CCCGGG	1 cut(s)	222
SmiMI	CAYNNNNRTG	8 cut(s)	417, 452, 630, 1065, 1607, 2826, 2958, 3181
SmlI	CTYRAG	1 cut(s)	3164
SmoI	CTYRAG	1 cut(s)	3164
SphI	GCATGC	1 cut(s)	1569
SspDI	GGCGCC	1 cut(s)	367
SspI	AATATT	1 cut(s)	1349
SspMI	CTAG	3 cut(s)	965, 1005, 1190
SstI	GAGCTC	1 cut(s)	752
StyD4I	CCNGG	9 cut(s)	219, 220, 740, 831, 924, 1410, 1502, 2354, 2493
StyI	CCWWGG	7 cut(s)	556, 1248, 2028, 2136, 2642, 2902, 3267
TaiI	ACGT	5 cut(s)	630, 2011, 2341, 2449, 2962
TaqI	TCGA	7 cut(s)	99, 138, 174, 192, 488, 2557, 3180
TaqII	GACCGA	1 cut(s)	257
TatI	WGTACW	4 cut(s)	30, 497, 2100, 2974
TauI	GCSGC	3 cut(s)	185, 1786, 2119
TfiI	GAWTC	5 cut(s)	206, 811, 949, 2222, 2236
Tru1I	TTAA	9 cut(s)	576, 1257, 1317, 1418, 1716, 1769, 2175, 3084, 3146
Tru9I	TTAA	9 cut(s)	576, 1257, 1317, 1418, 1716, 1769, 2175, 3084, 3146
TscAI	CASTG	8 cut(s)	330, 1342, 1529, 2091, 2277, 2479, 2616, 2878
TseFI	GTSAC	9 cut(s)	224, 385, 2056, 2122, 2268, 2447, 2659, 2791, 2867
Tsp45I	GTSAC	9 cut(s)	224, 385, 2056, 2122, 2268, 2447, 2659, 2791, 2867
TspDTI	ATGAA	8 cut(s)	136, 448, 559, 1873, 1916, 2249, 3113, 3165
TspGWI	ACGGA	4 cut(s)	473, 545, 783, 1817
TspMI	CCCGGG	1 cut(s)	220
TspRI	CASTG	8 cut(s)	330, 1342, 1529, 2091, 2277, 2479, 2616, 2878
Tth111I	GACNNNGTC	1 cut(s)	301
Van91I	CCANNNNNTGG	2 cut(s)	1064, 2570
VneI	GTGCAC	1 cut(s)	1137
VpaK11BI	GGWCC	4 cut(s)	469, 2313, 2491, 2969
VspI	ATTAAT	2 cut(s)	576, 2175
XapI	RAATTY	9 cut(s)	1465, 1637, 1663, 1674, 1977, 2049, 2559, 2579, 3172
XbaI	TCTAGA	1 cut(s)	964
XceI	RCATGY	3 cut(s)	1064, 1569, 2641
XmaI	CCCGGG	1 cut(s)	220
XmiI	GTMKAC	2 cut(s)	174, 1599
XmnI	GAANNNNTTC	2 cut(s)	1053, 3176
XspI	CTAG	3 cut(s)	965, 1005, 1190
ZraI	GACGTC	1 cut(s)	2339
Zsp2I	ATGCAT	2 cut(s)	1438, 1933

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.