AT4G22860

Protein TPX2-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005322
Physical Location & Seq
Forward (+)
11997281 .. 12001631
4351 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22860.2

Sequence Viewer

Length: 1530 bp
ATGGAGATGGATGAAGATATGGAAATCGAGCCAATGGTGTTTGAGGTTAAAGAGATCGATCTGGAGTACGAATTTGATGCGGCGAGGTGGTACGATTTTACTCGGGAAGAGTTACCGGAGGAGTCTCAAGCCGCTGAGTTCTGGTTTCACTCTGCTCCGTCGTACGCTCCTTCTCCTTTTGTAACAAAACTGTTACTAAGAGAGGAGGTTTATGATGACAAGACGGAGGCATCTACGAGATCAGAGGATGAGGAAGTCGTTGCAGATGTTTGTGATAAGTATAAAGGAACCTTTCAGCAACCAAATCATTTAGCCACAGATTTTAATAAGACAGGAAGTAGAATGCGTTCTGGGATTTTTTCCTCCCAACAACAACAAGGTTGTAATTTGAAGAAAGTTCCAAATCAGCCCATATGGAAAGGGCCGACGGTTAGCAATCACAAACACACTGATAAACCAAAATTCCGAGCTAAGTCAAGCATCAGACCAACTCCAAGGAGCTCAACATTAATGAGACCTACTGCTAGCCAATTAGCAAAACAAAATAATGCCAGTAAATTCCATATGCAAGTGGATCAAAGTCATGATAAAGGAAGTTGTGGGACATCTGGGACTGAAGTTCAAGCGTCTAAAAGACAGAAGCTTGATGGAGGTCTTCTTCGTAAGGTTGCTGATACAAAGCAGGAAATGAGTTTTGTCCACAAGATCCCCAAGAAGGATACAACTCTTGATAAAAACGCTCAACATGCCAGGACAAAGATTACTATCCCACAGGAGCCTGATTTTGCTACATCACAGAGGGCTCACAGGATACGGCACAAGAATGATGCGAAGTTAGAACAAGAGCAACCAGCTGTATATAGATTCAAAGCACGTCCATTTAACCGAAAGATATTTGATGCTCCTTCATTGCCCATCCGGAAAAAGAGCACTCCGAAACTACCAGAATTCCAAGAATTTCATTTGAAGACTTCAGAAAGGGCTATGCAACATTCATCAGCAGTAACAACACGGTCTCATGCGTATAAGGAGTCAGACAAATCTATTATAACTGATATGCTTGACGGTGATAACAGAGAAAGTAGGAGACCAAGCGGTATGGATATATCTAAGCATGATGTCTCAGAAGGAAGTCACGTTTTTAAAGCTCGTCCTCTGAACAAGAAGATACTTTCAAGCAGAGGAGACATGGGCATTTTCAAAAATAGCAAGCGAGAAACAACAGTACCTTTGGAGTTTAGTTTTCATTCGGAAAAGAGAGTTCAACCAGATTTACCGACAGACCTTTTCAGCAAGCTCTCCATAAAATCCGAGATACAGCCAAACGATGGATCTCGCACAAGATTATTTCAACCAAAGGTCTCAAAGGAAAATAGAATAAACTCTTTCCAAGCAAGGAATGAGGTAACAAGAATGGCAGCGGCAAAACCAGTCTCTTCAGCTGGACAACAAATGCAATTTGGTAATAGTGGAATAACTCCTGAAACAAACCAGCAATGGACAGCTAGGAGGAACTTAGGCATTCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2_importin

Protein Analysis

509

Amino Acids

58.16

Weight (kDa)

9.48

Isoelectric Point (pI)

49.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2_importin PF12214 245 - 407 1.1e-51 Cell cycle regulated microtubule associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1049
AccB7I CCANNNNNTGG 1 cut(s) 1328
AccIII TCCGGA 1 cut(s) 918
AciI CCGC 4 cut(s) 80, 132, 1095, 1421
AclWI GGATC 3 cut(s) 582, 700, 1339
AcsI RAATTY 5 cut(s) 71, 461, 557, 947, 956
AcuI CTGAAG 3 cut(s) 636, 957, 1422
AfaI GTAC 4 cut(s) 68, 92, 164, 1227
AfiI CCNNNNNNNGG 2 cut(s) 715, 1328
AgsI TTSAA 8 cut(s) 391, 623, 868, 967, 1176, 1201, 1265, 1352
AjiI CACGTC 1 cut(s) 875
AjnI CCWGG 1 cut(s) 749
AluBI AGCT 8 cut(s) 470, 501, 643, 854, 1148, 1297, 1442, 1505
AluI AGCT 8 cut(s) 470, 501, 643, 854, 1148, 1297, 1442, 1505
Alw21I GWGCWC 2 cut(s) 503, 932
Alw26I GTCTC 8 cut(s) 129, 508, 1020, 1081, 1126, 1179, 1366, 1438
AlwI GGATC 3 cut(s) 582, 700, 1339
Ama87I CYCGRG 1 cut(s) 102
Aor13HI TCCGGA 1 cut(s) 918
AoxI GGCC 1 cut(s) 422
ApeKI GCWGC 1 cut(s) 1418
ApoI RAATTY 5 cut(s) 71, 461, 557, 947, 956
AseI ATTAAT 1 cut(s) 509
Asp700I GAANNNNTTC 1 cut(s) 346
AspS9I GGNCC 1 cut(s) 422
AsuHPI GGTGA 1 cut(s) 1079
AsuNHI GCTAGC 1 cut(s) 524
AvaI CYCGRG 1 cut(s) 102
BaeI ACNNNNGTAYC 2 cut(s) 1209, 1242
BanII GRGCYC 2 cut(s) 503, 805
BbsI GAAGAC 2 cut(s) 647, 974
Bbv12I GWGCWC 2 cut(s) 503, 932
BbvI GCAGC 1 cut(s) 1430
BccI CCATC 3 cut(s) 641, 923, 1322
BceAI ACGGC 1 cut(s) 830
BcgI CGANNNNNNTGC 2 cut(s) 59, 93
BciT130I CCWGG 1 cut(s) 751
BciVI GTATCC 2 cut(s) 712, 804
BcoDI GTCTC 8 cut(s) 129, 508, 1020, 1081, 1126, 1179, 1366, 1438
BfaI CTAG 2 cut(s) 525, 1506
BfuI GTATCC 2 cut(s) 712, 804
BisI GCNGC 4 cut(s) 81, 132, 1419, 1422
BlsI GCNGC 4 cut(s) 82, 133, 1420, 1423
Bme1390I CCNGG 1 cut(s) 751
BmeT110I CYCGRG 1 cut(s) 102
BmgBI CACGTC 1 cut(s) 875
BmgT120I GGNCC 1 cut(s) 422
BmiI GGNNCC 2 cut(s) 289, 777
BmrFI CCNGG 1 cut(s) 751
BmsI GCATC 5 cut(s) 67, 239, 489, 817, 889
BmtI GCTAGC 1 cut(s) 528
BpiI GAAGAC 2 cut(s) 647, 974
BpmI CTGGAG 1 cut(s) 83
BpuEI CTTGAG 1 cut(s) 111
Bsa29I ATCGAT 1 cut(s) 57
BsaBI GATNNNNATC 1 cut(s) 764
BsaI GGTCTC 4 cut(s) 508, 1020, 1081, 1366
BsaJI CCNNGG 1 cut(s) 494
BsaWI WCCGGW 2 cut(s) 115, 918
Bsc4I CCNNNNNNNGG 2 cut(s) 715, 1328
Bse1I ACTGG 2 cut(s) 552, 1430
Bse3DI GCAATG 2 cut(s) 908, 1502
Bse8I GATNNNNATC 1 cut(s) 764
BseAI TCCGGA 1 cut(s) 918
BseBI CCWGG 1 cut(s) 751
BseCI ATCGAT 1 cut(s) 57
BseDI CCNNGG 1 cut(s) 494
BseGI GGATG 3 cut(s) 16, 253, 915
BseJI GATNNNNATC 1 cut(s) 764
BseLI CCNNNNNNNGG 2 cut(s) 715, 1328
BseMI GCAATG 2 cut(s) 908, 1502
BseMII CTCAG 2 cut(s) 126, 1137
BseNI ACTGG 2 cut(s) 552, 1430
BseRI GAGGAG 3 cut(s) 134, 218, 1197
BseXI GCAGC 1 cut(s) 1430
BshFI GGCC 1 cut(s) 424
BshVI ATCGAT 1 cut(s) 57
BsiHKAI GWGCWC 2 cut(s) 503, 932
BsiHKCI CYCGRG 1 cut(s) 102
BsiSI CCGG 2 cut(s) 116, 919
BsiWI CGTACG 1 cut(s) 162
BslFI GGGAC 2 cut(s) 616, 625
BslI CCNNNNNNNGG 2 cut(s) 715, 1328
BsmAI GTCTC 8 cut(s) 129, 508, 1020, 1081, 1126, 1179, 1366, 1438
BsmFI GGGAC 2 cut(s) 616, 625
BsmI GAATGC 2 cut(s) 348, 1521
BsnI GGCC 1 cut(s) 424
Bso31I GGTCTC 4 cut(s) 508, 1020, 1081, 1366
BsoBI CYCGRG 1 cut(s) 102
Bsp1286I GDGCHC 3 cut(s) 503, 805, 932
Bsp13I TCCGGA 1 cut(s) 918
Bsp143I GATC 6 cut(s) 54, 58, 239, 574, 705, 1331
BspACI CCGC 4 cut(s) 80, 132, 1095, 1421
BspANI GGCC 1 cut(s) 424
BspCNI CTCAG 2 cut(s) 127, 1136
BspDI ATCGAT 1 cut(s) 57
BspEI TCCGGA 1 cut(s) 918
BspHI TCATGA 1 cut(s) 583
BspLI GGNNCC 2 cut(s) 289, 777
BspOI GCTAGC 1 cut(s) 528
BspPI GGATC 3 cut(s) 582, 700, 1339
BspTNI GGTCTC 4 cut(s) 508, 1020, 1081, 1366
BsrDI GCAATG 2 cut(s) 908, 1502
BsrI ACTGG 2 cut(s) 552, 1430
BssECI CCNNGG 1 cut(s) 494
BssMI GATC 6 cut(s) 54, 58, 239, 574, 705, 1331
BssT1I CCWWGG 1 cut(s) 494
Bst2UI CCWGG 1 cut(s) 751
Bst4CI ACNGT 5 cut(s) 192, 430, 1014, 1067, 1225
Bst6I CTCTTC 2 cut(s) 102, 1441
BstC8I GCNNGC 3 cut(s) 526, 1211, 1295
BstDEI CTNAG 6 cut(s) 135, 197, 471, 1110, 1123, 1516
BstF5I GGATG 3 cut(s) 16, 253, 915
BstKTI GATC 6 cut(s) 57, 61, 242, 577, 708, 1334
BstMAI GTCTC 8 cut(s) 129, 508, 1020, 1081, 1126, 1179, 1366, 1438
BstMBI GATC 6 cut(s) 54, 58, 239, 574, 705, 1331
BstMWI GCNNNNNNNGC 1 cut(s) 746
BstNI CCWGG 1 cut(s) 751
BstNSI RCATGY 1 cut(s) 749
BstSCI CCNGG 1 cut(s) 749
BstV1I GCAGC 1 cut(s) 1430
BstV2I GAAGAC 2 cut(s) 647, 974
BstX2I RGATCY 2 cut(s) 705, 1331
BstYI RGATCY 2 cut(s) 705, 1331
Bsu15I ATCGAT 1 cut(s) 57
BsuI GTATCC 2 cut(s) 712, 804
BsuRI GGCC 1 cut(s) 424
BsuTUI ATCGAT 1 cut(s) 57
BtrI CACGTC 1 cut(s) 875
BtsCI GGATG 3 cut(s) 16, 253, 915
BtsIMutI CAGTG 1 cut(s) 447
Cac8I GCNNGC 3 cut(s) 526, 1211, 1295
CciI TCATGA 1 cut(s) 583
Cfr13I GGNCC 1 cut(s) 422
ClaI ATCGAT 1 cut(s) 57
CseI GACGC 1 cut(s) 615
Csp6I GTAC 4 cut(s) 67, 91, 163, 1226
CviAII CATG 5 cut(s) 584, 746, 1019, 1115, 1189
CviQI GTAC 4 cut(s) 67, 91, 163, 1226
DdeI CTNAG 6 cut(s) 135, 197, 471, 1110, 1123, 1516
DpnI GATC 6 cut(s) 56, 60, 241, 576, 707, 1333
DpnII GATC 6 cut(s) 54, 58, 239, 574, 705, 1331
DraI TTTAAA 1 cut(s) 1144
Eam1104I CTCTTC 2 cut(s) 102, 1441
EarI CTCTTC 2 cut(s) 102, 1441
Ecl136II GAGCTC 1 cut(s) 501
Eco130I CCWWGG 1 cut(s) 494
Eco24I GRGCYC 2 cut(s) 503, 805
Eco31I GGTCTC 4 cut(s) 508, 1020, 1081, 1366
Eco53kI GAGCTC 1 cut(s) 501
Eco57I CTGAAG 3 cut(s) 636, 957, 1422
Eco88I CYCGRG 1 cut(s) 102
EcoICRI GAGCTC 1 cut(s) 501
EcoRI GAATTC 1 cut(s) 947
EcoRII CCWGG 1 cut(s) 749
EcoT14I CCWWGG 1 cut(s) 494
EcoT38I GRGCYC 2 cut(s) 503, 805
ErhI CCWWGG 1 cut(s) 494
FaeI CATG 5 cut(s) 587, 749, 1022, 1118, 1192
FaqI GGGAC 2 cut(s) 616, 625
FatI CATG 5 cut(s) 583, 745, 1018, 1114, 1188
FauNDI CATATG 2 cut(s) 413, 564
Fnu4HI GCNGC 4 cut(s) 81, 132, 1419, 1422
FokI GGATG 3 cut(s) 23, 260, 902
FriOI GRGCYC 2 cut(s) 503, 805
Fsp4HI GCNGC 4 cut(s) 81, 132, 1419, 1422
FspBI CTAG 2 cut(s) 525, 1506
GluI GCNGC 4 cut(s) 81, 132, 1419, 1422
GsuI CTGGAG 1 cut(s) 83
HaeIII GGCC 1 cut(s) 424
HapII CCGG 2 cut(s) 116, 919
HgaI GACGC 1 cut(s) 615
Hin1II CATG 5 cut(s) 587, 749, 1022, 1118, 1192
HindIII AAGCTT 1 cut(s) 641
HinfI GANTC 3 cut(s) 122, 864, 1031
HpaII CCGG 2 cut(s) 116, 919
HphI GGTGA 1 cut(s) 1079
Hpy166II GTNNAC 1 cut(s) 700
Hpy188III TCNNGA 6 cut(s) 62, 104, 584, 728, 919, 1481
Hpy8I GTNNAC 1 cut(s) 700
Hpy99I CGWCG 2 cut(s) 163, 430
HpyAV CCTTC 4 cut(s) 180, 709, 915, 1121
HpyCH4III ACNGT 5 cut(s) 192, 430, 1014, 1067, 1225
HpyCH4IV ACGT 2 cut(s) 874, 1137
HpyCH4V TGCA 4 cut(s) 263, 568, 988, 1456
HpyF10VI GCNNNNNNNGC 1 cut(s) 746
HpyF3I CTNAG 6 cut(s) 135, 197, 471, 1110, 1123, 1516
HpySE526I ACGT 2 cut(s) 874, 1137
Hsp92II CATG 5 cut(s) 587, 749, 1022, 1118, 1192
Kpn2I TCCGGA 1 cut(s) 918
Kzo9I GATC 6 cut(s) 54, 58, 239, 574, 705, 1331
LmnI GCTCC 5 cut(s) 160, 172, 498, 775, 907
Lsp1109I GCAGC 1 cut(s) 1430
LweI GCATC 5 cut(s) 67, 239, 489, 817, 889
MaeI CTAG 2 cut(s) 525, 1506
MaeII ACGT 2 cut(s) 874, 1137
MaeIII GTNAC 6 cut(s) 111, 181, 192, 1003, 1133, 1405
MalI GATC 6 cut(s) 56, 60, 241, 576, 707, 1333
MboI GATC 6 cut(s) 54, 58, 239, 574, 705, 1331
MboII GAAGA 8 cut(s) 26, 119, 403, 647, 650, 979, 1177, 1428
MflI RGATCY 2 cut(s) 705, 1331
MhlI GDGCHC 3 cut(s) 503, 805, 932
MluCI AATT 8 cut(s) 71, 385, 461, 530, 557, 947, 956, 1457
MlyI GAGTC 2 cut(s) 131, 1040
MroI TCCGGA 1 cut(s) 918
MroXI GAANNNNTTC 1 cut(s) 346
MseI TTAA 5 cut(s) 48, 324, 509, 882, 1143
MslI CAYNNNNRTG 1 cut(s) 822
MspA1I CMGCKG 4 cut(s) 134, 854, 1421, 1442
MspI CCGG 2 cut(s) 116, 919
MspR9I CCNGG 1 cut(s) 751
Mva1269I GAATGC 2 cut(s) 348, 1521
MvaI CCWGG 1 cut(s) 751
MwoI GCNNNNNNNGC 1 cut(s) 746
NdeI CATATG 2 cut(s) 413, 564
NdeII GATC 6 cut(s) 54, 58, 239, 574, 705, 1331
NheI GCTAGC 1 cut(s) 524
NlaIII CATG 5 cut(s) 587, 749, 1022, 1118, 1192
NlaIV GGNNCC 2 cut(s) 289, 777
NmuCI GTSAC 1 cut(s) 1133
NspI RCATGY 1 cut(s) 749
PagI TCATGA 1 cut(s) 583
PctI GAATGC 2 cut(s) 348, 1521
PdmI GAANNNNTTC 1 cut(s) 346
PfeI GAWTC 1 cut(s) 864
Pfl23II CGTACG 1 cut(s) 162
PflMI CCANNNNNTGG 1 cut(s) 1328
PkrI GCNGC 4 cut(s) 82, 133, 1420, 1423
PleI GAGTC 2 cut(s) 130, 1039
PpsI GAGTC 2 cut(s) 130, 1039
PshBI ATTAAT 1 cut(s) 509
PsiI TTATAA 1 cut(s) 1049
Psp124BI GAGCTC 1 cut(s) 503
Psp6I CCWGG 1 cut(s) 749
PspGI CCWGG 1 cut(s) 749
PspLI CGTACG 1 cut(s) 162
PspN4I GGNNCC 2 cut(s) 289, 777
PspPI GGNCC 1 cut(s) 422
PsrI GAACNNNNNNTAC 2 cut(s) 331, 363
PsuI RGATCY 2 cut(s) 705, 1331
PvuII CAGCTG 2 cut(s) 854, 1442
RsaI GTAC 4 cut(s) 68, 92, 164, 1227
RsaNI GTAC 4 cut(s) 67, 91, 163, 1226
RseI CAYNNNNRTG 1 cut(s) 822
SacI GAGCTC 1 cut(s) 503
SaqAI TTAA 5 cut(s) 48, 324, 509, 882, 1143
SatI GCNGC 4 cut(s) 81, 132, 1419, 1422
Sau3AI GATC 6 cut(s) 54, 58, 239, 574, 705, 1331
Sau96I GGNCC 1 cut(s) 422
SchI GAGTC 2 cut(s) 131, 1040
ScrFI CCNGG 1 cut(s) 751
SduI GDGCHC 3 cut(s) 503, 805, 932
SfaNI GCATC 5 cut(s) 67, 239, 489, 817, 889
SmiMI CAYNNNNRTG 1 cut(s) 822
SmlI CTYRAG 1 cut(s) 126
SmoI CTYRAG 1 cut(s) 126
Sse9I AATT 8 cut(s) 71, 385, 461, 530, 557, 947, 956, 1457
SsiI CCGC 4 cut(s) 80, 132, 1095, 1421
SspMI CTAG 2 cut(s) 525, 1506
SstI GAGCTC 1 cut(s) 503
StyD4I CCNGG 1 cut(s) 749
StyI CCWWGG 1 cut(s) 494
TaaI ACNGT 5 cut(s) 192, 430, 1014, 1067, 1225
TaiI ACGT 2 cut(s) 877, 1140
TaqI TCGA 2 cut(s) 27, 57
TasI AATT 8 cut(s) 71, 385, 461, 530, 557, 947, 956, 1457
TauI GCSGC 3 cut(s) 83, 134, 1424
TfiI GAWTC 1 cut(s) 864
Tru1I TTAA 5 cut(s) 48, 324, 509, 882, 1143
Tru9I TTAA 5 cut(s) 48, 324, 509, 882, 1143
TscAI CASTG 1 cut(s) 454
TseFI GTSAC 1 cut(s) 1133
TseI GCWGC 1 cut(s) 1418
Tsp45I GTSAC 1 cut(s) 1133
TspDTI ATGAA 5 cut(s) 27, 897, 950, 984, 1235
TspGWI ACGGA 2 cut(s) 147, 239
TspRI CASTG 1 cut(s) 454
Van91I CCANNNNNTGG 1 cut(s) 1328
VspI ATTAAT 1 cut(s) 509
XapI RAATTY 5 cut(s) 71, 461, 557, 947, 956
XceI RCATGY 1 cut(s) 749
XmnI GAANNNNTTC 1 cut(s) 346
XspI CTAG 2 cut(s) 525, 1506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.