FvH4_5g00990

Protein TPX2-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005322
Physical Location & Seq
Forward (+)
583380 .. 588589
5210 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00990.t6

Sequence Viewer

Length: 1521 bp
ATGGAGGAGGATATGGAGGTGGAGGAGGAGCAAGTGTTTACGGCGCACGAGGTGGACATTGACTACGAGTTTGACGCGGCGAGGTTCTTTGATTTCAGCCGGGAGGAGACGCCGGTGGAGGCCCGCCGTGCTGAGCTCTGGTTCGAGTCCGCCAAGACCTATCCTCCGTCTCCTGTAGTGACAAAGTTGTTACTAGGAGGGGACATTCTCCTGGGAAATGTGAACACCTCTCCGAAATCATCATCGAAAGATTTGGAGAGTACAAATGGCGTTGGATGTAATACCGGTACGGGCCAAGAATCGTGTGCAATGGATGTAAATGGCAGAGATTGTGGAGGGACGAATACAGGGATCTTTGGAAATTTACAAAGAGTTCTAAATCAACCGCATGGAGCGGGTACAGGGTTGATATTCAATCATTCAAAAGGTCAACATAACTCTTCAGTAAAGCCATCTTTCCCCAGGAAATCGACTTTGATGAAACCTACAGCAAGTCAACTTGCTAAGCAAAATCGCCCTCGTCAGATGGGTGGTTCCAGATTTCAGACACTACATGTTCAAAACAGTGAGAAGAGCTTATGTAGTTCTTCTGGAGTTGAAACTCAAGCTACCAAAAGGCAGAAGTTAGAGGGAGGTCACTTGCATAAGGTTACTACTGATGCAATGCAGCAAACTAATTTCATCCACAAGGTTGAAAATTTTGATAAGACCTCTACACATGCCAAGTTGAAACTTACTATTCCAATAGAGCCTGACTTTGAAACGACATATAGGGCGCAAAGAATAAGGCCTAAGAATGGATTAGTGTCGGAACAAGTGCCACCAGCTCCTCGAAAATTCAAAGCCCGTCCATTGAACAAAAAAATTTTTGAGGCTCCTTCACTGCCACTCTCAAAGAGGAGCACTCCAAAGTTGCCAGAATTTCATGAATTTCACCTGAAGACAATGGAAAGAGCTATGCAACATACATCTACTGTTCCATCATCCTCACACCATTTCAATGGTCAAGACAAGGTGTTGGACAAACCTAGTGCTTCCACTTTTGCAGGAAATGGAAATGGAGAACCCAGAAGGCCAAGTAAATTGGATGGTCCTAAGCAGGATGGAAGTGATGTAATACCAAAATTTAAAGCTCGTCCTCTTAATAAAAAGATCTTTTCTAGCAAAGGGGATATTGGTGTTTTCTGGAATAGCAAAAGAGAAACCACTGTACCAATGGAATTTAATTTTCAAACAGAGAGGAGAACTCAGCAGATTCCGCCAACTGATCTTTTCAGTAAGCTCTCGTTAACATCTGAACTCCAGCCGAATAATGGATCTCGGTTTAAATTACCTCAGCCAGCATCTGTATCAATGAAGGACTCAAAAGAAAATAGAGCGAATAATTTCCAACCAGAAAATAAGATAATGGTCAAAGAGAAACCTTCTTTGTTTGGTGGCAAGCAGGCTTTGTTTGGAGGTGCCAGGCCCGTCAGTGATGTGGATAACAAATCAAGGATGAGGAGTCTGGGAGTCCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2_importin

Protein Analysis

507

Amino Acids

56.31

Weight (kDa)

9.61

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2_importin PF12214 247 - 414 2.8e-45 Cell cycle regulated microtubule associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1460
AccBSI CCGCTC 1 cut(s) 395
AccII CGCG 1 cut(s) 77
AciI CCGC 7 cut(s) 77, 124, 150, 386, 395, 1259, 1516
AclWI GGATC 2 cut(s) 359, 1324
AcsI RAATTY 8 cut(s) 361, 697, 836, 864, 920, 929, 1124, 1220
AcuI CTGAAG 2 cut(s) 426, 959
AcyI GRCGYC 1 cut(s) 110
AdeI CACNNNGTG 1 cut(s) 52
AfaI GTAC 4 cut(s) 262, 289, 400, 1212
AfiI CCNNNNNNNGG 2 cut(s) 797, 1313
AflIII ACRYGT 1 cut(s) 553
AgeI ACCGGT 1 cut(s) 284
AjnI CCWGG 3 cut(s) 210, 461, 1463
AluBI AGCT 7 cut(s) 136, 576, 608, 827, 956, 1133, 1282
AluI AGCT 7 cut(s) 136, 576, 608, 827, 956, 1133, 1282
Alw21I GWGCWC 2 cut(s) 138, 905
Alw26I GTCTC 2 cut(s) 101, 174
AlwI GGATC 2 cut(s) 359, 1324
AlwNI CAGNNNCTG 1 cut(s) 1346
AoxI GGCC 5 cut(s) 120, 292, 788, 1073, 1466
ApeKI GCWGC 1 cut(s) 667
ApoI RAATTY 8 cut(s) 361, 697, 836, 864, 920, 929, 1124, 1220
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
AsiGI ACCGGT 1 cut(s) 284
Asp700I GAANNNNTTC 1 cut(s) 1385
AspLEI GCGC 2 cut(s) 46, 778
AspS9I GGNCC 4 cut(s) 121, 292, 1091, 1467
AsuC2I CCSGG 1 cut(s) 101
AsuHPI GGTGA 1 cut(s) 926
AvaII GGWCC 1 cut(s) 1091
BaeI ACNNNNGTAYC 2 cut(s) 1194, 1227
BanI GGYRCC 1 cut(s) 1460
BanII GRGCYC 1 cut(s) 138
BauI CACGAG 1 cut(s) 47
BbsI GAAGAC 1 cut(s) 947
Bbv12I GWGCWC 2 cut(s) 138, 905
BbvCI CCTCAGC 1 cut(s) 1335
BbvI GCAGC 1 cut(s) 679
BccI CCATC 5 cut(s) 460, 520, 988, 1082, 1097
BceAI ACGGC 2 cut(s) 57, 111
BciT130I CCWGG 3 cut(s) 212, 463, 1465
BcnI CCSGG 1 cut(s) 101
BcoDI GTCTC 2 cut(s) 101, 174
BfaI CTAG 4 cut(s) 194, 1029, 1161, 1519
BfmI CTRYAG 2 cut(s) 174, 486
BglII AGATCT 1 cut(s) 1152
BisI GCNGC 2 cut(s) 78, 668
BlpI GCTNAGC 2 cut(s) 132, 504
BlsI GCNGC 2 cut(s) 79, 669
Bme1390I CCNGG 4 cut(s) 101, 212, 463, 1465
Bme18I GGWCC 1 cut(s) 1091
BmgT120I GGNCC 4 cut(s) 121, 292, 1091, 1467
BmiI GGNNCC 3 cut(s) 535, 876, 1462
BmrFI CCNGG 4 cut(s) 101, 212, 463, 1465
BmsI GCATC 2 cut(s) 649, 1352
BpiI GAAGAC 1 cut(s) 947
BplI GAGNNNNNCTC 4 cut(s) 889, 921, 1231, 1263
BpmI CTGGAG 2 cut(s) 612, 1286
Bpu10I CCTNAGC 2 cut(s) 1095, 1335
Bpu1102I GCTNAGC 2 cut(s) 132, 504
BpuEI CTTGAG 1 cut(s) 588
BpuMI CCSGG 1 cut(s) 101
BsaHI GRCGYC 1 cut(s) 110
BsaJI CCNNGG 2 cut(s) 211, 461
BsaWI WCCGGW 1 cut(s) 284
BsaXI ACNNNNNCTCC 4 cut(s) 20, 50, 148, 178
Bsc4I CCNNNNNNNGG 2 cut(s) 797, 1313
Bse118I RCCGGY 2 cut(s) 112, 284
Bse3DI GCAATG 2 cut(s) 315, 669
BseBI CCWGG 3 cut(s) 212, 463, 1465
BseDI CCNNGG 2 cut(s) 211, 461
BseGI GGATG 7 cut(s) 281, 319, 681, 983, 1093, 1108, 1503
BseLI CCNNNNNNNGG 2 cut(s) 797, 1313
BseMI GCAATG 2 cut(s) 315, 669
BseMII CTCAG 3 cut(s) 123, 1262, 1349
BseRI GAGGAG 8 cut(s) 20, 38, 41, 119, 819, 913, 1255, 1516
BseXI GCAGC 1 cut(s) 679
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 5 cut(s) 122, 294, 790, 1075, 1468
BshNI GGYRCC 1 cut(s) 1460
BshTI ACCGGT 1 cut(s) 284
BsiHKAI GWGCWC 2 cut(s) 138, 905
BsiSI CCGG 3 cut(s) 100, 113, 285
BslFI GGGAC 2 cut(s) 215, 352
BslI CCNNNNNNNGG 2 cut(s) 797, 1313
BsmAI GTCTC 2 cut(s) 101, 174
BsmBI CGTCTC 2 cut(s) 101, 174
BsmFI GGGAC 2 cut(s) 215, 352
BsnI GGCC 5 cut(s) 122, 294, 790, 1075, 1468
Bsp1286I GDGCHC 2 cut(s) 138, 905
Bsp143I GATC 4 cut(s) 351, 1152, 1267, 1316
Bsp1720I GCTNAGC 2 cut(s) 132, 504
BspACI CCGC 7 cut(s) 77, 124, 150, 386, 395, 1259, 1516
BspANI GGCC 5 cut(s) 122, 294, 790, 1075, 1468
BspCNI CTCAG 3 cut(s) 124, 1261, 1348
BspFNI CGCG 1 cut(s) 77
BspHI TCATGA 1 cut(s) 925
BspLI GGNNCC 3 cut(s) 535, 876, 1462
BspPI GGATC 2 cut(s) 359, 1324
BspQI GCTCTTC 1 cut(s) 566
BspT107I GGYRCC 1 cut(s) 1460
BsrBI CCGCTC 1 cut(s) 395
BsrDI GCAATG 2 cut(s) 315, 669
BsrFI RCCGGY 2 cut(s) 112, 284
BssAI RCCGGY 2 cut(s) 112, 284
BssECI CCNNGG 2 cut(s) 211, 461
BssMI GATC 4 cut(s) 351, 1152, 1267, 1316
BssNI GRCGYC 1 cut(s) 110
BssSI CACGAG 1 cut(s) 47
Bst2BI CACGAG 1 cut(s) 47
Bst2UI CCWGG 3 cut(s) 212, 463, 1465
Bst4CI ACNGT 3 cut(s) 566, 976, 1210
Bst6I CTCTTC 2 cut(s) 445, 566
BstACI GRCGYC 1 cut(s) 110
BstC8I GCNNGC 4 cut(s) 124, 1341, 1442, 1446
BstDEI CTNAG 6 cut(s) 132, 504, 792, 1095, 1248, 1335
BstF5I GGATG 7 cut(s) 281, 319, 681, 983, 1093, 1108, 1503
BstFNI CGCG 1 cut(s) 77
BstHHI GCGC 2 cut(s) 46, 778
BstKTI GATC 4 cut(s) 354, 1155, 1270, 1319
BstMAI GTCTC 2 cut(s) 101, 174
BstMBI GATC 4 cut(s) 351, 1152, 1267, 1316
BstMWI GCNNNNNNNGC 2 cut(s) 128, 1258
BstNI CCWGG 3 cut(s) 212, 463, 1465
BstNSI RCATGY 2 cut(s) 557, 722
BstSCI CCNGG 4 cut(s) 99, 210, 461, 1463
BstSFI CTRYAG 2 cut(s) 174, 486
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 1 cut(s) 679
BstV2I GAAGAC 1 cut(s) 947
BstX2I RGATCY 3 cut(s) 351, 1152, 1316
BstXI CCANNNNNNTGG 1 cut(s) 1001
BstYI RGATCY 3 cut(s) 351, 1152, 1316
BsuRI GGCC 5 cut(s) 122, 294, 790, 1075, 1468
BtsCI GGATG 7 cut(s) 281, 319, 681, 983, 1093, 1108, 1503
BtsI GCAGTG 1 cut(s) 881
BtsIMutI CAGTG 4 cut(s) 571, 881, 1206, 1480
Cac8I GCNNGC 4 cut(s) 124, 1341, 1442, 1446
CaiI CAGNNNCTG 1 cut(s) 1346
CciI TCATGA 1 cut(s) 925
CfoI GCGC 2 cut(s) 46, 778
Cfr10I RCCGGY 2 cut(s) 112, 284
Cfr13I GGNCC 4 cut(s) 121, 292, 1091, 1467
CseI GACGC 2 cut(s) 83, 118
Csp6I GTAC 4 cut(s) 261, 288, 399, 1211
CspAI ACCGGT 1 cut(s) 284
CviAII CATG 4 cut(s) 389, 554, 719, 926
CviQI GTAC 4 cut(s) 261, 288, 399, 1211
DdeI CTNAG 6 cut(s) 132, 504, 792, 1095, 1248, 1335
DpnI GATC 4 cut(s) 353, 1154, 1269, 1318
DpnII GATC 4 cut(s) 351, 1152, 1267, 1316
DraI TTTAAA 2 cut(s) 1129, 1327
DraIII CACNNNGTG 1 cut(s) 52
Eam1104I CTCTTC 2 cut(s) 445, 566
EarI CTCTTC 2 cut(s) 445, 566
EciI GGCGGA 2 cut(s) 139, 1248
Ecl136II GAGCTC 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 790
Eco24I GRGCYC 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 1091
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 2 cut(s) 426, 959
EcoICRI GAGCTC 1 cut(s) 136
EcoRII CCWGG 3 cut(s) 210, 461, 1463
EcoT38I GRGCYC 1 cut(s) 138
Esp3I CGTCTC 2 cut(s) 101, 174
FaeI CATG 4 cut(s) 392, 557, 722, 929
FaqI GGGAC 2 cut(s) 215, 352
FatI CATG 4 cut(s) 388, 553, 718, 925
FauI CCCGC 2 cut(s) 131, 388
Fnu4HI GCNGC 2 cut(s) 78, 668
FokI GGATG 7 cut(s) 288, 326, 668, 970, 1100, 1115, 1510
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 2 cut(s) 78, 668
FspBI CTAG 4 cut(s) 194, 1029, 1161, 1519
GlaI GCGC 2 cut(s) 45, 777
GluI GCNGC 2 cut(s) 78, 668
GsuI CTGGAG 2 cut(s) 612, 1286
HaeIII GGCC 5 cut(s) 122, 294, 790, 1075, 1468
HapII CCGG 3 cut(s) 100, 113, 285
HgaI GACGC 2 cut(s) 83, 118
HhaI GCGC 2 cut(s) 46, 778
Hin1I GRCGYC 1 cut(s) 110
Hin1II CATG 4 cut(s) 392, 557, 722, 929
Hin6I GCGC 2 cut(s) 44, 776
HinP1I GCGC 2 cut(s) 44, 776
HincII GTYRAC 3 cut(s) 431, 497, 1290
HindII GTYRAC 3 cut(s) 431, 497, 1290
HinfI GANTC 6 cut(s) 146, 299, 1255, 1361, 1504, 1512
HpaI GTTAAC 1 cut(s) 1290
HpaII CCGG 3 cut(s) 100, 113, 285
HphI GGTGA 1 cut(s) 926
Hpy166II GTNNAC 6 cut(s) 39, 55, 223, 431, 497, 1290
Hpy188I TCNGA 5 cut(s) 234, 525, 546, 811, 1297
Hpy188III TCNNGA 5 cut(s) 537, 591, 926, 1007, 1186
Hpy8I GTNNAC 6 cut(s) 39, 55, 223, 431, 497, 1290
HpyAV CCTTC 4 cut(s) 888, 1065, 1351, 1434
HpyCH4III ACNGT 3 cut(s) 566, 976, 1210
HpyCH4V TGCA 6 cut(s) 308, 643, 662, 667, 961, 1046
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 1258
HpyF3I CTNAG 6 cut(s) 132, 504, 792, 1095, 1248, 1335
Hsp92I GRCGYC 1 cut(s) 110
Hsp92II CATG 4 cut(s) 392, 557, 722, 929
HspAI GCGC 2 cut(s) 44, 776
KspAI GTTAAC 1 cut(s) 1290
Kzo9I GATC 4 cut(s) 351, 1152, 1267, 1316
LguI GCTCTTC 1 cut(s) 566
LmnI GCTCC 5 cut(s) 28, 392, 832, 880, 900
Lsp1109I GCAGC 1 cut(s) 679
LweI GCATC 2 cut(s) 649, 1352
MaeI CTAG 4 cut(s) 194, 1029, 1161, 1519
MaeIII GTNAC 4 cut(s) 178, 189, 635, 649
MalI GATC 4 cut(s) 353, 1154, 1269, 1318
MbiI CCGCTC 1 cut(s) 395
MboI GATC 4 cut(s) 351, 1152, 1267, 1316
MboII GAAGA 4 cut(s) 432, 579, 583, 952
MflI RGATCY 3 cut(s) 351, 1152, 1316
MhlI GDGCHC 2 cut(s) 138, 905
MlyI GAGTC 4 cut(s) 155, 1355, 1513, 1521
MmeI TCCRAC 4 cut(s) 253, 789, 999, 1414
MroXI GAANNNNTTC 1 cut(s) 1385
MseI TTAA 5 cut(s) 1128, 1143, 1224, 1289, 1326
MslI CAYNNNNRTG 1 cut(s) 999
MspI CCGG 3 cut(s) 100, 113, 285
MspR9I CCNGG 4 cut(s) 101, 212, 463, 1465
MvaI CCWGG 3 cut(s) 212, 463, 1465
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 2 cut(s) 128, 1258
NciI CCSGG 1 cut(s) 101
NdeII GATC 4 cut(s) 351, 1152, 1267, 1316
NlaIII CATG 4 cut(s) 392, 557, 722, 929
NlaIV GGNNCC 3 cut(s) 535, 876, 1462
NmuCI GTSAC 2 cut(s) 178, 635
NspI RCATGY 2 cut(s) 557, 722
PagI TCATGA 1 cut(s) 925
PceI AGGCCT 1 cut(s) 790
PciI ACATGT 1 cut(s) 553
PciSI GCTCTTC 1 cut(s) 566
PdmI GAANNNNTTC 1 cut(s) 1385
PfeI GAWTC 2 cut(s) 299, 1255
PinAI ACCGGT 1 cut(s) 284
PkrI GCNGC 2 cut(s) 79, 669
PleI GAGTC 4 cut(s) 154, 1355, 1512, 1520
PpsI GAGTC 4 cut(s) 154, 1355, 1512, 1520
PscI ACATGT 1 cut(s) 553
Psp124BI GAGCTC 1 cut(s) 138
Psp6I CCWGG 3 cut(s) 210, 461, 1463
PspGI CCWGG 3 cut(s) 210, 461, 1463
PspN4I GGNNCC 3 cut(s) 535, 876, 1462
PspPI GGNCC 4 cut(s) 121, 292, 1091, 1467
PstNI CAGNNNCTG 1 cut(s) 1346
PsuI RGATCY 3 cut(s) 351, 1152, 1316
RsaI GTAC 4 cut(s) 262, 289, 400, 1212
RsaNI GTAC 4 cut(s) 261, 288, 399, 1211
RseI CAYNNNNRTG 1 cut(s) 999
SacI GAGCTC 1 cut(s) 138
SapI GCTCTTC 1 cut(s) 566
SaqAI TTAA 5 cut(s) 1128, 1143, 1224, 1289, 1326
SatI GCNGC 2 cut(s) 78, 668
Sau3AI GATC 4 cut(s) 351, 1152, 1267, 1316
Sau96I GGNCC 4 cut(s) 121, 292, 1091, 1467
SchI GAGTC 4 cut(s) 155, 1355, 1513, 1521
ScrFI CCNGG 4 cut(s) 101, 212, 463, 1465
SduI GDGCHC 2 cut(s) 138, 905
SfaNI GCATC 2 cut(s) 649, 1352
SfcI CTRYAG 2 cut(s) 174, 486
SgrAI CRCCGGYG 1 cut(s) 112
SinI GGWCC 1 cut(s) 1091
SmiMI CAYNNNNRTG 1 cut(s) 999
SmlI CTYRAG 1 cut(s) 603
SmoI CTYRAG 1 cut(s) 603
SseBI AGGCCT 1 cut(s) 790
SsiI CCGC 7 cut(s) 77, 124, 150, 386, 395, 1259, 1516
SspMI CTAG 4 cut(s) 194, 1029, 1161, 1519
SstI GAGCTC 1 cut(s) 138
StuI AGGCCT 1 cut(s) 790
StyD4I CCNGG 4 cut(s) 99, 210, 461, 1463
TaaI ACNGT 3 cut(s) 566, 976, 1210
TaqI TCGA 4 cut(s) 144, 245, 470, 832
TatI WGTACW 1 cut(s) 260
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 2 cut(s) 299, 1255
Tru1I TTAA 5 cut(s) 1128, 1143, 1224, 1289, 1326
Tru9I TTAA 5 cut(s) 1128, 1143, 1224, 1289, 1326
TscAI CASTG 4 cut(s) 571, 888, 1213, 1480
TseFI GTSAC 2 cut(s) 178, 635
TseI GCWGC 1 cut(s) 667
Tsp45I GTSAC 2 cut(s) 178, 635
TspDTI ATGAA 5 cut(s) 494, 670, 914, 942, 1370
TspGWI ACGGA 1 cut(s) 156
TspRI CASTG 4 cut(s) 571, 888, 1213, 1480
VpaK11BI GGWCC 1 cut(s) 1091
XapI RAATTY 8 cut(s) 361, 697, 836, 864, 920, 929, 1124, 1220
XceI RCATGY 2 cut(s) 557, 722
XcmI CCANNNNNNNNNTGG 2 cut(s) 1213, 1310
XmnI GAANNNNTTC 1 cut(s) 1385
XspI CTAG 4 cut(s) 194, 1029, 1161, 1519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.