Rroxscaffold_3G00256040

Protein TPX2-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005322
Physical Location & Seq
Forward (+)
50484933 .. 50490086
5154 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00256040.1

Sequence Viewer

Length: 1521 bp
ATGGAAATGGAGGAAGATATGGAGGTGGAGCAAGTGTTTACGGCGCACGAAGTGGACATTGACTACGAGTTTGACGCGGCCAGGTTCTTTGATTTTAGTCGGCAGGAGACGCCGGTGGAGGCGCGGCGGGCTGAGCTCTGGTTCGAATCCGCCAAGAGCTATCCTCCTTCTCCTGTTGTGACAAGGATGATACTAGGAGGGGACATCCTCCTTGGAAATGTGAATACCTCCCCAAAATCTAAAGATGTGGAGAATACAAATGGCATTGGCTGTAATATCGGCGCGGGCCAAGAATCGTGTGCAATGGATGTAAATAGCGGAGATTGTGAAGGAATGAATAAAGGGATTTTCGGCAACTTGCAAAGAGTTCTAAATCAATCTCATGGCGCAGGGTTGACATTCAATCATTCAAAAGGTCAAAAGAACTCTTCAGTAAAGCCATCTTTCCCCAGGAAGTCAACTTTGATGAAACCTACAGCAAGTCAACTTGCTAAGCAAAATCGCCCCCTCATATTGGTGGTTCCAGGTGATTCTCAGAATCTGCTGCCTCTTGTTTATGTTTTTCAATGTATTGTGTTTACGCTACATGTTCAAAACAGTGATAAGAGCTTCTGTAGCTCTTCTGGAGTTGAAACTCAAGCTACCAAAAGGCAGAAGTTAGAGGGAGGTCACTCGCAAAAGGTTACTACCGAAGCAATGCAGCAAACTAATTTCATCCACAAGGCACCTAAAAAGGTCGAAACTTTTGATAAGACGCATGCCAAGTTGAAAATTACTATTCCAATAGAGCCTGACTTTGAAACGACATATAGGGCGCAAAGAATAAGGCCTAAGAATGGATCAGTGTTGGAACAAGTGCCATCAGCTCCTCGAAAATTCAAAGCCCGTCCACTGAACAAAAAAATTTTTGAGGCTCCCTCACTGCCACTTCCAAAGAGGAGCACTCCAAAATTGCCAGAATTTCATGAATTTCACCTGAAGACAATGGAAAGAGCTATGCAAAATACATCTACTGTTCCATTATCCTCACATCATTTCAATGGTCAAGAGAAGGTGTTGGACAAACCTAGTGCTTCCACTGTTGCAGGAAATGGAAACAGAGAACCCAGAAGGCCGAGTAAATTGGATGGTCTTAGGCAGGATGGAAGTGATGTAATGCCAAAATTTAAAGCTCGTCCTCTTAATAAAAAGATCTTTTCTAGCAAAGGGGATATCGGTGTTTTCTGGAATAGGAAAAGAGAAACCACTGTACCAATGGAATTTAATTTTCAGACAGAGAGGAGAACTCAGCAGATTCCGCCAACTGATCTTTTCAGTAAGTGCCAGCTCTCTTTAACATCTGAACTCCAGCCAAATAATGGAACTCAGTTGAACTTGCCTCAGCCAACATCTGTATCAGTGAAGATAATGGTAAAAGAGAAATCTTCACTGTTTGGTGGAAAGCAAGCTCTATTTGGGGGTGCCAGGTCCATCAGTGATGTTGATAACAAATCAAGGATGAGGAGTTTGGGAGTCCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2_importin

Protein Analysis

506

Amino Acids

56.48

Weight (kDa)

9.59

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2_importin PF12214 256 - 423 4e-45 Cell cycle regulated microtubule associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 724, 1460
AccB7I CCANNNNNTGG 1 cut(s) 1358
AccII CGCG 3 cut(s) 77, 124, 284
AciI CCGC 8 cut(s) 77, 124, 127, 150, 284, 318, 1298, 1516
AclWI GGATC 1 cut(s) 847
AcoI YGGCCR 1 cut(s) 78
AcsI RAATTY 6 cut(s) 875, 903, 959, 968, 1163, 1259
AcuI CTGAAG 2 cut(s) 414, 998
AcyI GRCGYC 1 cut(s) 110
AdeI CACNNNGTG 1 cut(s) 52
AfaI GTAC 1 cut(s) 1251
AfiI CCNNNNNNNGG 3 cut(s) 514, 836, 1358
AflIII ACRYGT 1 cut(s) 586
AjnI CCWGG 4 cut(s) 80, 449, 523, 1463
Alw21I GWGCWC 2 cut(s) 138, 944
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 1 cut(s) 847
AlwNI CAGNNNCTG 1 cut(s) 541
AoxI GGCC 4 cut(s) 78, 286, 827, 1112
ApeKI GCWGC 2 cut(s) 544, 700
ApoI RAATTY 6 cut(s) 875, 903, 959, 968, 1163, 1259
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
AspLEI GCGC 5 cut(s) 46, 124, 284, 389, 817
AspS9I GGNCC 2 cut(s) 286, 1467
AsuHPI GGTGA 2 cut(s) 539, 965
AsuII TTCGAA 1 cut(s) 144
AvaII GGWCC 1 cut(s) 1467
BaeI ACNNNNGTAYC 4 cut(s) 1233, 1266, 1377, 1410
BanI GGYRCC 2 cut(s) 724, 1460
BanII GRGCYC 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 986
Bbv12I GWGCWC 2 cut(s) 138, 944
BbvCI CCTCAGC 1 cut(s) 1380
BbvI GCAGC 2 cut(s) 531, 712
BccI CCATC 5 cut(s) 448, 868, 1121, 1136, 1478
BceAI ACGGC 1 cut(s) 57
BciT130I CCWGG 4 cut(s) 82, 451, 525, 1465
BcoDI GTCTC 1 cut(s) 101
BfaI CTAG 4 cut(s) 194, 1068, 1200, 1519
BfmI CTRYAG 2 cut(s) 474, 613
BglII AGATCT 1 cut(s) 1191
BisI GCNGC 4 cut(s) 78, 125, 545, 701
BlpI GCTNAGC 2 cut(s) 132, 492
BlsI GCNGC 4 cut(s) 79, 126, 546, 702
Bme1390I CCNGG 4 cut(s) 82, 451, 525, 1465
Bme18I GGWCC 1 cut(s) 1467
BmgT120I GGNCC 2 cut(s) 286, 1467
BmiI GGNNCC 4 cut(s) 522, 726, 915, 1462
BmrFI CCNGG 4 cut(s) 82, 451, 525, 1465
BpiI GAAGAC 1 cut(s) 986
BplI GAGNNNNNCTC 8 cut(s) 148, 180, 902, 934, 928, 960, 1270, 1302
BpmI CTGGAG 2 cut(s) 645, 1331
Bpu10I CCTNAGC 1 cut(s) 1380
Bpu1102I GCTNAGC 2 cut(s) 132, 492
Bpu14I TTCGAA 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 621
BsaHI GRCGYC 1 cut(s) 110
BsaJI CCNNGG 2 cut(s) 211, 449
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 3 cut(s) 514, 836, 1358
Bse118I RCCGGY 1 cut(s) 112
Bse3DI GCAATG 2 cut(s) 309, 702
BseBI CCWGG 4 cut(s) 82, 451, 525, 1465
BseDI CCNNGG 2 cut(s) 211, 449
BseGI GGATG 7 cut(s) 192, 204, 313, 714, 1132, 1147, 1503
BseLI CCNNNNNNNGG 3 cut(s) 514, 836, 1358
BseMI GCAATG 2 cut(s) 309, 702
BseMII CTCAG 5 cut(s) 123, 548, 1301, 1379, 1394
BseRI GAGGAG 4 cut(s) 858, 952, 1294, 1516
BseXI GCAGC 2 cut(s) 531, 712
Bsh1236I CGCG 3 cut(s) 77, 124, 284
BshFI GGCC 4 cut(s) 80, 288, 829, 1114
BshNI GGYRCC 2 cut(s) 724, 1460
BsiHKAI GWGCWC 2 cut(s) 138, 944
BsiSI CCGG 1 cut(s) 113
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 3 cut(s) 514, 836, 1358
BsmAI GTCTC 1 cut(s) 101
BsmBI CGTCTC 1 cut(s) 101
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 4 cut(s) 80, 288, 829, 1114
Bsp119I TTCGAA 1 cut(s) 144
Bsp1286I GDGCHC 2 cut(s) 138, 944
Bsp143I GATC 3 cut(s) 839, 1191, 1306
Bsp1720I GCTNAGC 2 cut(s) 132, 492
BspACI CCGC 8 cut(s) 77, 124, 127, 150, 284, 318, 1298, 1516
BspANI GGCC 4 cut(s) 80, 288, 829, 1114
BspCNI CTCAG 5 cut(s) 124, 547, 1300, 1378, 1393
BspFNI CGCG 3 cut(s) 77, 124, 284
BspHI TCATGA 1 cut(s) 964
BspLI GGNNCC 4 cut(s) 522, 726, 915, 1462
BspPI GGATC 1 cut(s) 847
BspQI GCTCTTC 1 cut(s) 625
BspT104I TTCGAA 1 cut(s) 144
BspT107I GGYRCC 2 cut(s) 724, 1460
BsrDI GCAATG 2 cut(s) 309, 702
BsrFI RCCGGY 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 112
BssECI CCNNGG 2 cut(s) 211, 449
BssMI GATC 3 cut(s) 839, 1191, 1306
BssNI GRCGYC 1 cut(s) 110
BssT1I CCWWGG 1 cut(s) 211
Bst2UI CCWGG 4 cut(s) 82, 451, 525, 1465
Bst4CI ACNGT 5 cut(s) 599, 1015, 1081, 1249, 1431
Bst6I CTCTTC 2 cut(s) 433, 625
BstACI GRCGYC 1 cut(s) 110
BstBI TTCGAA 1 cut(s) 144
BstC8I GCNNGC 5 cut(s) 129, 286, 759, 1325, 1446
BstDEI CTNAG 8 cut(s) 132, 492, 534, 831, 1133, 1287, 1365, 1380
BstF5I GGATG 7 cut(s) 192, 204, 313, 714, 1132, 1147, 1503
BstFNI CGCG 3 cut(s) 77, 124, 284
BstHHI GCGC 5 cut(s) 46, 124, 284, 389, 817
BstKTI GATC 3 cut(s) 842, 1194, 1309
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 3 cut(s) 839, 1191, 1306
BstMWI GCNNNNNNNGC 5 cut(s) 109, 128, 133, 615, 1297
BstNI CCWGG 4 cut(s) 82, 451, 525, 1465
BstNSI RCATGY 2 cut(s) 590, 761
BstSCI CCNGG 4 cut(s) 80, 449, 523, 1463
BstSFI CTRYAG 2 cut(s) 474, 613
BstUI CGCG 3 cut(s) 77, 124, 284
BstV1I GCAGC 2 cut(s) 531, 712
BstV2I GAAGAC 1 cut(s) 986
BstX2I RGATCY 1 cut(s) 1191
BstYI RGATCY 1 cut(s) 1191
BsuRI GGCC 4 cut(s) 80, 288, 829, 1114
BtsCI GGATG 7 cut(s) 192, 204, 313, 714, 1132, 1147, 1503
BtsI GCAGTG 1 cut(s) 920
BtsIMutI CAGTG 9 cut(s) 604, 849, 890, 920, 1077, 1245, 1404, 1427, 1480
Cac8I GCNNGC 5 cut(s) 129, 286, 759, 1325, 1446
CaiI CAGNNNCTG 1 cut(s) 541
CciI TCATGA 1 cut(s) 964
CfoI GCGC 5 cut(s) 46, 124, 284, 389, 817
Cfr10I RCCGGY 1 cut(s) 112
Cfr13I GGNCC 2 cut(s) 286, 1467
CseI GACGC 3 cut(s) 83, 118, 763
Csp6I GTAC 1 cut(s) 1250
CviAII CATG 4 cut(s) 383, 587, 758, 965
CviQI GTAC 1 cut(s) 1250
DdeI CTNAG 8 cut(s) 132, 492, 534, 831, 1133, 1287, 1365, 1380
DpnI GATC 3 cut(s) 841, 1193, 1308
DpnII GATC 3 cut(s) 839, 1191, 1306
DraI TTTAAA 1 cut(s) 1168
DraIII CACNNNGTG 1 cut(s) 52
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 2 cut(s) 433, 625
EarI CTCTTC 2 cut(s) 433, 625
EciI GGCGGA 2 cut(s) 139, 1287
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 211
Eco147I AGGCCT 1 cut(s) 829
Eco24I GRGCYC 1 cut(s) 138
Eco32I GATATC 1 cut(s) 1213
Eco47I GGWCC 1 cut(s) 1467
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 2 cut(s) 414, 998
EcoICRI GAGCTC 1 cut(s) 136
EcoRII CCWGG 4 cut(s) 80, 449, 523, 1463
EcoRV GATATC 1 cut(s) 1213
EcoT14I CCWWGG 1 cut(s) 211
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 211
Esp3I CGTCTC 1 cut(s) 101
FaeI CATG 4 cut(s) 386, 590, 761, 968
FaqI GGGAC 1 cut(s) 215
FatI CATG 4 cut(s) 382, 586, 757, 964
FauI CCCGC 2 cut(s) 120, 277
Fnu4HI GCNGC 4 cut(s) 78, 125, 545, 701
FokI GGATG 7 cut(s) 191, 199, 320, 701, 1139, 1154, 1510
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 4 cut(s) 78, 125, 545, 701
FspBI CTAG 4 cut(s) 194, 1068, 1200, 1519
GlaI GCGC 5 cut(s) 45, 123, 283, 388, 816
GluI GCNGC 4 cut(s) 78, 125, 545, 701
GsuI CTGGAG 2 cut(s) 645, 1331
HaeIII GGCC 4 cut(s) 80, 288, 829, 1114
HapII CCGG 1 cut(s) 113
HgaI GACGC 3 cut(s) 83, 118, 763
HhaI GCGC 5 cut(s) 46, 124, 284, 389, 817
Hin1I GRCGYC 1 cut(s) 110
Hin1II CATG 4 cut(s) 386, 590, 761, 968
Hin6I GCGC 5 cut(s) 44, 122, 282, 387, 815
HinP1I GCGC 5 cut(s) 44, 122, 282, 387, 815
HincII GTYRAC 3 cut(s) 396, 459, 485
HindII GTYRAC 3 cut(s) 396, 459, 485
HinfI GANTC 6 cut(s) 146, 293, 530, 538, 1294, 1512
HpaII CCGG 1 cut(s) 113
HphI GGTGA 2 cut(s) 539, 965
Hpy166II GTNNAC 7 cut(s) 39, 55, 396, 459, 485, 579, 890
Hpy188I TCNGA 3 cut(s) 537, 1272, 1342
Hpy188III TCNNGA 4 cut(s) 624, 965, 1046, 1225
Hpy8I GTNNAC 7 cut(s) 39, 55, 396, 459, 485, 579, 890
HpyAV CCTTC 4 cut(s) 177, 323, 1045, 1104
HpyCH4III ACNGT 5 cut(s) 599, 1015, 1081, 1249, 1431
HpyCH4V TGCA 5 cut(s) 302, 361, 700, 1000, 1085
HpyF10VI GCNNNNNNNGC 5 cut(s) 109, 128, 133, 615, 1297
HpyF3I CTNAG 8 cut(s) 132, 492, 534, 831, 1133, 1287, 1365, 1380
Hsp92I GRCGYC 1 cut(s) 110
Hsp92II CATG 4 cut(s) 386, 590, 761, 968
HspAI GCGC 5 cut(s) 44, 122, 282, 387, 815
Kzo9I GATC 3 cut(s) 839, 1191, 1306
LguI GCTCTTC 1 cut(s) 625
LmnI GCTCC 4 cut(s) 28, 871, 919, 939
Lsp1109I GCAGC 2 cut(s) 531, 712
MaeI CTAG 4 cut(s) 194, 1068, 1200, 1519
MaeIII GTNAC 3 cut(s) 178, 668, 682
MalI GATC 3 cut(s) 841, 1193, 1308
MboI GATC 3 cut(s) 839, 1191, 1306
MboII GAAGA 6 cut(s) 26, 420, 612, 991, 1414, 1416
MflI RGATCY 1 cut(s) 1191
MhlI GDGCHC 2 cut(s) 138, 944
MlyI GAGTC 1 cut(s) 1521
MmeI TCCRAC 2 cut(s) 828, 1038
MseI TTAA 4 cut(s) 1167, 1182, 1263, 1334
MslI CAYNNNNRTG 2 cut(s) 515, 1038
MspI CCGG 1 cut(s) 113
MspR9I CCNGG 4 cut(s) 82, 451, 525, 1465
MvaI CCWGG 4 cut(s) 82, 451, 525, 1465
MvnI CGCG 3 cut(s) 77, 124, 284
MwoI GCNNNNNNNGC 5 cut(s) 109, 128, 133, 615, 1297
NdeII GATC 3 cut(s) 839, 1191, 1306
NlaIII CATG 4 cut(s) 386, 590, 761, 968
NlaIV GGNNCC 4 cut(s) 522, 726, 915, 1462
NmeAIII GCCGAG 1 cut(s) 1140
NmuCI GTSAC 2 cut(s) 178, 668
NspI RCATGY 2 cut(s) 590, 761
NspV TTCGAA 1 cut(s) 144
PaeI GCATGC 1 cut(s) 761
PagI TCATGA 1 cut(s) 964
PceI AGGCCT 1 cut(s) 829
PciI ACATGT 1 cut(s) 586
PciSI GCTCTTC 1 cut(s) 625
PfeI GAWTC 5 cut(s) 146, 293, 530, 538, 1294
PflMI CCANNNNNTGG 1 cut(s) 1358
PkrI GCNGC 4 cut(s) 79, 126, 546, 702
PleI GAGTC 1 cut(s) 1520
PpsI GAGTC 1 cut(s) 1520
PscI ACATGT 1 cut(s) 586
Psp124BI GAGCTC 1 cut(s) 138
Psp6I CCWGG 4 cut(s) 80, 449, 523, 1463
PspGI CCWGG 4 cut(s) 80, 449, 523, 1463
PspN4I GGNNCC 4 cut(s) 522, 726, 915, 1462
PspPI GGNCC 2 cut(s) 286, 1467
PstNI CAGNNNCTG 1 cut(s) 541
PsuI RGATCY 1 cut(s) 1191
RsaI GTAC 1 cut(s) 1251
RsaNI GTAC 1 cut(s) 1250
RseI CAYNNNNRTG 2 cut(s) 515, 1038
SacI GAGCTC 1 cut(s) 138
SapI GCTCTTC 1 cut(s) 625
SaqAI TTAA 4 cut(s) 1167, 1182, 1263, 1334
SatI GCNGC 4 cut(s) 78, 125, 545, 701
Sau3AI GATC 3 cut(s) 839, 1191, 1306
Sau96I GGNCC 2 cut(s) 286, 1467
SchI GAGTC 1 cut(s) 1521
ScrFI CCNGG 4 cut(s) 82, 451, 525, 1465
SduI GDGCHC 2 cut(s) 138, 944
SfcI CTRYAG 2 cut(s) 474, 613
SfuI TTCGAA 1 cut(s) 144
SgrAI CRCCGGYG 1 cut(s) 112
SinI GGWCC 1 cut(s) 1467
SmiMI CAYNNNNRTG 2 cut(s) 515, 1038
SmlI CTYRAG 1 cut(s) 636
SmoI CTYRAG 1 cut(s) 636
SphI GCATGC 1 cut(s) 761
SseBI AGGCCT 1 cut(s) 829
SsiI CCGC 8 cut(s) 77, 124, 127, 150, 284, 318, 1298, 1516
SspMI CTAG 4 cut(s) 194, 1068, 1200, 1519
SstI GAGCTC 1 cut(s) 138
StuI AGGCCT 1 cut(s) 829
StyD4I CCNGG 4 cut(s) 80, 449, 523, 1463
StyI CCWWGG 1 cut(s) 211
TaaI ACNGT 5 cut(s) 599, 1015, 1081, 1249, 1431
TaqI TCGA 3 cut(s) 144, 738, 871
TauI GCSGC 2 cut(s) 80, 127
TfiI GAWTC 5 cut(s) 146, 293, 530, 538, 1294
Tru1I TTAA 4 cut(s) 1167, 1182, 1263, 1334
Tru9I TTAA 4 cut(s) 1167, 1182, 1263, 1334
TscAI CASTG 9 cut(s) 604, 849, 897, 927, 1084, 1252, 1404, 1434, 1480
TseFI GTSAC 2 cut(s) 178, 668
TseI GCWGC 2 cut(s) 544, 700
Tsp45I GTSAC 2 cut(s) 178, 668
TspDTI ATGAA 5 cut(s) 350, 482, 703, 953, 981
TspRI CASTG 9 cut(s) 604, 849, 897, 927, 1084, 1252, 1404, 1434, 1480
Van91I CCANNNNNTGG 1 cut(s) 1358
VpaK11BI GGWCC 1 cut(s) 1467
XapI RAATTY 6 cut(s) 875, 903, 959, 968, 1163, 1259
XceI RCATGY 2 cut(s) 590, 761
XcmI CCANNNNNNNNNTGG 2 cut(s) 1252, 1355
XspI CTAG 4 cut(s) 194, 1068, 1200, 1519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.