MD04G1076100.v1.1

Protein TPX2-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005322
Physical Location & Seq
Reverse (-)
10247612 .. 10252890
5279 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1076100.v1.1.491

Sequence Viewer

Length: 1521 bp
ATGGAAATGGAGGACGATATGGAGGTTGAGGAAGTGTTTATGGCGCAGGAAGTGGACGTTGACTACGAGTTCGATGCAGCTCGGTACTTTGACTTCACTAGGGAGGAGACTCCGGCTGAGGCTCGCCGCGCCGAGCTCTGGTTCGAGTCCGCCAAGAGCTACCCGCCGTCTCCTGTTGTGACAAGGTTAATGCTTGGACCGGAAATCCTCCTGGAAAACGTAAACACCTCCCCAAAATGTGATGTGGAGAATGCAAATGATGGAGATTGTGATATCGGTGTGGGCACAGAATCATGTGTAATGGATGTAAACAGCCGGGATGAAGGAATGAATAGAGGGACCTTTGGAAATTTACAAAAAGTTCTAAATCAACCGAATGGAGCTGGTACAGGACTGACATTCAACAGTCACCTAACCGGTGATAAGCTTAAAGGTCAGAGTAATGCTTCTGTGAAGCCATCTTTCCCCAGGAGCTCAACTTTGATGAAGCCCACAGCAAGTCAGCTCGCTAAGCAAAATCTGAAGCCTCAAAATGGTGGTTCCAGATTCCAAATGCTGCATGTTCAAAACAATGAGAAGAGCTTGTGTAGTTCTTCTGGGGTTGAAAGTCAAGCTCCCAAGAGACAGAAGCTAGATGGAGGACACTTGCGTAAGGTTACTGATGCAAAGCAGCAAACTAATTTTGTCCACAAGGTACCTAAAAAGGATGAAACTTTCGACAAAACTACTGCACACCCTAAGTTGAGACTTACCATTCCAAGAGAGCCTGACTTTGAAACGGCACATAGGGCACACAGGATTAGGCCTAACAATGGATCTATGTTAGAACAAGTGACATCAACTCATCGAAAATTCAAAGCTCGTCCATTGAACCGAAAAATTTTCGAGGCTCCTTCATTGCCACTTCCAACAAGGAGCACTCCAAAGTTGCCGGAATTTCAAGAATTTCACCTGAAGACAATGGAAAGGGCTATGCAAAATGCATCGGCTGTTTCATCATCCTCACATTATCTCAATGATCATGATAAGGGTTTGGAAAAACCTAGCATTTCTACCGTTGCGGAAAACAGAAAAGGAGAATCCAGAAGACCAAATACAGTGGAATGTCCTAAGCAGGATGGAAATGCAGGAATGCAAACATTTAAAGCTCGTCCTCTTAACAAAAAGATATTTTCTAGCAAAGGGAATATTGGTGTTTTCTGGAATAGCAAGCGAGAAACCACTGTACCAATGGAATTTAATTTTCAGACAGACAGAAGAGTACAGCAATTTCCGCCAACTGAACTTTTTAGTAAGCTCTCTTTAACCACTGAGCTCCAGCCGAATAATGGATCACGGGTGAAACTATCCCAGCCTACATCTTTGTTTAAGAAGGACTCAAAAGAAAATAGATCAACTGTTTTCCAACAAGAACATAAGATAAAAGAAAAACCTTCTCTGTTTGGTGGAAAGCAGGCTCAAGACGGGTGCACGACCGATGTTGATAAGAAATTGAGGATGAGTAGGTTGGGCGTTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2_importin

Protein Analysis

507

Amino Acids

56.96

Weight (kDa)

9.3

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2_importin PF12214 248 - 415 1e-46 Cell cycle regulated microtubule associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 694
AccB1I GGYRCC 1 cut(s) 694
AccII CGCG 1 cut(s) 129
AciI CCGC 5 cut(s) 127, 150, 164, 1061, 1274
AclWI GGATC 2 cut(s) 823, 1339
AcsI RAATTY 6 cut(s) 349, 851, 879, 935, 944, 1235
AcuI CTGAAG 2 cut(s) 542, 974
AfaI GTAC 5 cut(s) 86, 388, 696, 1227, 1263
AfiI CCNNNNNNNGG 4 cut(s) 138, 533, 812, 1328
AgeI ACCGGT 1 cut(s) 416
AgsI TTSAA 7 cut(s) 403, 566, 605, 776, 856, 871, 941
AjnI CCWGG 2 cut(s) 210, 467
Alw21I GWGCWC 5 cut(s) 138, 476, 920, 1317, 1472
Alw26I GTCTC 4 cut(s) 101, 174, 616, 739
Alw44I GTGCAC 1 cut(s) 1468
AlwI GGATC 2 cut(s) 823, 1339
AoxI GGCC 1 cut(s) 803
ApaLI GTGCAC 1 cut(s) 1468
ApeKI GCWGC 3 cut(s) 77, 556, 670
ApoI RAATTY 6 cut(s) 349, 851, 879, 935, 944, 1235
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
AsiGI ACCGGT 1 cut(s) 416
Asp718I GGTACC 1 cut(s) 694
AspLEI GCGC 2 cut(s) 46, 131
AspS9I GGNCC 2 cut(s) 197, 339
AsuC2I CCSGG 1 cut(s) 317
AsuHPI GGTGA 4 cut(s) 401, 431, 941, 1351
AvaII GGWCC 2 cut(s) 197, 339
BaeGI GKGCMC 3 cut(s) 287, 793, 1472
BaeI ACNNNNGTAYC 2 cut(s) 1209, 1242
BanI GGYRCC 1 cut(s) 694
BanII GRGCYC 3 cut(s) 138, 476, 1317
BarI GAAGNNNNNNTAC 4 cut(s) 77, 109, 1078, 1110
BbsI GAAGAC 2 cut(s) 962, 1093
Bbv12I GWGCWC 5 cut(s) 138, 476, 920, 1317, 1472
BbvCI CCTCAGC 1 cut(s) 117
BbvI GCAGC 3 cut(s) 89, 543, 682
BccI CCATC 4 cut(s) 254, 466, 629, 1112
BceAI ACGGC 2 cut(s) 151, 795
BcgI CGANNNNNNTGC 2 cut(s) 56, 90
BciT130I CCWGG 2 cut(s) 212, 469
BclI TGATCA 1 cut(s) 1018
BcnI CCSGG 1 cut(s) 317
BcoDI GTCTC 4 cut(s) 101, 174, 616, 739
BfaI CTAG 4 cut(s) 99, 632, 1044, 1176
BisI GCNGC 4 cut(s) 78, 127, 557, 671
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 4 cut(s) 79, 128, 558, 672
Bme1390I CCNGG 3 cut(s) 212, 317, 469
Bme18I GGWCC 2 cut(s) 197, 339
BmgT120I GGNCC 2 cut(s) 197, 339
BmiI GGNNCC 4 cut(s) 340, 541, 696, 891
BmrFI CCNGG 3 cut(s) 212, 317, 469
BmsI GCATC 3 cut(s) 64, 652, 992
BpiI GAAGAC 2 cut(s) 962, 1093
BpmI CTGGAG 1 cut(s) 1301
Bpu10I CCTNAGC 2 cut(s) 117, 1110
Bpu1102I GCTNAGC 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 1443
BpuMI CCSGG 1 cut(s) 317
BsaJI CCNNGG 1 cut(s) 467
BsaWI WCCGGW 2 cut(s) 199, 416
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 533, 812, 1328
Bse118I RCCGGY 1 cut(s) 416
Bse3DI GCAATG 1 cut(s) 896
BseBI CCWGG 2 cut(s) 212, 469
BseDI CCNNGG 1 cut(s) 467
BseGI GGATG 6 cut(s) 310, 325, 712, 998, 1123, 1503
BseLI CCNNNNNNNGG 4 cut(s) 138, 533, 812, 1328
BseMI GCAATG 1 cut(s) 896
BseMII CTCAG 2 cut(s) 108, 1302
BseRI GAGGAG 1 cut(s) 119
BseSI GKGCMC 3 cut(s) 287, 793, 1472
BseXI GCAGC 3 cut(s) 89, 543, 682
BseYI CCCAGC 1 cut(s) 1350
BsgI GTGCAG 1 cut(s) 714
Bsh1236I CGCG 1 cut(s) 129
Bsh1285I CGRYCG 1 cut(s) 1476
BshFI GGCC 1 cut(s) 805
BshNI GGYRCC 1 cut(s) 694
BshTI ACCGGT 1 cut(s) 416
BsiEI CGRYCG 1 cut(s) 1476
BsiHKAI GWGCWC 5 cut(s) 138, 476, 920, 1317, 1472
BsiSI CCGG 5 cut(s) 113, 200, 316, 417, 932
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 4 cut(s) 138, 533, 812, 1328
BsmAI GTCTC 4 cut(s) 101, 174, 616, 739
BsmBI CGTCTC 1 cut(s) 174
BsmFI GGGAC 1 cut(s) 352
BsmI GAATGC 2 cut(s) 256, 1137
BsnI GGCC 1 cut(s) 805
Bsp1286I GDGCHC 7 cut(s) 138, 287, 476, 793, 920, 1317, 1472
Bsp143I GATC 4 cut(s) 815, 1018, 1331, 1391
Bsp1720I GCTNAGC 1 cut(s) 510
BspACI CCGC 5 cut(s) 127, 150, 164, 1061, 1274
BspANI GGCC 1 cut(s) 805
BspCNI CTCAG 2 cut(s) 109, 1303
BspFNI CGCG 1 cut(s) 129
BspHI TCATGA 1 cut(s) 1021
BspLI GGNNCC 4 cut(s) 340, 541, 696, 891
BspPI GGATC 2 cut(s) 823, 1339
BspQI GCTCTTC 1 cut(s) 572
BspT107I GGYRCC 1 cut(s) 694
BsrDI GCAATG 1 cut(s) 896
BsrFI RCCGGY 1 cut(s) 416
BssAI RCCGGY 1 cut(s) 416
BssECI CCNNGG 1 cut(s) 467
BssMI GATC 4 cut(s) 815, 1018, 1331, 1391
Bst2UI CCWGG 2 cut(s) 212, 469
Bst4CI ACNGT 5 cut(s) 407, 1057, 1099, 1225, 1399
Bst6I CTCTTC 2 cut(s) 572, 1252
BstC8I GCNNGC 4 cut(s) 124, 507, 1211, 1455
BstDEI CTNAG 5 cut(s) 117, 510, 738, 1110, 1311
BstF5I GGATG 6 cut(s) 310, 325, 712, 998, 1123, 1503
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 2 cut(s) 46, 131
BstKTI GATC 4 cut(s) 818, 1021, 1334, 1394
BstMAI GTCTC 4 cut(s) 101, 174, 616, 739
BstMBI GATC 4 cut(s) 815, 1018, 1331, 1391
BstMCI CGRYCG 1 cut(s) 1476
BstMWI GCNNNNNNNGC 4 cut(s) 128, 511, 788, 1273
BstNI CCWGG 2 cut(s) 212, 469
BstNSI RCATGY 1 cut(s) 563
BstSCI CCNGG 3 cut(s) 210, 315, 467
BstSLI GKGCMC 3 cut(s) 287, 793, 1472
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 3 cut(s) 89, 543, 682
BstV2I GAAGAC 2 cut(s) 962, 1093
BstX2I RGATCY 1 cut(s) 815
BstYI RGATCY 1 cut(s) 815
BsuRI GGCC 1 cut(s) 805
BtsCI GGATG 6 cut(s) 310, 325, 712, 998, 1123, 1503
BtsIMutI CAGTG 3 cut(s) 1104, 1221, 1308
Cac8I GCNNGC 4 cut(s) 124, 507, 1211, 1455
CciI TCATGA 1 cut(s) 1021
CfoI GCGC 2 cut(s) 46, 131
Cfr10I RCCGGY 1 cut(s) 416
Cfr13I GGNCC 2 cut(s) 197, 339
Csp6I GTAC 5 cut(s) 85, 387, 695, 1226, 1262
CspAI ACCGGT 1 cut(s) 416
CspCI CAANNNNNGTGG 2 cut(s) 1080, 1115
CviAII CATG 3 cut(s) 294, 560, 1022
CviQI GTAC 5 cut(s) 85, 387, 695, 1226, 1262
DdeI CTNAG 5 cut(s) 117, 510, 738, 1110, 1311
DpnI GATC 4 cut(s) 817, 1020, 1333, 1393
DpnII GATC 4 cut(s) 815, 1018, 1331, 1391
DraI TTTAAA 1 cut(s) 1144
Eam1104I CTCTTC 2 cut(s) 572, 1252
EarI CTCTTC 2 cut(s) 572, 1252
EciI GGCGGA 2 cut(s) 139, 1263
Ecl136II GAGCTC 3 cut(s) 136, 474, 1315
Eco147I AGGCCT 1 cut(s) 805
Eco24I GRGCYC 3 cut(s) 138, 476, 1317
Eco32I GATATC 1 cut(s) 274
Eco47I GGWCC 2 cut(s) 197, 339
Eco53kI GAGCTC 3 cut(s) 136, 474, 1315
Eco57I CTGAAG 2 cut(s) 542, 974
EcoICRI GAGCTC 3 cut(s) 136, 474, 1315
EcoO109I RGGNCCY 1 cut(s) 339
EcoRII CCWGG 2 cut(s) 210, 467
EcoRV GATATC 1 cut(s) 274
EcoT22I ATGCAT 1 cut(s) 985
EcoT38I GRGCYC 3 cut(s) 138, 476, 1317
Esp3I CGTCTC 1 cut(s) 174
FaeI CATG 3 cut(s) 297, 563, 1025
FaiI YATR 9 cut(s) 20, 41, 295, 561, 786, 821, 974, 1023, 1416
FaqI GGGAC 1 cut(s) 352
FatI CATG 3 cut(s) 293, 559, 1021
FauI CCCGC 1 cut(s) 171
FbaI TGATCA 1 cut(s) 1018
Fnu4HI GCNGC 4 cut(s) 78, 127, 557, 671
FokI GGATG 6 cut(s) 317, 332, 719, 985, 1130, 1510
FriOI GRGCYC 3 cut(s) 138, 476, 1317
Fsp4HI GCNGC 4 cut(s) 78, 127, 557, 671
FspBI CTAG 4 cut(s) 99, 632, 1044, 1176
GlaI GCGC 2 cut(s) 45, 130
GluI GCNGC 4 cut(s) 78, 127, 557, 671
GsaI CCCAGC 1 cut(s) 1354
GsuI CTGGAG 1 cut(s) 1301
HaeIII GGCC 1 cut(s) 805
HapII CCGG 5 cut(s) 113, 200, 316, 417, 932
HhaI GCGC 2 cut(s) 46, 131
Hin1II CATG 3 cut(s) 297, 563, 1025
Hin6I GCGC 2 cut(s) 44, 129
HinP1I GCGC 2 cut(s) 44, 129
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 6 cut(s) 109, 146, 290, 546, 1079, 1376
HpaII CCGG 5 cut(s) 113, 200, 316, 417, 932
HphI GGTGA 4 cut(s) 401, 431, 941, 1351
Hpy166II GTNNAC 6 cut(s) 55, 61, 223, 310, 688, 1470
Hpy188I TCNGA 3 cut(s) 438, 522, 1248
Hpy188III TCNNGA 6 cut(s) 543, 941, 1022, 1083, 1201, 1460
Hpy8I GTNNAC 6 cut(s) 55, 61, 223, 310, 688, 1470
HpyAV CCTTC 4 cut(s) 317, 903, 1366, 1443
HpyCH4III ACNGT 5 cut(s) 407, 1057, 1099, 1225, 1399
HpyCH4IV ACGT 2 cut(s) 57, 219
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 511, 788, 1273
HpyF3I CTNAG 5 cut(s) 117, 510, 738, 1110, 1311
HpySE526I ACGT 2 cut(s) 57, 219
Hsp92II CATG 3 cut(s) 297, 563, 1025
HspAI GCGC 2 cut(s) 44, 129
KpnI GGTACC 1 cut(s) 698
Ksp22I TGATCA 1 cut(s) 1018
Kzo9I GATC 4 cut(s) 815, 1018, 1331, 1391
LguI GCTCTTC 1 cut(s) 572
LmnI GCTCC 6 cut(s) 380, 471, 619, 895, 915, 1320
Lsp1109I GCAGC 3 cut(s) 89, 543, 682
LweI GCATC 3 cut(s) 64, 652, 992
MaeI CTAG 4 cut(s) 99, 632, 1044, 1176
MaeII ACGT 2 cut(s) 57, 219
MaeIII GTNAC 4 cut(s) 178, 407, 655, 832
MalI GATC 4 cut(s) 817, 1020, 1333, 1393
MboI GATC 4 cut(s) 815, 1018, 1331, 1391
MboII GAAGA 5 cut(s) 585, 589, 967, 1098, 1269
MflI RGATCY 1 cut(s) 815
MhlI GDGCHC 7 cut(s) 138, 287, 476, 793, 920, 1317, 1472
MlyI GAGTC 3 cut(s) 103, 155, 1370
MmeI TCCRAC 2 cut(s) 932, 1429
Mph1103I ATGCAT 1 cut(s) 985
MseI TTAA 7 cut(s) 188, 429, 1143, 1158, 1239, 1304, 1368
MspI CCGG 5 cut(s) 113, 200, 316, 417, 932
MspR9I CCNGG 3 cut(s) 212, 317, 469
Mva1269I GAATGC 2 cut(s) 256, 1137
MvaI CCWGG 2 cut(s) 212, 469
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 4 cut(s) 128, 511, 788, 1273
NciI CCSGG 1 cut(s) 317
NdeII GATC 4 cut(s) 815, 1018, 1331, 1391
NlaIII CATG 3 cut(s) 297, 563, 1025
NlaIV GGNNCC 4 cut(s) 340, 541, 696, 891
NmeAIII GCCGAG 1 cut(s) 157
NmuCI GTSAC 3 cut(s) 178, 407, 832
NsiI ATGCAT 1 cut(s) 985
NspI RCATGY 1 cut(s) 563
PagI TCATGA 1 cut(s) 1021
PceI AGGCCT 1 cut(s) 805
PciSI GCTCTTC 1 cut(s) 572
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 2 cut(s) 256, 1137
PfeI GAWTC 3 cut(s) 290, 546, 1079
PfoI TCCNGGA 1 cut(s) 210
PinAI ACCGGT 1 cut(s) 416
PkrI GCNGC 4 cut(s) 79, 128, 558, 672
PleI GAGTC 3 cut(s) 103, 154, 1370
PpsI GAGTC 3 cut(s) 103, 154, 1370
PpuMI RGGWCCY 1 cut(s) 339
Psp124BI GAGCTC 3 cut(s) 138, 476, 1317
Psp5II RGGWCCY 1 cut(s) 339
Psp6I CCWGG 2 cut(s) 210, 467
PspFI CCCAGC 1 cut(s) 1350
PspGI CCWGG 2 cut(s) 210, 467
PspN4I GGNNCC 4 cut(s) 340, 541, 696, 891
PspPI GGNCC 2 cut(s) 197, 339
PspPPI RGGWCCY 1 cut(s) 339
PsuI RGATCY 1 cut(s) 815
RsaI GTAC 5 cut(s) 86, 388, 696, 1227, 1263
RsaNI GTAC 5 cut(s) 85, 387, 695, 1226, 1262
SacI GAGCTC 3 cut(s) 138, 476, 1317
SapI GCTCTTC 1 cut(s) 572
SaqAI TTAA 7 cut(s) 188, 429, 1143, 1158, 1239, 1304, 1368
SatI GCNGC 4 cut(s) 78, 127, 557, 671
Sau3AI GATC 4 cut(s) 815, 1018, 1331, 1391
Sau96I GGNCC 2 cut(s) 197, 339
SchI GAGTC 3 cut(s) 103, 155, 1370
ScrFI CCNGG 3 cut(s) 212, 317, 469
SduI GDGCHC 7 cut(s) 138, 287, 476, 793, 920, 1317, 1472
SfaNI GCATC 3 cut(s) 64, 652, 992
SinI GGWCC 2 cut(s) 197, 339
SmlI CTYRAG 1 cut(s) 1458
SmoI CTYRAG 1 cut(s) 1458
SseBI AGGCCT 1 cut(s) 805
SsiI CCGC 5 cut(s) 127, 150, 164, 1061, 1274
SspI AATATT 1 cut(s) 1189
SspMI CTAG 4 cut(s) 99, 632, 1044, 1176
SstI GAGCTC 3 cut(s) 138, 476, 1317
StuI AGGCCT 1 cut(s) 805
StyD4I CCNGG 3 cut(s) 210, 315, 467
TaaI ACNGT 5 cut(s) 407, 1057, 1099, 1225, 1399
TaiI ACGT 2 cut(s) 60, 222
TaqI TCGA 5 cut(s) 72, 144, 717, 847, 885
TaqII GACCGA 1 cut(s) 1490
TatI WGTACW 1 cut(s) 1261
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 3 cut(s) 290, 546, 1079
Tru1I TTAA 7 cut(s) 188, 429, 1143, 1158, 1239, 1304, 1368
Tru9I TTAA 7 cut(s) 188, 429, 1143, 1158, 1239, 1304, 1368
TscAI CASTG 3 cut(s) 1104, 1228, 1315
TseFI GTSAC 3 cut(s) 178, 407, 832
TseI GCWGC 3 cut(s) 77, 556, 670
Tsp45I GTSAC 3 cut(s) 178, 407, 832
TspDTI ATGAA 6 cut(s) 336, 344, 500, 723, 885, 984
TspRI CASTG 3 cut(s) 1104, 1228, 1315
VneI GTGCAC 1 cut(s) 1468
VpaK11BI GGWCC 2 cut(s) 197, 339
XapI RAATTY 6 cut(s) 349, 851, 879, 935, 944, 1235
XceI RCATGY 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 2 cut(s) 1228, 1325
XspI CTAG 4 cut(s) 99, 632, 1044, 1176
Zsp2I ATGCAT 1 cut(s) 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.