MD06G1068100.v1.1

Protein TPX2-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005322
Physical Location & Seq
Forward (+)
16105201 .. 16110370
5170 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1068100.v1.1.491

Sequence Viewer

Length: 1521 bp
ATGGAATTGGAGGAAGATATGGAGGTTGAGCAAGTTTTAATGGCGCAGGAAGTGGACATTGACTACGAGTACGATGCAGCTCGGTACTTCGACTTTGGTAGGCAGGAGACTCCGGCTGAGGCTCGCCGCGCCGAGCTCTGGTTCGAGTCCGCCAAGAGCTACCCGCCGTCTCCTGTTGTGACAAGGTTAATACTTGGACTGGAAACCCTCCTGGAAAATGTAAACACCTTGCCGAAATCCAATGCGGAGACTGCAAATGACAGCAATTGTGATATCGGTGTGGGCACAAAAGCATGTGCAATGGATGAAAATAGCAGAGCTGAAGGAATGAATAGAGGGATCTTTGGAAATTTACAAAAAGTTTTAAATCAACCAAATGGAGCTGGTACAGGGGTGACAGTCAACAGTTACTTAAACGGTGATAAGCTTAAGGGTAAAAGTAATGCTTCTGTAAAGCCATCTTTCCCCAGGAGCTCAACTTTGATGAAGCCTACTGCAAGTCAGCTCGCTAAGCAAAATCTGAAGTCTCTAAATGGCGGTTCCAGATTCCAGATGCTGCATGTTCAAAATAATGAGAAGAGCTTATGTAGTTCTTCTGGGGTTGAAAGTCAAGCTCCCAAAAGACAGAAGCTAGATGGAGGACACTTGCGTAAGGTTACTGATGTGAAGGAGCAAACTAATTTCATCCACAAGGTGCCTAAAAAGGATGAAACTTTTGACAAAACCACTGCACATGCTAAGTTGAGACTTACTATTTCAAGAGAGCCTGACTTTGAAACGGCACATAGGGCACACAGGATTAGGCCTAACAATGGATCAATGTTAGAACAAGTGACATCAACTCATCGAAAATTCAAAGCTCGTCCATTGAACCGAAAAATTTTTGAGGCTCCTTCATTGCCACTCCCAACAAGGAGCACTCCAAAGTTGCCAGATTTTCAAGAGTTTCACCTGAAGACAATGGAAAGGGCTATGCAAAATGCATCGGCTGTTTCATCATCCTCACATCATCTCAACGATCATGACAAGGATTTGGAAAAACCAAGTATTTCTACCGTCGTGGAAAACAGAAAAGGAGAATCCAGAAGACCAAGTACAGTGGAATGTCCTAAGCAAGATAGAAATACAGGAATGCAAACATTTAAAGCTCGTCCTCTTAACAAAAGGATATTTTCTAGCAAAGGGGATATTGGTGTTTTCTGGAATAGTAAGCGGGAAACCACTGTATCAATGGAATTTAATTTTCAGACAGACAGGGGAGTACAGCAATTTCCGCCAACTGAATTTTTTAGTAAGCTCTCATTGACAACTGAGCTCCAGCAGAATAATGGATCACCAGTGAAACTTTCCCAGCCTACATCTTTGTCTACGAAGGACTCAAAAGAAAATAGATCGACTTTTTTGCAACAAGAACGTGAGATAAAAGAAAAACCTTCTCTGTTTGGTGGAAAGCAGGCTCAAGACGGGTGCATGACGGATGCTGATAAGAAACTGAGGATGAGGAGGTTGGGCGTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2_importin

Protein Analysis

507

Amino Acids

56.84

Weight (kDa)

9.35

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2_importin PF12214 248 - 415 2.4e-43 Cell cycle regulated microtubule associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 694
AccI GTMKAC 1 cut(s) 1367
AccII CGCG 1 cut(s) 129
AciI CCGC 7 cut(s) 127, 150, 164, 245, 537, 1213, 1274
AclWI GGATC 3 cut(s) 347, 823, 1339
AcsI RAATTY 5 cut(s) 349, 851, 879, 1235, 1283
AcuI CTGAAG 3 cut(s) 342, 542, 974
AcyI GRCGYC 1 cut(s) 1512
AdeI CACNNNGTG 1 cut(s) 694
AfaI GTAC 5 cut(s) 71, 86, 388, 1096, 1263
AfiI CCNNNNNNNGG 2 cut(s) 138, 812
AflII CTTAAG 1 cut(s) 428
AgsI TTSAA 7 cut(s) 566, 605, 759, 776, 856, 871, 941
AjnI CCWGG 2 cut(s) 210, 467
Alw21I GWGCWC 4 cut(s) 138, 476, 920, 1317
Alw26I GTCTC 5 cut(s) 101, 174, 242, 531, 739
AlwI GGATC 3 cut(s) 347, 823, 1339
AlwNI CAGNNNCTG 1 cut(s) 556
AoxI GGCC 1 cut(s) 803
ApeKI GCWGC 2 cut(s) 77, 556
ApoI RAATTY 5 cut(s) 349, 851, 879, 1235, 1283
AspLEI GCGC 2 cut(s) 46, 131
AsuHPI GGTGA 4 cut(s) 406, 431, 941, 1326
BaeGI GKGCMC 2 cut(s) 287, 793
BaeI ACNNNNGTAYC 4 cut(s) 378, 411, 1209, 1242
BanI GGYRCC 1 cut(s) 694
BanII GRGCYC 3 cut(s) 138, 476, 1317
BarI GAAGNNNNNNTAC 2 cut(s) 1078, 1110
BbsI GAAGAC 2 cut(s) 962, 1093
Bbv12I GWGCWC 4 cut(s) 138, 476, 920, 1317
BbvCI CCTCAGC 1 cut(s) 117
BbvI GCAGC 2 cut(s) 89, 543
BccI CCATC 2 cut(s) 466, 629
BceAI ACGGC 2 cut(s) 151, 795
BcgI CGANNNNNNTGC 4 cut(s) 56, 90, 1384, 1418
BciT130I CCWGG 2 cut(s) 212, 469
BcoDI GTCTC 5 cut(s) 101, 174, 242, 531, 739
BfaI CTAG 2 cut(s) 632, 1176
BfrI CTTAAG 1 cut(s) 428
BisI GCNGC 3 cut(s) 78, 127, 557
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 3 cut(s) 79, 128, 558
Bme1390I CCNGG 2 cut(s) 212, 469
BmiI GGNNCC 3 cut(s) 541, 696, 891
BmrFI CCNGG 2 cut(s) 212, 469
BmsI GCATC 4 cut(s) 64, 543, 992, 1468
BpiI GAAGAC 2 cut(s) 962, 1093
BpmI CTGGAG 1 cut(s) 1301
Bpu10I CCTNAGC 2 cut(s) 117, 1110
Bpu1102I GCTNAGC 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 1443
BsaHI GRCGYC 1 cut(s) 1512
BsaJI CCNNGG 1 cut(s) 467
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 812
Bse1I ACTGG 2 cut(s) 204, 1337
Bse3DI GCAATG 2 cut(s) 306, 896
BseBI CCWGG 2 cut(s) 212, 469
BseDI CCNNGG 1 cut(s) 467
BseGI GGATG 6 cut(s) 310, 684, 712, 998, 1483, 1503
BseLI CCNNNNNNNGG 2 cut(s) 138, 812
BseMI GCAATG 2 cut(s) 306, 896
BseMII CTCAG 3 cut(s) 108, 1302, 1484
BseNI ACTGG 2 cut(s) 204, 1337
BseRI GAGGAG 1 cut(s) 1516
BseSI GKGCMC 2 cut(s) 287, 793
BseXI GCAGC 2 cut(s) 89, 543
BseYI CCCAGC 1 cut(s) 1350
BsgI GTGCAG 1 cut(s) 714
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 1 cut(s) 805
BshNI GGYRCC 1 cut(s) 694
BsiHKAI GWGCWC 4 cut(s) 138, 476, 920, 1317
BsiSI CCGG 1 cut(s) 113
BslI CCNNNNNNNGG 2 cut(s) 138, 812
BsmAI GTCTC 5 cut(s) 101, 174, 242, 531, 739
BsmBI CGTCTC 1 cut(s) 174
BsmI GAATGC 1 cut(s) 1137
BsnI GGCC 1 cut(s) 805
Bsp1286I GDGCHC 6 cut(s) 138, 287, 476, 793, 920, 1317
Bsp143I GATC 5 cut(s) 339, 815, 1018, 1331, 1391
Bsp1720I GCTNAGC 1 cut(s) 510
BspACI CCGC 7 cut(s) 127, 150, 164, 245, 537, 1213, 1274
BspANI GGCC 1 cut(s) 805
BspCNI CTCAG 3 cut(s) 109, 1303, 1485
BspFNI CGCG 1 cut(s) 129
BspHI TCATGA 1 cut(s) 1021
BspLI GGNNCC 3 cut(s) 541, 696, 891
BspPI GGATC 3 cut(s) 347, 823, 1339
BspQI GCTCTTC 1 cut(s) 572
BspT107I GGYRCC 1 cut(s) 694
BspTI CTTAAG 1 cut(s) 428
BsrDI GCAATG 2 cut(s) 306, 896
BsrI ACTGG 2 cut(s) 204, 1337
BssECI CCNNGG 1 cut(s) 467
BssMI GATC 5 cut(s) 339, 815, 1018, 1331, 1391
BssNI GRCGYC 1 cut(s) 1512
Bst2UI CCWGG 2 cut(s) 212, 469
Bst4CI ACNGT 6 cut(s) 400, 407, 419, 1057, 1099, 1225
Bst6I CTCTTC 1 cut(s) 572
BstACI GRCGYC 1 cut(s) 1512
BstAFI CTTAAG 1 cut(s) 428
BstC8I GCNNGC 3 cut(s) 124, 507, 1455
BstDEI CTNAG 6 cut(s) 117, 510, 738, 1110, 1311, 1493
BstF5I GGATG 6 cut(s) 310, 684, 712, 998, 1483, 1503
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 2 cut(s) 46, 131
BstKTI GATC 5 cut(s) 342, 818, 1021, 1334, 1394
BstMAI GTCTC 5 cut(s) 101, 174, 242, 531, 739
BstMBI GATC 5 cut(s) 339, 815, 1018, 1331, 1391
BstMWI GCNNNNNNNGC 5 cut(s) 128, 251, 511, 788, 1273
BstNI CCWGG 2 cut(s) 212, 469
BstNSI RCATGY 3 cut(s) 297, 563, 737
BstSCI CCNGG 2 cut(s) 210, 467
BstSLI GKGCMC 2 cut(s) 287, 793
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 2 cut(s) 89, 543
BstV2I GAAGAC 2 cut(s) 962, 1093
BstX2I RGATCY 1 cut(s) 339
BstYI RGATCY 1 cut(s) 339
BsuRI GGCC 1 cut(s) 805
BtsCI GGATG 6 cut(s) 310, 684, 712, 998, 1483, 1503
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 4 cut(s) 726, 1104, 1221, 1344
Cac8I GCNNGC 3 cut(s) 124, 507, 1455
CaiI CAGNNNCTG 1 cut(s) 556
CciI TCATGA 1 cut(s) 1021
CfoI GCGC 2 cut(s) 46, 131
CseI GACGC 1 cut(s) 1501
Csp6I GTAC 5 cut(s) 70, 85, 387, 1095, 1262
CspCI CAANNNNNGTGG 2 cut(s) 1080, 1115
CviAII CATG 5 cut(s) 294, 560, 734, 1022, 1471
CviQI GTAC 5 cut(s) 70, 85, 387, 1095, 1262
DdeI CTNAG 6 cut(s) 117, 510, 738, 1110, 1311, 1493
DpnI GATC 5 cut(s) 341, 817, 1020, 1333, 1393
DpnII GATC 5 cut(s) 339, 815, 1018, 1331, 1391
DraI TTTAAA 2 cut(s) 366, 1144
DraIII CACNNNGTG 1 cut(s) 694
Eam1104I CTCTTC 1 cut(s) 572
EarI CTCTTC 1 cut(s) 572
EciI GGCGGA 2 cut(s) 139, 1263
Ecl136II GAGCTC 3 cut(s) 136, 474, 1315
Eco147I AGGCCT 1 cut(s) 805
Eco24I GRGCYC 3 cut(s) 138, 476, 1317
Eco32I GATATC 1 cut(s) 274
Eco53kI GAGCTC 3 cut(s) 136, 474, 1315
Eco57I CTGAAG 3 cut(s) 342, 542, 974
EcoICRI GAGCTC 3 cut(s) 136, 474, 1315
EcoRII CCWGG 2 cut(s) 210, 467
EcoRV GATATC 1 cut(s) 274
EcoT22I ATGCAT 1 cut(s) 985
EcoT38I GRGCYC 3 cut(s) 138, 476, 1317
Esp3I CGTCTC 1 cut(s) 174
FaeI CATG 5 cut(s) 297, 563, 737, 1025, 1474
FaiI YATR 9 cut(s) 20, 295, 561, 586, 735, 786, 974, 1023, 1472
FalI AAGNNNNNCTT 2 cut(s) 430, 462
FatI CATG 5 cut(s) 293, 559, 733, 1021, 1470
FauI CCCGC 2 cut(s) 171, 1206
FblI GTMKAC 1 cut(s) 1367
Fnu4HI GCNGC 3 cut(s) 78, 127, 557
FokI GGATG 6 cut(s) 317, 671, 719, 985, 1490, 1510
FriOI GRGCYC 3 cut(s) 138, 476, 1317
Fsp4HI GCNGC 3 cut(s) 78, 127, 557
FspBI CTAG 2 cut(s) 632, 1176
GlaI GCGC 2 cut(s) 45, 130
GluI GCNGC 3 cut(s) 78, 127, 557
GsaI CCCAGC 1 cut(s) 1354
GsuI CTGGAG 1 cut(s) 1301
HaeIII GGCC 1 cut(s) 805
HapII CCGG 1 cut(s) 113
HgaI GACGC 1 cut(s) 1501
HhaI GCGC 2 cut(s) 46, 131
Hin1I GRCGYC 1 cut(s) 1512
Hin1II CATG 5 cut(s) 297, 563, 737, 1025, 1474
Hin6I GCGC 2 cut(s) 44, 129
HinP1I GCGC 2 cut(s) 44, 129
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 5 cut(s) 109, 146, 546, 1079, 1376
HpaII CCGG 1 cut(s) 113
HphI GGTGA 4 cut(s) 406, 431, 941, 1326
Hpy166II GTNNAC 4 cut(s) 55, 223, 403, 1368
Hpy188I TCNGA 2 cut(s) 522, 1248
Hpy188III TCNNGA 8 cut(s) 543, 550, 759, 941, 1022, 1083, 1201, 1460
Hpy8I GTNNAC 4 cut(s) 55, 223, 403, 1368
Hpy99I CGWCG 1 cut(s) 1061
HpyAV CCTTC 5 cut(s) 317, 661, 903, 1366, 1443
HpyCH4III ACNGT 6 cut(s) 400, 407, 419, 1057, 1099, 1225
HpyCH4IV ACGT 1 cut(s) 1414
HpyF10VI GCNNNNNNNGC 5 cut(s) 128, 251, 511, 788, 1273
HpyF3I CTNAG 6 cut(s) 117, 510, 738, 1110, 1311, 1493
HpySE526I ACGT 1 cut(s) 1414
Hsp92I GRCGYC 1 cut(s) 1512
Hsp92II CATG 5 cut(s) 297, 563, 737, 1025, 1474
HspAI GCGC 2 cut(s) 44, 129
Kzo9I GATC 5 cut(s) 339, 815, 1018, 1331, 1391
LguI GCTCTTC 1 cut(s) 572
LmnI GCTCC 7 cut(s) 380, 471, 619, 670, 895, 915, 1320
Lsp1109I GCAGC 2 cut(s) 89, 543
LweI GCATC 4 cut(s) 64, 543, 992, 1468
MaeI CTAG 2 cut(s) 632, 1176
MaeII ACGT 1 cut(s) 1414
MaeIII GTNAC 5 cut(s) 178, 394, 407, 655, 832
MalI GATC 5 cut(s) 341, 817, 1020, 1333, 1393
MboI GATC 5 cut(s) 339, 815, 1018, 1331, 1391
MboII GAAGA 5 cut(s) 26, 585, 589, 967, 1098
MfeI CAATTG 1 cut(s) 265
MflI RGATCY 1 cut(s) 339
MhlI GDGCHC 6 cut(s) 138, 287, 476, 793, 920, 1317
MlyI GAGTC 3 cut(s) 103, 155, 1370
Mph1103I ATGCAT 1 cut(s) 985
MseI TTAA 8 cut(s) 38, 188, 365, 413, 429, 1143, 1158, 1239
MspCI CTTAAG 1 cut(s) 428
MspI CCGG 1 cut(s) 113
MspR9I CCNGG 2 cut(s) 212, 469
MunI CAATTG 1 cut(s) 265
Mva1269I GAATGC 1 cut(s) 1137
MvaI CCWGG 2 cut(s) 212, 469
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 5 cut(s) 128, 251, 511, 788, 1273
NdeII GATC 5 cut(s) 339, 815, 1018, 1331, 1391
NlaIII CATG 5 cut(s) 297, 563, 737, 1025, 1474
NlaIV GGNNCC 3 cut(s) 541, 696, 891
NmeAIII GCCGAG 1 cut(s) 157
NmuCI GTSAC 3 cut(s) 178, 394, 832
NsiI ATGCAT 1 cut(s) 985
NspI RCATGY 3 cut(s) 297, 563, 737
PagI TCATGA 1 cut(s) 1021
PceI AGGCCT 1 cut(s) 805
PciSI GCTCTTC 1 cut(s) 572
PctI GAATGC 1 cut(s) 1137
PfeI GAWTC 2 cut(s) 546, 1079
PfoI TCCNGGA 1 cut(s) 210
PkrI GCNGC 3 cut(s) 79, 128, 558
PleI GAGTC 3 cut(s) 103, 154, 1370
PpsI GAGTC 3 cut(s) 103, 154, 1370
Psp124BI GAGCTC 3 cut(s) 138, 476, 1317
Psp6I CCWGG 2 cut(s) 210, 467
PspFI CCCAGC 1 cut(s) 1350
PspGI CCWGG 2 cut(s) 210, 467
PspN4I GGNNCC 3 cut(s) 541, 696, 891
PstNI CAGNNNCTG 1 cut(s) 556
PsuI RGATCY 1 cut(s) 339
RsaI GTAC 5 cut(s) 71, 86, 388, 1096, 1263
RsaNI GTAC 5 cut(s) 70, 85, 387, 1095, 1262
SacI GAGCTC 3 cut(s) 138, 476, 1317
SapI GCTCTTC 1 cut(s) 572
SaqAI TTAA 8 cut(s) 38, 188, 365, 413, 429, 1143, 1158, 1239
SatI GCNGC 3 cut(s) 78, 127, 557
Sau3AI GATC 5 cut(s) 339, 815, 1018, 1331, 1391
SchI GAGTC 3 cut(s) 103, 155, 1370
ScrFI CCNGG 2 cut(s) 212, 469
SduI GDGCHC 6 cut(s) 138, 287, 476, 793, 920, 1317
SfaNI GCATC 4 cut(s) 64, 543, 992, 1468
SmlI CTYRAG 2 cut(s) 428, 1458
SmoI CTYRAG 2 cut(s) 428, 1458
SseBI AGGCCT 1 cut(s) 805
SsiI CCGC 7 cut(s) 127, 150, 164, 245, 537, 1213, 1274
SspMI CTAG 2 cut(s) 632, 1176
SstI GAGCTC 3 cut(s) 138, 476, 1317
StuI AGGCCT 1 cut(s) 805
StyD4I CCNGG 2 cut(s) 210, 467
TaaI ACNGT 6 cut(s) 400, 407, 419, 1057, 1099, 1225
TaiI ACGT 1 cut(s) 1417
TaqI TCGA 4 cut(s) 90, 144, 847, 1394
TatI WGTACW 2 cut(s) 1094, 1261
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 2 cut(s) 546, 1079
Tru1I TTAA 8 cut(s) 38, 188, 365, 413, 429, 1143, 1158, 1239
Tru9I TTAA 8 cut(s) 38, 188, 365, 413, 429, 1143, 1158, 1239
TscAI CASTG 4 cut(s) 733, 1104, 1228, 1344
TseFI GTSAC 3 cut(s) 178, 394, 832
TseI GCWGC 2 cut(s) 77, 556
Tsp45I GTSAC 3 cut(s) 178, 394, 832
TspDTI ATGAA 7 cut(s) 321, 344, 500, 673, 723, 885, 984
TspGWI ACGGA 1 cut(s) 1490
TspRI CASTG 4 cut(s) 733, 1104, 1228, 1344
Vha464I CTTAAG 1 cut(s) 428
XapI RAATTY 5 cut(s) 349, 851, 879, 1235, 1283
XceI RCATGY 3 cut(s) 297, 563, 737
XcmI CCANNNNNNNNNTGG 2 cut(s) 1228, 1325
XmiI GTMKAC 1 cut(s) 1367
XspI CTAG 2 cut(s) 632, 1176
Zsp2I ATGCAT 1 cut(s) 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.