Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005582

Physical Location & Seq

Genomic Coordinates Reverse (-)

12342376 .. 12346051

Gene Structure 3676 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G23700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2463 bp

ATGGGAACAAACGGTACAACATGTCCAGGACCAATGAAAGCAACCTCTAATGGAGTCTTTCAAGGAGAGAATCCTCTGGAACACGCGTTGCCTCTTCTCATACTCCAGATCTGTATCGTTCTTCTTCTCACTCGTCTTCTTGCTTTTCTCCTCCGTCCTCTCCGGCAGCCACGTGTCATCGCCGAGATTGTCGGTGGAATATTACTTGGACCATCAGCACTTGGGAAAAGCACTAAGTTTATCAACACAGTTTTCCCACCAAAGAGTCTAACGGTTTTAGACACACTTGCAAACTTAGGACTCATCTTCTTCCTCTTCCTCGTTGGTCTCGAGCTCGATCCCAAATCTCTTAAGCGAACCGGAAAGAGAGCTCTCTCTATCGCTCTCGCCGGAATCACTCTTCCTTTCGTATTAGGCATTGGAACTTCTTTTGCTCTCCGCAGTTCCATAGCTGATGGAGCCAGCAAAGCTCCTTTCCTTGTCTTCATGGGTGTTGCTCTCTCCATCACAGCGTTTCCCGTCTTGGCCCGAATACTCGCTGAGATTAAGCTTCTAACCACTGATATCGGAAAGATTGCTTTGTCCGCTGCTGCGGTCAATGACGTGGCTGCTTGGATTCTCCTTGCTCTCGCGGTGGCTCTTTCTGGAGAGGGGAGCTCTCCGCTTACTTCTCTTTGGGTGTTTCTCTCGGGTTGCGGTTTTGTCTTGTTCTGTATCTTTGTTGTGCAACCAGGGATTAAGTTGATTGCTAAACGTTGCCCTGAAGGAGAACCGGTAAATGAACTTTACGTGTGTTGCACGTTAGGTATTGTTCTTGCAGCTAGCTTCGTCACAGACTTTATTGGGATTCATGCGCTGTTTGGTGCTTTTGTGATCGGGGTTATTTTCCCTAAAGAAGGCAATTTCGCGAATGCTTTAGTTGAGAAAGTTGAGGATCTCGTGTCAGGTCTTTTCTTGCCGCTCTACTTCGTCTCAAGTGGTTTAAAGACCAATGTTGCGACGATCCAAGGAGCTCAGTCTTGGGGACTCTTGGTTTTGGTTATATTTAATGCTTGTTTCGGTAAAATCATCGGTACAGTCCTAGTTTCCCTTTACTGCAAAGTTCCGCTTGACCAGTCTTTAGCGCTTGGTTTCTTGATGAACACGAAAGGTCTTGTTGAGCTCATCGTCCTCAACATTGGTAAAGACAGAGGGGTTCTGAATGATCAAATTTTCGCTATAATGGTTCTAATGGCGATATTCACCACATTCATGACGACTCCTCTAGTTCTGGCAGTGTACAAACCGGGTAAATCCTTAACCAAAGCTGATTATAAGAACCGAACGGTGGAGGAGACGAACCGTTCAAACAAACCGCTCTGCCTCATGTTCTGTTTCCAGAGCATAATGAACATCCCCACGATCGTCAACCTCATAGAAGCATCCCGAGGCATAAACCGTAAAGAAAATCTATCAGTCTACGCGATGCATCTTATGGAGCTTTCAGAGAGATCATCGGCTATACTCATGGCACACAAGGTCAGGAGAAACGGTCTTCCCTTCTGGAACAAAGACAAATCTGAGAACAATAGTTCTTCTTCCGACATGGTTGTGGTCGCATTCGAGGCTTTCCGAAGACTCAGCCGTGTCTCAGTGCGTCCCATGACAGCGATCTCACCGATGGCGACTATACATGAGGATATTTGCCAAAGCGCGGAACGCAAAAAAACCGCTATGGTGATTCTTCCGTTTCATAAGCACGTTAGGCTGGACAGAACATGGGAGACGACTAGGAATGACTACCGTTGGATTAACAAGAAGGTTATGGAGGAATCTCCATGTTCTGTTGCGATTTTGGTTGATCGTGGACTTGGTGGTACGACACGTGTCGCGTCTAGTGATTTCTCGTTGACTATAACAGTTTTGTTTTTTGGAGGGAATGATGATCGTGAGGCGTTAGCGTTTGCGGTGCGAATGGCTGAACATCCCGGGATAAGCTTAACCGTTGTTCGGTTTATCCCTAGCGATGAATTCAAACCGGAAAACGTGAGGATCGAGATAACCGAAGATCAGCTCTGTTCAGGGGCAACTAGATTGATTGATATTGAAGCTATAACCGAACTTAAAGCAAAGATTAAAGAAAAAGAAAGTTCTCGTTCTAATTCGGATTCTGAATCTCATATCATTTACGAAGAGAAAATCGTGAAATGTTACGAGGAAGTTATCGAAGTTATTAAAGAGTATTCTAAAAGCAACCTTTTCTTGGTGGGGAAGTCACCGGAAGGTTCTGTAGCGTCAGGGATAAACGTAAGAAGCGACACGCCTGAGCTTGGACCGATCGGGAATTTGTTAACGGAAAGCGAAAGTGTTTCGACGGTGGCATCAGTGTTGGTGGTGCAACAGTATATAGCAAGCCGTCCGGTCGGAATTTCGAAGAACGTCACGACTGAAGAGTCGCTGGTTGAGGATTCTGAGAGTCCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005783 GO:0006605 GO:0006623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0012505 GO:0015031 GO:0015672 GO:0015833 GO:0031410 GO:0031982 GO:0033036 GO:0033365 GO:0034613 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0097708 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

820

Amino Acids

89.16

Weight (kDa)

8.06

Isoelectric Point (pI)

39.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	42 - 423	1.6e-65	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	483 - 625	1.5e-44	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	629 - 796	2e-29	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005582)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G23700 AT4G23700
fragaria_vesca	FvH4_5g03731 FvH4_5g03731
malus_domestica	MD00G1094100.v1.1 MD06G1097000.v1.1
prunus_persica	Prupe.5G111700_v2.0.a1 Prupe.5G111700_v2.0.a1 Prupe.5G111700_v2.0.a1 Prupe.5G111700_v2.0.a1 Prupe.5G111700_v2.0.a1 Prupe.5G111700_v2.0.a1
pyrus_communis	pycom06g09310
rosa_chinensis	RchiOBHm_Chr7g0195971
rosa_laevigata	RLG00000004078
rosa_multiflora	Rmu_co8262837.1_g000001 Rmu_sc0004016.1_g000001 Rmu_ssc0000453.1_g000003
rosa_roxburghii	Rroxscaffold_3G00259980
rosa_rugosa	Rorug07G0027700 Rorug07G0027900 Rorug07G0028000 Rorug07G0028100
rosa_samantha	Rh7AG155200 Rh7BG157100 Rh7CG160600 Rh7DG156100
rosa_wichuraiana	Rw7G013270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1314
AasI	GACNNNNNNGTC	1 cut(s)	2431
AccBSI	CCGCTC	2 cut(s)	961, 1357
AccI	GTMKAC	1 cut(s)	1458
AccII	CGCG	6 cut(s)	86, 632, 908, 1463, 1694, 1871
AclI	AACGTT	1 cut(s)	754
AclWI	GGATC	4 cut(s)	332, 942, 997, 2039
AcsI	RAATTY	4 cut(s)	1209, 2009, 2323, 2406
AcuI	CTGAAG	2 cut(s)	783, 2448
AcvI	CACGTG	2 cut(s)	173, 1865
AfaI	GTAC	4 cut(s)	16, 1075, 1280, 1858
AfeI	AGCGCT	1 cut(s)	1125
AfiI	CCNNNNNNNGG	6 cut(s)	896, 1327, 1693, 1989, 2242, 2309
AflII	CTTAAG	1 cut(s)	350
AflIII	ACRYGT	6 cut(s)	20, 84, 172, 789, 1862, 1864
AgeI	ACCGGT	1 cut(s)	772
AgsI	TTSAA	4 cut(s)	62, 1347, 2014, 2087
AjiI	CACGTC	1 cut(s)	604
AjnI	CCWGG	2 cut(s)	25, 730
Alw21I	GWGCWC	5 cut(s)	336, 373, 659, 1015, 1164
Alw26I	GTCTC	5 cut(s)	332, 976, 1328, 1633, 1757
AlwI	GGATC	4 cut(s)	332, 942, 997, 2039
Ama87I	CYCGRG	4 cut(s)	329, 688, 1425, 1967
Aor51HI	AGCGCT	1 cut(s)	1125
AoxI	GGCC	1 cut(s)	525
ApeKI	GCWGC	5 cut(s)	166, 587, 590, 608, 818
ApoI	RAATTY	4 cut(s)	1209, 2009, 2323, 2406
AsiGI	ACCGGT	1 cut(s)	772
AspLEI	GCGC	3 cut(s)	856, 1126, 1694
AspS9I	GGNCC	4 cut(s)	29, 209, 526, 2312
AsuC2I	CCSGG	3 cut(s)	1287, 1968, 1969
AsuHPI	GGTGA	4 cut(s)	1234, 1647, 1729, 2247
AsuII	TTCGAA	1 cut(s)	2411
AsuNHI	GCTAGC	1 cut(s)	821
AvaI	CYCGRG	4 cut(s)	329, 688, 1425, 1967
AvaII	GGWCC	3 cut(s)	29, 209, 2312
BanII	GRGCYC	5 cut(s)	336, 373, 659, 1015, 1164
BauI	CACGAG	1 cut(s)	938
BbrPI	CACGTG	2 cut(s)	173, 1865
BbsI	GAAGAC	4 cut(s)	128, 475, 1526, 1621
Bbv12I	GWGCWC	5 cut(s)	336, 373, 659, 1015, 1164
BbvI	GCAGC	5 cut(s)	178, 574, 577, 595, 830
BccI	CCATC	4 cut(s)	220, 449, 512, 1654
BceAI	ACGGC	2 cut(s)	1608, 2379
BciT130I	CCWGG	2 cut(s)	27, 732
BclI	TGATCA	1 cut(s)	1204
BcnI	CCSGG	3 cut(s)	1287, 1968, 1969
BcoDI	GTCTC	5 cut(s)	332, 976, 1328, 1633, 1757
BfaI	CTAG	7 cut(s)	822, 1082, 1265, 1770, 1875, 2001, 2070
BfmI	CTRYAG	1 cut(s)	2268
BfoI	RGCGCY	1 cut(s)	1127
BfrI	CTTAAG	1 cut(s)	350
BglII	AGATCT	1 cut(s)	108
BisI	GCNGC	6 cut(s)	167, 588, 591, 609, 819, 959
BlsI	GCNGC	6 cut(s)	168, 589, 592, 610, 820, 960
Bme1390I	CCNGG	5 cut(s)	27, 732, 1287, 1968, 1969
Bme18I	GGWCC	3 cut(s)	29, 209, 2312
BmeT110I	CYCGRG	4 cut(s)	329, 688, 1425, 1967
BmgBI	CACGTC	1 cut(s)	604
BmgT120I	GGNCC	4 cut(s)	29, 209, 526, 2312
BmiI	GGNNCC	1 cut(s)	460
BmrFI	CCNGG	5 cut(s)	27, 732, 1287, 1968, 1969
BmsI	GCATC	4 cut(s)	1430, 1455, 1477, 2369
BmtI	GCTAGC	1 cut(s)	825
BoxI	GACNNNNGTC	1 cut(s)	1865
BpiI	GAAGAC	4 cut(s)	128, 475, 1526, 1621
BplI	GAGNNNNNCTC	2 cut(s)	641, 673
BpmI	CTGGAG	2 cut(s)	89, 666
Bpu10I	CCTNAGC	1 cut(s)	2304
Bpu14I	TTCGAA	1 cut(s)	2411
BpuEI	CTTGAG	1 cut(s)	958
BpuMI	CCSGG	3 cut(s)	1287, 1968, 1969
BsaAI	YACGTR	3 cut(s)	173, 790, 1865
BsaBI	GATNNNNATC	1 cut(s)	113
BsaI	GGTCTC	1 cut(s)	332
BsaJI	CCNNGG	4 cut(s)	731, 1006, 1426, 1967
BsaWI	WCCGGW	5 cut(s)	359, 772, 2017, 2257, 2398
BsaXI	ACNNNNNCTCC	4 cut(s)	759, 789, 1516, 1546
Bsc4I	CCNNNNNNNGG	6 cut(s)	896, 1327, 1693, 1989, 2242, 2309
Bse118I	RCCGGY	1 cut(s)	772
Bse1I	ACTGG	1 cut(s)	1114
Bse8I	GATNNNNATC	1 cut(s)	113
BseBI	CCWGG	2 cut(s)	27, 732
BseDI	CCNNGG	4 cut(s)	731, 1006, 1426, 1967
BseGI	GGATG	3 cut(s)	1392, 1421, 1963
BseJI	GATNNNNATC	1 cut(s)	113
BseLI	CCNNNNNNNGG	6 cut(s)	896, 1327, 1693, 1989, 2242, 2309
BseMII	CTCAG	7 cut(s)	531, 1028, 1551, 1633, 1644, 2295, 2442
BseNI	ACTGG	1 cut(s)	1114
BseRI	GAGGAG	3 cut(s)	140, 1251, 1346
BseXI	GCAGC	5 cut(s)	178, 574, 577, 595, 830
Bsh1236I	CGCG	6 cut(s)	86, 632, 908, 1463, 1694, 1871
Bsh1285I	CGRYCG	3 cut(s)	1404, 2319, 2403
BshFI	GGCC	1 cut(s)	527
BshTI	ACCGGT	1 cut(s)	772
BsiEI	CGRYCG	3 cut(s)	1404, 2319, 2403
BsiHKAI	GWGCWC	5 cut(s)	336, 373, 659, 1015, 1164
BsiHKCI	CYCGRG	4 cut(s)	329, 688, 1425, 1967
BsiSI	CCGG	9 cut(s)	163, 360, 390, 773, 1286, 1968, 2018, 2258, 2399
BslFI	GGGAC	2 cut(s)	1038, 1623
BslI	CCNNNNNNNGG	6 cut(s)	896, 1327, 1693, 1989, 2242, 2309
BsmAI	GTCTC	5 cut(s)	332, 976, 1328, 1633, 1757
BsmBI	CGTCTC	3 cut(s)	976, 1328, 1757
BsmFI	GGGAC	2 cut(s)	1038, 1623
BsmI	GAATGC	2 cut(s)	916, 1598
BsnI	GGCC	1 cut(s)	527
Bso31I	GGTCTC	1 cut(s)	332
BsoBI	CYCGRG	4 cut(s)	329, 688, 1425, 1967
Bsp119I	TTCGAA	1 cut(s)	2411
Bsp1286I	GDGCHC	5 cut(s)	336, 373, 659, 1015, 1164
Bsp1407I	TGTACA	1 cut(s)	1278
Bsp68I	TCGCGA	1 cut(s)	908
BspANI	GGCC	1 cut(s)	527
BspCNI	CTCAG	7 cut(s)	532, 1027, 1552, 1632, 1643, 2296, 2443
BspFNI	CGCG	6 cut(s)	86, 632, 908, 1463, 1694, 1871
BspHI	TCATGA	1 cut(s)	1251
BspLI	GGNNCC	1 cut(s)	460
BspOI	GCTAGC	1 cut(s)	825
BspPI	GGATC	4 cut(s)	332, 942, 997, 2039
BspT104I	TTCGAA	1 cut(s)	2411
BspTI	CTTAAG	1 cut(s)	350
BspTNI	GGTCTC	1 cut(s)	332
BsrBI	CCGCTC	2 cut(s)	961, 1357
BsrFI	RCCGGY	1 cut(s)	772
BsrGI	TGTACA	1 cut(s)	1278
BsrI	ACTGG	1 cut(s)	1114
BssAI	RCCGGY	1 cut(s)	772
BssECI	CCNNGG	4 cut(s)	731, 1006, 1426, 1967
BssSI	CACGAG	1 cut(s)	938
BssT1I	CCWWGG	1 cut(s)	1006
Bst2BI	CACGAG	1 cut(s)	938
Bst2UI	CCWGG	2 cut(s)	27, 732
Bst6I	CTCTTC	5 cut(s)	99, 320, 405, 2166, 2424
BstAFI	CTTAAG	1 cut(s)	350
BstAUI	TGTACA	1 cut(s)	1278
BstBAI	YACGTR	3 cut(s)	173, 790, 1865
BstBI	TTCGAA	1 cut(s)	2411
BstC8I	GCNNGC	3 cut(s)	463, 823, 2392
BstENI	CCTNNNNNAGG	1 cut(s)	894
BstF5I	GGATG	3 cut(s)	1392, 1421, 1963
BstFNI	CGCG	6 cut(s)	86, 632, 908, 1463, 1694, 1871
BstH2I	RGCGCY	1 cut(s)	1127
BstHHI	GCGC	3 cut(s)	856, 1126, 1694
BstMAI	GTCTC	5 cut(s)	332, 976, 1328, 1633, 1757
BstMCI	CGRYCG	3 cut(s)	1404, 2319, 2403
BstMWI	GCNNNNNNNGC	6 cut(s)	458, 467, 584, 1604, 1698, 1744
BstNI	CCWGG	2 cut(s)	27, 732
BstNSI	RCATGY	1 cut(s)	24
BstPAI	GACNNNNGTC	1 cut(s)	1865
BstSCI	CCNGG	5 cut(s)	25, 730, 1285, 1966, 1967
BstSFI	CTRYAG	1 cut(s)	2268
BstUI	CGCG	6 cut(s)	86, 632, 908, 1463, 1694, 1871
BstV1I	GCAGC	5 cut(s)	178, 574, 577, 595, 830
BstV2I	GAAGAC	4 cut(s)	128, 475, 1526, 1621
BstX2I	RGATCY	2 cut(s)	108, 934
BstYI	RGATCY	2 cut(s)	108, 934
BsuRI	GGCC	1 cut(s)	527
BtgZI	GCGATG	3 cut(s)	163, 1478, 2019
BtrI	CACGTC	1 cut(s)	604
BtsCI	GGATG	3 cut(s)	1392, 1421, 1963
BtsI	GCAGTG	1 cut(s)	1281
BtsIMutI	CAGTG	4 cut(s)	558, 1281, 1638, 2370
BtuMI	TCGCGA	1 cut(s)	908
Cac8I	GCNNGC	3 cut(s)	463, 823, 2392
CciI	TCATGA	1 cut(s)	1251
CfoI	GCGC	3 cut(s)	856, 1126, 1694
Cfr10I	RCCGGY	1 cut(s)	772
Cfr13I	GGNCC	4 cut(s)	29, 209, 526, 2312
Cfr9I	CCCGGG	1 cut(s)	1967
CseI	GACGC	3 cut(s)	1625, 1860, 2262
Csp6I	GTAC	4 cut(s)	15, 1074, 1279, 1857
CspAI	ACCGGT	1 cut(s)	772
CviQI	GTAC	4 cut(s)	15, 1074, 1279, 1857
DraI	TTTAAA	1 cut(s)	984
DrdI	GACNNNNNNGTC	1 cut(s)	2431
DseDI	GACNNNNNNGTC	1 cut(s)	2431
Eam1104I	CTCTTC	5 cut(s)	99, 320, 405, 2166, 2424
EarI	CTCTTC	5 cut(s)	99, 320, 405, 2166, 2424
Ecl136II	GAGCTC	5 cut(s)	334, 371, 657, 1013, 1162
Eco130I	CCWWGG	1 cut(s)	1006
Eco24I	GRGCYC	5 cut(s)	336, 373, 659, 1015, 1164
Eco31I	GGTCTC	1 cut(s)	332
Eco32I	GATATC	1 cut(s)	565
Eco47I	GGWCC	3 cut(s)	29, 209, 2312
Eco47III	AGCGCT	1 cut(s)	1125
Eco53kI	GAGCTC	5 cut(s)	334, 371, 657, 1013, 1162
Eco57I	CTGAAG	2 cut(s)	783, 2448
Eco72I	CACGTG	2 cut(s)	173, 1865
Eco88I	CYCGRG	4 cut(s)	329, 688, 1425, 1967
EcoICRI	GAGCTC	5 cut(s)	334, 371, 657, 1013, 1162
EcoNI	CCTNNNNNAGG	1 cut(s)	894
EcoRI	GAATTC	1 cut(s)	2009
EcoRII	CCWGG	2 cut(s)	25, 730
EcoRV	GATATC	1 cut(s)	565
EcoT14I	CCWWGG	1 cut(s)	1006
EcoT22I	ATGCAT	1 cut(s)	1470
EcoT38I	GRGCYC	5 cut(s)	336, 373, 659, 1015, 1164
ErhI	CCWWGG	1 cut(s)	1006
Esp3I	CGTCTC	3 cut(s)	976, 1328, 1757
FalI	AAGNNNNNCTT	2 cut(s)	1092, 1124
FaqI	GGGAC	2 cut(s)	1038, 1623
FbaI	TGATCA	1 cut(s)	1204
FblI	GTMKAC	1 cut(s)	1458
Fnu4HI	GCNGC	6 cut(s)	167, 588, 591, 609, 819, 959
FokI	GGATG	3 cut(s)	1379, 1408, 1950
FriOI	GRGCYC	5 cut(s)	336, 373, 659, 1015, 1164
Fsp4HI	GCNGC	6 cut(s)	167, 588, 591, 609, 819, 959
FspBI	CTAG	7 cut(s)	822, 1082, 1265, 1770, 1875, 2001, 2070
GlaI	GCGC	3 cut(s)	855, 1125, 1693
GluI	GCNGC	6 cut(s)	167, 588, 591, 609, 819, 959
GsuI	CTGGAG	2 cut(s)	89, 666
HaeII	RGCGCY	1 cut(s)	1127
HaeIII	GGCC	1 cut(s)	527
HapII	CCGG	9 cut(s)	163, 360, 390, 773, 1286, 1968, 2018, 2258, 2399
HgaI	GACGC	3 cut(s)	1625, 1860, 2262
HhaI	GCGC	3 cut(s)	856, 1126, 1694
Hin6I	GCGC	3 cut(s)	854, 1124, 1692
HinP1I	GCGC	3 cut(s)	854, 1124, 1692
HincII	GTYRAC	3 cut(s)	1408, 1890, 2331
HindII	GTYRAC	3 cut(s)	1408, 1890, 2331
HindIII	AAGCTT	2 cut(s)	548, 1975
HpaI	GTTAAC	1 cut(s)	2331
HpaII	CCGG	9 cut(s)	163, 360, 390, 773, 1286, 1968, 2018, 2258, 2399
HphI	GGTGA	4 cut(s)	1234, 1647, 1729, 2247
Hpy166II	GTNNAC	6 cut(s)	1279, 1408, 1459, 1847, 1890, 2331
Hpy8I	GTNNAC	6 cut(s)	1279, 1408, 1459, 1847, 1890, 2331
Hpy99I	CGWCG	2 cut(s)	1003, 2356
HpyAV	CCTTC	5 cut(s)	758, 890, 1549, 1792, 2255
HpyCH4V	TGCA	7 cut(s)	290, 727, 798, 818, 1098, 1468, 2377
HpyF10VI	GCNNNNNNNGC	6 cut(s)	458, 467, 584, 1604, 1698, 1744
HspAI	GCGC	3 cut(s)	854, 1124, 1692
Ksp22I	TGATCA	1 cut(s)	1204
KspAI	GTTAAC	1 cut(s)	2331
LmnI	GCTCC	5 cut(s)	458, 475, 654, 1010, 1477
Lsp1109I	GCAGC	5 cut(s)	178, 574, 577, 595, 830
LweI	GCATC	4 cut(s)	1430, 1455, 1477, 2369
MaeI	CTAG	7 cut(s)	822, 1082, 1265, 1770, 1875, 2001, 2070
MaeIII	GTNAC	4 cut(s)	829, 2189, 2253, 2419
MbiI	CCGCTC	2 cut(s)	961, 1357
MflI	RGATCY	2 cut(s)	108, 934
MhlI	GDGCHC	5 cut(s)	336, 373, 659, 1015, 1164
MluCI	AATT	6 cut(s)	901, 1209, 2009, 2140, 2323, 2406
MluI	ACGCGT	1 cut(s)	84
MlyI	GAGTC	7 cut(s)	63, 274, 294, 1020, 1252, 1611, 2441
MmeI	TCCRAC	3 cut(s)	1605, 1766, 2383
Mph1103I	ATGCAT	1 cut(s)	1470
MslI	CAYNNNNRTG	2 cut(s)	1250, 1589
MspA1I	CMGCKG	1 cut(s)	587
MspCI	CTTAAG	1 cut(s)	350
MspI	CCGG	9 cut(s)	163, 360, 390, 773, 1286, 1968, 2018, 2258, 2399
MspR9I	CCNGG	5 cut(s)	27, 732, 1287, 1968, 1969
Mva1269I	GAATGC	2 cut(s)	916, 1598
MvaI	CCWGG	2 cut(s)	27, 732
MvnI	CGCG	6 cut(s)	86, 632, 908, 1463, 1694, 1871
MwoI	GCNNNNNNNGC	6 cut(s)	458, 467, 584, 1604, 1698, 1744
NciI	CCSGG	3 cut(s)	1287, 1968, 1969
NheI	GCTAGC	1 cut(s)	821
NlaIV	GGNNCC	1 cut(s)	460
NmeAIII	GCCGAG	1 cut(s)	208
NmuCI	GTSAC	3 cut(s)	829, 2253, 2419
NruI	TCGCGA	1 cut(s)	908
NsiI	ATGCAT	1 cut(s)	1470
NspI	RCATGY	1 cut(s)	24
NspV	TTCGAA	1 cut(s)	2411
PaeR7I	CTCGAG	1 cut(s)	329
PagI	TCATGA	1 cut(s)	1251
PciI	ACATGT	1 cut(s)	20
PcsI	WCGNNNNNNNCGW	4 cut(s)	327, 2031, 2040, 2292
PctI	GAATGC	2 cut(s)	916, 1598
PfeI	GAWTC	9 cut(s)	70, 393, 616, 847, 1720, 1811, 2147, 2153, 2447
PfoI	TCCNGGA	1 cut(s)	25
PinAI	ACCGGT	1 cut(s)	772
PkrI	GCNGC	6 cut(s)	168, 589, 592, 610, 820, 960
Ple19I	CGATCG	2 cut(s)	1404, 2319
PleI	GAGTC	7 cut(s)	62, 273, 294, 1020, 1252, 1611, 2440
PmaCI	CACGTG	2 cut(s)	173, 1865
PmlI	CACGTG	2 cut(s)	173, 1865
PpsI	GAGTC	7 cut(s)	62, 273, 294, 1020, 1252, 1611, 2440
Ppu21I	YACGTR	3 cut(s)	173, 790, 1865
PscI	ACATGT	1 cut(s)	20
PshAI	GACNNNNGTC	1 cut(s)	1865
PsiI	TTATAA	1 cut(s)	1314
Psp124BI	GAGCTC	5 cut(s)	336, 373, 659, 1015, 1164
Psp1406I	AACGTT	1 cut(s)	754
Psp6I	CCWGG	2 cut(s)	25, 730
PspCI	CACGTG	2 cut(s)	173, 1865
PspGI	CCWGG	2 cut(s)	25, 730
PspN4I	GGNNCC	1 cut(s)	460
PspPI	GGNCC	4 cut(s)	29, 209, 526, 2312
PsrI	GAACNNNNNNTAC	1 cut(s)	30
PsuI	RGATCY	2 cut(s)	108, 934
PvuI	CGATCG	2 cut(s)	1404, 2319
RruI	TCGCGA	1 cut(s)	908
RsaI	GTAC	4 cut(s)	16, 1075, 1280, 1858
RsaNI	GTAC	4 cut(s)	15, 1074, 1279, 1857
RseI	CAYNNNNRTG	2 cut(s)	1250, 1589
SacI	GAGCTC	5 cut(s)	336, 373, 659, 1015, 1164
SatI	GCNGC	6 cut(s)	167, 588, 591, 609, 819, 959
Sau96I	GGNCC	4 cut(s)	29, 209, 526, 2312
SchI	GAGTC	7 cut(s)	63, 274, 294, 1020, 1252, 1611, 2441
ScrFI	CCNGG	5 cut(s)	27, 732, 1287, 1968, 1969
SduI	GDGCHC	5 cut(s)	336, 373, 659, 1015, 1164
SfaNI	GCATC	4 cut(s)	1430, 1455, 1477, 2369
SfcI	CTRYAG	1 cut(s)	2268
Sfr274I	CTCGAG	1 cut(s)	329
SfuI	TTCGAA	1 cut(s)	2411
SinI	GGWCC	3 cut(s)	29, 209, 2312
SlaI	CTCGAG	1 cut(s)	329
SmaI	CCCGGG	1 cut(s)	1969
SmiMI	CAYNNNNRTG	2 cut(s)	1250, 1589
SmlI	CTYRAG	3 cut(s)	329, 350, 973
SmoI	CTYRAG	3 cut(s)	329, 350, 973
Sse9I	AATT	6 cut(s)	901, 1209, 2009, 2140, 2323, 2406
SspI	AATATT	1 cut(s)	201
SspMI	CTAG	7 cut(s)	822, 1082, 1265, 1770, 1875, 2001, 2070
SstI	GAGCTC	5 cut(s)	336, 373, 659, 1015, 1164
StyD4I	CCNGG	5 cut(s)	25, 730, 1285, 1966, 1967
StyI	CCWWGG	1 cut(s)	1006
TaqI	TCGA	7 cut(s)	330, 336, 1602, 2034, 2205, 2351, 2411
TaqII	GACCGA	1 cut(s)	2329
TasI	AATT	6 cut(s)	901, 1209, 2009, 2140, 2323, 2406
TatI	WGTACW	1 cut(s)	1278
TauI	GCSGC	1 cut(s)	961
TfiI	GAWTC	9 cut(s)	70, 393, 616, 847, 1720, 1811, 2147, 2153, 2447
TscAI	CASTG	4 cut(s)	565, 1281, 1638, 2370
TseFI	GTSAC	3 cut(s)	829, 2253, 2419
TseI	GCWGC	5 cut(s)	166, 587, 590, 608, 818
Tsp45I	GTSAC	3 cut(s)	829, 2253, 2419
TspDTI	ATGAA	9 cut(s)	50, 475, 795, 839, 1154, 1240, 1403, 1721, 2022
TspGWI	ACGGA	3 cut(s)	143, 1716, 2348
TspMI	CCCGGG	1 cut(s)	1967
TspRI	CASTG	4 cut(s)	565, 1281, 1638, 2370
Vha464I	CTTAAG	1 cut(s)	350
VpaK11BI	GGWCC	3 cut(s)	29, 209, 2312
XagI	CCTNNNNNAGG	1 cut(s)	894
XapI	RAATTY	4 cut(s)	1209, 2009, 2323, 2406
XceI	RCATGY	1 cut(s)	24
XhoI	CTCGAG	1 cut(s)	329
XmaI	CCCGGG	1 cut(s)	1967
XmiI	GTMKAC	1 cut(s)	1458
XspI	CTAG	7 cut(s)	822, 1082, 1265, 1770, 1875, 2001, 2070
Zsp2I	ATGCAT	1 cut(s)	1470

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.