Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0005582

Physical Location & Seq

Genomic Coordinates Reverse (-)

23245288 .. 23249094

Gene Structure 3807 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1097000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGGCTACAAATACCACAGTTGCAAGTGCATGTCCAACACCGATGACAGCAACATCTAATGGGGTCTTTCAGGGTGATAATCCTCTGGATTTTGCTCTCCCTCTTGCCATCTTACAGATATGCCTTGTGGTTGCACTTACACGGATTCTTGCTTATCTTCTTCGACCACTAAGACAGCCTCGTGTGATTGCCGAGATTGTGGGGGGAATATTACTTGGCCCTTCAGCTCTTGGTCGCAACAAAGACTATCTCGAAGCAATATTTCCAAACAGAAGTCTCACAGTGTTGGATACTTTAGCCAATCTTGGTCTTCTCTTCTTTCTGTTCCTAGTGGGCTTGGAGTTGGATCCTAAGTCCCTCCGCCGCACTGGAAAGAAGGCTCTATGCATTGCACTTGCAGGAATTACCCTACCTTTTGTTTTAGGAATTGGTACATCATTTGCCCTCAAAGGAACTATTTCTAAAGGTGTAGATGGACCACCATTTCTTGTTTTCATGGGAGTGGCTCTTTCTATAACTGCCTTCCCTGTTTTGGCACGTATTTTGGCTGAGCTCAAGCTTTTAACTACTGATATTGGCCGAATGGCCATGTCAGCAGCAGCGGTCAATGATGTGGCTGCGTGGATTCTTCTTGCTCTTGCCATTTCCCTCTCCAGCACTGGCCGTTCTCCCCTGGTTTCCCTATGGGTTCTCTTATGTGGGTGTGCTTTTGTCCTCAGTTGTGTATTTTTTATTCGACCAATCTTTAAATGGATGGCACAGCGCTGTCCCGAGGGTGAACCAGTAGAAGAATTGTATGTATGTGCTACTTTAGTTGCAGTTTTGGCAGCTGGGTTTGTCACTGATACTATTGGAATTCATGCCCTATTTGGAGCATTTGTGCTCGGAATTATTGTCCCAAAGGAAGGGCCATTTGCTGGCGCTCTTGTTGAAAAAGTTGAGGATCTCATATCTGGTCTGTTCCTCCCGTTGTACTTTGTCTCGAGTGGATTGAAGACTGATATAGCTACAATTCGGGGAGCTCAGTCATGGGGCCTCCTCGTTTTGGTCATTTCAACAGCCTGTTTCGGGAAGATCATTGGCACAGTAGCTGTTTCCCTCCTCTGCCGAGTGCCGTTTCAAGAGGCTCTGGCACTCGGGTTCCTCATGAATACTAAAGGGTTAGTGGAGCTTATTGTCCTTAACATCGGTAGAGACAGAAAGGTTTTGAATGATCAAACATTTGCAATCATGGTTCTCATGGCTATCTTTACAACCTTCCTTACGACGCCTGTAGTAATGGCAGTATACAAGCCAGCTAAAAGAAAGAGTATATCTGATTACAAGTACAGAACTATTGAAAGGAAAGATCCAAACACTGAGCTCCGGGTTTTGACCTGTTTCCACGGTACAAGGAACCTCCCCACAATGATTAATCTCATTGAGGCTTCTCGTGGGACTGAAAAGAAGGAACGTCTTTGTGTCTATGCAATGCATCTTATGGAGCTTAATGAGAGATCTTCTGCGATTCTGATGGTTCACAAGGCAAGAAGAAATGGTCTACCGTTTTGGAACAAGGTGAAGGATTCAGATAATCATCAAGTAGTTGTGGCTTTTGAAACGTTTGAGCAGCTGAGTCGAGTGGCTATCCGTCCAATGATAGCAATCTCTTCCGTTTCTAGCATGCATGAGGACATTTGTGCAATGGCTGAAAGGAAAGGGGCAGCAATTATTATTCTCCCATTCCACAAGCACCAGAGGTTGGATGGGGTATTGGAGACAACCCGAACTGAATACAGAGGGGTCAATCGTAGGGTTCTTGAGCATGCTCCATGCTCAGTTGGGATCATGGTGGACCGTGGCCTTGGTGGAAGCACCCATGTATCTGCCAGCAATGTTTCTTCAAGCATAGTTGTCCTATTCTTCGGGGGTAGTGATGATCGTGAGGCCCTTGCTTATGGAATGCGAATGGCTGAGCACCCGGGTAACAGTTTAACAGTTGTCCACTTCTTAGCAAGTCCTGAACTTCAGGGGGAAATAGTCCAAGTGGATATAAACGAGGGCTCCAACACTTCAGCAGGGTCAGCGACTGAGATGTTTCTCGCTGAATTGAAGCAGAAGATTTCAAATAACAGCTCAATCAAATATGAGGAGAGGGCAGTGAGAAATGCTGCAGAAACTACTGATTTGATTCGTGAGTTTAACCGATGCAATCTATTTCTGGTTGGTCGAAGGCCTGATGGTCAAGTAGCTGCTGCCTTGAATCTGAAGGGAGACTGTCCGGAGCTGGGGCCTGTAGGTATCTTGTTAACCTCTCCGGATTTCACAACTACAGCGTCGGTCTTGGTTATGCAGCAGTATCATGGGCTGGAAGTAGTTCCAGGTTCAGTTGGTTTGTCTAAGGTAGTTGTGTTGCCCGAGGACGAAGATTCAGAAACCGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005783 GO:0006605 GO:0006623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0012505 GO:0015031 GO:0015672 GO:0015833 GO:0031410 GO:0031982 GO:0033036 GO:0033365 GO:0034613 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0097708 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

808

Amino Acids

87.32

Weight (kDa)

8.07

Isoelectric Point (pI)

36.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	47 - 425	4.1e-66	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	484 - 625	5.2e-46	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	628 - 782	6.3e-36	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005582)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G23700 AT4G23700
fragaria_vesca	FvH4_5g03731 FvH4_5g03731
malus_domestica	MD00G1094100.v1.1 MD06G1097000.v1.1
prunus_persica	Prupe.5G111700_v2.0.a1 Prupe.5G111700_v2.0.a1 Prupe.5G111700_v2.0.a1 Prupe.5G111700_v2.0.a1 Prupe.5G111700_v2.0.a1 Prupe.5G111700_v2.0.a1
pyrus_communis	pycom06g09310
rosa_chinensis	RchiOBHm_Chr7g0195971
rosa_laevigata	RLG00000004078
rosa_multiflora	Rmu_co8262837.1_g000001 Rmu_sc0004016.1_g000001 Rmu_ssc0000453.1_g000003
rosa_roxburghii	Rroxscaffold_3G00259980
rosa_rugosa	Rorug07G0027700 Rorug07G0027900 Rorug07G0028000 Rorug07G0028100
rosa_samantha	Rh7AG155200 Rh7BG157100 Rh7CG160600 Rh7DG156100
rosa_wichuraiana	Rw7G013270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	917, 1741
AccI	GTMKAC	2 cut(s)	1287, 1540
AccIII	TCCGGA	2 cut(s)	2260, 2296
AciI	CCGC	3 cut(s)	361, 364, 602
AclI	AACGTT	1 cut(s)	1601
AclWI	GGATC	5 cut(s)	341, 354, 951, 1343, 1832
AcoI	YGGCCR	3 cut(s)	577, 585, 661
AcsI	RAATTY	1 cut(s)	855
AcuI	CTGAAG	4 cut(s)	207, 1991, 2037, 2267
AcyI	GRCGYC	1 cut(s)	1268
AfaI	GTAC	4 cut(s)	433, 974, 1328, 1390
AfeI	AGCGCT	1 cut(s)	764
AfiI	CCNNNNNNNGG	7 cut(s)	532, 905, 917, 1045, 1068, 1741, 2267
AjnI	CCWGG	2 cut(s)	672, 2359
Alw21I	GWGCWC	5 cut(s)	555, 885, 1024, 1365, 1959
Alw26I	GTCTC	5 cut(s)	281, 985, 1188, 1751, 2247
AlwI	GGATC	5 cut(s)	341, 354, 951, 1343, 1832
AlwNI	CAGNNNCTG	2 cut(s)	1091, 2069
Ama87I	CYCGRG	5 cut(s)	770, 982, 1136, 1960, 2396
Aor13HI	TCCGGA	2 cut(s)	2260, 2296
Aor51HI	AGCGCT	1 cut(s)	764
ApoI	RAATTY	1 cut(s)	855
AseI	ATTAAT	1 cut(s)	1413
Asp700I	GAANNNNTTC	2 cut(s)	457, 2355
AspLEI	GCGC	2 cut(s)	765, 923
AspS9I	GGNCC	7 cut(s)	218, 476, 908, 1033, 1834, 1927, 2270
AsuC2I	CCSGG	3 cut(s)	1367, 1961, 1962
AsuHPI	GGTGA	3 cut(s)	86, 788, 1570
AvaI	CYCGRG	5 cut(s)	770, 982, 1136, 1960, 2396
AvaII	GGWCC	2 cut(s)	476, 1834
BaeI	ACNNNNGTAYC	2 cut(s)	1845, 1878
BalI	TGGCCA	1 cut(s)	587
BamHI	GGATCC	1 cut(s)	346
BanII	GRGCYC	4 cut(s)	555, 1024, 1365, 2045
BauI	CACGAG	2 cut(s)	180, 1431
BbsI	GAAGAC	2 cut(s)	302, 1001
Bbv12I	GWGCWC	5 cut(s)	555, 885, 1024, 1365, 1959
BccI	CCATC	6 cut(s)	116, 467, 748, 1507, 1739, 2213
BceAI	ACGGC	2 cut(s)	648, 1099
BcgI	CGANNNNNNTGC	2 cut(s)	1598, 1632
BciT130I	CCWGG	2 cut(s)	674, 2361
BciVI	GTATCC	1 cut(s)	283
BclI	TGATCA	1 cut(s)	1213
BcnI	CCSGG	3 cut(s)	1367, 1961, 1962
BcoDI	GTCTC	5 cut(s)	281, 985, 1188, 1751, 2247
BfaI	CTAG	2 cut(s)	329, 1659
BfmI	CTRYAG	4 cut(s)	1272, 2151, 2274, 2310
BfoI	RGCGCY	2 cut(s)	766, 924
BfuI	GTATCC	1 cut(s)	283
BglII	AGATCT	1 cut(s)	1496
BlpI	GCTNAGC	2 cut(s)	549, 1953
Bme1390I	CCNGG	5 cut(s)	674, 1367, 1961, 1962, 2361
Bme18I	GGWCC	2 cut(s)	476, 1834
BmeT110I	CYCGRG	5 cut(s)	770, 982, 1136, 1960, 2396
BmgT120I	GGNCC	7 cut(s)	218, 476, 908, 1033, 1834, 1927, 2270
BmiI	GGNNCC	6 cut(s)	348, 1034, 1142, 1397, 2044, 2271
BmrFI	CCNGG	5 cut(s)	674, 1367, 1961, 1962, 2361
BmsI	GCATC	2 cut(s)	1483, 2177
BpiI	GAAGAC	2 cut(s)	302, 1001
BpmI	CTGGAG	1 cut(s)	637
Bpu1102I	GCTNAGC	2 cut(s)	549, 1953
BpuEI	CTTGAG	2 cut(s)	539, 1820
BpuMI	CCSGG	3 cut(s)	1367, 1961, 1962
BsaAI	YACGTR	1 cut(s)	539
BsaBI	GATNNNNATC	2 cut(s)	1575, 1643
BsaHI	GRCGYC	1 cut(s)	1268
BsaJI	CCNNGG	7 cut(s)	672, 771, 1384, 1837, 1843, 1960, 2397
BsaWI	WCCGGW	2 cut(s)	2260, 2296
BsaXI	ACNNNNNCTCC	4 cut(s)	864, 894, 2027, 2057
Bsc4I	CCNNNNNNNGG	7 cut(s)	532, 905, 917, 1045, 1068, 1741, 2267
Bse118I	RCCGGY	1 cut(s)	2417
Bse1I	ACTGG	3 cut(s)	373, 664, 782
Bse3DI	GCAATG	4 cut(s)	387, 1476, 1689, 1879
Bse8I	GATNNNNATC	2 cut(s)	1575, 1643
BseAI	TCCGGA	2 cut(s)	2260, 2296
BseBI	CCWGG	2 cut(s)	674, 2361
BseDI	CCNNGG	7 cut(s)	672, 771, 1384, 1837, 1843, 1960, 2397
BseGI	GGATG	2 cut(s)	759, 1750
BseJI	GATNNNNATC	2 cut(s)	1575, 1643
BseLI	CCNNNNNNNGG	7 cut(s)	532, 905, 917, 1045, 1068, 1741, 2267
BseMI	GCAATG	4 cut(s)	387, 1476, 1689, 1879
BseMII	CTCAG	8 cut(s)	540, 730, 1037, 1350, 1604, 1830, 1944, 2061
BseNI	ACTGG	3 cut(s)	373, 664, 782
BseRI	GAGGAG	3 cut(s)	1028, 1091, 2144
BseYI	CCCAGC	2 cut(s)	830, 2266
BsiHKAI	GWGCWC	5 cut(s)	555, 885, 1024, 1365, 1959
BsiHKCI	CYCGRG	5 cut(s)	770, 982, 1136, 1960, 2396
BsiSI	CCGG	5 cut(s)	1366, 1961, 2261, 2297, 2418
BslFI	GGGAC	4 cut(s)	340, 753, 881, 1450
BslI	CCNNNNNNNGG	7 cut(s)	532, 905, 917, 1045, 1068, 1741, 2267
BsmAI	GTCTC	5 cut(s)	281, 985, 1188, 1751, 2247
BsmFI	GGGAC	4 cut(s)	340, 753, 881, 1450
BsmI	GAATGC	1 cut(s)	1947
BsoBI	CYCGRG	5 cut(s)	770, 982, 1136, 1960, 2396
Bsp1286I	GDGCHC	6 cut(s)	555, 885, 1024, 1365, 1959, 2045
Bsp13I	TCCGGA	2 cut(s)	2260, 2296
Bsp143I	GATC	8 cut(s)	346, 943, 1074, 1213, 1348, 1496, 1824, 1918
Bsp1720I	GCTNAGC	2 cut(s)	549, 1953
BspACI	CCGC	3 cut(s)	361, 364, 602
BspCNI	CTCAG	8 cut(s)	541, 729, 1036, 1351, 1605, 1829, 1945, 2062
BspEI	TCCGGA	2 cut(s)	2260, 2296
BspHI	TCATGA	1 cut(s)	1146
BspLI	GGNNCC	6 cut(s)	348, 1034, 1142, 1397, 2044, 2271
BspMAI	CTGCAG	1 cut(s)	2155
BspPI	GGATC	5 cut(s)	341, 354, 951, 1343, 1832
BsrDI	GCAATG	4 cut(s)	387, 1476, 1689, 1879
BsrFI	RCCGGY	1 cut(s)	2417
BsrI	ACTGG	3 cut(s)	373, 664, 782
BssAI	RCCGGY	1 cut(s)	2417
BssECI	CCNNGG	7 cut(s)	672, 771, 1384, 1837, 1843, 1960, 2397
BssMI	GATC	8 cut(s)	346, 943, 1074, 1213, 1348, 1496, 1824, 1918
BssNAI	GTATAC	1 cut(s)	1288
BssNI	GRCGYC	1 cut(s)	1268
BssSI	CACGAG	2 cut(s)	180, 1431
BssT1I	CCWWGG	1 cut(s)	1843
Bst1107I	GTATAC	1 cut(s)	1288
Bst2BI	CACGAG	2 cut(s)	180, 1431
Bst2UI	CCWGG	2 cut(s)	674, 2361
Bst4CI	ACNGT	9 cut(s)	19, 283, 1087, 1388, 1545, 1838, 1970, 1978, 2258
Bst6I	CTCTTC	2 cut(s)	320, 1654
BstACI	GRCGYC	1 cut(s)	1268
BstBAI	YACGTR	1 cut(s)	539
BstC8I	GCNNGC	5 cut(s)	919, 1296, 1664, 1806, 1870
BstDSI	CCRYGG	2 cut(s)	1384, 1837
BstF5I	GGATG	2 cut(s)	759, 1750
BstH2I	RGCGCY	2 cut(s)	766, 924
BstHHI	GCGC	2 cut(s)	765, 923
BstKTI	GATC	8 cut(s)	349, 946, 1077, 1216, 1351, 1499, 1827, 1921
BstMAI	GTCTC	5 cut(s)	281, 985, 1188, 1751, 2247
BstMBI	GATC	8 cut(s)	346, 943, 1074, 1213, 1348, 1496, 1824, 1918
BstMWI	GCNNNNNNNGC	3 cut(s)	593, 824, 2063
BstNI	CCWGG	2 cut(s)	674, 2361
BstNSI	RCATGY	3 cut(s)	33, 1666, 1808
BstSCI	CCNGG	5 cut(s)	672, 1365, 1959, 1960, 2359
BstSFI	CTRYAG	4 cut(s)	1272, 2151, 2274, 2310
BstV2I	GAAGAC	2 cut(s)	302, 1001
BstX2I	RGATCY	4 cut(s)	346, 943, 1348, 1496
BstYI	RGATCY	4 cut(s)	346, 943, 1348, 1496
BstZ17I	GTATAC	1 cut(s)	1288
BsuI	GTATCC	1 cut(s)	283
BtgI	CCRYGG	2 cut(s)	1384, 1837
BtsCI	GGATG	2 cut(s)	759, 1750
BtsI	GCAGTG	1 cut(s)	2145
BtsIMutI	CAGTG	6 cut(s)	288, 366, 657, 840, 1356, 2145
Cac8I	GCNNGC	5 cut(s)	919, 1296, 1664, 1806, 1870
CaiI	CAGNNNCTG	2 cut(s)	1091, 2069
CciI	TCATGA	1 cut(s)	1146
CfoI	GCGC	2 cut(s)	765, 923
Cfr10I	RCCGGY	1 cut(s)	2417
Cfr13I	GGNCC	7 cut(s)	218, 476, 908, 1033, 1834, 1927, 2270
Cfr9I	CCCGGG	1 cut(s)	1960
CseI	GACGC	2 cut(s)	1276, 2304
Csp6I	GTAC	4 cut(s)	432, 973, 1327, 1389
CviQI	GTAC	4 cut(s)	432, 973, 1327, 1389
DpnI	GATC	8 cut(s)	348, 945, 1076, 1215, 1350, 1498, 1826, 1920
DpnII	GATC	8 cut(s)	346, 943, 1074, 1213, 1348, 1496, 1824, 1918
DraI	TTTAAA	1 cut(s)	748
EaeI	YGGCCR	3 cut(s)	577, 585, 661
Eam1104I	CTCTTC	2 cut(s)	320, 1654
EarI	CTCTTC	2 cut(s)	320, 1654
EciI	GGCGGA	1 cut(s)	350
Ecl136II	GAGCTC	3 cut(s)	553, 1022, 1363
Eco130I	CCWWGG	1 cut(s)	1843
Eco147I	AGGCCT	1 cut(s)	2215
Eco24I	GRGCYC	4 cut(s)	555, 1024, 1365, 2045
Eco47I	GGWCC	2 cut(s)	476, 1834
Eco47III	AGCGCT	1 cut(s)	764
Eco53kI	GAGCTC	3 cut(s)	553, 1022, 1363
Eco57I	CTGAAG	4 cut(s)	207, 1991, 2037, 2267
Eco88I	CYCGRG	5 cut(s)	770, 982, 1136, 1960, 2396
EcoICRI	GAGCTC	3 cut(s)	553, 1022, 1363
EcoO109I	RGGNCCY	3 cut(s)	1033, 1927, 2270
EcoRI	GAATTC	1 cut(s)	855
EcoRII	CCWGG	2 cut(s)	672, 2359
EcoT14I	CCWWGG	1 cut(s)	1843
EcoT22I	ATGCAT	3 cut(s)	389, 1476, 1668
EcoT38I	GRGCYC	4 cut(s)	555, 1024, 1365, 2045
ErhI	CCWWGG	1 cut(s)	1843
FaqI	GGGAC	4 cut(s)	340, 753, 881, 1450
FbaI	TGATCA	1 cut(s)	1213
FblI	GTMKAC	2 cut(s)	1287, 1540
FokI	GGATG	2 cut(s)	766, 1757
FriOI	GRGCYC	4 cut(s)	555, 1024, 1365, 2045
FspBI	CTAG	2 cut(s)	329, 1659
GlaI	GCGC	2 cut(s)	764, 922
GsaI	CCCAGC	2 cut(s)	834, 2270
GsuI	CTGGAG	1 cut(s)	637
HaeII	RGCGCY	2 cut(s)	766, 924
HapII	CCGG	5 cut(s)	1366, 1961, 2261, 2297, 2418
HgaI	GACGC	2 cut(s)	1276, 2304
HhaI	GCGC	2 cut(s)	765, 923
Hin1I	GRCGYC	1 cut(s)	1268
Hin6I	GCGC	2 cut(s)	763, 921
HinP1I	GCGC	2 cut(s)	763, 921
HincII	GTYRAC	1 cut(s)	2289
HindII	GTYRAC	1 cut(s)	2289
HindIII	AAGCTT	1 cut(s)	557
HinfI	GANTC	8 cut(s)	145, 625, 1507, 1565, 1615, 2170, 2242, 2408
HpaI	GTTAAC	1 cut(s)	2289
HpaII	CCGG	5 cut(s)	1366, 1961, 2261, 2297, 2418
HphI	GGTGA	3 cut(s)	86, 788, 1570
Hpy166II	GTNNAC	7 cut(s)	779, 1288, 1519, 1541, 1834, 1984, 2289
Hpy188I	TCNGA	6 cut(s)	887, 1318, 1512, 1570, 2247, 2413
Hpy8I	GTNNAC	7 cut(s)	779, 1288, 1519, 1541, 1834, 1984, 2289
Hpy99I	CGWCG	2 cut(s)	1270, 2320
HpyAV	CCTTC	9 cut(s)	231, 370, 532, 899, 1267, 1441, 1555, 2205, 2242
HpyCH4III	ACNGT	9 cut(s)	19, 283, 1087, 1388, 1545, 1838, 1970, 1978, 2258
HpyCH4IV	ACGT	3 cut(s)	538, 1453, 1601
HpyF10VI	GCNNNNNNNGC	3 cut(s)	593, 824, 2063
HpySE526I	ACGT	3 cut(s)	538, 1453, 1601
Hsp92I	GRCGYC	1 cut(s)	1268
HspAI	GCGC	2 cut(s)	763, 921
Kpn2I	TCCGGA	2 cut(s)	2260, 2296
Ksp22I	TGATCA	1 cut(s)	1213
KspAI	GTTAAC	1 cut(s)	2289
Kzo9I	GATC	8 cut(s)	346, 943, 1074, 1213, 1348, 1496, 1824, 1918
LmnI	GCTCC	8 cut(s)	872, 1019, 1168, 1368, 1483, 1813, 2048, 2263
LweI	GCATC	2 cut(s)	1483, 2177
MaeI	CTAG	2 cut(s)	329, 1659
MaeII	ACGT	3 cut(s)	538, 1453, 1601
MaeIII	GTNAC	2 cut(s)	838, 1964
MalI	GATC	8 cut(s)	348, 945, 1076, 1215, 1350, 1498, 1826, 1920
MboI	GATC	8 cut(s)	346, 943, 1074, 1213, 1348, 1496, 1824, 1918
MflI	RGATCY	4 cut(s)	346, 943, 1348, 1496
MhlI	GDGCHC	6 cut(s)	555, 885, 1024, 1365, 1959, 2045
MlsI	TGGCCA	1 cut(s)	587
MluCI	AATT	8 cut(s)	402, 426, 791, 855, 888, 1011, 1707, 2087
MluNI	TGGCCA	1 cut(s)	587
MlyI	GAGTC	1 cut(s)	1624
MmeI	TCCRAC	5 cut(s)	59, 267, 324, 1722, 2070
Mox20I	TGGCCA	1 cut(s)	587
Mph1103I	ATGCAT	3 cut(s)	389, 1476, 1668
MroI	TCCGGA	2 cut(s)	2260, 2296
MroXI	GAANNNNTTC	2 cut(s)	457, 2355
MscI	TGGCCA	1 cut(s)	587
MseI	TTAA	8 cut(s)	563, 747, 1182, 1413, 1488, 1973, 2181, 2288
MslI	CAYNNNNRTG	1 cut(s)	500
Msp20I	TGGCCA	1 cut(s)	587
MspA1I	CMGCKG	3 cut(s)	602, 830, 1612
MspI	CCGG	5 cut(s)	1366, 1961, 2261, 2297, 2418
MspR9I	CCNGG	5 cut(s)	674, 1367, 1961, 1962, 2361
Mva1269I	GAATGC	1 cut(s)	1947
MvaI	CCWGG	2 cut(s)	674, 2361
MwoI	GCNNNNNNNGC	3 cut(s)	593, 824, 2063
NciI	CCSGG	3 cut(s)	1367, 1961, 1962
NdeII	GATC	8 cut(s)	346, 943, 1074, 1213, 1348, 1496, 1824, 1918
NlaIV	GGNNCC	6 cut(s)	348, 1034, 1142, 1397, 2044, 2271
NmeAIII	GCCGAG	2 cut(s)	217, 1133
NmuCI	GTSAC	1 cut(s)	838
NsiI	ATGCAT	3 cut(s)	389, 1476, 1668
NspI	RCATGY	3 cut(s)	33, 1666, 1808
PaeI	GCATGC	2 cut(s)	1666, 1808
PaeR7I	CTCGAG	1 cut(s)	982
PagI	TCATGA	1 cut(s)	1146
PceI	AGGCCT	1 cut(s)	2215
PctI	GAATGC	1 cut(s)	1947
PdmI	GAANNNNTTC	2 cut(s)	457, 2355
PfeI	GAWTC	7 cut(s)	145, 625, 1507, 1565, 2170, 2242, 2408
PflMI	CCANNNNNTGG	2 cut(s)	917, 1741
PleI	GAGTC	1 cut(s)	1623
PpsI	GAGTC	1 cut(s)	1623
Ppu21I	YACGTR	1 cut(s)	539
PshBI	ATTAAT	1 cut(s)	1413
Psp124BI	GAGCTC	3 cut(s)	555, 1024, 1365
Psp1406I	AACGTT	1 cut(s)	1601
Psp6I	CCWGG	2 cut(s)	672, 2359
PspFI	CCCAGC	2 cut(s)	830, 2266
PspGI	CCWGG	2 cut(s)	672, 2359
PspN4I	GGNNCC	6 cut(s)	348, 1034, 1142, 1397, 2044, 2271
PspPI	GGNCC	7 cut(s)	218, 476, 908, 1033, 1834, 1927, 2270
PstI	CTGCAG	1 cut(s)	2155
PstNI	CAGNNNCTG	2 cut(s)	1091, 2069
PsuI	RGATCY	4 cut(s)	346, 943, 1348, 1496
PvuII	CAGCTG	2 cut(s)	830, 1612
RsaI	GTAC	4 cut(s)	433, 974, 1328, 1390
RsaNI	GTAC	4 cut(s)	432, 973, 1327, 1389
RseI	CAYNNNNRTG	1 cut(s)	500
SacI	GAGCTC	3 cut(s)	555, 1024, 1365
SaqAI	TTAA	8 cut(s)	563, 747, 1182, 1413, 1488, 1973, 2181, 2288
Sau3AI	GATC	8 cut(s)	346, 943, 1074, 1213, 1348, 1496, 1824, 1918
Sau96I	GGNCC	7 cut(s)	218, 476, 908, 1033, 1834, 1927, 2270
SchI	GAGTC	1 cut(s)	1624
ScrFI	CCNGG	5 cut(s)	674, 1367, 1961, 1962, 2361
SduI	GDGCHC	6 cut(s)	555, 885, 1024, 1365, 1959, 2045
SfaNI	GCATC	2 cut(s)	1483, 2177
SfcI	CTRYAG	4 cut(s)	1272, 2151, 2274, 2310
Sfr274I	CTCGAG	1 cut(s)	982
SinI	GGWCC	2 cut(s)	476, 1834
SlaI	CTCGAG	1 cut(s)	982
SmaI	CCCGGG	1 cut(s)	1962
SmiMI	CAYNNNNRTG	1 cut(s)	500
SmlI	CTYRAG	3 cut(s)	554, 982, 1799
SmoI	CTYRAG	3 cut(s)	554, 982, 1799
SphI	GCATGC	2 cut(s)	1666, 1808
Sse9I	AATT	8 cut(s)	402, 426, 791, 855, 888, 1011, 1707, 2087
SseBI	AGGCCT	1 cut(s)	2215
SsiI	CCGC	3 cut(s)	361, 364, 602
SspI	AATATT	2 cut(s)	210, 261
SspMI	CTAG	2 cut(s)	329, 1659
SstI	GAGCTC	3 cut(s)	555, 1024, 1365
StuI	AGGCCT	1 cut(s)	2215
StyD4I	CCNGG	5 cut(s)	672, 1365, 1959, 1960, 2359
StyI	CCWWGG	1 cut(s)	1843
TaaI	ACNGT	9 cut(s)	19, 283, 1087, 1388, 1545, 1838, 1970, 1978, 2258
TaiI	ACGT	3 cut(s)	541, 1456, 1604
TaqI	TCGA	6 cut(s)	163, 252, 736, 983, 1618, 2209
TaqII	GACCGA	1 cut(s)	2308
TasI	AATT	8 cut(s)	402, 426, 791, 855, 888, 1011, 1707, 2087
TatI	WGTACW	2 cut(s)	972, 1326
TauI	GCSGC	1 cut(s)	366
TfiI	GAWTC	7 cut(s)	145, 625, 1507, 1565, 2170, 2242, 2408
Tru1I	TTAA	8 cut(s)	563, 747, 1182, 1413, 1488, 1973, 2181, 2288
Tru9I	TTAA	8 cut(s)	563, 747, 1182, 1413, 1488, 1973, 2181, 2288
TscAI	CASTG	6 cut(s)	288, 373, 664, 847, 1363, 2145
TseFI	GTSAC	1 cut(s)	838
Tsp45I	GTSAC	1 cut(s)	838
TspDTI	ATGAA	3 cut(s)	484, 848, 1163
TspGWI	ACGGA	3 cut(s)	157, 1619, 1642
TspMI	CCCGGG	1 cut(s)	1960
TspRI	CASTG	6 cut(s)	288, 373, 664, 847, 1363, 2145
Van91I	CCANNNNNTGG	2 cut(s)	917, 1741
VpaK11BI	GGWCC	2 cut(s)	476, 1834
VspI	ATTAAT	1 cut(s)	1413
XapI	RAATTY	1 cut(s)	855
XceI	RCATGY	3 cut(s)	33, 1666, 1808
XcmI	CCANNNNNNNNNTGG	2 cut(s)	747, 1742
XhoI	CTCGAG	1 cut(s)	982
XmaI	CCCGGG	1 cut(s)	1960
XmiI	GTMKAC	2 cut(s)	1287, 1540
XmnI	GAANNNNTTC	2 cut(s)	457, 2355
XspI	CTAG	2 cut(s)	329, 1659
Zsp2I	ATGCAT	3 cut(s)	389, 1476, 1668

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.