Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0005582

Physical Location & Seq

Genomic Coordinates Reverse (-)

19673919 .. 19679379

Gene Structure 5461 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1094100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2421 bp

ATGGCTACAAATACCACAGTTGCAACTGCATGTCCAGCACCGATGAAAGCCACATCTAATGGGGTCTTTCAGGGTGATAATCCTCTGGATTATGCTCTCCCTCTTGCCATCTTACAGATATGCCTTGTGGTTACACTTACACGGATTCTTGCTTATCTTCTTCGACCACTAAGACAGCCTCGTGTGATTGCAGAGATTGTGGGTGGAATATTACTTGGCCCTTCAGCTCTTGGTCACAACAAAGACTATATCGAAGCAATATTTCCAAAAAGAAGTCTCACAGTGTTGGATACTTTAGCCAATCTTGGTCTTCTCTTCTTTTTGTTCATAGTGGGATTGGAGTTGGATCCTAAGTCCATCCGCCGCACTGGAAAGAAGGCTCTCTGCATTGCACTTGCAGGAATTATCTTACCTTTTGTTTTAGGAATTGGTACATCGTTTGCCCTCAAAGAAACTATTTCTAAAGGTGTAGATGGACCACCATTTCTTGTTTTCATGGGAGTGGCTCTTTCTATAACTGCCTTCCCTGTTCTGGCTCGTATTTTGGCTGAGCTCAAGCTTTTAACCACTGATATTGGCCGAATGGCCATGTCAGCAGCCGCGATCAATGATGTGGCTGCGTGGATTCTTCTTGCTCTTGCCATTTCCCTCTCTGGCACTGGCCGTTCTCCCCTGGTTTCGCTATGGGTATTGTTGTGTGGGTGTGCTTTTGTCCTCGGTTGTGTATTTTTCGTTCGACCAATCTTTAAATGGATGGCACAGCGCTGTCCCGAAGGTGAACCAGTAGAAGAATTGTATGTATGTGCTACTTTAGTTGCAGTTTTGGCAGCTGGGTTTGTCACTGATACTATTGGAATTCATGCCCTATTTGGAGCTTTTGTGCTCGGAATCATTGTCCCAAAGGAAGGGCCATTTGCAGGCGCTCTTGTTGAAAAAGTTGAGGATCTTGTATCTGGTCTATTCCTCCCATTGTACTTTGTCTCTAGTGGATTGAAGACTGATATAGCTACAATTCATGGAGCTCAGTCATGGGGCCTCCTCGTTTTGGTCATTTCAACAGCCTGTTTCGGAAAGGTCTTTGGCACGGTAGCTGTTTCCCTCCTCTGCCGGCTGCCCTTTCAAGAGGCTCTGGCACTCGGGTTCCTCATGAATACTAAAGGGTTGGTGGAGCTCATTGTCCTTAACATCGGTAGAGAGAGAAAGGTTTTGAATGATCAAACGTTTGCTATCATGGTTCTCATGGCTATCTTCACAACCTTCATTACGACACCTGTAGTAATGGCAGTATACAAGCCAGCTAAAAGAAAGAGTACATCTGATTATAAGTACAGAACAATTGAAAGGAAAGATCCAAACACTGAGCTCCAGATTTTGACCTGTTTCCACGGTACAAGGAACCTCCCCACAATGATCAATCTCATCGAGGCTTCTCGTGGGACTGGAAAGAAGGAACGACTTTGTGTCTATGCAATGCATCTTATGGAGCTTAATGAGAGATCTTCTGCGATTCTGATGGTTCACAAGGCGAGAAGAAATGGGCTACCCTTTTGGAACAAGGTGACGGATTCAGGTAATAATCAATTAGTTGTGGCTTTTGAAACGTTTGAGCAGCTGAGTCGAGTGGCTATCCGTCCAATGACAGCAATCTCTTCCGTTTCTAGCATGCATGAGGACATCTGTGCAATGGCTGAAAGGAAAAGGGCAGCAATTATTATTGTCCCATTCCACAAGCACCAGAGGTTGGATGGGGTATTGGTGACAACTCGAACTGAATACAGAGGGGTCAACCAAAGGGTTCTTGAGCATGCGCCATGTTCAGTGGGGATCATGGTGGACCGTGGCCTTGGTGGAAGCACCCATGTATCTGCCAGCAATGTTTCTTCAAGCATAGTTGTCGTATTCTTCGGGGGTAGTGATGATCATGAGGCCCTTGCTTATGGGATGCGAATGGCTGAGCACCCGGGTAACAATTTAACCGTCGTCCACTTCTTAGCAAGTCCTGAACTTCAGCGGGAGATAGTCCAAGTGGACATAAACGAGGGCTTCAACACTTCAGCAGGGTCAGTGAACGAGAAGTTCATTGCTGAATTGAAGAAGGTTCCAAATGACAGCTCAATCAAATATGAGGAGAGGGTAGTGAGAAATGCTGCAGAAACTACTGATTTGATTCGTGAGTTTAACCGATGCAATCTGTTTCTGGTTGGTAGAAGGCCCGAAGGTCAAGTAGCTGCTGCCTTGAATATCAAGGGAGACTGTCCGGAGCTGGGGCCCGTAGGTAGCCTGTTGACCTCTCCAGATTTCACAACTACAGCTTCGGTCTTGGTTGTACAGCAGTATCTTGGGATGGCAGTAGTTCCAGGTTCAGTTGGTCTGTCGAAGGTCGTCGTGTTGCCTGAGGACGAAGATTCAGAAACCGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005783 GO:0006605 GO:0006623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0012505 GO:0015031 GO:0015672 GO:0015833 GO:0031410 GO:0031982 GO:0033036 GO:0033365 GO:0034613 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0097708 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

807

Amino Acids

87.22

Weight (kDa)

8.24

Isoelectric Point (pI)

37.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	48 - 425	1.7e-65	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	484 - 625	1.1e-47	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	628 - 781	1e-37	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005582)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G23700 AT4G23700
fragaria_vesca	FvH4_5g03731 FvH4_5g03731
malus_domestica	MD00G1094100.v1.1 MD06G1097000.v1.1
prunus_persica	Prupe.5G111700_v2.0.a1 Prupe.5G111700_v2.0.a1 Prupe.5G111700_v2.0.a1 Prupe.5G111700_v2.0.a1 Prupe.5G111700_v2.0.a1 Prupe.5G111700_v2.0.a1
pyrus_communis	pycom06g09310
rosa_chinensis	RchiOBHm_Chr7g0195971
rosa_laevigata	RLG00000004078
rosa_multiflora	Rmu_co8262837.1_g000001 Rmu_sc0004016.1_g000001 Rmu_ssc0000453.1_g000003
rosa_roxburghii	Rroxscaffold_3G00259980
rosa_rugosa	Rorug07G0027700 Rorug07G0027900 Rorug07G0028000 Rorug07G0028100
rosa_samantha	Rh7AG155200 Rh7BG157100 Rh7CG160600 Rh7DG156100
rosa_wichuraiana	Rw7G013270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1323
AccB7I	CCANNNNNTGG	1 cut(s)	1741
AccI	GTMKAC	1 cut(s)	1287
AccII	CGCG	1 cut(s)	602
AccIII	TCCGGA	1 cut(s)	2257
AciI	CCGC	4 cut(s)	361, 364, 600, 2011
AclI	AACGTT	2 cut(s)	1220, 1601
AclWI	GGATC	5 cut(s)	341, 354, 951, 1343, 1832
AcoI	YGGCCR	3 cut(s)	577, 585, 661
AcsI	RAATTY	1 cut(s)	855
AcuI	CTGAAG	3 cut(s)	207, 1991, 2037
AfaI	GTAC	6 cut(s)	433, 974, 1312, 1328, 1390, 2328
AfeI	AGCGCT	1 cut(s)	764
AfiI	CCNNNNNNNGG	6 cut(s)	532, 905, 917, 1045, 1741, 2264
AhdI	GACNNNNNGTC	1 cut(s)	1460
AjnI	CCWGG	2 cut(s)	672, 2356
Alw21I	GWGCWC	6 cut(s)	555, 885, 1024, 1173, 1365, 1959
Alw26I	GTCTC	3 cut(s)	281, 985, 2244
AlwI	GGATC	5 cut(s)	341, 354, 951, 1343, 1832
Ama87I	CYCGRG	2 cut(s)	1136, 1960
Aor13HI	TCCGGA	1 cut(s)	2257
Aor51HI	AGCGCT	1 cut(s)	764
ApaI	GGGCCC	1 cut(s)	2271
ApeKI	GCWGC	9 cut(s)	596, 617, 827, 1111, 1609, 1703, 2147, 2228, 2231
ApoI	RAATTY	1 cut(s)	855
AspLEI	GCGC	3 cut(s)	765, 923, 1810
AspS9I	GGNCC	9 cut(s)	218, 476, 908, 1033, 1834, 1927, 2211, 2267, 2268
AsuC2I	CCSGG	2 cut(s)	1961, 1962
AsuHPI	GGTGA	4 cut(s)	86, 788, 1570, 1768
AvaI	CYCGRG	2 cut(s)	1136, 1960
AvaII	GGWCC	2 cut(s)	476, 1834
AxyI	CCTNAGG	1 cut(s)	2394
BaeGI	GKGCMC	1 cut(s)	2271
BaeI	ACNNNNGTAYC	4 cut(s)	1845, 1878, 2318, 2351
BalI	TGGCCA	1 cut(s)	587
BamHI	GGATCC	1 cut(s)	346
BanII	GRGCYC	5 cut(s)	555, 1024, 1173, 1365, 2271
BauI	CACGAG	2 cut(s)	180, 1431
BbsI	GAAGAC	2 cut(s)	302, 1001
Bbv12I	GWGCWC	6 cut(s)	555, 885, 1024, 1173, 1365, 1959
BbvI	GCAGC	9 cut(s)	604, 608, 839, 1098, 1621, 1715, 2134, 2215, 2218
BccI	CCATC	7 cut(s)	116, 365, 467, 748, 1507, 1739, 2338
BceAI	ACGGC	1 cut(s)	648
BcgI	CGANNNNNNTGC	4 cut(s)	1598, 1632, 1876, 1910
BciT130I	CCWGG	2 cut(s)	674, 2358
BciVI	GTATCC	1 cut(s)	283
BclI	TGATCA	3 cut(s)	1213, 1410, 1918
BcnI	CCSGG	2 cut(s)	1961, 1962
BcoDI	GTCTC	3 cut(s)	281, 985, 2244
BfaI	CTAG	2 cut(s)	984, 1659
BfmI	CTRYAG	3 cut(s)	1272, 2148, 2307
BfoI	RGCGCY	2 cut(s)	766, 924
BfuI	GTATCC	1 cut(s)	283
BglII	AGATCT	1 cut(s)	1496
BlpI	GCTNAGC	2 cut(s)	549, 1953
Bme1390I	CCNGG	4 cut(s)	674, 1961, 1962, 2358
Bme18I	GGWCC	2 cut(s)	476, 1834
BmeRI	GACNNNNNGTC	1 cut(s)	1460
BmeT110I	CYCGRG	2 cut(s)	1136, 1960
BmgT120I	GGNCC	9 cut(s)	218, 476, 908, 1033, 1834, 1927, 2211, 2267, 2268
BmiI	GGNNCC	7 cut(s)	348, 1034, 1142, 1397, 2100, 2268, 2269
BmrFI	CCNGG	4 cut(s)	674, 1961, 1962, 2358
BmsI	GCATC	3 cut(s)	1483, 1932, 2174
BpiI	GAAGAC	2 cut(s)	302, 1001
BpmI	CTGGAG	2 cut(s)	1349, 2277
Bpu1102I	GCTNAGC	2 cut(s)	549, 1953
BpuEI	CTTGAG	2 cut(s)	539, 1820
BpuMI	CCSGG	2 cut(s)	1961, 1962
BsaJI	CCNNGG	6 cut(s)	672, 715, 1384, 1837, 1843, 1960
BsaWI	WCCGGW	1 cut(s)	2257
BsaXI	ACNNNNNCTCC	2 cut(s)	864, 894
Bsc4I	CCNNNNNNNGG	6 cut(s)	532, 905, 917, 1045, 1741, 2264
Bse118I	RCCGGY	2 cut(s)	1107, 2414
Bse1I	ACTGG	4 cut(s)	373, 664, 782, 1444
Bse21I	CCTNAGG	1 cut(s)	2394
Bse3DI	GCAATG	5 cut(s)	387, 1476, 1689, 1879, 2079
BseAI	TCCGGA	1 cut(s)	2257
BseBI	CCWGG	2 cut(s)	674, 2358
BseDI	CCNNGG	6 cut(s)	672, 715, 1384, 1837, 1843, 1960
BseGI	GGATG	5 cut(s)	357, 759, 1750, 1947, 2349
BseLI	CCNNNNNNNGG	6 cut(s)	532, 905, 917, 1045, 1741, 2264
BseMI	GCAATG	5 cut(s)	387, 1476, 1689, 1879, 2079
BseMII	CTCAG	6 cut(s)	540, 1037, 1350, 1604, 1944, 2385
BseNI	ACTGG	4 cut(s)	373, 664, 782, 1444
BseRI	GAGGAG	3 cut(s)	1028, 1091, 2141
BseSI	GKGCMC	1 cut(s)	2271
BseXI	GCAGC	9 cut(s)	604, 608, 839, 1098, 1621, 1715, 2134, 2215, 2218
BseYI	CCCAGC	2 cut(s)	830, 2263
Bsh1236I	CGCG	1 cut(s)	602
BsiHKAI	GWGCWC	6 cut(s)	555, 885, 1024, 1173, 1365, 1959
BsiHKCI	CYCGRG	2 cut(s)	1136, 1960
BsiSI	CCGG	4 cut(s)	1108, 1961, 2258, 2415
BslFI	GGGAC	4 cut(s)	753, 881, 1450, 1703
BslI	CCNNNNNNNGG	6 cut(s)	532, 905, 917, 1045, 1741, 2264
BsmAI	GTCTC	3 cut(s)	281, 985, 2244
BsmFI	GGGAC	4 cut(s)	753, 881, 1450, 1703
BsoBI	CYCGRG	2 cut(s)	1136, 1960
Bsp120I	GGGCCC	1 cut(s)	2267
Bsp1286I	GDGCHC	7 cut(s)	555, 885, 1024, 1173, 1365, 1959, 2271
Bsp13I	TCCGGA	1 cut(s)	2257
Bsp1407I	TGTACA	1 cut(s)	2326
Bsp143I	GATC	9 cut(s)	346, 603, 943, 1213, 1348, 1410, 1496, 1824, 1918
Bsp1720I	GCTNAGC	2 cut(s)	549, 1953
BspACI	CCGC	4 cut(s)	361, 364, 600, 2011
BspCNI	CTCAG	6 cut(s)	541, 1036, 1351, 1605, 1945, 2386
BspEI	TCCGGA	1 cut(s)	2257
BspFNI	CGCG	1 cut(s)	602
BspHI	TCATGA	2 cut(s)	1146, 1921
BspLI	GGNNCC	7 cut(s)	348, 1034, 1142, 1397, 2100, 2268, 2269
BspMAI	CTGCAG	1 cut(s)	2152
BspPI	GGATC	5 cut(s)	341, 354, 951, 1343, 1832
BsrDI	GCAATG	5 cut(s)	387, 1476, 1689, 1879, 2079
BsrFI	RCCGGY	2 cut(s)	1107, 2414
BsrGI	TGTACA	1 cut(s)	2326
BsrI	ACTGG	4 cut(s)	373, 664, 782, 1444
BssAI	RCCGGY	2 cut(s)	1107, 2414
BssECI	CCNNGG	6 cut(s)	672, 715, 1384, 1837, 1843, 1960
BssMI	GATC	9 cut(s)	346, 603, 943, 1213, 1348, 1410, 1496, 1824, 1918
BssNAI	GTATAC	1 cut(s)	1288
BssSI	CACGAG	2 cut(s)	180, 1431
BssT1I	CCWWGG	1 cut(s)	1843
Bst1107I	GTATAC	1 cut(s)	1288
Bst2BI	CACGAG	2 cut(s)	180, 1431
Bst2UI	CCWGG	2 cut(s)	674, 2358
Bst4CI	ACNGT	7 cut(s)	19, 283, 1087, 1388, 1838, 1978, 2255
Bst6I	CTCTTC	2 cut(s)	320, 1654
BstAUI	TGTACA	1 cut(s)	2326
BstC8I	GCNNGC	6 cut(s)	919, 1109, 1296, 1664, 1806, 1870
BstDEI	CTNAG	9 cut(s)	170, 351, 549, 1023, 1359, 1613, 1953, 1990, 2394
BstDSI	CCRYGG	2 cut(s)	1384, 1837
BstF5I	GGATG	5 cut(s)	357, 759, 1750, 1947, 2349
BstFNI	CGCG	1 cut(s)	602
BstH2I	RGCGCY	2 cut(s)	766, 924
BstHHI	GCGC	3 cut(s)	765, 923, 1810
BstKTI	GATC	9 cut(s)	349, 606, 946, 1216, 1351, 1413, 1499, 1827, 1921
BstMAI	GTCTC	3 cut(s)	281, 985, 2244
BstMBI	GATC	9 cut(s)	346, 603, 943, 1213, 1348, 1410, 1496, 1824, 1918
BstMWI	GCNNNNNNNGC	3 cut(s)	35, 593, 824
BstNI	CCWGG	2 cut(s)	674, 2358
BstNSI	RCATGY	3 cut(s)	33, 1666, 1808
BstSCI	CCNGG	4 cut(s)	672, 1959, 1960, 2356
BstSFI	CTRYAG	3 cut(s)	1272, 2148, 2307
BstSLI	GKGCMC	1 cut(s)	2271
BstUI	CGCG	1 cut(s)	602
BstV1I	GCAGC	9 cut(s)	604, 608, 839, 1098, 1621, 1715, 2134, 2215, 2218
BstV2I	GAAGAC	2 cut(s)	302, 1001
BstX2I	RGATCY	4 cut(s)	346, 943, 1348, 1496
BstYI	RGATCY	4 cut(s)	346, 943, 1348, 1496
BstZ17I	GTATAC	1 cut(s)	1288
Bsu36I	CCTNAGG	1 cut(s)	2394
BsuI	GTATCC	1 cut(s)	283
BtgI	CCRYGG	2 cut(s)	1384, 1837
BtsCI	GGATG	5 cut(s)	357, 759, 1750, 1947, 2349
BtsIMutI	CAGTG	8 cut(s)	288, 366, 567, 657, 840, 1356, 1824, 2070
Cac8I	GCNNGC	6 cut(s)	919, 1109, 1296, 1664, 1806, 1870
CciI	TCATGA	2 cut(s)	1146, 1921
CfoI	GCGC	3 cut(s)	765, 923, 1810
Cfr10I	RCCGGY	2 cut(s)	1107, 2414
Cfr13I	GGNCC	9 cut(s)	218, 476, 908, 1033, 1834, 1927, 2211, 2267, 2268
Cfr9I	CCCGGG	1 cut(s)	1960
Csp6I	GTAC	6 cut(s)	432, 973, 1311, 1327, 1389, 2327
CspCI	CAANNNNNGTGG	2 cut(s)	556, 591
CviQI	GTAC	6 cut(s)	432, 973, 1311, 1327, 1389, 2327
DdeI	CTNAG	9 cut(s)	170, 351, 549, 1023, 1359, 1613, 1953, 1990, 2394
DpnI	GATC	9 cut(s)	348, 605, 945, 1215, 1350, 1412, 1498, 1826, 1920
DpnII	GATC	9 cut(s)	346, 603, 943, 1213, 1348, 1410, 1496, 1824, 1918
DraI	TTTAAA	1 cut(s)	748
DriI	GACNNNNNGTC	1 cut(s)	1460
EaeI	YGGCCR	3 cut(s)	577, 585, 661
Eam1104I	CTCTTC	2 cut(s)	320, 1654
Eam1105I	GACNNNNNGTC	1 cut(s)	1460
EarI	CTCTTC	2 cut(s)	320, 1654
EciI	GGCGGA	1 cut(s)	350
Ecl136II	GAGCTC	4 cut(s)	553, 1022, 1171, 1363
Eco130I	CCWWGG	1 cut(s)	1843
Eco24I	GRGCYC	5 cut(s)	555, 1024, 1173, 1365, 2271
Eco47I	GGWCC	2 cut(s)	476, 1834
Eco47III	AGCGCT	1 cut(s)	764
Eco53kI	GAGCTC	4 cut(s)	553, 1022, 1171, 1363
Eco57I	CTGAAG	3 cut(s)	207, 1991, 2037
Eco81I	CCTNAGG	1 cut(s)	2394
Eco88I	CYCGRG	2 cut(s)	1136, 1960
EcoICRI	GAGCTC	4 cut(s)	553, 1022, 1171, 1363
EcoO109I	RGGNCCY	3 cut(s)	1033, 1927, 2267
EcoRI	GAATTC	1 cut(s)	855
EcoRII	CCWGG	2 cut(s)	672, 2356
EcoT14I	CCWWGG	1 cut(s)	1843
EcoT22I	ATGCAT	2 cut(s)	1476, 1668
EcoT38I	GRGCYC	5 cut(s)	555, 1024, 1173, 1365, 2271
ErhI	CCWWGG	1 cut(s)	1843
FaqI	GGGAC	4 cut(s)	753, 881, 1450, 1703
FauI	CCCGC	1 cut(s)	2004
FbaI	TGATCA	3 cut(s)	1213, 1410, 1918
FblI	GTMKAC	1 cut(s)	1287
FokI	GGATG	5 cut(s)	344, 766, 1757, 1954, 2356
FriOI	GRGCYC	5 cut(s)	555, 1024, 1173, 1365, 2271
FspBI	CTAG	2 cut(s)	984, 1659
GlaI	GCGC	3 cut(s)	764, 922, 1809
GsaI	CCCAGC	2 cut(s)	834, 2267
GsuI	CTGGAG	2 cut(s)	1349, 2277
HaeII	RGCGCY	2 cut(s)	766, 924
HapII	CCGG	4 cut(s)	1108, 1961, 2258, 2415
HhaI	GCGC	3 cut(s)	765, 923, 1810
Hin6I	GCGC	3 cut(s)	763, 921, 1808
HinP1I	GCGC	3 cut(s)	763, 921, 1808
HincII	GTYRAC	2 cut(s)	1786, 2286
HindII	GTYRAC	2 cut(s)	1786, 2286
HindIII	AAGCTT	1 cut(s)	557
HinfI	GANTC	8 cut(s)	145, 625, 888, 1507, 1565, 1615, 2167, 2405
HpaII	CCGG	4 cut(s)	1108, 1961, 2258, 2415
HphI	GGTGA	4 cut(s)	86, 788, 1570, 1768
Hpy166II	GTNNAC	9 cut(s)	779, 1288, 1519, 1786, 1834, 1984, 2029, 2068, 2286
Hpy188I	TCNGA	5 cut(s)	887, 1070, 1318, 1512, 2410
Hpy8I	GTNNAC	9 cut(s)	779, 1288, 1519, 1786, 1834, 1984, 2029, 2068, 2286
Hpy99I	CGWCG	2 cut(s)	1982, 2387
HpyCH4III	ACNGT	7 cut(s)	19, 283, 1087, 1388, 1838, 1978, 2255
HpyCH4IV	ACGT	2 cut(s)	1220, 1601
HpyF10VI	GCNNNNNNNGC	3 cut(s)	35, 593, 824
HpyF3I	CTNAG	9 cut(s)	170, 351, 549, 1023, 1359, 1613, 1953, 1990, 2394
HpySE526I	ACGT	2 cut(s)	1220, 1601
HspAI	GCGC	3 cut(s)	763, 921, 1808
Kpn2I	TCCGGA	1 cut(s)	2257
KroI	GCCGGC	1 cut(s)	1107
KroNI	GCCGGC	1 cut(s)	1109
Ksp22I	TGATCA	3 cut(s)	1213, 1410, 1918
Kzo9I	GATC	9 cut(s)	346, 603, 943, 1213, 1348, 1410, 1496, 1824, 1918
LmnI	GCTCC	6 cut(s)	872, 1019, 1168, 1368, 1483, 2260
Lsp1109I	GCAGC	9 cut(s)	604, 608, 839, 1098, 1621, 1715, 2134, 2215, 2218
LweI	GCATC	3 cut(s)	1483, 1932, 2174
MaeI	CTAG	2 cut(s)	984, 1659
MaeII	ACGT	2 cut(s)	1220, 1601
MaeIII	GTNAC	6 cut(s)	130, 233, 838, 1558, 1756, 1964
MalI	GATC	9 cut(s)	348, 605, 945, 1215, 1350, 1412, 1498, 1826, 1920
MboI	GATC	9 cut(s)	346, 603, 943, 1213, 1348, 1410, 1496, 1824, 1918
MfeI	CAATTG	1 cut(s)	1335
MflI	RGATCY	4 cut(s)	346, 943, 1348, 1496
MhlI	GDGCHC	7 cut(s)	555, 885, 1024, 1173, 1365, 1959, 2271
MlsI	TGGCCA	1 cut(s)	587
MluNI	TGGCCA	1 cut(s)	587
MlyI	GAGTC	1 cut(s)	1624
MmeI	TCCRAC	3 cut(s)	267, 324, 1722
Mox20I	TGGCCA	1 cut(s)	587
Mph1103I	ATGCAT	2 cut(s)	1476, 1668
MroI	TCCGGA	1 cut(s)	2257
MroNI	GCCGGC	1 cut(s)	1107
MscI	TGGCCA	1 cut(s)	587
MseI	TTAA	6 cut(s)	563, 747, 1182, 1488, 1973, 2178
MslI	CAYNNNNRTG	1 cut(s)	500
Msp20I	TGGCCA	1 cut(s)	587
MspA1I	CMGCKG	3 cut(s)	830, 1612, 2011
MspI	CCGG	4 cut(s)	1108, 1961, 2258, 2415
MspR9I	CCNGG	4 cut(s)	674, 1961, 1962, 2358
MunI	CAATTG	1 cut(s)	1335
MvaI	CCWGG	2 cut(s)	674, 2358
MvnI	CGCG	1 cut(s)	602
MwoI	GCNNNNNNNGC	3 cut(s)	35, 593, 824
NaeI	GCCGGC	1 cut(s)	1109
NciI	CCSGG	2 cut(s)	1961, 1962
NdeII	GATC	9 cut(s)	346, 603, 943, 1213, 1348, 1410, 1496, 1824, 1918
NgoMIV	GCCGGC	1 cut(s)	1107
NlaIV	GGNNCC	7 cut(s)	348, 1034, 1142, 1397, 2100, 2268, 2269
NmuCI	GTSAC	4 cut(s)	233, 838, 1558, 1756
NsiI	ATGCAT	2 cut(s)	1476, 1668
NspI	RCATGY	3 cut(s)	33, 1666, 1808
PaeI	GCATGC	2 cut(s)	1666, 1808
PagI	TCATGA	2 cut(s)	1146, 1921
PdiI	GCCGGC	1 cut(s)	1109
PfeI	GAWTC	7 cut(s)	145, 625, 888, 1507, 1565, 2167, 2405
PflMI	CCANNNNNTGG	1 cut(s)	1741
PleI	GAGTC	1 cut(s)	1623
PpsI	GAGTC	1 cut(s)	1623
PsiI	TTATAA	1 cut(s)	1323
Psp124BI	GAGCTC	4 cut(s)	555, 1024, 1173, 1365
Psp1406I	AACGTT	2 cut(s)	1220, 1601
Psp6I	CCWGG	2 cut(s)	672, 2356
PspFI	CCCAGC	2 cut(s)	830, 2263
PspGI	CCWGG	2 cut(s)	672, 2356
PspN4I	GGNNCC	7 cut(s)	348, 1034, 1142, 1397, 2100, 2268, 2269
PspOMI	GGGCCC	1 cut(s)	2267
PspPI	GGNCC	9 cut(s)	218, 476, 908, 1033, 1834, 1927, 2211, 2267, 2268
PsrI	GAACNNNNNNTAC	2 cut(s)	717, 749
PstI	CTGCAG	1 cut(s)	2152
PsuI	RGATCY	4 cut(s)	346, 943, 1348, 1496
PvuII	CAGCTG	2 cut(s)	830, 1612
RsaI	GTAC	6 cut(s)	433, 974, 1312, 1328, 1390, 2328
RsaNI	GTAC	6 cut(s)	432, 973, 1311, 1327, 1389, 2327
RseI	CAYNNNNRTG	1 cut(s)	500
SacI	GAGCTC	4 cut(s)	555, 1024, 1173, 1365
SaqAI	TTAA	6 cut(s)	563, 747, 1182, 1488, 1973, 2178
Sau3AI	GATC	9 cut(s)	346, 603, 943, 1213, 1348, 1410, 1496, 1824, 1918
Sau96I	GGNCC	9 cut(s)	218, 476, 908, 1033, 1834, 1927, 2211, 2267, 2268
SchI	GAGTC	1 cut(s)	1624
ScrFI	CCNGG	4 cut(s)	674, 1961, 1962, 2358
SduI	GDGCHC	7 cut(s)	555, 885, 1024, 1173, 1365, 1959, 2271
SfaNI	GCATC	3 cut(s)	1483, 1932, 2174
SfcI	CTRYAG	3 cut(s)	1272, 2148, 2307
SinI	GGWCC	2 cut(s)	476, 1834
SmaI	CCCGGG	1 cut(s)	1962
SmiMI	CAYNNNNRTG	1 cut(s)	500
SmlI	CTYRAG	2 cut(s)	554, 1799
SmoI	CTYRAG	2 cut(s)	554, 1799
SphI	GCATGC	2 cut(s)	1666, 1808
SsiI	CCGC	4 cut(s)	361, 364, 600, 2011
SspI	AATATT	2 cut(s)	210, 261
SspMI	CTAG	2 cut(s)	984, 1659
SstI	GAGCTC	4 cut(s)	555, 1024, 1173, 1365
StyD4I	CCNGG	4 cut(s)	672, 1959, 1960, 2356
StyI	CCWWGG	1 cut(s)	1843
TaaI	ACNGT	7 cut(s)	19, 283, 1087, 1388, 1838, 1978, 2255
TaiI	ACGT	2 cut(s)	1223, 1604
TaqI	TCGA	7 cut(s)	163, 252, 736, 1422, 1618, 1765, 2375
TaqII	GACCGA	1 cut(s)	2305
TatI	WGTACW	4 cut(s)	972, 1310, 1326, 2326
TauI	GCSGC	2 cut(s)	366, 602
TfiI	GAWTC	7 cut(s)	145, 625, 888, 1507, 1565, 2167, 2405
Tru1I	TTAA	6 cut(s)	563, 747, 1182, 1488, 1973, 2178
Tru9I	TTAA	6 cut(s)	563, 747, 1182, 1488, 1973, 2178
TscAI	CASTG	8 cut(s)	288, 373, 574, 664, 847, 1363, 1824, 2070
TseFI	GTSAC	4 cut(s)	233, 838, 1558, 1756
TseI	GCWGC	9 cut(s)	596, 617, 827, 1111, 1609, 1703, 2147, 2228, 2231
Tsp45I	GTSAC	4 cut(s)	233, 838, 1558, 1756
TspDTI	ATGAA	8 cut(s)	59, 316, 484, 848, 1004, 1163, 1249, 2068
TspGWI	ACGGA	4 cut(s)	157, 1577, 1619, 1642
TspMI	CCCGGG	1 cut(s)	1960
TspRI	CASTG	8 cut(s)	288, 373, 574, 664, 847, 1363, 1824, 2070
Van91I	CCANNNNNTGG	1 cut(s)	1741
VpaK11BI	GGWCC	2 cut(s)	476, 1834
XapI	RAATTY	1 cut(s)	855
XceI	RCATGY	3 cut(s)	33, 1666, 1808
XcmI	CCANNNNNNNNNTGG	2 cut(s)	747, 1742
XmaI	CCCGGG	1 cut(s)	1960
XmiI	GTMKAC	1 cut(s)	1287
XspI	CTAG	2 cut(s)	984, 1659
Zsp2I	ATGCAT	2 cut(s)	1476, 1668

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.