Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005981

Physical Location & Seq

Genomic Coordinates Reverse (-)

12662561 .. 12667262

Gene Structure 4702 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G24520.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2079 bp

ATGACTTCTGCTTTGTATGCTTCCGATTTGTTTAAGCAGCTCAAGTCAATTATGGGGACAGATTCGTTATCCGACGATGTTGTACTTGTGATTGCAACGACGTCTTTGGCACTAGTAGCTGGATTTGTGGTGTTGTTATGGAAGAAAACGACGGCGGATCGGAGCGGGGAGCTGAAGCCTTTGATGATCCCTAAGTCTCTTATGGCTAAGGACGAGGATGATGATTTGGATTTGGGATCCGGGAAGACTAGAGTCTCTATCTTCTTCGGTACGCAGACTGGAACAGCTGAGGGATTTGCTAAGGCATTATCCGAAGAAATCAAAGCGAGATATGAAAAAGCAGCAGTCAAAGTCATTGACTTGGATGACTATGCTGCCGATGATGACCAGTATGAAGAGAAATTGAAGAAGGAAACTTTGGCATTTTTCTGTGTTGCTACTTATGGAGATGGAGAGCCTACTGACAATGCTGCCAGATTTTACAAATGGTTTACGGAGGAAAATGAACGGGATATAAAGCTTCAACAACTAGCATATGGTGTGTTTGCTCTTGGTAATCGCCAATATGAACATTTTAATAAGATCGGGATAGTTCTTGATGAAGAGTTATGTAAGAAAGGTGCAAAGCGTCTTATTGAAGTCGGTCTAGGAGATGATGATCAGAGCATTGAGGATGATTTTAATGCCTGGAAAGAATCACTATGGTCTGAGCTAGACAAGCTCCTCAAAGACGAGGATGATAAAAGTGTGGCAACTCCTTATACAGCTGTTATTCCTGAATACCGGGTGGTGACTCATGATCCTCGGTTTACAACTCAAAAATCAATGGAATCAAATGTGGCCAATGGAAATACTACTATTGACATTCATCATCCCTGCAGAGTTGATGTTGCTGTGCAGAAGGAGCTTCACACACATGAATCTGATCGGTCTTGCATTCATCTCGAGTTCGACATATCCAGGACGGGTATTACATATGAAACAGGTGACCATGTAGGTGTATATGCTGAAAATCATGTTGAAATAGTTGAAGAAGCTGGAAAATTGCTTGGCCACTCTTTAGATTTAGTATTTTCCATACATGCTGACAAGGAAGATGGCTCCCCATTGGAAAGCGCAGTGCCGCCTCCTTTCCCTGGTCCATGCACACTTGGGACTGGTTTGGCAAGATACGCAGACCTTTTGAACCCTCCTCGAAAGTCTGCGTTAGTTGCCTTGGCGGCCTATGCCACTGAACCAAGTGAAGCCGAGAAACTTAAGCACCTGACATCACCTGATGGAAAGGATGAGTACTCACAATGGATTGTTGCAAGTCAGAGAAGTCTTTTAGAGGTGATGGCTGCTTTTCCATCTGCAAAACCCCCACTAGGTGTATTTTTTGCTGCAATAGCTCCTCGTCTACAACCTCGTTACTACTCCATCTCATCCTCGCCAAGATTGGCGCCAAGTAGAGTTCATGTTACATCCGCACTAGTATATGGTCCAACTCCTACTGGTAGAATCCACAAGGGTGTGTGTTCTACGTGGATGAAGAATGCAGTTCCTGCGGAGAAAAGTCATGAATGTAGTGGAGCCCCAATCTTTATTCGAGCATCTAATTTCAAGTTACCATCCAACCCTTCAACTCCAATCGTTATGGTGGGACCTGGGACTGGGCTGGCACCTTTTAGAGGTTTTCTGCAGGAAAGGATGGCACTAAAAGAAGATGGAGAAGAACTAGGTTCATCTTTGCTCTTCTTTGGGTGTAGAAATCGACAGATGGACTTTATATACGAGGATGAGCTCAATAATTTTGTTGATCAAGGCGTAATATCTGAGCTCATCATGGCATTCTCCCGTGAAGGAGCTCAGAAGGAGTATGTTCAACATAAGATGATGGAGAAGGCAGCACAAGTTTGGGATCTAATAAAGGAAGAAGGATATCTCTATGTATGCGGTGATGCTAAGGGCATGGCGAGGGACGTCCACCGAACTCTACACACCATTGTTCAGGAGCAGGAAGGTGTGAGTTCGTCAGAGGCAGAGGCTATAGTTAAGAAACTTCAAACCGAAGGAAGATACCTCAGAGATGTCTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0003958 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0006725 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009698 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0012505 GO:0016491 GO:0016651 GO:0016653 GO:0019748 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0055114 GO:0071704 GO:0097305 GO:1901360 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_1 Flavodoxin_1 NAD_binding_1

Protein Analysis

692

Amino Acids

76.76

Weight (kDa)

5.11

Isoelectric Point (pI)

33.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Flavodoxin_1	PF00258	87 - 226	4.8e-33	Flavodoxin
FAD_binding_1	PF00667	284 - 505	2.4e-71	FAD binding domain
NAD_binding_1	PF00175	542 - 652	1.6e-15	Oxidoreductase NAD-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005981)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24520 AT4G24520
fragaria_vesca	FvH4_5g34830 FvH4_5g34830 FvH4_5g34830 FvH4_5g34830 FvH4_5g34830
malus_domestica	MD08G1199100.v1.1 MD15G1386100.v1.1
prunus_persica	Prupe.1G532800_v2.0.a1 Prupe.1G532800_v2.0.a1 Prupe.1G532800_v2.0.a1
pyrus_communis	pycom08g17290 pycom15g34630
rosa_chinensis	RchiOBHm_Chr7g0235511
rosa_laevigata	RLG00000001153
rosa_multiflora	Rmu_sc0002617.1_g000018 Rmu_sc0002892.1_g000021 Rmu_sc0039003.1_g000003 Rmu_sc0042677.1_g000001
rosa_roxburghii	Rroxscaffold_3G00225940
rosa_rugosa	Rorug07G0291400
rosa_samantha	Rh7AG446500 Rh7BG418200 Rh7CG466500 Rh7DG435200
rosa_wichuraiana	Rw7G037070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	104, 1965
AccB1I	GGYRCC	2 cut(s)	1441, 1660
AccBSI	CCGCTC	1 cut(s)	165
AccI	GTMKAC	1 cut(s)	1399
AciI	CCGC	7 cut(s)	155, 165, 1124, 1220, 1467, 1547, 1935
AclWI	GGATC	6 cut(s)	165, 181, 231, 244, 794, 1908
AcoI	YGGCCR	2 cut(s)	840, 1051
AcuI	CTGAAG	1 cut(s)	194
AcyI	GRCGYC	3 cut(s)	101, 1442, 1962
AdeI	CACNNNGTG	1 cut(s)	1370
AfaI	GTAC	3 cut(s)	84, 271, 1292
AfiI	CCNNNNNNNGG	4 cut(s)	1136, 1367, 1652, 1670
AflII	CTTAAG	1 cut(s)	1256
AhlI	ACTAGT	2 cut(s)	112, 1471
AjnI	CCWGG	4 cut(s)	686, 959, 1135, 1645
AjuI	GAANNNNNNNTTGG	2 cut(s)	401, 433
AleI	CACNNNNGTG	1 cut(s)	1509
Alw21I	GWGCWC	3 cut(s)	1785, 1821, 1849
Alw26I	GTCTC	2 cut(s)	201, 259
AlwI	GGATC	6 cut(s)	165, 181, 231, 244, 794, 1908
AlwNI	CAGNNNCTG	1 cut(s)	1544
Ama87I	CYCGRG	1 cut(s)	944
AoxI	GGCC	3 cut(s)	840, 1051, 1221
ApeKI	GCWGC	7 cut(s)	37, 341, 374, 470, 1340, 1382, 1886
ArsI	GACNNNNNNTTYG	1 cut(s)	27
AspLEI	GCGC	2 cut(s)	1118, 1444
AspS9I	GGNCC	3 cut(s)	1139, 1481, 1643
AsuC2I	CCSGG	2 cut(s)	241, 785
AsuHPI	GGTGA	5 cut(s)	802, 998, 1263, 1345, 1949
AvaI	CYCGRG	1 cut(s)	944
AvaII	GGWCC	3 cut(s)	1139, 1481, 1643
BalI	TGGCCA	2 cut(s)	842, 1053
BamHI	GGATCC	1 cut(s)	236
BanI	GGYRCC	2 cut(s)	1441, 1660
BanII	GRGCYC	4 cut(s)	1576, 1785, 1821, 1849
BbsI	GAAGAC	1 cut(s)	251
Bbv12I	GWGCWC	3 cut(s)	1785, 1821, 1849
BbvCI	CCTCAGC	1 cut(s)	288
BbvI	GCAGC	7 cut(s)	49, 353, 361, 457, 1327, 1369, 1898
BceAI	ACGGC	1 cut(s)	168
BcgI	CGANNNNNNTGC	2 cut(s)	925, 959
BciT130I	CCWGG	4 cut(s)	688, 961, 1137, 1647
BclI	TGATCA	2 cut(s)	658, 1798
BcnI	CCSGG	2 cut(s)	241, 785
BcoDI	GTCTC	2 cut(s)	201, 259
BcuI	ACTAGT	2 cut(s)	112, 1471
BfaI	CTAG	8 cut(s)	113, 249, 530, 647, 713, 1367, 1472, 1718
BfmI	CTRYAG	3 cut(s)	877, 1679, 2028
BfoI	RGCGCY	1 cut(s)	1445
BfrI	CTTAAG	1 cut(s)	1256
BglI	GCCNNNNNGGC	1 cut(s)	1220
BisI	GCNGC	9 cut(s)	38, 342, 375, 471, 1124, 1221, 1341, 1383, 1887
BlsI	GCNGC	9 cut(s)	39, 343, 376, 472, 1125, 1222, 1342, 1384, 1888
BmcAI	AGTACT	1 cut(s)	1292
Bme1390I	CCNGG	6 cut(s)	241, 688, 785, 961, 1137, 1647
Bme18I	GGWCC	3 cut(s)	1139, 1481, 1643
BmeT110I	CYCGRG	1 cut(s)	944
BmgT120I	GGNCC	3 cut(s)	1139, 1481, 1643
BmiI	GGNNCC	6 cut(s)	238, 1102, 1443, 1573, 1644, 1662
BmrFI	CCNGG	6 cut(s)	241, 688, 785, 961, 1137, 1647
BmrI	ACTGGG	1 cut(s)	1662
BmsI	GCATC	2 cut(s)	1601, 1930
BmuI	ACTGGG	1 cut(s)	1662
BoxI	GACNNNNGTC	1 cut(s)	251
BpiI	GAAGAC	1 cut(s)	251
Bpu10I	CCTNAGC	4 cut(s)	207, 288, 300, 1944
BpuEI	CTTGAG	1 cut(s)	26
BpuMI	CCSGG	2 cut(s)	241, 785
BsaAI	YACGTR	1 cut(s)	1524
BsaBI	GATNNNNATC	1 cut(s)	657
BsaHI	GRCGYC	3 cut(s)	101, 1442, 1962
BsaJI	CCNNGG	4 cut(s)	803, 1135, 1215, 1646
Bsc4I	CCNNNNNNNGG	4 cut(s)	1136, 1367, 1652, 1670
Bse1I	ACTGG	5 cut(s)	283, 388, 1162, 1498, 1657
Bse8I	GATNNNNATC	1 cut(s)	657
BseBI	CCWGG	4 cut(s)	688, 961, 1137, 1647
BseDI	CCNNGG	4 cut(s)	803, 1135, 1215, 1646
BseJI	GATNNNNATC	1 cut(s)	657
BseLI	CCNNNNNNNGG	4 cut(s)	1136, 1367, 1652, 1670
BseMII	CTCAG	5 cut(s)	279, 699, 1806, 1862, 2077
BseNI	ACTGG	5 cut(s)	283, 388, 1162, 1498, 1657
BseRI	GAGGAG	3 cut(s)	713, 1182, 1383
BseXI	GCAGC	7 cut(s)	49, 353, 361, 457, 1327, 1369, 1898
BsgI	GTGCAG	1 cut(s)	917
BshFI	GGCC	3 cut(s)	842, 1053, 1223
BshNI	GGYRCC	2 cut(s)	1441, 1660
BsiHKAI	GWGCWC	3 cut(s)	1785, 1821, 1849
BsiHKCI	CYCGRG	1 cut(s)	944
BsiSI	CCGG	2 cut(s)	240, 784
BslFI	GGGAC	5 cut(s)	70, 1168, 1656, 1663, 1973
BslI	CCNNNNNNNGG	4 cut(s)	1136, 1367, 1652, 1670
BsmAI	GTCTC	2 cut(s)	201, 259
BsmFI	GGGAC	5 cut(s)	70, 1168, 1656, 1663, 1973
BsmI	GAATGC	3 cut(s)	936, 1540, 1829
BsnI	GGCC	3 cut(s)	842, 1053, 1223
BsoBI	CYCGRG	1 cut(s)	944
Bsp1286I	GDGCHC	4 cut(s)	1576, 1785, 1821, 1849
Bsp143I	GATC	9 cut(s)	157, 186, 236, 582, 658, 799, 925, 1798, 1900
BspACI	CCGC	7 cut(s)	155, 165, 1124, 1220, 1467, 1547, 1935
BspANI	GGCC	3 cut(s)	842, 1053, 1223
BspCNI	CTCAG	5 cut(s)	280, 700, 1807, 1861, 2076
BspHI	TCATGA	2 cut(s)	796, 1558
BspLI	GGNNCC	6 cut(s)	238, 1102, 1443, 1573, 1644, 1662
BspMAI	CTGCAG	2 cut(s)	881, 1683
BspPI	GGATC	6 cut(s)	165, 181, 231, 244, 794, 1908
BspQI	GCTCTTC	1 cut(s)	1739
BspT107I	GGYRCC	2 cut(s)	1441, 1660
BspTI	CTTAAG	1 cut(s)	1256
BsrBI	CCGCTC	1 cut(s)	165
BsrI	ACTGG	5 cut(s)	283, 388, 1162, 1498, 1657
BssECI	CCNNGG	4 cut(s)	803, 1135, 1215, 1646
BssMI	GATC	9 cut(s)	157, 186, 236, 582, 658, 799, 925, 1798, 1900
BssNI	GRCGYC	3 cut(s)	101, 1442, 1962
BssT1I	CCWWGG	1 cut(s)	1215
Bst2UI	CCWGG	4 cut(s)	688, 961, 1137, 1647
Bst6I	CTCTTC	3 cut(s)	390, 597, 1739
BstACI	GRCGYC	3 cut(s)	101, 1442, 1962
BstAFI	CTTAAG	1 cut(s)	1256
BstAPI	GCANNNNNTGC	1 cut(s)	1544
BstBAI	YACGTR	1 cut(s)	1524
BstC8I	GCNNGC	1 cut(s)	1659
BstDEI	CTNAG	9 cut(s)	192, 207, 288, 300, 708, 1815, 1848, 1944, 2063
BstEII	GGTNACC	1 cut(s)	986
BstENI	CCTNNNNNAGG	1 cut(s)	1668
BstH2I	RGCGCY	1 cut(s)	1445
BstHHI	GCGC	2 cut(s)	1118, 1444
BstKTI	GATC	9 cut(s)	160, 189, 239, 585, 661, 802, 928, 1801, 1903
BstMAI	GTCTC	2 cut(s)	201, 259
BstMBI	GATC	9 cut(s)	157, 186, 236, 582, 658, 799, 925, 1798, 1900
BstNI	CCWGG	4 cut(s)	688, 961, 1137, 1647
BstNSI	RCATGY	1 cut(s)	1085
BstPAI	GACNNNNGTC	1 cut(s)	251
BstPI	GGTNACC	1 cut(s)	986
BstSCI	CCNGG	6 cut(s)	239, 686, 783, 959, 1135, 1645
BstSFI	CTRYAG	3 cut(s)	877, 1679, 2028
BstV1I	GCAGC	7 cut(s)	49, 353, 361, 457, 1327, 1369, 1898
BstV2I	GAAGAC	1 cut(s)	251
BstX2I	RGATCY	2 cut(s)	236, 1900
BstYI	RGATCY	2 cut(s)	236, 1900
BsuRI	GGCC	3 cut(s)	842, 1053, 1223
BtsI	GCAGTG	1 cut(s)	1125
BtsIMutI	CAGTG	2 cut(s)	1125, 1230
Cac8I	GCNNGC	1 cut(s)	1659
CaiI	CAGNNNCTG	1 cut(s)	1544
CciI	TCATGA	2 cut(s)	796, 1558
CfoI	GCGC	2 cut(s)	1118, 1444
Cfr13I	GGNCC	3 cut(s)	1139, 1481, 1643
CseI	GACGC	1 cut(s)	617
Csp6I	GTAC	3 cut(s)	83, 270, 1291
CviQI	GTAC	3 cut(s)	83, 270, 1291
DdeI	CTNAG	9 cut(s)	192, 207, 288, 300, 708, 1815, 1848, 1944, 2063
DinI	GGCGCC	1 cut(s)	1443
DpnI	GATC	9 cut(s)	159, 188, 238, 584, 660, 801, 927, 1800, 1902
DpnII	GATC	9 cut(s)	157, 186, 236, 582, 658, 799, 925, 1798, 1900
DraIII	CACNNNGTG	1 cut(s)	1370
EaeI	YGGCCR	2 cut(s)	840, 1051
Eam1104I	CTCTTC	3 cut(s)	390, 597, 1739
EarI	CTCTTC	3 cut(s)	390, 597, 1739
EciI	GGCGGA	1 cut(s)	170
Ecl136II	GAGCTC	3 cut(s)	1783, 1819, 1847
Eco130I	CCWWGG	1 cut(s)	1215
Eco24I	GRGCYC	4 cut(s)	1576, 1785, 1821, 1849
Eco32I	GATATC	1 cut(s)	1922
Eco47I	GGWCC	3 cut(s)	1139, 1481, 1643
Eco53kI	GAGCTC	3 cut(s)	1783, 1819, 1847
Eco57I	CTGAAG	1 cut(s)	194
Eco88I	CYCGRG	1 cut(s)	944
Eco91I	GGTNACC	1 cut(s)	986
EcoICRI	GAGCTC	3 cut(s)	1783, 1819, 1847
EcoNI	CCTNNNNNAGG	1 cut(s)	1668
EcoO109I	RGGNCCY	1 cut(s)	1643
EcoO65I	GGTNACC	1 cut(s)	986
EcoRII	CCWGG	4 cut(s)	686, 959, 1135, 1645
EcoRV	GATATC	1 cut(s)	1922
EcoT14I	CCWWGG	1 cut(s)	1215
EcoT38I	GRGCYC	4 cut(s)	1576, 1785, 1821, 1849
EgeI	GGCGCC	1 cut(s)	1443
EheI	GGCGCC	1 cut(s)	1443
ErhI	CCWWGG	1 cut(s)	1215
FaqI	GGGAC	5 cut(s)	70, 1168, 1656, 1663, 1973
FauI	CCCGC	1 cut(s)	158
FauNDI	CATATG	2 cut(s)	535, 976
FbaI	TGATCA	2 cut(s)	658, 1798
FblI	GTMKAC	1 cut(s)	1399
Fnu4HI	GCNGC	9 cut(s)	38, 342, 375, 471, 1124, 1221, 1341, 1383, 1887
FriOI	GRGCYC	4 cut(s)	1576, 1785, 1821, 1849
Fsp4HI	GCNGC	9 cut(s)	38, 342, 375, 471, 1124, 1221, 1341, 1383, 1887
FspBI	CTAG	8 cut(s)	113, 249, 530, 647, 713, 1367, 1472, 1718
GlaI	GCGC	2 cut(s)	1117, 1443
GluI	GCNGC	9 cut(s)	38, 342, 375, 471, 1124, 1221, 1341, 1383, 1887
HaeII	RGCGCY	1 cut(s)	1445
HaeIII	GGCC	3 cut(s)	842, 1053, 1223
HapII	CCGG	2 cut(s)	240, 784
HgaI	GACGC	1 cut(s)	617
HhaI	GCGC	2 cut(s)	1118, 1444
Hin1I	GRCGYC	3 cut(s)	101, 1442, 1962
Hin6I	GCGC	2 cut(s)	1116, 1442
HinP1I	GCGC	2 cut(s)	1116, 1442
HindIII	AAGCTT	1 cut(s)	518
HinfI	GANTC	7 cut(s)	62, 252, 695, 793, 830, 920, 1500
HpaII	CCGG	2 cut(s)	240, 784
HphI	GGTGA	5 cut(s)	802, 998, 1263, 1345, 1949
Hpy166II	GTNNAC	4 cut(s)	492, 810, 1400, 1966
Hpy188III	TCNNGA	7 cut(s)	586, 596, 776, 797, 944, 1559, 1991
Hpy8I	GTNNAC	4 cut(s)	492, 810, 1400, 1966
Hpy99I	CGWCG	3 cut(s)	77, 103, 154
HpyAV	CCTTC	9 cut(s)	403, 895, 1629, 1835, 1846, 1876, 1910, 1994, 2045
HpyCH4IV	ACGT	3 cut(s)	101, 1523, 1962
HpyF3I	CTNAG	9 cut(s)	192, 207, 288, 300, 708, 1815, 1848, 1944, 2063
HpySE526I	ACGT	3 cut(s)	101, 1523, 1962
Hsp92I	GRCGYC	3 cut(s)	101, 1442, 1962
HspAI	GCGC	2 cut(s)	1116, 1442
KasI	GGCGCC	1 cut(s)	1441
Ksp22I	TGATCA	2 cut(s)	658, 1798
Kzo9I	GATC	9 cut(s)	157, 186, 236, 582, 658, 799, 925, 1798, 1900
LguI	GCTCTTC	1 cut(s)	1739
LmnI	GCTCC	9 cut(s)	162, 169, 726, 904, 1106, 1396, 1571, 1844, 1993
Lsp1109I	GCAGC	7 cut(s)	49, 353, 361, 457, 1327, 1369, 1898
LweI	GCATC	2 cut(s)	1601, 1930
MaeI	CTAG	8 cut(s)	113, 249, 530, 647, 713, 1367, 1472, 1718
MaeII	ACGT	3 cut(s)	101, 1523, 1962
MaeIII	GTNAC	5 cut(s)	790, 986, 1409, 1459, 1605
MalI	GATC	9 cut(s)	159, 188, 238, 584, 660, 801, 927, 1800, 1902
MbiI	CCGCTC	1 cut(s)	165
MboI	GATC	9 cut(s)	157, 186, 236, 582, 658, 799, 925, 1798, 1900
MflI	RGATCY	2 cut(s)	236, 1900
MhlI	GDGCHC	4 cut(s)	1576, 1785, 1821, 1849
MlsI	TGGCCA	2 cut(s)	842, 1053
MluCI	AATT	5 cut(s)	48, 401, 1043, 1597, 1789
MluNI	TGGCCA	2 cut(s)	842, 1053
Mly113I	GGCGCC	1 cut(s)	1442
MlyI	GAGTC	2 cut(s)	261, 787
MmeI	TCCRAC	3 cut(s)	96, 1508, 1638
Mox20I	TGGCCA	2 cut(s)	842, 1053
MscI	TGGCCA	2 cut(s)	842, 1053
MseI	TTAA	5 cut(s)	33, 576, 681, 1257, 2034
MslI	CAYNNNNRTG	3 cut(s)	915, 996, 1509
Msp20I	TGGCCA	2 cut(s)	842, 1053
MspA1I	CMGCKG	2 cut(s)	287, 767
MspCI	CTTAAG	1 cut(s)	1256
MspI	CCGG	2 cut(s)	240, 784
MspR9I	CCNGG	6 cut(s)	241, 688, 785, 961, 1137, 1647
Mva1269I	GAATGC	3 cut(s)	936, 1540, 1829
MvaI	CCWGG	4 cut(s)	688, 961, 1137, 1647
NarI	GGCGCC	1 cut(s)	1442
NciI	CCSGG	2 cut(s)	241, 785
NdeI	CATATG	2 cut(s)	535, 976
NdeII	GATC	9 cut(s)	157, 186, 236, 582, 658, 799, 925, 1798, 1900
NlaIV	GGNNCC	6 cut(s)	238, 1102, 1443, 1573, 1644, 1662
NmeAIII	GCCGAG	1 cut(s)	1273
NmuCI	GTSAC	2 cut(s)	790, 986
NspI	RCATGY	1 cut(s)	1085
OliI	CACNNNNGTG	1 cut(s)	1509
PaeR7I	CTCGAG	1 cut(s)	944
PagI	TCATGA	2 cut(s)	796, 1558
PciSI	GCTCTTC	1 cut(s)	1739
PctI	GAATGC	3 cut(s)	936, 1540, 1829
PfeI	GAWTC	5 cut(s)	62, 695, 830, 920, 1500
PfoI	TCCNGGA	2 cut(s)	239, 959
PkrI	GCNGC	9 cut(s)	39, 343, 376, 472, 1125, 1222, 1342, 1384, 1888
PleI	GAGTC	2 cut(s)	260, 787
PluTI	GGCGCC	1 cut(s)	1445
PpsI	GAGTC	2 cut(s)	260, 787
Ppu21I	YACGTR	1 cut(s)	1524
PpuMI	RGGWCCY	1 cut(s)	1643
PshAI	GACNNNNGTC	1 cut(s)	251
Psp124BI	GAGCTC	3 cut(s)	1785, 1821, 1849
Psp5II	RGGWCCY	1 cut(s)	1643
Psp6I	CCWGG	4 cut(s)	686, 959, 1135, 1645
PspEI	GGTNACC	1 cut(s)	986
PspGI	CCWGG	4 cut(s)	686, 959, 1135, 1645
PspN4I	GGNNCC	6 cut(s)	238, 1102, 1443, 1573, 1644, 1662
PspPI	GGNCC	3 cut(s)	1139, 1481, 1643
PspPPI	RGGWCCY	1 cut(s)	1643
PstI	CTGCAG	2 cut(s)	881, 1683
PstNI	CAGNNNCTG	1 cut(s)	1544
PsuI	RGATCY	2 cut(s)	236, 1900
PvuII	CAGCTG	2 cut(s)	287, 767
RsaI	GTAC	3 cut(s)	84, 271, 1292
RsaNI	GTAC	3 cut(s)	83, 270, 1291
RseI	CAYNNNNRTG	3 cut(s)	915, 996, 1509
SacI	GAGCTC	3 cut(s)	1785, 1821, 1849
SapI	GCTCTTC	1 cut(s)	1739
SaqAI	TTAA	5 cut(s)	33, 576, 681, 1257, 2034
SatI	GCNGC	9 cut(s)	38, 342, 375, 471, 1124, 1221, 1341, 1383, 1887
Sau3AI	GATC	9 cut(s)	157, 186, 236, 582, 658, 799, 925, 1798, 1900
Sau96I	GGNCC	3 cut(s)	1139, 1481, 1643
ScaI	AGTACT	1 cut(s)	1292
SchI	GAGTC	2 cut(s)	261, 787
ScrFI	CCNGG	6 cut(s)	241, 688, 785, 961, 1137, 1647
SduI	GDGCHC	4 cut(s)	1576, 1785, 1821, 1849
SfaNI	GCATC	2 cut(s)	1601, 1930
SfcI	CTRYAG	3 cut(s)	877, 1679, 2028
SfoI	GGCGCC	1 cut(s)	1443
Sfr274I	CTCGAG	1 cut(s)	944
SinI	GGWCC	3 cut(s)	1139, 1481, 1643
SlaI	CTCGAG	1 cut(s)	944
SmiMI	CAYNNNNRTG	3 cut(s)	915, 996, 1509
SmlI	CTYRAG	3 cut(s)	41, 944, 1256
SmoI	CTYRAG	3 cut(s)	41, 944, 1256
SpeI	ACTAGT	2 cut(s)	112, 1471
Sse9I	AATT	5 cut(s)	48, 401, 1043, 1597, 1789
SsiI	CCGC	7 cut(s)	155, 165, 1124, 1220, 1467, 1547, 1935
SspDI	GGCGCC	1 cut(s)	1441
SspMI	CTAG	8 cut(s)	113, 249, 530, 647, 713, 1367, 1472, 1718
SstI	GAGCTC	3 cut(s)	1785, 1821, 1849
StyD4I	CCNGG	6 cut(s)	239, 686, 783, 959, 1135, 1645
StyI	CCWWGG	1 cut(s)	1215
TaiI	ACGT	3 cut(s)	104, 1526, 1965
TaqI	TCGA	5 cut(s)	945, 951, 1195, 1588, 1753
TaqII	GACCGA	2 cut(s)	632, 918
TasI	AATT	5 cut(s)	48, 401, 1043, 1597, 1789
TatI	WGTACW	2 cut(s)	82, 1290
TauI	GCSGC	2 cut(s)	1126, 1223
TfiI	GAWTC	5 cut(s)	62, 695, 830, 920, 1500
Tru1I	TTAA	5 cut(s)	33, 576, 681, 1257, 2034
Tru9I	TTAA	5 cut(s)	33, 576, 681, 1257, 2034
TscAI	CASTG	2 cut(s)	1125, 1237
TseFI	GTSAC	2 cut(s)	790, 986
TseI	GCWGC	7 cut(s)	37, 341, 374, 470, 1340, 1382, 1886
Tsp45I	GTSAC	2 cut(s)	790, 986
TspGWI	ACGGA	1 cut(s)	509
TspRI	CASTG	2 cut(s)	1125, 1237
Vha464I	CTTAAG	1 cut(s)	1256
VpaK11BI	GGWCC	3 cut(s)	1139, 1481, 1643
XagI	CCTNNNNNAGG	1 cut(s)	1668
XceI	RCATGY	1 cut(s)	1085
XhoI	CTCGAG	1 cut(s)	944
XmiI	GTMKAC	1 cut(s)	1399
XspI	CTAG	8 cut(s)	113, 249, 530, 647, 713, 1367, 1472, 1718
ZraI	GACGTC	2 cut(s)	102, 1963
ZrmI	AGTACT	1 cut(s)	1292

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.